ClCG06G005080 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG06G005080
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	chaperone protein dnaJ 49-like
Location	CG_Chr06: 5780013 .. 5781535 (+)
RNA-Seq Expression	ClCG06G005080
Synteny	ClCG06G005080

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCTTTTGTTTTTCTCAATGTTCTCCTCCGAAAGTCCCCAACGCATTTCATGGATATCAAAAGTCCTCTCTTGCTGACGAATGAACGCCATTGCAGCAGCCCGACTCCCAGCTCGGTGTTCATCGGTAAGAAATCTTTTGTACGAATTCGATCTTCAGAATGAATCAAGATCCTCATCTTTCTTCATGTTTCAAGCCATCAATCTCTTTATATCAGGTACGACTCTCATTTTTCCTCCTTTTCCATCTATCGTGTATATATGTTGTTGTTGTCGATGATGATGATGATGATGTTTACTCTAGCTTACGGTTGCGAAATTTGATAGTAGAATTCCCTTTTTCCTTAATTTAGGTATTCTCTATCTCGAGTTTGTGATCAGTCTAGTTGCAATTGAATTGTATCTCAAATTGGGCTGAAGGTAATTCGGGAGTTTAGGTTTTTTGGAAGATTGTGTCGGTGCGATTATGGATGGGAATAAGGACGAAGCTTTAAGATGTATTCGCATGGCGGAAGAAGCAATTGCATCGGGGAATAAAGAGCGAGCACTTAGATTCATTAAAATTGCTTGTCGCCTTAATCAGAGTTTGCAAGTGGATGAGCTATTGACGGCGTGCGAAGAGATCGGTTCGGGATCGCCTTCATCTTCTTCCGATGAGAAACGCGCGGGAAAAGTTGAGAGTGTTTCTGGTTCAGTGAAACATGTTGATGGTTTGAATGGTGAACGTAATTACACTATAGAACACGTTCAGTTGATTAGGCAGATAAAAACAACCAAGGACTATTACAGAATTCTTGGTGTGGAGAAAACTAGTTCGGCTGAAGAGATTAAGAAAGCTTATAAGAAATTGTCCTTGAAAGTTCACCCTGATAAAAACAAGGCTCCTGGTTCAGATGAAGCATTCAAGAAATTATCCAAGGCTTTCATGTGTTTGAGCGATGATACTTTGAGGAGGCAATATGATCACACGGGTTTGGTTGATCAGTATGAGTACAACCAACAGCATAATGTAAGGCATAGGAGAAGGAGAACGGGTCATGATTTGTTTGAAGAAAATTTCGATCCTGATGAGATACTCAGAGCGTTCTTTGGGCAGGGAAATATGTTTCAGACTAGTCGTGCTTATACTTATAGAACTGGAGGTGCGGGAAGCCAACAGAGGACAGAATCTGATGGGGGAGGTCCCAGCTTGTTGATTATTCTTCTAATGTTACCATTCTTGTTAATTTGCTTGTTGGCTTATATGCCATTTCCAGAGCCGGACTATGCTTTGCATAAAAACCTATCCTATAGTGTTCCTATGGCAACGGAGAAACATGGAGTGGAATTTTTTGTTAAATCATCTGATTTTGATGTAAGATATCCTCCTGGAAGCCCAGGTCGAGCTGAAATAGAGAATAGTGTGCTGAGGGATTATAGAAACATGGTTTGGCGTTATTGTCATGTAGAACTCCAGAGACGCCAGTGGAATAGAAATCTCCCAACTCCTCACTGCGAGAAGCTGAATAATCTCGGTGTAGCATAA

mRNA sequence

TTCTTTTGTTTTTCTCAATGTTCTCCTCCGAAAGTCCCCAACGCATTTCATGGATATCAAAAGTCCTCTCTTGCTGACGAATGAACGCCATTGCAGCAGCCCGACTCCCAGCTCGGTGTTCATCGGTAAGAAATCTTTTGTACGAATTCGATCTTCAGAATGAATCAAGATCCTCATCTTTCTTCATGTTTCAAGCCATCAATCTCTTTATATCAGGTTTTTTGGAAGATTGTGTCGGTGCGATTATGGATGGGAATAAGGACGAAGCTTTAAGATGTATTCGCATGGCGGAAGAAGCAATTGCATCGGGGAATAAAGAGCGAGCACTTAGATTCATTAAAATTGCTTGTCGCCTTAATCAGAGTTTGCAAGTGGATGAGCTATTGACGGCGTGCGAAGAGATCGGTTCGGGATCGCCTTCATCTTCTTCCGATGAGAAACGCGCGGGAAAAGTTGAGAGTGTTTCTGGTTCAGTGAAACATGTTGATGGTTTGAATGGTGAACGTAATTACACTATAGAACACGTTCAGTTGATTAGGCAGATAAAAACAACCAAGGACTATTACAGAATTCTTGGTGTGGAGAAAACTAGTTCGGCTGAAGAGATTAAGAAAGCTTATAAGAAATTGTCCTTGAAAGTTCACCCTGATAAAAACAAGGCTCCTGGTTCAGATGAAGCATTCAAGAAATTATCCAAGGCTTTCATGTGTTTGAGCGATGATACTTTGAGGAGGCAATATGATCACACGGGTTTGGTTGATCAGTATGAGTACAACCAACAGCATAATGTAAGGCATAGGAGAAGGAGAACGGGTCATGATTTGTTTGAAGAAAATTTCGATCCTGATGAGATACTCAGAGCGTTCTTTGGGCAGGGAAATATGTTTCAGACTAGTCGTGCTTATACTTATAGAACTGGAGGTGCGGGAAGCCAACAGAGGACAGAATCTGATGGGGGAGGTCCCAGCTTGTTGATTATTCTTCTAATGTTACCATTCTTGTTAATTTGCTTGTTGGCTTATATGCCATTTCCAGAGCCGGACTATGCTTTGCATAAAAACCTATCCTATAGTGTTCCTATGGCAACGGAGAAACATGGAGTGGAATTTTTTGTTAAATCATCTGATTTTGATGTAAGATATCCTCCTGGAAGCCCAGGTCGAGCTGAAATAGAGAATAGTGTGCTGAGGGATTATAGAAACATGGTTTGGCGTTATTGTCATGTAGAACTCCAGAGACGCCAGTGGAATAGAAATCTCCCAACTCCTCACTGCGAGAAGCTGAATAATCTCGGTGTAGCATAA

Coding sequence (CDS)

ATGAACGCCATTGCAGCAGCCCGACTCCCAGCTCGGTGTTCATCGGTAAGAAATCTTTTGTACGAATTCGATCTTCAGAATGAATCAAGATCCTCATCTTTCTTCATGTTTCAAGCCATCAATCTCTTTATATCAGGTTTTTTGGAAGATTGTGTCGGTGCGATTATGGATGGGAATAAGGACGAAGCTTTAAGATGTATTCGCATGGCGGAAGAAGCAATTGCATCGGGGAATAAAGAGCGAGCACTTAGATTCATTAAAATTGCTTGTCGCCTTAATCAGAGTTTGCAAGTGGATGAGCTATTGACGGCGTGCGAAGAGATCGGTTCGGGATCGCCTTCATCTTCTTCCGATGAGAAACGCGCGGGAAAAGTTGAGAGTGTTTCTGGTTCAGTGAAACATGTTGATGGTTTGAATGGTGAACGTAATTACACTATAGAACACGTTCAGTTGATTAGGCAGATAAAAACAACCAAGGACTATTACAGAATTCTTGGTGTGGAGAAAACTAGTTCGGCTGAAGAGATTAAGAAAGCTTATAAGAAATTGTCCTTGAAAGTTCACCCTGATAAAAACAAGGCTCCTGGTTCAGATGAAGCATTCAAGAAATTATCCAAGGCTTTCATGTGTTTGAGCGATGATACTTTGAGGAGGCAATATGATCACACGGGTTTGGTTGATCAGTATGAGTACAACCAACAGCATAATGTAAGGCATAGGAGAAGGAGAACGGGTCATGATTTGTTTGAAGAAAATTTCGATCCTGATGAGATACTCAGAGCGTTCTTTGGGCAGGGAAATATGTTTCAGACTAGTCGTGCTTATACTTATAGAACTGGAGGTGCGGGAAGCCAACAGAGGACAGAATCTGATGGGGGAGGTCCCAGCTTGTTGATTATTCTTCTAATGTTACCATTCTTGTTAATTTGCTTGTTGGCTTATATGCCATTTCCAGAGCCGGACTATGCTTTGCATAAAAACCTATCCTATAGTGTTCCTATGGCAACGGAGAAACATGGAGTGGAATTTTTTGTTAAATCATCTGATTTTGATGTAAGATATCCTCCTGGAAGCCCAGGTCGAGCTGAAATAGAGAATAGTGTGCTGAGGGATTATAGAAACATGGTTTGGCGTTATTGTCATGTAGAACTCCAGAGACGCCAGTGGAATAGAAATCTCCCAACTCCTCACTGCGAGAAGCTGAATAATCTCGGTGTAGCATAA

Protein sequence

MNAIAAARLPARCSSVRNLLYEFDLQNESRSSSFFMFQAINLFISGFLEDCVGAIMDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSSSSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVEKTSSAEEIKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQHNVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGPSLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATEKHGVEFFVKSSDFDVRYPPGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGVA

Homology

[Close]

BLAST of ClCG06G005080 vs. NCBI nr
Match: XP_038874732.1 (chaperone protein dnaJ 49 [Benincasa hispida] >XP_038874733.1 chaperone protein dnaJ 49 [Benincasa hispida] >XP_038874734.1 chaperone protein dnaJ 49 [Benincasa hispida])

HSP 1 Score: 669.5 bits (1726), Expect = 1.9e-188
Identity = 333/352 (94.60%), Postives = 342/352 (97.16%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           MDGNKDEALRCIR+AEEAIASGNKERALRFIKIA RLNQSLQVDELLT CEEIGSGSPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALRFIKIARRLNQSLQVDELLTVCEEIGSGSPSS 60

Query: 116 SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVEKTSSAEE 175
           S DEKRAGKVES SGSVKHVDGL+GERNY+IEHVQLIRQIKTT+DYYRILGVEKTSSAEE
Sbjct: 61  SFDEKRAGKVESASGSVKHVDGLSGERNYSIEHVQLIRQIKTTEDYYRILGVEKTSSAEE 120

Query: 176 IKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 235
           IK+AYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLV QYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVAQYEYNQQH 180

Query: 236 NVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 295
           NVRHRRRRTGHDLFEENFDPDEI RAFFGQGNMFQTSR+YTYRTGGA SQQRTES GGGP
Sbjct: 181 NVRHRRRRTGHDLFEENFDPDEIFRAFFGQGNMFQTSRSYTYRTGGARSQQRTESAGGGP 240

Query: 296 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATEKHGVEFFVKSSDFDVRYP 355
           SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYS+P+ TEKHGVEFFVKSSDFDVRYP
Sbjct: 241 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSIPVITEKHGVEFFVKSSDFDVRYP 300

Query: 356 PGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGVA 408
            GSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWN+NLPTPHCEKLNNLGVA
Sbjct: 301 LGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNKNLPTPHCEKLNNLGVA 352

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BLAST of ClCG06G005080 vs. NCBI nr
Match: XP_016902270.1 (PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >XP_016902271.1 PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >XP_016902272.1 PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo])

HSP 1 Score: 639.8 bits (1649), Expect = 1.6e-179
Identity = 318/352 (90.34%), Postives = 333/352 (94.60%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           MDGNKDEALRCIR+AEE+IASGNKERALRFIKIA RLNQSLQ+DELLTACEE+GSG    
Sbjct: 1   MDGNKDEALRCIRIAEESIASGNKERALRFIKIARRLNQSLQIDELLTACEEMGSG---- 60

Query: 116 SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVEKTSSAEE 175
           SS EKRAGK ESVSGSVKHVDGLNGERNY++EHVQLIRQIKTT DYY ILGVEKTSSAEE
Sbjct: 61  SSAEKRAGKGESVSGSVKHVDGLNGERNYSMEHVQLIRQIKTTMDYYGILGVEKTSSAEE 120

Query: 176 IKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 235
           IK+AY+KLSLKVHPDKNKAPGS+EAFKKLSKAF CLSDDTLRR+YD T LVDQYEYNQQH
Sbjct: 121 IKRAYRKLSLKVHPDKNKAPGSEEAFKKLSKAFSCLSDDTLRRRYDQTALVDQYEYNQQH 180

Query: 236 NVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 295
           NVRHRRRR GHDLFEENFDPDEI RAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP
Sbjct: 181 NVRHRRRRNGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 240

Query: 296 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATEKHGVEFFVKSSDFDVRYP 355
           S LIILLMLPFLLICLLAYMPFPEP+YALHKNLSYS+PMATEKHGVEFFVKSSDF+VRYP
Sbjct: 241 SFLIILLMLPFLLICLLAYMPFPEPEYALHKNLSYSIPMATEKHGVEFFVKSSDFNVRYP 300

Query: 356 PGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGVA 408
            GSPGR EIENSVLRDYRNMVWRYCH+ELQRRQWN+NLPTPHCEKLN L VA
Sbjct: 301 LGSPGRVEIENSVLRDYRNMVWRYCHIELQRRQWNKNLPTPHCEKLNTLAVA 348

[Close]

BLAST of ClCG06G005080 vs. NCBI nr
Match: XP_004148209.1 (chaperone protein dnaJ 49 [Cucumis sativus] >XP_011657260.1 chaperone protein dnaJ 49 [Cucumis sativus] >XP_011657262.1 chaperone protein dnaJ 49 [Cucumis sativus] >KGN47372.1 hypothetical protein Csa_022981 [Cucumis sativus])

HSP 1 Score: 632.5 bits (1630), Expect = 2.5e-177
Identity = 315/352 (89.49%), Postives = 331/352 (94.03%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           MDGNKDEALRCIR+AEE+IASGNKERALRFIKIA RLNQS+QVDELL ACEEIGSG    
Sbjct: 1   MDGNKDEALRCIRIAEESIASGNKERALRFIKIARRLNQSVQVDELLAACEEIGSG---- 60

Query: 116 SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVEKTSSAEE 175
           SS+EKRAGK ESVSGSVKH DGLNGERNY++EHVQLIRQIKTTKDYY ILGVEKTSSAEE
Sbjct: 61  SSEEKRAGKGESVSGSVKHGDGLNGERNYSMEHVQLIRQIKTTKDYYGILGVEKTSSAEE 120

Query: 176 IKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 235
           IK+AY+KLSLKVHPDKNKAPGS+EAFKKLSKAF CLSDDTLRRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYRKLSLKVHPDKNKAPGSEEAFKKLSKAFSCLSDDTLRRQYDHTPLVDQYEYNQQH 180

Query: 236 NVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 295
           NVR RRRR GHDLFEENFDPDEI RAFFGQGNMFQTSRAYTYRTGGAGSQQRTES GGGP
Sbjct: 181 NVRQRRRRNGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESYGGGP 240

Query: 296 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATEKHGVEFFVKSSDFDVRYP 355
           + LIILLMLPFLLICLLAYMPFPEP+YALHK+LSYS+PMATEKHGVEFFVKSSDFD RYP
Sbjct: 241 NFLIILLMLPFLLICLLAYMPFPEPEYALHKSLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 356 PGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGVA 408
            GSPGR E+ENSVLRDYRNMVWRYCH+ELQRRQWN+NLPTPHCEKLN L VA
Sbjct: 301 LGSPGRVELENSVLRDYRNMVWRYCHIELQRRQWNKNLPTPHCEKLNTLAVA 348

[Close]

BLAST of ClCG06G005080 vs. NCBI nr
Match: KAG6575070.1 (Chaperone protein dnaJ 49, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 621.3 bits (1601), Expect = 5.9e-174
Identity = 309/352 (87.78%), Postives = 329/352 (93.47%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           MDGNKDEALRCIR+AEEAIASGNKERAL+FIKIACRLN+SL VDELL+ACEEI   SPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLGVDELLSACEEIDLKSPSS 60

Query: 116 SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVEKTSSAEE 175
           SSD KRAGKV SVSGS  HVDGLNGERNY+IEHVQLIRQIKT KDYY+ILGVE+TSSAEE
Sbjct: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVERTSSAEE 120

Query: 176 IKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 235
           IK+AYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDT RRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180

Query: 236 NVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 295
           NV  RRRRTG DLFEENFDPDEI RAFFGQGNMFQTSRAYTY TGGA SQ+RTESDGGGP
Sbjct: 181 NV--RRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGP 240

Query: 296 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATEKHGVEFFVKSSDFDVRYP 355
           +LLI+LLMLPFLLI LLAYMPFPEPDY+LHKNLSYS+PMATEKHGVEFFVKSSDFD RYP
Sbjct: 241 NLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 356 PGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGVA 408
            GS  R+EIE+SV+RDY+NMVWRYCHVELQRR+WN+NLPTPHCEK+NNLG A
Sbjct: 301 LGSGARSEIESSVIRDYKNMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350

[Close]

BLAST of ClCG06G005080 vs. NCBI nr
Match: XP_022958924.1 (chaperone protein dnaJ 49 [Cucurbita moschata] >XP_022958925.1 chaperone protein dnaJ 49 [Cucurbita moschata])

HSP 1 Score: 620.9 bits (1600), Expect = 7.6e-174
Identity = 307/352 (87.22%), Postives = 331/352 (94.03%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           MDGNKDEALRCIR+AEEAIASGNKERAL+FIKIACRLN+SL+V+ELL+ACE+I S SPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLEVNELLSACEDIDSKSPSS 60

Query: 116 SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVEKTSSAEE 175
           SSD KRAGKV SVSGS  HVDGLNGERNY+IEHVQLIRQIKT KDYY+ILGVEKTSSAEE
Sbjct: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVEKTSSAEE 120

Query: 176 IKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 235
           IK+AYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLS+DT RRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSNDTSRRQYDHTALVDQYEYNQQH 180

Query: 236 NVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 295
           NV  RRRRTG DLFEENFDPDEI RAFFGQGNMFQTSRAYTY TGGA SQ+RTESDGGGP
Sbjct: 181 NV--RRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGP 240

Query: 296 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATEKHGVEFFVKSSDFDVRYP 355
           +LLI+LLMLPFLLI LLAYMPFPEPDY+LHKNLSYS+PMATEKHGVEFFVKSSDFD RYP
Sbjct: 241 NLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 356 PGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGVA 408
            GS  R+EIE+SV+RDY+NMVWRYCH+ELQRR+WN+NLPTPHCEK+NNLG A
Sbjct: 301 LGSGARSEIESSVIRDYKNMVWRYCHIELQRRKWNKNLPTPHCEKMNNLGFA 350

[Close]

BLAST of ClCG06G005080 vs. ExPASy Swiss-Prot
Match: Q9FH28 (Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2)

HSP 1 Score: 340.1 bits (871), Expect = 3.4e-92
Identity = 181/354 (51.13%), Postives = 251/354 (70.90%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           MDGNKD+A RC+R+AE+AI SG+KERAL+FI +A RLN SL VDEL+ AC+ + S S +S
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSLSVDELVAACDNLDSVSRNS 60

Query: 116 SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVEKTSSAEE 175
           S  E    K++++ G    ++   G+  YT E+V L+R I    DYY ILG+EK  S +E
Sbjct: 61  SVSE----KLKTMDGDDDKLE--TGKMKYTEENVDLVRNIIRNNDYYAILGLEKNCSVDE 120

Query: 176 IKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 235
           I+KAY+KLSLKVHPDKNKAPGS+EAFKK+SKAF CLSD   RRQ+D  G+VD++++ Q+ 
Sbjct: 121 IRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFDQVGIVDEFDHVQRR 180

Query: 236 NVRHRRR-RTGHDLFEENFDPDEILRAFFG-QGNMFQTSRAYTYRTGGAGSQQRTES-DG 295
           N R RRR  T +D F++ FDP+EI R  FG Q  +F+ S A  YRT    +Q R E  + 
Sbjct: 181 NRRPRRRYNTRNDFFDDEFDPEEIFRTVFGQQREVFRASHA--YRTRQPRNQFREEEINV 240

Query: 296 GGPSLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATEKHGVEFFVKS-SDFD 355
            GPS L I+ +LPF L+ LLAY+PF EPDY+LHKN SY +P  T+   + F+V+S S FD
Sbjct: 241 AGPSCLTIIQILPFFLLLLLAYLPFSEPDYSLHKNQSYQIPKTTQNTEISFYVRSASAFD 300

Query: 356 VRYPPGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLG 406
            ++P  S  RA +E +V+++Y++ +++ C +ELQ+R+WN+ +PTPHC +L + G
Sbjct: 301 EKFPLSSSARANLEGNVIKEYKHFLFQSCRIELQKRRWNKKIPTPHCIELQDRG 346

[Close]

BLAST of ClCG06G005080 vs. ExPASy Swiss-Prot
Match: Q7ZXQ8 (DnaJ homolog subfamily B member 14 OS=Xenopus laevis OX=8355 GN=dnajb14 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 4.1e-37
Identity = 113/348 (32.47%), Postives = 178/348 (51.15%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           M+ N+DEA RC+R+ + AI +G+KE+A RF   A RL  S +   LL A E+  + +  +
Sbjct: 1   MESNRDEAERCVRIGKAAIEAGDKEKARRFFSKAERLYPSSEARVLLDALEK--NDTAGN 60

Query: 116 SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVEKTSSAEE 175
               ++  K      + K   G  G + +T + V  +++IK  K YY +LGV   +  E+
Sbjct: 61  GPQSEKMSKSTEQPKAEKDSSGDTG-KGHTQDQVDGVQRIKKCKTYYEVLGVSPDAGEED 120

Query: 176 IKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 235
           +KKAY+KL+LK HPDKN APG+ EAFKK+  A+  LS+   R+QYD TG  D        
Sbjct: 121 LKKAYRKLALKFHPDKNHAPGATEAFKKIGNAYAVLSNPEKRKQYDLTGSED-------- 180

Query: 236 NVRHRRRRTGHDL---FEENFDPDEILRAFFGQGNMFQTSRAYTYRTG-----------G 295
           NV++  R  G D    FE +  P+++   FFG G  F +   +T+  G            
Sbjct: 181 NVQNNHRNGGFDYHRGFEADITPEDLFNMFFGGG--FPSGSVHTFSNGRTRYSHHQHHHH 240

Query: 296 AGSQQRTESDGGGPSLLIILL-MLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATEKHG 355
           +G  +  E   GG S+ I L+ ++  +L+ LL+ +    P Y+L+     ++   TE   
Sbjct: 241 SGHDREEERADGGFSMFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGQTIKRVTENLQ 300

Query: 356 VEFFVKSSDFDVRYPPGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQ 389
           + ++V S DF   Y        ++E ++  DY   V   C  E Q++Q
Sbjct: 301 ISYYV-SKDFKSEY--NGMLLQKLEKNIEEDYVANVRNNCWRERQQKQ 332

[Close]

BLAST of ClCG06G005080 vs. ExPASy Swiss-Prot
Match: Q28I38 (DnaJ homolog subfamily B member 14 OS=Xenopus tropicalis OX=8364 GN=dnajb14 PE=2 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 5.4e-37
Identity = 116/347 (33.43%), Postives = 178/347 (51.30%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEE---IGSGS 115
           M+ N+DEA RC+R+A+ AI +G+KE+A RF+  A RL  S +   LL A E+    G+G 
Sbjct: 1   MESNRDEAERCVRIAKAAIEAGDKEKAKRFLSKAERLYPSSEARALLQAFEKNDTAGNGP 60

Query: 116 PSS----SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVE 175
            S+     +++ +A K  + S S       +  + +T + +  +++IK  K YY +LGV 
Sbjct: 61  QSAKMAKGTEQPKAEKDSNASAS------SDTGKGHTQDQLDGVQRIKKCKTYYEVLGVS 120

Query: 176 KTSSAEEIKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQ 235
             +  E++KKAY+KL+LK HPDKN APG+ EAFKK+  A+  LS+   R+QYD TG  DQ
Sbjct: 121 TDAGEEDLKKAYRKLALKFHPDKNHAPGATEAFKKIGNAYAVLSNPEKRKQYDLTGSEDQ 180

Query: 236 YEYNQQHNVRHRRRRTGHDL---FEENFDPDEILRAFFGQGNMFQTSRAYTYRTGGA--- 295
            + N         R  G D    FE +  P+++   FFG G  F +   +T+  G A   
Sbjct: 181 MQNN--------HRNGGFDYHRGFEADITPEDLFNMFFGGG--FPSGSVHTFSNGRARYS 240

Query: 296 --------GSQQRTESDGGGPSLLIILL-MLPFLLICLLAYMPFPEPDYALHKNLSYSVP 355
                   G  +  E   GG S+ I L+ ++  +L+ LL+      P Y+L+     +  
Sbjct: 241 HHQHHHHSGHDREDERADGGFSMFIQLMPIIVLILVSLLSQFMVSNPPYSLYPRSGQATK 300

Query: 356 MATEKHGVEFFVKSSDFDVRYPPGSPGRAE--IENSVLRDYRNMVWR 379
             TE   + ++V S DF   Y      + E  IE   + + RN  WR
Sbjct: 301 RVTENLQIAYYV-SKDFQSEYSGILLQKLEKNIEEDYVANVRNNCWR 330

[Close]

BLAST of ClCG06G005080 vs. ExPASy Swiss-Prot
Match: Q149L6 (DnaJ homolog subfamily B member 14 OS=Mus musculus OX=10090 GN=Dnajb14 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 3.5e-36
Identity = 116/348 (33.33%), Postives = 185/348 (53.16%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           M+GN+DEA +C+++A EA+++GN+++A RF++ A +L        LL    + GS + SS
Sbjct: 1   MEGNRDEAEKCVQIAREALSAGNRDKAQRFLQKAEKLYPLPAARALLEIIMKNGSTAGSS 60

Query: 116 SSDEKRAGKVESVSGSVKHVDGLNGE----RNYTIEHVQLIRQIKTTKDYYRILGVEKTS 175
           +   K  G  +    S    DG +G     + YT + V+ +  I   K+YY +LGV K +
Sbjct: 61  THCRKPPGSSDQSKPSCGK-DGTSGAGEGGKVYTKDQVEGVLSINKCKNYYEVLGVTKDA 120

Query: 176 SAEEIKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEY 235
             E++KKAY+KL+LK HPDKN APG+ +AFKK+  A+  LS+   R+QYD TG  +Q   
Sbjct: 121 GDEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQYDLTGSEEQ-AC 180

Query: 236 NQQHNVRHRRRRTGHDLFEENFDPDEILRAFFGQG------NMFQTSRA---YTYRTGGA 295
           N Q+N R    R      E +  P+++   FFG G      + F   RA   + ++   +
Sbjct: 181 NHQNNGRFNFHRG----CEADITPEDLFNIFFGGGFPSGSVHSFSNGRAAYSHQHQHRHS 240

Query: 296 GSQQRTESDGGGPSLLIILL-MLPFLLICLLAYMPFPEPDYALH--KNLSYSVPMATEKH 355
           G ++  E   GG S+ I L+ ++  +L+ LL+ +    P Y+L+       ++ M TE  
Sbjct: 241 GHEREEERADGGFSVFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGSGQTIKMQTENL 300

Query: 356 GVEFFVKSSDFDVRYPPGSPGRAEIENSVLRDYRNMVWRYCHVELQRR 388
           GV ++V S DF   Y        ++E SV  DY   +   C  E Q++
Sbjct: 301 GVVYYV-SKDFKSEYK--GTLLQKVEKSVEEDYVTNIRNNCWKERQQK 339

[Close]

BLAST of ClCG06G005080 vs. ExPASy Swiss-Prot
Match: Q8TBM8 (DnaJ homolog subfamily B member 14 OS=Homo sapiens OX=9606 GN=DNAJB14 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 4.5e-36
Identity = 115/355 (32.39%), Postives = 182/355 (51.27%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGS--- 115
           M+GN+DEA +C+ +A EA+ +GN+E+A RF++ A +L        LL    + GS +   
Sbjct: 1   MEGNRDEAEKCVEIAREALNAGNREKAQRFLQKAEKLYPLPSARALLEIIMKNGSTAGNS 60

Query: 116 -----PSSSSDEKRAG-KVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILG 175
                PS S D+ +     +S SGS +      G + YT + V  +  I   K+YY +LG
Sbjct: 61  PHCRKPSGSGDQSKPNCTKDSTSGSGE------GGKGYTKDQVDGVLSINKCKNYYEVLG 120

Query: 176 VEKTSSAEEIKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLV 235
           V K +  E++KKAY+KL+LK HPDKN APG+ +AFKK+  A+  LS+   R+QYD TG  
Sbjct: 121 VTKDAGDEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQYDLTGNE 180

Query: 236 DQYEYNQQHNVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMFQTSRAYTYRTGGAGSQQ 295
           +Q   N Q+N R    R      E +  P+++   FFG G  F +   +++  G AG  Q
Sbjct: 181 EQ-ACNHQNNGRFNFHRG----CEADITPEDLFNIFFGGG--FPSGSVHSFSNGRAGYSQ 240

Query: 296 RTE------------SDGGGPSLLIILLMLPFLLICLLAYMPFPEPDYALH--KNLSYSV 355
           + +             DGG    + ++ ++  +L+ LL+ +    P Y+L+       ++
Sbjct: 241 QHQHRHSGHEREEERGDGGFSVFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGTGQTI 300

Query: 356 PMATEKHGVEFFVKSSDFDVRYPPGSPGRAEIENSVLRDYRNMVWRYCHVELQRR 388
            M TE  GV ++V + DF   Y        ++E SV  DY   +   C  E Q++
Sbjct: 301 KMQTENLGVVYYV-NKDFKNEYK--GMLLQKVEKSVEEDYVTNIRNNCWKERQQK 339

[Close]

BLAST of ClCG06G005080 vs. ExPASy TrEMBL
Match: A0A1S4E2R1 (chaperone protein dnaJ 49-like OS=Cucumis melo OX=3656 GN=LOC103497850 PE=4 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 7.7e-180
Identity = 318/352 (90.34%), Postives = 333/352 (94.60%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           MDGNKDEALRCIR+AEE+IASGNKERALRFIKIA RLNQSLQ+DELLTACEE+GSG    
Sbjct: 1   MDGNKDEALRCIRIAEESIASGNKERALRFIKIARRLNQSLQIDELLTACEEMGSG---- 60

Query: 116 SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVEKTSSAEE 175
           SS EKRAGK ESVSGSVKHVDGLNGERNY++EHVQLIRQIKTT DYY ILGVEKTSSAEE
Sbjct: 61  SSAEKRAGKGESVSGSVKHVDGLNGERNYSMEHVQLIRQIKTTMDYYGILGVEKTSSAEE 120

Query: 176 IKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 235
           IK+AY+KLSLKVHPDKNKAPGS+EAFKKLSKAF CLSDDTLRR+YD T LVDQYEYNQQH
Sbjct: 121 IKRAYRKLSLKVHPDKNKAPGSEEAFKKLSKAFSCLSDDTLRRRYDQTALVDQYEYNQQH 180

Query: 236 NVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 295
           NVRHRRRR GHDLFEENFDPDEI RAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP
Sbjct: 181 NVRHRRRRNGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 240

Query: 296 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATEKHGVEFFVKSSDFDVRYP 355
           S LIILLMLPFLLICLLAYMPFPEP+YALHKNLSYS+PMATEKHGVEFFVKSSDF+VRYP
Sbjct: 241 SFLIILLMLPFLLICLLAYMPFPEPEYALHKNLSYSIPMATEKHGVEFFVKSSDFNVRYP 300

Query: 356 PGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGVA 408
            GSPGR EIENSVLRDYRNMVWRYCH+ELQRRQWN+NLPTPHCEKLN L VA
Sbjct: 301 LGSPGRVEIENSVLRDYRNMVWRYCHIELQRRQWNKNLPTPHCEKLNTLAVA 348

[Close]

BLAST of ClCG06G005080 vs. ExPASy TrEMBL
Match: A0A0A0KG46 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G306340 PE=4 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 1.2e-177
Identity = 315/352 (89.49%), Postives = 331/352 (94.03%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           MDGNKDEALRCIR+AEE+IASGNKERALRFIKIA RLNQS+QVDELL ACEEIGSG    
Sbjct: 1   MDGNKDEALRCIRIAEESIASGNKERALRFIKIARRLNQSVQVDELLAACEEIGSG---- 60

Query: 116 SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVEKTSSAEE 175
           SS+EKRAGK ESVSGSVKH DGLNGERNY++EHVQLIRQIKTTKDYY ILGVEKTSSAEE
Sbjct: 61  SSEEKRAGKGESVSGSVKHGDGLNGERNYSMEHVQLIRQIKTTKDYYGILGVEKTSSAEE 120

Query: 176 IKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 235
           IK+AY+KLSLKVHPDKNKAPGS+EAFKKLSKAF CLSDDTLRRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYRKLSLKVHPDKNKAPGSEEAFKKLSKAFSCLSDDTLRRQYDHTPLVDQYEYNQQH 180

Query: 236 NVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 295
           NVR RRRR GHDLFEENFDPDEI RAFFGQGNMFQTSRAYTYRTGGAGSQQRTES GGGP
Sbjct: 181 NVRQRRRRNGHDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESYGGGP 240

Query: 296 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATEKHGVEFFVKSSDFDVRYP 355
           + LIILLMLPFLLICLLAYMPFPEP+YALHK+LSYS+PMATEKHGVEFFVKSSDFD RYP
Sbjct: 241 NFLIILLMLPFLLICLLAYMPFPEPEYALHKSLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 356 PGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGVA 408
            GSPGR E+ENSVLRDYRNMVWRYCH+ELQRRQWN+NLPTPHCEKLN L VA
Sbjct: 301 LGSPGRVELENSVLRDYRNMVWRYCHIELQRRQWNKNLPTPHCEKLNTLAVA 348

[Close]

BLAST of ClCG06G005080 vs. ExPASy TrEMBL
Match: A0A6J1H4U8 (chaperone protein dnaJ 49 OS=Cucurbita moschata OX=3662 GN=LOC111460072 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.7e-174
Identity = 307/352 (87.22%), Postives = 331/352 (94.03%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           MDGNKDEALRCIR+AEEAIASGNKERAL+FIKIACRLN+SL+V+ELL+ACE+I S SPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLEVNELLSACEDIDSKSPSS 60

Query: 116 SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVEKTSSAEE 175
           SSD KRAGKV SVSGS  HVDGLNGERNY+IEHVQLIRQIKT KDYY+ILGVEKTSSAEE
Sbjct: 61  SSDGKRAGKVHSVSGSANHVDGLNGERNYSIEHVQLIRQIKTAKDYYKILGVEKTSSAEE 120

Query: 176 IKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 235
           IK+AYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLS+DT RRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSNDTSRRQYDHTALVDQYEYNQQH 180

Query: 236 NVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 295
           NV  RRRRTG DLFEENFDPDEI RAFFGQGNMFQTSRAYTY TGGA SQ+RTESDGGGP
Sbjct: 181 NV--RRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGP 240

Query: 296 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATEKHGVEFFVKSSDFDVRYP 355
           +LLI+LLMLPFLLI LLAYMPFPEPDY+LHKNLSYS+PMATEKHGVEFFVKSSDFD RYP
Sbjct: 241 NLLILLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYSIPMATEKHGVEFFVKSSDFDERYP 300

Query: 356 PGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGVA 408
            GS  R+EIE+SV+RDY+NMVWRYCH+ELQRR+WN+NLPTPHCEK+NNLG A
Sbjct: 301 LGSGARSEIESSVIRDYKNMVWRYCHIELQRRKWNKNLPTPHCEKMNNLGFA 350

[Close]

BLAST of ClCG06G005080 vs. ExPASy TrEMBL
Match: A0A6J1KXF3 (chaperone protein dnaJ 49 OS=Cucurbita maxima OX=3661 GN=LOC111499080 PE=4 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 4.1e-173
Identity = 306/352 (86.93%), Postives = 329/352 (93.47%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           MDGNKDEALRCIR+AEEAIASGNKERAL+FIKIACRLN+SL+VDELL+ACEEI S SPSS
Sbjct: 1   MDGNKDEALRCIRIAEEAIASGNKERALKFIKIACRLNRSLEVDELLSACEEIDSKSPSS 60

Query: 116 SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVEKTSSAEE 175
           SSD KR GKV SVSGS  HVDGLNGERNY+IE+VQLIRQIKT KDYY+ILGVEKTSSAEE
Sbjct: 61  SSDGKRTGKVHSVSGSANHVDGLNGERNYSIENVQLIRQIKTAKDYYKILGVEKTSSAEE 120

Query: 176 IKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 235
           IK+AYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDT RRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTALVDQYEYNQQH 180

Query: 236 NVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMFQTSRAYTYRTGGAGSQQRTESDGGGP 295
           NV  RRRRTG DLFEENFDPDEI RAFFGQGNMFQTSRAYTY TGGA SQ+RTESDGGGP
Sbjct: 181 NV--RRRRTGRDLFEENFDPDEIFRAFFGQGNMFQTSRAYTYSTGGARSQRRTESDGGGP 240

Query: 296 SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATEKHGVEFFVKSSDFDVRYP 355
           +LL++LLMLPFLLI LLAYMPFPEPDY+LHKNLSY +PMATEKHGVEFFVKSSDFD RYP
Sbjct: 241 NLLVLLLMLPFLLIVLLAYMPFPEPDYSLHKNLSYIIPMATEKHGVEFFVKSSDFDERYP 300

Query: 356 PGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLGVA 408
            GS  R+EIE+SV+RDY++MVWRYCHVELQRR+WN+NLPTPHCEK+NNLG A
Sbjct: 301 LGSRARSEIESSVIRDYKSMVWRYCHVELQRRKWNKNLPTPHCEKMNNLGFA 350

[Close]

BLAST of ClCG06G005080 vs. ExPASy TrEMBL
Match: A0A6J1CB72 (chaperone protein dnaJ 49 OS=Momordica charantia OX=3673 GN=LOC111009903 PE=4 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 5.9e-172
Identity = 302/348 (86.78%), Postives = 326/348 (93.68%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           MDGNKDEALRCI +AE AIASGNK RALRFIKIA RLN+SLQVDELL ACEE+ SGSP+S
Sbjct: 1   MDGNKDEALRCIHIAEAAIASGNKARALRFIKIARRLNRSLQVDELLKACEEVDSGSPTS 60

Query: 116 SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVEKTSSAEE 175
           SSDEKRAGKVESV GS KHV+GLNGERNYT EHV+LIRQI TTKDYYRILGVEKTSSAEE
Sbjct: 61  SSDEKRAGKVESVPGSAKHVEGLNGERNYTTEHVRLIRQINTTKDYYRILGVEKTSSAEE 120

Query: 176 IKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 235
           IK+AYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDT RRQYDHT LVDQYEYNQQH
Sbjct: 121 IKRAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTSRRQYDHTDLVDQYEYNQQH 180

Query: 236 NVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMF-QTSRAYTYRTGGAGSQQRTESDGGG 295
           NVR RRRRTGHDLFEENFDP+EI RAFFGQGNMF  +SRAYTYRTGG GSQQR ES+GGG
Sbjct: 181 NVRQRRRRTGHDLFEENFDPEEIFRAFFGQGNMFHSSSRAYTYRTGGGGSQQRAESNGGG 240

Query: 296 PSLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATEKHGVEFFVKSSDFDVRY 355
           P+LLI+LLMLPFLLI LLAY+PFPEPDY+LH NLSY++PMATEKHGVEF+VKSSDFDV+Y
Sbjct: 241 PNLLILLLMLPFLLIFLLAYLPFPEPDYSLHTNLSYTIPMATEKHGVEFYVKSSDFDVKY 300

Query: 356 PPGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLN 403
           PPGS  RAEIEN+VLRDY++MVWRYCH+ELQRRQWN+NLPTPHCEK+N
Sbjct: 301 PPGSLARAEIENTVLRDYKSMVWRYCHIELQRRQWNKNLPTPHCEKMN 348

[Close]

BLAST of ClCG06G005080 vs. TAIR 10
Match: AT5G49060.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 340.1 bits (871), Expect = 2.4e-93
Identity = 181/354 (51.13%), Postives = 251/354 (70.90%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           MDGNKD+A RC+R+AE+AI SG+KERAL+FI +A RLN SL VDEL+ AC+ + S S +S
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSLSVDELVAACDNLDSVSRNS 60

Query: 116 SSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGVEKTSSAEE 175
           S  E    K++++ G    ++   G+  YT E+V L+R I    DYY ILG+EK  S +E
Sbjct: 61  SVSE----KLKTMDGDDDKLE--TGKMKYTEENVDLVRNIIRNNDYYAILGLEKNCSVDE 120

Query: 176 IKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVDQYEYNQQH 235
           I+KAY+KLSLKVHPDKNKAPGS+EAFKK+SKAF CLSD   RRQ+D  G+VD++++ Q+ 
Sbjct: 121 IRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFDQVGIVDEFDHVQRR 180

Query: 236 NVRHRRR-RTGHDLFEENFDPDEILRAFFG-QGNMFQTSRAYTYRTGGAGSQQRTES-DG 295
           N R RRR  T +D F++ FDP+EI R  FG Q  +F+ S A  YRT    +Q R E  + 
Sbjct: 181 NRRPRRRYNTRNDFFDDEFDPEEIFRTVFGQQREVFRASHA--YRTRQPRNQFREEEINV 240

Query: 296 GGPSLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATEKHGVEFFVKS-SDFD 355
            GPS L I+ +LPF L+ LLAY+PF EPDY+LHKN SY +P  T+   + F+V+S S FD
Sbjct: 241 AGPSCLTIIQILPFFLLLLLAYLPFSEPDYSLHKNQSYQIPKTTQNTEISFYVRSASAFD 300

Query: 356 VRYPPGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPHCEKLNNLG 406
            ++P  S  RA +E +V+++Y++ +++ C +ELQ+R+WN+ +PTPHC +L + G
Sbjct: 301 EKFPLSSSARANLEGNVIKEYKHFLFQSCRIELQKRRWNKKIPTPHCIELQDRG 346

[Close]

BLAST of ClCG06G005080 vs. TAIR 10
Match: AT3G57340.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 240.0 bits (611), Expect = 3.4e-63
Identity = 140/370 (37.84%), Postives = 216/370 (58.38%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           MDGNKD+AL+C+++ + A+ +G++ RAL+F+  A RL+ +L +D+L++      S  P S
Sbjct: 1   MDGNKDDALKCLKICKSAMEAGDRPRALKFLAKARRLDPNLPIDDLVSELNNNKSDEPGS 60

Query: 116 SSDEKRAGKVESVSGSVK--------HVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGV 175
           +     A   +S + S +             +   +YT E + ++R+IK+ KDYY ILG+
Sbjct: 61  AKSPGSAAAKDSSNSSDRPSLRQRGSSTTSSSSSMSYTEEQISIVRKIKSKKDYYEILGL 120

Query: 176 EKTSSAEEIKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVD 235
           E   S ++++KAY+KLSLKVHPDKN+APGS+EAFK +SKAF CLS+D  R++YD +G  D
Sbjct: 121 ESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKKYDVSG-SD 180

Query: 236 QYEYNQQHNVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMF------QTSRAYTYRTGG 295
           +  Y  + + R      G+  +E+ FDP+EI R+FFG G          T++  ++  G 
Sbjct: 181 EPIYQPRRSARSNGFNGGY-YYEDEFDPNEIFRSFFGGGGFGGGGMPPATAQFRSFNFG- 240

Query: 296 AGSQQRTESDGGGP----SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATE 355
             ++QRT ++   P    +  I+L +LP + I LL +MP  +P Y L     Y     T+
Sbjct: 241 -ATRQRTANNNQAPDAGFNARILLQLLPVVFILLLNFMPSSQPVYQLSATYPYQYKFTTQ 300

Query: 356 KHGVEFFVKSSDFDVRYPPGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPH 408
           K GV +FVKSS F+  YP  S  R  +E  V RDY +++ + C  E+QR+QW     TPH
Sbjct: 301 K-GVNYFVKSSKFEQDYPRDSNDRHTLEEQVERDYVSILSQNCRYEMQRKQWGFVRETPH 360

[Close]

BLAST of ClCG06G005080 vs. TAIR 10
Match: AT3G57340.2 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 240.0 bits (611), Expect = 3.4e-63
Identity = 140/370 (37.84%), Postives = 216/370 (58.38%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLTACEEIGSGSPSS 115
           MDGNKD+AL+C+++ + A+ +G++ RAL+F+  A RL+ +L +D+L++      S  P S
Sbjct: 1   MDGNKDDALKCLKICKSAMEAGDRPRALKFLAKARRLDPNLPIDDLVSELNNNKSDEPGS 60

Query: 116 SSDEKRAGKVESVSGSVK--------HVDGLNGERNYTIEHVQLIRQIKTTKDYYRILGV 175
           +     A   +S + S +             +   +YT E + ++R+IK+ KDYY ILG+
Sbjct: 61  AKSPGSAAAKDSSNSSDRPSLRQRGSSTTSSSSSMSYTEEQISIVRKIKSKKDYYEILGL 120

Query: 176 EKTSSAEEIKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLVD 235
           E   S ++++KAY+KLSLKVHPDKN+APGS+EAFK +SKAF CLS+D  R++YD +G  D
Sbjct: 121 ESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKKYDVSG-SD 180

Query: 236 QYEYNQQHNVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMF------QTSRAYTYRTGG 295
           +  Y  + + R      G+  +E+ FDP+EI R+FFG G          T++  ++  G 
Sbjct: 181 EPIYQPRRSARSNGFNGGY-YYEDEFDPNEIFRSFFGGGGFGGGGMPPATAQFRSFNFG- 240

Query: 296 AGSQQRTESDGGGP----SLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYSVPMATE 355
             ++QRT ++   P    +  I+L +LP + I LL +MP  +P Y L     Y     T+
Sbjct: 241 -ATRQRTANNNQAPDAGFNARILLQLLPVVFILLLNFMPSSQPVYQLSATYPYQYKFTTQ 300

Query: 356 KHGVEFFVKSSDFDVRYPPGSPGRAEIENSVLRDYRNMVWRYCHVELQRRQWNRNLPTPH 408
           K GV +FVKSS F+  YP  S  R  +E  V RDY +++ + C  E+QR+QW     TPH
Sbjct: 301 K-GVNYFVKSSKFEQDYPRDSNDRHTLEEQVERDYVSILSQNCRYEMQRKQWGFVRETPH 360

[Close]

BLAST of ClCG06G005080 vs. TAIR 10
Match: AT5G05750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 190.7 bits (483), Expect = 2.4e-48
Identity = 109/285 (38.25%), Postives = 173/285 (60.70%), Query Frame = 0


Query: 56  MDGNKDEALRCIRMAEEAIASGNKERALRFIKIACRLNQSLQVDELLT---------ACE 115
           MDGNKD+AL+C+++ ++AI +G++ RAL+F++ A RL+ +L +D L++         A E
Sbjct: 1   MDGNKDDALKCLKIGKDAIEAGDRSRALKFLEKARRLDPNLPIDGLVSDLKKQSDEPAAE 60

Query: 116 EIGSGSPSSSSDEKRAGKVESVSGSVKHVDGLNGERNYTIEHVQLIRQIKTTKDYYRILG 175
           E   GS ++ S +          GS     G +   + T E   ++R+IK+ KDYY ILG
Sbjct: 61  EDSPGSAANESSKPSDRPSLRQRGSSSSAAGSSSSSSSTEEQRTIVREIKSKKDYYEILG 120

Query: 176 VEKTSSAEEIKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDDTLRRQYDHTGLV 235
           ++   S E+++K+Y+KLSLKVHPDKNKAPGS+EAFK +SKAF CLS++  RR+YD +G  
Sbjct: 121 LKSNCSVEDLRKSYRKLSLKVHPDKNKAPGSEEAFKSVSKAFQCLSNEDTRRKYDGSG-S 180

Query: 236 DQYEYNQQHNVRHRRRRTGHDLFEENFDPDEILRAFFGQGNMFQTSRAYTYRTGGAGSQQ 295
           D+  Y  + + R      G   +++ FD DEI R+FFG G M   +  +     G G++ 
Sbjct: 181 DEPAYQPRRDARRNNGFNG--FYDDEFDADEIFRSFFGGGEMNPATTQFRSFNFGGGTRT 240

Query: 296 RTESDGGGPSLLIILLMLPFLLICLLAYMPFPEPDYALHKNLSYS 332
             ++   G +  ++L +LP + I LL ++P P+P Y+L   ++ S
Sbjct: 241 ANQASDTGFNPRVLLQILPVVFILLLNFLPSPQPIYSLSHRITTS 282

[Close]

BLAST of ClCG06G005080 vs. TAIR 10
Match: AT2G22360.1 (DNAJ heat shock family protein )

HSP 1 Score: 72.4 bits (176), Expect = 9.4e-13
Identity = 33/70 (47.14%), Postives = 46/70 (65.71%), Query Frame = 0


Query: 155 IKTTKDYYRILGVEKTSSAEEIKKAYKKLSLKVHPDKNKAPGSDEAFKKLSKAFMCLSDD 214
           ++   DYY +LGV K ++  EIK AY+KL+   HPD NK PG++E FK++S A+  LSDD
Sbjct: 81  VRADADYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEEKFKEISNAYEVLSDD 140

Query: 215 TLRRQYDHTG 225
             +  YD  G
Sbjct: 141 EKKSLYDRYG 150

The following BLAST results are available for this feature:

BLAST of ClCG06G005080 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038874732.1	1.9e-188	94.60	chaperone protein dnaJ 49 [Benincasa hispida] >XP_038874733.1 chaperone protein ...	[more]
XP_016902270.1	1.6e-179	90.34	PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >XP_016902271.1 PREDICT...	[more]
XP_004148209.1	2.5e-177	89.49	chaperone protein dnaJ 49 [Cucumis sativus] >XP_011657260.1 chaperone protein dn...	[more]
KAG6575070.1	5.9e-174	87.78	Chaperone protein dnaJ 49, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022958924.1	7.6e-174	87.22	chaperone protein dnaJ 49 [Cucurbita moschata] >XP_022958925.1 chaperone protein...	[more]

BLAST of ClCG06G005080 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FH28	3.4e-92	51.13	Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2	[more]
Q7ZXQ8	4.1e-37	32.47	DnaJ homolog subfamily B member 14 OS=Xenopus laevis OX=8355 GN=dnajb14 PE=2 SV=...	[more]
Q28I38	5.4e-37	33.43	DnaJ homolog subfamily B member 14 OS=Xenopus tropicalis OX=8364 GN=dnajb14 PE=2...	[more]
Q149L6	3.5e-36	33.33	DnaJ homolog subfamily B member 14 OS=Mus musculus OX=10090 GN=Dnajb14 PE=2 SV=1	[more]
Q8TBM8	4.5e-36	32.39	DnaJ homolog subfamily B member 14 OS=Homo sapiens OX=9606 GN=DNAJB14 PE=1 SV=1	[more]

BLAST of ClCG06G005080 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4E2R1	7.7e-180	90.34	chaperone protein dnaJ 49-like OS=Cucumis melo OX=3656 GN=LOC103497850 PE=4 SV=1	[more]
A0A0A0KG46	1.2e-177	89.49	J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G306340 PE=4 SV=1	[more]
A0A6J1H4U8	3.7e-174	87.22	chaperone protein dnaJ 49 OS=Cucurbita moschata OX=3662 GN=LOC111460072 PE=4 SV=...	[more]
A0A6J1KXF3	4.1e-173	86.93	chaperone protein dnaJ 49 OS=Cucurbita maxima OX=3661 GN=LOC111499080 PE=4 SV=1	[more]
A0A6J1CB72	5.9e-172	86.78	chaperone protein dnaJ 49 OS=Momordica charantia OX=3673 GN=LOC111009903 PE=4 SV...	[more]

BLAST of ClCG06G005080 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G49060.1	2.4e-93	51.13	Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977)	[more]
AT3G57340.1	3.4e-63	37.84	Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977)	[more]
AT3G57340.2	3.4e-63	37.84	Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977)	[more]
AT5G05750.1	2.4e-48	38.25	DNAJ heat shock N-terminal domain-containing protein	[more]
AT2G22360.1	9.4e-13	47.14	DNAJ heat shock family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 180..195 score: 51.05 coord: 162..180 score: 54.24 coord: 196..216 score: 31.96
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 159..216 e-value: 1.8E-24 score: 97.3
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 160..221 e-value: 3.8E-23 score: 81.4
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 160..224 score: 19.779171
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 160..213 e-value: 7.93465E-21 score: 83.3637
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 157..275 e-value: 1.0E-28 score: 102.0
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 156..236
IPR015399	Domain of unknown function DUF1977, DnaJ-like	PFAM	PF09320	DUF1977	coord: 318..388 e-value: 1.9E-7 score: 31.3
None	No IPR available	PANTHER	PTHR43908	AT29763P-RELATED	coord: 56..406
None	No IPR available	PANTHER	PTHR43908:SF5	CHAPERONE PROTEIN DNAJ 49	coord: 56..406

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG06G005080.1	ClCG06G005080.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071218	cellular response to misfolded protein
biological_process	GO:0051085	chaperone cofactor-dependent protein refolding
biological_process	GO:0030433	ubiquitin-dependent ERAD pathway
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0030544	Hsp70 protein binding