ClCG07G005470 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG07G005470
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionRING-type E3 ubiquitin transferase
LocationCG_Chr07: 7795653 .. 7799554 (-)
RNA-Seq ExpressionClCG07G005470
SyntenyClCG07G005470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGATGATTTCTTCTTGGAGACGCCGGAGAGCCACCCGGCGAATTGCCAAGGGTGGGGTCAATAATGACATGGTGGAGTTGACAATCCCGAGCCATTTCCGGTGCCCGATATCGCTCGACTTGATGAAAGATCCGGTGACTTTGTCGACCGGGATCACGTATGATCGAGGTAGCATTGAGACTTGGATTGAAGGAGGGAATTTCAGCTGTCCCTTCACCAACCAGCCATTGCAGAGCATTGAGTCCATTCCTAATCATAACATAAGGAAGATGATTCAAGATTGGTGTGTTGAGAATAGAGCTTATGGGATTGAGAGGATTCCGACGCCAAGGGTTCCGGCCAGTCCGGCACAGGTTCGGGAGATTCTTGAGAAGATGGCTGCCGCGGCACGGCGGGGGGATTGTGATGGATGCAAGTCCATGGTGGAGAAGATCAAGAAATTAGGAAAAGAAAGTGAAAGGAACAAGAAATGCATTATTGGCAACGGAACAGGTTTATAAACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCTCCAATACCATGTTAAAACAAACTAGACTTACTAAGCTCGAGACTTCAAGATTCATGATTTACATATATATTTTGAAATGCAGGTAATATATTGTCATCAACATTTGAAGTGTTTTCGAAACCCGAAAATTTGGAGAGAAGCATAGAAGTTTTAGAGGAAATTTTATCAGCCATGGCAACCATGTTCCCTCTACAAGAAGAAGCCATTAAACACTTGAAATCAGAAACTTCATTGCAATCATTGGTATGGTTTGTAAAAGGAGGAGATATATCAGGAAGAAGAAATTCAGTTTTGGTATTGAAAGAGATAATTTCATCATACCCAGAAAAAGTTAATGAATTAGGTGAAATCCAAGGAGCATTAGAGGGTCTTGTTAAGCTCATAAAAGACCCAATTTGCTCATCTTCAAAAAAGGCCTCACTTTTCATAACATATCATCTCATAGCCTCCTCAACTTCTTCTTCTTCTTCTTCAAATCAAGAGAGGTTCACAAAAGCACTTCTTGAAATGGGGTTGGTCTCTTTGCTACTCGAAACCCTTGTCGACGCCGAGAGAAGCGTATGCGAGAGGGCATTGGGAGCGTTCGATGGCATTTGCGAAACCAAACAAGGAAGAGAAGAGGCATATGGGCACGCCTTGACGATGCCTGTGATTGTCAAAAAGATACTTAGAGTATCGGATTTGGCTACGGAATTATCGGTCTCCATTGTTTGGAAGCTTGTAAAGCATGAGAATAGAGAAGATGAAGAAAAAGAAGGAGGAGTTAAAGTAGAAGCACTTCAAGTGGGAGCATTTCAAAAGCTTTTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTCTTGAATAATCTTCATAGGGATAGGTTAGAATGTATTGATTCTTTGGATTTTAAGGACCTCAAAAGAACATTTTGA

mRNA sequence

ATGATGATGATTTCTTCTTGGAGACGCCGGAGAGCCACCCGGCGAATTGCCAAGGGTGGGGTCAATAATGACATGGTGGAGTTGACAATCCCGAGCCATTTCCGGTGCCCGATATCGCTCGACTTGATGAAAGATCCGGTGACTTTGTCGACCGGGATCACGTATGATCGAGGTAGCATTGAGACTTGGATTGAAGGAGGGAATTTCAGCTGTCCCTTCACCAACCAGCCATTGCAGAGCATTGAGTCCATTCCTAATCATAACATAAGGAAGATGATTCAAGATTGGTGTGTTGAGAATAGAGCTTATGGGATTGAGAGGATTCCGACGCCAAGGGTTCCGGCCAGTCCGGCACAGGTTCGGGAGATTCTTGAGAAGATGGCTGCCGCGGCACGGCGGGGGGATTGTGATGGATGCAAGTCCATGGTGGAGAAGATCAAGAAATTAGGAAAAGAAAGTGAAAGGAACAAGAAATGCATTATTGGCAACGGAACAGGTAATATATTGTCATCAACATTTGAAGTGTTTTCGAAACCCGAAAATTTGGAGAGAAGCATAGAAGTTTTAGAGGAAATTTTATCAGCCATGGCAACCATGTTCCCTCTACAAGAAGAAGCCATTAAACACTTGAAATCAGAAACTTCATTGCAATCATTGGTATGGTTTGTAAAAGGAGGAGATATATCAGGAAGAAGAAATTCAGTTTTGGTATTGAAAGAGATAATTTCATCATACCCAGAAAAAGTTAATGAATTAGGTGAAATCCAAGGAGCATTAGAGGGTCTTGTTAAGCTCATAAAAGACCCAATTTGCTCATCTTCAAAAAAGGCCTCACTTTTCATAACATATCATCTCATAGCCTCCTCAACTTCTTCTTCTTCTTCTTCAAATCAAGAGAGGTTCACAAAAGCACTTCTTGAAATGGGGTTGGTCTCTTTGCTACTCGAAACCCTTGTCGACGCCGAGAGAAGCGTATGCGAGAGGGCATTGGGAGCGTTCGATGGCATTTGCGAAACCAAACAAGGAAGAGAAGAGGCATATGGGCACGCCTTGACGATGCCTGTGATTGTCAAAAAGATACTTAGAGTATCGGATTTGGCTACGGAATTATCGGTCTCCATTGTTTGGAAGCTTGTAAAGCATGAGAATAGAGAAGATGAAGAAAAAGAAGGAGGAGTTAAAGTAGAAGCACTTCAAGTGGGAGCATTTCAAAAGCTTTTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTCTTGAATAATCTTCATAGGGATAGGTTAGAATGTATTGATTCTTTGGATTTTAAGGACCTCAAAAGAACATTTTGA

Coding sequence (CDS)

ATGATGATGATTTCTTCTTGGAGACGCCGGAGAGCCACCCGGCGAATTGCCAAGGGTGGGGTCAATAATGACATGGTGGAGTTGACAATCCCGAGCCATTTCCGGTGCCCGATATCGCTCGACTTGATGAAAGATCCGGTGACTTTGTCGACCGGGATCACGTATGATCGAGGTAGCATTGAGACTTGGATTGAAGGAGGGAATTTCAGCTGTCCCTTCACCAACCAGCCATTGCAGAGCATTGAGTCCATTCCTAATCATAACATAAGGAAGATGATTCAAGATTGGTGTGTTGAGAATAGAGCTTATGGGATTGAGAGGATTCCGACGCCAAGGGTTCCGGCCAGTCCGGCACAGGTTCGGGAGATTCTTGAGAAGATGGCTGCCGCGGCACGGCGGGGGGATTGTGATGGATGCAAGTCCATGGTGGAGAAGATCAAGAAATTAGGAAAAGAAAGTGAAAGGAACAAGAAATGCATTATTGGCAACGGAACAGGTAATATATTGTCATCAACATTTGAAGTGTTTTCGAAACCCGAAAATTTGGAGAGAAGCATAGAAGTTTTAGAGGAAATTTTATCAGCCATGGCAACCATGTTCCCTCTACAAGAAGAAGCCATTAAACACTTGAAATCAGAAACTTCATTGCAATCATTGGTATGGTTTGTAAAAGGAGGAGATATATCAGGAAGAAGAAATTCAGTTTTGGTATTGAAAGAGATAATTTCATCATACCCAGAAAAAGTTAATGAATTAGGTGAAATCCAAGGAGCATTAGAGGGTCTTGTTAAGCTCATAAAAGACCCAATTTGCTCATCTTCAAAAAAGGCCTCACTTTTCATAACATATCATCTCATAGCCTCCTCAACTTCTTCTTCTTCTTCTTCAAATCAAGAGAGGTTCACAAAAGCACTTCTTGAAATGGGGTTGGTCTCTTTGCTACTCGAAACCCTTGTCGACGCCGAGAGAAGCGTATGCGAGAGGGCATTGGGAGCGTTCGATGGCATTTGCGAAACCAAACAAGGAAGAGAAGAGGCATATGGGCACGCCTTGACGATGCCTGTGATTGTCAAAAAGATACTTAGAGTATCGGATTTGGCTACGGAATTATCGGTCTCCATTGTTTGGAAGCTTGTAAAGCATGAGAATAGAGAAGATGAAGAAAAAGAAGGAGGAGTTAAAGTAGAAGCACTTCAAGTGGGAGCATTTCAAAAGCTTTTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTCTTGAATAATCTTCATAGGGATAGGTTAGAATGTATTGATTCTTTGGATTTTAAGGACCTCAAAAGAACATTTTGA

Protein sequence

MMMISSWRRRRATRRIAKGGVNNDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIETWIEGGNFSCPFTNQPLQSIESIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKPENLERSIEVLEEILSAMATMFPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGALEGLVKLIKDPICSSSKKASLFITYHLIASSTSSSSSSNQERFTKALLEMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKILRVSDLATELSVSIVWKLVKHENREDEEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLECIDSLDFKDLKRTF
Homology
BLAST of ClCG07G005470 vs. NCBI nr
Match: XP_038892848.1 (U-box domain-containing protein 21-like [Benincasa hispida])

HSP 1 Score: 809.3 bits (2089), Expect = 1.7e-230
Identity = 413/452 (91.37%), Postives = 429/452 (94.91%), Query Frame = 0

Query: 2   MMISSWRRRRATRRIAKGGVNNDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIE 61
           MMISSWRRRRA RR+A+ GV+ D VELTIPSHFRCPISL+LMKDPVTLSTGITYDR SIE
Sbjct: 1   MMISSWRRRRAARRMARVGVSGDTVELTIPSHFRCPISLELMKDPVTLSTGITYDRASIE 60

Query: 62  TWIEGGNFSCPFTNQPLQSIESIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR 121
           TW+EGGNF+CPFTNQPLQSI+SIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR
Sbjct: 61  TWMEGGNFTCPFTNQPLQSIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR 120

Query: 122 EILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKPEN 181
           EILEKMA A RRGDCDGCKS+VEKIKKLGKESERNKKCII NGTGN LSSTFE FS PE 
Sbjct: 121 EILEKMATATRRGDCDGCKSLVEKIKKLGKESERNKKCIINNGTGNTLSSTFEAFSNPEK 180

Query: 182 LERSIEVLEEILSAMATMFPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEI 241
            E+SIE+LEEILSAM  MFPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEI
Sbjct: 181 FEKSIEILEEILSAMTMMFPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEI 240

Query: 242 ISSYPEKVNELGEIQGALEGLVKLIKDPICSSSKKASLFITYHLIASSTSSSSSSNQERF 301
           ISSYPEKVNELGEIQGALEGL KLIKDPICSSSKKASLF+TYH+IA    S+SS NQERF
Sbjct: 241 ISSYPEKVNELGEIQGALEGLFKLIKDPICSSSKKASLFMTYHVIA----STSSPNQERF 300

Query: 302 TKALLEMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKIL 361
           TKALL+MGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAY HALTMPVIVKKIL
Sbjct: 301 TKALLQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKIL 360

Query: 362 RVSDLATELSVSIVWKLVKHENREDEEKE-GGVKVEALQVGAFQKLLLLLQVGCSEWTKE 421
           RVSDLATELSVSIVWKLVKHE+REDEE+E GGVKVEALQVGAFQKLLLLLQVGCSEWTKE
Sbjct: 361 RVSDLATELSVSIVWKLVKHESREDEEEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKE 420

Query: 422 KATELLKLLNNLHRDRLECIDSLDFKDLKRTF 453
           KATELLKLLNNLHRDRLECIDSLDFKDLKRTF
Sbjct: 421 KATELLKLLNNLHRDRLECIDSLDFKDLKRTF 448

BLAST of ClCG07G005470 vs. NCBI nr
Match: XP_004142305.1 (U-box domain-containing protein 21 [Cucumis sativus] >KGN49607.1 hypothetical protein Csa_018466 [Cucumis sativus])

HSP 1 Score: 792.0 bits (2044), Expect = 2.8e-225
Identity = 402/450 (89.33%), Postives = 428/450 (95.11%), Query Frame = 0

Query: 3   MISSWRRRRATRRIAKGGVNNDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIET 62
           MISSWRRRRATRR+A+GG+++  +ELTIP+HFRCPISLDLMKDPVTLSTGITYDR SIET
Sbjct: 1   MISSWRRRRATRRMARGGLSH-TIELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIET 60

Query: 63  WIEGGNFSCPFTNQPLQSIESIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRE 122
           WIEGGNF+CPFTNQPLQ+I+SIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRE
Sbjct: 61  WIEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRE 120

Query: 123 ILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKPENL 182
           ILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCII NGTGN LSS FE FS PE  
Sbjct: 121 ILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNTLSSIFEAFSNPETF 180

Query: 183 ERSIEVLEEILSAMATMFPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEII 242
           E+ IE+LEEILSAM   FPLQEE+IKHLKSETSLQSLVWF+KGGDISGRRNSVLVLKEII
Sbjct: 181 EKRIEILEEILSAMTVAFPLQEESIKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEII 240

Query: 243 SSYPEKVNELGEIQGALEGLVKLIKDPICSSSKKASLFITYHLIASSTSSSSSSNQERFT 302
           SSYPEKV+ELGEIQGALEGL+KLIKDPICSSS+KASLFITYH+IA   S+SSSSN+ERFT
Sbjct: 241 SSYPEKVDELGEIQGALEGLIKLIKDPICSSSRKASLFITYHVIA---STSSSSNRERFT 300

Query: 303 KALLEMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKILR 362
           K LL+MGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAY HALTMPVIVKKILR
Sbjct: 301 KELLQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILR 360

Query: 363 VSDLATELSVSIVWKLVKHENREDEEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKA 422
           VSDLATELSV IVWKLVKHE++E+E  +GG+KVEALQVGAFQKLLLLLQVGCSEWTKEKA
Sbjct: 361 VSDLATELSVCIVWKLVKHESKEEE--DGGIKVEALQVGAFQKLLLLLQVGCSEWTKEKA 420

Query: 423 TELLKLLNNLHRDRLECIDSLDFKDLKRTF 453
           TELLKLLNNLHRDRLECIDSLDFKDLKRTF
Sbjct: 421 TELLKLLNNLHRDRLECIDSLDFKDLKRTF 444

BLAST of ClCG07G005470 vs. NCBI nr
Match: XP_008465536.1 (PREDICTED: U-box domain-containing protein 21-like [Cucumis melo])

HSP 1 Score: 771.2 bits (1990), Expect = 5.1e-219
Identity = 394/450 (87.56%), Postives = 421/450 (93.56%), Query Frame = 0

Query: 3   MISSWRRRRATRRIAKGGVNNDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIET 62
           MISSWRRRRAT R+ +G +  D VELTIP+HFRCPISLDLMKDPVTLSTGITYDR SIET
Sbjct: 1   MISSWRRRRATPRMPRGSL-GDPVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIET 60

Query: 63  WIEGGNFSCPFTNQPLQSIESIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRE 122
           WIEGGNF+CPFTNQPLQ+I+SIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR+
Sbjct: 61  WIEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRD 120

Query: 123 ILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKPENL 182
           ILEKM AAARRGD +GCKSMVEKIK LGKESERNKKCII NGTGN  SS FE FS PE  
Sbjct: 121 ILEKMTAAARRGDFEGCKSMVEKIKNLGKESERNKKCIIANGTGNTFSSIFEAFSNPETF 180

Query: 183 ERSIEVLEEILSAMATMFPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEII 242
           E+ IE+LEEILSAM  +FPLQEE+IKHLKSETSLQSL WF+KGGDISGRRNSVLVLKEII
Sbjct: 181 EKRIEILEEILSAMTMVFPLQEESIKHLKSETSLQSLAWFLKGGDISGRRNSVLVLKEII 240

Query: 243 SSYPEKVNELGEIQGALEGLVKLIKDPICSSSKKASLFITYHLIASSTSSSSSSNQERFT 302
           SSYPEKV+ELG IQGALEGL+KLIKDPICSSSKKASLFITYH+IA   S+SSSSNQ RFT
Sbjct: 241 SSYPEKVDELGVIQGALEGLIKLIKDPICSSSKKASLFITYHVIA---STSSSSNQGRFT 300

Query: 303 KALLEMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKILR 362
           KALL+MGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAY HALTMPVIVKKILR
Sbjct: 301 KALLQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILR 360

Query: 363 VSDLATELSVSIVWKLVKHENREDEEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKA 422
           VSDLATELSVSIVWKLVK+E++E++E +GG++VEALQVGAFQKLLLLLQVGCSEWTKEKA
Sbjct: 361 VSDLATELSVSIVWKLVKNESKEEKE-DGGIEVEALQVGAFQKLLLLLQVGCSEWTKEKA 420

Query: 423 TELLKLLNNLHRDRLECIDSLDFKDLKRTF 453
           TELLKLLNNLHRDRLECIDSLDFKDLKRTF
Sbjct: 421 TELLKLLNNLHRDRLECIDSLDFKDLKRTF 445

BLAST of ClCG07G005470 vs. NCBI nr
Match: KAA0056826.1 (U-box domain-containing protein 21-like [Cucumis melo var. makuwa] >TYJ99948.1 U-box domain-containing protein 21-like [Cucumis melo var. makuwa])

HSP 1 Score: 750.4 bits (1936), Expect = 9.3e-213
Identity = 382/433 (88.22%), Postives = 406/433 (93.76%), Query Frame = 0

Query: 20  GVNNDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIETWIEGGNFSCPFTNQPLQ 79
           G   D VELTIP+HFRCPISLDLMKDPVTLSTGITYDR SIETWIEGGNF+CPFTNQPLQ
Sbjct: 4   GSLGDPVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFTCPFTNQPLQ 63

Query: 80  SIESIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKMAAAARRGDCDGC 139
           +I+SIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR+ILEKM AAARRGD +GC
Sbjct: 64  TIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRDILEKMTAAARRGDFEGC 123

Query: 140 KSMVEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKPENLERSIEVLEEILSAMATM 199
           KSMVEKIK LGKESERNKKCII NGTGN  SS FE FS PE  E+ IE+LEEILSAM  +
Sbjct: 124 KSMVEKIKNLGKESERNKKCIIANGTGNTFSSIFEAFSNPETFEKRIEILEEILSAMTMV 183

Query: 200 FPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGAL 259
           FPLQEE+IKHLKSETSLQSL WF+KGGDISGRRNSVLVLKEIISSYPEKV+ELG IQGAL
Sbjct: 184 FPLQEESIKHLKSETSLQSLAWFLKGGDISGRRNSVLVLKEIISSYPEKVDELGVIQGAL 243

Query: 260 EGLVKLIKDPICSSSKKASLFITYHLIASSTSSSSSSNQERFTKALLEMGLVSLLLETLV 319
           EGL+KLIKDPICSSSKKASLFITYH+IA   S+SSSSNQ RFTKALL+MGLVSLLLETLV
Sbjct: 244 EGLIKLIKDPICSSSKKASLFITYHVIA---STSSSSNQGRFTKALLQMGLVSLLLETLV 303

Query: 320 DAERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKILRVSDLATELSVSIVWKLV 379
           DAERSVCERALGAFDGICETKQGREEAY HALTMPVIVKKILRVSDLATELSVSIVWKLV
Sbjct: 304 DAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLV 363

Query: 380 KHENREDEEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLEC 439
           K+E++E++E +GG++VEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLEC
Sbjct: 364 KNESKEEKE-DGGIEVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLEC 423

Query: 440 IDSLDFKDLKRTF 453
           IDSLDFKDLKRTF
Sbjct: 424 IDSLDFKDLKRTF 432

BLAST of ClCG07G005470 vs. NCBI nr
Match: XP_022996865.1 (U-box domain-containing protein 21-like [Cucurbita maxima])

HSP 1 Score: 746.9 bits (1927), Expect = 1.0e-211
Identity = 379/452 (83.85%), Postives = 411/452 (90.93%), Query Frame = 0

Query: 2   MMISSWRRRRATRRIAKGGVNNDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIE 61
           MM+ SWRRRRATRR+A+GG    M ELTIPSHF CPISLDLMKDPVT+STGITYDR +IE
Sbjct: 1   MMMFSWRRRRATRRMARGGGGGSMTELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIE 60

Query: 62  TWIEGGNFSCPFTNQPLQSIESIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR 121
           TWIEGGNFSCPFTNQPL +I+SIPNHNIRKMIQ WCVENRA+GIERIPTPRVPASP QVR
Sbjct: 61  TWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVR 120

Query: 122 EILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKPEN 181
           EIL+K+AAAA R DC+ CKSMVEKIK L +ESERNKKCII NGTGN+LSS FE FSKPE 
Sbjct: 121 EILDKIAAAAGREDCEECKSMVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPER 180

Query: 182 LERSIEVLEEILSAMATMFPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEI 241
            E+ +E+LEEILS MA +FPLQEEAIKHLKSE S+Q+LVWFV+G D S RR+S+LVLKEI
Sbjct: 181 FEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEI 240

Query: 242 ISSYPEKVNELGEIQGALEGLVKLIKDPICSSSKKASLFITYHLIASSTSSSSSSNQERF 301
           ISSYPEKV+ELGEIQG LEG++KLIK+PICSSSKKASLFITYH IA      SSSNQE F
Sbjct: 241 ISSYPEKVSELGEIQGVLEGVMKLIKEPICSSSKKASLFITYHAIA------SSSNQEGF 300

Query: 302 TKALLEMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKIL 361
           TKA L+MGLVSLLLETL+DAERSVCERALG FDGICETKQGREEAY HALTMPVIVKKIL
Sbjct: 301 TKAFLQMGLVSLLLETLIDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKIL 360

Query: 362 RVSDLATELSVSIVWKLVKHENREDE-EKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKE 421
           RVSDLATELSVSIVWKLVKHE+REDE E++GGVKVEALQVGAFQKLLLLLQVGCSEWTKE
Sbjct: 361 RVSDLATELSVSIVWKLVKHESREDEDEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKE 420

Query: 422 KATELLKLLNNLHRDRLECIDSLDFKDLKRTF 453
           KATELLKLLNNLHR+RLECIDSLDFKDLKRTF
Sbjct: 421 KATELLKLLNNLHRERLECIDSLDFKDLKRTF 446

BLAST of ClCG07G005470 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 2.1e-103
Identity = 210/427 (49.18%), Postives = 278/427 (65.11%), Query Frame = 0

Query: 27  ELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIETWIEGGNFSCPFTNQPLQSIESIPN 86
           E+TIP  F+CPIS+DLMKDPV +STGITYDR SIETWI  GN +CP TN  L + + IPN
Sbjct: 28  EITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPN 87

Query: 87  HNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKMAAAARRGDCDGCKSMVEKI 146
           H IRKMIQ WCVE  +  I+RIPTPRVP  P +V EI  K+++A RRGD + C  ++EKI
Sbjct: 88  HTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKI 147

Query: 147 KKLGKESERNKKCIIGNGTGNILSSTFEVFSKPENLERSIEVLEEILSAMATMFPLQEEA 206
           KKLG ESE+N+KC+  N  G +L   F+ FS  E L     +L EILS +  MFP+  E 
Sbjct: 148 KKLGDESEKNRKCVNENSVGWVLCDCFDKFSGDEKL---TFMLNEILSLLTWMFPIGLEG 207

Query: 207 IKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGALEGLVKLI 266
           I  L S TS + +   +K  D S R+N+  ++KEI+S    +V+      G  E LVKLI
Sbjct: 208 ISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALVKLI 267

Query: 267 KDPICSSSKKASLFITYHLIASSTSSSSSSNQERFTKALLEMGLVSLLLETLVDAERSVC 326
           +D + SSS K+SL   Y ++      +S           LE+GLVS+ +E +VDAE SVC
Sbjct: 268 RDSVSSSSTKSSLIAIYQMVLQKPEIASE---------FLEIGLVSITVEMIVDAENSVC 327

Query: 327 ERALGAFDGICETKQGREEAYGHALTMPVIVKKILRVSDLATELSVSIVWKLVKHENRED 386
           E+AL   D ICET+ GREE   +AL MP++VKKI +VS+LAT  S+S++ KL K  N   
Sbjct: 328 EKALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVA 387

Query: 387 EEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLECIDSL-DF 446
            E       +A+++GAFQK+LL+LQVG  E TKEKATELLK++N   +   +C+DSL +F
Sbjct: 388 VE-------DAVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEF 435

Query: 447 KDLKRTF 453
           K +K+ F
Sbjct: 448 KYIKKPF 435

BLAST of ClCG07G005470 vs. ExPASy Swiss-Prot
Match: Q9C8D1 (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 3.0e-89
Identity = 192/436 (44.04%), Postives = 276/436 (63.30%), Query Frame = 0

Query: 23  NDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIETWIEGGNFSCPFTNQPLQSIE 82
           ++ VE+TIPS F+CPIS +LMKDPV +++GITYDR +IE W E G  +CP TN  L S+E
Sbjct: 26  SEEVEITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLE 85

Query: 83  SIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKMAAAARRGDCDGCKSM 142
            IPNH IR+MIQ WC  +   GIERIPTPRVP +  QV EI E+++AA RRGD   C  M
Sbjct: 86  QIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEM 145

Query: 143 VEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKPENLERSIEVLEEILSAMATMFPL 202
           V K+ +LGKESERN+KC+  NG G +L   F+ FS  EN   S+ +LEE +S +  M P+
Sbjct: 146 VTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFS--ENANASL-LLEETVSVLTWMLPI 205

Query: 203 QEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGALEGL 262
             E    L + +S   LV  ++ GD    +N+  ++KE++      V+ L +I G  E  
Sbjct: 206 GLEGQSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAF 265

Query: 263 VKLI-KDPICSSSKKASLFITYHLIASSTSSSSSSNQERFTKALLEMGLVSLLLETLVDA 322
           +K I +D  C +    SL   +H+I         +NQE  ++  LE+ LV++ +E LVD+
Sbjct: 266 MKSINRDSTCVN----SLISIHHMIL--------TNQETVSR-FLELDLVNITVEMLVDS 325

Query: 323 ERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKILRVSDLATELSVSIVWKLVKH 382
           E SVCE+AL   + ICETK+GRE+   + L +P++VKKIL++S+   +  VS++WK+ K 
Sbjct: 326 ENSVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCKS 385

Query: 383 ENREDEEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHR-----DR 442
            +  + E       EAL++GAF+KL+++LQVGC E TKEK TELLK++N + +     DR
Sbjct: 386 GDGSEVE-------EALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDR 431

Query: 443 LECIDSLDFKDLKRTF 453
                S++FK +K+ F
Sbjct: 446 -SYSSSIEFKHVKKPF 431

BLAST of ClCG07G005470 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.8e-52
Identity = 150/428 (35.05%), Postives = 228/428 (53.27%), Query Frame = 0

Query: 28  LTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIETWIE-GGNFSCPFTNQPLQSIESIPN 87
           + IP HFRCPISL+LM+DPVT+ TG TYDR SIE+W+  G N +CP T  PL     IPN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 88  HNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKMAAAARRGDCDGCKSMVEKI 147
           H +R++IQ+WCV NR+ G+ERIPTP+ PA P  VR +L +  A+A  G     +S    +
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQ--ASAITGTHVSVRSRAAAL 131

Query: 148 KKL---GKESERNKKCIIGNGTGNILSSTFEVFSKPENLERSIEVLEEILSAMATMFPLQ 207
           ++L    ++S++N+  I  +    IL     +FS+      S E++ E L A+  M P+ 
Sbjct: 132 RRLRGFARDSDKNRVLIAAHNATEILIKI--LFSE----TTSSELVSESL-ALLVMLPIT 191

Query: 208 E--EAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEIISSYPEKVNELGEI---QGA 267
           E  + +        ++ L   +    I  R N+  ++ EI+S+  +  +  G I   +  
Sbjct: 192 EPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALI-EIVSTGTKSADLKGSISNSESV 251

Query: 268 LEGLVKLIKDPICSSSKKASLFITYHLIASSTSSSSSSNQERFTKALLEMGLVSLLLETL 327
            EG++ L+++PI   S + +L I    + +  S  S+ +        +  G   +L++ L
Sbjct: 252 FEGVLDLLRNPI---SSRRALKIGIKTLFALCSVKSTRH------IAITAGAPEILIDRL 311

Query: 328 -VDAERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKILRVSDLATELSVSIVWK 387
             D +R   ERAL   + +C T +G      HALT+P++VK ILRVSD ATE +   +  
Sbjct: 312 AADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLA 371

Query: 388 LVKHENREDEEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRL 446
           L   E R  EE  G         G   +LLL++Q  C+E  K+KA +LLKLL +   D  
Sbjct: 372 LCTAEERWREEAAG--------AGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYN 412

BLAST of ClCG07G005470 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 2.1e-50
Identity = 136/408 (33.33%), Postives = 208/408 (50.98%), Query Frame = 0

Query: 28  LTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIETWIEGGNFSCPFTNQPLQSIESIPNH 87
           + IP HFRCPISLDLM DPVT+STG TYDR SI++WI  GN +CP T   L     IPNH
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNH 71

Query: 88  NIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKMAA-AARRGDCDGCKSMVEKI 147
            +R++IQ+WCV NR+ G+ERIPTP+ PA P  VR +L + +A            + + ++
Sbjct: 72  TLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRL 131

Query: 148 KKLGKESERNKKCIIGNGTGNILSSTFEVFSKPENLERSIEVLEEILSAMATMFPLQEEA 207
           + L ++SE+N+  I G+    IL     +F+  E    S E++ E L+ +  +   + E 
Sbjct: 132 RGLARDSEKNRVLIAGHNAREILVRI--LFADIETTSLSSELVSESLALLVLLHMTETEC 191

Query: 208 IKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEIISSYPEKVNEL--GEIQGALEGLVK 267
                  + +  +   +    I  R N+  +++ +++       +L         EG++ 
Sbjct: 192 EAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEGVLD 251

Query: 268 LIKDPICSSSKKASLFITYHLIASSTSSSSSSNQERFTKALLEMGLVSLLLETL-VDAER 327
           L+K+PI   S + +L I    I +      + +        +  G   +L++ L  D +R
Sbjct: 252 LLKNPI---SSRRALKIGIKAIFALCLVKQTRH------LAISAGAPGILIDRLAADFDR 311

Query: 328 SVCERALGAFDGICETKQGREEAYGHALTMPVIVKKILRVSDLATELSVSIVWKLVKHEN 387
              ER L   + +C   +G      HALT+P++VK ILRVSD ATE +   +  L   E 
Sbjct: 312 CDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTAEE 371

Query: 388 REDEEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNN 432
           R         + EA   G   +LLLL+Q  C+E  K KA  LLKLL +
Sbjct: 372 R--------CRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRD 400

BLAST of ClCG07G005470 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.0e-48
Identity = 130/422 (30.81%), Postives = 221/422 (52.37%), Query Frame = 0

Query: 19  GGVNNDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIETWI-EGGNFSCPFTNQP 78
           GG+ ++  E+ IP  F CPISL++MKDPV +STGITYDR SIE W+  G   SCP T Q 
Sbjct: 3   GGIMDE--EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQD 62

Query: 79  LQSIESIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKMAAAARRGDCD 138
           +   +  PNH +R++IQ WC  N +YG+ERIPTPR P   +++ +++   A++      +
Sbjct: 63  ITDADLTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSH-----E 122

Query: 139 GCKSMVEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKPENLERSIEVLEEILSAMA 198
                +++++++  E+  NK+C+   G    L++   V +  EN   + E L  +L  + 
Sbjct: 123 NQVKCLKRLRQIVSENATNKRCLEAAGVPEFLANI--VSNDSENGSLTDEAL-NLLYHLE 182

Query: 199 TMFPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQG 258
           T   + +  + + K    ++SL   ++ G    R  + L+LK I+    + +  +     
Sbjct: 183 TSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNIL-EVADPMQSMTLKPE 242

Query: 259 ALEGLVKLIKDPICSSSKKASLFITYHLIASSTSSSSSSNQERFTKALLEMGLVSLLLET 318
               +V+++ D I   + KA++ I  ++     +   +          +E G++S+++E 
Sbjct: 243 VFTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKA----------VEAGVISVIIEL 302

Query: 319 LVD----AERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKILRVSDLATELSVS 378
           L+D    +ER   E A+   D +C+  +GR E   H   + V+ KKILRVS  A++ +V 
Sbjct: 303 LMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVR 362

Query: 379 IVWKLVKHENREDEEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLH 436
           ++  + +            +  E LQ+G   KL L+LQV C   TKEKA ELLKL   + 
Sbjct: 363 VLLSVGRF------CATPALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVW 397

BLAST of ClCG07G005470 vs. ExPASy TrEMBL
Match: A0A0A0KMI8 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G023870 PE=4 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 1.3e-225
Identity = 402/450 (89.33%), Postives = 428/450 (95.11%), Query Frame = 0

Query: 3   MISSWRRRRATRRIAKGGVNNDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIET 62
           MISSWRRRRATRR+A+GG+++  +ELTIP+HFRCPISLDLMKDPVTLSTGITYDR SIET
Sbjct: 1   MISSWRRRRATRRMARGGLSH-TIELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIET 60

Query: 63  WIEGGNFSCPFTNQPLQSIESIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRE 122
           WIEGGNF+CPFTNQPLQ+I+SIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRE
Sbjct: 61  WIEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRE 120

Query: 123 ILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKPENL 182
           ILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCII NGTGN LSS FE FS PE  
Sbjct: 121 ILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNTLSSIFEAFSNPETF 180

Query: 183 ERSIEVLEEILSAMATMFPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEII 242
           E+ IE+LEEILSAM   FPLQEE+IKHLKSETSLQSLVWF+KGGDISGRRNSVLVLKEII
Sbjct: 181 EKRIEILEEILSAMTVAFPLQEESIKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEII 240

Query: 243 SSYPEKVNELGEIQGALEGLVKLIKDPICSSSKKASLFITYHLIASSTSSSSSSNQERFT 302
           SSYPEKV+ELGEIQGALEGL+KLIKDPICSSS+KASLFITYH+IA   S+SSSSN+ERFT
Sbjct: 241 SSYPEKVDELGEIQGALEGLIKLIKDPICSSSRKASLFITYHVIA---STSSSSNRERFT 300

Query: 303 KALLEMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKILR 362
           K LL+MGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAY HALTMPVIVKKILR
Sbjct: 301 KELLQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILR 360

Query: 363 VSDLATELSVSIVWKLVKHENREDEEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKA 422
           VSDLATELSV IVWKLVKHE++E+E  +GG+KVEALQVGAFQKLLLLLQVGCSEWTKEKA
Sbjct: 361 VSDLATELSVCIVWKLVKHESKEEE--DGGIKVEALQVGAFQKLLLLLQVGCSEWTKEKA 420

Query: 423 TELLKLLNNLHRDRLECIDSLDFKDLKRTF 453
           TELLKLLNNLHRDRLECIDSLDFKDLKRTF
Sbjct: 421 TELLKLLNNLHRDRLECIDSLDFKDLKRTF 444

BLAST of ClCG07G005470 vs. ExPASy TrEMBL
Match: A0A1S3CPH4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103503164 PE=4 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.5e-219
Identity = 394/450 (87.56%), Postives = 421/450 (93.56%), Query Frame = 0

Query: 3   MISSWRRRRATRRIAKGGVNNDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIET 62
           MISSWRRRRAT R+ +G +  D VELTIP+HFRCPISLDLMKDPVTLSTGITYDR SIET
Sbjct: 1   MISSWRRRRATPRMPRGSL-GDPVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIET 60

Query: 63  WIEGGNFSCPFTNQPLQSIESIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRE 122
           WIEGGNF+CPFTNQPLQ+I+SIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR+
Sbjct: 61  WIEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRD 120

Query: 123 ILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKPENL 182
           ILEKM AAARRGD +GCKSMVEKIK LGKESERNKKCII NGTGN  SS FE FS PE  
Sbjct: 121 ILEKMTAAARRGDFEGCKSMVEKIKNLGKESERNKKCIIANGTGNTFSSIFEAFSNPETF 180

Query: 183 ERSIEVLEEILSAMATMFPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEII 242
           E+ IE+LEEILSAM  +FPLQEE+IKHLKSETSLQSL WF+KGGDISGRRNSVLVLKEII
Sbjct: 181 EKRIEILEEILSAMTMVFPLQEESIKHLKSETSLQSLAWFLKGGDISGRRNSVLVLKEII 240

Query: 243 SSYPEKVNELGEIQGALEGLVKLIKDPICSSSKKASLFITYHLIASSTSSSSSSNQERFT 302
           SSYPEKV+ELG IQGALEGL+KLIKDPICSSSKKASLFITYH+IA   S+SSSSNQ RFT
Sbjct: 241 SSYPEKVDELGVIQGALEGLIKLIKDPICSSSKKASLFITYHVIA---STSSSSNQGRFT 300

Query: 303 KALLEMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKILR 362
           KALL+MGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAY HALTMPVIVKKILR
Sbjct: 301 KALLQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILR 360

Query: 363 VSDLATELSVSIVWKLVKHENREDEEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKA 422
           VSDLATELSVSIVWKLVK+E++E++E +GG++VEALQVGAFQKLLLLLQVGCSEWTKEKA
Sbjct: 361 VSDLATELSVSIVWKLVKNESKEEKE-DGGIEVEALQVGAFQKLLLLLQVGCSEWTKEKA 420

Query: 423 TELLKLLNNLHRDRLECIDSLDFKDLKRTF 453
           TELLKLLNNLHRDRLECIDSLDFKDLKRTF
Sbjct: 421 TELLKLLNNLHRDRLECIDSLDFKDLKRTF 445

BLAST of ClCG07G005470 vs. ExPASy TrEMBL
Match: A0A5D3BPA4 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold701G00820 PE=4 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 4.5e-213
Identity = 382/433 (88.22%), Postives = 406/433 (93.76%), Query Frame = 0

Query: 20  GVNNDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIETWIEGGNFSCPFTNQPLQ 79
           G   D VELTIP+HFRCPISLDLMKDPVTLSTGITYDR SIETWIEGGNF+CPFTNQPLQ
Sbjct: 4   GSLGDPVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFTCPFTNQPLQ 63

Query: 80  SIESIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKMAAAARRGDCDGC 139
           +I+SIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR+ILEKM AAARRGD +GC
Sbjct: 64  TIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRDILEKMTAAARRGDFEGC 123

Query: 140 KSMVEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKPENLERSIEVLEEILSAMATM 199
           KSMVEKIK LGKESERNKKCII NGTGN  SS FE FS PE  E+ IE+LEEILSAM  +
Sbjct: 124 KSMVEKIKNLGKESERNKKCIIANGTGNTFSSIFEAFSNPETFEKRIEILEEILSAMTMV 183

Query: 200 FPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGAL 259
           FPLQEE+IKHLKSETSLQSL WF+KGGDISGRRNSVLVLKEIISSYPEKV+ELG IQGAL
Sbjct: 184 FPLQEESIKHLKSETSLQSLAWFLKGGDISGRRNSVLVLKEIISSYPEKVDELGVIQGAL 243

Query: 260 EGLVKLIKDPICSSSKKASLFITYHLIASSTSSSSSSNQERFTKALLEMGLVSLLLETLV 319
           EGL+KLIKDPICSSSKKASLFITYH+IA   S+SSSSNQ RFTKALL+MGLVSLLLETLV
Sbjct: 244 EGLIKLIKDPICSSSKKASLFITYHVIA---STSSSSNQGRFTKALLQMGLVSLLLETLV 303

Query: 320 DAERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKILRVSDLATELSVSIVWKLV 379
           DAERSVCERALGAFDGICETKQGREEAY HALTMPVIVKKILRVSDLATELSVSIVWKLV
Sbjct: 304 DAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLV 363

Query: 380 KHENREDEEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLEC 439
           K+E++E++E +GG++VEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLEC
Sbjct: 364 KNESKEEKE-DGGIEVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLEC 423

Query: 440 IDSLDFKDLKRTF 453
           IDSLDFKDLKRTF
Sbjct: 424 IDSLDFKDLKRTF 432

BLAST of ClCG07G005470 vs. ExPASy TrEMBL
Match: A0A6J1K7Y6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491979 PE=4 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 5.0e-212
Identity = 379/452 (83.85%), Postives = 411/452 (90.93%), Query Frame = 0

Query: 2   MMISSWRRRRATRRIAKGGVNNDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIE 61
           MM+ SWRRRRATRR+A+GG    M ELTIPSHF CPISLDLMKDPVT+STGITYDR +IE
Sbjct: 1   MMMFSWRRRRATRRMARGGGGGSMTELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIE 60

Query: 62  TWIEGGNFSCPFTNQPLQSIESIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVR 121
           TWIEGGNFSCPFTNQPL +I+SIPNHNIRKMIQ WCVENRA+GIERIPTPRVPASP QVR
Sbjct: 61  TWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVR 120

Query: 122 EILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKPEN 181
           EIL+K+AAAA R DC+ CKSMVEKIK L +ESERNKKCII NGTGN+LSS FE FSKPE 
Sbjct: 121 EILDKIAAAAGREDCEECKSMVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPER 180

Query: 182 LERSIEVLEEILSAMATMFPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEI 241
            E+ +E+LEEILS MA +FPLQEEAIKHLKSE S+Q+LVWFV+G D S RR+S+LVLKEI
Sbjct: 181 FEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEI 240

Query: 242 ISSYPEKVNELGEIQGALEGLVKLIKDPICSSSKKASLFITYHLIASSTSSSSSSNQERF 301
           ISSYPEKV+ELGEIQG LEG++KLIK+PICSSSKKASLFITYH IA      SSSNQE F
Sbjct: 241 ISSYPEKVSELGEIQGVLEGVMKLIKEPICSSSKKASLFITYHAIA------SSSNQEGF 300

Query: 302 TKALLEMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKIL 361
           TKA L+MGLVSLLLETL+DAERSVCERALG FDGICETKQGREEAY HALTMPVIVKKIL
Sbjct: 301 TKAFLQMGLVSLLLETLIDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKIL 360

Query: 362 RVSDLATELSVSIVWKLVKHENREDE-EKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKE 421
           RVSDLATELSVSIVWKLVKHE+REDE E++GGVKVEALQVGAFQKLLLLLQVGCSEWTKE
Sbjct: 361 RVSDLATELSVSIVWKLVKHESREDEDEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKE 420

Query: 422 KATELLKLLNNLHRDRLECIDSLDFKDLKRTF 453
           KATELLKLLNNLHR+RLECIDSLDFKDLKRTF
Sbjct: 421 KATELLKLLNNLHRERLECIDSLDFKDLKRTF 446

BLAST of ClCG07G005470 vs. ExPASy TrEMBL
Match: A0A6J1FPJ6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447642 PE=4 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 1.6e-210
Identity = 379/454 (83.48%), Postives = 409/454 (90.09%), Query Frame = 0

Query: 2   MMISSWRRRRATRRIAK--GGVNNDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGS 61
           MM+ SWRRRRATRR+A+  GG    M ELTIPSHF CPISLDLMKDPVT+STGITYDR +
Sbjct: 1   MMMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRAN 60

Query: 62  IETWIEGGNFSCPFTNQPLQSIESIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQ 121
           IETWIEGGNFSCPFTNQPL +I+SIPNHNIRKMIQ WCVENRA+GIERIPTPRVPASP Q
Sbjct: 61  IETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQ 120

Query: 122 VREILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKP 181
           VREIL+K+AAA RR DC+GCKS+VEKIK L +ESERNKKCII NGTGN+LSS FE FSKP
Sbjct: 121 VREILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKP 180

Query: 182 ENLERSIEVLEEILSAMATMFPLQEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLK 241
           E  E+ +E+LEEILS MA +FPLQEEAIKHLKSE S+Q+LVWFV+G D S RR+S+LVLK
Sbjct: 181 ERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLK 240

Query: 242 EIISSYPEKVNELGEIQGALEGLVKLIKDPICSSSKKASLFITYHLIASSTSSSSSSNQE 301
           EIISSYPEKV+ELGEIQG LEGL+KLIK+PICSSSKKASLFITYH IA      SSSNQE
Sbjct: 241 EIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIA------SSSNQE 300

Query: 302 RFTKALLEMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKK 361
            FTKA L+MGLV LLLETLVDAERSVCERALG FDGICETKQGREEAY HALTMPVIVKK
Sbjct: 301 GFTKAFLQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKK 360

Query: 362 ILRVSDLATELSVSIVWKLVKHENREDEEKE-GGVKVEALQVGAFQKLLLLLQVGCSEWT 421
           ILRVSDL TELSVSIVWKLVKHE+RE+EE E GGVKVEALQVGAFQKLLLLLQVGCSEWT
Sbjct: 361 ILRVSDLVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWT 420

Query: 422 KEKATELLKLLNNLHRDRLECIDSLDFKDLKRTF 453
           KEKATELLKLLNNLHR+RLECIDSLDFKDLKRTF
Sbjct: 421 KEKATELLKLLNNLHRERLECIDSLDFKDLKRTF 448

BLAST of ClCG07G005470 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.5e-104
Identity = 210/427 (49.18%), Postives = 278/427 (65.11%), Query Frame = 0

Query: 27  ELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIETWIEGGNFSCPFTNQPLQSIESIPN 86
           E+TIP  F+CPIS+DLMKDPV +STGITYDR SIETWI  GN +CP TN  L + + IPN
Sbjct: 28  EITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPN 87

Query: 87  HNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKMAAAARRGDCDGCKSMVEKI 146
           H IRKMIQ WCVE  +  I+RIPTPRVP  P +V EI  K+++A RRGD + C  ++EKI
Sbjct: 88  HTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEKI 147

Query: 147 KKLGKESERNKKCIIGNGTGNILSSTFEVFSKPENLERSIEVLEEILSAMATMFPLQEEA 206
           KKLG ESE+N+KC+  N  G +L   F+ FS  E L     +L EILS +  MFP+  E 
Sbjct: 148 KKLGDESEKNRKCVNENSVGWVLCDCFDKFSGDEKL---TFMLNEILSLLTWMFPIGLEG 207

Query: 207 IKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGALEGLVKLI 266
           I  L S TS + +   +K  D S R+N+  ++KEI+S    +V+      G  E LVKLI
Sbjct: 208 ISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALVKLI 267

Query: 267 KDPICSSSKKASLFITYHLIASSTSSSSSSNQERFTKALLEMGLVSLLLETLVDAERSVC 326
           +D + SSS K+SL   Y ++      +S           LE+GLVS+ +E +VDAE SVC
Sbjct: 268 RDSVSSSSTKSSLIAIYQMVLQKPEIASE---------FLEIGLVSITVEMIVDAENSVC 327

Query: 327 ERALGAFDGICETKQGREEAYGHALTMPVIVKKILRVSDLATELSVSIVWKLVKHENRED 386
           E+AL   D ICET+ GREE   +AL MP++VKKI +VS+LAT  S+S++ KL K  N   
Sbjct: 328 EKALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVA 387

Query: 387 EEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLECIDSL-DF 446
            E       +A+++GAFQK+LL+LQVG  E TKEKATELLK++N   +   +C+DSL +F
Sbjct: 388 VE-------DAVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEF 435

Query: 447 KDLKRTF 453
           K +K+ F
Sbjct: 448 KYIKKPF 435

BLAST of ClCG07G005470 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 330.5 bits (846), Expect = 2.1e-90
Identity = 192/436 (44.04%), Postives = 276/436 (63.30%), Query Frame = 0

Query: 23  NDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIETWIEGGNFSCPFTNQPLQSIE 82
           ++ VE+TIPS F+CPIS +LMKDPV +++GITYDR +IE W E G  +CP TN  L S+E
Sbjct: 26  SEEVEITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLE 85

Query: 83  SIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKMAAAARRGDCDGCKSM 142
            IPNH IR+MIQ WC  +   GIERIPTPRVP +  QV EI E+++AA RRGD   C  M
Sbjct: 86  QIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEM 145

Query: 143 VEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKPENLERSIEVLEEILSAMATMFPL 202
           V K+ +LGKESERN+KC+  NG G +L   F+ FS  EN   S+ +LEE +S +  M P+
Sbjct: 146 VTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFS--ENANASL-LLEETVSVLTWMLPI 205

Query: 203 QEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGALEGL 262
             E    L + +S   LV  ++ GD    +N+  ++KE++      V+ L +I G  E  
Sbjct: 206 GLEGQSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAF 265

Query: 263 VKLI-KDPICSSSKKASLFITYHLIASSTSSSSSSNQERFTKALLEMGLVSLLLETLVDA 322
           +K I +D  C +    SL   +H+I         +NQE  ++  LE+ LV++ +E LVD+
Sbjct: 266 MKSINRDSTCVN----SLISIHHMIL--------TNQETVSR-FLELDLVNITVEMLVDS 325

Query: 323 ERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKILRVSDLATELSVSIVWKLVKH 382
           E SVCE+AL   + ICETK+GRE+   + L +P++VKKIL++S+   +  VS++WK+ K 
Sbjct: 326 ENSVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCKS 385

Query: 383 ENREDEEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHR-----DR 442
            +  + E       EAL++GAF+KL+++LQVGC E TKEK TELLK++N + +     DR
Sbjct: 386 GDGSEVE-------EALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDR 431

Query: 443 LECIDSLDFKDLKRTF 453
                S++FK +K+ F
Sbjct: 446 -SYSSSIEFKHVKKPF 431

BLAST of ClCG07G005470 vs. TAIR 10
Match: AT1G66160.2 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 301.2 bits (770), Expect = 1.4e-81
Identity = 183/436 (41.97%), Postives = 263/436 (60.32%), Query Frame = 0

Query: 23  NDMVELTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIETWIEGGNFSCPFTNQPLQSIE 82
           ++ VE+TIPS F+CPIS +LMKDP                W E G  +CP TN  L S+E
Sbjct: 26  SEEVEITIPSQFQCPISYELMKDP----------------WFESGYQTCPVTNTVLTSLE 85

Query: 83  SIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKMAAAARRGDCDGCKSM 142
            IPNH IR+MIQ WC  +   GIERIPTPRVP +  QV EI E+++AA RRGD   C  M
Sbjct: 86  QIPNHTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEM 145

Query: 143 VEKIKKLGKESERNKKCIIGNGTGNILSSTFEVFSKPENLERSIEVLEEILSAMATMFPL 202
           V K+ +LGKESERN+KC+  NG G +L   F+ FS  EN   S+ +LEE +S +  M P+
Sbjct: 146 VTKMTRLGKESERNRKCVKENGAGLVLCVCFDAFS--ENANASL-LLEETVSVLTWMLPI 205

Query: 203 QEEAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGALEGL 262
             E    L + +S   LV  ++ GD    +N+  ++KE++      V+ L +I G  E  
Sbjct: 206 GLEGQSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAF 265

Query: 263 VKLI-KDPICSSSKKASLFITYHLIASSTSSSSSSNQERFTKALLEMGLVSLLLETLVDA 322
           +K I +D  C +    SL   +H+I         +NQE  ++  LE+ LV++ +E LVD+
Sbjct: 266 MKSINRDSTCVN----SLISIHHMIL--------TNQETVSR-FLELDLVNITVEMLVDS 325

Query: 323 ERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKILRVSDLATELSVSIVWKLVKH 382
           E SVCE+AL   + ICETK+GRE+   + L +P++VKKIL++S+   +  VS++WK+ K 
Sbjct: 326 ENSVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCKS 385

Query: 383 ENREDEEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHR-----DR 442
            +  + E       EAL++GAF+KL+++LQVGC E TKEK TELLK++N + +     DR
Sbjct: 386 GDGSEVE-------EALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDR 415

Query: 443 LECIDSLDFKDLKRTF 453
                S++FK +K+ F
Sbjct: 446 -SYSSSIEFKHVKKPF 415

BLAST of ClCG07G005470 vs. TAIR 10
Match: AT3G02840.1 (ARM repeat superfamily protein )

HSP 1 Score: 251.9 bits (642), Expect = 9.7e-67
Identity = 151/353 (42.78%), Postives = 227/353 (64.31%), Query Frame = 0

Query: 105 IERIPTPRVPASPAQVREILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIGNG 164
           IERI +PRV  +P    EI  ++  AA R +   C  +V KIK LG+  + NKKC++ NG
Sbjct: 40  IERIQSPRVLLTPRDAVEISRRLQNAAAREEYAECLEIVSKIKNLGRGGDTNKKCLVQNG 99

Query: 165 TGNILSSTFEVFSKPENLERSIEVLEEILSAMATMFPL-QEEAIKHLKSETSLQSLVWFV 224
           +   LSS FE F+     +  + +LEEIL  +++  PL + E    + S  SL  LV F+
Sbjct: 100 SVLALSSCFERFAAAR--DGHMRLLEEILFVLSSWLPLNRSEGFNKMGSTASLNCLVRFL 159

Query: 225 KGGDISGRRNSVLVLKEIISSYPEKVNELGEIQGALEGLVKLIKDPICSSSKKASLFITY 284
            G D   R+N+   ++E+I+     V  L +++GA EGLVK+I+D + +SS KASL + Y
Sbjct: 160 NGKDAKTRQNAAFCIREVIAVDKRYVYALTDVEGACEGLVKIIRDSVSTSSTKASLMVIY 219

Query: 285 HLIASSTSSSSSSNQERFTKALLEMGLVSLLLETLV-DAERSVCERALGAFDGICETKQG 344
             I+S+         ++ T+  +++GLV L+ E +V +AE+SVCER+L   +  C+ +QG
Sbjct: 220 RAISSN---------DKITEKFVKLGLVELITEMMVNNAEKSVCERSLVVLNATCDNEQG 279

Query: 345 REEAYGHALTMPVIVKKILRVSDLATELSVSIVWKLVKHENREDEEKEGGVKVEALQVGA 404
           +E+   +AL +P++VKKILRVSDLAT+ SVSI+WKL K  N++D E +  + VEALQVGA
Sbjct: 280 KEDVLRNALIVPLLVKKILRVSDLATQCSVSILWKLWK--NKKDGECDDRLLVEALQVGA 339

Query: 405 FQKLLLLLQVGCSEWTKEKATELLKLLNNLHR--DRLECID-SLDFKDLKRTF 453
           F+KLL+LLQVGC + TKEKA+ELL+ LN      ++  C+D S+  K++K++F
Sbjct: 340 FEKLLVLLQVGCEDKTKEKASELLRNLNRCRNEIEKTNCVDSSMHLKNVKKSF 379

BLAST of ClCG07G005470 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 207.2 bits (526), Expect = 2.7e-53
Identity = 150/428 (35.05%), Postives = 228/428 (53.27%), Query Frame = 0

Query: 28  LTIPSHFRCPISLDLMKDPVTLSTGITYDRGSIETWIE-GGNFSCPFTNQPLQSIESIPN 87
           + IP HFRCPISL+LM+DPVT+ TG TYDR SIE+W+  G N +CP T  PL     IPN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 88  HNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKMAAAARRGDCDGCKSMVEKI 147
           H +R++IQ+WCV NR+ G+ERIPTP+ PA P  VR +L +  A+A  G     +S    +
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQ--ASAITGTHVSVRSRAAAL 131

Query: 148 KKL---GKESERNKKCIIGNGTGNILSSTFEVFSKPENLERSIEVLEEILSAMATMFPLQ 207
           ++L    ++S++N+  I  +    IL     +FS+      S E++ E L A+  M P+ 
Sbjct: 132 RRLRGFARDSDKNRVLIAAHNATEILIKI--LFSE----TTSSELVSESL-ALLVMLPIT 191

Query: 208 E--EAIKHLKSETSLQSLVWFVKGGDISGRRNSVLVLKEIISSYPEKVNELGEI---QGA 267
           E  + +        ++ L   +    I  R N+  ++ EI+S+  +  +  G I   +  
Sbjct: 192 EPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALI-EIVSTGTKSADLKGSISNSESV 251

Query: 268 LEGLVKLIKDPICSSSKKASLFITYHLIASSTSSSSSSNQERFTKALLEMGLVSLLLETL 327
            EG++ L+++PI   S + +L I    + +  S  S+ +        +  G   +L++ L
Sbjct: 252 FEGVLDLLRNPI---SSRRALKIGIKTLFALCSVKSTRH------IAITAGAPEILIDRL 311

Query: 328 -VDAERSVCERALGAFDGICETKQGREEAYGHALTMPVIVKKILRVSDLATELSVSIVWK 387
             D +R   ERAL   + +C T +G      HALT+P++VK ILRVSD ATE +   +  
Sbjct: 312 AADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLA 371

Query: 388 LVKHENREDEEKEGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRL 446
           L   E R  EE  G         G   +LLL++Q  C+E  K+KA +LLKLL +   D  
Sbjct: 372 LCTAEERWREEAAG--------AGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYN 412

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038892848.11.7e-23091.37U-box domain-containing protein 21-like [Benincasa hispida][more]
XP_004142305.12.8e-22589.33U-box domain-containing protein 21 [Cucumis sativus] >KGN49607.1 hypothetical pr... [more]
XP_008465536.15.1e-21987.56PREDICTED: U-box domain-containing protein 21-like [Cucumis melo][more]
KAA0056826.19.3e-21388.22U-box domain-containing protein 21-like [Cucumis melo var. makuwa] >TYJ99948.1 U... [more]
XP_022996865.11.0e-21183.85U-box domain-containing protein 21-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q5PNY62.1e-10349.18U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2... [more]
Q9C8D13.0e-8944.04U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1... [more]
Q9LT793.8e-5235.05U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FXA42.1e-5033.33U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Q84TG32.0e-4830.81E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KMI81.3e-22589.33RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G023870 PE... [more]
A0A1S3CPH42.5e-21987.56RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103503164 PE=4 ... [more]
A0A5D3BPA44.5e-21388.22RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1K7Y65.0e-21283.85RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491979 P... [more]
A0A6J1FPJ61.6e-21083.48RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447642... [more]
Match NameE-valueIdentityDescription
AT5G37490.11.5e-10449.18ARM repeat superfamily protein [more]
AT1G66160.12.1e-9044.04CYS, MET, PRO, and GLY protein 1 [more]
AT1G66160.21.4e-8141.97CYS, MET, PRO, and GLY protein 1 [more]
AT3G02840.19.7e-6742.78ARM repeat superfamily protein [more]
AT3G19380.12.7e-5335.05plant U-box 25 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 33..96
e-value: 1.6E-27
score: 107.4
IPR003613U box domainPFAMPF04564U-boxcoord: 30..101
e-value: 7.0E-20
score: 71.0
IPR003613U box domainPROSITEPS51698U_BOXcoord: 29..103
score: 39.710114
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 4..106
e-value: 1.2E-27
score: 97.8
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 118..431
e-value: 4.9E-15
score: 57.4
NoneNo IPR availablePANTHERPTHR22849:SF139ARM REPEAT SUPERFAMILY PROTEINcoord: 13..452
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 13..452
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 34..76
e-value: 3.73991E-23
score: 89.6002
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 23..106
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 184..429

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG07G005470.1ClCG07G005470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity