ClCG07G003260 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG07G003260
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description(1->3)-beta-glucan endohydrolase
LocationCG_Chr07: 3394206 .. 3397435 (+)
RNA-Seq ExpressionClCG07G003260
SyntenyClCG07G003260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGGTCCTCTCTTCCCTCCCCCCACCCATTTCTCCACCGCCGTTCGACCATGTCCGCCGCCGCATTCCGCCGTCTCTCCGCGGCGCTTTTCCTGCTTCTCCAAATCTCCACCGCCGTCGTCGCCATCGGCGTCAACTACGGCACTCTCGCAAACAACCTCCGTCCGCCGGCGGAGGTGGTGAATTTCATCAAAACAAAAACCAAAATCGACCGCGTGAAGATCTTCGATACAAATCCGGATATCCTAAGGGCCTTCGCTGGCTCCGGAATCCTGGTGACGGTGACGGTCGGCAACGGCGAGATCCCCGCTCTGGGGAATCTGGCCACCGCTCGGCAGTGGGTAGCCACCCATATCGCGCCGTTTCACCCTCAGACGCGGTTTAAATACATTGCTGTGGGGAATGAAATCATCCACACGAACGATAAGCCTCTGATCGCTCGTCTGGTTCCCGCCATGAGATCGCTCCACCAAGCTCTGCTTCTCGCCGGAATCAAGGACATTAAGGTAATTTTCTTAATTTGTTGCTCCTCATTTTCTTTTTTCGCCGTTCATGAAAAAGCCGTTTTAAAGCTGTTGGGTTGTAATGATTTATTCTTTTGAGTGATTTAATTTAAATTAGTGATAAAGTTTTTAAAAAAACAATTACATTTATTTCAAAGATTTGTAATTCGTATTATAAAACAGCAATTAATCATTTCCAAATAAATAAATATTATATTATTATATCGAAATTAGAAGTTTGATTCATAATTATTTATATCGTGATATTAACTTTGTACTATATTATGTTTGAATTTGATTTGATTTTGGTAAAAGTTATAAAACAATACAAAATGGTTAATTAGTTTTTATAAAAATTTTACATAGGTTAAAAATAAATTTGGTCATTTCTTTTATTAATACATTAACACCAAAAGTAGTAGAGAATTTTTTTTAAAAAAAAAAAAACATCGATACTAGTGAAGTTTCTGAAATCTTTCAAAAACAAAGGAAAAAAGAAATGTTCTAAAAAACAAAGGAAAAAAGAATGATTAAATTTTATTTTTGTCCTTTAATTTTTAACTTTATTAAATTTTGATCCCACGTAATTTAATATCATATGAGTTTTCACATTTAAATATTATATAGATAGGGTCTTGTGAATTATCTCGTAAGATTAATCGAAGTACAGATAAAAAAAAAAAAAAAAAAAACTTGCAAAATATTTATCTAATCATCGAAGTTTCAAAAGCTTTTTCATTTGGTCATGAAATTCAAGTTTGTATTATTTGTAATTGTTTCTATTATTTTAATTTAATCAAATTAATGATGCAATTTATATGCATTAATATGTTAAATAACACCACGGCCAAAAGTCAAATTAAGTTAGAGACAAACATAAAGATTAGTTCACATCCCAAAAAAAAAAAATTTAAGAGTTTATAAATTAAAATAGAGCGCCATAATGAGCATAGCTACATACGAGTATGTTAATGATCAAGATGGGTGCAGTTCATGAAATACCTAAATTTTTCTCATTTTCTAACAACTTGATTTTCGTTTTCAAGGTGACATCCCCACACTCTTTGGGCATCCTGTCAATCTCGGAGCCACCAAGCATGGGACGGTTTAGGCGAGGGTACGATCGAGCCATCTTTGGTCCCATGCTCCAATTCCTACGAGAAACCAAAGCCCCATTGATGGTGAACCCATATCCTTACTTTGGCTACTCACAAAAAATGGCTAATTACGTCTTATTCAAACCAAATCGTGGGATTTACGACAAGTTCACCAAAATCACCTACACCAACATGTTTGATGGTATGATGGACGCCCTCCATTCCGCCATGAAGAGACTCGGCTACGCCGACATCGATCTCGTCATCGGCGAGACTGGTTGGCCCACCAATTGCGACTACATTGCTTGTAGCCCCGACAATGCTGCTACTTATAATAGGAATTTGATTTGGCATGTCAATTCTGGAAAGGGAACACCGTTGATGCCCAATCGAAAATTTGAGACCTATTTGTTTGGTTTGTTTAATGAGAATCTTAAACCTGGTCCGACCGCTGAGCGGAATTGGGGTTTGTTTCAGCCTGATTTTACTCCAGTCTATGATCTTCCCAGTATCATGCTCGGTGGACGGGTTAGTAATTTTCATTACCCTAAATCTTCTATTTTATTTTAAATCCTTTTGTTTTACTTTTAATTTTTTTAATTAAATTTTTTAAAGGTATCTTTTAATAATTATTTGCTTTTTTGCTTATTAGTTTTTTAATATCTTTCATTGTTTCCTCCAATAATTTTTTAGAATGTTTTCAAAATTTAATGAAGGTTTAAAAAACTAATGCTTTATTTGATAATCATTTGATATTTTATGTCTCGTTTAGTAACTATATAATTTTTTAAAATTGAGCCTATAAATATCTATTTTTACTTACTTATTTATCTACTTTCTATATTTTGAAAAATCAAACTAAATTTTTAAAACTAAAACATAAAAGTAATTTTTAAAAATTTGTTTTTTATTTAAAATTTAGTAAAAAAATTAACTTGTTTACTTAATAAAAATATACATCATTGTAATAAATGTAAGATTATTTTCAAATATAATAAAACTACAATATTTTTAAATATAATAAAATATCATTATCATCGTGTTACATTTTACTATATTTAAAAATATTTTGAGTATTTAAAAATATTTAGAATAATTTTGTTATTTTAAAACTAAATGTAGCTAAAAGCCAATATTAATAACCTCTTTATTTCTTGCAACTTTCCAAACGAACCAAAAATAAATAAATAATTACATTAGATTTGAAATAATTTCTTATAATTTAAGAACCAAGTAAAATTTCCGTTTCATGTTCTCCTACATCAATCCACTAAATCATGTGAGATTTTAAATTACAGCATGGCGGTGGGGGAGGTGGCCGGACGACGCCGGTGGCGCCGTCGGGGAAGAAATGGTGTGTTCCGAAATCCGACGCGGCCATTGAAGCCTTACAGAAGAACATTGACTACGTTTGCAGCTCAAACATCGACTGCCGTCCGATCCAAGCCGGCGGCGCGTGTTTTCTACCCAACGATGTCCGATCCCACGCCGCTTACGTCATGAATTCCTATTACCAGACCTCCGGCCGCCACGATTTCAACTGCGATTTCGGTCACACCGGCGTTCTAACCACCGTCGATCCAAGTAATTACTCATCTTTTTTCTTTCCTCCGATTAAATTGCTGAATTGA

mRNA sequence

GTGGTCCTCTCTTCCCTCCCCCCACCCATTTCTCCACCGCCGTTCGACCATGTCCGCCGCCGCATTCCGCCGTCTCTCCGCGGCGCTTTTCCTGCTTCTCCAAATCTCCACCGCCGTCGTCGCCATCGGCGTCAACTACGGCACTCTCGCAAACAACCTCCGTCCGCCGGCGGAGGTGGTGAATTTCATCAAAACAAAAACCAAAATCGACCGCGTGAAGATCTTCGATACAAATCCGGATATCCTAAGGGCCTTCGCTGGCTCCGGAATCCTGGTGACGGTGACGGTCGGCAACGGCGAGATCCCCGCTCTGGGGAATCTGGCCACCGCTCGGCAGTGGGTAGCCACCCATATCGCGCCGTTTCACCCTCAGACGCGGTTTAAATACATTGCTGTGGGGAATGAAATCATCCACACGAACGATAAGCCTCTGATCGCTCGTCTGGTTCCCGCCATGAGATCGCTCCACCAAGCTCTGCTTCTCGCCGGAATCAAGGACATTAAGGTGACATCCCCACACTCTTTGGGCATCCTGTCAATCTCGGAGCCACCAAGCATGGGACGGTTTAGGCGAGGGTACGATCGAGCCATCTTTGGTCCCATGCTCCAATTCCTACGAGAAACCAAAGCCCCATTGATGGTGAACCCATATCCTTACTTTGGCTACTCACAAAAAATGGCTAATTACGTCTTATTCAAACCAAATCGTGGGATTTACGACAAGTTCACCAAAATCACCTACACCAACATGTTTGATGGTATGATGGACGCCCTCCATTCCGCCATGAAGAGACTCGGCTACGCCGACATCGATCTCGTCATCGGCGAGACTGGTTGGCCCACCAATTGCGACTACATTGCTTGTAGCCCCGACAATGCTGCTACTTATAATAGGAATTTGATTTGGCATGTCAATTCTGGAAAGGGAACACCGTTGATGCCCAATCGAAAATTTGAGACCTATTTGTTTGGTTTGTTTAATGAGAATCTTAAACCTGGTCCGACCGCTGAGCGGAATTGGGGTTTGTTTCAGCCTGATTTTACTCCAGTCTATGATCTTCCCAGTATCATGCTCGGTGGACGGCATGGCGGTGGGGGAGGTGGCCGGACGACGCCGGTGGCGCCGTCGGGGAAGAAATGGTGTGTTCCGAAATCCGACGCGGCCATTGAAGCCTTACAGAAGAACATTGACTACGTTTGCAGCTCAAACATCGACTGCCGTCCGATCCAAGCCGGCGGCGCGTGTTTTCTACCCAACGATGTCCGATCCCACGCCGCTTACGTCATGAATTCCTATTACCAGACCTCCGGCCGCCACGATTTCAACTGCGATTTCGGTCACACCGGCGTTCTAACCACCGTCGATCCAAGTAATTACTCATCTTTTTTCTTTCCTCCGATTAAATTGCTGAATTGA

Coding sequence (CDS)

ATGTCCGCCGCCGCATTCCGCCGTCTCTCCGCGGCGCTTTTCCTGCTTCTCCAAATCTCCACCGCCGTCGTCGCCATCGGCGTCAACTACGGCACTCTCGCAAACAACCTCCGTCCGCCGGCGGAGGTGGTGAATTTCATCAAAACAAAAACCAAAATCGACCGCGTGAAGATCTTCGATACAAATCCGGATATCCTAAGGGCCTTCGCTGGCTCCGGAATCCTGGTGACGGTGACGGTCGGCAACGGCGAGATCCCCGCTCTGGGGAATCTGGCCACCGCTCGGCAGTGGGTAGCCACCCATATCGCGCCGTTTCACCCTCAGACGCGGTTTAAATACATTGCTGTGGGGAATGAAATCATCCACACGAACGATAAGCCTCTGATCGCTCGTCTGGTTCCCGCCATGAGATCGCTCCACCAAGCTCTGCTTCTCGCCGGAATCAAGGACATTAAGGTGACATCCCCACACTCTTTGGGCATCCTGTCAATCTCGGAGCCACCAAGCATGGGACGGTTTAGGCGAGGGTACGATCGAGCCATCTTTGGTCCCATGCTCCAATTCCTACGAGAAACCAAAGCCCCATTGATGGTGAACCCATATCCTTACTTTGGCTACTCACAAAAAATGGCTAATTACGTCTTATTCAAACCAAATCGTGGGATTTACGACAAGTTCACCAAAATCACCTACACCAACATGTTTGATGGTATGATGGACGCCCTCCATTCCGCCATGAAGAGACTCGGCTACGCCGACATCGATCTCGTCATCGGCGAGACTGGTTGGCCCACCAATTGCGACTACATTGCTTGTAGCCCCGACAATGCTGCTACTTATAATAGGAATTTGATTTGGCATGTCAATTCTGGAAAGGGAACACCGTTGATGCCCAATCGAAAATTTGAGACCTATTTGTTTGGTTTGTTTAATGAGAATCTTAAACCTGGTCCGACCGCTGAGCGGAATTGGGGTTTGTTTCAGCCTGATTTTACTCCAGTCTATGATCTTCCCAGTATCATGCTCGGTGGACGGCATGGCGGTGGGGGAGGTGGCCGGACGACGCCGGTGGCGCCGTCGGGGAAGAAATGGTGTGTTCCGAAATCCGACGCGGCCATTGAAGCCTTACAGAAGAACATTGACTACGTTTGCAGCTCAAACATCGACTGCCGTCCGATCCAAGCCGGCGGCGCGTGTTTTCTACCCAACGATGTCCGATCCCACGCCGCTTACGTCATGAATTCCTATTACCAGACCTCCGGCCGCCACGATTTCAACTGCGATTTCGGTCACACCGGCGTTCTAACCACCGTCGATCCAAGTAATTACTCATCTTTTTTCTTTCCTCCGATTAAATTGCTGAATTGA

Protein sequence

MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAFAGSGILVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNEIIHTNDKPLIARLVPAMRSLHQALLLAGIKDIKVTSPHSLGILSISEPPSMGRFRRGYDRAIFGPMLQFLRETKAPLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMMDALHSAMKRLGYADIDLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPVAPSGKKWCVPKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTSGRHDFNCDFGHTGVLTTVDPSNYSSFFFPPIKLLN
Homology
BLAST of ClCG07G003260 vs. NCBI nr
Match: XP_038891662.1 (glucan endo-1,3-beta-glucosidase, partial [Benincasa hispida])

HSP 1 Score: 850.5 bits (2196), Expect = 6.6e-243
Identity = 407/445 (91.46%), Postives = 427/445 (95.96%), Query Frame = 0

Query: 2   SAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDT 61
           SAAAF R+SAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNF+KTKTKIDRVKIFDT
Sbjct: 57  SAAAFHRISAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFLKTKTKIDRVKIFDT 116

Query: 62  NPDILRAFAGSGILVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNEII 121
           NPDILRAFAGSGILVTVTVGNGEIPALG+LA ARQWVA HIAPFHP+TR  YIAVGNEII
Sbjct: 117 NPDILRAFAGSGILVTVTVGNGEIPALGDLAVARQWVAAHIAPFHPRTRIHYIAVGNEII 176

Query: 122 HTNDKPLIARLVPAMRSLHQALLLAGIKDIKVTSPHSLGILSISEPPSMGRFRRGYDRAI 181
           H+NDKPLIARLVPAMRSLH+AL+LAGI+D+KVTSPHSLGILSISEPPSMGRFRRGYDR I
Sbjct: 177 HSNDKPLIARLVPAMRSLHRALVLAGIRDVKVTSPHSLGILSISEPPSMGRFRRGYDRTI 236

Query: 182 FGPMLQFLRETKAPLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMMDA 241
           FGPMLQFLRETKAP MVNPYPYFGYS KMANY LF+PNRGIYDK+TKITYTNMFDGMMDA
Sbjct: 237 FGPMLQFLRETKAPFMVNPYPYFGYSPKMANYALFRPNRGIYDKYTKITYTNMFDGMMDA 296

Query: 242 LHSAMKRLGYADIDLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNRK 301
           L+SAMK+LGYAD+DLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNRK
Sbjct: 297 LYSAMKKLGYADVDLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNRK 356

Query: 302 FETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGR----TTPV 361
           FETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLP+IMLG R GG GGGR     TPV
Sbjct: 357 FETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPNIMLGRRRGGRGGGRRTPSPTPV 416

Query: 362 APSGKKWCVPKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYY 421
           APSG+KWCVP+SDA IEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYY
Sbjct: 417 APSGQKWCVPRSDAPIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYY 476

Query: 422 QTSGRHDFNCDFGHTGVLTTVDPSN 443
           QT+GRHDFNCDFGHTGVLTTV+PS+
Sbjct: 477 QTAGRHDFNCDFGHTGVLTTVNPSH 501

BLAST of ClCG07G003260 vs. NCBI nr
Match: XP_004147695.2 (glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KGN50054.2 hypothetical protein Csa_021427 [Cucumis sativus])

HSP 1 Score: 827.4 bits (2136), Expect = 6.0e-236
Identity = 396/443 (89.39%), Postives = 421/443 (95.03%), Query Frame = 0

Query: 1   MSAAAFRRLSAALFLLLQIS-TAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIF 60
           MSAAA RRLSAALFLLLQIS TAV AIGVNYGTL NNLRPPAEV NF+KTKTKIDRVKIF
Sbjct: 1   MSAAALRRLSAALFLLLQISTTAVFAIGVNYGTLGNNLRPPAEVANFLKTKTKIDRVKIF 60

Query: 61  DTNPDILRAFAGSGILVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNE 120
           DTNPDILRAFAGSGILVTVTVGNG IP L +LA AR+WVA HIAPFHPQTR  YIAVGNE
Sbjct: 61  DTNPDILRAFAGSGILVTVTVGNGLIPNLADLAFAREWVAAHIAPFHPQTRIHYIAVGNE 120

Query: 121 IIHTNDKPLIARLVPAMRSLHQALLLAGIKDIKVTSPHSLGILSISEPPSMGRFRRGYDR 180
           IIH+NDKPLIARLVPAMRSLH+AL+LAGI+D+KVTSPHSLGILSISEPPSMGRFRRGYDR
Sbjct: 121 IIHSNDKPLIARLVPAMRSLHRALVLAGIRDVKVTSPHSLGILSISEPPSMGRFRRGYDR 180

Query: 181 AIFGPMLQFLRETKAPLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMM 240
           AIFGPMLQFLRETKAP MVNPYPYFGYS KMANYVLFKPNRGIYDK+TKITYTNMFD MM
Sbjct: 181 AIFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTKITYTNMFDSMM 240

Query: 241 DALHSAMKRLGYADIDLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPN 300
           DA++SAMK+LGYAD+++V+GETGWPTNCDYIACSPDNAATYNRNL+WHVNSGKGTPLMPN
Sbjct: 241 DAIYSAMKKLGYADVNIVLGETGWPTNCDYIACSPDNAATYNRNLVWHVNSGKGTPLMPN 300

Query: 301 RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPVAP 360
           RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVY LP+IMLGGR  G GG R TPVAP
Sbjct: 301 RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYSLPNIMLGGR--GRGGRRPTPVAP 360

Query: 361 SGKKWCVPKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQT 420
           SGKKWCVP+SDAA++ALQKNIDYVCSSN+DCRPIQAGGACFLPNDVRSHAAYVMNSYYQT
Sbjct: 361 SGKKWCVPRSDAAVDALQKNIDYVCSSNVDCRPIQAGGACFLPNDVRSHAAYVMNSYYQT 420

Query: 421 SGRHDFNCDFGHTGVLTTVDPSN 443
           SGRHD+NCDF HTGVLTTV+PS+
Sbjct: 421 SGRHDYNCDFSHTGVLTTVNPSH 441

BLAST of ClCG07G003260 vs. NCBI nr
Match: XP_008461659.1 (PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis melo] >KAA0050300.1 glucan endo-1,3-beta-glucosidase [Cucumis melo var. makuwa] >TYK03519.1 glucan endo-1,3-beta-glucosidase [Cucumis melo var. makuwa])

HSP 1 Score: 818.1 bits (2112), Expect = 3.6e-233
Identity = 391/442 (88.46%), Postives = 414/442 (93.67%), Query Frame = 0

Query: 1   MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFD 60
           MSAA   RLSAA+FLLLQIST V AIGVNYGTL NNLRPPAEVVNF+KTKTKIDRVKIFD
Sbjct: 1   MSAATLPRLSAAIFLLLQISTTVFAIGVNYGTLGNNLRPPAEVVNFLKTKTKIDRVKIFD 60

Query: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNEI 120
           TNPDILRAFAGSGILVTVTVGNGEIPALGNLA AR WVA HIAPFHPQTR  YIAVGNEI
Sbjct: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLAFARAWVAEHIAPFHPQTRIHYIAVGNEI 120

Query: 121 IHTNDKPLIARLVPAMRSLHQALLLAGIKDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180
           IH+NDKPLIARLVPAMRSLH+AL+LAGI DIKVTSPHSLGILSISEPPSMGRFRRGYDRA
Sbjct: 121 IHSNDKPLIARLVPAMRSLHRALVLAGIWDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180

Query: 181 IFGPMLQFLRETKAPLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMMD 240
           IFGPMLQFLRETKAP MVNPYPYFGYS KMANYVLFKPNRGIYDK+T+ITYTNMFD MMD
Sbjct: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTRITYTNMFDSMMD 240

Query: 241 ALHSAMKRLGYADIDLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNR 300
           A++SAMK+LGYADI +VIGETGWPTNCDYIACSPDNAAT+NRNL+WHVNSGKGTPLMPNR
Sbjct: 241 AIYSAMKKLGYADIKIVIGETGWPTNCDYIACSPDNAATFNRNLVWHVNSGKGTPLMPNR 300

Query: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPVAPS 360
           KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVY+LP+IMLGGR   G     TP+APS
Sbjct: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYNLPNIMLGGRGRRGRRPSPTPIAPS 360

Query: 361 GKKWCVPKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTS 420
            KKWCVP+S AA+EALQKNIDYVCSS +DCRPIQAGGACFLPNDVRSHAAYVMN+YYQTS
Sbjct: 361 AKKWCVPRSGAAVEALQKNIDYVCSSKVDCRPIQAGGACFLPNDVRSHAAYVMNAYYQTS 420

Query: 421 GRHDFNCDFGHTGVLTTVDPSN 443
           GRHD+NCDFG TGVLTTV+PS+
Sbjct: 421 GRHDYNCDFGQTGVLTTVNPSH 442

BLAST of ClCG07G003260 vs. NCBI nr
Match: XP_022957586.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita moschata])

HSP 1 Score: 813.1 bits (2099), Expect = 1.2e-231
Identity = 383/442 (86.65%), Postives = 411/442 (92.99%), Query Frame = 0

Query: 1   MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFD 60
           MSAAAF RL AAL +LLQISTA VAIGVNYGTL NNLRPPAEVVNFIKTKTKIDRVKIFD
Sbjct: 1   MSAAAFHRLFAALLILLQISTAAVAIGVNYGTLGNNLRPPAEVVNFIKTKTKIDRVKIFD 60

Query: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNEI 120
           TNPDILRAFAGSGILVTVTVGNGEIP L +LATARQWVA HI PFHPQTR  YIAVGNEI
Sbjct: 61  TNPDILRAFAGSGILVTVTVGNGEIPGLTDLATARQWVAAHIRPFHPQTRIHYIAVGNEI 120

Query: 121 IHTNDKPLIARLVPAMRSLHQALLLAGIKDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180
           IHTNDKPLIARLVPAM+SLH+AL+LAGI DIKVTSPHSL ILSISEPPSMGRFRRGYDRA
Sbjct: 121 IHTNDKPLIARLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSMGRFRRGYDRA 180

Query: 181 IFGPMLQFLRETKAPLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMMD 240
           IF PMLQFLRETKAP MVNPYPYFGYS  MANY LF PNRG+YDK+TKITYTNMFD MMD
Sbjct: 181 IFAPMLQFLRETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMD 240

Query: 241 ALHSAMKRLGYADIDLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNR 300
           AL+SAMK +GY+D+DLVIGETGWPTNCDY+ACSP+NAAT++RNL+WHVNSGKGTPLMPNR
Sbjct: 241 ALYSAMKNIGYSDVDLVIGETGWPTNCDYVACSPNNAATFHRNLVWHVNSGKGTPLMPNR 300

Query: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPVAPS 360
           KFETYLF LFNENLKPGPTAERNWGLF PDFTPVYD+P+IM+GGRH GG GG   PVAPS
Sbjct: 301 KFETYLFALFNENLKPGPTAERNWGLFYPDFTPVYDVPNIMIGGRHRGGRGGGPKPVAPS 360

Query: 361 GKKWCVPKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTS 420
           GKKWCVPKSDA+++ALQ NIDYVCSSNIDCRPIQAGGACFLPND+RSHA+Y MNSYYQTS
Sbjct: 361 GKKWCVPKSDASVQALQNNIDYVCSSNIDCRPIQAGGACFLPNDIRSHASYAMNSYYQTS 420

Query: 421 GRHDFNCDFGHTGVLTTVDPSN 443
           GR+DFNCDF HTGV+TTV+PS+
Sbjct: 421 GRNDFNCDFSHTGVITTVNPSH 442

BLAST of ClCG07G003260 vs. NCBI nr
Match: KAG6601002.1 (Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 812.8 bits (2098), Expect = 1.5e-231
Identity = 382/442 (86.43%), Postives = 412/442 (93.21%), Query Frame = 0

Query: 1   MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFD 60
           MSAAAF RL AAL +LLQISTA VAIGVNYGTL NNLRPPAEVVNFIKTKTKIDRVKIFD
Sbjct: 1   MSAAAFHRLFAALLILLQISTAAVAIGVNYGTLGNNLRPPAEVVNFIKTKTKIDRVKIFD 60

Query: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNEI 120
           TNPDILRAFAG+GILVTVTVGNGEIP L +LATARQWVA HI PFHPQTR  YIAVGNEI
Sbjct: 61  TNPDILRAFAGTGILVTVTVGNGEIPGLTDLATARQWVAAHIRPFHPQTRIHYIAVGNEI 120

Query: 121 IHTNDKPLIARLVPAMRSLHQALLLAGIKDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180
           IHTNDKPLIARLVPAM+SLH+AL+LAGI DIKVTSPHSL ILSISEPPS+GRFRRGYDRA
Sbjct: 121 IHTNDKPLIARLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSLGRFRRGYDRA 180

Query: 181 IFGPMLQFLRETKAPLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMMD 240
           IF PMLQFLRETKAP MVNPYPYFGYS  MANY LF PNRG+YDK+TKITYTNMFD MMD
Sbjct: 181 IFAPMLQFLRETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMD 240

Query: 241 ALHSAMKRLGYADIDLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNR 300
           AL+SAMK +GY+D+DLVIGETGWPTNCDY+ACSP+NAAT++RNL+WHVNSGKGTPLMPNR
Sbjct: 241 ALYSAMKNIGYSDVDLVIGETGWPTNCDYVACSPNNAATFHRNLVWHVNSGKGTPLMPNR 300

Query: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPVAPS 360
           KFETYLF LFNENLKPGPTAERNWGLF PDFTPVYD+P+IM+GGRH GG GG   PVAPS
Sbjct: 301 KFETYLFALFNENLKPGPTAERNWGLFYPDFTPVYDVPNIMIGGRHRGGRGGGPKPVAPS 360

Query: 361 GKKWCVPKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTS 420
           GKKWCVPKSDA+++ALQKNIDYVCSSNIDCRPIQAGGACFLPND+RSHA+Y MNSYYQTS
Sbjct: 361 GKKWCVPKSDASVQALQKNIDYVCSSNIDCRPIQAGGACFLPNDIRSHASYAMNSYYQTS 420

Query: 421 GRHDFNCDFGHTGVLTTVDPSN 443
           GR+DFNCDF HTGV+TTV+PS+
Sbjct: 421 GRNDFNCDFSHTGVITTVNPSH 442

BLAST of ClCG07G003260 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 3.3e-120
Identity = 219/435 (50.34%), Postives = 283/435 (65.06%), Query Frame = 0

Query: 20  STAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAFAGSGILVTVT 79
           +T  + IGVNYG  A+NL  P  V  F+ TKT IDRVK+FD NP  + AFAG+ I + V+
Sbjct: 20  ATPSLHIGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANPTFISAFAGTPISLAVS 79

Query: 80  VGNGEIPALGNLAT----ARQWVATHIAPFHPQTRFKYIAVGNEIIHTNDKPLIARLVPA 139
           + N  +PAL + AT    AR W+  +++P+ P T    +  GNEI+ + D  LI  L+PA
Sbjct: 80  LPNSALPALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNEILLSTDTNLILSLLPA 139

Query: 140 MRSLHQALLLAGIKDIKVTSPHSLGILSISEP-PSMGRFRRGYDRAIFGPMLQFLRETKA 199
           MR L QAL   G+  ++VT+PH LGIL+ S+  PS   FR GY+  +F  MLQF R+T +
Sbjct: 140 MRRLAQALKAEGLTGVRVTTPHYLGILAPSDGIPSNASFRAGYNTKLFPAMLQFHRDTGS 199

Query: 200 PLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMMDALHSAMKRLGYADI 259
           P MVNPYPYF Y  +  NY LF+PN GIYD  TK+ YT+M D  MDA+++AMK+LGY D+
Sbjct: 200 PFMVNPYPYFSYRPETLNYALFRPNSGIYDPATKLNYTSMLDAQMDAIYTAMKKLGYGDV 259

Query: 260 DLVIGETGWPTNCD--YIACSPDNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFGLFNE 319
           D+ +GE GWPT  +   I      A  +N  +I   +SGKGTPLMPNR FETYLF LF+E
Sbjct: 260 DIAVGEAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTPLMPNRTFETYLFSLFDE 319

Query: 320 NLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTP-----VAPS-GKKWCV 379
           N KPGP AER++GLF PDFTPVYDL  +  G           +P      APS G KWCV
Sbjct: 320 NQKPGPIAERHFGLFNPDFTPVYDLGLLRDGASVAPTPSPNPSPNPSPKPAPSGGGKWCV 379

Query: 380 PKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTSGRHDFN 439
            K  A    LQ NI+Y C   +DC+PIQ+GGACF PN +++HA+YVMN+YYQ +G  D  
Sbjct: 380 AKDGANGTDLQNNINYACGF-VDCKPIQSGGACFSPNSLQAHASYVMNAYYQANGHTDLA 439

Query: 440 CDFGHTGVLTTVDPS 442
           CDF  TG++T+ DPS
Sbjct: 440 CDFKGTGIVTSSDPS 453

BLAST of ClCG07G003260 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 363.2 bits (931), Expect = 4.2e-99
Identity = 191/459 (41.61%), Postives = 285/459 (62.09%), Query Frame = 0

Query: 9   LSAALFLLL------QISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDTN 68
           LS +++ LL        S A   IGVNYG +A+NL PP+E V  +++ T I +V+++  +
Sbjct: 3   LSISIYFLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQS-TSIQKVRLYGAD 62

Query: 69  PDILRAFAGSGILVTVTVGNGEIPAL-GNLATARQWVATHIAPFHPQTRFKYIAVGNEII 128
           P I++A AG+G+ + +   NG++P+L  +   A QW+ +++ PF+P ++   I VGNEI+
Sbjct: 63  PAIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEIL 122

Query: 129 HTNDKPLIARLVPAMRSLHQALLLAGI-KDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 188
            +ND  L+ +L+PAM+++ +AL    +   IKV++ +S+ +L  S+PPS G F  GY   
Sbjct: 123 MSNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG 182

Query: 189 IFGPMLQFLRETKAPLMVNPYPYFGYSQ----KMANYVLFKPNRGIYDKFTKITYTNMFD 248
           + G +LQFL +T +P  +NPYP+F Y      +   + LF+PN G  D  T I YTNMFD
Sbjct: 183 LKG-ILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFD 242

Query: 249 GMMDALHSAMKRLGYADIDLVIGETGWPTNCD--YIACSPDNAATYNRNLIWHVNSGKGT 308
             +DA+HSA+K +G+  +++V+ ETGW +  D   +  S DNA  YN NLI H+ S  GT
Sbjct: 243 AQVDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGT 302

Query: 309 PLMPNRKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRT 368
           PLMP +  +TY+F L++ENLKPGP++ER +GLF+ D + VYD+          G     +
Sbjct: 303 PLMPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDV----------GLAKSSS 362

Query: 369 TPVAPSGK----KWCVPKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAA 428
           +   PSGK     WCVPK  A  E LQ ++D+ C   IDC  IQ GGACF PN+V SHAA
Sbjct: 363 SSQTPSGKVTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAA 422

Query: 429 YVMNSYYQTSGRHDFNCDFGHTGVLTTVDPSNYSSFFFP 450
           Y MN Y+Q S +   +CDF  T  +T+ +PS Y++  +P
Sbjct: 423 YAMNMYFQKSPKQPTDCDFSKTATVTSQNPS-YNNCVYP 448

BLAST of ClCG07G003260 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.6e-93
Identity = 185/448 (41.29%), Postives = 268/448 (59.82%), Query Frame = 0

Query: 13  LFLLLQ---ISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAF 72
           +FLLLQ    + +   +GVNYG L++NL      VN +K+ T I +V++F   P +++AF
Sbjct: 14  VFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKS-TTIQKVRLFGAEPAVIKAF 73

Query: 73  AGSGILVTVTVGNGEIPAL-GNLATARQWVATHIAPFHPQTRFKYIAVGNEIIHTNDKPL 132
           A +G+ + +   NG+IP L  N   A Q+V +++  F+P +    I VGNE++ + D+ L
Sbjct: 74  ANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSGDQKL 133

Query: 133 IARLVPAMRSLHQALLLAGI-KDIKVTSPHSLGILSISEPPSMGRFRRGYDRAIFGPMLQ 192
           I++L+PAM+++  AL  A +   +KV++ H++ +LS S PPS G F  G    +   +LQ
Sbjct: 134 ISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGDTM-KALLQ 193

Query: 193 FLRETKAPLMVNPYPYFGYSQK----MANYVLFKPNRGIYDKFTKITYTNMFDGMMDALH 252
           F     AP M++PYPYF Y  +       + LF+PN G  D      YTNMFD  +DA+H
Sbjct: 194 FQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVDAVH 253

Query: 253 SAMKRLGYADIDLVIGETGWPTNCDYIACSP--DNAATYNRNLIWHVNSGKGTPLMPNRK 312
           SA+  +G+ DI++V+ ETGWP   D     P  DNA  Y  NLI H+ S  GTPLMP + 
Sbjct: 254 SALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMPGKS 313

Query: 313 FETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPVAPSG 372
            +TYLF L++E+ K G ++E+ +GLF+PD +  YD+   +L            TP   + 
Sbjct: 314 IDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVG--LLKNTQNPTTPATPTPTPKAA 373

Query: 373 KKWCVPKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTSG 432
             WCVPK   + + L  NI+Y C   IDC PIQ GGACF PN V++HAAYVMN YYQ++G
Sbjct: 374 GSWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQSAG 433

Query: 433 RHDFNCDFGHTGVLTTVDPSNYSSFFFP 450
           R+ +NCDF  T  LT  +PS Y +  FP
Sbjct: 434 RNSWNCDFSQTATLTNTNPS-YGACNFP 456

BLAST of ClCG07G003260 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.4e-78
Identity = 168/453 (37.09%), Postives = 246/453 (54.30%), Query Frame = 0

Query: 26  IGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAFAGSGILVTVTVGNGEI 85
           IG+ YG  A+NL  P  V   I+    I  V+I+D N D+L+AFA +GI + + V N ++
Sbjct: 27  IGICYGRNADNLPSPNRVSELIQ-HLNIKFVRIYDANIDVLKAFANTGIELMIGVPNADL 86

Query: 86  PALGNL-ATARQWVATHIAPFHPQTRFKYIAVGNEIIHTNDKPLIARLVPAMRSLHQALL 145
            A     +    W++ +I P++P T+   I+VG E+    D      ++PAMR++H AL 
Sbjct: 87  LAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDN-ATGLVLPAMRNIHTALK 146

Query: 146 LAGI-KDIKVTSPHSLGILSISEPPSMGRFRRGYDRAIFGPMLQFLRETKAPLMVNPYPY 205
            +G+ K IK++S HSL ILS S PPS   F + +  A   PML+FL E ++P M++ YPY
Sbjct: 147 KSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHS-AFLKPMLEFLVENESPFMIDLYPY 206

Query: 206 FGY---SQKM-ANYVLFKPNRGIYDKFTKITYTNMFDGMMDALHSAMKRLGYADIDLVIG 265
           + Y   ++K+   Y LF+ +  + D  T + Y+NMFD  +DA++ A+  + +  + +++ 
Sbjct: 207 YAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVT 266

Query: 266 ETGWPT--NCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFGLFNENLKPG 325
           E+GWP+  +    A +P+NA  YN NLI HV    GTP  P  + + YLF LFNEN KPG
Sbjct: 267 ESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPG 326

Query: 326 PTAERNWGLFQPDFTPVYDL------------------------PSIMLGGRH----GGG 385
             +ERNWG+F  + T VY L                         S ++ G      GGG
Sbjct: 327 IESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGG 386

Query: 386 GGGRTTPVAPSGKKWCVPKSDAAIEALQKNIDYVCS-SNIDCRPIQAGGACFLPNDVRSH 442
           GGG T       KKWC+  S A++  LQ  +D+ C   N+DC  +Q    CF P+ V SH
Sbjct: 387 GGGGT-------KKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSH 446

BLAST of ClCG07G003260 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 5.9e-77
Identity = 172/445 (38.65%), Postives = 243/445 (54.61%), Query Frame = 0

Query: 10  SAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAF 69
           S ++ LLL        +GV YG  A++L  P++VV  I+ +  I  V+I+D N  +L+AF
Sbjct: 10  SISILLLLLDCCYGGKVGVCYGRSADDLPTPSKVVQLIQ-QHNIKYVRIYDYNSQVLKAF 69

Query: 70  AGSGILVTVTVGNGEIPALG-NLATARQWVATHIAPFHPQTRFKYIAVGNEIIHTNDKPL 129
             + I + + V N ++ A   + +    W+   + P++P T+  YI VG E   + D P 
Sbjct: 70  GNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAE---STDDPH 129

Query: 130 I---ARLVPAMRSLHQALLLAGI-KDIKVTSPHSLGILSISEPPSMGRFRRGYDRAIFGP 189
           I   + +VPAM+++  AL   G+ + IKV++  SLGILS S PPS G F   Y      P
Sbjct: 130 INASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSY-AYFLRP 189

Query: 190 MLQFLRETKAPLMVNPYPYFGYSQKMAN----YVLFKPNRGIYDKFTKITYTNMFDGMMD 249
           ML+FL E K+P M++ YPY+ Y     N    YVLF+ +  + D  T + Y NMFD  +D
Sbjct: 190 MLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVD 249

Query: 250 ALHSAMKRLGYADIDLVIGETGWPTN---CDYIACSPDNAATYNRNLIWHVNSGKGTPLM 309
           AL+ A+  L +  I +++ ETGWPT     +  A S DNA TYN N+I HV + +GTP  
Sbjct: 250 ALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAK 309

Query: 310 PNRKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPV 369
           P      Y+F LFNEN K G  +ERNWGLF PD T VY L     G  +G       T  
Sbjct: 310 PGEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLD--FTGKSNGFHSNSSGTNS 369

Query: 370 APSGKKWCVPKSDAAIEALQKNIDYVCS-SNIDCRPIQAGGACFLPNDVRSHAAYVMNSY 429
           + S   WC+  S A+   L+  +D+ C   N+DC  IQ    CF P+ + SHA++V NSY
Sbjct: 370 SGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSY 429

Query: 430 YQTSGRHDFNCDFGHTGVLTTVDPS 442
           +Q +   D  C FG  GV    DPS
Sbjct: 430 FQQNRATDVACSFGGAGVKVNKDPS 447

BLAST of ClCG07G003260 vs. ExPASy TrEMBL
Match: A0A0A0KS71 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G181480 PE=3 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 3.8e-236
Identity = 396/442 (89.59%), Postives = 420/442 (95.02%), Query Frame = 0

Query: 1   MSAAAFRRLSAALFLLLQIS-TAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIF 60
           MSAAA RRLSAALFLLLQIS TAV AIGVNYGTL NNLRPPAEV NF+KTKTKIDRVKIF
Sbjct: 1   MSAAALRRLSAALFLLLQISTTAVFAIGVNYGTLGNNLRPPAEVANFLKTKTKIDRVKIF 60

Query: 61  DTNPDILRAFAGSGILVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNE 120
           DTNPDILRAFAGSGILVTVTVGNG IP L +LA AR+WVA HIAPFHPQTR  YIAVGNE
Sbjct: 61  DTNPDILRAFAGSGILVTVTVGNGLIPNLADLAFAREWVAAHIAPFHPQTRIHYIAVGNE 120

Query: 121 IIHTNDKPLIARLVPAMRSLHQALLLAGIKDIKVTSPHSLGILSISEPPSMGRFRRGYDR 180
           IIH+NDKPLIARLVPAMRSLH+AL+LAGI+D+KVTSPHSLGILSISEPPSMGRFRRGYDR
Sbjct: 121 IIHSNDKPLIARLVPAMRSLHRALVLAGIRDVKVTSPHSLGILSISEPPSMGRFRRGYDR 180

Query: 181 AIFGPMLQFLRETKAPLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMM 240
           AIFGPMLQFLRETKAP MVNPYPYFGYS KMANYVLFKPNRGIYDK+TKITYTNMFD MM
Sbjct: 181 AIFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTKITYTNMFDSMM 240

Query: 241 DALHSAMKRLGYADIDLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPN 300
           DA++SAMK+LGYAD+++V+GETGWPTNCDYIACSPDNAATYNRNL+WHVNSGKGTPLMPN
Sbjct: 241 DAIYSAMKKLGYADVNIVLGETGWPTNCDYIACSPDNAATYNRNLVWHVNSGKGTPLMPN 300

Query: 301 RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPVAP 360
           RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVY LP+IMLGGR  G GG R TPVAP
Sbjct: 301 RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYSLPNIMLGGR--GRGGRRPTPVAP 360

Query: 361 SGKKWCVPKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQT 420
           SGKKWCVP+SDAA++ALQKNIDYVCSSN+DCRPIQAGGACFLPNDVRSHAAYVMNSYYQT
Sbjct: 361 SGKKWCVPRSDAAVDALQKNIDYVCSSNVDCRPIQAGGACFLPNDVRSHAAYVMNSYYQT 420

Query: 421 SGRHDFNCDFGHTGVLTTVDPS 442
           SGRHD+NCDF HTGVLTTV+PS
Sbjct: 421 SGRHDYNCDFSHTGVLTTVNPS 440

BLAST of ClCG07G003260 vs. ExPASy TrEMBL
Match: A0A5D3BZM0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00090 PE=3 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 1.8e-233
Identity = 391/442 (88.46%), Postives = 414/442 (93.67%), Query Frame = 0

Query: 1   MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFD 60
           MSAA   RLSAA+FLLLQIST V AIGVNYGTL NNLRPPAEVVNF+KTKTKIDRVKIFD
Sbjct: 1   MSAATLPRLSAAIFLLLQISTTVFAIGVNYGTLGNNLRPPAEVVNFLKTKTKIDRVKIFD 60

Query: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNEI 120
           TNPDILRAFAGSGILVTVTVGNGEIPALGNLA AR WVA HIAPFHPQTR  YIAVGNEI
Sbjct: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLAFARAWVAEHIAPFHPQTRIHYIAVGNEI 120

Query: 121 IHTNDKPLIARLVPAMRSLHQALLLAGIKDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180
           IH+NDKPLIARLVPAMRSLH+AL+LAGI DIKVTSPHSLGILSISEPPSMGRFRRGYDRA
Sbjct: 121 IHSNDKPLIARLVPAMRSLHRALVLAGIWDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180

Query: 181 IFGPMLQFLRETKAPLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMMD 240
           IFGPMLQFLRETKAP MVNPYPYFGYS KMANYVLFKPNRGIYDK+T+ITYTNMFD MMD
Sbjct: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTRITYTNMFDSMMD 240

Query: 241 ALHSAMKRLGYADIDLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNR 300
           A++SAMK+LGYADI +VIGETGWPTNCDYIACSPDNAAT+NRNL+WHVNSGKGTPLMPNR
Sbjct: 241 AIYSAMKKLGYADIKIVIGETGWPTNCDYIACSPDNAATFNRNLVWHVNSGKGTPLMPNR 300

Query: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPVAPS 360
           KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVY+LP+IMLGGR   G     TP+APS
Sbjct: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYNLPNIMLGGRGRRGRRPSPTPIAPS 360

Query: 361 GKKWCVPKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTS 420
            KKWCVP+S AA+EALQKNIDYVCSS +DCRPIQAGGACFLPNDVRSHAAYVMN+YYQTS
Sbjct: 361 AKKWCVPRSGAAVEALQKNIDYVCSSKVDCRPIQAGGACFLPNDVRSHAAYVMNAYYQTS 420

Query: 421 GRHDFNCDFGHTGVLTTVDPSN 443
           GRHD+NCDFG TGVLTTV+PS+
Sbjct: 421 GRHDYNCDFGQTGVLTTVNPSH 442

BLAST of ClCG07G003260 vs. ExPASy TrEMBL
Match: A0A1S3CF05 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103500207 PE=3 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 1.8e-233
Identity = 391/442 (88.46%), Postives = 414/442 (93.67%), Query Frame = 0

Query: 1   MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFD 60
           MSAA   RLSAA+FLLLQIST V AIGVNYGTL NNLRPPAEVVNF+KTKTKIDRVKIFD
Sbjct: 1   MSAATLPRLSAAIFLLLQISTTVFAIGVNYGTLGNNLRPPAEVVNFLKTKTKIDRVKIFD 60

Query: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNEI 120
           TNPDILRAFAGSGILVTVTVGNGEIPALGNLA AR WVA HIAPFHPQTR  YIAVGNEI
Sbjct: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLAFARAWVAEHIAPFHPQTRIHYIAVGNEI 120

Query: 121 IHTNDKPLIARLVPAMRSLHQALLLAGIKDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180
           IH+NDKPLIARLVPAMRSLH+AL+LAGI DIKVTSPHSLGILSISEPPSMGRFRRGYDRA
Sbjct: 121 IHSNDKPLIARLVPAMRSLHRALVLAGIWDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180

Query: 181 IFGPMLQFLRETKAPLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMMD 240
           IFGPMLQFLRETKAP MVNPYPYFGYS KMANYVLFKPNRGIYDK+T+ITYTNMFD MMD
Sbjct: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPKMANYVLFKPNRGIYDKYTRITYTNMFDSMMD 240

Query: 241 ALHSAMKRLGYADIDLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNR 300
           A++SAMK+LGYADI +VIGETGWPTNCDYIACSPDNAAT+NRNL+WHVNSGKGTPLMPNR
Sbjct: 241 AIYSAMKKLGYADIKIVIGETGWPTNCDYIACSPDNAATFNRNLVWHVNSGKGTPLMPNR 300

Query: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPVAPS 360
           KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVY+LP+IMLGGR   G     TP+APS
Sbjct: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYNLPNIMLGGRGRRGRRPSPTPIAPS 360

Query: 361 GKKWCVPKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTS 420
            KKWCVP+S AA+EALQKNIDYVCSS +DCRPIQAGGACFLPNDVRSHAAYVMN+YYQTS
Sbjct: 361 AKKWCVPRSGAAVEALQKNIDYVCSSKVDCRPIQAGGACFLPNDVRSHAAYVMNAYYQTS 420

Query: 421 GRHDFNCDFGHTGVLTTVDPSN 443
           GRHD+NCDFG TGVLTTV+PS+
Sbjct: 421 GRHDYNCDFGQTGVLTTVNPSH 442

BLAST of ClCG07G003260 vs. ExPASy TrEMBL
Match: A0A6J1GZM3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111458933 PE=3 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 5.7e-232
Identity = 383/442 (86.65%), Postives = 411/442 (92.99%), Query Frame = 0

Query: 1   MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFD 60
           MSAAAF RL AAL +LLQISTA VAIGVNYGTL NNLRPPAEVVNFIKTKTKIDRVKIFD
Sbjct: 1   MSAAAFHRLFAALLILLQISTAAVAIGVNYGTLGNNLRPPAEVVNFIKTKTKIDRVKIFD 60

Query: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNEI 120
           TNPDILRAFAGSGILVTVTVGNGEIP L +LATARQWVA HI PFHPQTR  YIAVGNEI
Sbjct: 61  TNPDILRAFAGSGILVTVTVGNGEIPGLTDLATARQWVAAHIRPFHPQTRIHYIAVGNEI 120

Query: 121 IHTNDKPLIARLVPAMRSLHQALLLAGIKDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180
           IHTNDKPLIARLVPAM+SLH+AL+LAGI DIKVTSPHSL ILSISEPPSMGRFRRGYDRA
Sbjct: 121 IHTNDKPLIARLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSMGRFRRGYDRA 180

Query: 181 IFGPMLQFLRETKAPLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMMD 240
           IF PMLQFLRETKAP MVNPYPYFGYS  MANY LF PNRG+YDK+TKITYTNMFD MMD
Sbjct: 181 IFAPMLQFLRETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMD 240

Query: 241 ALHSAMKRLGYADIDLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNR 300
           AL+SAMK +GY+D+DLVIGETGWPTNCDY+ACSP+NAAT++RNL+WHVNSGKGTPLMPNR
Sbjct: 241 ALYSAMKNIGYSDVDLVIGETGWPTNCDYVACSPNNAATFHRNLVWHVNSGKGTPLMPNR 300

Query: 301 KFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPVAPS 360
           KFETYLF LFNENLKPGPTAERNWGLF PDFTPVYD+P+IM+GGRH GG GG   PVAPS
Sbjct: 301 KFETYLFALFNENLKPGPTAERNWGLFYPDFTPVYDVPNIMIGGRHRGGRGGGPKPVAPS 360

Query: 361 GKKWCVPKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTS 420
           GKKWCVPKSDA+++ALQ NIDYVCSSNIDCRPIQAGGACFLPND+RSHA+Y MNSYYQTS
Sbjct: 361 GKKWCVPKSDASVQALQNNIDYVCSSNIDCRPIQAGGACFLPNDIRSHASYAMNSYYQTS 420

Query: 421 GRHDFNCDFGHTGVLTTVDPSN 443
           GR+DFNCDF HTGV+TTV+PS+
Sbjct: 421 GRNDFNCDFSHTGVITTVNPSH 442

BLAST of ClCG07G003260 vs. ExPASy TrEMBL
Match: A0A6J1JYX6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111489139 PE=3 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 3.7e-231
Identity = 384/443 (86.68%), Postives = 414/443 (93.45%), Query Frame = 0

Query: 1   MSAAAFRRLSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFD 60
           MSAAAF RL AAL +LLQISTA VAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFD
Sbjct: 1   MSAAAFHRLFAALLILLQISTAAVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFD 60

Query: 61  TNPDILRAFAGSGILVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNEI 120
           TNPDILRAFAG+GILVTVTVGNGEIPAL +LATARQWVA HI PFHPQTR  YIAVGNEI
Sbjct: 61  TNPDILRAFAGTGILVTVTVGNGEIPALTDLATARQWVAAHIRPFHPQTRINYIAVGNEI 120

Query: 121 IHTNDKPLIARLVPAMRSLHQALLLAGIKDIKVTSPHSLGILSISEPPSMGRFRRGYDRA 180
           IHTNDKPLIARLVPAM+SLH+AL+LAGI DIKVTSPHSL ILSISEPPSMGRFRRGYDRA
Sbjct: 121 IHTNDKPLIARLVPAMKSLHRALVLAGITDIKVTSPHSLAILSISEPPSMGRFRRGYDRA 180

Query: 181 IFGPMLQFLRETKAPLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMMD 240
           IFGPMLQFLRETKAP MVNPYPYFGYS  MANY LF PNRG+YDK+TKITYTNMFD MMD
Sbjct: 181 IFGPMLQFLRETKAPFMVNPYPYFGYSPNMANYALFGPNRGVYDKYTKITYTNMFDAMMD 240

Query: 241 ALHSAMKRLGYADIDLVIGETGWPTNCD-YIACSPDNAATYNRNLIWHVNSGKGTPLMPN 300
           AL+SAMK +GY+D+DLVIGETGWP+NCD Y+ACSP NAAT++RNL+WHVNSGKGTPLMPN
Sbjct: 241 ALYSAMKNIGYSDVDLVIGETGWPSNCDGYVACSPGNAATFHRNLVWHVNSGKGTPLMPN 300

Query: 301 RKFETYLFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPVAP 360
           RKFETYLF LFNENLKPGPTAERNWGLF PDFTPVYD+P+IM+GGRH GG GGR  PVAP
Sbjct: 301 RKFETYLFALFNENLKPGPTAERNWGLFHPDFTPVYDVPNIMIGGRHRGGRGGRPKPVAP 360

Query: 361 SGKKWCVPKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQT 420
           SGKKWCVPKSDA+++ALQKNIDYVCSSNIDCRPIQAGGACFLPND+R+HA+Y MNSYYQT
Sbjct: 361 SGKKWCVPKSDASVQALQKNIDYVCSSNIDCRPIQAGGACFLPNDIRAHASYAMNSYYQT 420

Query: 421 SGRHDFNCDFGHTGVLTTVDPSN 443
           SGR+DFNCDF  TGV+TTV+PS+
Sbjct: 421 SGRNDFNCDFSLTGVITTVNPSH 443

BLAST of ClCG07G003260 vs. TAIR 10
Match: AT3G55430.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 510.0 bits (1312), Expect = 2.0e-144
Identity = 250/439 (56.95%), Postives = 324/439 (73.80%), Query Frame = 0

Query: 9   LSAALFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRA 68
           L AA+F  L I   + AIGVNYGTL  NL PP +V NFIKT+T ID VKIFD NPDILRA
Sbjct: 14  LCAAVF--LTIPAVISAIGVNYGTL-GNLPPPTQVANFIKTQTSIDSVKIFDVNPDILRA 73

Query: 69  FAGSGILVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNEIIHTNDKPL 128
           FAG+GI V VTV NG+IPAL N   AR+WV+ +I PFHPQT+ KYI+VGNEI+ T D  +
Sbjct: 74  FAGTGISVVVTVPNGDIPALANGRQARRWVSVNILPFHPQTKIKYISVGNEILLTGDNNM 133

Query: 129 IARLVPAMRSLHQALLLAGIKDIKVTSPHSLGILS--ISEPPSMGRFRRGYDRAIFGPML 188
           I  L+PAMR+L+ AL+ AG++D+KVT+ HSL I++  ++  PS GRFR G+D+ I  P+L
Sbjct: 134 INNLLPAMRNLNNALVRAGVRDVKVTTAHSLNIIAYDLTGAPSSGRFRPGWDKGILAPIL 193

Query: 189 QFLRETKAPLMVNPYPYFGYSQKMANYVLFK-PNRGIYDKFTKITYTNMFDGMMDALHSA 248
            + R TK+P MVNPYPYFG+  K  N+ +F+ P + + D FT+  YTNMFD +MD+ +SA
Sbjct: 194 AYHRRTKSPFMVNPYPYFGFDPKNVNFAIFRTPYKAVRDPFTRHVYTNMFDALMDSTYSA 253

Query: 249 MKRLGYADIDLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNRKFETY 308
           MK LGY D+++V+GETGWP+ CD   CSP NAA +N N+I     G+GTPLMPNR+FETY
Sbjct: 254 MKALGYGDVNIVVGETGWPSACDAPWCSPANAAWFNLNIIKRA-QGQGTPLMPNRRFETY 313

Query: 309 LFGLFNENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPVAPS--GKK 368
           +FGLFNE  KPGPTAERNWGLF+ DF+PVYD+  +    R+G GGGGR    APS  G K
Sbjct: 314 IFGLFNEEGKPGPTAERNWGLFRADFSPVYDVGLL----RNGQGGGGRPALPAPSTAGGK 373

Query: 369 WCVPKSDAAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTSGRH 428
           WCV +S A    LQ +I++VC   +DC+PIQAGG+CF P+ +R+HA++VMN+Y+Q+ GR 
Sbjct: 374 WCVARSGATNTQLQDSINWVCGQGVDCKPIQAGGSCFNPSSLRTHASFVMNAYFQSHGRT 433

Query: 429 DFNCDFGHTGVLTTVDPSN 443
           D  C+F  TG++   +PSN
Sbjct: 434 DGACNFSGTGMIVGNNPSN 444

BLAST of ClCG07G003260 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 481.9 bits (1239), Expect = 5.8e-136
Identity = 227/431 (52.67%), Postives = 309/431 (71.69%), Query Frame = 0

Query: 15  LLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAFAGSGI 74
           LL+  + +  +IGVNYGTLANNL PP +V  F+   T I+R+++FDT+P IL+AFA +GI
Sbjct: 19  LLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQILQAFANTGI 78

Query: 75  LVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNEIIHTNDKPLIARLVP 134
            VTVTV N +IP L NL++A+QW++ HI P  P T    I VGNE+I T D  LI  L+P
Sbjct: 79  AVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTADHLLIRTLIP 138

Query: 135 AMRSLHQALLLAGI-KDIKVTSPHSLGILSISEPPSMGRFRRGYDRAIFGPMLQFLRETK 194
           AM+SLH AL+ A + + I++++PHSLG L+ S PPS  +FRRGYD  +  P+L FLR T 
Sbjct: 139 AMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLKPLLSFLRSTS 198

Query: 195 APLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMMDALHSAMKRLGYAD 254
           +P +VNPYP+FGYS +  ++ LF+PN G++D+ TK+ YTNM D  +D+++SAM +LG++D
Sbjct: 199 SPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSVYSAMDKLGFSD 258

Query: 255 IDLVIGETGWPT--NCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFGLFN 314
           +++VIGE GWP+  + D I    D AA +N+NLI  V+SG GTPLMPNR FETY+F LFN
Sbjct: 259 VEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNRTFETYIFALFN 318

Query: 315 ENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPV-APSGKKWCVPKSD 374
           ENLK GPT+ERN+G+F+ D TP+YD+  +    R         +PV   S K+WCV K+ 
Sbjct: 319 ENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDPVYNPRSPVRGSSSKRWCVTKAG 378

Query: 375 AAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTSGRHDFNCDFG 434
           A   ALQ+NIDYVC   +DCRPI  GG C+LPN V++H+ Y MN YYQT  +H+F+CDF 
Sbjct: 379 AETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAMNLYYQTMEKHEFDCDFD 438

Query: 435 HTGVLTTVDPS 442
           +TG +TT+DPS
Sbjct: 439 NTGEITTIDPS 449

BLAST of ClCG07G003260 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 481.9 bits (1239), Expect = 5.8e-136
Identity = 227/431 (52.67%), Postives = 309/431 (71.69%), Query Frame = 0

Query: 15  LLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAFAGSGI 74
           LL+  + +  +IGVNYGTLANNL PP +V  F+   T I+R+++FDT+P IL+AFA +GI
Sbjct: 19  LLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQILQAFANTGI 78

Query: 75  LVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNEIIHTNDKPLIARLVP 134
            VTVTV N +IP L NL++A+QW++ HI P  P T    I VGNE+I T D  LI  L+P
Sbjct: 79  AVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTADHLLIRTLIP 138

Query: 135 AMRSLHQALLLAGI-KDIKVTSPHSLGILSISEPPSMGRFRRGYDRAIFGPMLQFLRETK 194
           AM+SLH AL+ A + + I++++PHSLG L+ S PPS  +FRRGYD  +  P+L FLR T 
Sbjct: 139 AMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLKPLLSFLRSTS 198

Query: 195 APLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMMDALHSAMKRLGYAD 254
           +P +VNPYP+FGYS +  ++ LF+PN G++D+ TK+ YTNM D  +D+++SAM +LG++D
Sbjct: 199 SPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSVYSAMDKLGFSD 258

Query: 255 IDLVIGETGWPT--NCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFGLFN 314
           +++VIGE GWP+  + D I    D AA +N+NLI  V+SG GTPLMPNR FETY+F LFN
Sbjct: 259 VEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNRTFETYIFALFN 318

Query: 315 ENLKPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPV-APSGKKWCVPKSD 374
           ENLK GPT+ERN+G+F+ D TP+YD+  +    R         +PV   S K+WCV K+ 
Sbjct: 319 ENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDPVYNPRSPVRGSSSKRWCVTKAG 378

Query: 375 AAIEALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTSGRHDFNCDFG 434
           A   ALQ+NIDYVC   +DCRPI  GG C+LPN V++H+ Y MN YYQT  +H+F+CDF 
Sbjct: 379 AETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAMNLYYQTMEKHEFDCDFD 438

Query: 435 HTGVLTTVDPS 442
           +TG +TT+DPS
Sbjct: 439 NTGEITTIDPS 449

BLAST of ClCG07G003260 vs. TAIR 10
Match: AT2G39640.1 (glycosyl hydrolase family 17 protein )

HSP 1 Score: 443.7 bits (1140), Expect = 1.7e-124
Identity = 218/427 (51.05%), Postives = 286/427 (66.98%), Query Frame = 0

Query: 15  LLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAFAGSGI 74
           ++ Q+S    AIG+NYGTL  NL+PP +VV+FIKTKT  D VKI+D NPDILRAFAGS I
Sbjct: 19  VIFQLSAVTSAIGINYGTL-GNLQPPQQVVDFIKTKTTFDSVKIYDANPDILRAFAGSEI 78

Query: 75  LVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNEIIHTNDKPLIARLVP 134
            +T+ V NG IPA+ N+A ARQWVA ++ PF  Q +FKY+ VGNEI+ +ND  LI+ LVP
Sbjct: 79  NITIMVPNGNIPAMVNVANARQWVAANVLPFQQQIKFKYVCVGNEILASNDNNLISNLVP 138

Query: 135 AMRSLHQALLLAGIKDIKVTSPHSLGILSISEPPSMGRFRRGYDRAIFGPMLQFLRETKA 194
           AM+SL++AL  + +  IKVT+PH+  I      PS  RF     + IF  +L+F R+ K+
Sbjct: 139 AMQSLNEALKASNLTYIKVTTPHAFTISYNRNTPSESRFTND-QKDIFTKILEFHRQAKS 198

Query: 195 PLMVNPYPYFGYSQKMANYVLFKPNRGIYDKFTKITYTNMFDGMMDALHSAMKRLGYADI 254
           P M+N Y +F       NY +F P+  I D  T+ TYTNMFD +MDA +SAMK LGY D+
Sbjct: 199 PFMINAYTFFTMDTNNVNYAIFGPSNAITDTNTQQTYTNMFDAVMDATYSAMKALGYGDV 258

Query: 255 DLVIGETGWPTNCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNRKFETYLFGLFNENL 314
           D+ +GETGWPT CD   CSP NA  YN N+I       GTPLMPNR  + ++F LFNE+ 
Sbjct: 259 DIAVGETGWPTACDASWCSPQNAENYNLNIIKRAQV-IGTPLMPNRHIDIFIFALFNEDG 318

Query: 315 KPGPTAERNWGLFQPDFTPVYDLPSIMLGGRHGGGGGGRTTPVAPSGKKWCVPKSDAAIE 374
           KPGPT ERNWG+F+PDF+P+YD+      G   GGG     P   +  KWCV K +A + 
Sbjct: 319 KPGPTRERNWGIFKPDFSPMYDV------GVLKGGGSPLPFPPINNNGKWCVGKPEATLM 378

Query: 375 ALQKNIDYVCSSNIDCRPIQAGGACFLPNDVRSHAAYVMNSYYQTSGRHDFNCDFGHTGV 434
            LQ NID+VCS  IDC PI  GG CF  N++ + ++++MN+YYQ+ G  D  CDF  TG+
Sbjct: 379 QLQANIDWVCSHGIDCTPISPGGICFDNNNMTTRSSFIMNAYYQSKGCVDVVCDFSGTGI 436

Query: 435 LTTVDPS 442
           +T+ +PS
Sbjct: 439 VTSTNPS 436

BLAST of ClCG07G003260 vs. TAIR 10
Match: AT3G46570.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 380.2 bits (975), Expect = 2.4e-105
Identity = 195/330 (59.09%), Postives = 242/330 (73.33%), Query Frame = 0

Query: 13  LFLLLQISTAVVAIGVNYGTLANNLRPPAEVVNFIKTKTKIDRVKIFDTNPDILRAFAG- 72
           L L+L  S A   +GVNYGT+ANNL PP +VVNFIKTKT I+ VKIFDTN DIL AF+G 
Sbjct: 15  LTLILPFS-AAYQVGVNYGTVANNLPPPHQVVNFIKTKTIINHVKIFDTNHDILAAFSGI 74

Query: 73  SGILVTVTVGNGEIPALGNLATARQWVATHIAPFHPQTRFKYIAVGNEIIHTNDKPLIAR 132
           +GI +TVTV N +I +L  L+ AR W++ ++ PF   T  +YIAVGNE++ T+DK LI  
Sbjct: 75  TGISLTVTVPNSDIISLSKLSNARSWLSDNLLPFLLTTSIRYIAVGNEVVATSDKTLITH 134

Query: 133 LVPAMRSLHQALLLAGIKDIKVTSPHSLGILS-ISEPPSMGRFRRGYDRAIFGPMLQFLR 192
           L+PAM +L  AL LA +  I V++PHSLGILS  SEPPS G+FR+GYD+AIF P+L F  
Sbjct: 135 LLPAMETLTLALHLANVSRILVSTPHSLGILSGSSEPPSSGKFRKGYDKAIFSPILDFHN 194

Query: 193 ETKAPLMVNPYPYFGYSQKMANYVLFKPNRGIY-DKFTKITYTNMFDGMMDALHSAMKRL 252
            TK+P MVNPYPYFG+  +  NY LF  N  +Y D  TK+ YTNMFD  +DA++SAMKR 
Sbjct: 195 RTKSPFMVNPYPYFGFGPETLNYALFNTNDVVYVDPVTKLNYTNMFDAQLDAVYSAMKRF 254

Query: 253 GYADIDLVIGETGWPT--NCDYIACSPDNAATYNRNLIWHVNSGKGTPLMPNRKFETYLF 312
           GY D+D+V+ ETGWP+    +      D AA YN NLI HVNSGKGTPLMPNR FETY+F
Sbjct: 255 GYGDVDIVVAETGWPSAGEPNQTGVGLDYAAAYNGNLIKHVNSGKGTPLMPNRVFETYVF 314

Query: 313 GLFNENLKPGPTAERNWGLFQPDFTPVYDL 338
            LFNENLK    +E+N+GLF+PDFTPVYD+
Sbjct: 315 SLFNENLK-SSVSEQNFGLFKPDFTPVYDV 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038891662.16.6e-24391.46glucan endo-1,3-beta-glucosidase, partial [Benincasa hispida][more]
XP_004147695.26.0e-23689.39glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KGN50054.2 hypothetical prot... [more]
XP_008461659.13.6e-23388.46PREDICTED: glucan endo-1,3-beta-glucosidase [Cucumis melo] >KAA0050300.1 glucan ... [more]
XP_022957586.11.2e-23186.65glucan endo-1,3-beta-glucosidase-like [Cucurbita moschata][more]
KAG6601002.11.5e-23186.43Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
P524093.3e-12050.34Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1[more]
Q9M0694.2e-9941.61Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q94G861.6e-9341.29Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Q8VYE52.4e-7837.09Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9FJU95.9e-7738.65Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Match NameE-valueIdentityDescription
A0A0A0KS713.8e-23689.59(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G181480 PE=3... [more]
A0A5D3BZM01.8e-23388.46(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CF051.8e-23388.46(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103500207 PE=3 SV... [more]
A0A6J1GZM35.7e-23286.65(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111458933 P... [more]
A0A6J1JYX63.7e-23186.68(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111489139 PE=... [more]
Match NameE-valueIdentityDescription
AT3G55430.12.0e-14456.95O-Glycosyl hydrolases family 17 protein [more]
AT5G24318.15.8e-13652.67O-Glycosyl hydrolases family 17 protein [more]
AT5G24318.25.8e-13652.67O-Glycosyl hydrolases family 17 protein [more]
AT2G39640.11.7e-12451.05glycosyl hydrolase family 17 protein [more]
AT3G46570.12.4e-10559.09Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 363..446
e-value: 6.3E-40
score: 148.6
IPR012946X8 domainPFAMPF07983X8coord: 363..433
e-value: 4.3E-20
score: 72.2
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 24..340
e-value: 4.4E-114
score: 382.7
NoneNo IPR availableGENE3D1.20.58.1040coord: 360..446
e-value: 1.6E-10
score: 43.2
NoneNo IPR availablePANTHERPTHR32227:SF371GLUCAN ENDO-1,3-BETA-GLUCOSIDASE-LIKEcoord: 12..443
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 26..337
e-value: 1.0E-83
score: 281.5
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 12..443
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 26..337

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG07G003260.2ClCG07G003260.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds