ClCG08G003860 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG08G003860
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	Pectin lyase-like superfamily protein
Location	CG_Chr08: 11301839 .. 11303026 (-)
RNA-Seq Expression	ClCG08G003860
Synteny	ClCG08G003860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATCCTTAGAAGCCTTTTAGTGCTTTACTTTTCCTGTGTGTTTCTCAACTCAAAATGTGCCATTGCATTGTCATATAATATTGTTAGTTTTGGAGCTAAAGCTGATGGCAAATCTGATTCAACACACGCATTTCAAAAGGCATGGGCCAATGCATGTGGGTCCATTAAACCTGCCTCCATTTATGTGCCCCAAGGAAGATTTTACCTCCAAAGTGGAACATTCAACGGACCTTGCAAGAATAATGCCATTTTCATACGCGTTGATGGCACTCTAGTGGCTCCCTTTGATTATCGAGTTATTGGCAGCTCTCCAGCTTGGATCATGTTTAAGAATGTTGATGGAGTAACTATCTCAGGTGGCATTCTTGATGGTCAAGGAACTAGCTTATGGGCTTGTAAAAATGCTGCCAAGACTTGCCCAACAGGTGCCTCGGTATGTAAAATAATTAAATAATCAATCAACAAGACAATCGTTTACTATGTTTATAATTATTTGTATTCTCTTAAGACAAGTTTCTGTTTTTATTTTAGAAACATTACGTACAAGTGGCCATGTTCTTACCAAGAAAACTATTGGTTGTACCCATAACATGCATTTACATTTCTCTTACATTAAGTAATTTTCTTTTTACTTTTATGTGCAGACGCTCGAATTTTCCTACTCAAAGAATGTAGTGATTAGTGCATTAACATCACTCAAAAGTCAAATGTTCCACATTGTGATCAATCATTGCCAAAATGTGAAAATGAAAGGGCTTAAGGTCTTTGCCCCTGGTGATAGCCCCAACACTGATGGTATACACGTACAACTATCATCCGATGTGACTATACTAAACTCTAAGATAGGCACGGGAGATGACTGCATATCCATCGGTCCTGGCACCTTCAACTTATGGGTTGAGAACATTATATGCGGACCCGGTCATGGAATTAGGTGTGTCTTCTTATCATGATTTTATAGATGTGTAAATACATAAGGAAAATATAAAATTTTATCATTGTGTTAATGTATCTTGGTTGGTTTTGAAGTATTGGAAGTCTAGCAAAGGATGCACAAGAGCCTGGGGTGCAAAATATTACTGTGAAAAATGCTTTATTTTCACGCACTCAGAACGGAGTGAGAATCAAAGCATGGGCTAGGCCAAGCAATGGATTTGTCAGGAGCGTTCTCTTCCAAAACATT

mRNA sequence

ATGGCCATCCTTAGAAGCCTTTTAGTGCTTTACTTTTCCTGTGTGTTTCTCAACTCAAAATGTGCCATTGCATTGTCATATAATATTGTTAGTTTTGGAGCTAAAGCTGATGGCAAATCTGATTCAACACACGCATTTCAAAAGGCATGGGCCAATGCATGTGGGTCCATTAAACCTGCCTCCATTTATGTGCCCCAAGGAAGATTTTACCTCCAAAGTGGAACATTCAACGGACCTTGCAAGAATAATGCCATTTTCATACGCGTTGATGGCACTCTAGTGGCTCCCTTTGATTATCGAGTTATTGGCAGCTCTCCAGCTTGGATCATGTTTAAGAATGTTGATGGAGTAACTATCTCAGGTGGCATTCTTGATGGTCAAGGAACTAGCTTATGGGCTTGTAAAAATGCTGCCAAGACTTGCCCAACAGGTGCCTCGGATGCACAAGAGCCTGGGGTGCAAAATATTACTGTGAAAAATGCTTTATTTTCACGCACTCAGAACGGAGTGAGAATCAAAGCATGGGCTAGGCCAAGCAATGGATTTGTCAGGAGCGTTCTCTTCCAAAACATT

Coding sequence (CDS)

ATGGCCATCCTTAGAAGCCTTTTAGTGCTTTACTTTTCCTGTGTGTTTCTCAACTCAAAATGTGCCATTGCATTGTCATATAATATTGTTAGTTTTGGAGCTAAAGCTGATGGCAAATCTGATTCAACACACGCATTTCAAAAGGCATGGGCCAATGCATGTGGGTCCATTAAACCTGCCTCCATTTATGTGCCCCAAGGAAGATTTTACCTCCAAAGTGGAACATTCAACGGACCTTGCAAGAATAATGCCATTTTCATACGCGTTGATGGCACTCTAGTGGCTCCCTTTGATTATCGAGTTATTGGCAGCTCTCCAGCTTGGATCATGTTTAAGAATGTTGATGGAGTAACTATCTCAGGTGGCATTCTTGATGGTCAAGGAACTAGCTTATGGGCTTGTAAAAATGCTGCCAAGACTTGCCCAACAGGTGCCTCGGATGCACAAGAGCCTGGGGTGCAAAATATTACTGTGAAAAATGCTTTATTTTCACGCACTCAGAACGGAGTGAGAATCAAAGCATGGGCTAGGCCAAGCAATGGATTTGTCAGGAGCGTTCTCTTCCAAAACATT

Protein sequence

MAILRSLLVLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPASIYVPQGRFYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTISGGILDGQGTSLWACKNAAKTCPTGASDAQEPGVQNITVKNALFSRTQNGVRIKAWARPSNGFVRSVLFQNI

Homology

[Close]

BLAST of ClCG08G003860 vs. NCBI nr
Match: XP_022927889.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 258.1 bits (658), Expect = 6.1e-65
Identity = 141/294 (47.96%), Postives = 165/294 (56.12%), Query Frame = 0


Query: 1   MAILRSLLVLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPA 60
           M ILR  L+++F  +FLNS  AIA S N+VS GAK DGKSDST AF+ AWANACGSI+P 
Sbjct: 1   MTILRGFLLVFFPFMFLNSSSAIAFSNNVVSLGAKPDGKSDSTKAFETAWANACGSIRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNGPCKNNAIFIR+DGTLVAP DY+VIG+S AWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSVTFNGPCKNNAIFIRIDGTLVAPSDYKVIGNSAAWILFRGVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GGI DG+G+ LWACKN+ KTCP+GA+                                  
Sbjct: 121 GGIFDGKGSGLWACKNSTKTCPSGATTIEISNSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------------------------------------------------------------ 192
                                                                       
Sbjct: 181 MKGLKVLASGNSPNTDGIHVQLSSDVIILNSKIGTGDDCISIGPGTSNLWIENIVCGPGH 240

[Close]

BLAST of ClCG08G003860 vs. NCBI nr
Match: XP_023531792.1 (polygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 255.8 bits (652), Expect = 3.0e-64
Identity = 138/294 (46.94%), Postives = 165/294 (56.12%), Query Frame = 0


Query: 1   MAILRSLLVLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPA 60
           M ILR+ L+L+F  +FLNS  AIA S N++S GAK DGKSDST AF+ AWANACGS +P 
Sbjct: 1   MTILRAFLLLFFPLMFLNSSGAIAFSNNVISLGAKPDGKSDSTQAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNGPCKNNAIFIR+DGTLVAP DY+VIG+S AWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSVTFNGPCKNNAIFIRIDGTLVAPSDYKVIGNSAAWILFRRVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GG+ DG+G+ LWACKN+ KTCP+GA+                                  
Sbjct: 121 GGVFDGKGSGLWACKNSTKTCPSGATTIEISNSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------------------------------------------------------------ 192
                                                                       
Sbjct: 181 MKGLKVLASGNSPNTDGIHVQLSSDVTILNSKIGTGDDCISIGPGTSNLWIENIVCGPGH 240

[Close]

BLAST of ClCG08G003860 vs. NCBI nr
Match: XP_022927702.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 253.8 bits (647), Expect = 1.2e-63
Identity = 139/294 (47.28%), Postives = 164/294 (55.78%), Query Frame = 0


Query: 1   MAILRSLLVLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPA 60
           M+ILR  L+L+F  +FL S  AI+ S N+VS GAK DGKSDST AF+ AWANACGS +P 
Sbjct: 1   MSILRGFLLLFFPFMFLYSSSAISFSNNVVSLGAKPDGKSDSTQAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNGPCKNNAIFIR+DGTLVAP DYRVIG+S AWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSVTFNGPCKNNAIFIRIDGTLVAPSDYRVIGNSAAWILFRGVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GGI DG+G+ LWACKN+ KTCP+GA+                                  
Sbjct: 121 GGIFDGKGSGLWACKNSTKTCPSGATTIEISDSKNVVLSALTSFNSQMFHIVVNGCQNIK 180

Query: 181 ------------------------------------------------------------ 192
                                                                       
Sbjct: 181 MKGLKVLASGNSPNTDGIHVQLSSDVTILNSKIGTGDDCISIGPGTSNLWIENIVCGPGH 240

[Close]

BLAST of ClCG08G003860 vs. NCBI nr
Match: XP_022989659.1 (polygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 253.1 bits (645), Expect = 2.0e-63
Identity = 138/294 (46.94%), Postives = 163/294 (55.44%), Query Frame = 0


Query: 1   MAILRSLLVLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPA 60
           M ILR  L+L+F  VFL S  AIA S N+V  GAK DGKSDST AF+ AWANACGS +P 
Sbjct: 1   MTILRGFLLLFFPFVFLYSSSAIAFSNNVVDLGAKPDGKSDSTKAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNGPCKNNAIFIR+DGTLVAP DYRVIG+S AWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSLTFNGPCKNNAIFIRIDGTLVAPSDYRVIGNSAAWILFRRVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GG+ DG+G+ LWACKN+ +TCP+GA+                                  
Sbjct: 121 GGVFDGKGSGLWACKNSTRTCPSGATTIEISNSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------------------------------------------------------------ 192
                                                                       
Sbjct: 181 MKGLVVLASGNSPNTDGIHVQLSSDVTILNSKIGTGDDCISIGPGTSSLWIENIVCGPGH 240

[Close]

BLAST of ClCG08G003860 vs. NCBI nr
Match: XP_022927888.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 252.7 bits (644), Expect = 2.6e-63
Identity = 138/294 (46.94%), Postives = 163/294 (55.44%), Query Frame = 0


Query: 1   MAILRSLLVLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPA 60
           M+ILR  L+L+F  +FL S  AIA S N+VS GAK DGKSDST AF+ AWANACGS +P 
Sbjct: 1   MSILRGFLLLFFPFMFLYSSSAIAFSNNVVSLGAKPDGKSDSTKAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNGPCKNNAIFIR+DGTLVAP DYRVIG+S AWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSVTFNGPCKNNAIFIRIDGTLVAPSDYRVIGNSAAWILFRGVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GG+ DG+G+ LWACKN+ KTCP+GA+                                  
Sbjct: 121 GGVFDGKGSGLWACKNSTKTCPSGATTIEISHSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------------------------------------------------------------ 192
                                                                       
Sbjct: 181 IKGLKVLALGNSPNTDGIHVQLSSDVIILNSKIGTGDDCISIGPGTSNLWIENIVCGPGH 240

[Close]

BLAST of ClCG08G003860 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 5.4e-48
Identity = 117/295 (39.66%), Postives = 143/295 (48.47%), Query Frame = 0


Query: 1   MAILRSLLVLYFSCVF-LNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKP 60
           MA  RSL  L    VF +NS  A  L+YN+ S GAKADGK+DST AF  AWA AC S+ P
Sbjct: 1   MANRRSLFSLSLIFVFMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNP 60

Query: 61  ASIYVPQGRFYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTI 120
             IYVP G F+L+   F+GPCKNNAI  R+ GTLVAP DYRVIG++  WI F +V+GVTI
Sbjct: 61  GVIYVPAGTFFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTI 120

Query: 121 SGGILDGQGTSLWACKNA-AKTCPTGAS-------------------------------- 180
           SGGILDGQGT+LWACK    ++CP+GA+                                
Sbjct: 121 SGGILDGQGTALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQN 180

Query: 181 ------------------------------------------------------------ 191
                                                                       
Sbjct: 181 VQMQGVRVSRSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGP 240

[Close]

BLAST of ClCG08G003860 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 6.0e-31
Identity = 93/297 (31.31%), Postives = 123/297 (41.41%), Query Frame = 0


Query: 6   SLLVLYFSCVFLNSKCAIALSY------NIVSFGAKADGKSDSTHAFQKAWANACGSIKP 65
           S L+L  + + L+     +L++      N++S+GAK DG  DST AF  AW  AC S  P
Sbjct: 6   SCLILPLALIILDFTLISSLAHPIPSTLNVLSYGAKPDGSKDSTKAFLAAWDVACASANP 65

Query: 66  ASIYVPQGRFYLQSGTFNG-PCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVT 125
            +I VP+GRF + +  F+G  CK   I IR+ G++VAP D+R+I SS  WI F++V  V+
Sbjct: 66  TTIIVPKGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWFEDVTDVS 125

Query: 126 ISGGILDGQGTSLWACK-NAAKTCPTGASD------------------------------ 185
           I GGILD QGTSLW CK N    CPTGA                                
Sbjct: 126 IYGGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIVIDNSN 185

Query: 186 ------------------------------------------------------------ 192
                                                                       
Sbjct: 186 NVNIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQTIRCG 245

[Close]

BLAST of ClCG08G003860 vs. ExPASy Swiss-Prot
Match: P26216 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 5.8e-18
Identity = 47/115 (40.87%), Postives = 67/115 (58.26%), Query Frame = 0


Query: 26  SYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPASIYVPQGRFYLQSGTFNGPCKNNAI 85
           S++I   GA  +GK+DST A Q+AWA+ACG     +I +P+G F +    F GPCK + +
Sbjct: 40  SFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGD-V 99

Query: 86  FIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTISG-GILDGQGTSLWACKNAAK 140
            I+VDG L+A  D         WI    VD + I+G G LDGQG ++W+  +  K
Sbjct: 100 TIQVDGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTK 153

[Close]

BLAST of ClCG08G003860 vs. ExPASy Swiss-Prot
Match: P35338 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 5.8e-18
Identity = 47/115 (40.87%), Postives = 67/115 (58.26%), Query Frame = 0


Query: 26  SYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPASIYVPQGRFYLQSGTFNGPCKNNAI 85
           S++I   GA  +GK+DST A Q+AWA+ACG     +I +P+G F +    F GPCK + +
Sbjct: 40  SFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGD-V 99

Query: 86  FIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTISG-GILDGQGTSLWACKNAAK 140
            I+VDG L+A  D         WI    VD + I+G G LDGQG ++W+  +  K
Sbjct: 100 TIQVDGNLLATTDLSQYKEHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTK 153

[Close]

BLAST of ClCG08G003860 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.2e-17
Identity = 44/114 (38.60%), Postives = 64/114 (56.14%), Query Frame = 0


Query: 27  YNIVSFGAKADGKSDSTHAFQKAWANACGSIKPASIYVPQGRFYLQSGTFNGPCKNNAIF 86
           +N+  +GAK  G  D + A  KAW  AC S  P+++ +P+G + +      GPCK + I 
Sbjct: 10  FNVNDYGAK--GAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSKIG 69

Query: 87  IRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTISG-GILDGQGTSLWACKNAAK 140
            ++DG + AP D      S  W+ F  +DG+T+SG G LDGQG + WA  N  K
Sbjct: 70  FQIDGVVKAPADPSKF-KSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDK 120

[Close]

BLAST of ClCG08G003860 vs. ExPASy TrEMBL
Match: A0A6J1EJA0 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434653 PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 3.0e-65
Identity = 141/294 (47.96%), Postives = 165/294 (56.12%), Query Frame = 0


Query: 1   MAILRSLLVLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPA 60
           M ILR  L+++F  +FLNS  AIA S N+VS GAK DGKSDST AF+ AWANACGSI+P 
Sbjct: 1   MTILRGFLLVFFPFMFLNSSSAIAFSNNVVSLGAKPDGKSDSTKAFETAWANACGSIRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNGPCKNNAIFIR+DGTLVAP DY+VIG+S AWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSVTFNGPCKNNAIFIRIDGTLVAPSDYKVIGNSAAWILFRGVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GGI DG+G+ LWACKN+ KTCP+GA+                                  
Sbjct: 121 GGIFDGKGSGLWACKNSTKTCPSGATTIEISNSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------------------------------------------------------------ 192
                                                                       
Sbjct: 181 MKGLKVLASGNSPNTDGIHVQLSSDVIILNSKIGTGDDCISIGPGTSNLWIENIVCGPGH 240

[Close]

BLAST of ClCG08G003860 vs. ExPASy TrEMBL
Match: A0A6J1EHY1 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434520 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 5.6e-64
Identity = 139/294 (47.28%), Postives = 164/294 (55.78%), Query Frame = 0


Query: 1   MAILRSLLVLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPA 60
           M+ILR  L+L+F  +FL S  AI+ S N+VS GAK DGKSDST AF+ AWANACGS +P 
Sbjct: 1   MSILRGFLLLFFPFMFLYSSSAISFSNNVVSLGAKPDGKSDSTQAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNGPCKNNAIFIR+DGTLVAP DYRVIG+S AWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSVTFNGPCKNNAIFIRIDGTLVAPSDYRVIGNSAAWILFRGVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GGI DG+G+ LWACKN+ KTCP+GA+                                  
Sbjct: 121 GGIFDGKGSGLWACKNSTKTCPSGATTIEISDSKNVVLSALTSFNSQMFHIVVNGCQNIK 180

Query: 181 ------------------------------------------------------------ 192
                                                                       
Sbjct: 181 MKGLKVLASGNSPNTDGIHVQLSSDVTILNSKIGTGDDCISIGPGTSNLWIENIVCGPGH 240

[Close]

BLAST of ClCG08G003860 vs. ExPASy TrEMBL
Match: A0A6J1JPZ1 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486677 PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 9.5e-64
Identity = 138/294 (46.94%), Postives = 163/294 (55.44%), Query Frame = 0


Query: 1   MAILRSLLVLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPA 60
           M ILR  L+L+F  VFL S  AIA S N+V  GAK DGKSDST AF+ AWANACGS +P 
Sbjct: 1   MTILRGFLLLFFPFVFLYSSSAIAFSNNVVDLGAKPDGKSDSTKAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNGPCKNNAIFIR+DGTLVAP DYRVIG+S AWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSLTFNGPCKNNAIFIRIDGTLVAPSDYRVIGNSAAWILFRRVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GG+ DG+G+ LWACKN+ +TCP+GA+                                  
Sbjct: 121 GGVFDGKGSGLWACKNSTRTCPSGATTIEISNSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------------------------------------------------------------ 192
                                                                       
Sbjct: 181 MKGLVVLASGNSPNTDGIHVQLSSDVTILNSKIGTGDDCISIGPGTSSLWIENIVCGPGH 240

[Close]

BLAST of ClCG08G003860 vs. ExPASy TrEMBL
Match: A0A6J1EMB1 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434652 PE=3 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.2e-63
Identity = 138/294 (46.94%), Postives = 163/294 (55.44%), Query Frame = 0


Query: 1   MAILRSLLVLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPA 60
           M+ILR  L+L+F  +FL S  AIA S N+VS GAK DGKSDST AF+ AWANACGS +P 
Sbjct: 1   MSILRGFLLLFFPFMFLYSSSAIAFSNNVVSLGAKPDGKSDSTKAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNGPCKNNAIFIR+DGTLVAP DYRVIG+S AWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSVTFNGPCKNNAIFIRIDGTLVAPSDYRVIGNSAAWILFRGVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GG+ DG+G+ LWACKN+ KTCP+GA+                                  
Sbjct: 121 GGVFDGKGSGLWACKNSTKTCPSGATTIEISHSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------------------------------------------------------------ 192
                                                                       
Sbjct: 181 IKGLKVLALGNSPNTDGIHVQLSSDVIILNSKIGTGDDCISIGPGTSNLWIENIVCGPGH 240

[Close]

BLAST of ClCG08G003860 vs. ExPASy TrEMBL
Match: A0A6J1JGG6 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486679 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.8e-63
Identity = 137/294 (46.60%), Postives = 162/294 (55.10%), Query Frame = 0


Query: 1   MAILRSLLVLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPA 60
           M ILR  L+L+F  VFL S  AIA S N+V  GAK DGKSDST AF+ AWANACGS +P 
Sbjct: 1   MTILRGFLLLFFPFVFLYSSSAIAFSNNVVDLGAKPDGKSDSTKAFETAWANACGSTRPT 60

Query: 61  SIYVPQGRFYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTIS 120
           SIYVP GR+YL+S TFNGPCKNNAIFIR+DGTLVAP DYRVIG+S AWI+F+ VDGVTIS
Sbjct: 61  SIYVPPGRYYLRSLTFNGPCKNNAIFIRIDGTLVAPSDYRVIGNSAAWILFRRVDGVTIS 120

Query: 121 GGILDGQGTSLWACKNAAKTCPTGAS---------------------------------- 180
           GG+ DG+G+ LWACKN+ +TCP+GA+                                  
Sbjct: 121 GGVFDGKGSGLWACKNSTRTCPSGATTIEISNSKNVVLSALTSLNSQMFHIVVNGCQNIK 180

Query: 181 ------------------------------------------------------------ 192
                                                                       
Sbjct: 181 MKGLVVLASGNSPNTDGIHVQLSSDVTILNSKIGTGDDCISIGPGTSSLWVENIVCGPGH 240

[Close]

BLAST of ClCG08G003860 vs. TAIR 10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 172.9 bits (437), Expect = 2.4e-43
Identity = 102/288 (35.42%), Postives = 138/288 (47.92%), Query Frame = 0


Query: 7   LLVLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPASIYVPQ 66
           L+VL F    L+   + A +YNI+S+GAK DGK+DST AF   WA AC S+KP +I VP+
Sbjct: 6   LIVLLF---LLSVSSSSAQTYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILVPK 65

Query: 67  GRFYLQSGTFNG-PCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTISGGILD 126
           GRF L+S  F+G  CK  ++  R+ GTLVAP DYRVIG    WI+F+++DG+++ GG+LD
Sbjct: 66  GRFLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGVLD 125

Query: 127 GQGTSLWACKNAAKTCPTGAS--------------------------------------- 186
            QG SLW+CK + K CP+GA+                                       
Sbjct: 126 AQGASLWSCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHVAINGCSNVKLDGVK 185

Query: 187 ------------------------------------------------------------ 191
                                                                       
Sbjct: 186 VSADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWIENVACGPGHGISIG 245

[Close]

BLAST of ClCG08G003860 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 172.6 bits (436), Expect = 3.1e-43
Identity = 103/291 (35.40%), Postives = 137/291 (47.08%), Query Frame = 0


Query: 4   LRSLLVLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPASIY 63
           + SLLVL+    F  S C+ A SYN++SFGAK DGK+D+T AF   W  AC S +P +I 
Sbjct: 1   MASLLVLF--VFFFISSCS-AQSYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIV 60

Query: 64  VPQGRFYLQSGTFNG-PCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTISGG 123
           VP+GRF L+S TF+G  CK   +  R+DGTLVAP DYRVIG+   WI F+++DG+T+ GG
Sbjct: 61  VPKGRFLLRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGG 120

Query: 124 ILDGQGTSLWACKNAAKTCPTGAS------------------------------------ 183
           +LD +G SLW CK + K CP+GA+                                    
Sbjct: 121 VLDARGASLWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHVVINGCNNVKLQ 180

Query: 184 ------------------------------------------------------------ 191
                                                                       
Sbjct: 181 GVKVLAAGNSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWIENVACGPGHGI 240

[Close]

BLAST of ClCG08G003860 vs. TAIR 10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 154.5 bits (389), Expect = 8.9e-38
Identity = 95/291 (32.65%), Postives = 130/291 (44.67%), Query Frame = 0


Query: 4   LRSLLVLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPASIY 63
           L ++L+++FS   L      A +YN+VSFGAK DG++DST AF  AW  AC S    ++ 
Sbjct: 6   LVAVLLMFFSSFLLMKSSTAASNYNVVSFGAKPDGRTDSTKAFLGAWQAACRSAAAVTVT 65

Query: 64  VPQGRFYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTISGGI 123
           VP+G F L+   F GPC++   F ++ GT+VAP DYR +G+S  WI+F  V+ ++I GG 
Sbjct: 66  VPRGSFLLKPVEFRGPCRSRITF-QIYGTIVAPSDYRGLGNSGYWILFVKVNRISIIGGT 125

Query: 124 LDGQGTSLWACKNAAKTCPTGA-------------------------------------- 183
           LD +G S WAC+ + K+CP GA                                      
Sbjct: 126 LDARGASFWACRKSGKSCPVGARSMTFNWANDVVVSGLTSINSQTTHLVINSCNNVIVRK 185

Query: 184 ------------------------------------------------------------ 192
                                                                       
Sbjct: 186 VKLVAPDQSPNTDGLHVQGSAGVTVTDGTFHTGDDCISIGPGTRNLYMSKLNCGPGHGIS 245

[Close]

BLAST of ClCG08G003860 vs. TAIR 10
Match: AT1G05650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 149.1 bits (375), Expect = 3.7e-36
Identity = 87/286 (30.42%), Postives = 126/286 (44.06%), Query Frame = 0


Query: 9   VLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPASIYVPQGR 68
           +++    F++   + ++ +N+VSFGAK DG +DST AF KAW  ACGS   A++ VP G 
Sbjct: 10  LIFTLLTFIDVSSSASIVFNVVSFGAKPDGVTDSTAAFLKAWQGACGSAASATVVVPTGT 69

Query: 69  FYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTISGGILDGQG 128
           F L+  TF GPCK+   F +V GT+VAP DYR  G+S +WI+F  V+  ++ GG  D +G
Sbjct: 70  FLLKVITFGGPCKSKITF-QVTGTVVAPEDYRTFGNSGSWILFNKVNRFSLVGGTFDARG 129

Query: 129 TSLWACKNAAKTCPTG-------------------------------------------- 188
           +  W+C+ + + CP G                                            
Sbjct: 130 SGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVSHMTLNGCTNVAVRNIRLVA 189

Query: 189 -----------------------------------------------------------A 192
                                                                      A
Sbjct: 190 PGDSPNTDGFTVQFSTGVTLTGSTVQTGDDCVAIGQGTRNFLISKLACGPGHGVSIGSLA 249

[Close]

BLAST of ClCG08G003860 vs. TAIR 10
Match: AT1G05660.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 142.5 bits (358), Expect = 3.5e-34
Identity = 85/286 (29.72%), Postives = 125/286 (43.71%), Query Frame = 0


Query: 9   VLYFSCVFLNSKCAIALSYNIVSFGAKADGKSDSTHAFQKAWANACGSIKPASIYVPQGR 68
           +L+    F++   + +  +N+VSFGAK DG +DST AF KAW  AC S   A++ VP+G 
Sbjct: 10  LLFTLLTFIDVSISASNVFNVVSFGAKPDGVTDSTGAFLKAWQGACVSASSATVVVPKGT 69

Query: 69  FYLQSGTFNGPCKNNAIFIRVDGTLVAPFDYRVIGSSPAWIMFKNVDGVTISGGILDGQG 128
           F L+  TF GPCK+   F +V GT++AP DYR  G+S  WI+F  V+  ++ GG  D + 
Sbjct: 70  FLLKVITFGGPCKSKITF-QVAGTVIAPEDYRTFGNSGFWILFNKVNRFSLVGGTFDARA 129

Query: 129 TSLWACKNAAKTCPTG-------------------------------------------- 188
              W+C+ + + CP G                                            
Sbjct: 130 NGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVTHMTLNGCTNVVVRNVKLVA 189

Query: 189 -----------------------------------------------------------A 192
                                                                      A
Sbjct: 190 PGNSPNTDGFHVQHSTGVTFTGSTVQTGDDCVAIGPGTRNLLITKLACGPGHGVSIGSLA 249

The following BLAST results are available for this feature:

BLAST of ClCG08G003860 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022927889.1	6.1e-65	47.96	polygalacturonase-like [Cucurbita moschata]	[more]
XP_023531792.1	3.0e-64	46.94	polygalacturonase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022927702.1	1.2e-63	47.28	polygalacturonase-like [Cucurbita moschata]	[more]
XP_022989659.1	2.0e-63	46.94	polygalacturonase-like [Cucurbita maxima]	[more]
XP_022927888.1	2.6e-63	46.94	polygalacturonase-like [Cucurbita moschata]	[more]

BLAST of ClCG08G003860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P48979	5.4e-48	39.66	Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1	[more]
O22818	6.0e-31	31.31	Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386...	[more]
P26216	5.8e-18	40.87	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1	[more]
P35338	5.8e-18	40.87	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1	[more]
Q6H9K0	2.2e-17	38.60	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]

BLAST of ClCG08G003860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EJA0	3.0e-65	47.96	polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434653 PE=3 SV=1	[more]
A0A6J1EHY1	5.6e-64	47.28	polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434520 PE=3 SV=1	[more]
A0A6J1JPZ1	9.5e-64	46.94	polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486677 PE=3 SV=1	[more]
A0A6J1EMB1	1.2e-63	46.94	polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434652 PE=3 SV=1	[more]
A0A6J1JGG6	2.8e-63	46.60	polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486679 PE=3 SV=1	[more]

BLAST of ClCG08G003860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G59850.1	2.4e-43	35.42	Pectin lyase-like superfamily protein	[more]
AT2G43870.1	3.1e-43	35.40	Pectin lyase-like superfamily protein	[more]
AT2G43890.1	8.9e-38	32.65	Pectin lyase-like superfamily protein	[more]
AT1G05650.1	3.7e-36	30.42	Pectin lyase-like superfamily protein	[more]
AT1G05660.1	3.5e-34	29.72	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 28..144 e-value: 9.6E-25 score: 89.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 145..191 e-value: 1.0E-9 score: 39.7
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 151..191 e-value: 1.3E-5 score: 24.4 coord: 54..139 e-value: 1.4E-9 score: 37.5
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 9..144
None	No IPR available	PANTHER	PTHR31375:SF219	PUTATIVE-RELATED	coord: 9..144
None	No IPR available	PANTHER	PTHR31375:SF219	PUTATIVE-RELATED	coord: 146..190
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 146..190
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 14..191

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG08G003860.1	ClCG08G003860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity