ClCG06G016480 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG06G016480
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4
LocationCG_Chr06: 29700636 .. 29703798 (+)
RNA-Seq ExpressionClCG06G016480
SyntenyClCG06G016480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAACAACAAATATGAGATCTTCTTCTACAACACGGAAATGATTCGCATTCTGCTCCTACGAACAACCATAACCAGAAAAATTACCCACCGCCGGAGATGGAAGTTTTTGGGTTTATGCCAAACAAATGGATTGCAACCGCCCTTGGAATTTGGATTCAATGTATCTGCGGTGCTTCCTACACCTTCAGCATCTATTCTTCCGCTCTCAAGTCCTCCCAGGCCTACGATCAATCCACCCTCGACATCGTTTCCGTTTTCAAAGACATCGGCGCCAACGCCGGCATCATTTCTGGTTTTCTTTACTCCGCCGTCACTCCCCCAGCCCCCCGCTGGGCCTTTGCCGGGCCATGGGTGGTTCACGCCGCCGGAGCGATTCAGTGCTTCCTCGGATATATATTCATATGGGCCGCTATCTCTGGAGTGATCGATCGGCCGCCGGTGCCGGCGATGTGCTTCTTCATGTTTCTGGCTGCACATGCGCAAACGTTTTTCAATACGGCGAATGTGGTAACTGGCGTGCACAATTTTGCGCACTATAGCGGGACGATTGTGGGAATCATGAAGGTACCAACAAAAACCCTTGCTTATCTTCTTTATCATTATGCAGATTGTTAGGGCTTTGGATTTTACCTTTGATTTCTTCCCTTTTCTGAACCCTTATTGTATTTTTCTCTCTGGAAGGTTTGGTTATGGTGAGTACTATTTAGATACTGTAAAGAATTCATGTACTCTAAAATTAAATCTATGTCCTGGAGATGAACTTTGTTTAGCTGGCCTCATTCTTTACAATAGATTTATCTTGAAGCATAGAAATGGAAGAAGACGGTGGAGTCACCAAACCAATTTATTTTTCCTTCATGCTCTGTTCTTCTCTGCCATAATCTATCTTTGATCAAGGTGGTACCCAGATCAAAACTCCAAGTTATTGTCTCACGGGGGTCCTGGAATATGAGACTACACGTAAATGTAAACATAACCATAAAAATCAATCGTTTGATTATCAAACGAGTTCTAATTTATCAGAAAGCCTCGTGTTTAAGCCTAGTGTTATTTACTTTTTTCAATTAATGTTGATAATTACTTGTAAGTATTAGTTTTGTGCTAATCTCTAAACCCAGAAAGTGGTAGTGTTAAAACTATATATAGATTAAATATGTGTGGGACTATTAATTTAAAGGAAAAAACTTTGGTGCATCCTAATTGCACCTATCTTTGTACATTCCACCAAATTTGTCACATGGCAAATTCTTTTTACTTTTTTGAAATATTTTAATCATTTTTTATGCAGATGAGATTGGGATGCACAGGCATCTTGAAATACACCCAATATTGCTCTTAACTTAATAGGAGTTTAACAGTAACTTTTAGTGACTACATCTGATTCTCATTTATTCCGTGGAAAAGAAGGGATCACTATACGAATAGTTTCAAGGTTTTTTATTTGACAAAGTGGGGATTACTATTGCTTTTCTTTGGTGCGAAACTAAGCACCATTTGTCTCACTTCAACTTATCTTTTTTATTATAATCACTTTTTGGTTTCCCTATATTTCTTTCATTAATGAAATGTTTCTAATAAAAAAAATCAATAATAAATAAAGGGTTTTATTTGACAAAGTTGTAGTCGCCTTGTTGAATCTGAGAGCCGATTCTGAGGATCTTCCCCAAATTTTGTGTTGTCTTGAATGTGCTATTTATGAATTCATATCTTGTGTCTCCTTTTTGTTGGATAGGGATATCTTGGTCTCAGTGGAGCACTGCTAATCCAAGTTTATAATACGACTTGCAATGGAGATCCAAGCAATTTTCTCCTCATGCTGGCAGTTTTGCCCACACTTCTTTCCATCATGTTCATGTGGTTTGTTAGAATTGACAAAACAAAGTCTAGTAATGAGATGAAGCATTTGAATTCATTGTCTGCACTTGCTGTGATAGTTGCTTTTTACCTAATGGTTGTAATCATCTTGAACAACACTTTCTCCCTTTCATCATGGACTCGTTTCTTTACATTCTCCATACTCTTGATTCTCCTTGCTGCTCCTCTTGCAATCGCCATTAATGCTCAGAGGGAAGACTTTAGAGAGTCATCTCCATCATTCCTCACTGAAAAAAGTCATGTTGTTAACAAACTCAAGTCAATGGATGCAGAGGATTCTGTTGAATATCATGAGTTGCCCAGGGATGAGAGCCAGACAGTGGCTGTTTCCAACTCTAGAATTCCTCAAGCCATGAATGTCTTGGAAGCTATACGCACTATAAACTTCTGGCTGTTGTTTCTTGCCATGGTGTGTGGAATGGGTTCTGGATTGGCCACAATAAACAACATGAGCCAACTTGGCCAGTCTCTTGGTTACACAGCAACAGAGACGAAGACATTTGTTTCGTTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCTGGTTACACCTCTGATTTTCTTCTCCACTCATATGGGTGGGCAAGGCCATTGTTAATGGCAATTACTCTAATGATAATGAGTGGCGGCCACATCGTGATCGCCTCCGGTTTCTCTGGAAATCTGTATGTGGGTTCAATTCTTATTGGCATATGCTATGGCTCACAATGGTCATTGATGCCTACAATCACTTCAGAGATATTTGGTTTAGAGCATATGGGTACTATTTTCAACACCATAGCCATCGCCAGCCCTTTGGGGTCTTACATTTTTTCTGTTCGAGTAATCGGTTACATTTACGACAGAGAAGCTGCCGGAGAACATGGCTCCTGCTCTGGTACACACTGTTTGTTGATATCTTTTCTTGTCATGGCCGTCGTGGCTTTCTTGGGATCTCTTGTTGTTGCTGCACTCTTCTTCCGAACCAGGAGATTTTACCGACTGGCGTTGCAAAGAAGAGTGGAATGTCTCTAAGTTAGAGGTGCAAGAGAGAAAATTAGGATAAGTAAAGATGATTGCTTATAGTTTTTATAATATCCTTGAAGGATTTTGCTGAGTGTAAAGAAATTTGTTGTCTTGCTTCATTGAAGTTTTATACTTTTAAACAGAAAATCAGAGTGAGATCGAGTTTTATTTTTAATTTAAACGTTGTACCGTACTGTATGTATCGTAGGTTGATCAAGAGACCCGTCATTTTTCGTACATTCTTTTTTAATGAAATTATATCAGTTATTTTGAAGGGAA

mRNA sequence

GAACAACAAATATGAGATCTTCTTCTACAACACGGAAATGATTCGCATTCTGCTCCTACGAACAACCATAACCAGAAAAATTACCCACCGCCGGAGATGGAAGTTTTTGGGTTTATGCCAAACAAATGGATTGCAACCGCCCTTGGAATTTGGATTCAATGTATCTGCGGTGCTTCCTACACCTTCAGCATCTATTCTTCCGCTCTCAAGTCCTCCCAGGCCTACGATCAATCCACCCTCGACATCGTTTCCGTTTTCAAAGACATCGGCGCCAACGCCGGCATCATTTCTGGTTTTCTTTACTCCGCCGTCACTCCCCCAGCCCCCCGCTGGGCCTTTGCCGGGCCATGGGTGGTTCACGCCGCCGGAGCGATTCAGTGCTTCCTCGGATATATATTCATATGGGCCGCTATCTCTGGAGTGATCGATCGGCCGCCGGTGCCGGCGATGTGCTTCTTCATGTTTCTGGCTGCACATGCGCAAACGTTTTTCAATACGGCGAATGTGGTAACTGGCGTGCACAATTTTGCGCACTATAGCGGGACGATTGTGGGAATCATGAAGGGATATCTTGGTCTCAGTGGAGCACTGCTAATCCAAGTTTATAATACGACTTGCAATGGAGATCCAAGCAATTTTCTCCTCATGCTGGCAGTTTTGCCCACACTTCTTTCCATCATGTTCATGTGGTTTGTTAGAATTGACAAAACAAAGTCTAGTAATGAGATGAAGCATTTGAATTCATTGTCTGCACTTGCTGTGATAGTTGCTTTTTACCTAATGGTTGTAATCATCTTGAACAACACTTTCTCCCTTTCATCATGGACTCGTTTCTTTACATTCTCCATACTCTTGATTCTCCTTGCTGCTCCTCTTGCAATCGCCATTAATGCTCAGAGGGAAGACTTTAGAGAGTCATCTCCATCATTCCTCACTGAAAAAAGTCATGTTGTTAACAAACTCAAGTCAATGGATGCAGAGGATTCTGTTGAATATCATGAGTTGCCCAGGGATGAGAGCCAGACAGTGGCTGTTTCCAACTCTAGAATTCCTCAAGCCATGAATGTCTTGGAAGCTATACGCACTATAAACTTCTGGCTGTTGTTTCTTGCCATGGTGTGTGGAATGGGTTCTGGATTGGCCACAATAAACAACATGAGCCAACTTGGCCAGTCTCTTGGTTACACAGCAACAGAGACGAAGACATTTGTTTCGTTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCTGGTTACACCTCTGATTTTCTTCTCCACTCATATGGGTGGGCAAGGCCATTGTTAATGGCAATTACTCTAATGATAATGAGTGGCGGCCACATCGTGATCGCCTCCGGTTTCTCTGGAAATCTGTATGTGGGTTCAATTCTTATTGGCATATGCTATGGCTCACAATGGTCATTGATGCCTACAATCACTTCAGAGATATTTGGTTTAGAGCATATGGGTACTATTTTCAACACCATAGCCATCGCCAGCCCTTTGGGGTCTTACATTTTTTCTGTTCGAGTAATCGGTTACATTTACGACAGAGAAGCTGCCGGAGAACATGGCTCCTGCTCTGGTACACACTGTTTGTTGATATCTTTTCTTGTCATGGCCGTCGTGGCTTTCTTGGGATCTCTTGTTGTTGCTGCACTCTTCTTCCGAACCAGGAGATTTTACCGACTGGCGTTGCAAAGAAGAGTGGAATGTCTCTAAGTTAGAGGTGCAAGAGAGAAAATTAGGATAAGTAAAGATGATTGCTTATAGTTTTTATAATATCCTTGAAGGATTTTGCTGAGTGTAAAGAAATTTGTTGTCTTGCTTCATTGAAGTTTTATACTTTTAAACAGAAAATCAGAGTGAGATCGAGTTTTATTTTTAATTTAAACGTTGTACCGTACTGTATGTATCGTAGGTTGATCAAGAGACCCGTCATTTTTCGTACATTCTTTTTTAATGAAATTATATCAGTTATTTTGAAGGGAA

Coding sequence (CDS)

ATGGAAGTTTTTGGGTTTATGCCAAACAAATGGATTGCAACCGCCCTTGGAATTTGGATTCAATGTATCTGCGGTGCTTCCTACACCTTCAGCATCTATTCTTCCGCTCTCAAGTCCTCCCAGGCCTACGATCAATCCACCCTCGACATCGTTTCCGTTTTCAAAGACATCGGCGCCAACGCCGGCATCATTTCTGGTTTTCTTTACTCCGCCGTCACTCCCCCAGCCCCCCGCTGGGCCTTTGCCGGGCCATGGGTGGTTCACGCCGCCGGAGCGATTCAGTGCTTCCTCGGATATATATTCATATGGGCCGCTATCTCTGGAGTGATCGATCGGCCGCCGGTGCCGGCGATGTGCTTCTTCATGTTTCTGGCTGCACATGCGCAAACGTTTTTCAATACGGCGAATGTGGTAACTGGCGTGCACAATTTTGCGCACTATAGCGGGACGATTGTGGGAATCATGAAGGGATATCTTGGTCTCAGTGGAGCACTGCTAATCCAAGTTTATAATACGACTTGCAATGGAGATCCAAGCAATTTTCTCCTCATGCTGGCAGTTTTGCCCACACTTCTTTCCATCATGTTCATGTGGTTTGTTAGAATTGACAAAACAAAGTCTAGTAATGAGATGAAGCATTTGAATTCATTGTCTGCACTTGCTGTGATAGTTGCTTTTTACCTAATGGTTGTAATCATCTTGAACAACACTTTCTCCCTTTCATCATGGACTCGTTTCTTTACATTCTCCATACTCTTGATTCTCCTTGCTGCTCCTCTTGCAATCGCCATTAATGCTCAGAGGGAAGACTTTAGAGAGTCATCTCCATCATTCCTCACTGAAAAAAGTCATGTTGTTAACAAACTCAAGTCAATGGATGCAGAGGATTCTGTTGAATATCATGAGTTGCCCAGGGATGAGAGCCAGACAGTGGCTGTTTCCAACTCTAGAATTCCTCAAGCCATGAATGTCTTGGAAGCTATACGCACTATAAACTTCTGGCTGTTGTTTCTTGCCATGGTGTGTGGAATGGGTTCTGGATTGGCCACAATAAACAACATGAGCCAACTTGGCCAGTCTCTTGGTTACACAGCAACAGAGACGAAGACATTTGTTTCGTTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCTGGTTACACCTCTGATTTTCTTCTCCACTCATATGGGTGGGCAAGGCCATTGTTAATGGCAATTACTCTAATGATAATGAGTGGCGGCCACATCGTGATCGCCTCCGGTTTCTCTGGAAATCTGTATGTGGGTTCAATTCTTATTGGCATATGCTATGGCTCACAATGGTCATTGATGCCTACAATCACTTCAGAGATATTTGGTTTAGAGCATATGGGTACTATTTTCAACACCATAGCCATCGCCAGCCCTTTGGGGTCTTACATTTTTTCTGTTCGAGTAATCGGTTACATTTACGACAGAGAAGCTGCCGGAGAACATGGCTCCTGCTCTGGTACACACTGTTTGTTGATATCTTTTCTTGTCATGGCCGTCGTGGCTTTCTTGGGATCTCTTGTTGTTGCTGCACTCTTCTTCCGAACCAGGAGATTTTACCGACTGGCGTTGCAAAGAAGAGTGGAATGTCTCTAA

Protein sequence

MEVFGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGANAGIISGFLYSAVTPPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCFFMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLLMLAVLPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSLSSWTRFFTFSILLILLAAPLAIAINAQREDFRESSPSFLTEKSHVVNKLKSMDAEDSVEYHELPRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIASGFSGNLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQRRVECL
Homology
BLAST of ClCG06G016480 vs. NCBI nr
Match: XP_038879631.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 966.8 bits (2498), Expect = 7.6e-278
Identity = 496/542 (91.51%), Postives = 519/542 (95.76%), Query Frame = 0

Query: 1   MEVFGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGAN 60
           ME+ GFMPNKWIATALGIWIQCICGASYTFSIYS ALKS+QAYDQSTLD VSVFKDIGAN
Sbjct: 1   MEISGFMPNKWIATALGIWIQCICGASYTFSIYSPALKSTQAYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCF 120
           AGIISGFLYSAVT PAPR  FAGPWVVHAAGAIQ FLGYIFIWAA+SGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTTPAPR-PFAGPWVVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNF HYSGT+VGIMKGYLGLSGAL+IQVY TTCNGDPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFEHYSGTVVGIMKGYLGLSGALIIQVY-TTCNGDPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAVLPTLLSIMFMWFVRIDKT+SSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL
Sbjct: 181 FLLMLAVLPTLLSIMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLAIAINAQREDFRESSPSFLTEKSHVVNKLKSMDAEDSVEY 300
           S+WTRFFTFSILLILLAAPL IAINAQ+EDFRE SPSF++EKS  VNK +SMDAEDSVEY
Sbjct: 241 SAWTRFFTFSILLILLAAPLGIAINAQKEDFREPSPSFISEKSQ-VNKSESMDAEDSVEY 300

Query: 301 HELPRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELPR+E+Q +AV+NSR PQ +NVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQ+GQS
Sbjct: 301 HELPREENQIMAVANSRTPQPLNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQIGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITL+ MSGGHI+IA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLGMSGGHIMIA 420

Query: 421 SGFSGNLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSIL+GICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQRRVE 540
           IGYIYDREA  EHGSC+GTHC ++SFLVMAVVAF GSLV AALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREATEEHGSCNGTHCFVVSFLVMAVVAFFGSLVAAALFFRTRRFYRLALQRRVE 539

Query: 541 CL 543
           CL
Sbjct: 541 CL 539

BLAST of ClCG06G016480 vs. NCBI nr
Match: XP_008453166.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo] >KAA0057917.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ98605.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 965.7 bits (2495), Expect = 1.7e-277
Identity = 491/542 (90.59%), Postives = 513/542 (94.65%), Query Frame = 0

Query: 1   MEVFGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGAN 60
           ME   FM NKW AT LGIWIQCICGASYTFSIYSSALKS+Q+YDQSTLD VSVFKDIGAN
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCF 120
           AGIISGFLYSAVTP  PR AFAGPW+VHAAGAIQ FLGYIFIWAA+SGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFA+YSGTIVGIMKGYLGLSGALLIQVYNTTCN DPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAVLPT+LSIMFMWFVRIDKT+S NEMKHLNSLSALAVIVAFYLMVVIILNN FSL
Sbjct: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLAIAINAQREDFRESSPSFLTEKSHVVNKLKSMDAEDSVEY 300
           SSWTR+FTFSILLILLAAPL IAINAQ+EDFR S PS +TE+SHVVNK + +DAEDSVEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEY 300

Query: 301 HELPRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELPR+E Q + VSNSR PQ MNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPREEDQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFL H+YGWARPLLMAITL+IMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSIL+GICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAAGEHG+CSGTHC ++SFLVMA+VAFLG LV AALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CL 543
           CL
Sbjct: 541 CL 542

BLAST of ClCG06G016480 vs. NCBI nr
Match: XP_022972528.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 957.6 bits (2474), Expect = 4.6e-275
Identity = 484/542 (89.30%), Postives = 513/542 (94.65%), Query Frame = 0

Query: 1   MEVFGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGAN 60
           MEV GFM NKW+ATA+GIWIQCICGASYTFSIYSSALKS+Q YDQSTLD VSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCF 120
           AG+ISGFLYSAVTP + R +  GPWVVHAAGAIQCFLGYIFIWAA+SG+IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPASRRRSSVGPWVVHAAGAIQCFLGYIFIWAAVSGLIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMKGYLGLSGALLIQVYNT CNGDPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMKGYLGLSGALLIQVYNTACNGDPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           F+LMLAV PTLLSIMFMWFVRID+T+S NEMKHLNSLSALAVIVAFYLMVVII NNTFSL
Sbjct: 181 FVLMLAVFPTLLSIMFMWFVRIDRTESRNEMKHLNSLSALAVIVAFYLMVVIIFNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLAIAINAQREDFRESSPSFLTEKSHVVNKLKSMDAEDSVEY 300
           SSWTRF TFSILL+LL APL IAINAQREDFR SSPSF TE++ V+NK KS +AEDSVEY
Sbjct: 241 SSWTRFVTFSILLVLLVAPLGIAINAQREDFRMSSPSFFTEENPVLNKPKSANAEDSVEY 300

Query: 301 HELPRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP DE QT+A +NSR P+AMNVLEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPSDELQTMAAANSRAPRAMNVLEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITL+IMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSIL+GICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAA +HGSCSGTHC ++SFLVMA+VAFLGSLV AALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAEKHGSCSGTHCFVVSFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CL 543
            L
Sbjct: 541 RL 542

BLAST of ClCG06G016480 vs. NCBI nr
Match: XP_023531919.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 954.5 bits (2466), Expect = 3.9e-274
Identity = 483/542 (89.11%), Postives = 512/542 (94.46%), Query Frame = 0

Query: 1   MEVFGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGAN 60
           MEV GFM NKW+ATA+GIWIQCICGASYTFSIYSSALKS+Q YDQSTLD VSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCF 120
           AG+ISGFLYSAVTP A R +  GPWVVHAAGAIQCFLGYIFIWAA+SG+IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPAARRRSSLGPWVVHAAGAIQCFLGYIFIWAAVSGLIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMKGYLGLSGALLIQVYNT CNG+PSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMKGYLGLSGALLIQVYNTACNGEPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAV PTLLSIMFMWFVRID+T+S NEMKHLNSLSALAVIVAFY+MVVII NNTFSL
Sbjct: 181 FLLMLAVFPTLLSIMFMWFVRIDRTESRNEMKHLNSLSALAVIVAFYVMVVIIFNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLAIAINAQREDFRESSPSFLTEKSHVVNKLKSMDAEDSVEY 300
           SSWTRF TFSILL+LL APL IAINAQRED R SSPSF TE++ V+NK KS +AEDSVEY
Sbjct: 241 SSWTRFVTFSILLVLLVAPLGIAINAQREDLRMSSPSFFTEENPVLNKPKSANAEDSVEY 300

Query: 301 HELPRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP DE QT+A +NSR P+AMNVLEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPSDELQTMAAANSRAPRAMNVLEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITL+IMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSIL+GICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAA +HGSCSGTHC ++SFLVMA+VAFLGSLV AALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAEKHGSCSGTHCFVVSFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CL 543
            L
Sbjct: 541 RL 542

BLAST of ClCG06G016480 vs. NCBI nr
Match: KAG7021495.1 (Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 950.7 bits (2456), Expect = 5.6e-273
Identity = 482/542 (88.93%), Postives = 509/542 (93.91%), Query Frame = 0

Query: 1   MEVFGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGAN 60
           MEV GFM NKW+ATA+GIWIQCICGASYTFSIYSSALKS+Q YDQSTLD VSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCF 120
           AG+ISGFLYSAVTP   R + AGPWVVHAAGAIQCFLGYIFIWAA+SG IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPTTRRRSSAGPWVVHAAGAIQCFLGYIFIWAAVSGFIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMKGYLGLSGALLIQVYNT CNG+PSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMKGYLGLSGALLIQVYNTACNGEPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAV PTLLSI FMWFVRID+T+S NEMKHLNSLSALAVIVAFYLMVVII NNTFSL
Sbjct: 181 FLLMLAVFPTLLSITFMWFVRIDRTESRNEMKHLNSLSALAVIVAFYLMVVIIFNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLAIAINAQREDFRESSPSFLTEKSHVVNKLKSMDAEDSVEY 300
           SSWTRF TFSILL+LL APL IAINAQRED R SSPSF TE++ V+NK KS +AEDSVEY
Sbjct: 241 SSWTRFVTFSILLVLLVAPLGIAINAQREDLRMSSPSFFTEENPVLNKPKSANAEDSVEY 300

Query: 301 HELPRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP DE QT+A +NSR P+AMNVLEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPSDELQTMAAANSRAPRAMNVLEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITL+IMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SG SGNLYVGSIL+GICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGLSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAA +HGSCSGTHC ++SFLVMA+VAFLGSLV AALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAEKHGSCSGTHCFVVSFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CL 543
            L
Sbjct: 541 RL 542

BLAST of ClCG06G016480 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 1.0e-27
Identity = 144/556 (25.90%), Postives = 243/556 (43.71%), Query Frame = 0

Query: 10  KWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGANAGIISGFLY 69
           KW      IWIQ   G ++ FS YSS LKS     Q  L+ ++V  D+G   G  SG   
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 70  SAVTPPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCFFMFLAAHAQ 129
                      +    VV  A A   F+GY   W  I+ +I   P   +     LA  + 
Sbjct: 103 ----------GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITL-PYSLVFLCCLLAGLSI 162

Query: 130 TFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLLMLAVLP 189
            +FNTA  +  + +F +     + +   + G+S AL    +N       + +LL+ +++P
Sbjct: 163 CWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVP 222

Query: 190 TLLSIMFMWFV----RIDKTKSSNEMKH----LNSLSALAVIVAFYLMVVIILNNTFSLS 249
            ++S   ++ V     +D T   +  +H       L+ LAVI +F+L    +L+++ + S
Sbjct: 223 LVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHL----LLSSSSTSS 282

Query: 250 SWTRFFTFSILLIL-LAAPLAI--------AINAQREDFRESSPSFLTEKSHVVNKLKSM 309
           +   F    +LL+  L APL +         INA+     ESS   +     + N+  S+
Sbjct: 283 ARLNFIGAVVLLVFPLCAPLLVYARDYFLPVINARLN--HESSGYVMLNIDELKNQKTSV 342

Query: 310 DAEDSVEYHELPRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATIN 369
            ++   E H     E  TV + +    + +     I  + FWL ++A  CG   GL   N
Sbjct: 343 SSKTGYE-HMGTAKEGNTVRLGDEHSFRLL-----ISRLEFWLYYIAYFCGGTIGLVYSN 402

Query: 370 NMSQLGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIM 429
           N+ Q+ QSLG  +T   T V+++S ++F GR       DF+   +   R    AI L+  
Sbjct: 403 NLGQIAQSLGQNST---TLVTIYSSFSFFGRL-LSAAPDFMHKRFRLTRTGWFAIALLPT 462

Query: 430 SGGHIVIASGFSGN--LYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASP 489
                ++A   S    L   + LIG+  G  ++   +ITS++FG   +G   N +    P
Sbjct: 463 PIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIP 522

Query: 490 LGSYIFSVRVIGYIYDREAA-------GEHGSCSGTHCLLISFLVMAVVAFLGSLVVAAL 540
           +GS ++   +   IY+  A+        +   C G  C   +F+    ++ LG +   +L
Sbjct: 523 IGSLLYGY-IAASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSL 570

BLAST of ClCG06G016480 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 2.2e-09
Identity = 56/206 (27.18%), Postives = 109/206 (52.91%), Query Frame = 0

Query: 334 WLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTF---VSLWSIWNFLGRFGAGYTS 393
           WL  L  V   G      NNM  +  ++     ++ +F   VSL++ ++ + R   G++S
Sbjct: 275 WLFLLCFVFIGGPFEMFQNNMGAILDTVTVENADSPSFSTHVSLFATFSTVSRLVVGFSS 334

Query: 394 DFLLHSYGWARPLLMAITLMIMSGGHIVIASG----FSGNLY--VGSILIGICYGSQWSL 453
           + +      +RP+L+++  ++ +  H+++ SG    F    Y  V +I+ G  YGS ++L
Sbjct: 335 EAMESHV--SRPVLLSVIALVAACIHLMVPSGIFTVFDNAKYFSVVTIVNGFSYGSSFTL 394

Query: 454 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEHGS----CSGTH 513
           +PTI ++++G+ ++GTI+ +  +A  +GS  + + +   +YD  +    GS    CSG H
Sbjct: 395 VPTIVTKVWGIANLGTIWGSFILALAVGSLGYGL-LFAKVYDAASEVGVGSMSQVCSGVH 454

Query: 514 CLLISFLVMAV-VAFLGSLVVAALFF 526
           C  ++F++    +AF      AA+FF
Sbjct: 455 CYGLTFVITGTGLAF----AAAAVFF 473

BLAST of ClCG06G016480 vs. ExPASy Swiss-Prot
Match: Q96TW9 (Probable transporter MCH1 OS=Wickerhamomyces anomalus OX=4927 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.4e-08
Identity = 51/185 (27.57%), Postives = 92/185 (49.73%), Query Frame = 0

Query: 325 LEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 384
           L+ I+ I+ ++L  +++  +G     I NM  L +++   +  +   V++ ++++ L R 
Sbjct: 256 LKFIKDISTYVLLFSLLLSIGPSEMYITNMGSLVKAITPNSLISDQ-VAIHAVFSTLSRL 315

Query: 385 GAGYTSDFLLHSYGWARP--LLMAITLMIMSGGHIVIASGFSGNLYVGSILIGICYGSQW 444
             G  SDFL+ +Y  +R   LL  I L   +   I  ++      Y+ S L G  YG  +
Sbjct: 316 SLGALSDFLVTNYQISRSWLLLSIIVLGFFTQIFIATSTFVKDQYYIISALSGFSYGGLF 375

Query: 445 SLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEHGSCSGTHCL 504
           +L PT+   I+G E  G+ + +  IA  +GS  F + V G +YD  A G     +  +C+
Sbjct: 376 TLYPTVIFSIWGPEIFGSAWGSFMIAPAIGSTTFGM-VFGLVYD-SACGVFAESTTGNCV 435

Query: 505 LISFL 508
            + FL
Sbjct: 436 SLVFL 437

BLAST of ClCG06G016480 vs. ExPASy Swiss-Prot
Match: Q6FWD4 (Probable transporter MCH1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 3.8e-06
Identity = 60/253 (23.72%), Postives = 112/253 (44.27%), Query Frame = 0

Query: 294 AEDSVEYHELPRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINN 353
           +++ VE H    ++S+ +  +++   Q M +++  R    ++    + C +G     I N
Sbjct: 244 SDNDVENHYDDNEQSRLLHATHA---QQMTLMKVFRDPVLYIFGATIFCALGPLEMFIAN 303

Query: 354 MSQLGQSL--GYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLH---SYGWARPLLMAIT 413
           M  L   L  G+    +   +S++++ + L R G G T D+      S  W   L + + 
Sbjct: 304 MGSLTNVLAGGHEPAMSSALLSIYALTSTLTRLGTGLTVDYFNKRQLSVKWILLLFLVVG 363

Query: 414 LMIMSGGHIVIASGFSGN---------LYVGSILIGICYGSQWSLMPTITSEIFGLEHMG 473
           L+     +++  S    +          Y+G I+ GI YG  +++ PTIT  ++G +  G
Sbjct: 364 LVTQGKIYMLSMSSLDHSHMVTINRKLFYIG-IMQGIAYGGLFTIYPTITLMVWGEKMFG 423

Query: 474 TIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEHGSCSGTHCLLISFLVMAVVAFLGSL- 530
           T + T+ IA  LGS + S  +   +YD E A      S T   +        + F  ++ 
Sbjct: 424 TAYGTLMIAPALGSAL-SCLIYADVYDSECAN-----STTRSCIAPVYETTALEFCAAIL 483

BLAST of ClCG06G016480 vs. ExPASy TrEMBL
Match: A0A5A7UT75 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002270 PE=4 SV=1)

HSP 1 Score: 965.7 bits (2495), Expect = 8.2e-278
Identity = 491/542 (90.59%), Postives = 513/542 (94.65%), Query Frame = 0

Query: 1   MEVFGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGAN 60
           ME   FM NKW AT LGIWIQCICGASYTFSIYSSALKS+Q+YDQSTLD VSVFKDIGAN
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCF 120
           AGIISGFLYSAVTP  PR AFAGPW+VHAAGAIQ FLGYIFIWAA+SGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFA+YSGTIVGIMKGYLGLSGALLIQVYNTTCN DPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAVLPT+LSIMFMWFVRIDKT+S NEMKHLNSLSALAVIVAFYLMVVIILNN FSL
Sbjct: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLAIAINAQREDFRESSPSFLTEKSHVVNKLKSMDAEDSVEY 300
           SSWTR+FTFSILLILLAAPL IAINAQ+EDFR S PS +TE+SHVVNK + +DAEDSVEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEY 300

Query: 301 HELPRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELPR+E Q + VSNSR PQ MNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPREEDQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFL H+YGWARPLLMAITL+IMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSIL+GICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAAGEHG+CSGTHC ++SFLVMA+VAFLG LV AALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CL 543
           CL
Sbjct: 541 CL 542

BLAST of ClCG06G016480 vs. ExPASy TrEMBL
Match: A0A1S3BWC2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 SV=1)

HSP 1 Score: 965.7 bits (2495), Expect = 8.2e-278
Identity = 491/542 (90.59%), Postives = 513/542 (94.65%), Query Frame = 0

Query: 1   MEVFGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGAN 60
           ME   FM NKW AT LGIWIQCICGASYTFSIYSSALKS+Q+YDQSTLD VSVFKDIGAN
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCF 120
           AGIISGFLYSAVTP  PR AFAGPW+VHAAGAIQ FLGYIFIWAA+SGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFA+YSGTIVGIMKGYLGLSGALLIQVYNTTCN DPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAVLPT+LSIMFMWFVRIDKT+S NEMKHLNSLSALAVIVAFYLMVVIILNN FSL
Sbjct: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLAIAINAQREDFRESSPSFLTEKSHVVNKLKSMDAEDSVEY 300
           SSWTR+FTFSILLILLAAPL IAINAQ+EDFR S PS +TE+SHVVNK + +DAEDSVEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEY 300

Query: 301 HELPRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELPR+E Q + VSNSR PQ MNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPREEDQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFL H+YGWARPLLMAITL+IMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSIL+GICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAAGEHG+CSGTHC ++SFLVMA+VAFLG LV AALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CL 543
           CL
Sbjct: 541 CL 542

BLAST of ClCG06G016480 vs. ExPASy TrEMBL
Match: A0A6J1IA87 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471070 PE=4 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 2.2e-275
Identity = 484/542 (89.30%), Postives = 513/542 (94.65%), Query Frame = 0

Query: 1   MEVFGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGAN 60
           MEV GFM NKW+ATA+GIWIQCICGASYTFSIYSSALKS+Q YDQSTLD VSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCF 120
           AG+ISGFLYSAVTP + R +  GPWVVHAAGAIQCFLGYIFIWAA+SG+IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPASRRRSSVGPWVVHAAGAIQCFLGYIFIWAAVSGLIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMKGYLGLSGALLIQVYNT CNGDPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMKGYLGLSGALLIQVYNTACNGDPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           F+LMLAV PTLLSIMFMWFVRID+T+S NEMKHLNSLSALAVIVAFYLMVVII NNTFSL
Sbjct: 181 FVLMLAVFPTLLSIMFMWFVRIDRTESRNEMKHLNSLSALAVIVAFYLMVVIIFNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLAIAINAQREDFRESSPSFLTEKSHVVNKLKSMDAEDSVEY 300
           SSWTRF TFSILL+LL APL IAINAQREDFR SSPSF TE++ V+NK KS +AEDSVEY
Sbjct: 241 SSWTRFVTFSILLVLLVAPLGIAINAQREDFRMSSPSFFTEENPVLNKPKSANAEDSVEY 300

Query: 301 HELPRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP DE QT+A +NSR P+AMNVLEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPSDELQTMAAANSRAPRAMNVLEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITL+IMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSIL+GICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAA +HGSCSGTHC ++SFLVMA+VAFLGSLV AALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAEKHGSCSGTHCFVVSFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CL 543
            L
Sbjct: 541 RL 542

BLAST of ClCG06G016480 vs. ExPASy TrEMBL
Match: A0A6J1F604 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111441144 PE=4 SV=1)

HSP 1 Score: 949.1 bits (2452), Expect = 7.9e-273
Identity = 481/542 (88.75%), Postives = 508/542 (93.73%), Query Frame = 0

Query: 1   MEVFGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGAN 60
           MEV GFM NKW+ATA+GIWIQCICGASYTFSIYSSALKS+Q YDQSTLD VSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCF 120
           AG+ISGFLYSAVTP   R + AGPWVVHAAGAIQCFLGYIFIWAA+SG IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPTTRRRSSAGPWVVHAAGAIQCFLGYIFIWAAVSGFIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMKGYLGLSGALLIQVYNT CNG+PSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMKGYLGLSGALLIQVYNTACNGEPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAV PTLLSI FMWFVRID+T+S NEMKHLNSLSALAVIVAFYLMV II NNTFSL
Sbjct: 181 FLLMLAVFPTLLSITFMWFVRIDRTESRNEMKHLNSLSALAVIVAFYLMVAIIFNNTFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLAIAINAQREDFRESSPSFLTEKSHVVNKLKSMDAEDSVEY 300
           SSWTRF TFSILL+LL APL IAINAQRED R SSPSF TE++ V+NK KS +AEDSVEY
Sbjct: 241 SSWTRFVTFSILLVLLVAPLGIAINAQREDLRMSSPSFFTEENPVLNKPKSANAEDSVEY 300

Query: 301 HELPRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELP DE QT+A +NSR P+AMNVLEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPSDELQTMAAANSRAPRAMNVLEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIA 420
           LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITL+IMSGGHIVIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SG SGNLYVGSIL+GICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGLSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQRRVE 540
           IGYIYDREAA +HGSCSGTHC ++SFLVMA+VAFLGSLV AALFFRTRRFYRLALQRRVE
Sbjct: 481 IGYIYDREAAEKHGSCSGTHCFVVSFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVE 540

Query: 541 CL 543
            L
Sbjct: 541 RL 542

BLAST of ClCG06G016480 vs. ExPASy TrEMBL
Match: A0A0A0LUE8 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G008470 PE=4 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 5.1e-272
Identity = 482/539 (89.42%), Postives = 508/539 (94.25%), Query Frame = 0

Query: 1   MEVFGFMPNKWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGAN 60
           M+   FM NKWIAT LGIWIQCICGASYTFSIYSSALKS+Q+YDQSTLD VSVFKDIGAN
Sbjct: 1   MDPSNFMSNKWIATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGIISGFLYSAVTPPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCF 120
           AGIISGFLYSAVTP  PR AFAGPW+VHAAGAIQ FLGYIFIWAA+SGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPFNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSN 180
           FMFLAAHAQTFFNTANVVTGVHNFA+YSGTIVGIMKGYLGLSGALLIQVYNTTCN DPSN
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNEDPSN 180

Query: 181 FLLMLAVLPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSL 240
           FLLMLAVLPT+LS+MFMWFVRIDKT+SSNEMKHLNSLSALAVIVAFYLMVVIILNN FSL
Sbjct: 181 FLLMLAVLPTVLSVMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 241 SSWTRFFTFSILLILLAAPLAIAINAQREDFRESSPSFLTEKSHVVNKLKSMDAEDSVEY 300
           SSWTR+FTFSILLILLAAPL IAINAQ+EDFR SS S + EKSHVVNK +S+DAEDSVEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSSSSLIAEKSHVVNKPESIDAEDSVEY 300

Query: 301 HELPRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360
           HELPR+E+Q + VSN+R PQ MNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS
Sbjct: 301 HELPREENQIMVVSNTRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIA 420
           LGYT TETKTFVSLWSIWNFLGRFGAGYTSDFL H+YGWARPLLMAITL+IMSGGHIVIA
Sbjct: 361 LGYTETETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 421 SGFSGNLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480
           SGFSGNLYVGSIL+GICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 481 IGYIYDREAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQRRV 540
           IGYIYDREAA EHG+CSG HC ++SF VMA+VAFLG LV AALFFRTRRFY+LA QRRV
Sbjct: 481 IGYIYDREAAREHGACSGIHCFVVSFFVMAIVAFLGFLVAAALFFRTRRFYQLASQRRV 539

BLAST of ClCG06G016480 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 614.4 bits (1583), Expect = 8.9e-176
Identity = 313/536 (58.40%), Postives = 400/536 (74.63%), Query Frame = 0

Query: 10  KWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGANAGIISGFLY 69
           KW+A    IWIQC  GASYTF IYS+ LKS+Q+YDQSTLD VSVFKDIGANAG+ SG LY
Sbjct: 7   KWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVFSGLLY 66

Query: 70  SAVTPPAPR------WAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCFFMF 129
           +  T    R          GPWVV A GAIQCF GY  IWA+++G+I +PPVP MC FMF
Sbjct: 67  TYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLMCLFMF 126

Query: 130 LAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLL 189
           LAA +QTFFNTANVV+ V NFA Y GT VGIMKG+LGLSGA+LIQ+Y T C GDP++F+L
Sbjct: 127 LAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDPASFIL 186

Query: 190 MLAVLPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSLSSW 249
           +LAV PT+LS++ M  VRI +T  +++ KHLN LSA+++I+A YLM++IIL NTF LSSW
Sbjct: 187 LLAVTPTVLSLLVMPLVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTFGLSSW 246

Query: 250 TRFFTFSILLILLAAPLAIAINAQREDFRESSPSFLTEKSHVVNKLKSMDAEDSVEYHEL 309
               T   LL++LA PL IA  AQR+   ++ P    + S +++  K+  + +       
Sbjct: 247 ANIVTLVCLLVMLALPLLIARRAQRDGMEKTVPH---DYSPLISSPKATTSGN------- 306

Query: 310 PRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGY 369
               S+  +   + + + +N+L+A++ ++FWLLFLAM+CGMGSGL+TINN+ Q+G+SL Y
Sbjct: 307 --QSSEGDSKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRY 366

Query: 370 TATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIASGF 429
           ++ E  + VSLWSIWNFLGRFGAGY SD LLH  GW RPLLMA TL  MS GH++IASGF
Sbjct: 367 SSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGF 426

Query: 430 SGNLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGY 489
            GNLYVGS+++G+CYGSQWSLMPTITSE+FG+ HMGTIFNTI++ASP+GSYIFSVR+IGY
Sbjct: 427 QGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGY 486

Query: 490 IYDREAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQRRV 540
           IYD+ A+GE  +C G+HC  +SF++MA VAF G LV   LFFRT+  YR  L +R+
Sbjct: 487 IYDKTASGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYRQILVKRL 530

BLAST of ClCG06G016480 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 568.9 bits (1465), Expect = 4.3e-162
Identity = 290/532 (54.51%), Postives = 382/532 (71.80%), Query Frame = 0

Query: 10  KWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGANAGIISGFLY 69
           KW+A    IWIQC  G SYTF IYS+ LKS+Q+YDQSTLD VSVFKDIG N G++SG +Y
Sbjct: 9   KWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVLSGLVY 68

Query: 70  SAVT----PPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCFFMFLA 129
           +A T        R    GPWVV   GAI  F GY  +WA+++G+I RPPVP MC FMF+A
Sbjct: 69  TAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCLFMFIA 128

Query: 130 AHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLLML 189
           A + TF NTANVV+ + NFA Y GT VGIMKG++GLSGA+LIQ+Y   C GDP  F+L+L
Sbjct: 129 AQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKTFILLL 188

Query: 190 AVLPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSLSSWTR 249
           A++P+LLS++ M  VR+ KT + +E KHL+ LS L++I+A YLM+ IIL +T SL SW  
Sbjct: 189 AIVPSLLSVLVMPLVRVYKTSTVDEKKHLDGLSTLSLIIAAYLMITIILKSTLSLPSWAN 248

Query: 250 FFTFSILLILLAAPLAIAINAQREDFRESSPSFLTEKSHVVNKLKSMDAEDSVEYHELPR 309
             T ++LL+LL++PL +A+ A R+   +   S  +        + +++A  S E   L  
Sbjct: 249 AVTLAVLLVLLSSPLLVAVRAHRDSIEKPLSSVYSP------LVDNLEATTSGEILMLDE 308

Query: 310 DESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTA 369
           D+S             +N+L+A+  ++FWLLFLAM+CGMGSG++TINN+ Q+G+SL YT+
Sbjct: 309 DKS-------------LNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGESLRYTS 368

Query: 370 TETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIASGFSG 429
            E  + ++LW+IWNF+GRFG GY SD+LLH  GW RPLLMA TL  M+ GH++IASGF G
Sbjct: 369 VEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLIIASGFQG 428

Query: 430 NLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIY 489
           NLY GSI++GICYGSQWSLMPTITSE+FG++HMGTI+NTI+IASP+GSYIFSVR+IGYIY
Sbjct: 429 NLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSVRLIGYIY 488

Query: 490 DREAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQR 538
           DR   GE  +C G HC  ++++V+A VAFLG LV   L FRT+  YR   ++
Sbjct: 489 DRTIIGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIYRQIFEK 521

BLAST of ClCG06G016480 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 568.5 bits (1464), Expect = 5.6e-162
Identity = 302/538 (56.13%), Postives = 382/538 (71.00%), Query Frame = 0

Query: 6   FMPNKWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGANAGIIS 65
           F   KW+A A  IWIQ   GASYTF IYSS LKSSQ+YDQSTLD VSV+KDIGAN GI+S
Sbjct: 3   FANTKWVAAAASIWIQSFSGASYTFGIYSSVLKSSQSYDQSTLDTVSVYKDIGANVGILS 62

Query: 66  GFLYSAVT--PPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCFFMF 125
           G  Y+AV          F+GPW+V   G +Q F+GY FIW A SGVI RPPV  MC FMF
Sbjct: 63  GLFYTAVASRKSGNGGFFSGPWLVIFVGLLQWFVGYGFIWMATSGVIPRPPVAMMCLFMF 122

Query: 126 LAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLL 185
            A H Q FFNTA VVT V NF+ Y GT VGIMKGYLGLSGA+L+Q+Y+  C GDP N++L
Sbjct: 123 FAGHCQPFFNTAIVVTAVRNFSDYGGTAVGIMKGYLGLSGAILVQMYHIFCGGDPRNYIL 182

Query: 186 MLAVLPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSLSSW 245
           +LAV+P+LL +  M FVR   T  + + KHLN LSA+++I+  YLMVVI++ N   +S  
Sbjct: 183 LLAVVPSLLILTLMPFVRTYDTVIAGDKKHLNGLSAISLIIVTYLMVVILVENIIGMSMP 242

Query: 246 TRFFTFSILLILLAAPLAIAINAQR-EDFRESSPSF-LTEKSHVVNKLKSMDAEDSVEYH 305
            +  +F+ LL+LLA+PL +A+ AQR E+ R  S  F +TE++ +++  K   + D  +  
Sbjct: 243 MKICSFTFLLLLLASPLLVAVRAQREEEHRFLSLDFPVTERTTLLDSPKLNSSSDVKDV- 302

Query: 306 ELPRDESQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSL 365
                           +   MNVLEAI T NFWLLF+AM+CGMGSGLATINN+ Q+G+SL
Sbjct: 303 ----------------MTNDMNVLEAICTTNFWLLFVAMICGMGSGLATINNIRQMGESL 362

Query: 366 GYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIAS 425
            Y+  +  + VSLWSIWNFLGRFG+GY SD  LHS+GW RP+ MAITL +M+ GHIV+AS
Sbjct: 363 RYSTVQLNSLVSLWSIWNFLGRFGSGYISDTYLHSHGWPRPVFMAITLGLMAIGHIVMAS 422

Query: 426 GFSGNLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVI 485
           G  G+LY+GS+L+G+ YGSQWSLMPTITSEIFG+ HMGTIF TI+IASP+GSY FSV+VI
Sbjct: 423 GLLGSLYIGSLLVGLAYGSQWSLMPTITSEIFGVLHMGTIFYTISIASPVGSYFFSVKVI 482

Query: 486 GYIYDREAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQRRV 540
           GY+YD+ A+ +  SC G HC   SFL+MA +A LGSLV   L  RT++FY   + +R+
Sbjct: 483 GYLYDKVASEDDHSCYGNHCFRTSFLIMAAMALLGSLVALVLLLRTKKFYATLVAKRI 523

BLAST of ClCG06G016480 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 556.2 bits (1432), Expect = 2.9e-158
Identity = 288/531 (54.24%), Postives = 377/531 (71.00%), Query Frame = 0

Query: 10  KWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGANAGIISGFLY 69
           KW+A A  IWIQ   GA+YTF+IYSS LKSSQ+YDQSTLD VSVFKDIG   GIISGFLY
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 70  SAVTPPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCFFMFLAAHAQ 129
           +A+T  +    F GPWVV   G +Q F+G+ FIWA++ G+I  PPVP MC F+FLA H+ 
Sbjct: 67  TAMTSKSR--GFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSL 126

Query: 130 TFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGD--PSNFLLMLAV 189
            FFNTANVVT   NF+ Y GT VGIM+G+LGLSGA+LIQ+Y+  C G+  P+ F+L+LA+
Sbjct: 127 PFFNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAI 186

Query: 190 LPTLLSIMFMWFVRIDKTKSSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSLSSWTRFF 249
           +PTL+  + M FVR+ +T + ++ KHL+ LSA+++I+A YLMVVI + N   LS   + F
Sbjct: 187 VPTLVMFLAMPFVRVYETVTISDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQIF 246

Query: 250 TFSILLILLAAPLAIAINAQREDFRESSPSFLTEKSHVVNKLKSMDAEDSVEYHELPRDE 309
           +F ++L+LLA+PL +A+ A RE  +                L S+D         L    
Sbjct: 247 SFILVLLLLASPLLVAVRALREKRQ---------------TLSSLDGPVLDTSALLDPPS 306

Query: 310 SQTVAVSNSRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATE 369
           S      +  + +  N+LEA+ T+NFWLLFLAM+CGMGSG AT+NNM Q+G+SL Y++ +
Sbjct: 307 SNIFPDGDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSVQ 366

Query: 370 TKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGGHIVIASGFSGNL 429
             + VSLWSIWNFLGRFGAGY SD  LH + W RP+ MAITL +M+ GHI++ASG  G+L
Sbjct: 367 LNSLVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGSL 426

Query: 430 YVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDR 489
           Y GS+LIG+ YGSQWSLMPTITSEIFG+ HMGTI+ TI+IA P+GSYI SV+VIGY YD+
Sbjct: 427 YAGSVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYDK 486

Query: 490 EAAGEHGSCSGTHCLLISFLVMAVVAFLGSLVVAALFFRTRRFYRLALQRR 539
            A+ +  SC G+ C   SF++MA VA  GSLV + LFFRT +FY+  + +R
Sbjct: 487 VASEDDNSCFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYKNLVAKR 520

BLAST of ClCG06G016480 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 342.0 bits (876), Expect = 8.5e-94
Identity = 200/555 (36.04%), Postives = 320/555 (57.66%), Query Frame = 0

Query: 7   MPNKWIATALGIWIQCICGASYTFSIYSSALKSSQAYDQSTLDIVSVFKDIGANAGIISG 66
           +  +W      + I    GA+Y F IYS  +K +  YDQ+TL+++S FKD+GAN G+++G
Sbjct: 18  LTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVLAG 77

Query: 67  FLYSAVTPPAPRWAFAGPWVVHAAGAIQCFLGYIFIWAAISGVIDRPPVPAMCFFMFLAA 126
            L + VTP         PW +   GAI  F GY  IW A++  I +P V  MC ++ + A
Sbjct: 78  LL-NEVTP---------PWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGA 137

Query: 127 HAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTTCNGDPSNFLLMLA 186
           ++Q+F NT ++VT V NF    G ++GI+KGY+GLSGA++ Q+Y      D    +LM+ 
Sbjct: 138 NSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIG 197

Query: 187 VLPTLLSIMFMWFVRIDKTK-SSNEMKHLNSLSALAVIVAFYLMVVIILNNTFSLSSWTR 246
            LP ++S  F+  +RI K K  +NE+K   +   +++ +A +LMVVII+N     +    
Sbjct: 198 WLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEF 257

Query: 247 FFTFSILLILLAAPLAIAINAQREDFRESSPSF--------LTEKSHV-VNKLKSMDAED 306
             + +++++LL  P+ + I  +++ ++E   +         +TEK  +  ++ K  D E+
Sbjct: 258 GGSAAVVIVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEE 317

Query: 307 SVEYHELPRDESQTVAVSN-SRIPQAMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMS 366
           S E  E  +  S    V N         +L+A+ +++  +LFLA +CG+G  L  I+N+ 
Sbjct: 318 SKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLG 377

Query: 367 QLGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLMIMSGG 426
           Q+G SLGY      TFVSL SIWN+ GR  +G  S+  L  Y + RPL++ + L++   G
Sbjct: 378 QIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAG 437

Query: 427 HIVIASGFSGNLYVGSILIGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYI 486
           H++IA    G LYV S++IG C+G+QW L+  I SEIFGL++  T++N  ++ASP+GSY+
Sbjct: 438 HLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYL 497

Query: 487 FSVRVIGYIYDREAA------------GEHGSCSGTHCLLISFLVMAVVAFLGSLVVAAL 539
            +VRV GY+YD EA             G+  +C GT C  +SF+++A V   G LV   L
Sbjct: 498 LNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVL 557

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879631.17.6e-27891.51protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida][more]
XP_008453166.11.7e-27790.59PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo] >KAA0057917.1 prote... [more]
XP_022972528.14.6e-27589.30protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima][more]
XP_023531919.13.9e-27489.11protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo][more]
KAG7021495.15.6e-27388.93Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
F4I9E11.0e-2725.90Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q6CGU82.2e-0927.18Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28... [more]
Q96TW92.4e-0827.57Probable transporter MCH1 OS=Wickerhamomyces anomalus OX=4927 GN=MCH1 PE=3 SV=1[more]
Q6FWD43.8e-0623.72Probable transporter MCH1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM ... [more]
Match NameE-valueIdentityDescription
A0A5A7UT758.2e-27890.59Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BWC28.2e-27890.59protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 ... [more]
A0A6J1IA872.2e-27589.30protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471... [more]
A0A6J1F6047.9e-27388.75protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A0A0LUE85.1e-27289.42Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G00847... [more]
Match NameE-valueIdentityDescription
AT1G74780.18.9e-17658.40Nodulin-like / Major Facilitator Superfamily protein [more]
AT1G18940.14.3e-16254.51Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34350.15.6e-16256.13Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34355.12.9e-15854.24Major facilitator superfamily protein [more]
AT2G39210.18.5e-9436.04Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 10..264
e-value: 3.1E-65
score: 220.2
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 211..525
e-value: 6.5E-12
score: 46.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 216..529
NoneNo IPR availablePANTHERPTHR21576:SF22F25A4.25 PROTEINcoord: 9..540
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 9..540
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 10..516
e-value: 5.34746E-115
score: 344.235

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG06G016480.1ClCG06G016480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity