ClCG08G003250 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG08G003250
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	Cysteine Protease
Location	CG_Chr08: 8052895 .. 8055316 (+)
RNA-Seq Expression	ClCG08G003250
Synteny	ClCG08G003250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAGGTTTAATCGATTCAAAGTGTTTGAAGAGAATGCGAAGCATGTGCACAAAGTGAATCAAATGAACAAATCCTACAAGTTGAAGTTGAATAGATTTGGTGATATGTCAAATTCTGAGTTTGCGAACTTATATGCTAACTCGAATATTAACTACTATAGAAACTTAAAGAAAACACGAAAGGCTGGTGGCGGAGGATTCATGTATAAGCAAGTCCTGAATCTTCCACCTTCTATTGATTGGAGAGCTATGGGAGCTGTTACTGGTTTGAAGTCCCAAGACTTGGGTGCTATAGGATGTGGTAATTGACCATAATTTATTGCTTAATTTTGATTAATATGTATGTAATATTGATGTTGAATGTGTTCTTTTTTTTAATTATTAATTATTTATGAAAAAAAAGAAAAAAAAATGAATGTGTGTTTTTGTAGGATGTTGTTGGGCATTTTCAGCCATAGGTGCAGTGGAAGGAATAAACCAAATAAGAACAAAGCAATTAGTACCTCTGTCAGAACAAGAGCTCGTGGACTGTGATCAAATGGATGGAGGTTGTGGTGGAGGATACATGGAAACTGCTTTTGATTTCATAAGGCAAAATGGTGGAATTACAACTGAGGCTAATTATCCTTACAACGCTAAACAAGGATATTGTACCTCTAGCTCATCTAGAGTAACTCACACTCACTCACTCATGGATTCACCCACACCCTTTTCCTTCCTAATTTTTAAATATTTATCAAATTAATTAATTAATTAATTTTGCATATGTAGATGAATTTAGTCACAATTGATGGATATCAGAATGTACCTCCATACAATGAAGATGCTTTGATGCAAGCTGTGGCGAACCAACCGGTGTCCGTAGCATTGGAGGGCAGTGGACTTGATTTCCAATTCTACGGGGGGGCAAGTTCTTCTTATAGCTTCTTCTTATGCTTTCTGGTTTTACAGTTTAAAATGGCTAATTTTGAAAAGACTTTTTTTTTTCTTTCTTTTTCTGCAGGGAGTGTTTAATGGAAACTGTGGAGTGGTGCTTAATCATGCAGTGGTAGCAGTTGGATATGAACCAGATTATTGGATAATGAAGAACTCATGGGGAGCTGGATGGGGAGAAGGAGGTTATGTAAGGATGCAACGAGGAGCGAATCCACCGGCTGGGCTATGTGGAATAGCCATTGATGCCTCCTTTCCCATTAAGTACTAGCACTTCTTTCATTAACAAAGTAGCTAGTGTATGTATAGCTAGCTAGGGATCCATTCAATTTTGTTCAAATAAATAATAAGTAGTACGATTTTTGTTTTCCATTTTTTTACTTTTAGACTTTTACTATTCTAGCTTTTCTTTTTTAATTACCTTATTTTAAACAATAATTAATGTTAGAAATGCCAAGAATTTAACTGGGTTTTTTATTTGAGGGCAATTGAATAATTAAAGCCCAATGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATAAAGCTTAATGGTTTTTTTAAAANACTATTCTAGCTTTTCTTTTTTAATTACCTTATTTTAAACAATAATTAATGTTAGAAATGCCAAGAATTTAACTGGGTTTTTTATTTGAGGGCAATTGAATAATTAAAGCCCAATGGTTTTTTTTTTTTTTTTTTTTTTTTTTGTAAATAAAGCTTAATGGTTTTTTTAAAATTAAGTTGATCTTCACCAACTTAGCCACCGTGAGACTAAGAAAAGCCGAGAAAAAAACTTCTTCTTGGGGGAGGTAGCAGCCAATTAACACATCAAGGGAGAGGAAAAGAGAAAGACACTTAGAGAGAGAGATATCCTGTAGAAAGGAAGAAGAAGATCGTGAGTAAGGAAGATATTTTGTTCACCATTGTCAGCGTTCGGTTCGCTGTACCGGCATGGGTTTCTTCATCCTGCACTGGGTCTGTATGCCGTGTGGGCCTCCGGTCAGAAAGGGGTTCTTTTTTAGCATGGGTAGTGTCACACTCCCTCCCGAGTTATCTTTTTAAACTCGAGAAGAGATGTGATGACAGGAAACCTTACCCTTTTGTAACATCTACTGCCCTCCTTTTTTGCTTTACATACTTAACATGCACAAACTTAATAAACACTTTTATTAAGCATCAACTTAAAGCTTATACAATCTTACAAAATTACCACAACTCCGACCTTCAATCGTAGATACAGTAAGCTATCCTATGGCATACCTCAACTTTGCATGGTAACATATAACTAGGGTACCCACGACCCTTAATAATACCATGATATAGAAGCAATGATCAAGAAAAAACAAATCAACCACAGCCGAGGTTGTTTACTCAAACCTTCTGTACCTTATTGCTCATAGAATTAGGAAGTGGTTTATGGTAACCCCATAGAATTAGGAAGTAGGTTTCTGGTAACCCCTATGAAGGAATCGAATTGGAAGTGA

mRNA sequence

ATGGAGAGGTTTAATCGATTCAAAGTGTTTGAAGAGAATGCGAAGCATGTGCACAAAGTGAATCAAATGAACAAATCCTACAAGTTGAAGTTGAATAGATTTGGTGATATGTCAAATTCTGAGTTTGCGAACTTATATGCTAACTCGAATATTAACTACTATAGAAACTTAAAGAAAACACGAAAGGCTGGTGGCGGAGGATTCATGTATAAGCAAGTCCTGAATCTTCCACCTTCTATTGATTGGAGAGCTATGGGAGCTGTTACTGGTTTGAAGTCCCAAGACTTGGGTGCTATAGGATGTGGATGTTGTTGGGCATTTTCAGCCATAGGTGCAGTGGAAGGAATAAACCAAATAAGAACAAAGCAATTAGTACCTCTGTCAGAACAAGAGCTCGTGGACTGTGATCAAATGGATGGAGGTTGTGGTGGAGGATACATGGAAACTGCTTTTGATTTCATAAGGCAAAATGGTGGAATTACAACTGAGGCTAATTATCCTTACAACGCTAAACAAGGATATTGTACCTCTAGCTCATCTAGAATGAATTTAGTCACAATTGATGGATATCAGAATGTACCTCCATACAATGAAGATGCTTTGATGCAAGCTGTGGCGAACCAACCGGTGTCCGTAGCATTGGAGGGCAGTGGACTTGATTTCCAATTCTACGGGGGGGCAAGTTCTTCTTATAGCTTCTTCTTATGCTTTCTGGGAGTGTTTAATGGAAACTGTGGAGTGGTGCTTAATCATGCAGTGGTAGCAGTTGGATATGAACCAGATTATTGGATAATGAAGAACTCATGGGGAGCTGGATGGGGAGAAGGAGGTTATGTAAGGATGCAACGAGGAGCGAATCCACCGGCTGGGCTATGTGGAATAGCCATTGATGCCTCCTTTCCCATTAAGAAGAAGAAGATCGTGAGTAAGGAAGATATTTTGTTCACCATTGTCAGCGTTCGGTTCGCTGTACCGGCATGGGTTTCTTCATCCTGCACTGGGTCTGTATGCCGTGTGGGCCTCCGGTCAGAAAGGGGTTCTTTTTTAGCATGGGAAGTGGTTTATGGTAACCCCATAGAATTAGGAAGTAGGTTTCTGGTAACCCCTATGAAGGAATCGAATTGGAAGTGA

Coding sequence (CDS)

ATGGAGAGGTTTAATCGATTCAAAGTGTTTGAAGAGAATGCGAAGCATGTGCACAAAGTGAATCAAATGAACAAATCCTACAAGTTGAAGTTGAATAGATTTGGTGATATGTCAAATTCTGAGTTTGCGAACTTATATGCTAACTCGAATATTAACTACTATAGAAACTTAAAGAAAACACGAAAGGCTGGTGGCGGAGGATTCATGTATAAGCAAGTCCTGAATCTTCCACCTTCTATTGATTGGAGAGCTATGGGAGCTGTTACTGGTTTGAAGTCCCAAGACTTGGGTGCTATAGGATGTGGATGTTGTTGGGCATTTTCAGCCATAGGTGCAGTGGAAGGAATAAACCAAATAAGAACAAAGCAATTAGTACCTCTGTCAGAACAAGAGCTCGTGGACTGTGATCAAATGGATGGAGGTTGTGGTGGAGGATACATGGAAACTGCTTTTGATTTCATAAGGCAAAATGGTGGAATTACAACTGAGGCTAATTATCCTTACAACGCTAAACAAGGATATTGTACCTCTAGCTCATCTAGAATGAATTTAGTCACAATTGATGGATATCAGAATGTACCTCCATACAATGAAGATGCTTTGATGCAAGCTGTGGCGAACCAACCGGTGTCCGTAGCATTGGAGGGCAGTGGACTTGATTTCCAATTCTACGGGGGGGCAAGTTCTTCTTATAGCTTCTTCTTATGCTTTCTGGGAGTGTTTAATGGAAACTGTGGAGTGGTGCTTAATCATGCAGTGGTAGCAGTTGGATATGAACCAGATTATTGGATAATGAAGAACTCATGGGGAGCTGGATGGGGAGAAGGAGGTTATGTAAGGATGCAACGAGGAGCGAATCCACCGGCTGGGCTATGTGGAATAGCCATTGATGCCTCCTTTCCCATTAAGAAGAAGAAGATCGTGAGTAAGGAAGATATTTTGTTCACCATTGTCAGCGTTCGGTTCGCTGTACCGGCATGGGTTTCTTCATCCTGCACTGGGTCTGTATGCCGTGTGGGCCTCCGGTCAGAAAGGGGTTCTTTTTTAGCATGGGAAGTGGTTTATGGTAACCCCATAGAATTAGGAAGTAGGTTTCTGGTAACCCCTATGAAGGAATCGAATTGGAAGTGA

Protein sequence

MERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTRKAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRTKQLVPLSEQELVDCDQMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSSRMNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGVFNGNCGVVLNHAVVAVGYEPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIAIDASFPIKKKKIVSKEDILFTIVSVRFAVPAWVSSSCTGSVCRVGLRSERGSFLAWEVVYGNPIELGSRFLVTPMKESNWK

Homology

[Close]

BLAST of ClCG08G003250 vs. NCBI nr
Match: XP_038885064.1 (LOW QUALITY PROTEIN: vignain-like [Benincasa hispida])

HSP 1 Score: 363.2 bits (931), Expect = 2.7e-96
Identity = 181/307 (58.96%), Postives = 219/307 (71.34%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  RFKVF+EN  HV  VNQMNK YKLKLN+F DMSN EF N YA SNI++YR L   R
Sbjct: 84  EKHKRFKVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHGRR 143

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           + G  GFMY++  +LP  IDWR  GAV G+K Q      CG CWAFS++ AVEGIN+IRT
Sbjct: 144 REGTSGFMYEEATDLPSYIDWRERGAVNGIKEQG----RCGSCWAFSSVAAVEGINKIRT 203

Query: 122 KQLVPLSEQELVDCDQMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSSR 181
            QL+ LSEQEL+DC+  + GC GG+ME AFDFIR+NGGI TE +YPY+  +G C SS   
Sbjct: 204 NQLLSLSEQELLDCNYRNEGCNGGFMEIAFDFIRRNGGIATENSYPYHGSRGLCRSSRIS 263

Query: 182 MNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGVF 241
             +V IDGY+++P  NEDALMQAVANQPVSVA++ +G DFQFY            + GVF
Sbjct: 264 SPIVKIDGYESIPE-NEDALMQAVANQPVSVAIDAAGRDFQFY------------WQGVF 323

Query: 242 NGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIAI 301
           +G CG  LNH VVA+GY       DYWI++NSWG GWGE GYVRM+RG   P GLCGIA+
Sbjct: 324 DGYCGTELNHGVVAIGYGTTEDGTDYWIVRNSWGVGWGEEGYVRMKRGVEQPEGLCGIAM 373

Query: 302 DASFPIK 304
           +AS+PIK
Sbjct: 384 EASYPIK 373

[Close]

BLAST of ClCG08G003250 vs. NCBI nr
Match: XP_008444390.1 (PREDICTED: vignain-like [Cucumis melo])

HSP 1 Score: 355.1 bits (910), Expect = 7.2e-94
Identity = 178/307 (57.98%), Postives = 218/307 (71.01%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  RF VF+EN  HV  VNQMNK YKLKLN+F DMSN EF N YA SNI+++R L   R
Sbjct: 56  EKHKRFSVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNFYARSNISHFRKL-HGR 115

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           + G GGFMY+Q  +LP S+DWR  GAV  +K Q      CG CWAFS++ AVE IN+I+T
Sbjct: 116 RRGAGGFMYEQDTDLPSSVDWRERGAVNAIKEQGT----CGSCWAFSSVAAVEAINKIKT 175

Query: 122 KQLVPLSEQELVDCDQMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSSR 181
            QL+ LSEQEL+DC+  + GC GG+ME AFDFI++NGGI TE +YPY+  +G C SS   
Sbjct: 176 NQLLSLSEQELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRIS 235

Query: 182 MNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGVF 241
             +V IDGY++VP  NEDALMQAVANQPVSVA++ +G DFQFY            + GVF
Sbjct: 236 SPIVKIDGYESVPE-NEDALMQAVANQPVSVAIDAAGRDFQFY------------WQGVF 295

Query: 242 NGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIAI 301
           +G CG  LNH VVA+GY       DYWI++NSWG GWGE GYVRM+RG   P GLCGIA+
Sbjct: 296 DGYCGTELNHGVVAIGYGTTEDGTDYWIVRNSWGVGWGEDGYVRMKRGVEQPEGLCGIAM 344

Query: 302 DASFPIK 304
           +AS+PIK
Sbjct: 356 EASYPIK 344

[Close]

BLAST of ClCG08G003250 vs. NCBI nr
Match: XP_030957117.1 (vignain-like [Quercus lobata])

HSP 1 Score: 354.8 bits (909), Expect = 9.4e-94
Identity = 179/322 (55.59%), Postives = 224/322 (69.57%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  RF VF+EN KHVHKVN+MNK YKLKLN+F DM+N EF++ YA+S ++++R  +  R
Sbjct: 55  EKHKRFNVFKENVKHVHKVNKMNKPYKLKLNKFADMTNHEFSSSYASSKVSHHRMFQGIR 114

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           +    GFM++++ NLP S+DWR  GAVTG+K Q      CG CWAFS + AVEGINQI+T
Sbjct: 115 QV--TGFMHEKIDNLPTSVDWRKQGAVTGVKDQG----KCGSCWAFSTVVAVEGINQIKT 174

Query: 122 KQLVPLSEQELVDCDQMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSSR 181
           K+LVPLSEQELVDCD  + GC GG ME AF+FI+Q GG+TTE NYPY A+   C SS   
Sbjct: 175 KELVPLSEQELVDCDSKNNGCDGGLMENAFEFIKQTGGVTTEKNYPYQARHKSCDSSKMN 234

Query: 182 MNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGVF 241
             +V IDGY+NVP  +E+ALM+AVANQPVSVA++  G DFQFY              GVF
Sbjct: 235 DPMVQIDGYENVPQNDENALMKAVANQPVSVAIDAGGKDFQFYSE------------GVF 294

Query: 242 NGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIAI 301
            G+CG  LNH V  VGY        YWI+KNSWG  WGE GY+RMQRG N   GLCGIA+
Sbjct: 295 TGDCGTELNHGVAIVGYGTTLDGKKYWIVKNSWGPEWGEKGYIRMQRGVNAEEGLCGIAM 354

Query: 302 DASFPIK-----KKKIVSKEDI 314
           +AS+P+K     KKK   K+++
Sbjct: 355 EASYPVKLSSNNKKKTSFKDEL 358

[Close]

BLAST of ClCG08G003250 vs. NCBI nr
Match: XP_023870610.1 (vignain-like [Quercus suber])

HSP 1 Score: 353.2 bits (905), Expect = 2.7e-93
Identity = 179/322 (55.59%), Postives = 222/322 (68.94%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  RF VF+EN KHVHKVN+MNK YKLKLN+F DM+N EFA+ YA+S ++++R  +  R
Sbjct: 55  EKHKRFNVFKENVKHVHKVNKMNKPYKLKLNKFADMTNHEFASSYASSKVSHHRMFQGIR 114

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           +    GFM++++ NLP S+DWR  GAVTG+K Q      CG CWAFS + AVEGINQI+T
Sbjct: 115 QV--TGFMHEKIDNLPTSVDWRKRGAVTGVKDQG----KCGSCWAFSTVVAVEGINQIKT 174

Query: 122 KQLVPLSEQELVDCDQMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSSR 181
            +LVPLSEQELVDCD  + GC GG ME AF+FI+Q GG+TTE NYPY A+   C SS   
Sbjct: 175 NELVPLSEQELVDCDSKNNGCDGGLMENAFEFIKQTGGVTTENNYPYQARHKSCDSSKMN 234

Query: 182 MNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGVF 241
             +V IDGY+NVP  +E+ALMQAVANQPVSVA++  G DFQFY              GVF
Sbjct: 235 APMVQIDGYENVPQNDENALMQAVANQPVSVAIDAGGKDFQFY------------LEGVF 294

Query: 242 NGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIAI 301
            G+C   LNH V  VGY        YWI+KNSWG  WGE GY+RMQRG N   GLCGIA+
Sbjct: 295 TGDCSTELNHGVAIVGYGTTLDGKKYWIVKNSWGPEWGEKGYIRMQRGVNAEEGLCGIAM 354

Query: 302 DASFPIK-----KKKIVSKEDI 314
           +AS+P+K     KKK   K+++
Sbjct: 355 EASYPVKLSSNNKKKTSFKDEL 358

[Close]

BLAST of ClCG08G003250 vs. NCBI nr
Match: XP_004142960.1 (vignain [Cucumis sativus] >KGN62334.1 hypothetical protein Csa_018585 [Cucumis sativus])

HSP 1 Score: 352.8 bits (904), Expect = 3.6e-93
Identity = 177/307 (57.65%), Postives = 218/307 (71.01%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  RF VF+EN  HV  VNQM+K YKLKLN+F DMSN EF N YA SNI++YR L + R
Sbjct: 56  EKHKRFSVFKENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHE-R 115

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           + G GGFMY+Q  +LP S+DWR  GAV  +K Q      CG CWAFS++ AVEGIN+I+T
Sbjct: 116 RRGAGGFMYEQDTDLPSSVDWRERGAVNAVKEQG----RCGSCWAFSSVAAVEGINKIKT 175

Query: 122 KQLVPLSEQELVDCDQMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSSR 181
            QL+ LSEQEL+DC+  + GC GG+ME AFDFI++NGGI TE +YPY+  +G C SS   
Sbjct: 176 NQLLSLSEQELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRIS 235

Query: 182 MNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGVF 241
             +V IDGY++VP  NEDALMQAVANQPVSVA++ +G DFQFY              GVF
Sbjct: 236 SPIVKIDGYESVPE-NEDALMQAVANQPVSVAIDAAGRDFQFYS------------QGVF 295

Query: 242 NGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIAI 301
           +G CG  LNH VVA+GY       DYW+++NSWG GWGE GYVRM+RG     GLCGIA+
Sbjct: 296 DGYCGTELNHGVVAIGYGTTEDGTDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAM 344

Query: 302 DASFPIK 304
           +AS+PIK
Sbjct: 356 EASYPIK 344

[Close]

BLAST of ClCG08G003250 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 323.6 bits (828), Expect = 3.1e-87
Identity = 165/308 (53.57%), Postives = 210/308 (68.18%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  RF VF+ N  HVH  N+M+K YKLKLN+F DM+N EF + YA S +N+ R  + T 
Sbjct: 55  EKHKRFNVFKANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTP 114

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
               G FMY++V+++PPS+DWR  GAVT +K Q      CG CWAFS + AVEGINQI+T
Sbjct: 115 HE-NGAFMYEKVVSVPPSVDWRKKGAVTDVKDQG----QCGSCWAFSTVVAVEGINQIKT 174

Query: 122 KQLVPLSEQELVDCDQMDG-GCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSS 181
            +LV LSEQELVDCD+ +  GC GG ME+AF+FI+Q GGITTE+NYPY A++G C +S  
Sbjct: 175 NKLVALSEQELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKV 234

Query: 182 RMNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGV 241
               V+IDG++NVP  +EDAL++AVANQPVSVA++  G DFQFY              GV
Sbjct: 235 NDLAVSIDGHENVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSE------------GV 294

Query: 242 FNGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIA 301
           F G+C   LNH V  VGY       +YWI++NSWG  WGE GY+RMQR  +   GLCGIA
Sbjct: 295 FTGDCSTDLNHGVAIVGYGTTVDGTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIA 345

Query: 302 IDASFPIK 304
           +  S+PIK
Sbjct: 355 MLPSYPIK 345

[Close]

BLAST of ClCG08G003250 vs. ExPASy Swiss-Prot
Match: O65039 (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 5.2e-87
Identity = 166/309 (53.72%), Postives = 207/309 (66.99%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  RF VF+ NA HVH  N+M+K YKLKLN+F DM+N EF N Y+ S + ++R  +   
Sbjct: 53  EKQKRFNVFKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRGGP 112

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           + G G FMY++V  +P S+DWR  GAVT +K Q      CG CWAFS I AVEGINQI+T
Sbjct: 113 R-GNGTFMYEKVDTVPASVDWRKKGAVTSVKDQG----QCGSCWAFSTIVAVEGINQIKT 172

Query: 122 KQLVPLSEQELVDCD-QMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSS 181
            +LV LSEQELVDCD   + GC GG M+ AF+FI+Q GGITTEANYPY A  G C  S  
Sbjct: 173 NKLVSLSEQELVDCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKE 232

Query: 182 RMNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGV 241
               V+IDG++NVP  +E+AL++AVANQPVSVA++  G DFQFY              GV
Sbjct: 233 NAPAVSIDGHENVPENDENALLKAVANQPVSVAIDAGGSDFQFYSE------------GV 292

Query: 242 FNGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIA 301
           F G+CG  L+H V  VGY        YW +KNSWG  WGE GY+RM+RG +   GLCGIA
Sbjct: 293 FTGSCGTELDHGVAIVGYGTTIDGTKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIA 344

Query: 302 IDASFPIKK 305
           ++AS+PIKK
Sbjct: 353 MEASYPIKK 344

[Close]

BLAST of ClCG08G003250 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 2.0e-86
Identity = 164/308 (53.25%), Postives = 211/308 (68.51%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  RF VF+ N  HVH  N+M+K YKLKLN+F DM+N EF + YA S +N+++  + ++
Sbjct: 55  EKHKRFNVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQ 114

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
             G G FMY++V ++P S+DWR  GAVT +K Q      CG CWAFS I AVEGINQI+T
Sbjct: 115 H-GSGTFMYEKVGSVPASVDWRKKGAVTDVKDQG----QCGSCWAFSTIVAVEGINQIKT 174

Query: 122 KQLVPLSEQELVDCDQMDG-GCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSS 181
            +LV LSEQELVDCD+ +  GC GG ME+AF+FI+Q GGITTE+NYPY A++G C  S  
Sbjct: 175 NKLVSLSEQELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKV 234

Query: 182 RMNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGV 241
               V+IDG++NVP  +E+AL++AVANQPVSVA++  G DFQFY              GV
Sbjct: 235 NDLAVSIDGHENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSE------------GV 294

Query: 242 FNGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIA 301
           F G+C   LNH V  VGY       +YWI++NSWG  WGE GY+RMQR  +   GLCGIA
Sbjct: 295 FTGDCNTDLNHGVAIVGYGTTVDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIA 345

Query: 302 IDASFPIK 304
           + AS+PIK
Sbjct: 355 MMASYPIK 345

[Close]

BLAST of ClCG08G003250 vs. ExPASy Swiss-Prot
Match: Q9FGR9 (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 PE=1 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 6.4e-85
Identity = 162/308 (52.60%), Postives = 204/308 (66.23%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  RF VF+ N KH+H+ N+ +KSYKLKLN+FGDM++ EF   YA SNI ++R  +  +
Sbjct: 53  EKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEK 112

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           KA    FMY  V  LP S+DWR  GAVT +K+Q      CG CWAFS + AVEGINQIRT
Sbjct: 113 KA-TKSFMYANVNTLPTSVDWRKNGAVTPVKNQG----QCGSCWAFSTVVAVEGINQIRT 172

Query: 122 KQLVPLSEQELVDCD-QMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSS 181
           K+L  LSEQELVDCD   + GC GG M+ AF+FI++ GG+T+E  YPY A    C ++  
Sbjct: 173 KKLTSLSEQELVDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKE 232

Query: 182 RMNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGV 241
              +V+IDG+++VP  +ED LM+AVANQPVSVA++  G DFQFY              GV
Sbjct: 233 NAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSE------------GV 292

Query: 242 FNGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIA 301
           F G CG  LNH V  VGY        YWI+KNSWG  WGE GY+RMQRG     GLCGIA
Sbjct: 293 FTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIA 343

Query: 302 IDASFPIK 304
           ++AS+P+K
Sbjct: 353 MEASYPLK 343

[Close]

BLAST of ClCG08G003250 vs. ExPASy Swiss-Prot
Match: Q9STL4 (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 8.3e-85
Identity = 163/309 (52.75%), Postives = 209/309 (67.64%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           ER  RF VF  N  HVH  N+ N+SYKLKLN+F D++ +EF N Y  SNI ++R L+   
Sbjct: 53  EREKRFNVFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGP- 112

Query: 62  KAGGGGFMY--KQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQI 121
           K G   FMY  + +  LP S+DWR  GAVT +K+Q      CG CWAFS + AVEGIN+I
Sbjct: 113 KRGSKQFMYDHENLSKLPSSVDWRKKGAVTEIKNQG----KCGSCWAFSTVAAVEGINKI 172

Query: 122 RTKQLVPLSEQELVDCD-QMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSS 181
           +T +LV LSEQELVDCD + + GC GG ME AF+FI++NGGITTE +YPY    G C +S
Sbjct: 173 KTNKLVSLSEQELVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDAS 232

Query: 182 SSRMNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFL 241
                LVTIDG+++VP  +E+AL++AVANQPVSVA++    DFQFY              
Sbjct: 233 KDNGVLVTIDGHEDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSE------------ 292

Query: 242 GVFNGNCGVVLNHAVVAVGYEPD----YWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGI 301
           GVF G+CG  LNH V AVGY  +    YWI++NSWGA WGEGGY++++R  + P G CGI
Sbjct: 293 GVFTGSCGTELNHGVAAVGYGSERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGI 344

Query: 302 AIDASFPIK 304
           A++AS+PIK
Sbjct: 353 AMEASYPIK 344

[Close]

BLAST of ClCG08G003250 vs. ExPASy TrEMBL
Match: A0A1S3BA70 (vignain-like OS=Cucumis melo OX=3656 GN=LOC103487731 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 3.5e-94
Identity = 178/307 (57.98%), Postives = 218/307 (71.01%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  RF VF+EN  HV  VNQMNK YKLKLN+F DMSN EF N YA SNI+++R L   R
Sbjct: 56  EKHKRFSVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNFYARSNISHFRKL-HGR 115

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           + G GGFMY+Q  +LP S+DWR  GAV  +K Q      CG CWAFS++ AVE IN+I+T
Sbjct: 116 RRGAGGFMYEQDTDLPSSVDWRERGAVNAIKEQGT----CGSCWAFSSVAAVEAINKIKT 175

Query: 122 KQLVPLSEQELVDCDQMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSSR 181
            QL+ LSEQEL+DC+  + GC GG+ME AFDFI++NGGI TE +YPY+  +G C SS   
Sbjct: 176 NQLLSLSEQELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRIS 235

Query: 182 MNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGVF 241
             +V IDGY++VP  NEDALMQAVANQPVSVA++ +G DFQFY            + GVF
Sbjct: 236 SPIVKIDGYESVPE-NEDALMQAVANQPVSVAIDAAGRDFQFY------------WQGVF 295

Query: 242 NGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIAI 301
           +G CG  LNH VVA+GY       DYWI++NSWG GWGE GYVRM+RG   P GLCGIA+
Sbjct: 296 DGYCGTELNHGVVAIGYGTTEDGTDYWIVRNSWGVGWGEDGYVRMKRGVEQPEGLCGIAM 344

Query: 302 DASFPIK 304
           +AS+PIK
Sbjct: 356 EASYPIK 344

[Close]

BLAST of ClCG08G003250 vs. ExPASy TrEMBL
Match: A0A7N2L9B0 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 4.6e-94
Identity = 179/322 (55.59%), Postives = 224/322 (69.57%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  RF VF+EN KHVHKVN+MNK YKLKLN+F DM+N EF++ YA+S ++++R  +  R
Sbjct: 55  EKHKRFNVFKENVKHVHKVNKMNKPYKLKLNKFADMTNHEFSSSYASSKVSHHRMFQGIR 114

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           +    GFM++++ NLP S+DWR  GAVTG+K Q      CG CWAFS + AVEGINQI+T
Sbjct: 115 QV--TGFMHEKIDNLPTSVDWRKQGAVTGVKDQG----KCGSCWAFSTVVAVEGINQIKT 174

Query: 122 KQLVPLSEQELVDCDQMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSSR 181
           K+LVPLSEQELVDCD  + GC GG ME AF+FI+Q GG+TTE NYPY A+   C SS   
Sbjct: 175 KELVPLSEQELVDCDSKNNGCDGGLMENAFEFIKQTGGVTTEKNYPYQARHKSCDSSKMN 234

Query: 182 MNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGVF 241
             +V IDGY+NVP  +E+ALM+AVANQPVSVA++  G DFQFY              GVF
Sbjct: 235 DPMVQIDGYENVPQNDENALMKAVANQPVSVAIDAGGKDFQFYSE------------GVF 294

Query: 242 NGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIAI 301
            G+CG  LNH V  VGY        YWI+KNSWG  WGE GY+RMQRG N   GLCGIA+
Sbjct: 295 TGDCGTELNHGVAIVGYGTTLDGKKYWIVKNSWGPEWGEKGYIRMQRGVNAEEGLCGIAM 354

Query: 302 DASFPIK-----KKKIVSKEDI 314
           +AS+P+K     KKK   K+++
Sbjct: 355 EASYPVKLSSNNKKKTSFKDEL 358

[Close]

BLAST of ClCG08G003250 vs. ExPASy TrEMBL
Match: A0A0A0LMU4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G349680 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.7e-93
Identity = 177/307 (57.65%), Postives = 218/307 (71.01%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  RF VF+EN  HV  VNQM+K YKLKLN+F DMSN EF N YA SNI++YR L + R
Sbjct: 56  EKHKRFSVFKENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHE-R 115

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           + G GGFMY+Q  +LP S+DWR  GAV  +K Q      CG CWAFS++ AVEGIN+I+T
Sbjct: 116 RRGAGGFMYEQDTDLPSSVDWRERGAVNAVKEQG----RCGSCWAFSSVAAVEGINKIKT 175

Query: 122 KQLVPLSEQELVDCDQMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSSR 181
            QL+ LSEQEL+DC+  + GC GG+ME AFDFI++NGGI TE +YPY+  +G C SS   
Sbjct: 176 NQLLSLSEQELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRIS 235

Query: 182 MNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGVF 241
             +V IDGY++VP  NEDALMQAVANQPVSVA++ +G DFQFY              GVF
Sbjct: 236 SPIVKIDGYESVPE-NEDALMQAVANQPVSVAIDAAGRDFQFYS------------QGVF 295

Query: 242 NGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIAI 301
           +G CG  LNH VVA+GY       DYW+++NSWG GWGE GYVRM+RG     GLCGIA+
Sbjct: 296 DGYCGTELNHGVVAIGYGTTEDGTDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAM 344

Query: 302 DASFPIK 304
           +AS+PIK
Sbjct: 356 EASYPIK 344

[Close]

BLAST of ClCG08G003250 vs. ExPASy TrEMBL
Match: A0A6J1KIL0 (vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111496079 PE=3 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 3.9e-93
Identity = 176/307 (57.33%), Postives = 216/307 (70.36%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  R+ VF+ENA HV  VNQMNK YKLKLN+F DMSN EF NLYA SNI +YR L   R
Sbjct: 55  EKHKRYNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRR 114

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           + G  GFMY++  +LP  IDWR  GAV  +K Q      CG CWAFSA+ AVEGINQI+T
Sbjct: 115 REGASGFMYEKATDLPSFIDWRERGAVNDIKYQG----RCGSCWAFSAVAAVEGINQIKT 174

Query: 122 KQLVPLSEQELVDCDQMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSSR 181
            QL+ LSEQEL+DC+  + GC GG+META++FIR+NGGI +E NYPY   +G C SS   
Sbjct: 175 NQLLSLSEQELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMP 234

Query: 182 MNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGVF 241
             +VTIDG+++VP  NE+ALMQAVANQPVSV++E  G DFQFY            + GVF
Sbjct: 235 SPIVTIDGFESVPE-NENALMQAVANQPVSVSIEALGRDFQFY------------WQGVF 294

Query: 242 NGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIAI 301
           +G CG  LNH VV +GY       DYW ++NSWG GWGE GY+RM+RG   P GLCGIA+
Sbjct: 295 DGYCGTELNHGVVVIGYGTTDGGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAM 344

Query: 302 DASFPIK 304
           +AS+P+K
Sbjct: 355 EASYPLK 344

[Close]

BLAST of ClCG08G003250 vs. ExPASy TrEMBL
Match: A0A6J1GHN5 (vignain-like OS=Cucurbita moschata OX=3662 GN=LOC111454276 PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 5.1e-93
Identity = 174/307 (56.68%), Postives = 217/307 (70.68%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  R+ VF+ENA HV  VNQMNK YKLKLN+F DMSN EF N+YA SNI +YR L   R
Sbjct: 55  EKHKRYNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNVYARSNITHYRRLHGKR 114

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           + G  GFMY++  +LP  IDWR  GAV+ +K Q      CG CWAFSA+ AVEGINQI+T
Sbjct: 115 REGASGFMYEKATDLPSFIDWRERGAVSDIKDQG----RCGSCWAFSAVAAVEGINQIKT 174

Query: 122 KQLVPLSEQELVDCDQMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSSR 181
            QL+ LSEQEL+DC+  + GC GG+META++FIR+NGGI +E NYPY   +G C SS   
Sbjct: 175 NQLLSLSEQELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMP 234

Query: 182 MNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGVF 241
             +VTIDG+++VP  NE+ALMQAVANQPVSV++E  G DFQFY            + GVF
Sbjct: 235 SPIVTIDGFESVPE-NENALMQAVANQPVSVSIEALGRDFQFY------------WQGVF 294

Query: 242 NGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIAI 301
           +G+CG  LNH VV +GY       DYW ++NSWG GWGE GY+RM+RG   P GLCGI +
Sbjct: 295 DGDCGTELNHGVVVIGYGTTDGGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIVM 344

Query: 302 DASFPIK 304
           +AS+P+K
Sbjct: 355 EASYPLK 344

[Close]

BLAST of ClCG08G003250 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 315.8 bits (808), Expect = 4.5e-86
Identity = 162/308 (52.60%), Postives = 204/308 (66.23%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+  RF VF+ N KH+H+ N+ +KSYKLKLN+FGDM++ EF   YA SNI ++R  +  +
Sbjct: 53  EKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEK 112

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           KA    FMY  V  LP S+DWR  GAVT +K+Q      CG CWAFS + AVEGINQIRT
Sbjct: 113 KA-TKSFMYANVNTLPTSVDWRKNGAVTPVKNQG----QCGSCWAFSTVVAVEGINQIRT 172

Query: 122 KQLVPLSEQELVDCD-QMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSS 181
           K+L  LSEQELVDCD   + GC GG M+ AF+FI++ GG+T+E  YPY A    C ++  
Sbjct: 173 KKLTSLSEQELVDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKE 232

Query: 182 RMNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGV 241
              +V+IDG+++VP  +ED LM+AVANQPVSVA++  G DFQFY              GV
Sbjct: 233 NAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSE------------GV 292

Query: 242 FNGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIA 301
           F G CG  LNH V  VGY        YWI+KNSWG  WGE GY+RMQRG     GLCGIA
Sbjct: 293 FTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIA 343

Query: 302 IDASFPIK 304
           ++AS+P+K
Sbjct: 353 MEASYPLK 343

[Close]

BLAST of ClCG08G003250 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 315.5 bits (807), Expect = 5.9e-86
Identity = 163/309 (52.75%), Postives = 209/309 (67.64%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           ER  RF VF  N  HVH  N+ N+SYKLKLN+F D++ +EF N Y  SNI ++R L+   
Sbjct: 53  EREKRFNVFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGP- 112

Query: 62  KAGGGGFMY--KQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQI 121
           K G   FMY  + +  LP S+DWR  GAVT +K+Q      CG CWAFS + AVEGIN+I
Sbjct: 113 KRGSKQFMYDHENLSKLPSSVDWRKKGAVTEIKNQG----KCGSCWAFSTVAAVEGINKI 172

Query: 122 RTKQLVPLSEQELVDCD-QMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSS 181
           +T +LV LSEQELVDCD + + GC GG ME AF+FI++NGGITTE +YPY    G C +S
Sbjct: 173 KTNKLVSLSEQELVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDAS 232

Query: 182 SSRMNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFL 241
                LVTIDG+++VP  +E+AL++AVANQPVSVA++    DFQFY              
Sbjct: 233 KDNGVLVTIDGHEDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSE------------ 292

Query: 242 GVFNGNCGVVLNHAVVAVGYEPD----YWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGI 301
           GVF G+CG  LNH V AVGY  +    YWI++NSWGA WGEGGY++++R  + P G CGI
Sbjct: 293 GVFTGSCGTELNHGVAAVGYGSERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGI 344

Query: 302 AIDASFPIK 304
           A++AS+PIK
Sbjct: 353 AMEASYPIK 344

[Close]

BLAST of ClCG08G003250 vs. TAIR 10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 3.6e-83
Identity = 158/309 (51.13%), Postives = 204/309 (66.02%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E   RF VF  N  HVH+ N+ NK YKLK+NRF D+++ EF + YA SN+ ++R L+   
Sbjct: 53  EAIKRFNVFRHNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGP- 112

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           K G GGFMY+ V  +P S+DWR  GAVT +K+Q      CG CWAFS + AVEGIN+IRT
Sbjct: 113 KRGSGGFMYENVTRVPSSVDWREKGAVTEVKNQQ----DCGSCWAFSTVAAVEGINKIRT 172

Query: 122 KQLVPLSEQELVDCD-QMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQ-GYCTSSS 181
            +LV LSEQELVDCD + + GC GG ME AF+FI+ NGGI TE  YPY++    +C ++S
Sbjct: 173 NKLVSLSEQELVDCDTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANS 232

Query: 182 SRMNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLG 241
                VTIDG+++VP  +E+ L++AVA+QPVSVA++    DFQ Y              G
Sbjct: 233 IGGETVTIDGHEHVPENDEEELLKAVAHQPVSVAIDAGSSDFQLYSE------------G 292

Query: 242 VFNGNCGVVLNHAVVAVGY-----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGI 301
           VF G CG  LNH VV VGY        YWI++NSWG  WGEGGYVR++RG +   G CGI
Sbjct: 293 VFIGECGTQLNHGVVIVGYGETKNGTKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGI 344

Query: 302 AIDASFPIK 304
           A++AS+P K
Sbjct: 353 AMEASYPTK 344

[Close]

BLAST of ClCG08G003250 vs. TAIR 10
Match: AT1G20850.1 (xylem cysteine peptidase 2 )

HSP 1 Score: 271.6 bits (693), Expect = 9.8e-73
Identity = 142/309 (45.95%), Postives = 192/309 (62.14%), Query Frame = 0


Query: 2   ERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKTR 61
           E+F RF+VF++N KH+ + N+  KSY L LN F D+S+ EF  +Y     +  R   +  
Sbjct: 67  EKFLRFEVFKDNLKHIDETNKKGKSYWLGLNEFADLSHEEFKKMYLGLKTDIVR---RDE 126

Query: 62  KAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIRT 121
           +     F Y+ V  +P S+DWR  GAV  +K+Q      CG CWAFS + AVEGIN+I T
Sbjct: 127 ERSYAEFAYRDVEAVPKSVDWRKKGAVAEVKNQG----SCGSCWAFSTVAAVEGINKIVT 186

Query: 122 KQLVPLSEQELVDCD-QMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSSS 181
             L  LSEQEL+DCD   + GC GG M+ AF++I +NGG+  E +YPY+ ++G C     
Sbjct: 187 GNLTTLSEQELIDCDTTYNNGCNGGLMDYAFEYIVKNGGLRKEEDYPYSMEEGTCEMQKD 246

Query: 182 RMNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLGV 241
               VTI+G+Q+VP  +E +L++A+A+QP+SVA++ SG +FQFY G            GV
Sbjct: 247 ESETVTINGHQDVPTNDEKSLLKALAHQPLSVAIDASGREFQFYSG------------GV 306

Query: 242 FNGNCGVVLNHAVVAVGY----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIAI 301
           F+G CGV L+H V AVGY      DY I+KNSWG  WGE GY+R++R    P GLCGI  
Sbjct: 307 FDGRCGVDLDHGVAAVGYGSSKGSDYIIVKNSWGPKWGEKGYIRLKRNTGKPEGLCGINK 356

Query: 302 DASFPIKKK 306
            ASFP K K
Sbjct: 367 MASFPTKTK 356

[Close]

BLAST of ClCG08G003250 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 270.0 bits (689), Expect = 2.8e-72
Identity = 142/308 (46.10%), Postives = 190/308 (61.69%), Query Frame = 0


Query: 1   MERFNRFKVFEENAKHVHKVNQMNKSYKLKLNRFGDMSNSEFANLYANSNINYYRNLKKT 60
           +E+  RF++F++N + V + N+ N SY+L L RF D++N E+ + Y  + +      KK 
Sbjct: 67  VEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKME-----KKG 126

Query: 61  RKAGGGGFMYKQVLNLPPSIDWRAMGAVTGLKSQDLGAIGCGCCWAFSAIGAVEGINQIR 120
            +     +  +    LP SIDWR  GAV  +K Q     GCG CWAFS IGAVEGINQI 
Sbjct: 127 ERRTSLRYEARVGDELPESIDWRKKGAVAEVKDQG----GCGSCWAFSTIGAVEGINQIV 186

Query: 121 TKQLVPLSEQELVDCD-QMDGGCGGGYMETAFDFIRQNGGITTEANYPYNAKQGYCTSSS 180
           T  L+ LSEQELVDCD   + GC GG M+ AF+FI +NGGI T+ +YPY    G C    
Sbjct: 187 TGDLITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIR 246

Query: 181 SRMNLVTIDGYQNVPPYNEDALMQAVANQPVSVALEGSGLDFQFYGGASSSYSFFLCFLG 240
               +VTID Y++VP Y+E++L +AVA+QP+S+A+E  G  FQ Y              G
Sbjct: 247 KNAKVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDS------------G 306

Query: 241 VFNGNCGVVLNHAVVAVGY----EPDYWIMKNSWGAGWGEGGYVRMQRGANPPAGLCGIA 300
           +F+G+CG  L+H VVAVGY      DYWI++NSWG  WGE GY+RM R     +G CGIA
Sbjct: 307 IFDGSCGTQLDHGVVAVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIA 353

Query: 301 IDASFPIK 304
           I+ S+PIK
Sbjct: 367 IEPSYPIK 353

The following BLAST results are available for this feature:

BLAST of ClCG08G003250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038885064.1	2.7e-96	58.96	LOW QUALITY PROTEIN: vignain-like [Benincasa hispida]	[more]
XP_008444390.1	7.2e-94	57.98	PREDICTED: vignain-like [Cucumis melo]	[more]
XP_030957117.1	9.4e-94	55.59	vignain-like [Quercus lobata]	[more]
XP_023870610.1	2.7e-93	55.59	vignain-like [Quercus suber]	[more]
XP_004142960.1	3.6e-93	57.65	vignain [Cucumis sativus] >KGN62334.1 hypothetical protein Csa_018585 [Cucumis s...	[more]

BLAST of ClCG08G003250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P25803	3.1e-87	53.57	Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2	[more]
O65039	5.2e-87	53.72	Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1	[more]
P12412	2.0e-86	53.25	Vignain OS=Vigna mungo OX=3915 PE=1 SV=1	[more]
Q9FGR9	6.4e-85	52.60	KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 ...	[more]
Q9STL4	8.3e-85	52.75	KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ...	[more]

BLAST of ClCG08G003250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BA70	3.5e-94	57.98	vignain-like OS=Cucumis melo OX=3656 GN=LOC103487731 PE=3 SV=1	[more]
A0A7N2L9B0	4.6e-94	55.59	Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1	[more]
A0A0A0LMU4	1.7e-93	57.65	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G349680 PE=3 SV=1	[more]
A0A6J1KIL0	3.9e-93	57.33	vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111496079 PE=3 SV=1	[more]
A0A6J1GHN5	5.1e-93	56.68	vignain-like OS=Cucurbita moschata OX=3662 GN=LOC111454276 PE=3 SV=1	[more]

BLAST of ClCG08G003250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50260.1	4.5e-86	52.60	Cysteine proteinases superfamily protein	[more]
AT3G48340.1	5.9e-86	52.75	Cysteine proteinases superfamily protein	[more]
AT3G48350.1	3.6e-83	51.13	Cysteine proteinases superfamily protein	[more]
AT1G20850.1	9.8e-73	45.95	xylem cysteine peptidase 2	[more]
AT1G47128.1	2.8e-72	46.10	Granulin repeat cysteine protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 262..268 score: 71.26 coord: 98..113 score: 50.74 coord: 251..261 score: 48.85
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 76..302 e-value: 3.9E-88 score: 308.8
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 76..302 e-value: 1.9E-63 score: 214.5
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 1..42 e-value: 9.8E-4 score: 28.4
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 5..42 e-value: 1.6E-7 score: 31.7
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 1..304 e-value: 4.7E-98 score: 330.9
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 2..324
None	No IPR available	PANTHER	PTHR12411:SF749	CYSTEINE PROTEASE	coord: 2..324
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 262..281
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 77..301 e-value: 3.37636E-90 score: 268.338
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 2..302

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG08G003250.1	ClCG08G003250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity