ClCG07G010460 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG07G010460
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Descriptiongibberellin 2-beta-dioxygenase 1
LocationCG_Chr07: 26197965 .. 26199548 (+)
RNA-Seq ExpressionClCG07G010460
SyntenyClCG07G010460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGTTTTGTCCAAACCATCAATCCAACAGTTGTATTCTAATTCCTTCATGAGAAATTCCATGTCATCATCCATGGCTGCTGCTGCTTCTTCCTCTGCAGCATTTTTCTCTGAGGTTCCATTCATAGACCTTTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAGCTTGGATTTTTCAAGGTCGTCAACCATGGCGTCCCCATGGAATTCATTTCTACTCTCGAATCAGAATCCACCAATTTCTTCTCCCTTCCACTCTCTGAAAAACAAAAAGCTGCTCCTCCTTCCCCTTTTGGCTATGGCAACAAGCAAATTGGTCGCAATGGCGACGTCGGTTGGGTCGAATATCTTCTCTTAAACACTCACCTAGAATCCAACTCCGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGGTAACGCAAAAACGCTCTGTTTTTTCTTCACTCTGTTTCTTGTGTTTTTCTCTGTTTTTTTCTGCTTCTGATTTCTGTTATCTGTTTCTATGTTCTAGCTCTGCTGTGAACAATTACATCTCGGCTGTGAGGAATATGGCGTGTGAAATCCTTGAGTTAATGGCGGAAGGATTGAAGATTCAACAGAGGAATGTATTCAGTAAGCTGGTGATGGATGAAGAGAGTGACTCTGTTTTCAGGGTGAACCATTATCCGCCATGTCCAGAGCTTCAAGCTTTGAAAGAGAGGAACATGATTGGATTTGGAGAACACACAGACCCACAAATCATATCGGTTTTGAGATCCAACAATACTTCTGGACTACAAATTTCTCTTGCTGATGGGAATTGGATTTCTGTTCCTCCTGATCAGAATTCCTTTTTCATCAATGTTGGTGACTCTTTACAGGTACAATAACATCACTGTTTCCATTCCAACCAAAATTTTTTAATTAAAAATAAGAATTTGTGTATGTTTCTTTCTCTCTCTTGGTTTCTCAATCTTTGTACTATATATATATATATATATATATTTTTAATCTCTATAGACAAGACATGATCTTTAATAATCATGATTATGTCTGTCAGCTGGGTTTGTGTTTAGATAAATCTCCCCAAGCCACTCATGTTGGTTCTTTCTTTGTTCTTTGATAATCAAAAGTATAAAAATTTGAGGTTACTTCCCATTTAGATTTTTCAAATACCCTACTTCAAAACTTGGGTTTTTCTTTTACTCCCATTTTGCAAAAAAGAAAAAGGGTCTGCAAAATACATGTGTGTGTGTGTTTTTTTTTCTTTTCTTTTTTTTTTTTTTTTAATATTATGTAGTAAAATTGAAATTTTGTTTGATGAAGGTGATGACTAATGGAAGATTCAAAAGTGTAAAGCATAGGGTTTTGACAAATAGCTTGAAGTCAAGAATTTCAATGATATACTTTGGTGGGCCACCTTTGAGTGAAAAGATAGCTCCTTTGCCTTCTCTTATGAAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACTTGGTTTGAGTATAAAAGATCAGCTTACAACTCCAGGTTGGCAGATAACAGGCTTGTGCACTTTGAAAGAATTGCAGCCTCATAA

mRNA sequence

ATGGTGGTTTTGTCCAAACCATCAATCCAACAGTTGTATTCTAATTCCTTCATGAGAAATTCCATGTCATCATCCATGGCTGCTGCTGCTTCTTCCTCTGCAGCATTTTTCTCTGAGGTTCCATTCATAGACCTTTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAGCTTGGATTTTTCAAGGTCGTCAACCATGGCGTCCCCATGGAATTCATTTCTACTCTCGAATCAGAATCCACCAATTTCTTCTCCCTTCCACTCTCTGAAAAACAAAAAGCTGCTCCTCCTTCCCCTTTTGGCTATGGCAACAAGCAAATTGGTCGCAATGGCGACGTCGGTTGGGTCGAATATCTTCTCTTAAACACTCACCTAGAATCCAACTCCGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGCTCTGCTGTGAACAATTACATCTCGGCTGTGAGGAATATGGCGTGTGAAATCCTTGAGTTAATGGCGGAAGGATTGAAGATTCAACAGAGGAATGTATTCAGTAAGCTGGTGATGGATGAAGAGAGTGACTCTGTTTTCAGGGTGAACCATTATCCGCCATGTCCAGAGCTTCAAGCTTTGAAAGAGAGGAACATGATTGGATTTGGAGAACACACAGACCCACAAATCATATCGGTTTTGAGATCCAACAATACTTCTGGACTACAAATTTCTCTTGCTGATGGGAATTGGATTTCTGTTCCTCCTGATCAGAATTCCTTTTTCATCAATGTTGGTGACTCTTTACAGGTGATGACTAATGGAAGATTCAAAAGTGTAAAGCATAGGGTTTTGACAAATAGCTTGAAGTCAAGAATTTCAATGATATACTTTGGTGGGCCACCTTTGAGTGAAAAGATAGCTCCTTTGCCTTCTCTTATGAAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACTTGGTTTGAGTATAAAAGATCAGCTTACAACTCCAGGTTGGCAGATAACAGGCTTGTGCACTTTGAAAGAATTGCAGCCTCATAA

Coding sequence (CDS)

ATGGTGGTTTTGTCCAAACCATCAATCCAACAGTTGTATTCTAATTCCTTCATGAGAAATTCCATGTCATCATCCATGGCTGCTGCTGCTTCTTCCTCTGCAGCATTTTTCTCTGAGGTTCCATTCATAGACCTTTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAGCTTGGATTTTTCAAGGTCGTCAACCATGGCGTCCCCATGGAATTCATTTCTACTCTCGAATCAGAATCCACCAATTTCTTCTCCCTTCCACTCTCTGAAAAACAAAAAGCTGCTCCTCCTTCCCCTTTTGGCTATGGCAACAAGCAAATTGGTCGCAATGGCGACGTCGGTTGGGTCGAATATCTTCTCTTAAACACTCACCTAGAATCCAACTCCGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGCTCTGCTGTGAACAATTACATCTCGGCTGTGAGGAATATGGCGTGTGAAATCCTTGAGTTAATGGCGGAAGGATTGAAGATTCAACAGAGGAATGTATTCAGTAAGCTGGTGATGGATGAAGAGAGTGACTCTGTTTTCAGGGTGAACCATTATCCGCCATGTCCAGAGCTTCAAGCTTTGAAAGAGAGGAACATGATTGGATTTGGAGAACACACAGACCCACAAATCATATCGGTTTTGAGATCCAACAATACTTCTGGACTACAAATTTCTCTTGCTGATGGGAATTGGATTTCTGTTCCTCCTGATCAGAATTCCTTTTTCATCAATGTTGGTGACTCTTTACAGGTGATGACTAATGGAAGATTCAAAAGTGTAAAGCATAGGGTTTTGACAAATAGCTTGAAGTCAAGAATTTCAATGATATACTTTGGTGGGCCACCTTTGAGTGAAAAGATAGCTCCTTTGCCTTCTCTTATGAAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACTTGGTTTGAGTATAAAAGATCAGCTTACAACTCCAGGTTGGCAGATAACAGGCTTGTGCACTTTGAAAGAATTGCAGCCTCATAA

Protein sequence

MVVLSKPSIQQLYSNSFMRNSMSSSMAAAASSSAAFFSEVPFIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQALKERNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Homology
BLAST of ClCG07G010460 vs. NCBI nr
Match: XP_038879745.1 (gibberellin 2-beta-dioxygenase 1-like [Benincasa hispida])

HSP 1 Score: 661.8 bits (1706), Expect = 3.3e-186
Identity = 338/346 (97.69%), Postives = 340/346 (98.27%), Query Frame = 0

Query: 1   MVVLSKPSIQQLYSNSFMRNSMSSSMAAAASSSAAFFSEVPFIDLSAPDAKQLIVKACEE 60
           MVVLS PSIQQL SNSFMRNS+ SSMAAAASSSAAFF+EVP IDLSAPDAKQLIVKACEE
Sbjct: 1   MVVLSNPSIQQLSSNSFMRNSI-SSMAAAASSSAAFFTEVPLIDLSAPDAKQLIVKACEE 60

Query: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120
           LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE
Sbjct: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120

Query: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180
           YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF
Sbjct: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180

Query: 181 SKLVMDEESDSVFRVNHYPPCPELQALKERNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240
           SKLVMDEESDSVFRVNHYPPCPE QALK RNMIGFGEHTDPQIISVLRSNNTSGLQISLA
Sbjct: 181 SKLVMDEESDSVFRVNHYPPCPEPQALKGRNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240

Query: 241 DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI 300
           DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI
Sbjct: 241 DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI 300

Query: 301 APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 347
           APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 345

BLAST of ClCG07G010460 vs. NCBI nr
Match: XP_008451787.1 (PREDICTED: gibberellin 2-beta-dioxygenase 1 [Cucumis melo])

HSP 1 Score: 652.1 bits (1681), Expect = 2.6e-183
Identity = 331/346 (95.66%), Postives = 336/346 (97.11%), Query Frame = 0

Query: 1   MVVLSKPSIQQLYSNSFMRNSMSSSMAAAASSSAAFFSEVPFIDLSAPDAKQLIVKACEE 60
           MVVLSK SIQQL SNSFMRN    SMA+AASSSAAFFSEVP IDLS+PDAKQLIVKACEE
Sbjct: 1   MVVLSKQSIQQLSSNSFMRN----SMASAASSSAAFFSEVPLIDLSSPDAKQLIVKACEE 60

Query: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120
           LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE
Sbjct: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120

Query: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180
           Y+LLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRN F
Sbjct: 121 YILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNAF 180

Query: 181 SKLVMDEESDSVFRVNHYPPCPELQALKERNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240
           SKLVMDEESDSVFRVNHYPPCP++QALK  NMIGFGEHTDPQIISVLRSNNTSGLQISLA
Sbjct: 181 SKLVMDEESDSVFRVNHYPPCPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240

Query: 241 DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI 300
           DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI
Sbjct: 241 DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI 300

Query: 301 APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 347
           APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 342

BLAST of ClCG07G010460 vs. NCBI nr
Match: KGN53539.1 (hypothetical protein Csa_014892 [Cucumis sativus])

HSP 1 Score: 649.0 bits (1673), Expect = 2.2e-182
Identity = 329/346 (95.09%), Postives = 335/346 (96.82%), Query Frame = 0

Query: 1   MVVLSKPSIQQLYSNSFMRNSMSSSMAAAASSSAAFFSEVPFIDLSAPDAKQLIVKACEE 60
           MV+LSK SIQQL SNSFMRN    SMA+AASSS AF SEVP IDLS+PDAKQLIVKACEE
Sbjct: 1   MVILSKQSIQQLSSNSFMRN----SMASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEE 60

Query: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120
           LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE
Sbjct: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120

Query: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180
           Y+LLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF
Sbjct: 121 YILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180

Query: 181 SKLVMDEESDSVFRVNHYPPCPELQALKERNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240
           SKLVMDEESDSVFRVNHYPPCP++QALK  NMIGFGEHTDPQIISVLRSNNTSGLQISLA
Sbjct: 181 SKLVMDEESDSVFRVNHYPPCPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240

Query: 241 DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI 300
           DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI
Sbjct: 241 DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI 300

Query: 301 APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 347
           APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 342

BLAST of ClCG07G010460 vs. NCBI nr
Match: KAA0063004.1 (gibberellin 2-beta-dioxygenase 1 [Cucumis melo var. makuwa] >TYK16342.1 gibberellin 2-beta-dioxygenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 627.1 bits (1616), Expect = 9.1e-176
Identity = 312/321 (97.20%), Postives = 317/321 (98.75%), Query Frame = 0

Query: 26  MAAAASSSAAFFSEVPFIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 85
           MA+AASSSAAFFSEVP IDLS+PDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN
Sbjct: 1   MASAASSSAAFFSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60

Query: 86  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 145
           FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEY+LLNTHLESNSDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120

Query: 146 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQ 205
           LRSAVNNYISAVRNMACEILELMAEGLKIQQRN FSKLVMDEESDSVFRVNHYPPCP++Q
Sbjct: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNAFSKLVMDEESDSVFRVNHYPPCPQIQ 180

Query: 206 ALKERNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 265
           ALK  NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM
Sbjct: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240

Query: 266 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 325
           TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300

Query: 326 SAYNSRLADNRLVHFERIAAS 347
           SAYNSRLADNRLVHFERIAAS
Sbjct: 301 SAYNSRLADNRLVHFERIAAS 321

BLAST of ClCG07G010460 vs. NCBI nr
Match: NP_001292640.1 (gibberellin 2-beta-dioxygenase 1 [Cucumis sativus] >CCD28485.1 GA 2-oxidase [Cucumis sativus])

HSP 1 Score: 624.4 bits (1609), Expect = 5.9e-175
Identity = 311/321 (96.88%), Postives = 316/321 (98.44%), Query Frame = 0

Query: 26  MAAAASSSAAFFSEVPFIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 85
           MA+AASSS AF SEVP IDLS+PDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN
Sbjct: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60

Query: 86  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 145
           FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEY+LLNTHLESNSDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120

Query: 146 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQ 205
           LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCP++Q
Sbjct: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180

Query: 206 ALKERNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 265
           ALK  NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM
Sbjct: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240

Query: 266 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 325
           TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300

Query: 326 SAYNSRLADNRLVHFERIAAS 347
           SAYNSRLADNRLVHFERIAAS
Sbjct: 301 SAYNSRLADNRLVHFERIAAS 321

BLAST of ClCG07G010460 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 3.9e-129
Identity = 233/346 (67.34%), Postives = 277/346 (80.06%), Query Frame = 0

Query: 1   MVVLSKPSIQQLYSNSFMRNSMSSSMAAAASSSAAFFSEVPFIDLSAPDAKQLIVKACEE 60
           MV+LSKP+ +Q    +++RN+M             F S +P +DLS PDAK LIVKACE+
Sbjct: 1   MVLLSKPTSEQY---TYVRNNM----------PITFSSSIPLVDLSKPDAKTLIVKACED 60

Query: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120
            GFFKV+NHG+P++ IS LESE+  FFSLP +EK+KA P +PFGYGNK+IG NGD+GW+E
Sbjct: 61  FGFFKVINHGIPLDAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIE 120

Query: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180
           YLLL T    N D   S++G+D  K R  + +Y  A+RNMACEIL+LMAEGLKIQ +NVF
Sbjct: 121 YLLLTT----NQDHNFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVF 180

Query: 181 SKLVMDEESDSVFRVNHYPPCPELQALKERNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240
           SKLVMD++SD +FRVNHYP CPEL A+   N+IGFGEHTDPQIIS+LRSNNTSG QISL 
Sbjct: 181 SKLVMDKQSDCLFRVNHYPACPEL-AINGENLIGFGEHTDPQIISILRSNNTSGFQISLR 240

Query: 241 DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI 300
           DG+WISVPPD +SFFINVGDSLQVMTNGRFKSV+HRVL N +  R+SMIYF GPPLSEKI
Sbjct: 241 DGSWISVPPDHSSFFINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKI 300

Query: 301 APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 347
           APLPSLMKG+E SLYKEFTWFEYK S Y SRLADNRL ++ERIAA+
Sbjct: 301 APLPSLMKGKE-SLYKEFTWFEYKSSTYGSRLADNRLGNYERIAAT 327

BLAST of ClCG07G010460 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.1e-120
Identity = 216/345 (62.61%), Postives = 261/345 (75.65%), Query Frame = 0

Query: 1   MVVLSKPSIQQLYSNSFMRNSMSSSMAAAASSSAAFFSEVPFIDLSAPDAKQLIVKACEE 60
           MVVLS+P++ Q +                   S   F+ +P +DL+ PDAK LIV AC +
Sbjct: 1   MVVLSQPALNQFF-------------LLKPFKSTPLFTGIPVVDLTHPDAKNLIVNACRD 60

Query: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120
            GFFK+VNHGVP+E ++ LE+E+  FF    SEK +A PP PFGYG+K+IG NGDVGWVE
Sbjct: 61  FGFFKLVNHGVPLELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVE 120

Query: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180
           YLLLNT+ +  S   L IF ++P   R+ V NYI+AV+NM   +LELMAEGL I+QRN  
Sbjct: 121 YLLLNTNPDVISPKSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTL 180

Query: 181 SKLVMDEESDSVFRVNHYPPCPELQALKERNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240
           S+L+ DE+SDS FR+NHYPPCPE+QAL  RN++GFGEHTDPQIISVLRSN+TSGLQI L 
Sbjct: 181 SRLLKDEKSDSCFRLNHYPPCPEVQAL-NRNLVGFGEHTDPQIISVLRSNSTSGLQICLT 240

Query: 241 DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI 300
           DG W+SVPPDQ SFFINVGD+LQVMTNGRFKSVKHRVL ++ KSR+SMIYFGGP LSE I
Sbjct: 241 DGTWVSVPPDQTSFFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENI 300

Query: 301 APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAA 346
           APLPS+M   E  LYKEFTW EYK++AY SRLADNRL  F++ AA
Sbjct: 301 APLPSVMLKGEECLYKEFTWCEYKKAAYTSRLADNRLAPFQKSAA 331

BLAST of ClCG07G010460 vs. ExPASy Swiss-Prot
Match: Q8LEA2 (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 386.7 bits (992), Expect = 2.7e-106
Identity = 188/308 (61.04%), Postives = 231/308 (75.00%), Query Frame = 0

Query: 37  FSEVPFIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQK 96
           FS +P ID+S P++K  +VKACE+ GFFKV+NHGV  E +S LE E+ +FFSLP SEK +
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 97  AAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISA 156
            A   PFGYGN +IGRNGDVGWVEYLL+N + +S S        + P   R+A+  Y ++
Sbjct: 75  VA-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTS 134

Query: 157 VRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCP--ELQALKERNMIG 216
           VR M  ++LE + +GL I+ RN  SKLV D+ +DS+ R+NHYPPCP    +    +N+IG
Sbjct: 135 VRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIG 194

Query: 217 FGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVK 276
           FGEHTDPQIISVLRSNNTSGLQI+L DG+WISVPPD  SFF NVGDSLQVMTNGRFKSV+
Sbjct: 195 FGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVR 254

Query: 277 HRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLAD 336
           HRVL N  KSR+SMIYF GP L+++IAPL  L+  E+  LY+EFTW EYK S YNSRL+D
Sbjct: 255 HRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSD 314

Query: 337 NRLVHFER 343
           NRL  FER
Sbjct: 315 NRLQQFER 321

BLAST of ClCG07G010460 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 6.9e-102
Identity = 178/303 (58.75%), Postives = 231/303 (76.24%), Query Frame = 0

Query: 40  VPFIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAP 99
           +P ++L+ P+AK  IVKACEE GFFKVVNHGV  E ++ LE E+  FF LP S K +A P
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 100 PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRN 159
           P P+GYGNK+IG NGDVGW+EYLLLN + + +S    ++F Q PQ  R +V  Y+  ++ 
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKE 150

Query: 160 MACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQALKERNMIGFGEHT 219
           ++ ++LE++AE L I+ R+  SK++ DE+SDS  R+NHYP   E    ++   +GFGEHT
Sbjct: 151 VSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEE--EAEKMVKVGFGEHT 210

Query: 220 DPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLT 279
           DPQIISVLRSNNT+GLQI + DG+W++VPPD +SFFINVGD+LQVMTNGRFKSVKHRVL 
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLA 270

Query: 280 NSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVH 339
           ++ +SRISMIYFGGPPLS+KIAPLP L+  ++  LYKEFTW +YK SAY S+L D RL  
Sbjct: 271 DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGL 330

Query: 340 FER 343
           FE+
Sbjct: 331 FEK 331

BLAST of ClCG07G010460 vs. ExPASy Swiss-Prot
Match: Q8S0S6 (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 2.5e-99
Identity = 181/306 (59.15%), Postives = 224/306 (73.20%), Query Frame = 0

Query: 37  FSEVPFIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQK 96
           FS VP +DL +P A + +V ACE  GFFKVVNHGV  + +   ESE+  FFS    +K +
Sbjct: 25  FSGVPVVDLGSPGAARAVVDACERYGFFKVVNHGVATDTMDKAESEAVRFFSQTQPDKDR 84

Query: 97  AAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISA 156
           + P  PFGYG+K+IG NGD+GW+EYLLL     S +D   +         R+A+N YIS 
Sbjct: 85  SGPAYPFGYGSKRIGFNGDMGWLEYLLLALDDASLAD---ACTVPSCAVFRAALNEYISG 144

Query: 157 VRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQALKERNMIGFG 216
           VR +A  ++E M+EGL I Q +  S LV  E SD VFRVNHYPPC  LQ L   ++ GFG
Sbjct: 145 VRKVAVRVMEAMSEGLGIAQADALSALVTAEGSDQVFRVNHYPPCRALQGL-GCSVTGFG 204

Query: 217 EHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHR 276
           EHTDPQ++SVLRSN TSGLQI+L DG W+SVP D++SFF+NVGDSLQV+TNGRFKSVKHR
Sbjct: 205 EHTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSDRDSFFVNVGDSLQVLTNGRFKSVKHR 264

Query: 277 VLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNR 336
           V+ NSLKSR+S IYFGGPPL+++IAPLP L+   E+SLYKEFTW EYK++AY SRL DNR
Sbjct: 265 VVANSLKSRVSFIYFGGPPLAQRIAPLPQLLGEGEQSLYKEFTWDEYKKAAYKSRLGDNR 324

Query: 337 LVHFER 343
           L  FE+
Sbjct: 325 LAQFEK 326

BLAST of ClCG07G010460 vs. ExPASy TrEMBL
Match: A0A1S3BRQ7 (gibberellin 2-beta-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103492969 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.3e-183
Identity = 331/346 (95.66%), Postives = 336/346 (97.11%), Query Frame = 0

Query: 1   MVVLSKPSIQQLYSNSFMRNSMSSSMAAAASSSAAFFSEVPFIDLSAPDAKQLIVKACEE 60
           MVVLSK SIQQL SNSFMRN    SMA+AASSSAAFFSEVP IDLS+PDAKQLIVKACEE
Sbjct: 1   MVVLSKQSIQQLSSNSFMRN----SMASAASSSAAFFSEVPLIDLSSPDAKQLIVKACEE 60

Query: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120
           LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE
Sbjct: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120

Query: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180
           Y+LLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRN F
Sbjct: 121 YILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNAF 180

Query: 181 SKLVMDEESDSVFRVNHYPPCPELQALKERNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240
           SKLVMDEESDSVFRVNHYPPCP++QALK  NMIGFGEHTDPQIISVLRSNNTSGLQISLA
Sbjct: 181 SKLVMDEESDSVFRVNHYPPCPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240

Query: 241 DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI 300
           DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI
Sbjct: 241 DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI 300

Query: 301 APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 347
           APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 342

BLAST of ClCG07G010460 vs. ExPASy TrEMBL
Match: A0A0A0KXI6 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G075200 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.1e-182
Identity = 329/346 (95.09%), Postives = 335/346 (96.82%), Query Frame = 0

Query: 1   MVVLSKPSIQQLYSNSFMRNSMSSSMAAAASSSAAFFSEVPFIDLSAPDAKQLIVKACEE 60
           MV+LSK SIQQL SNSFMRN    SMA+AASSS AF SEVP IDLS+PDAKQLIVKACEE
Sbjct: 1   MVILSKQSIQQLSSNSFMRN----SMASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEE 60

Query: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120
           LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE
Sbjct: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120

Query: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180
           Y+LLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF
Sbjct: 121 YILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180

Query: 181 SKLVMDEESDSVFRVNHYPPCPELQALKERNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240
           SKLVMDEESDSVFRVNHYPPCP++QALK  NMIGFGEHTDPQIISVLRSNNTSGLQISLA
Sbjct: 181 SKLVMDEESDSVFRVNHYPPCPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240

Query: 241 DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI 300
           DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI
Sbjct: 241 DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI 300

Query: 301 APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 347
           APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 342

BLAST of ClCG07G010460 vs. ExPASy TrEMBL
Match: A0A5A7V705 (Gibberellin 2-beta-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G001240 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.4e-176
Identity = 312/321 (97.20%), Postives = 317/321 (98.75%), Query Frame = 0

Query: 26  MAAAASSSAAFFSEVPFIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 85
           MA+AASSSAAFFSEVP IDLS+PDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN
Sbjct: 1   MASAASSSAAFFSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60

Query: 86  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 145
           FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEY+LLNTHLESNSDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120

Query: 146 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQ 205
           LRSAVNNYISAVRNMACEILELMAEGLKIQQRN FSKLVMDEESDSVFRVNHYPPCP++Q
Sbjct: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNAFSKLVMDEESDSVFRVNHYPPCPQIQ 180

Query: 206 ALKERNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 265
           ALK  NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM
Sbjct: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240

Query: 266 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 325
           TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300

Query: 326 SAYNSRLADNRLVHFERIAAS 347
           SAYNSRLADNRLVHFERIAAS
Sbjct: 301 SAYNSRLADNRLVHFERIAAS 321

BLAST of ClCG07G010460 vs. ExPASy TrEMBL
Match: S0BDZ9 (GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox1 PE=2 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 2.8e-175
Identity = 311/321 (96.88%), Postives = 316/321 (98.44%), Query Frame = 0

Query: 26  MAAAASSSAAFFSEVPFIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 85
           MA+AASSS AF SEVP IDLS+PDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN
Sbjct: 1   MASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTN 60

Query: 86  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQK 145
           FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEY+LLNTHLESNSDGFLSIFGQDPQK
Sbjct: 61  FFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQK 120

Query: 146 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQ 205
           LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCP++Q
Sbjct: 121 LRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPQIQ 180

Query: 206 ALKERNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 265
           ALK  NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM
Sbjct: 181 ALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVM 240

Query: 266 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 325
           TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR
Sbjct: 241 TNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKR 300

Query: 326 SAYNSRLADNRLVHFERIAAS 347
           SAYNSRLADNRLVHFERIAAS
Sbjct: 301 SAYNSRLADNRLVHFERIAAS 321

BLAST of ClCG07G010460 vs. ExPASy TrEMBL
Match: A0A6J1J0N2 (gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480835 PE=3 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 5.4e-174
Identity = 318/346 (91.91%), Postives = 328/346 (94.80%), Query Frame = 0

Query: 1   MVVLSKPSIQQLYSNSFMRNSMSSSMAAAASSSAAFFSEVPFIDLSAPDAKQLIVKACEE 60
           MVVLSKPSI+Q+ ++S+MRN    SMAAA SSSAAFFS VP IDLSAPDAK LIVKACEE
Sbjct: 1   MVVLSKPSIKQM-ASSYMRN----SMAAAGSSSAAFFSGVPLIDLSAPDAKHLIVKACEE 60

Query: 61  LGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVE 120
           LGFFKVVNHGVPMEFISTLESEST FFSLPLSEKQ+A PPSPFGYGNKQIGRNGDVGWVE
Sbjct: 61  LGFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVE 120

Query: 121 YLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVF 180
           YLLLNTHLES SDGFLSIFGQDPQKLRSAVN+YISAVRNMACEILELMAEGLKIQQRN F
Sbjct: 121 YLLLNTHLESKSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAF 180

Query: 181 SKLVMDEESDSVFRVNHYPPCPELQALKERNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240
           SKL+MDEESDSVFRVNHYPPCPEL ALK  NMIGFGEHTDPQIISVLRSNNTSGLQISLA
Sbjct: 181 SKLLMDEESDSVFRVNHYPPCPELHALKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLA 240

Query: 241 DGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKI 300
           DGNWISVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSEKI
Sbjct: 241 DGNWISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKI 300

Query: 301 APLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 347
           APLPSLMKGEE SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 APLPSLMKGEE-SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 340

BLAST of ClCG07G010460 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 386.7 bits (992), Expect = 1.9e-107
Identity = 188/308 (61.04%), Postives = 231/308 (75.00%), Query Frame = 0

Query: 37  FSEVPFIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQK 96
           FS +P ID+S P++K  +VKACE+ GFFKV+NHGV  E +S LE E+ +FFSLP SEK +
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 97  AAPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISA 156
            A   PFGYGN +IGRNGDVGWVEYLL+N + +S S        + P   R+A+  Y ++
Sbjct: 75  VA-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTS 134

Query: 157 VRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCP--ELQALKERNMIG 216
           VR M  ++LE + +GL I+ RN  SKLV D+ +DS+ R+NHYPPCP    +    +N+IG
Sbjct: 135 VRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIG 194

Query: 217 FGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVK 276
           FGEHTDPQIISVLRSNNTSGLQI+L DG+WISVPPD  SFF NVGDSLQVMTNGRFKSV+
Sbjct: 195 FGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVR 254

Query: 277 HRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLAD 336
           HRVL N  KSR+SMIYF GP L+++IAPL  L+  E+  LY+EFTW EYK S YNSRL+D
Sbjct: 255 HRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSD 314

Query: 337 NRLVHFER 343
           NRL  FER
Sbjct: 315 NRLQQFER 321

BLAST of ClCG07G010460 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 372.1 bits (954), Expect = 4.9e-103
Identity = 178/303 (58.75%), Postives = 231/303 (76.24%), Query Frame = 0

Query: 40  VPFIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAP 99
           +P ++L+ P+AK  IVKACEE GFFKVVNHGV  E ++ LE E+  FF LP S K +A P
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 100 PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRN 159
           P P+GYGNK+IG NGDVGW+EYLLLN + + +S    ++F Q PQ  R +V  Y+  ++ 
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKE 150

Query: 160 MACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQALKERNMIGFGEHT 219
           ++ ++LE++AE L I+ R+  SK++ DE+SDS  R+NHYP   E    ++   +GFGEHT
Sbjct: 151 VSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEE--EAEKMVKVGFGEHT 210

Query: 220 DPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLT 279
           DPQIISVLRSNNT+GLQI + DG+W++VPPD +SFFINVGD+LQVMTNGRFKSVKHRVL 
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLA 270

Query: 280 NSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVH 339
           ++ +SRISMIYFGGPPLS+KIAPLP L+  ++  LYKEFTW +YK SAY S+L D RL  
Sbjct: 271 DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGL 330

Query: 340 FER 343
           FE+
Sbjct: 331 FEK 331

BLAST of ClCG07G010460 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 347.8 bits (891), Expect = 9.9e-96
Identity = 173/303 (57.10%), Postives = 217/303 (71.62%), Query Frame = 0

Query: 40  VPFIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAP 99
           +P IDL+  DAK  IVKACEE GFFKV+NHGV  + ++ LE E+ NFF+L  S K KA P
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 100 PSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRN 159
           P PFGYG K+IG NGD+GW+EY+LLN +L   S    +IF   P   R AV  YI  ++ 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 160 MACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQALKERNMIGFGEHT 219
           M+ + LE++ E LKI+ +   S+LV  +ESDS  R+NHYP   E    +E   IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEETPVKEE---IGFGEHT 206

Query: 220 DPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLT 279
           DPQ+IS+LRSN+T GLQI + DG W+ V PD +SFF+ VGD+LQVMTNGRFKSVKHRV+T
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 280 NSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVH 339
           N+ +SRISMIYF GPPLSEKIAPL  L+  ++  LY EFTW +YK SAY ++L D RL  
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 326

Query: 340 FER 343
           FE+
Sbjct: 327 FEK 326

BLAST of ClCG07G010460 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 295.0 bits (754), Expect = 7.6e-80
Identity = 154/307 (50.16%), Postives = 198/307 (64.50%), Query Frame = 0

Query: 41  PFIDLSAPDAKQL---IVKACEELGFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKA 100
           P ID S  D  +L   IVKACE  GFFKV+NHGV  E I   E E   FF+ P S+K +A
Sbjct: 25  PVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGEEFFNKPESDKLRA 84

Query: 101 APPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAV 160
            P SPFGYG K IG NGD+G +EYLLL+ +  + +D   +I   DP K  SA N+YI  V
Sbjct: 85  GPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPFKFSSATNDYIRTV 144

Query: 161 RNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCPELQALKERNMIGFGE 220
           R++ACEI++L  E L  Q+ +  S+L+ D  SDS+ R+NHYPP P   AL     IGFGE
Sbjct: 145 RDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPAP--YALSGVGQIGFGE 204

Query: 221 HTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRV 280
           H+DPQI++VLRSN+  GL+I   DG WI +P D   FF+ VGD LQ +TNGRF SV+HRV
Sbjct: 205 HSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGDCLQALTNGRFTSVRHRV 264

Query: 281 LTNSLKS-RISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNR 340
           L N+ K  R+S +YF  PPL  KI+PLP ++  E    Y  FTW +YK++ Y+ RL   R
Sbjct: 265 LANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSFTWGDYKKATYSLRLDVPR 324

Query: 341 LVHFERI 344
           L  F+ +
Sbjct: 325 LEFFKTL 329

BLAST of ClCG07G010460 vs. TAIR 10
Match: AT1G47990.1 (gibberellin 2-oxidase 4 )

HSP 1 Score: 282.0 bits (720), Expect = 6.7e-76
Identity = 163/328 (49.70%), Postives = 202/328 (61.59%), Query Frame = 0

Query: 26  MAAAASSSAAFFSEVPFIDLS--APDAKQLIVKACEELGFFKVVNHGVPMEFISTLESES 85
           M   +    A   ++P ID+S         IVKACE LGFFKV+NHGV    IS +E ES
Sbjct: 1   MVKGSQKIVAVDQDIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQES 60

Query: 86  TNFFSLPLSEKQKAAPPS-PFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQD 145
            NFF+ P  EK+   P + PF YG + IG NGD G VEYLL +T    N   F S     
Sbjct: 61  INFFAKPAHEKKSVRPVNQPFRYGFRDIGLNGDSGEVEYLLFHT----NDPAFRS----- 120

Query: 146 PQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPPCP 205
                SAVN YI AV+ +A EIL+L AEGL +   + FS+L+   +SDSV RVNHYPP  
Sbjct: 121 QLSFSSAVNCYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSD 180

Query: 206 ELQALKERNM---------IGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNS 265
             Q   E N+         +GFGEHTDPQI++VLRSN   GLQ+S +DG W+SV PD ++
Sbjct: 181 --QFFGEANLSDQSVSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSA 240

Query: 266 FFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSL-MKGEER 325
           F +NVGD LQVMTNGRF SV+HR LT   +SR+S  YF GPPL  KI PL ++ M   + 
Sbjct: 241 FCVNVGDLLQVMTNGRFISVRHRALTYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQP 300

Query: 326 SLYKEFTWFEYKRSAYNSRLADNRLVHF 341
            LY+ FTW EYK+ AY+ RL D+RL  F
Sbjct: 301 RLYQTFTWGEYKKRAYSLRLEDSRLDMF 316

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879745.13.3e-18697.69gibberellin 2-beta-dioxygenase 1-like [Benincasa hispida][more]
XP_008451787.12.6e-18395.66PREDICTED: gibberellin 2-beta-dioxygenase 1 [Cucumis melo][more]
KGN53539.12.2e-18295.09hypothetical protein Csa_014892 [Cucumis sativus][more]
KAA0063004.19.1e-17697.20gibberellin 2-beta-dioxygenase 1 [Cucumis melo var. makuwa] >TYK16342.1 gibberel... [more]
NP_001292640.15.9e-17596.88gibberellin 2-beta-dioxygenase 1 [Cucumis sativus] >CCD28485.1 GA 2-oxidase [Cuc... [more]
Match NameE-valueIdentityDescription
Q9SQ803.9e-12967.34Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1[more]
Q9XG831.1e-12062.61Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=... [more]
Q8LEA22.7e-10661.04Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 ... [more]
Q9XFR96.9e-10258.75Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ... [more]
Q8S0S62.5e-9959.15Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2... [more]
Match NameE-valueIdentityDescription
A0A1S3BRQ71.3e-18395.66gibberellin 2-beta-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103492969 PE=3 SV... [more]
A0A0A0KXI61.1e-18295.09Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G... [more]
A0A5A7V7054.4e-17697.20Gibberellin 2-beta-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
S0BDZ92.8e-17596.88GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox1 PE=2 SV=1[more]
A0A6J1J0N25.4e-17491.91gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11148083... [more]
Match NameE-valueIdentityDescription
AT1G78440.11.9e-10761.04Arabidopsis thaliana gibberellin 2-oxidase 1 [more]
AT1G30040.14.9e-10358.75gibberellin 2-oxidase [more]
AT2G34555.19.9e-9657.10gibberellin 2-oxidase 3 [more]
AT1G02400.17.6e-8050.16gibberellin 2-oxidase 6 [more]
AT1G47990.16.7e-7649.70gibberellin 2-oxidase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 218..239
score: 32.23
coord: 254..280
score: 33.0
coord: 54..71
score: 54.55
NoneNo IPR availablePANTHERPTHR47990:SF125GIBBERELLIN 2-OXIDASEcoord: 30..343
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 30..343
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 35..334
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 190..292
e-value: 2.5E-27
score: 95.3
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 4..334
e-value: 2.2E-83
score: 282.3
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 40..131
e-value: 5.1E-16
score: 59.5
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 188..294
score: 12.370198

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG07G010460.1ClCG07G010460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045487 gibberellin catabolic process
biological_process GO:0009416 response to light stimulus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0052634 C-19 gibberellin 2-beta-dioxygenase activity
molecular_function GO:0046872 metal ion binding