ClCG07G004770 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG07G004770
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Descriptionalpha-soluble NSF attachment protein 2-like
LocationCG_Chr07: 6129204 .. 6132641 (-)
RNA-Seq ExpressionClCG07G004770
SyntenyClCG07G004770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGATCATTTGGCCAGAGCAGAGGATTTCGAAGCCAAAGCAGAAAAGAAGCTCAGTGGTTGGGGATTGTTCGGCTCCAAGTACGATGACGCCGCAGATCTCTTCGATAAAGCCGCCAACTCTTACAAGCTTTCCAAATCATGTACTGCTAATCGCTTTTTCTCCTGATTTTTTGTTTCCCTTTGTTTGATCATTACTTTTCTTGTTATTGTTATTTTCATTTCCCCTAAACACTGCTGCATATTAGGAGCTCTTAATTTTGTTGTATGTTTTGATCATTTTGATGAGTAATTTAATTGTGTCATATCCCTTTTTGGTTCTTTTCTGATATGATTTCTCTCGTTTTTCTGACAATGGTTTTGGGTAATGCGAGAATTTTTGCTTGGAAATCAAGAGTTTGGTGGTTTTAACTTTTGATTGATCGGCATTGTTGCATTCTTGTTTGGGGCGGAGCCAGAAATTTTCATGATGAGGAGAAAAGTTCTATTCCTATATATAATCTTTGGAGCTTTAGAAATTTAGGAGGTAAACAGAAGAGAAAGTCAAAACGATAAACTAAGAGGAGCATTGCGTTGTGGCCGATGCGTTGTGGCCGTCATCTTAATTGGTCATTGCCTTGTTACATTAGCTGTGTTCTTGCTTGAATGAATAAAACATTGTTGTCTATTTTAGTTTACTGCCATCCATTGGCTTTCTTATCTCTGTGGATGTGGTTTTGTAGGGGATAAAGCAGGATCAACATATATAAAGTTGGCAAATTGTCATCTGAAGGTTGGTGCTTCGTGTTTGTGAGATCGAAGGCTTCATCTTCCTTTATTTTATACTCTTTTACTGAAAAATGCTTTGTTTTTATTGTAAAGATGGAAAGCAAACATGAAGCAGCTCAAGCTTATGTCGATGCGGGCAATTGCTATAAGAAAACATCTGTTAGAGGTAGTTCTGGTTCATAATATGATTGTTTAATGTGAAAAAAAATGGTTAATGTGAAGTCCATTCAGGGACATTTGATACGGTAATGTGATTTATATGAGTGAAACAATGTTAGTTCGAGTATGACATTATTGTTGGTTAAATTACTTATTTCCAAATTCCAATTGTTATTTTGATGCAGAGGCCATATCTTGCATAGAGCAAGCAGTGAATTTGTTTTGTGACATTGGAAGACTCAATATGGCTGCAAGATATTACAAGGTACATTCTTATTCTGGTACATGAAATGTTTATTGCTTGATTCCTTTGATCATCTAAAATGTTCGGTTACTCAGTGAGGTTATGCTCTGAAATTTTTGTTCTTTTCTTTTGGGGTTTATTCTTGTGGCATTAGGTCCATTTAGACTGTACTTTTCTTGCATAAAATTTTGGCAATTACTTAAGCAGAATTCTTTCATCATTTGCAGAGAATTAGCAAGTACCTCATGTCCAATTCATGACTAGGATTTGCCTTCTTGTGTGTGTAGGAAATTGCTGAATTGCACGAGTCGGAACAGGATATTGAGCAAGCAATTGATTACTTTGAAAAGGCTGCTGACCTTTTCCAAAATGAAGAAGTGACAACTTCAGCAAACCAATGCAAGCAGAAAGTTGCACAATTTGCTGCACAGTTAGAACAGTAAGATACTTTGACACATTTAGTTATTATGCTCTTTTTAGCAATTTCTTTTAGCATTGTTAGCATTGCTAGTTGCTACCACCAATCTAATGTTTGTAGTCTGTACATTCTTGTTTTCTTGTATTAACCTTCCATATGATAACATAATTTCCAGATATCAGAAAGCAATCGATATCTATGAAGAGATAGCAAAGTATTCTCTCAAGAACAACTTGCTCAAGTATGGAGTGAAAGGGCATCTTCTTAATGCTGGAATTTGCCAGCTTTGCAAAGGTGATATCGTTGCTATTACAAAAGCATTGGATAACTATCAGGTCTTTCCTCTACTCGACTCTCTTAGCTCCTGCCGAATTCTGCCCTGCCCACAGGCTGGGAAAATAGTTTGATGTCTTACTTCCCTTTGCAGGATTTGGATCCTACTTTTTCAGGGACACGTGAATACAAGCTTTTAGCGGTGTGTGACGTTCACCCCTGTATTCTGATATCTTATTCTTTCTTTTACATATTTCATAATATTTTATACGAGGCACAATGGTTTTCAGTGGGACAAAAAAGAGATTATTATTTCATTTCAATAGCTGCAAGACCAAAACAGGATGCAGAAGAGCGAAAGCCCTTCTTTTAACTTCCTCCCAGATACACGGTTTATTAATTTGATTCACAATGCCTAAAGTTTGAGCTACATATGAGTATCTCTCTGTTGCCATGTTAAATTTATGCTATATCCAAAAAAATTTAAAATAATGTTTTTAATGCTTTTTTTTTTTCATATATATTTGTTGAAGTTGAACAAGAAGTTCAATGGTTCTTCCCATTTCTGGCTTGCTATTGATTGAAGATATTGTCATGGATTGGCTGATTTGTACCATATCTTGCATAAAATTGTACTGCTTCTTGACTTAGGCAATCTCTCATCTTCTTTCTTTTCATGTTCCATTTTTTTTTTTCTCTCATATGCCTCAGGACATTGCATCTGCAATTGATGAGGAAGATGTAGCAAAATTTACAGATGTTGTCAAGGAATTTGATAGCATGACCACACTGGTAAGATTATATACTTAATATTATTGTTTCTGGGGAACTGTATAAAGCTAACATGATATTGTCAATGAGGGCCATGGGTCTAATAAGGAAATTGGTTCAATCTATGTTGCCTACCAGCTTAGGATTTAATATCCTACAAGATTTCCTGGCACCCAAATGTTGTAGAGTCAAGTGGGTTGGCTTGTGAAATTAGTTGCGGTGTGCGCAAACTTCTCGGACACTCATTGATATCAAGAAAGAAATAAGGAAAAAAAAAGTAACATGATATTGATATGTAAACTGCAAAGTTGCTTCCTAAAACTCATAGATCTAGTGAATCTTAGATATCGAACTCGGAATCACCTGCAAATAACATCAAAACACACACACAACAGAGGGAAACAAGTACCAGAGGCTGTTATTTTGATAATTCACATATAGTGAGATATCCAGCACAATCACAACCCAGACGATACGTCCGAGAACAAGAAAACACAAGTCGAGAGAGCTTGGAACCTCTCGTACTGACCCCTGCAAACCCCCACTCATCAACCATACACTCACGTTTCTTACCCATCCTAAAGTAGGTTTGCACAATCGGAGGCCTCACACAGAAATGTAGTTTGATCATTTAGAAACGACCAAAAGGAATCTTAGCATTCTAACTATATTGTTTTCATTTCTAATGGTGGAATTACTAATGAATAATGTGCATTTGACAGGATCCATTGAAGACAACCCTTCTCTTAAGGGTGAAGGATAAACTGAAAGCTAAAGAGCTGGAAGAGGATGATCTTACCTAA

mRNA sequence

ATGGCGGATCATTTGGCCAGAGCAGAGGATTTCGAAGCCAAAGCAGAAAAGAAGCTCAGTGGTTGGGGATTGTTCGGCTCCAAGTACGATGACGCCGCAGATCTCTTCGATAAAGCCGCCAACTCTTACAAGCTTTCCAAATCATGGGATAAAGCAGGATCAACATATATAAAGTTGGCAAATTGTCATCTGAAGATGGAAAGCAAACATGAAGCAGCTCAAGCTTATGTCGATGCGGGCAATTGCTATAAGAAAACATCTGTTAGAGAGGCCATATCTTGCATAGAGCAAGCAGTGAATTTGTTTTGTGACATTGGAAGACTCAATATGGCTGCAAGATATTACAAGGAAATTGCTGAATTGCACGAGTCGGAACAGGATATTGAGCAAGCAATTGATTACTTTGAAAAGGCTGCTGACCTTTTCCAAAATGAAGAAGTGACAACTTCAGCAAACCAATGCAAGCAGAAAGTTGCACAATTTGCTGCACAGTTAGAACAATATCAGAAAGCAATCGATATCTATGAAGAGATAGCAAAGTATTCTCTCAAGAACAACTTGCTCAAGTATGGAGTGAAAGGGCATCTTCTTAATGCTGGAATTTGCCAGCTTTGCAAAGGTGATATCGTTGCTATTACAAAAGCATTGGATAACTATCAGGATTTGGATCCTACTTTTTCAGGGACACGTGAATACAAGCTTTTAGCGGTGTGTGACGACATTGCATCTGCAATTGATGAGGAAGATGTAGCAAAATTTACAGATGTTGTCAAGGAATTTGATAGCATGACCACACTGGATCCATTGAAGACAACCCTTCTCTTAAGGGTGAAGGATAAACTGAAAGCTAAAGAGCTGGAAGAGGATGATCTTACCTAA

Coding sequence (CDS)

ATGGCGGATCATTTGGCCAGAGCAGAGGATTTCGAAGCCAAAGCAGAAAAGAAGCTCAGTGGTTGGGGATTGTTCGGCTCCAAGTACGATGACGCCGCAGATCTCTTCGATAAAGCCGCCAACTCTTACAAGCTTTCCAAATCATGGGATAAAGCAGGATCAACATATATAAAGTTGGCAAATTGTCATCTGAAGATGGAAAGCAAACATGAAGCAGCTCAAGCTTATGTCGATGCGGGCAATTGCTATAAGAAAACATCTGTTAGAGAGGCCATATCTTGCATAGAGCAAGCAGTGAATTTGTTTTGTGACATTGGAAGACTCAATATGGCTGCAAGATATTACAAGGAAATTGCTGAATTGCACGAGTCGGAACAGGATATTGAGCAAGCAATTGATTACTTTGAAAAGGCTGCTGACCTTTTCCAAAATGAAGAAGTGACAACTTCAGCAAACCAATGCAAGCAGAAAGTTGCACAATTTGCTGCACAGTTAGAACAATATCAGAAAGCAATCGATATCTATGAAGAGATAGCAAAGTATTCTCTCAAGAACAACTTGCTCAAGTATGGAGTGAAAGGGCATCTTCTTAATGCTGGAATTTGCCAGCTTTGCAAAGGTGATATCGTTGCTATTACAAAAGCATTGGATAACTATCAGGATTTGGATCCTACTTTTTCAGGGACACGTGAATACAAGCTTTTAGCGGTGTGTGACGACATTGCATCTGCAATTGATGAGGAAGATGTAGCAAAATTTACAGATGTTGTCAAGGAATTTGATAGCATGACCACACTGGATCCATTGAAGACAACCCTTCTCTTAAGGGTGAAGGATAAACTGAAAGCTAAAGAGCTGGAAGAGGATGATCTTACCTAA

Protein sequence

MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLANCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAELHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAKYSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDDIASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT
Homology
BLAST of ClCG07G004770 vs. NCBI nr
Match: XP_038893428.1 (alpha-soluble NSF attachment protein 2-like [Benincasa hispida])

HSP 1 Score: 557.4 bits (1435), Expect = 7.4e-155
Identity = 288/292 (98.63%), Postives = 289/292 (98.97%), Query Frame = 0

Query: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA
Sbjct: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE
Sbjct: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120

Query: 121 LHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+ESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK
Sbjct: 121 LYESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180

Query: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDD 240
           YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLA   D
Sbjct: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLA---D 240

Query: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 293
           IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT
Sbjct: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 289

BLAST of ClCG07G004770 vs. NCBI nr
Match: XP_022956354.1 (alpha-soluble NSF attachment protein 2-like [Cucurbita moschata] >XP_023536437.1 alpha-soluble NSF attachment protein 2-like [Cucurbita pepo subsp. pepo] >KAG6600896.1 hypothetical protein SDJN03_06129, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031530.1 hypothetical protein SDJN02_05570 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 550.1 bits (1416), Expect = 1.2e-152
Identity = 283/292 (96.92%), Postives = 288/292 (98.63%), Query Frame = 0

Query: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MADHLARAEDF++KAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA
Sbjct: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLKMESKHEAAQAY+DAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE
Sbjct: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120

Query: 121 LHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L ESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK
Sbjct: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180

Query: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDD 240
           YSLK+NLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSG+REYKLLA   D
Sbjct: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGSREYKLLA---D 240

Query: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 293
           IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT
Sbjct: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 289

BLAST of ClCG07G004770 vs. NCBI nr
Match: XP_022991930.1 (alpha-soluble NSF attachment protein 2-like [Cucurbita maxima])

HSP 1 Score: 548.9 bits (1413), Expect = 2.6e-152
Identity = 282/292 (96.58%), Postives = 288/292 (98.63%), Query Frame = 0

Query: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MADHLARAEDF++KAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA
Sbjct: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLKMESKHEAAQAY+DAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE
Sbjct: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120

Query: 121 LHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L ESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK
Sbjct: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180

Query: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDD 240
           YSLK+NLLKYGVKGHLLNAGICQLCKGDIVAIT+ALDNYQDLDPTFSG+REYKLLA   D
Sbjct: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLA---D 240

Query: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 293
           IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT
Sbjct: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 289

BLAST of ClCG07G004770 vs. NCBI nr
Match: XP_022146873.1 (alpha-soluble NSF attachment protein 2-like [Momordica charantia])

HSP 1 Score: 546.6 bits (1407), Expect = 1.3e-151
Identity = 279/292 (95.55%), Postives = 288/292 (98.63%), Query Frame = 0

Query: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MADHLARA+DFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA
Sbjct: 1   MADHLARADDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLKMESKHEAAQAYVDAGNCYKKTSV+EAISCIEQAVNLFCDIGRLNMAARYYKEIAE
Sbjct: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVKEAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120

Query: 121 LHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+E+EQD+E+AIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK
Sbjct: 121 LYENEQDVERAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180

Query: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDD 240
           YSLKNNLLKYG KGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSG+REYKLLA   D
Sbjct: 181 YSLKNNLLKYGAKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGSREYKLLA---D 240

Query: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 293
           IASA+DEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVK+KLKAKELEEDDLT
Sbjct: 241 IASALDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKEKLKAKELEEDDLT 289

BLAST of ClCG07G004770 vs. NCBI nr
Match: KAF3964963.1 (hypothetical protein CMV_010801 [Castanea mollissima])

HSP 1 Score: 492.7 bits (1267), Expect = 2.3e-135
Identity = 249/292 (85.27%), Postives = 273/292 (93.49%), Query Frame = 0

Query: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           M D +ARAEDFE KAEKKLSGWGLFGSKY+DAADLFDKAANS+KL KSWDKAGSTY+KLA
Sbjct: 1   MGDQIARAEDFEKKAEKKLSGWGLFGSKYEDAADLFDKAANSFKLGKSWDKAGSTYVKLA 60

Query: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLK+ESKHEAAQAYVDA +CYKK+S+ EAISC+EQAVN+FC+IGRL+MAARY+KEIAE
Sbjct: 61  NCHLKLESKHEAAQAYVDAAHCYKKSSINEAISCLEQAVNMFCEIGRLSMAARYFKEIAE 120

Query: 121 LHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+ESE +IEQAI YFEK+AD FQNEEVTTSANQCKQKVAQFAAQLEQYQK+I+IYEEIA+
Sbjct: 121 LYESEHNIEQAIVYFEKSADFFQNEEVTTSANQCKQKVAQFAAQLEQYQKSIEIYEEIAR 180

Query: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDD 240
            SL NNLLKYGVKGHLLNAGICQLCKGD+VAIT AL+ YQ+LDPTFSGTREYKLLA   D
Sbjct: 181 QSLNNNLLKYGVKGHLLNAGICQLCKGDVVAITNALERYQELDPTFSGTREYKLLA---D 240

Query: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 293
           IASA+DEEDVAKFTDVVKEFDSMT LD  KTTLLLRVK+KLKAKELEEDDLT
Sbjct: 241 IASALDEEDVAKFTDVVKEFDSMTPLDSWKTTLLLRVKEKLKAKELEEDDLT 289

BLAST of ClCG07G004770 vs. ExPASy Swiss-Prot
Match: Q9SPE6 (Alpha-soluble NSF attachment protein 2 OS=Arabidopsis thaliana OX=3702 GN=ASNAP2 PE=1 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 5.4e-124
Identity = 221/292 (75.68%), Postives = 254/292 (86.99%), Query Frame = 0

Query: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           M DHL RAE+FE KAEKKL+GWG+FGSKY+DAADL +KAANSYKL+KSWD+AG  Y+KLA
Sbjct: 1   MGDHLVRAEEFEKKAEKKLNGWGIFGSKYEDAADLLEKAANSYKLAKSWDQAGKAYLKLA 60

Query: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           +CHLK +SKH+AA AY +A  CYKK    EA SC+E+AVN+FC+IGRLNMAARYYKEIAE
Sbjct: 61  DCHLKSDSKHDAANAYAEAAKCYKKVDTNEAASCLERAVNIFCEIGRLNMAARYYKEIAE 120

Query: 121 LHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
            +ES+Q  EQAI YFEKAA+ FQNEEVTTSANQC  KVAQ+AAQLEQY+KAI IYE+IA+
Sbjct: 121 YYESDQKFEQAIAYFEKAAEFFQNEEVTTSANQCNLKVAQYAAQLEQYEKAIKIYEDIAR 180

Query: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDD 240
           +SL NNLLKYGVKGHLL AG+C LCK D+V+IT AL+ YQDLDPTF+GTRE K LA   D
Sbjct: 181 HSLNNNLLKYGVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFTGTRECKFLA---D 240

Query: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 293
           +ASAIDEED+AKFTDVVKEFDSMT LD  KTT+LLRVK+KLKAKELEEDDLT
Sbjct: 241 LASAIDEEDIAKFTDVVKEFDSMTPLDSWKTTMLLRVKEKLKAKELEEDDLT 289

BLAST of ClCG07G004770 vs. ExPASy Swiss-Prot
Match: P93798 (Alpha-soluble NSF attachment protein OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 3.3e-121
Identity = 219/292 (75.00%), Postives = 251/292 (85.96%), Query Frame = 0

Query: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MAD++ R E+FE KAEKK+SGWGLFGSKY+DAAD +DKAAN +KL+KSWD+AGSTY+KL+
Sbjct: 1   MADNIQRGEEFEKKAEKKISGWGLFGSKYEDAADFYDKAANCFKLAKSWDRAGSTYVKLS 60

Query: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
                 +SKHEAAQAY DAG+CYKKTS +EAISC+EQA  LF D GR NMA +YYKEIAE
Sbjct: 61  TVIQSSDSKHEAAQAYADAGHCYKKTSAKEAISCLEQAAYLFLDNGRFNMAGKYYKEIAE 120

Query: 121 LHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+E EQ+ EQAI YFEKAAD++Q+EE TT+ANQC  KVAQFAAQLEQYQKAI IYE+I +
Sbjct: 121 LYELEQNFEQAIIYFEKAADIYQSEEATTAANQCNAKVAQFAAQLEQYQKAIQIYEDIGR 180

Query: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDD 240
            SL NNLLKYGVKGHLLNAGICQLCKGD+VAIT ALD YQ++DPTFSGTREYKLL    D
Sbjct: 181 PSLNNNLLKYGVKGHLLNAGICQLCKGDVVAITNALDRYQEMDPTFSGTREYKLLV---D 240

Query: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 293
           +A+A+DEEDV KFTD VKEFDSMT LD  KTTLLLRVK+ +KAKELEEDDLT
Sbjct: 241 LAAAVDEEDVVKFTDAVKEFDSMTQLDAWKTTLLLRVKEAIKAKELEEDDLT 289

BLAST of ClCG07G004770 vs. ExPASy Swiss-Prot
Match: Q9M5P8 (Alpha-soluble NSF attachment protein OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 5.6e-113
Identity = 205/292 (70.21%), Postives = 247/292 (84.59%), Query Frame = 0

Query: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           M DH+AR EDFE KAEKKL GW LFGSKY+DAADLFDKA N +KL+KSWDKAG+ Y+KLA
Sbjct: 1   MGDHIARGEDFENKAEKKLGGWALFGSKYEDAADLFDKAGNCFKLAKSWDKAGAVYVKLA 60

Query: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLK++SKHEAA AY DA +CYKK++++EA+SC+EQAVN F DIGRLNM+ARYYKEIAE
Sbjct: 61  NCHLKLDSKHEAANAYADAAHCYKKSNIKEAVSCLEQAVNFFLDIGRLNMSARYYKEIAE 120

Query: 121 LHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+E EQ+++QAI Y     +   +E+V+   NQC +K+AQF+AQ E+Y KAI+I+EEIA+
Sbjct: 121 LYEQEQNLDQAIRYAVYEINP-GSEDVSAPPNQCLKKIAQFSAQNEKYPKAIEIFEEIAR 180

Query: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDD 240
           +S+ NNLLKYGV+ HLLNAGICQLCKGD+V I  AL+ YQ+LDPTFSGTRE KLL    D
Sbjct: 181 HSVNNNLLKYGVREHLLNAGICQLCKGDVVPINNALERYQELDPTFSGTRECKLLV---D 240

Query: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 293
           +A+AIDEEDVAKFTD VKE+DSMT LD  +TTLLLRVK+ LKAKELEEDDLT
Sbjct: 241 LAAAIDEEDVAKFTDAVKEYDSMTQLDAWRTTLLLRVKETLKAKELEEDDLT 288

BLAST of ClCG07G004770 vs. ExPASy Swiss-Prot
Match: Q9LXZ5 (Alpha-soluble NSF attachment protein 1 OS=Arabidopsis thaliana OX=3702 GN=ASNAP1 PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 6.5e-61
Identity = 137/256 (53.52%), Postives = 167/256 (65.23%), Query Frame = 0

Query: 11  FEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLANCHLKMESKH 70
           F+ KAEKKL+    F +KY+DAADL +KA +SYKL+KSWD+AG  Y+KLA+CHLK  S  
Sbjct: 99  FQKKAEKKLNRRRRFDTKYEDAADLLEKARDSYKLAKSWDQAGIAYLKLADCHLKANS-- 158

Query: 71  EAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAELHESEQDIEQ 130
             A  Y+    C                                  EIAE +ES++  EQ
Sbjct: 159 -LANTYMIMDECMD-------------------------------HEIAEYYESDEMFEQ 218

Query: 131 AIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAKYSLKNNLLKY 190
           AI Y+E AA+ FQ EEVTTSANQC  KVAQ+A+QLEQ  +     +  A++SL N LLKY
Sbjct: 219 AIAYYETAAEFFQIEEVTTSANQCNLKVAQYASQLEQQSR---FMKTQARHSLNNKLLKY 278

Query: 191 GVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDDIASAIDEEDV 250
           GVKGHLL AG+C LCK D+V+IT AL+ YQDLDPTFSGTRE K LA   D+ASAIDEED+
Sbjct: 279 GVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFSGTRECKFLA---DLASAIDEEDI 314

Query: 251 AKFTDVVKEFDSMTTL 267
           AKFTDV KE DS++ L
Sbjct: 339 AKFTDVSKEIDSVSPL 314

BLAST of ClCG07G004770 vs. ExPASy Swiss-Prot
Match: Q54NP6 (Alpha-soluble NSF attachment protein OS=Dictyostelium discoideum OX=44689 GN=snpA PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.9e-60
Identity = 126/282 (44.68%), Postives = 188/282 (66.67%), Query Frame = 0

Query: 6   ARAEDFEAKAEKKLSGWGLF------GSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKL 65
           A+A++F   A+K+L G   F       S+YDDAA  + KAAN +K+SK WD+AG+ + K 
Sbjct: 5   AKAKEFLDAADKRLRGGNFFKMFGGGSSRYDDAASDYTKAANLFKMSKKWDQAGAAFQKA 64

Query: 66  ANCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIA 125
           A C LK  SKH+AA +YV A  CYKK +V +AI+C++ A+  + D GR  ++A++ KEIA
Sbjct: 65  AECFLKGSSKHDAASSYVLAAGCYKKGNVIDAITCLKAAIEYYTDEGRFAISAKHQKEIA 124

Query: 126 ELHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIA 185
           EL+E+E D +QAI  ++ A+D F  E  T S++QC  K+A F+AQLE+Y+K+I+IYE++A
Sbjct: 125 ELYEAEGDFDQAIASYQIASDYFDGENSTVSSHQCLLKIALFSAQLERYEKSIEIYEQVA 184

Query: 186 KYSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCD 245
             SL NNL ++G K + L A +C L   D+V   +AL  Y+D+  +F+ TRE +LL   D
Sbjct: 185 AASLDNNLTQWGCKEYFLRACLCYLAADDVVGAERALQRYKDMQASFNSTRECRLL---D 244

Query: 246 DIASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKL 282
            I  A    +V  FT+ V EF+S++ LD  KT++LLR+K+ +
Sbjct: 245 GIIQACRNNNVEDFTNEVAEFNSISPLDSWKTSILLRIKNTI 283

BLAST of ClCG07G004770 vs. ExPASy TrEMBL
Match: A0A6J1GYT0 (alpha-soluble NSF attachment protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111458118 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 5.8e-153
Identity = 283/292 (96.92%), Postives = 288/292 (98.63%), Query Frame = 0

Query: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MADHLARAEDF++KAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA
Sbjct: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLKMESKHEAAQAY+DAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE
Sbjct: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120

Query: 121 LHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L ESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK
Sbjct: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180

Query: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDD 240
           YSLK+NLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSG+REYKLLA   D
Sbjct: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGSREYKLLA---D 240

Query: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 293
           IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT
Sbjct: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 289

BLAST of ClCG07G004770 vs. ExPASy TrEMBL
Match: A0A6J1JUA6 (alpha-soluble NSF attachment protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488422 PE=3 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 1.3e-152
Identity = 282/292 (96.58%), Postives = 288/292 (98.63%), Query Frame = 0

Query: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MADHLARAEDF++KAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA
Sbjct: 1   MADHLARAEDFDSKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLKMESKHEAAQAY+DAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE
Sbjct: 61  NCHLKMESKHEAAQAYIDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120

Query: 121 LHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L ESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK
Sbjct: 121 LFESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180

Query: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDD 240
           YSLK+NLLKYGVKGHLLNAGICQLCKGDIVAIT+ALDNYQDLDPTFSG+REYKLLA   D
Sbjct: 181 YSLKSNLLKYGVKGHLLNAGICQLCKGDIVAITRALDNYQDLDPTFSGSREYKLLA---D 240

Query: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 293
           IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT
Sbjct: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 289

BLAST of ClCG07G004770 vs. ExPASy TrEMBL
Match: A0A6J1CZQ2 (alpha-soluble NSF attachment protein 2-like OS=Momordica charantia OX=3673 GN=LOC111015967 PE=3 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 6.4e-152
Identity = 279/292 (95.55%), Postives = 288/292 (98.63%), Query Frame = 0

Query: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           MADHLARA+DFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA
Sbjct: 1   MADHLARADDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLKMESKHEAAQAYVDAGNCYKKTSV+EAISCIEQAVNLFCDIGRLNMAARYYKEIAE
Sbjct: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVKEAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120

Query: 121 LHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+E+EQD+E+AIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK
Sbjct: 121 LYENEQDVERAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180

Query: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDD 240
           YSLKNNLLKYG KGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSG+REYKLLA   D
Sbjct: 181 YSLKNNLLKYGAKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGSREYKLLA---D 240

Query: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 293
           IASA+DEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVK+KLKAKELEEDDLT
Sbjct: 241 IASALDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKEKLKAKELEEDDLT 289

BLAST of ClCG07G004770 vs. ExPASy TrEMBL
Match: A0A7N2R9Y3 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 5.4e-135
Identity = 248/292 (84.93%), Postives = 273/292 (93.49%), Query Frame = 0

Query: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           M D +ARAEDFE KAEKKLSGWGLFGSKY+DAADLFDKAANS+KL KSWDKAG+TY+KLA
Sbjct: 1   MGDQIARAEDFEKKAEKKLSGWGLFGSKYEDAADLFDKAANSFKLGKSWDKAGATYVKLA 60

Query: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLK+ESKHEAAQAYVDA +CYKK+++ EAISC+EQAVN+FC+IGRL+MAARY+KEIAE
Sbjct: 61  NCHLKLESKHEAAQAYVDAAHCYKKSNINEAISCLEQAVNMFCEIGRLSMAARYFKEIAE 120

Query: 121 LHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+ESEQ+IEQAI YFEK+AD FQNEEVTTSANQCKQKVAQFAAQLEQYQK+I IYEEIA+
Sbjct: 121 LYESEQNIEQAIVYFEKSADFFQNEEVTTSANQCKQKVAQFAAQLEQYQKSIVIYEEIAR 180

Query: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDD 240
            SL NNLLKYGVKGHLLNAGICQLCKGD+VAIT AL+ YQ+LDPTFSGTREYKLLA   D
Sbjct: 181 QSLNNNLLKYGVKGHLLNAGICQLCKGDVVAITNALERYQELDPTFSGTREYKLLA---D 240

Query: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 293
           IASA+DEEDVAKFTDVVKEFDSMT LD  KTTLLLRVK+KLKAKELEEDDLT
Sbjct: 241 IASALDEEDVAKFTDVVKEFDSMTPLDSWKTTLLLRVKEKLKAKELEEDDLT 289

BLAST of ClCG07G004770 vs. ExPASy TrEMBL
Match: A0A2P5E0M8 (NSF attachment protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_015780 PE=3 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.1e-134
Identity = 246/292 (84.25%), Postives = 274/292 (93.84%), Query Frame = 0

Query: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           M D +A+AE+FE KAEKKL+GWGLFGSKY+DAADLFDKAANS+KL+KSWD+AGSTYIKLA
Sbjct: 1   MGDQIAKAEEFEKKAEKKLNGWGLFGSKYEDAADLFDKAANSFKLAKSWDRAGSTYIKLA 60

Query: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           NCHLK+ESKHEAAQAYVDA +CYKKTSV EAISC+E+AV+LFCDIGRLNMAARY+KEIAE
Sbjct: 61  NCHLKLESKHEAAQAYVDAAHCYKKTSVNEAISCLEKAVHLFCDIGRLNMAARYFKEIAE 120

Query: 121 LHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
           L+ESEQ+IE+AI++FEKAAD FQNEEVTTSANQCKQKVAQFAAQLEQYQK+I+IYEEIA+
Sbjct: 121 LYESEQNIEKAIEFFEKAADFFQNEEVTTSANQCKQKVAQFAAQLEQYQKSIEIYEEIAR 180

Query: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDD 240
            SL NNLLKYGVKGHLLNAGIC LC+GD+VAIT AL+ YQDLDPTFSGTREYK LA   D
Sbjct: 181 QSLNNNLLKYGVKGHLLNAGICHLCRGDVVAITNALERYQDLDPTFSGTREYKFLA---D 240

Query: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 293
           IA++IDEEDVAKFTDVVKEFDSMT LD  KTTLLLRVK+KLKAKELEEDDLT
Sbjct: 241 IAASIDEEDVAKFTDVVKEFDSMTPLDSWKTTLLLRVKEKLKAKELEEDDLT 289

BLAST of ClCG07G004770 vs. TAIR 10
Match: AT3G56190.1 (alpha-soluble NSF attachment protein 2 )

HSP 1 Score: 445.3 bits (1144), Expect = 3.8e-125
Identity = 221/292 (75.68%), Postives = 254/292 (86.99%), Query Frame = 0

Query: 1   MADHLARAEDFEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLA 60
           M DHL RAE+FE KAEKKL+GWG+FGSKY+DAADL +KAANSYKL+KSWD+AG  Y+KLA
Sbjct: 1   MGDHLVRAEEFEKKAEKKLNGWGIFGSKYEDAADLLEKAANSYKLAKSWDQAGKAYLKLA 60

Query: 61  NCHLKMESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAE 120
           +CHLK +SKH+AA AY +A  CYKK    EA SC+E+AVN+FC+IGRLNMAARYYKEIAE
Sbjct: 61  DCHLKSDSKHDAANAYAEAAKCYKKVDTNEAASCLERAVNIFCEIGRLNMAARYYKEIAE 120

Query: 121 LHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAK 180
            +ES+Q  EQAI YFEKAA+ FQNEEVTTSANQC  KVAQ+AAQLEQY+KAI IYE+IA+
Sbjct: 121 YYESDQKFEQAIAYFEKAAEFFQNEEVTTSANQCNLKVAQYAAQLEQYEKAIKIYEDIAR 180

Query: 181 YSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDD 240
           +SL NNLLKYGVKGHLL AG+C LCK D+V+IT AL+ YQDLDPTF+GTRE K LA   D
Sbjct: 181 HSLNNNLLKYGVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFTGTRECKFLA---D 240

Query: 241 IASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDDLT 293
           +ASAIDEED+AKFTDVVKEFDSMT LD  KTT+LLRVK+KLKAKELEEDDLT
Sbjct: 241 LASAIDEEDIAKFTDVVKEFDSMTPLDSWKTTMLLRVKEKLKAKELEEDDLT 289

BLAST of ClCG07G004770 vs. TAIR 10
Match: AT3G56190.2 (alpha-soluble NSF attachment protein 2 )

HSP 1 Score: 339.7 bits (870), Expect = 2.3e-93
Identity = 176/242 (72.73%), Postives = 204/242 (84.30%), Query Frame = 0

Query: 53  GSTYIKLA-NCHLKM-ESKHEAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNM 112
           GS++  L+  C L + +SKH+AA AY +A  CYKK    EA SC+E+AVN+FC+IGRLNM
Sbjct: 2   GSSWKSLSKTCRLSLKDSKHDAANAYAEAAKCYKKVDTNEAASCLERAVNIFCEIGRLNM 61

Query: 113 AARYYKEIAELHESEQDIEQAIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQK 172
           AARYYKEIAE +ES+Q  EQAI YFEKAA+ FQNEEVTTSANQC  KVAQ+AAQLEQY+K
Sbjct: 62  AARYYKEIAEYYESDQKFEQAIAYFEKAAEFFQNEEVTTSANQCNLKVAQYAAQLEQYEK 121

Query: 173 AIDIYEEIAKYSLKNNLLKYGVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTR 232
           AI IYE+IA++SL NNLLKYGVKGHLL AG+C LCK D+V+IT AL+ YQDLDPTF+GTR
Sbjct: 122 AIKIYEDIARHSLNNNLLKYGVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFTGTR 181

Query: 233 EYKLLAVCDDIASAIDEEDVAKFTDVVKEFDSMTTLDPLKTTLLLRVKDKLKAKELEEDD 292
           E K LA   D+ASAIDEED+AKFTDVVKEFDSMT LD  KTT+LLRVK+KLKAKELEEDD
Sbjct: 182 ECKFLA---DLASAIDEEDIAKFTDVVKEFDSMTPLDSWKTTMLLRVKEKLKAKELEEDD 240

BLAST of ClCG07G004770 vs. TAIR 10
Match: AT3G56450.1 (alpha-soluble NSF attachment protein 1 )

HSP 1 Score: 235.7 bits (600), Expect = 4.6e-62
Identity = 137/256 (53.52%), Postives = 167/256 (65.23%), Query Frame = 0

Query: 11  FEAKAEKKLSGWGLFGSKYDDAADLFDKAANSYKLSKSWDKAGSTYIKLANCHLKMESKH 70
           F+ KAEKKL+    F +KY+DAADL +KA +SYKL+KSWD+AG  Y+KLA+CHLK  S  
Sbjct: 99  FQKKAEKKLNRRRRFDTKYEDAADLLEKARDSYKLAKSWDQAGIAYLKLADCHLKANS-- 158

Query: 71  EAAQAYVDAGNCYKKTSVREAISCIEQAVNLFCDIGRLNMAARYYKEIAELHESEQDIEQ 130
             A  Y+    C                                  EIAE +ES++  EQ
Sbjct: 159 -LANTYMIMDECMD-------------------------------HEIAEYYESDEMFEQ 218

Query: 131 AIDYFEKAADLFQNEEVTTSANQCKQKVAQFAAQLEQYQKAIDIYEEIAKYSLKNNLLKY 190
           AI Y+E AA+ FQ EEVTTSANQC  KVAQ+A+QLEQ  +     +  A++SL N LLKY
Sbjct: 219 AIAYYETAAEFFQIEEVTTSANQCNLKVAQYASQLEQQSR---FMKTQARHSLNNKLLKY 278

Query: 191 GVKGHLLNAGICQLCKGDIVAITKALDNYQDLDPTFSGTREYKLLAVCDDIASAIDEEDV 250
           GVKGHLL AG+C LCK D+V+IT AL+ YQDLDPTFSGTRE K LA   D+ASAIDEED+
Sbjct: 279 GVKGHLLTAGMCHLCKADVVSITNALEKYQDLDPTFSGTRECKFLA---DLASAIDEEDI 314

Query: 251 AKFTDVVKEFDSMTTL 267
           AKFTDV KE DS++ L
Sbjct: 339 AKFTDVSKEIDSVSPL 314

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893428.17.4e-15598.63alpha-soluble NSF attachment protein 2-like [Benincasa hispida][more]
XP_022956354.11.2e-15296.92alpha-soluble NSF attachment protein 2-like [Cucurbita moschata] >XP_023536437.1... [more]
XP_022991930.12.6e-15296.58alpha-soluble NSF attachment protein 2-like [Cucurbita maxima][more]
XP_022146873.11.3e-15195.55alpha-soluble NSF attachment protein 2-like [Momordica charantia][more]
KAF3964963.12.3e-13585.27hypothetical protein CMV_010801 [Castanea mollissima][more]
Match NameE-valueIdentityDescription
Q9SPE65.4e-12475.68Alpha-soluble NSF attachment protein 2 OS=Arabidopsis thaliana OX=3702 GN=ASNAP2... [more]
P937983.3e-12175.00Alpha-soluble NSF attachment protein OS=Vitis vinifera OX=29760 PE=2 SV=1[more]
Q9M5P85.6e-11370.21Alpha-soluble NSF attachment protein OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Q9LXZ56.5e-6153.52Alpha-soluble NSF attachment protein 1 OS=Arabidopsis thaliana OX=3702 GN=ASNAP1... [more]
Q54NP61.9e-6044.68Alpha-soluble NSF attachment protein OS=Dictyostelium discoideum OX=44689 GN=snp... [more]
Match NameE-valueIdentityDescription
A0A6J1GYT05.8e-15396.92alpha-soluble NSF attachment protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JUA61.3e-15296.58alpha-soluble NSF attachment protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1CZQ26.4e-15295.55alpha-soluble NSF attachment protein 2-like OS=Momordica charantia OX=3673 GN=LO... [more]
A0A7N2R9Y35.4e-13584.93Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
A0A2P5E0M82.1e-13484.25NSF attachment protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_015780 PE=... [more]
Match NameE-valueIdentityDescription
AT3G56190.13.8e-12575.68alpha-soluble NSF attachment protein 2 [more]
AT3G56190.22.3e-9372.73alpha-soluble NSF attachment protein 2 [more]
AT3G56450.14.6e-6253.52alpha-soluble NSF attachment protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 151..178
NoneNo IPR availablePFAMPF14938SNAPcoord: 7..281
e-value: 2.6E-107
score: 358.5
NoneNo IPR availablePANTHERPTHR13768:SF37ALPHA-SOLUBLE NSF ATTACHMENT PROTEINcoord: 1..292
IPR000744NSF attachment proteinPRINTSPR00448NSFATTACHMNTcoord: 119..136
score: 29.37
coord: 152..175
score: 71.43
coord: 183..202
score: 36.43
coord: 28..47
score: 39.29
coord: 91..108
score: 34.92
coord: 258..278
score: 53.06
IPR000744NSF attachment proteinPANTHERPTHR13768SOLUBLE NSF ATTACHMENT PROTEIN SNAPcoord: 1..292
IPR000744NSF attachment proteinCDDcd15832SNAPcoord: 7..281
e-value: 2.47927E-122
score: 349.185
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 53..86
e-value: 230.0
score: 3.0
coord: 152..185
e-value: 410.0
score: 0.8
coord: 112..145
e-value: 0.0042
score: 26.3
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 112..145
score: 9.6174
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 6..291
e-value: 2.7E-105
score: 353.7
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 7..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG07G004770.2ClCG07G004770.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
biological_process GO:0035494 SNARE complex disassembly
cellular_component GO:0031201 SNARE complex
molecular_function GO:0005483 soluble NSF attachment protein activity
molecular_function GO:0019905 syntaxin binding
molecular_function GO:0005515 protein binding