ClCG08G001965 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG08G001965
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	Heat shock 70 kDa protein
Location	CG_Chr08: 4079068 .. 4079609 (+)
RNA-Seq Expression	ClCG08G001965
Synteny	ClCG08G001965

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTCAAGAAATAGAGAAGTACAAATTTGAGGATGAGGAGAACAAGAAAAAGGTGGAGGCCAAGAATTGTTTCGAGAACTACGCTTACAACATGAGGAACACTGTGAACAGTGAGAAAATTTGGCCATGCTTTCCCAGGTGGACACGAAGAAGACCGACGATTCCATTGAGCACTTTAGGAGAAATGAGGGTTTCTAGTCTTTGTTGTATGAGAATGGAGAGAAAGCCAAAGAAATGTGCACAAAGTTGTGTAATGTTTTGGCGTGAGGTTAATGGGTTTGGAGAAAAATCTGAGCGCACCATTTTTTCTTCATTTACCGCTTTTTTTTTTCTTTTTATTAATAGCCTATTCCACAAGAGGCTATATTAAAAGGCTATAAAAAGGGCTATATCATGTAGTGCCTGAGACATTACAATTGGTATCAGAATGGAACCTATCACAGTAAGATGTGGTATCAGAATAGAACCTATCACAGTAAGATGTGGTTTAGAGACAAACCAAGTGGAAGCTGGTGGACATGTAATGCAAGACTTGAATGA

mRNA sequence

ATGGTTCAAGAAATAGAGAAGTACAAATTTGAGGATGAGGAGAACAAGAAAAAGGTGGAGGCCAAGAATTGTTTCGAGAACTACGCTTACAACATGAGGAACACTGTGAACAGTGAGAAAATTTGGCCATGCTTTCCCAGGTGGACACGAAGAAGACCGACGATTCCATTGAGCACTTTAGGAGAAATGAGGGTTTCTAGTCTTTGTTGTATGAGAATGGAGAGAAAGCCAAAGAAATGTGCACAAAGTTGTGTAATGTTTTGGCGTGAGAATGGAACCTATCACAGTAAGATGTGGTATCAGAATAGAACCTATCACAGTAAGATGTGGTTTAGAGACAAACCAAGTGGAAGCTGGTGGACATGTAATGCAAGACTTGAATGA

Coding sequence (CDS)

ATGGTTCAAGAAATAGAGAAGTACAAATTTGAGGATGAGGAGAACAAGAAAAAGGTGGAGGCCAAGAATTGTTTCGAGAACTACGCTTACAACATGAGGAACACTGTGAACAGTGAGAAAATTTGGCCATGCTTTCCCAGGTGGACACGAAGAAGACCGACGATTCCATTGAGCACTTTAGGAGAAATGAGGGTTTCTAGTCTTTGTTGTATGAGAATGGAGAGAAAGCCAAAGAAATGTGCACAAAGTTGTGTAATGTTTTGGCGTGAGAATGGAACCTATCACAGTAAGATGTGGTATCAGAATAGAACCTATCACAGTAAGATGTGGTTTAGAGACAAACCAAGTGGAAGCTGGTGGACATGTAATGCAAGACTTGAATGA

Protein sequence

MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKIWPCFPRWTRRRPTIPLSTLGEMRVSSLCCMRMERKPKKCAQSCVMFWRENGTYHSKMWYQNRTYHSKMWFRDKPSGSWWTCNARLE

Homology

[Close]

BLAST of ClCG08G001965 vs. NCBI nr
Match: KAF9611928.1 (hypothetical protein IFM89_036744 [Coptis chinensis])

HSP 1 Score: 70.1 bits (170), Expect = 1.6e-08
Identity = 37/63 (58.73%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKIWPCFPRWTRRRPTIPLSTL 60
           MVQE EKYK EDEE+KKKVE+KN FENY+YNMRNT+  EK      +  RRR  +PL  L
Sbjct: 130 MVQEAEKYKSEDEEHKKKVESKNAFENYSYNMRNTIKDEKNASNLTQQIRRRLKMPLRLL 189

Query: 61  GEM 64
             +
Sbjct: 190 SRL 192

[Close]

BLAST of ClCG08G001965 vs. NCBI nr
Match: KAF3527059.1 (hypothetical protein F2Q69_00046373 [Brassica cretica])

HSP 1 Score: 69.3 bits (168), Expect = 2.7e-08
Identity = 34/41 (82.93%), Postives = 36/41 (87.80%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKI 42
           MVQE EKYKFEDEE+KKKVEAKN  ENYAYNMRNT+  EKI
Sbjct: 490 MVQEAEKYKFEDEEHKKKVEAKNALENYAYNMRNTIRDEKI 530

[Close]

BLAST of ClCG08G001965 vs. NCBI nr
Match: XP_022962967.1 (heat shock cognate 70 kDa protein 2 [Cucurbita moschata] >KAG6595311.1 Heat shock cognate 70 kDa protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 68.9 bits (167), Expect = 3.5e-08
Identity = 35/41 (85.37%), Postives = 36/41 (87.80%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKI 42
           MVQE EKYK EDEE+KKKVEAKN  ENYAYNMRNTVN EKI
Sbjct: 524 MVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTVNDEKI 564

[Close]

BLAST of ClCG08G001965 vs. NCBI nr
Match: KGN54238.1 (hypothetical protein Csa_017889 [Cucumis sativus])

HSP 1 Score: 68.9 bits (167), Expect = 3.5e-08
Identity = 35/41 (85.37%), Postives = 36/41 (87.80%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKI 42
           MVQE EKYK EDEE+KKKVEAKN  ENYAYNMRNTVN EKI
Sbjct: 524 MVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTVNDEKI 564

[Close]

BLAST of ClCG08G001965 vs. NCBI nr
Match: AZJ17860.1 (heat shock protein 70-1 [Cucurbita moschata])

HSP 1 Score: 68.9 bits (167), Expect = 3.5e-08
Identity = 35/41 (85.37%), Postives = 36/41 (87.80%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKI 42
           MVQE EKYK EDEE+KKKVEAKN  ENYAYNMRNTVN EKI
Sbjct: 524 MVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTVNDEKI 564

[Close]

BLAST of ClCG08G001965 vs. ExPASy Swiss-Prot
Match: P22953 (Heat shock 70 kDa protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP70-1 PE=1 SV=3)

HSP 1 Score: 66.2 bits (160), Expect = 3.0e-10
Identity = 34/52 (65.38%), Postives = 39/52 (75.00%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKIWPCFPRWTRRR 53
           MVQE EKYK EDEE+KKKVEAKN  ENYAYNMRNT+  EKI    P   +++
Sbjct: 524 MVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIQDEKIGEKLPAADKKK 575

[Close]

BLAST of ClCG08G001965 vs. ExPASy Swiss-Prot
Match: P22954 (Heat shock 70 kDa protein 2 OS=Arabidopsis thaliana OX=3702 GN=HSP70-2 PE=1 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 3.0e-10
Identity = 34/52 (65.38%), Postives = 39/52 (75.00%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKIWPCFPRWTRRR 53
           MVQE EKYK EDEE+KKKVEAKN  ENYAYNMRNT+  EKI    P   +++
Sbjct: 524 MVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIRDEKIGEKLPAADKKK 575

[Close]

BLAST of ClCG08G001965 vs. ExPASy Swiss-Prot
Match: P27322 (Heat shock cognate 70 kDa protein 2 OS=Solanum lycopersicum OX=4081 GN=HSC-2 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 3.0e-10
Identity = 33/41 (80.49%), Postives = 35/41 (85.37%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKI 42
           MVQE EKYK EDEE+KKKVEAKN  ENYAYNMRNT+  EKI
Sbjct: 524 MVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIKDEKI 564

[Close]

BLAST of ClCG08G001965 vs. ExPASy Swiss-Prot
Match: Q9LHA8 (Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 6.6e-10
Identity = 32/41 (78.05%), Postives = 35/41 (85.37%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKI 42
           MVQE EKYK EDEE+KKKV+AKN  ENYAYNMRNT+  EKI
Sbjct: 524 MVQEAEKYKAEDEEHKKKVDAKNALENYAYNMRNTIKDEKI 564

[Close]

BLAST of ClCG08G001965 vs. ExPASy Swiss-Prot
Match: O65719 (Heat shock 70 kDa protein 3 OS=Arabidopsis thaliana OX=3702 GN=HSP70-3 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 6.6e-10
Identity = 32/41 (78.05%), Postives = 35/41 (85.37%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKI 42
           MVQE EKYK EDEE+KKKV+AKN  ENYAYNMRNT+  EKI
Sbjct: 524 MVQEAEKYKSEDEEHKKKVDAKNALENYAYNMRNTIRDEKI 564

[Close]

BLAST of ClCG08G001965 vs. ExPASy TrEMBL
Match: A0A6J1B7E5 (heat shock cognate 70 kDa protein 2 isoform X1 OS=Herrania umbratica OX=108875 GN=LOC110424890 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.7e-08
Identity = 35/41 (85.37%), Postives = 36/41 (87.80%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKI 42
           MVQE EKYK EDEE+KKKVEAKN  ENYAYNMRNTVN EKI
Sbjct: 524 MVQEAEKYKAEDEEHKKKVEAKNALENYAYNMRNTVNDEKI 564

[Close]

BLAST of ClCG08G001965 vs. ExPASy TrEMBL
Match: A0A5A7SRF3 (Heat shock cognate 70 kDa protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G00110 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.7e-08
Identity = 35/41 (85.37%), Postives = 36/41 (87.80%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKI 42
           MVQE EKYK EDEE+KKKVEAKN  ENYAYNMRNTVN EKI
Sbjct: 524 MVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTVNDEKI 564

[Close]

BLAST of ClCG08G001965 vs. ExPASy TrEMBL
Match: A0A6J1IC54 (heat shock cognate 70 kDa protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111471170 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.7e-08
Identity = 35/41 (85.37%), Postives = 36/41 (87.80%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKI 42
           MVQE EKYK EDEE+KKKVEAKN  ENYAYNMRNTVN EKI
Sbjct: 524 MVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTVNDEKI 564

[Close]

BLAST of ClCG08G001965 vs. ExPASy TrEMBL
Match: A0A6J1ILN4 (heat shock cognate 70 kDa protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111478145 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.7e-08
Identity = 35/41 (85.37%), Postives = 36/41 (87.80%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKI 42
           MVQE EKYK EDEE+KKKVEAKN  ENYAYNMRNTVN EKI
Sbjct: 524 MVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTVNDEKI 564

[Close]

BLAST of ClCG08G001965 vs. ExPASy TrEMBL
Match: Q8GSN4 (Non-cell-autonomous heat shock cognate protein 70 OS=Cucurbita maxima OX=3661 GN=Hsc70-2 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.7e-08
Identity = 35/41 (85.37%), Postives = 36/41 (87.80%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKI 42
           MVQE EKYK EDEE+KKKVEAKN  ENYAYNMRNTVN EKI
Sbjct: 524 MVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTVNDEKI 564

[Close]

BLAST of ClCG08G001965 vs. TAIR 10
Match: AT5G02490.1 (Heat shock protein 70 (Hsp 70) family protein )

HSP 1 Score: 66.2 bits (160), Expect = 2.1e-11
Identity = 34/52 (65.38%), Postives = 39/52 (75.00%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKIWPCFPRWTRRR 53
           MVQE EKYK EDEE+KKKVEAKN  ENYAYNMRNT+  EKI    P   +++
Sbjct: 524 MVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIRDEKIGEKLPAADKKK 575

[Close]

BLAST of ClCG08G001965 vs. TAIR 10
Match: AT5G02500.1 (heat shock cognate protein 70-1 )

HSP 1 Score: 66.2 bits (160), Expect = 2.1e-11
Identity = 34/52 (65.38%), Postives = 39/52 (75.00%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKIWPCFPRWTRRR 53
           MVQE EKYK EDEE+KKKVEAKN  ENYAYNMRNT+  EKI    P   +++
Sbjct: 524 MVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIQDEKIGEKLPAADKKK 575

[Close]

BLAST of ClCG08G001965 vs. TAIR 10
Match: AT5G02500.2 (heat shock cognate protein 70-1 )

HSP 1 Score: 66.2 bits (160), Expect = 2.1e-11
Identity = 34/52 (65.38%), Postives = 39/52 (75.00%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKIWPCFPRWTRRR 53
           MVQE EKYK EDEE+KKKVEAKN  ENYAYNMRNT+  EKI    P   +++
Sbjct: 394 MVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIQDEKIGEKLPAADKKK 445

[Close]

BLAST of ClCG08G001965 vs. TAIR 10
Match: AT3G12580.1 (heat shock protein 70 )

HSP 1 Score: 65.1 bits (157), Expect = 4.7e-11
Identity = 32/41 (78.05%), Postives = 35/41 (85.37%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKI 42
           MVQE EKYK EDEE+KKKV+AKN  ENYAYNMRNT+  EKI
Sbjct: 524 MVQEAEKYKAEDEEHKKKVDAKNALENYAYNMRNTIKDEKI 564

[Close]

BLAST of ClCG08G001965 vs. TAIR 10
Match: AT3G09440.1 (Heat shock protein 70 (Hsp 70) family protein )

HSP 1 Score: 65.1 bits (157), Expect = 4.7e-11
Identity = 32/41 (78.05%), Postives = 35/41 (85.37%), Query Frame = 0


Query: 1   MVQEIEKYKFEDEENKKKVEAKNCFENYAYNMRNTVNSEKI 42
           MVQE EKYK EDEE+KKKV+AKN  ENYAYNMRNT+  EKI
Sbjct: 524 MVQEAEKYKSEDEEHKKKVDAKNALENYAYNMRNTIRDEKI 564

The following BLAST results are available for this feature:

BLAST of ClCG08G001965 vs. NCBI nr


Match Name	E-value	Identity	Description
KAF9611928.1	1.6e-08	58.73	hypothetical protein IFM89_036744 [Coptis chinensis]	[more]
KAF3527059.1	2.7e-08	82.93	hypothetical protein F2Q69_00046373 [Brassica cretica]	[more]
XP_022962967.1	3.5e-08	85.37	heat shock cognate 70 kDa protein 2 [Cucurbita moschata] >KAG6595311.1 Heat shoc...	[more]
KGN54238.1	3.5e-08	85.37	hypothetical protein Csa_017889 [Cucumis sativus]	[more]
AZJ17860.1	3.5e-08	85.37	heat shock protein 70-1 [Cucurbita moschata]	[more]

BLAST of ClCG08G001965 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P22953	3.0e-10	65.38	Heat shock 70 kDa protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP70-1 PE=1 SV=3	[more]
P22954	3.0e-10	65.38	Heat shock 70 kDa protein 2 OS=Arabidopsis thaliana OX=3702 GN=HSP70-2 PE=1 SV=2	[more]
P27322	3.0e-10	80.49	Heat shock cognate 70 kDa protein 2 OS=Solanum lycopersicum OX=4081 GN=HSC-2 PE=...	[more]
Q9LHA8	6.6e-10	78.05	Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1	[more]
O65719	6.6e-10	78.05	Heat shock 70 kDa protein 3 OS=Arabidopsis thaliana OX=3702 GN=HSP70-3 PE=1 SV=1	[more]

BLAST of ClCG08G001965 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1B7E5	1.7e-08	85.37	heat shock cognate 70 kDa protein 2 isoform X1 OS=Herrania umbratica OX=108875 G...	[more]
A0A5A7SRF3	1.7e-08	85.37	Heat shock cognate 70 kDa protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A6J1IC54	1.7e-08	85.37	heat shock cognate 70 kDa protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1ILN4	1.7e-08	85.37	heat shock cognate 70 kDa protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111478145 ...	[more]
Q8GSN4	1.7e-08	85.37	Non-cell-autonomous heat shock cognate protein 70 OS=Cucurbita maxima OX=3661 GN...	[more]

BLAST of ClCG08G001965 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G02490.1	2.1e-11	65.38	Heat shock protein 70 (Hsp 70) family protein	[more]
AT5G02500.1	2.1e-11	65.38	heat shock cognate protein 70-1	[more]
AT5G02500.2	2.1e-11	65.38	heat shock cognate protein 70-1	[more]
AT3G12580.1	4.7e-11	78.05	heat shock protein 70	[more]
AT3G09440.1	4.7e-11	78.05	Heat shock protein 70 (Hsp 70) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029048	Heat shock protein 70kD, C-terminal domain superfamily	GENE3D	1.20.1270.10		coord: 1..51 e-value: 1.0E-13 score: 53.3
None	No IPR available	PANTHER	PTHR19375:SF424	HEAT SHOCK COGNATE 70 KDA PROTEIN 2-LIKE	coord: 1..46
IPR013126	Heat shock protein 70 family	PANTHER	PTHR19375	HEAT SHOCK PROTEIN 70KDA	coord: 1..46

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG08G001965.1	ClCG08G001965.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034620	cellular response to unfolded protein
biological_process	GO:0051085	chaperone cofactor-dependent protein refolding
biological_process	GO:0042026	protein refolding
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0031072	heat shock protein binding
molecular_function	GO:0051787	misfolded protein binding
molecular_function	GO:0044183	protein folding chaperone
molecular_function	GO:0051082	unfolded protein binding