ClCG08G000170 (gene) Watermelon (Charleston Gray) v2.5

BLAST of ClCG08G000170 vs. NCBI nr
Match: XP_038890567.1 (hydrolase tropI [Benincasa hispida])

HSP 1 Score: 526.6 bits (1355), Expect = 1.5e-145
Identity = 264/314 (84.08%), Postives = 281/314 (89.49%), Query Frame = 0

Query: 1   MGLGSAGAQLISVPATGSSS--RHMGTPFQPPCFRIAASPLPFRKCRNKDIWKWKGKR-- 60
           MGL SAGAQLIS+P TGSSS  R +GTPFQ P FR   SPLPFRKC N+  WKWKGKR  
Sbjct: 1   MGLASAGAQLISLPPTGSSSCRRRIGTPFQLPYFRF-TSPLPFRKCGNRSDWKWKGKREK 60

Query: 61  --GKVCYSVEGVKDNEIDDEGCELVSGMELSIGEQLHHSIPAYLFKAVKNNNGTAILLLS 120
             GKVCYSVEGV+D++ DD  CELV+GMELSIGEQ HHSIPAYLFKAVKNNNGTAILLLS
Sbjct: 61  KGGKVCYSVEGVEDDDDDDAACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLS 120

Query: 121 DLFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDPWEKQPEQLSAKREMQRIAEDIGIITK 180
           DLFAFQHSSIRDFAYRLACNGFNVLLPDLIHGD W+KQ    S KR+MQRIAEDIG +TK
Sbjct: 121 DLFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDSWKKQ----STKRDMQRIAEDIGTVTK 180

Query: 181 WLVDEFSAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSVASKVKV 240
           WLVDEFSAAG+SRKLGIMGFGYGGG+VI+VLA+DEGACF IGASLYGTEVDPS+A+KVKV
Sbjct: 181 WLVDEFSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTEVDPSLATKVKV 240

Query: 241 PVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDEDAEQAFLM 300
           PVLLISGDNDPLCPVSVMKDLEK MGEGSKLVIF GRGH FVHNP SPEEDEDAEQAFLM
Sbjct: 241 PVLLISGDNDPLCPVSVMKDLEKRMGEGSKLVIFGGRGHDFVHNPRSPEEDEDAEQAFLM 300

Query: 301 LRNWLHHGLVATNI 309
           +RNWLHHGLVATNI
Sbjct: 301 IRNWLHHGLVATNI 309

BLAST of ClCG08G000170 vs. NCBI nr
Match: XP_004141569.1 (uncharacterized protein LOC101210518 [Cucumis sativus] >KGN52723.1 hypothetical protein Csa_008898 [Cucumis sativus])

HSP 1 Score: 498.8 bits (1283), Expect = 3.3e-137
Identity = 244/307 (79.48%), Postives = 269/307 (87.62%), Query Frame = 0

Query: 1   MGLGSAGAQLISVPATGSSSRHMGTPFQPPCFRIAASPLPFRKCRNKDIWKWKGKRGKVC 60
           MG  SAGAQLIS+P TGSSSR +G PFQPP F+  A PLP RK  N  +W+WK   GK+ 
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKF-AYPLPSRKYSNMGVWRWK---GKLR 60

Query: 61  YSVEGVKDNEIDDEGCELVSGMELSIGEQLHHSIPAYLFKAVKNNNGTAILLLSDLFAFQ 120
           YSVEG   ++ DD+ CELV+GMELSIGEQ HHSIPAYLFKAVKNNNGTAILLLSDLFAFQ
Sbjct: 61  YSVEGRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQ 120

Query: 121 HSSIRDFAYRLACNGFNVLLPDLIHGDPWEKQPEQLSAKREMQRIAEDIGIITKWLVDEF 180
           HSSIRDFAYRLAC GFNVLLPDL+H D WEK P +  AK+EMQRIAEDIG +TKWL +EF
Sbjct: 121 HSSIRDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEF 180

Query: 181 SAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPVLLIS 240
           SAAG+S KLGI+GFGYGGGRVIDVLA+DEG CF IGAS+YGT+VDPS+A+KVKVPVLLIS
Sbjct: 181 SAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLIS 240

Query: 241 GDNDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDEDAEQAFLMLRNWLH 300
           GDNDPLCPVSVMKDLEKG+GEGSKLVIF GRGHGFVHNPGSPEED+DAE+AFLMLRNWLH
Sbjct: 241 GDNDPLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLH 300

Query: 301 HGLVATN 308
           HGLVA N
Sbjct: 301 HGLVAIN 303

BLAST of ClCG08G000170 vs. NCBI nr
Match: XP_008459689.1 (PREDICTED: uncharacterized protein LOC103498731 [Cucumis melo] >KAA0039216.1 carboxymethylenebutenolidase-like protein [Cucumis melo var. makuwa] >TYK00404.1 carboxymethylenebutenolidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 494.6 bits (1272), Expect = 6.3e-136
Identity = 251/308 (81.49%), Postives = 271/308 (87.99%), Query Frame = 0

Query: 1   MGLGSAGAQLISVPATGSSSRHMGTPFQPPCFRIAASPLPFRKCRNKDIWKWKGKRGKVC 60
           MG  SAGAQLIS+P TGSSSR +  P QPP +   A PLP  K  N   WKWKGK     
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPP-YSQFAYPLPSWKYNNWGDWKWKGKLR--Y 60

Query: 61  YSVEG-VKDNEIDDEGCELVSGMELSIGEQLHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120
           YSV+G VKD+  DD+ CELV+GMELSIGEQ HHSIPAYLFKAVKNNNGTAILLLSDLFAF
Sbjct: 61  YSVKGRVKDD--DDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120

Query: 121 QHSSIRDFAYRLACNGFNVLLPDLIHGDPWEKQPEQLSAKREMQRIAEDIGIITKWLVDE 180
           Q SSIRDFAYRLACNGFNVLLPDL++ DPWEK P Q SAK+EMQRIAEDIG +TKWL DE
Sbjct: 121 QDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDE 180

Query: 181 FSAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPVLLI 240
           FSAAG+SRKLGI+GFG+GGGRVIDVLA+D+GACF IGASLYGTEVDPS+A+KVKVPVLLI
Sbjct: 181 FSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLI 240

Query: 241 SGDNDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDEDAEQAFLMLRNWL 300
           SGD+DPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEED+DAEQAFLMLRNWL
Sbjct: 241 SGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWL 300

Query: 301 HHGLVATN 308
           HHGLVATN
Sbjct: 301 HHGLVATN 303

BLAST of ClCG08G000170 vs. NCBI nr
Match: XP_022925419.1 (carboxymethylenebutenolidase homolog [Cucurbita moschata] >KAG6592400.1 Carboxymethylenebutenolidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 445.7 bits (1145), Expect = 3.3e-121
Identity = 233/312 (74.68%), Postives = 252/312 (80.77%), Query Frame = 0

Query: 1   MGLGSAGAQLISVPATGSSSRHMGTPFQPPCFRIAASPLPFRKCRNKDIWKWKGKR---- 60
           MG  +AGAQ IS P TG S R MG PFQ P FR A    P     +  I + KGKR    
Sbjct: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQ-PSFRFA---FPI----SNSIGQLKGKREIKG 60

Query: 61  GKVCYSVEGVKDNEIDDEGCELVSGMELSIGEQLHHSIPAYLFKAVKNNNGTAILLLSDL 120
           GKVCYS EGVKD   ++E CELV+GMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+DL
Sbjct: 61  GKVCYSAEGVKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDL 120

Query: 121 FAFQHSSIRDFAYRLACNGFNVLLPDLIHGDPWEKQPEQLSAKREMQRIAEDIGIITKWL 180
           FAFQ SS+RDFAYRLACNGFNVLLPD+ HGD  E+           Q+  EDI   TKWL
Sbjct: 121 FAFQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEE-----------QKTVEDIAAATKWL 180

Query: 181 VDEFSAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPV 240
           VDEFSAAGISRKLGI+GFGYGGGRVIDVLAQD+G+ FSIGASLYGTEV+PS+A+KV+VPV
Sbjct: 181 VDEFSAAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPV 240

Query: 241 LLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDEDAEQAFLMLR 300
           LLISGDNDPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PGSPEEDEDAEQAFLMLR
Sbjct: 241 LLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLR 293

Query: 301 NWLHHGLVATNI 309
           NWLH GLVATNI
Sbjct: 301 NWLHDGLVATNI 293

BLAST of ClCG08G000170 vs. NCBI nr
Match: XP_022973982.1 (carboxymethylenebutenolidase homolog [Cucurbita maxima])

HSP 1 Score: 444.1 bits (1141), Expect = 9.7e-121
Identity = 233/312 (74.68%), Postives = 253/312 (81.09%), Query Frame = 0

Query: 1   MGLGSAGAQLISVPATGSSSRHMGTPFQPPCFRIAASPLPFRKCRNKDIWKWKGKR---- 60
           MG  SAGAQ IS P TG S R MG PFQ P FR  A P+      + +I + KGKR    
Sbjct: 1   MGSASAGAQWISAPPTGRSGRRMGIPFQ-PSFR-CAFPI------SNNIGQLKGKREKKG 60

Query: 61  GKVCYSVEGVKDNEIDDEGCELVSGMELSIGEQLHHSIPAYLFKAVKNNNGTAILLLSDL 120
           GKVCYS EGVKD    +E CELVSGMELSIGE+ HHSI A+LFKAVKNNNGTAILLL+DL
Sbjct: 61  GKVCYSAEGVKDEIDAEEACELVSGMELSIGEEAHHSITAHLFKAVKNNNGTAILLLTDL 120

Query: 121 FAFQHSSIRDFAYRLACNGFNVLLPDLIHGDPWEKQPEQLSAKREMQRIAEDIGIITKWL 180
           F+FQ SS+RDFAYRLACNGFNVLLPD+ HGD  E+           Q+  EDIG  TKWL
Sbjct: 121 FSFQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEE-----------QKAVEDIGAATKWL 180

Query: 181 VDEFSAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPV 240
           VDEF AAGISRKLGI+GFGYGGGRVIDVLAQD+G+ FSIGASLYGTEV+PS+A+KV+VPV
Sbjct: 181 VDEFLAAGISRKLGIVGFGYGGGRVIDVLAQDQGSYFSIGASLYGTEVNPSLATKVEVPV 240

Query: 241 LLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDEDAEQAFLMLR 300
           LLISGDNDPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PGSPEEDEDAEQAFLMLR
Sbjct: 241 LLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLR 293

Query: 301 NWLHHGLVATNI 309
           NWLH GLVATNI
Sbjct: 301 NWLHDGLVATNI 293

BLAST of ClCG08G000170 vs. ExPASy Swiss-Prot
Match: Q96DG6 (Carboxymethylenebutenolidase homolog OS=Homo sapiens OX=9606 GN=CMBL PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 5.2e-16
Identity = 68/226 (30.09%), Postives = 111/226 (49.12%), Query Frame = 0

Query: 89  QLHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHG-D 148
           Q+ H I AY+ K+   + G A++++ D+F +Q  + R  A  ++ NG+  ++PD   G +
Sbjct: 26  QVEH-IKAYVTKS-PVDAGKAVIVIQDIFGWQLPNTRYIADMISGNGYTTIVPDFFVGQE 85

Query: 149 PWEKQ------PEQLSAKREMQRIAEDIGIITKWLVDEFSAAGISRKLGIMGFGYGGGRV 208
           PW+        PE L   R  Q+I  +I  I K+L  +  A    +K+GI+GF +GG  V
Sbjct: 86  PWDPSGDWSIFPEWLKT-RNAQKIDREISAILKYLKQQCHA----QKIGIVGFCWGGTAV 145

Query: 209 IDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGE 268
             ++ +     F  G S+YG   D      +K P L I  +ND + P+  +  L + + E
Sbjct: 146 HHLMMKYSE--FRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKE 205

Query: 269 GSK----LVIFEGRGHGFVHNPG---SPEEDEDAEQAFLMLRNWLH 301
             K    +  F G+ HGFVH      SP +    ++A   L  WL+
Sbjct: 206 HCKVEYQIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLN 242

BLAST of ClCG08G000170 vs. ExPASy Swiss-Prot
Match: Q5RBU3 (Carboxymethylenebutenolidase homolog OS=Pongo abelii OX=9601 GN=CMBL PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 8.8e-16
Identity = 68/226 (30.09%), Postives = 111/226 (49.12%), Query Frame = 0

Query: 89  QLHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHG-D 148
           Q+ H I AY+ K+   + G A++++ D+F +Q  + R  A  ++ NG+  ++PD   G +
Sbjct: 26  QVEH-IKAYVTKS-PVDAGKAVIVIQDIFGWQLPNTRYMADMISGNGYTTIVPDFFVGQE 85

Query: 149 PWEKQ------PEQLSAKREMQRIAEDIGIITKWLVDEFSAAGISRKLGIMGFGYGGGRV 208
           PW+        PE L   R  Q+I  +I  I K+L  +  A    +K+GI+GF +GG  V
Sbjct: 86  PWDPSGDWSIFPEWLKT-RNAQKIDREISAILKYLKQQCHA----QKIGIVGFCWGGIAV 145

Query: 209 IDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGE 268
             ++ +     F  G S+YG   D      +K P L I  +ND + P+  +  L + + E
Sbjct: 146 HHLMMKYSE--FRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKE 205

Query: 269 GSK----LVIFEGRGHGFVHNPG---SPEEDEDAEQAFLMLRNWLH 301
             K    +  F G+ HGFVH      SP +    ++A   L  WL+
Sbjct: 206 HCKVEYQIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLN 242

BLAST of ClCG08G000170 vs. ExPASy Swiss-Prot
Match: Q7TP52 (Carboxymethylenebutenolidase homolog OS=Rattus norvegicus OX=10116 GN=Cmbl PE=2 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.8e-14
Identity = 66/226 (29.20%), Postives = 110/226 (48.67%), Query Frame = 0

Query: 89  QLHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHG-- 148
           Q+ H I AY+ ++   + G A++++ D+F +Q S+ R  A  +A NG+  ++PD   G  
Sbjct: 26  QVEH-IKAYVTRS-PVDAGKAVIVVQDIFGWQLSNTRYMADMIAGNGYTTIVPDFFVGQE 85

Query: 149 --DP---WEKQPEQLSAKREMQRIAEDIGIITKWLVDEFSAAGISRKLGIMGFGYGGGRV 208
             DP   W   PE L + R  ++I  ++  + ++L  +  A    +K+GI+GF +GG  V
Sbjct: 86  PWDPAGDWSTFPEWLKS-RNARKINREVDAVLRYLKQQCHA----QKIGIVGFCWGGIVV 145

Query: 209 IDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGE 268
             V+          G S+YG   D      +K P L I  +ND + P+  +  L + + E
Sbjct: 146 HHVMTTYPEV--RAGVSVYGIIRDSEDVYNLKNPTLFIFAENDAVIPLEQVSILIQKLKE 205

Query: 269 ----GSKLVIFEGRGHGFVHNPG---SPEEDEDAEQAFLMLRNWLH 301
                 ++  F G+ HGFVH      SP +    E+A   L  WL+
Sbjct: 206 HCIVNYQVKTFSGQTHGFVHRKREDCSPADKPYIEEARRNLIEWLN 242

BLAST of ClCG08G000170 vs. ExPASy Swiss-Prot
Match: Q8R1G2 (Carboxymethylenebutenolidase homolog OS=Mus musculus OX=10090 GN=Cmbl PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.4e-13
Identity = 65/249 (26.10%), Postives = 116/249 (46.59%), Query Frame = 0

Query: 69  NEIDDEGCELVSGMELSIGEQLH----HSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSI 128
           NE +   C++  G +L  G   H      I AY+ ++   + G A++++ D+F +Q  + 
Sbjct: 3   NEANPCPCDI--GHKLEYGGMGHEVQVEHIKAYVTRS-PVDAGKAVIVVQDIFGWQLPNT 62

Query: 129 RDFAYRLACNGFNVLLPDLIHG-DPWEKQPEQLS-----AKREMQRIAEDIGIITKWLVD 188
           R  A  +A NG+  ++PD   G +PW+   +  +       R  +++  ++  + ++L  
Sbjct: 63  RYMADMIARNGYTTIVPDFFVGQEPWDPAGDWSTFPAWLKSRNARKVNREVDAVLRYLRQ 122

Query: 189 EFSAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPVLL 248
           +  A    +K+GI+GF +GG  V  V+          G S+YG   D      +K P L 
Sbjct: 123 QCHA----QKIGIVGFCWGGVVVHQVMTAYPD--IRAGVSVYGIIRDSEDVYNLKNPTLF 182

Query: 249 ISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFEGRGHGFVHNPG---SPEEDEDAEQA 301
           I  +ND + P+  +  L + + E      ++  F G+ HGFVH      SP +    E+A
Sbjct: 183 IFAENDTVIPLEQVSTLTQKLKEHCIVNYQVKTFSGQTHGFVHRKREDCSPADKPYIEEA 242

BLAST of ClCG08G000170 vs. ExPASy Swiss-Prot
Match: B8M9K0 (Hydrolase tropI OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=tropI PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 2.2e-06
Identity = 41/179 (22.91%), Postives = 84/179 (46.93%), Query Frame = 0

Query: 110 ILLLSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHGD--PWEKQPEQLSAK-------- 169
           +L+L+D+   + S+ +  A + A NG+ V++PDL  GD  P  K  E    K        
Sbjct: 46  VLILTDIVGHRFSNAQIIADQFAENGYFVMMPDLFLGDAVPLNKPGEFDMGKWRSGAYHP 105

Query: 170 REMQRIAEDIGIITKWLVDEFSAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASL 229
           +    + E +  I +  + E  +    +K+G +G+ +GG  V+  L   +     +G + 
Sbjct: 106 QGKNHLPETVDPIVEVCLSEMRSKYQCKKIGAVGYCFGGKYVVRHLIPGK---MDVGYTA 165

Query: 230 YGTEVDPSVASKVKVPVLLISGDNDPLCPVS---VMKDLEKGMGEGSKLVIFEGRGHGF 276
           + + +D S    +K P+ + +   D + P     V +++ + +G   ++ ++ G  HGF
Sbjct: 166 HPSHIDESELKGIKGPLAIAAAAKDNIFPAEKRHVSEEILQEVGFPYQINLYSGVSHGF 221

BLAST of ClCG08G000170 vs. ExPASy TrEMBL
Match: A0A0A0KT04 (DLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652280 PE=4 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.6e-137
Identity = 244/307 (79.48%), Postives = 269/307 (87.62%), Query Frame = 0

Query: 1   MGLGSAGAQLISVPATGSSSRHMGTPFQPPCFRIAASPLPFRKCRNKDIWKWKGKRGKVC 60
           MG  SAGAQLIS+P TGSSSR +G PFQPP F+  A PLP RK  N  +W+WK   GK+ 
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKF-AYPLPSRKYSNMGVWRWK---GKLR 60

Query: 61  YSVEGVKDNEIDDEGCELVSGMELSIGEQLHHSIPAYLFKAVKNNNGTAILLLSDLFAFQ 120
           YSVEG   ++ DD+ CELV+GMELSIGEQ HHSIPAYLFKAVKNNNGTAILLLSDLFAFQ
Sbjct: 61  YSVEGRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQ 120

Query: 121 HSSIRDFAYRLACNGFNVLLPDLIHGDPWEKQPEQLSAKREMQRIAEDIGIITKWLVDEF 180
           HSSIRDFAYRLAC GFNVLLPDL+H D WEK P +  AK+EMQRIAEDIG +TKWL +EF
Sbjct: 121 HSSIRDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEF 180

Query: 181 SAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPVLLIS 240
           SAAG+S KLGI+GFGYGGGRVIDVLA+DEG CF IGAS+YGT+VDPS+A+KVKVPVLLIS
Sbjct: 181 SAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLIS 240

Query: 241 GDNDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDEDAEQAFLMLRNWLH 300
           GDNDPLCPVSVMKDLEKG+GEGSKLVIF GRGHGFVHNPGSPEED+DAE+AFLMLRNWLH
Sbjct: 241 GDNDPLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLH 300

Query: 301 HGLVATN 308
           HGLVA N
Sbjct: 301 HGLVAIN 303

BLAST of ClCG08G000170 vs. ExPASy TrEMBL
Match: A0A5A7TB03 (Carboxymethylenebutenolidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00030 PE=4 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 3.0e-136
Identity = 251/308 (81.49%), Postives = 271/308 (87.99%), Query Frame = 0

Query: 1   MGLGSAGAQLISVPATGSSSRHMGTPFQPPCFRIAASPLPFRKCRNKDIWKWKGKRGKVC 60
           MG  SAGAQLIS+P TGSSSR +  P QPP +   A PLP  K  N   WKWKGK     
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPP-YSQFAYPLPSWKYNNWGDWKWKGKLR--Y 60

Query: 61  YSVEG-VKDNEIDDEGCELVSGMELSIGEQLHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120
           YSV+G VKD+  DD+ CELV+GMELSIGEQ HHSIPAYLFKAVKNNNGTAILLLSDLFAF
Sbjct: 61  YSVKGRVKDD--DDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120

Query: 121 QHSSIRDFAYRLACNGFNVLLPDLIHGDPWEKQPEQLSAKREMQRIAEDIGIITKWLVDE 180
           Q SSIRDFAYRLACNGFNVLLPDL++ DPWEK P Q SAK+EMQRIAEDIG +TKWL DE
Sbjct: 121 QDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDE 180

Query: 181 FSAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPVLLI 240
           FSAAG+SRKLGI+GFG+GGGRVIDVLA+D+GACF IGASLYGTEVDPS+A+KVKVPVLLI
Sbjct: 181 FSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLI 240

Query: 241 SGDNDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDEDAEQAFLMLRNWL 300
           SGD+DPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEED+DAEQAFLMLRNWL
Sbjct: 241 SGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWL 300

Query: 301 HHGLVATN 308
           HHGLVATN
Sbjct: 301 HHGLVATN 303

BLAST of ClCG08G000170 vs. ExPASy TrEMBL
Match: A0A1S3CB89 (uncharacterized protein LOC103498731 OS=Cucumis melo OX=3656 GN=LOC103498731 PE=4 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 3.0e-136
Identity = 251/308 (81.49%), Postives = 271/308 (87.99%), Query Frame = 0

Query: 1   MGLGSAGAQLISVPATGSSSRHMGTPFQPPCFRIAASPLPFRKCRNKDIWKWKGKRGKVC 60
           MG  SAGAQLIS+P TGSSSR +  P QPP +   A PLP  K  N   WKWKGK     
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPP-YSQFAYPLPSWKYNNWGDWKWKGKLR--Y 60

Query: 61  YSVEG-VKDNEIDDEGCELVSGMELSIGEQLHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120
           YSV+G VKD+  DD+ CELV+GMELSIGEQ HHSIPAYLFKAVKNNNGTAILLLSDLFAF
Sbjct: 61  YSVKGRVKDD--DDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAF 120

Query: 121 QHSSIRDFAYRLACNGFNVLLPDLIHGDPWEKQPEQLSAKREMQRIAEDIGIITKWLVDE 180
           Q SSIRDFAYRLACNGFNVLLPDL++ DPWEK P Q SAK+EMQRIAEDIG +TKWL DE
Sbjct: 121 QDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDE 180

Query: 181 FSAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPVLLI 240
           FSAAG+SRKLGI+GFG+GGGRVIDVLA+D+GACF IGASLYGTEVDPS+A+KVKVPVLLI
Sbjct: 181 FSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLI 240

Query: 241 SGDNDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDEDAEQAFLMLRNWL 300
           SGD+DPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEED+DAEQAFLMLRNWL
Sbjct: 241 SGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWL 300

Query: 301 HHGLVATN 308
           HHGLVATN
Sbjct: 301 HHGLVATN 303

BLAST of ClCG08G000170 vs. ExPASy TrEMBL
Match: A0A6J1EC40 (carboxymethylenebutenolidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111432716 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.6e-121
Identity = 233/312 (74.68%), Postives = 252/312 (80.77%), Query Frame = 0

Query: 1   MGLGSAGAQLISVPATGSSSRHMGTPFQPPCFRIAASPLPFRKCRNKDIWKWKGKR---- 60
           MG  +AGAQ IS P TG S R MG PFQ P FR A    P     +  I + KGKR    
Sbjct: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQ-PSFRFA---FPI----SNSIGQLKGKREIKG 60

Query: 61  GKVCYSVEGVKDNEIDDEGCELVSGMELSIGEQLHHSIPAYLFKAVKNNNGTAILLLSDL 120
           GKVCYS EGVKD   ++E CELV+GMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+DL
Sbjct: 61  GKVCYSAEGVKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDL 120

Query: 121 FAFQHSSIRDFAYRLACNGFNVLLPDLIHGDPWEKQPEQLSAKREMQRIAEDIGIITKWL 180
           FAFQ SS+RDFAYRLACNGFNVLLPD+ HGD  E+           Q+  EDI   TKWL
Sbjct: 121 FAFQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEE-----------QKTVEDIAAATKWL 180

Query: 181 VDEFSAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPV 240
           VDEFSAAGISRKLGI+GFGYGGGRVIDVLAQD+G+ FSIGASLYGTEV+PS+A+KV+VPV
Sbjct: 181 VDEFSAAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPV 240

Query: 241 LLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDEDAEQAFLMLR 300
           LLISGDNDPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PGSPEEDEDAEQAFLMLR
Sbjct: 241 LLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLR 293

Query: 301 NWLHHGLVATNI 309
           NWLH GLVATNI
Sbjct: 301 NWLHDGLVATNI 293

BLAST of ClCG08G000170 vs. ExPASy TrEMBL
Match: A0A6J1I908 (carboxymethylenebutenolidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111472595 PE=4 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 4.7e-121
Identity = 233/312 (74.68%), Postives = 253/312 (81.09%), Query Frame = 0

Query: 1   MGLGSAGAQLISVPATGSSSRHMGTPFQPPCFRIAASPLPFRKCRNKDIWKWKGKR---- 60
           MG  SAGAQ IS P TG S R MG PFQ P FR  A P+      + +I + KGKR    
Sbjct: 1   MGSASAGAQWISAPPTGRSGRRMGIPFQ-PSFR-CAFPI------SNNIGQLKGKREKKG 60

Query: 61  GKVCYSVEGVKDNEIDDEGCELVSGMELSIGEQLHHSIPAYLFKAVKNNNGTAILLLSDL 120
           GKVCYS EGVKD    +E CELVSGMELSIGE+ HHSI A+LFKAVKNNNGTAILLL+DL
Sbjct: 61  GKVCYSAEGVKDEIDAEEACELVSGMELSIGEEAHHSITAHLFKAVKNNNGTAILLLTDL 120

Query: 121 FAFQHSSIRDFAYRLACNGFNVLLPDLIHGDPWEKQPEQLSAKREMQRIAEDIGIITKWL 180
           F+FQ SS+RDFAYRLACNGFNVLLPD+ HGD  E+           Q+  EDIG  TKWL
Sbjct: 121 FSFQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEE-----------QKAVEDIGAATKWL 180

Query: 181 VDEFSAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPV 240
           VDEF AAGISRKLGI+GFGYGGGRVIDVLAQD+G+ FSIGASLYGTEV+PS+A+KV+VPV
Sbjct: 181 VDEFLAAGISRKLGIVGFGYGGGRVIDVLAQDQGSYFSIGASLYGTEVNPSLATKVEVPV 240

Query: 241 LLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDEDAEQAFLMLR 300
           LLISGDNDPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PGSPEEDEDAEQAFLMLR
Sbjct: 241 LLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLR 293

Query: 301 NWLHHGLVATNI 309
           NWLH GLVATNI
Sbjct: 301 NWLHDGLVATNI 293

BLAST of ClCG08G000170 vs. TAIR 10
Match: AT1G35420.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 297.4 bits (760), Expect = 1.4e-80
Identity = 151/299 (50.50%), Postives = 199/299 (66.56%), Query Frame = 0

Query: 20  SRHMGTPFQPPCFRIAASPLPFRKCRNKDIWKW--KGKRGKVCYSVE---GVKDNEIDD- 79
           +RH      PP     AS + F + +      +  +G   K  Y++E   G  + E+DD 
Sbjct: 22  NRHGSHRLPPPQIIPLASSIMFCQIKKHITTSFYRRGSSLKNIYALETSDGAINVEVDDD 81

Query: 80  ---EGCELVSGMELSIGEQLHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDFAYR 139
              E CELV+G E+S+       +  YL  AVKNNNGT +LLLSD+F FQ S+ RDFAYR
Sbjct: 82  EEEEACELVNGTEVSV-----DGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDFAYR 141

Query: 140 LACNGFNVLLPDLIHGDPWEK-----QPEQLSAKREMQRIAEDIGIITKWLVDEFSAAGI 199
           +ACNG+NVL+PDL  GDPW K     + E+     +  RI +D    TKW+V+EF+AAGI
Sbjct: 142 VACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAAAGI 201

Query: 200 SRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPVLLISGDNDP 259
           S+KLG+MGF +GGGRV+DVLA DE   FS G S YGT +D +VA  VKVPVL I+GD DP
Sbjct: 202 SKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGDRDP 261

Query: 260 LCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDEDAEQAFLMLRNWLHHGLV 305
           LC V  + ++E+ +GEGSK+V++EGRGHGFVH P +PE+D DAE+AF ++RNWLHH L+
Sbjct: 262 LCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMRNWLHHHLI 315

BLAST of ClCG08G000170 vs. TAIR 10
Match: AT1G35420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 297.0 bits (759), Expect = 1.8e-80
Identity = 148/297 (49.83%), Postives = 199/297 (67.00%), Query Frame = 0

Query: 13  VPATGSSSRHMGTPFQPPCFRIAASPLPFRKCRNKDIWKWKGKRGKVCYSVEGVKDNEID 72
           +P   S  +H+ T F    +R  +S         K+I+  +   G +   V    D++ +
Sbjct: 35  IPLASSIKKHITTSF----YRRGSS--------LKNIYALETSDGAINVEV----DDDEE 94

Query: 73  DEGCELVSGMELSIGEQLHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSSIRDFAYRLA 132
           +E CELV+G E+S+       +  YL  AVKNNNGT +LLLSD+F FQ S+ RDFAYR+A
Sbjct: 95  EEACELVNGTEVSV-----DGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDFAYRVA 154

Query: 133 CNGFNVLLPDLIHGDPWEK-----QPEQLSAKREMQRIAEDIGIITKWLVDEFSAAGISR 192
           CNG+NVL+PDL  GDPW K     + E+     +  RI +D    TKW+V+EF+AAGIS+
Sbjct: 155 CNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAAAGISK 214

Query: 193 KLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSVASKVKVPVLLISGDNDPLC 252
           KLG+MGF +GGGRV+DVLA DE   FS G S YGT +D +VA  VKVPVL I+GD DPLC
Sbjct: 215 KLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGDRDPLC 274

Query: 253 PVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDEDAEQAFLMLRNWLHHGLV 305
            V  + ++E+ +GEGSK+V++EGRGHGFVH P +PE+D DAE+AF ++RNWLHH L+
Sbjct: 275 EVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMRNWLHHHLI 310

BLAST of ClCG08G000170 vs. TAIR 10
Match: AT3G23600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 2.8e-09
Identity = 48/196 (24.49%), Postives = 94/196 (47.96%), Query Frame = 0

Query: 110 ILLLSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDPWEKQPEQLSAKREMQRIAEDI 169
           +LL+SD+F F+  ++R  A ++A +GF V++PD   GDP+    +       ++    D 
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDK 101

Query: 170 GI-ITKWLVDEFSAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSV 229
           G   TK +++     GI+  +G  G  + G +V+  L+++E         L+ + V+   
Sbjct: 102 GFENTKPVLETIKNKGIT-AIGAAGMCW-GAKVVVELSKEE--LIQAAVLLHPSFVNVDD 161

Query: 230 ASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFEGRGHGFV--HNPGSP 289
               K P+ ++  + D + P +++K  E+ +       S + I     HG+   +N   P
Sbjct: 162 IKGGKAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEP 221

Query: 290 EEDEDAEQAFLMLRNW 299
           E  + AE+A   + +W
Sbjct: 222 EAVKAAEEAHKEMLDW 233

BLAST of ClCG08G000170 vs. TAIR 10
Match: AT3G23570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 4.1e-08
Identity = 50/202 (24.75%), Postives = 96/202 (47.52%), Query Frame = 0

Query: 109 AILLLSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDPW----EKQPEQLSAK-REMQ 168
           A+LL+  +F ++  ++R  A ++A  GF  ++PD  HGDP+    + +P  +  K  E++
Sbjct: 41  AVLLVPHVFGYETPNLRKLADKVAEAGFYAVVPDFFHGDPYNPENQDRPFPIWMKDHELE 100

Query: 169 RIAEDIGIITKWLVDEFSAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTE 228
           +  E+    +K +V+     GI+  +G  GF + G +V   LA+++         L+   
Sbjct: 101 KGFEE----SKPIVEALKNKGIT-SIGAAGFCW-GAKVAVELAKEK--LVDATVLLHPAR 160

Query: 229 VDPSVASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFEGRGHGFV--H 288
           V      +V +P+ ++  + D + P  +++  E  +       S + IF    HG+   +
Sbjct: 161 VTVDDIKEVNLPIAVLGAEIDQVSPPELVRQFEDILASKPQVKSFVKIFPRCKHGWTVRY 220

Query: 289 NPGSPEEDEDAEQAFLMLRNWL 300
           N   P E E A +A   +  WL
Sbjct: 221 NENDPSEVEAAMEAHKDMLAWL 234

BLAST of ClCG08G000170 vs. TAIR 10
Match: AT3G23600.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 5.3e-08
Identity = 46/196 (23.47%), Postives = 89/196 (45.41%), Query Frame = 0

Query: 110 ILLLSDLFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDPWEKQPEQLSAKREMQRIAEDI 169
           +LL+SD+F F+  ++R  A ++A +GF V++PD   GDP+    +       ++    D 
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDK 101

Query: 170 GI-ITKWLVDEFSAAGISRKLGIMGFGYGGGRVIDVLAQDEGACFSIGASLYGTEVDPSV 229
           G   TK +++     GI+  +G  G  +G      V+   +         L+ + V+   
Sbjct: 102 GFENTKPVLETIKNKGIT-AIGAAGMCWG------VVELSKEELIQAAVLLHPSFVNVDD 161

Query: 230 ASKVKVPVLLISGDNDPLCPVSVMKDLEKGMGE----GSKLVIFEGRGHGFV--HNPGSP 289
               K P+ ++  + D + P +++K  E+ +       S + I     HG+   +N   P
Sbjct: 162 IKGGKAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEP 221

Query: 290 EEDEDAEQAFLMLRNW 299
           E  + AE+A   + +W
Sbjct: 222 EAVKAAEEAHKEMLDW 230