ClCG05G025420 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG05G025420
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Descriptionpolygalacturonase At1g48100-like
LocationCG_Chr05: 36811459 .. 36814780 (-)
RNA-Seq ExpressionClCG05G025420
SyntenyClCG05G025420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAATGAAAGGGATAAGCTTCAGAGGGCTCACATTTATGCTTGTGGTGGCATTTCTTGTCTGGTCTTCCAGTTTGGAAGGGTGCCATGGAAGAGCCACAAGCAAGCATTATTGGAGGCAGAGCAGAGTGGCTTCGCCAACTGTCTCCTCCAAGAAAGGGAAGGCTCATGGTCACGGTGGCTCTCACAAAGGCAACCACCATGGCAGTGCATCAAAGACAAAGCCACCACCGTCTCGTAATACGCCACAACCACTGCCACCACCGGTTACTAAGCCGAAACCAGATGTCCCTCGCAGCCCACCACCTCAGAAGGGAGGGCATTCTTCCATGTTTAACGTGCTGGATTTTGGAGCTAAAGGCGATGGAGAAACTGATGACACTAAGGTGAAACATTAAATCTTTATCTTCATAAAAATAGTAATGAACTTACAATTACACATTATGAGTACGTGTGGTGGAAATGGTATTTTACGGGGAAGGGAAATGGAAAAGGTGGGGTGGGCCCATCTAGAAAGGCGGAATCTCGTTATGGCAGTTGGGTCAGGATTTGAGCAAGAATAATTGGGTGGAGTAGGCAGCATCCTGAAAACTAACGCCAAACAGTTAAAAAAGTCTCTTTTACCGTCAGTAAACAGAGCTAACAGTAAAAGAATCGTGTATATCGCAGGCATTTCAGGACACATGGACAGCCAGTTGCAAGGTGGCAGCATCAACCTTCATCGTCCCATCAAAATACGTCTTTTTAGTAGGCCCCATCTCCTTCTCTGGTCCGTACTGCCAACCCGATATCGTATTCCAGGTACCTAACCTTACACTAACCACAAATTACTCCGACTTTAATTTTACTTGTATCAATTATATCTTTATAGTTTTCAATAATTTTAATGAATCATCCAACCAATTGACATGAACTTAGCAATTTCTTATTGAAATTTAGTTTTCCATCAAAATTAATCCATTCACTATGAGATAGTTAAATACCAAAATCATTATATGAGATGGTGAATAAACTGCAACTATTTCTGTGTTTTTTTTTTTTTTTCATTTAAAAAAAAGTTGGACGGGACAATCATTGCACCGACGGACTTCAAAGTTTGGGGTAAAGGTCTACTGCAATGGCTACAATTCACAAAGTTGATGGGACTTACGATTCAAGGAAATGGGATTATTGATGGAAGGGGTTCAGCATGGTGGCAAGTTTCACCTTATGATGATCCAATTGACGATGAATTCAAACTACTTATCCCACTCAATAGCACTGTACAGGAAAAGCCACAAGCCCCGGTAACTCACCCTTCTTCAAACTCCACATTATGGTTTCTAAGAATTCTCTGCAGCACTTTCTCTAAATTCACACTGAAACCTATGGCAGGCAAGAAGTGAGCTTGTGGGAAAGATGCCTAGGATCAAACCAACTGTAAGCGACATACTAGATCCCTAAATTCCTTGAGCTCAGATCCCTTGACACGGCTCTGTTCTTTTGTTGTACAGGCTTTGAGGTTTTATGGAAGTTTTAACGTTACCGTCACTGGCATAACAATTCAGAACAGTCCTCAATGCCATCTCAAGTTCGACAATTGCATTGGAGTCTTGGTTCATGATTTTAGAGTATCGTCTCCAGGTAACAGTCTCAATACTGATGGAATTCATCTGCAGAATTCCAAGGATGTGCTAATCTACAGCTCCACTCTCTCTTGTGGTATGAATTACTTTGAATAGAATCATTATTTTTGTCACTCTGATATTGTCATTTCGACCATAGTGGGACATGGCATCATTACATGAAAGACTGAAATTTGCATGTATAAGAACCAATCAGTTATAAAAACTATTGGAAAACTCAATGACATCAACCAATGGAGTATTTTTGTCAATTTGAAGAGGTCCGGTGAAATCATAAAGAATTTTTTAGCAACCAGAGGGTTTATCCCTCCCTCACCATCGCCATTGTCCAGCAACCAAACGCAAGACGTGAGGCAAATAGCTAGTTCCCAATCTTCTCCTATTTCTTTGCTATCATTCATGTAACTTGGAGTTTTACACCAGGAAAACTTATGCGCTAATCTTACAAAAATTACCTAATCCTACAACATCTGATTATGAGAAAACTCAATACCTATAGGATTTTTAATATCCTATGTCAGTAGTCACGTTCTAGTTTTTCAATTTTTACTAGCCGGCATAACTTCTGGCTTCAAGCTTCACATTCAACAACCCCACACCCCAAATAAAAGAACAAAATCCTGGCAGTGGCATACATTGGATGAAAACTGACTGCTAGGTTTGATCTTACACACAGGTGACGACTGTATTTCAATACAAACTGGTTGCTCAAATGTGTATATACACAATGTCAATTGTGGTCCAGGACATGGAATCAGCATTGGAAGTCTTGGGAAGGACAACACAAAGGCTTGTGTTTCTAACATCACCGTGCGGGATGTAATTATGCATAACACAATGAATGGTGTCAGAATTAAAACGTGGCAGGTATGCATTTCAAGCACAATTCACCTTTAGACTTAACTCACCAATCGTCCTAGTCTTGATCAGTTAAGTAAATGATTTATTTTGCATTAGTCTGTTATCTTGAGGAAGATAGAAACCTATAAGCAACATGCACTGTAAGATCAAACCAGCTCTCTCAAAATTGATTCCAGAGTGTGCATCTGCAGGGTGGATTAGGTTTTGTACAGGGAGTCCTCTTCTCAAATATTCAGGTTTCTGAGGTTAAGATACCAATAGTTATAGACCAATTCTACTGTGACAAAGCCAAATGCTCAAACCAAACTTCAGCTGTGGCTTTATCAGGAATAAACTATGAAAGAATAAGAGGTACTTATACGGTAAAGCCAGTACACTTTGCCTGTAGTGATAATCTACCATGTACAGATATATCACTAACTGCAATTGAGCTCAAGCCACTGCAAGAACTCTATCATTTATACGGAGCCTTCTGCTGGCAAACTTTTGGAGAGTTGAGAACTCCTACTGTCCCACCAATTGACTGTTTGCAAATTGGGAAGCCATCAAACAATCAAATTCAGCATGATTTTGATTCTTGTTGAGAGTGAAGTCGATAATTATTGGTGTACTCACTTGTGTGGTTGGCATGATTTTCCAACCCCTTTCCAAACTATGAGCCTGTGCTTTGTACATTTTAGTAGTCATGGTTCATAAGATTACTCTATAAAAGTTATAGCTCAGCCCATTGAGATACAAGCATGTTGCTTTGGCAAGAATGCAGTACTAGTATGCTGCTTGTCATTGTACGTTAATTGGGTGACTTTATACTAAGATTCTACGACGGCTTTTAATA

mRNA sequence

ATGAAAATGAAAGGGATAAGCTTCAGAGGGCTCACATTTATGCTTGTGGTGGCATTTCTTGTCTGGTCTTCCAGTTTGGAAGGGTGCCATGGAAGAGCCACAAGCAAGCATTATTGGAGGCAGAGCAGAGTGGCTTCGCCAACTGTCTCCTCCAAGAAAGGGAAGGCTCATGGTCACGGTGGCTCTCACAAAGGCAACCACCATGGCAGTGCATCAAAGACAAAGCCACCACCGTCTCGTAATACGCCACAACCACTGCCACCACCGGTTACTAAGCCGAAACCAGATGTCCCTCGCAGCCCACCACCTCAGAAGGGAGGGCATTCTTCCATGTTTAACGTGCTGGATTTTGGAGCTAAAGGCGATGGAGAAACTGATGACACTAAGGCATTTCAGGACACATGGACAGCCAGTTGCAAGGTGGCAGCATCAACCTTCATCGTCCCATCAAAATACGTCTTTTTAGTAGGCCCCATCTCCTTCTCTGGTCCGTACTGCCAACCCGATATCGTATTCCAGTTGGACGGGACAATCATTGCACCGACGGACTTCAAAGTTTGGGGTAAAGGTCTACTGCAATGGCTACAATTCACAAAGTTGATGGGACTTACGATTCAAGGAAATGGGATTATTGATGGAAGGGGTTCAGCATGGTGGCAAGTTTCACCTTATGATGATCCAATTGACGATGAATTCAAACTACTTATCCCACTCAATAGCACTGTACAGGAAAAGCCACAAGCCCCGGCAAGAAGTGAGCTTGTGGGAAAGATGCCTAGGATCAAACCAACTGCTTTGAGGTTTTATGGAAGTTTTAACGTTACCGTCACTGGCATAACAATTCAGAACAGTCCTCAATGCCATCTCAAGTTCGACAATTGCATTGGAGTCTTGGTTCATGATTTTAGAGTATCGTCTCCAGGTAACAGTCTCAATACTGATGGAATTCATCTGCAGAATTCCAAGGATGTGCTAATCTACAGCTCCACTCTCTCTTGTGTTATAAAAACTATTGGAAAACTCAATGACATCAACCAATGGAGTATTTTTGTCAATTTGAAGAGGTCCGGTGAAATCATAAAGAATTTTTTAGCAACCAGAGGGTTTATCCCTCCCTCACCATCGCCATTGTCCAGCAACCAAACGCAAGACGTGAGGCAAATAGCTAGTTCCCAATCTTCTCCTATTTCTTTGCTATCATTCATGATTTTTAATATCCTATGTGACGACTGTATTTCAATACAAACTGGTTGCTCAAATGTGTATATACACAATGTCAATTGTGGTCCAGGACATGGAATCAGCATTGGAAGTCTTGGGAAGGACAACACAAAGGCTTGTGTTTCTAACATCACCGTGCGGGATGTAATTATGCATAACACAATGAATGGTGTCAGAATTAAAACGTGGCAGGGTGGATTAGGTTTTGTACAGGGAGTCCTCTTCTCAAATATTCAGGTTTCTGAGGTTAAGATACCAATAGTTATAGACCAATTCTACTGTGACAAAGCCAAATGCTCAAACCAAACTTCAGCTGTGGCTTTATCAGGAATAAACTATGAAAGAATAAGAGGTACTTATACGGTAAAGCCAGTACACTTTGCCTGTAGTGATAATCTACCATGTACAGATATATCACTAACTGCAATTGAGCTCAAGCCACTGCAAGAACTCTATCATTTATACGGAGCCTTCTGCTGGCAAACTTTTGGAGAGTTGAGAACTCCTACTGTCCCACCAATTGACTGTTTGCAAATTGGGAAGCCATCAAACAATCAAATTCAGCATGATTTTGATTCTTGTTGAGAGTGAAGTCGATAATTATTGGTGTACTCACTTGTGTGGTTGGCATGATTTTCCAACCCCTTTCCAAACTATGAGCCTGTGCTTTGTACATTTTAGTAGTCATGGTTCATAAGATTACTCTATAAAAGTTATAGCTCAGCCCATTGAGATACAAGCATGTTGCTTTGGCAAGAATGCAGTACTAGTATGCTGCTTGTCATTGTACGTTAATTGGGTGACTTTATACTAAGATTCTACGACGGCTTTTAATA

Coding sequence (CDS)

ATGAAAATGAAAGGGATAAGCTTCAGAGGGCTCACATTTATGCTTGTGGTGGCATTTCTTGTCTGGTCTTCCAGTTTGGAAGGGTGCCATGGAAGAGCCACAAGCAAGCATTATTGGAGGCAGAGCAGAGTGGCTTCGCCAACTGTCTCCTCCAAGAAAGGGAAGGCTCATGGTCACGGTGGCTCTCACAAAGGCAACCACCATGGCAGTGCATCAAAGACAAAGCCACCACCGTCTCGTAATACGCCACAACCACTGCCACCACCGGTTACTAAGCCGAAACCAGATGTCCCTCGCAGCCCACCACCTCAGAAGGGAGGGCATTCTTCCATGTTTAACGTGCTGGATTTTGGAGCTAAAGGCGATGGAGAAACTGATGACACTAAGGCATTTCAGGACACATGGACAGCCAGTTGCAAGGTGGCAGCATCAACCTTCATCGTCCCATCAAAATACGTCTTTTTAGTAGGCCCCATCTCCTTCTCTGGTCCGTACTGCCAACCCGATATCGTATTCCAGTTGGACGGGACAATCATTGCACCGACGGACTTCAAAGTTTGGGGTAAAGGTCTACTGCAATGGCTACAATTCACAAAGTTGATGGGACTTACGATTCAAGGAAATGGGATTATTGATGGAAGGGGTTCAGCATGGTGGCAAGTTTCACCTTATGATGATCCAATTGACGATGAATTCAAACTACTTATCCCACTCAATAGCACTGTACAGGAAAAGCCACAAGCCCCGGCAAGAAGTGAGCTTGTGGGAAAGATGCCTAGGATCAAACCAACTGCTTTGAGGTTTTATGGAAGTTTTAACGTTACCGTCACTGGCATAACAATTCAGAACAGTCCTCAATGCCATCTCAAGTTCGACAATTGCATTGGAGTCTTGGTTCATGATTTTAGAGTATCGTCTCCAGGTAACAGTCTCAATACTGATGGAATTCATCTGCAGAATTCCAAGGATGTGCTAATCTACAGCTCCACTCTCTCTTGTGTTATAAAAACTATTGGAAAACTCAATGACATCAACCAATGGAGTATTTTTGTCAATTTGAAGAGGTCCGGTGAAATCATAAAGAATTTTTTAGCAACCAGAGGGTTTATCCCTCCCTCACCATCGCCATTGTCCAGCAACCAAACGCAAGACGTGAGGCAAATAGCTAGTTCCCAATCTTCTCCTATTTCTTTGCTATCATTCATGATTTTTAATATCCTATGTGACGACTGTATTTCAATACAAACTGGTTGCTCAAATGTGTATATACACAATGTCAATTGTGGTCCAGGACATGGAATCAGCATTGGAAGTCTTGGGAAGGACAACACAAAGGCTTGTGTTTCTAACATCACCGTGCGGGATGTAATTATGCATAACACAATGAATGGTGTCAGAATTAAAACGTGGCAGGGTGGATTAGGTTTTGTACAGGGAGTCCTCTTCTCAAATATTCAGGTTTCTGAGGTTAAGATACCAATAGTTATAGACCAATTCTACTGTGACAAAGCCAAATGCTCAAACCAAACTTCAGCTGTGGCTTTATCAGGAATAAACTATGAAAGAATAAGAGGTACTTATACGGTAAAGCCAGTACACTTTGCCTGTAGTGATAATCTACCATGTACAGATATATCACTAACTGCAATTGAGCTCAAGCCACTGCAAGAACTCTATCATTTATACGGAGCCTTCTGCTGGCAAACTTTTGGAGAGTTGAGAACTCCTACTGTCCCACCAATTGACTGTTTGCAAATTGGGAAGCCATCAAACAATCAAATTCAGCATGATTTTGATTCTTGTTGA

Protein sequence

MKMKGISFRGLTFMLVVAFLVWSSSLEGCHGRATSKHYWRQSRVASPTVSSKKGKAHGHGGSHKGNHHGSASKTKPPPSRNTPQPLPPPVTKPKPDVPRSPPPQKGGHSSMFNVLDFGAKGDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQVSPYDDPIDDEFKLLIPLNSTVQEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWSIFVNLKRSGEIIKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILCDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKPSNNQIQHDFDSC
Homology
BLAST of ClCG05G025420 vs. NCBI nr
Match: XP_038893387.1 (polygalacturonase At1g48100-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1010.4 bits (2611), Expect = 6.6e-291
Identity = 499/601 (83.03%), Postives = 514/601 (85.52%), Query Frame = 0

Query: 1   MKMKGISFRGLTFMLVVAFLVWSSSLEGCHGRATSKHYWRQSRVASPTVSSKKGKAHGHG 60
           MKMKGISFRGLTFM +VAFLVWSSSLEGCHGRATSKHYWR++RVASP +SSKKGKAHGHG
Sbjct: 3   MKMKGISFRGLTFMCLVAFLVWSSSLEGCHGRATSKHYWRKNRVASPALSSKKGKAHGHG 62

Query: 61  GSHKGNHHGSASKTKPPPSRNTPQPLPPPVTKPKPDVPRSPPPQKGGHSSMFNVLDFGAK 120
           GSHKGNHHGSASKTKPPPSR TPQPLPPPV KPKPD+PRSPPPQKGGHSS+FNVLDFGAK
Sbjct: 63  GSHKGNHHGSASKTKPPPSRKTPQPLPPPVAKPKPDIPRSPPPQKGGHSSIFNVLDFGAK 122

Query: 121 GDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA 180
           GDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQP+IVFQLDGTIIA
Sbjct: 123 GDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPNIVFQLDGTIIA 182

Query: 181 PTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQVSPYDDPIDDEFKLLIPLNS 240
           PTDFKVWGKGLLQWLQFTKL GLTIQGNG+IDGRGSAWWQVSPYDDPI+DEFK LIPLNS
Sbjct: 183 PTDFKVWGKGLLQWLQFTKLKGLTIQGNGVIDGRGSAWWQVSPYDDPIEDEFKQLIPLNS 242

Query: 241 TVQEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 300
           T++EKP APARSEL GKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH
Sbjct: 243 TLKEKPPAPARSELEGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 302

Query: 301 DFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWSIFVNLKRSGEII 360
           DF VSSPG+SLNTDGIHLQNSKDVLIYSSTLSC                           
Sbjct: 303 DFFVSSPGDSLNTDGIHLQNSKDVLIYSSTLSC--------------------------- 362

Query: 361 KNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILCDDCISIQTGCSN 420
                                                           DDCISIQTGCSN
Sbjct: 363 -----------------------------------------------GDDCISIQTGCSN 422

Query: 421 VYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGV 480
           VYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRD+ MHNTM GVRIKTWQGGLGFVQGV
Sbjct: 423 VYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDITMHNTMTGVRIKTWQGGLGFVQGV 482

Query: 481 LFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNL 540
           LFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNL
Sbjct: 483 LFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNL 529

Query: 541 PCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKPSNNQIQHDFDS 600
           PC DISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKPS+NQIQ+DFDS
Sbjct: 543 PCADISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKPSSNQIQYDFDS 529

Query: 601 C 602
           C
Sbjct: 603 C 529

BLAST of ClCG05G025420 vs. NCBI nr
Match: XP_038893386.1 (polygalacturonase At1g48100-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1004.2 bits (2595), Expect = 4.7e-289
Identity = 499/606 (82.34%), Postives = 514/606 (84.82%), Query Frame = 0

Query: 1   MKMKGISFRGLTFMLVVAFLVWSSSLEGCHGRATSKHYWRQSRVASPTVSSKKGKAHGHG 60
           MKMKGISFRGLTFM +VAFLVWSSSLEGCHGRATSKHYWR++RVASP +SSKKGKAHGHG
Sbjct: 3   MKMKGISFRGLTFMCLVAFLVWSSSLEGCHGRATSKHYWRKNRVASPALSSKKGKAHGHG 62

Query: 61  GSHKGNHHGSASKTKPPPSRNTPQPLPPPVTKPKPDVPRSPPPQKGGHSSMFNVLDFGAK 120
           GSHKGNHHGSASKTKPPPSR TPQPLPPPV KPKPD+PRSPPPQKGGHSS+FNVLDFGAK
Sbjct: 63  GSHKGNHHGSASKTKPPPSRKTPQPLPPPVAKPKPDIPRSPPPQKGGHSSIFNVLDFGAK 122

Query: 121 GDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA 180
           GDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQP+IVFQLDGTIIA
Sbjct: 123 GDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPNIVFQLDGTIIA 182

Query: 181 PTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQVSPYDDPIDDEFKLLIPLNS 240
           PTDFKVWGKGLLQWLQFTKL GLTIQGNG+IDGRGSAWWQVSPYDDPI+DEFK LIPLNS
Sbjct: 183 PTDFKVWGKGLLQWLQFTKLKGLTIQGNGVIDGRGSAWWQVSPYDDPIEDEFKQLIPLNS 242

Query: 241 TVQEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 300
           T++EKP APARSEL GKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH
Sbjct: 243 TLKEKPPAPARSELEGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVH 302

Query: 301 DFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWSIFVNLKRSGEII 360
           DF VSSPG+SLNTDGIHLQNSKDVLIYSSTLSC                           
Sbjct: 303 DFFVSSPGDSLNTDGIHLQNSKDVLIYSSTLSC--------------------------- 362

Query: 361 KNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILCDDCISIQTGCSN 420
                                                           DDCISIQTGCSN
Sbjct: 363 -----------------------------------------------GDDCISIQTGCSN 422

Query: 421 VYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTW-----QGGLG 480
           VYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRD+ MHNTM GVRIKTW     QGGLG
Sbjct: 423 VYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDITMHNTMTGVRIKTWQTVHLQGGLG 482

Query: 481 FVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFA 540
           FVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFA
Sbjct: 483 FVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFA 534

Query: 541 CSDNLPCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKPSNNQIQ 600
           CSDNLPC DISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKPS+NQIQ
Sbjct: 543 CSDNLPCADISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKPSSNQIQ 534

Query: 601 HDFDSC 602
           +DFDSC
Sbjct: 603 YDFDSC 534

BLAST of ClCG05G025420 vs. NCBI nr
Match: XP_008446197.1 (PREDICTED: polygalacturonase At1g48100-like [Cucumis melo] >XP_016900181.1 PREDICTED: polygalacturonase At1g48100-like [Cucumis melo] >ADN33828.1 polygalacturonase [Cucumis melo subsp. melo] >TYK15613.1 polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 1003.8 bits (2594), Expect = 6.2e-289
Identity = 499/602 (82.89%), Postives = 511/602 (84.88%), Query Frame = 0

Query: 1   MKMKGISFRGLTFMLVVAFLVWSSSLEGCHGRATSKHYWRQSRVASPTVSSKKGKAHGHG 60
           MKM+GISFRGLTFM  VAFLVWSSSLEGCHGR  SKHYWRQ+RVASP + SKKGKAH HG
Sbjct: 3   MKMEGISFRGLTFMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHSHG 62

Query: 61  GSHKGNHHGSASKTKPPPSRNTPQPLPPPVTKPKPDVPRSPPPQKGGHSSMFNVLDFGAK 120
           GSHKGNHHGSASKTKP PS  TPQPLP PVTKPKP++PRSPPPQKGGHS MFNVLDFGAK
Sbjct: 63  GSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFGAK 122

Query: 121 GDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA 180
           GDG+TDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA
Sbjct: 123 GDGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA 182

Query: 181 PTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQ-VSPYDDPIDDEFKLLIPLN 240
           PTDFKVWGKGLLQWLQFTKLMGLTIQGNG+IDGRGS WWQ +SPYDDPIDDEFKLLIPLN
Sbjct: 183 PTDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIPLN 242

Query: 241 STVQEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV 300
           STVQEKP APARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV
Sbjct: 243 STVQEKPPAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV 302

Query: 301 HDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWSIFVNLKRSGEI 360
           HDF VSSPG+SLNTDGIHLQNSKDVLIYSSTLSC                          
Sbjct: 303 HDFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSC-------------------------- 362

Query: 361 IKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILCDDCISIQTGCS 420
                                                            DDCISIQTGCS
Sbjct: 363 ------------------------------------------------GDDCISIQTGCS 422

Query: 421 NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQG 480
           NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQG
Sbjct: 423 NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQG 482

Query: 481 VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN 540
           VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN
Sbjct: 483 VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN 530

Query: 541 LPCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKPSNNQIQHDFD 600
           LPCTDISLTAIELKPLQELYHLYGA+CWQTFGELRTPTVPPIDCLQIGKPS+NQIQ+DFD
Sbjct: 543 LPCTDISLTAIELKPLQELYHLYGAYCWQTFGELRTPTVPPIDCLQIGKPSSNQIQYDFD 530

Query: 601 SC 602
           SC
Sbjct: 603 SC 530

BLAST of ClCG05G025420 vs. NCBI nr
Match: XP_004135250.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN51797.1 hypothetical protein Csa_008759 [Cucumis sativus])

HSP 1 Score: 992.3 bits (2564), Expect = 1.9e-285
Identity = 493/602 (81.89%), Postives = 508/602 (84.39%), Query Frame = 0

Query: 1   MKMKGISFRGLTFMLVVAFLVWSSSLEGCHGRATSKHYWRQSRVASPTVSSKKGKAHGHG 60
           MKM+GISFRGLTFML VAFLVWSSSLEGCHG+  SKHYWR++RVASP + SKKGKAH HG
Sbjct: 3   MKMEGISFRGLTFMLAVAFLVWSSSLEGCHGKVRSKHYWRKNRVASPALYSKKGKAHSHG 62

Query: 61  GSHKGNHHGSASKTKPPPSRNTPQPLPPPVTKPKPDVPRSPPPQKGGHSSMFNVLDFGAK 120
           GSHKGNHHGSASKTKP PS  TPQPLP PVTKPKP++PRSPPPQK GHS MFNVLDFGAK
Sbjct: 63  GSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKEGHSIMFNVLDFGAK 122

Query: 121 GDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA 180
           GDG+TDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA
Sbjct: 123 GDGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA 182

Query: 181 PTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQ-VSPYDDPIDDEFKLLIPLN 240
           PTDFKVWGKGLLQWLQFTKLMGLTIQGNG+IDGRGS WWQ +SPYDDPIDDEFKLLIPLN
Sbjct: 183 PTDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIPLN 242

Query: 241 STVQEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV 300
           STVQEKP  PARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV
Sbjct: 243 STVQEKPPPPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV 302

Query: 301 HDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWSIFVNLKRSGEI 360
           HDF VSSPG+SLNTDGIHLQNSKDVLIYSSTLSC                          
Sbjct: 303 HDFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSC-------------------------- 362

Query: 361 IKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILCDDCISIQTGCS 420
                                                            DDCISIQTGCS
Sbjct: 363 ------------------------------------------------GDDCISIQTGCS 422

Query: 421 NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQG 480
           NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDV MHNTMNGVRIKTWQGGLGFVQG
Sbjct: 423 NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVTMHNTMNGVRIKTWQGGLGFVQG 482

Query: 481 VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN 540
           VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQT+AVALSGINYERIRGTYTVKPVHFACSDN
Sbjct: 483 VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTAAVALSGINYERIRGTYTVKPVHFACSDN 530

Query: 541 LPCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKPSNNQIQHDFD 600
           LPCTDISLTAIELKPLQELYHLY A+CWQTFGELRTPTVPPIDCLQIGKPS+NQIQ+DFD
Sbjct: 543 LPCTDISLTAIELKPLQELYHLYSAYCWQTFGELRTPTVPPIDCLQIGKPSSNQIQYDFD 530

Query: 601 SC 602
           SC
Sbjct: 603 SC 530

BLAST of ClCG05G025420 vs. NCBI nr
Match: KAA0034307.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 987.6 bits (2552), Expect = 4.6e-284
Identity = 499/633 (78.83%), Postives = 511/633 (80.73%), Query Frame = 0

Query: 1   MKMKGISFRGLTFMLVVAFLVWSSSLEGCHGRATSKHYWRQSRVASPTVSSKKGKAHGHG 60
           MKM+GISFRGLTFM  VAFLVWSSSLEGCHGR  SKHYWRQ+RVASP + SKKGKAH HG
Sbjct: 3   MKMEGISFRGLTFMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHSHG 62

Query: 61  GSHKGNHHGSASKTKPPPSRNTPQPLPPPVTKPKPDVPRSPPPQKGGHSSMFNVLDFGAK 120
           GSHKGNHHGSASKTKP PS  TPQPLP PVTKPKP++PRSPPPQKGGHS MFNVLDFGAK
Sbjct: 63  GSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFGAK 122

Query: 121 GDGETDDTK-------------------------------AFQDTWTASCKVAASTFIVP 180
           GDG+TDDTK                               AFQDTWTASCKVAASTFIVP
Sbjct: 123 GDGKTDDTKYVWWKWYFTGKGNGKGGVGPSRKAESRYGSWAFQDTWTASCKVAASTFIVP 182

Query: 181 SKYVFLVGPISFSGPYCQPDIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNG 240
           SKYVFLVGPISFSGPYCQPDIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNG
Sbjct: 183 SKYVFLVGPISFSGPYCQPDIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNG 242

Query: 241 IIDGRGSAWWQ-VSPYDDPIDDEFKLLIPLNSTVQEKPQAPARSELVGKMPRIKPTALRF 300
           +IDGRGS WWQ +SPYDDPIDDEFKLLIPLNSTVQEKP APARSELVGKMPRIKPTALRF
Sbjct: 243 VIDGRGSVWWQEISPYDDPIDDEFKLLIPLNSTVQEKPPAPARSELVGKMPRIKPTALRF 302

Query: 301 YGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFRVSSPGNSLNTDGIHLQNSKDVLIYS 360
           YGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDF VSSPG+SLNTDGIHLQNSKDVLIYS
Sbjct: 303 YGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHLQNSKDVLIYS 362

Query: 361 STLSCVIKTIGKLNDINQWSIFVNLKRSGEIIKNFLATRGFIPPSPSPLSSNQTQDVRQI 420
           STLSC                                                       
Sbjct: 363 STLSC------------------------------------------------------- 422

Query: 421 ASSQSSPISLLSFMIFNILCDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACV 480
                               DDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACV
Sbjct: 423 -------------------GDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACV 482

Query: 481 SNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSN 540
           SNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSN
Sbjct: 483 SNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSN 542

Query: 541 QTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKPLQELYHLYGAFCWQ 600
           QTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKPLQELYHLYGA+CWQ
Sbjct: 543 QTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKPLQELYHLYGAYCWQ 561

Query: 601 TFGELRTPTVPPIDCLQIGKPSNNQIQHDFDSC 602
           TFGELRTPTVPPIDCLQIGKPS+NQIQ+DFDSC
Sbjct: 603 TFGELRTPTVPPIDCLQIGKPSSNQIQYDFDSC 561

BLAST of ClCG05G025420 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 7.0e-94
Identity = 213/537 (39.66%), Postives = 277/537 (51.58%), Query Frame = 0

Query: 63  HKGNHHGSASKTKPPPSRNTPQPLP---PPVTKPKPDVPRSPPPQKGGHSS--MFNVLDF 122
           HKG  HG      PPP    P PL    PP   P    P SP P  G   S  +F+V  F
Sbjct: 32  HKG-RHGVTHPLPPPP----PPPLETANPPDQVPSDPYP-SPDPAPGDSDSGCVFDVTSF 91

Query: 123 GAKGDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGT 182
           GA GDG  DDT AFQD W A+C V +   + P   VF +    FSGP C+P +VFQLDG 
Sbjct: 92  GAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGP-CKPGLVFQLDGV 151

Query: 183 IIAPTDFKVWGK--GLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQVSPYDDPIDDEFKLL 242
           ++ P   + W +     QWL F +L G T  G G ++G G  WW                
Sbjct: 152 LMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWD--------------- 211

Query: 243 IPLNSTVQEKP-QAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNC 302
           +P       KP + P  S   G  P   PT +RF+ S N+ V G+ IQNSPQ H+KFD C
Sbjct: 212 LPC------KPHRGPDGSSSSG--PCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGC 271

Query: 303 IGVLVHDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWSIFVNLK 362
            GVL+++ ++SSP  S NTDGIHL N++ V IY+S +S                      
Sbjct: 272 QGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVS---------------------- 331

Query: 363 RSGEIIKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILCDDCISI 422
            +G                                                   DDCISI
Sbjct: 332 -NG---------------------------------------------------DDCISI 391

Query: 423 QTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGL 482
            TGCS+V I  V CGP HGISIGSLG  N++ACVSNITVR+ ++ ++ NG+R+KTWQGG 
Sbjct: 392 GTGCSDVDIQGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGT 451

Query: 483 GFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVK--PV 542
           G V  +LF NIQ+  V   I++DQ+YC    C N+TSAV +  + Y  I+GTY V+  P+
Sbjct: 452 GSVSNLLFENIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPI 463

Query: 543 HFACSDNLPCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKP 590
           HFACSD + CT+I+++ +EL P +E   +   FCW  +G+  T T+PPIDCL  G P
Sbjct: 512 HFACSDTVACTNITMSEVELLP-EEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSP 463

BLAST of ClCG05G025420 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.5e-69
Identity = 160/474 (33.76%), Postives = 224/474 (47.26%), Query Frame = 0

Query: 111 MFNVLDFGAKGDGETDDTKAFQDTWTASCKVAAST-FIVPSKYVFLVGPISFSGPYCQPD 170
           + +V +FGAKG+G TDDTKAF D W  +C     T  +VP  Y  L+ PI  SGP C+  
Sbjct: 52  LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGP-CKAR 111

Query: 171 IVFQLDGTIIAPTDFKVW-GKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQVSPYDDPI 230
           +  Q+ GTIIAP D  VW G    +WL F  L  LT++G G ++G G  WW+ S      
Sbjct: 112 LTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERS------ 171

Query: 231 DDEFKLLIPLNSTVQEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCH 290
                         +     P R           PTAL F+   N+ V  + + +S Q H
Sbjct: 172 -------------CKHNHSNPCRG---------APTALTFHKCKNMRVENLNVIDSQQMH 231

Query: 291 LKFDNCIGVLVHDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWS 350
           +   +C  V +   +V +P  S NTDGIH+  S+ ++I ++T+S                
Sbjct: 232 IALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVS---------------- 291

Query: 351 IFVNLKRSGEIIKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILC 410
                                                                       
Sbjct: 292 ----------------------------------------------------------TG 351

Query: 411 DDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIK 470
           DDCISI    + + I N+ CGPGHGISIGSLGK  +   V +ITV   I+ +T NGVRIK
Sbjct: 352 DDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVRIK 411

Query: 471 TWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAK-CSNQTSAVALSGINYERIRGTY 530
           TWQGG G V  ++F NI+++ V  PI+IDQ+YCD  K C+NQTSA+++  I++  +RGT 
Sbjct: 412 TWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRGTS 421

Query: 531 TVK-PVHFACSDNLPCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPP 581
             K  +  +CSD+ PC +I L  I+L+P         +FCW+ +G       PP
Sbjct: 472 ASKEAIKISCSDSSPCRNILLQDIDLEP-SNGDGFTESFCWEAYGSSSGQVYPP 421

BLAST of ClCG05G025420 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 9.6e-67
Identity = 155/447 (34.68%), Postives = 216/447 (48.32%), Query Frame = 0

Query: 109 SSMFNVLDFGAKGDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQP 168
           +++FNV  +GA GDG+ D T+AF  TW A+CK A++  +VP+   F V  + F GP CQP
Sbjct: 56  ATVFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGP-CQP 115

Query: 169 DIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQVSPYDDPI 228
            + F++DGTI+A  D   W K    WLQF +L    + G G+IDG+G  WW         
Sbjct: 116 HLSFKVDGTIVAQPDPARW-KNSKIWLQFAQLTDFNLMGTGVIDGQGQQWW--------- 175

Query: 229 DDEFKLLIPLNSTVQEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCH 288
                             Q    +       R +PTA++   S +VTV  +T+ NSP+ H
Sbjct: 176 ----------------AGQCKVVNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFH 235

Query: 289 LKFDNCIGVLVHDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWS 348
           L F  C GV +   ++ +P +S NTDGI +  SK   I      CVI T           
Sbjct: 236 LVFGECEGVKIQGLKIKAPRDSPNTDGIDIFASKRFHIE----KCVIGT----------- 295

Query: 349 IFVNLKRSGEIIKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILC 408
                                                                       
Sbjct: 296 -----------------------------------------------------------G 355

Query: 409 DDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIK 468
           DDCI+I TG SN+ I ++ CGPGHGISIGSLG+DN++A VS++ V      +T NG+RIK
Sbjct: 356 DDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIK 401

Query: 469 TWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAK-CSNQTSAVALSGINYERIRGT- 528
           TWQGG G    + + N+++   + PI+I+QFYC  A  C NQ SAV + G+ Y+ I GT 
Sbjct: 416 TWQGGSGLASYITYENVEMINSENPILINQFYCTSASACQNQRSAVQIQGVTYKNIHGTS 401

Query: 529 YTVKPVHFACSDNLPCTDISLTAIELK 554
            T   +   CSD++PCT I L+ + LK
Sbjct: 476 ATAAAIQLMCSDSVPCTGIQLSNVSLK 401

BLAST of ClCG05G025420 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 2.0e-64
Identity = 148/449 (32.96%), Postives = 215/449 (47.88%), Query Frame = 0

Query: 110 SMFNVLDFGAKGDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPD 169
           ++FNV  +GA GDG+ D T+AF   W A+CK  ++  +VP    F+V  + F+GP CQP 
Sbjct: 57  NIFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGP-CQPH 116

Query: 170 IVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQVSPYDDPID 229
             F++DG I A  +   W    + WLQF KL G T+ G G+IDG+G  WW          
Sbjct: 117 FTFKVDGIIAAYQNPASWKNNRI-WLQFAKLTGFTLMGKGVIDGQGKQWW---------- 176

Query: 230 DEFKLLIPLNSTVQEKPQAPARSELVGK---MPRIKPTALRFYGSFNVTVTGITIQNSPQ 289
                             A     + G+     R +PTA++F  S  + + G+ + NSP+
Sbjct: 177 ------------------AGQCKWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPE 236

Query: 290 CHLKFDNCIGVLVHDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQ 349
            HL F NC GV +    +++P +S NTDGI +  SK+  +  +T+               
Sbjct: 237 FHLVFGNCEGVKIIGISITAPRDSPNTDGIDIFASKNFHLQKNTIG-------------- 296

Query: 350 WSIFVNLKRSGEIIKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNI 409
                                                                       
Sbjct: 297 ------------------------------------------------------------ 356

Query: 410 LCDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVR 469
             DDC++I TG SN+ I ++ CGPGHGISIGSLG++N++A VS + V      +T NG+R
Sbjct: 357 TGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSRAEVSYVHVNGAKFIDTQNGLR 401

Query: 470 IKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAK-CSNQTSAVALSGINYERIRG 529
           IKTWQGG G    +++ N+++   + PI+I+QFYC  A  C NQ SAV +  + Y+ IRG
Sbjct: 417 IKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSASACQNQRSAVQIQDVTYKNIRG 401

Query: 530 T-YTVKPVHFACSDNLPCTDISLTAIELK 554
           T  T   +   CSD++PC DI L+ I LK
Sbjct: 477 TSATAAAIQLKCSDSMPCKDIKLSDISLK 401

BLAST of ClCG05G025420 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 2.0e-64
Identity = 154/447 (34.45%), Postives = 211/447 (47.20%), Query Frame = 0

Query: 109 SSMFNVLDFGAKGDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQP 168
           S   NV DFGAKGDG  DDTKAF+  W A+C   +S  ++  K  +LV PISFSGP C+ 
Sbjct: 87  SKTVNVDDFGAKGDGR-DDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGP-CKS 146

Query: 169 DIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQVSPYDDPI 228
            +  Q+ GTI A  D   + K    WL F  +  L ++G G I+G G  WWQ        
Sbjct: 147 GLTMQIYGTIEASDDRSDYRKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQ-------- 206

Query: 229 DDEFKLLIPLNSTVQEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCH 288
                      ++ +     P +           PTAL FY S +V V  + I+N+ Q H
Sbjct: 207 -----------NSCKTNKALPCKD---------APTALTFYKSKHVIVKNLKIENAQQIH 266

Query: 289 LKFDNCIGVLVHDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWS 348
           + FDNC+ V   +  V++P NS NTDGIH+  ++++ I     SCVI T           
Sbjct: 267 VSFDNCVNVQASNLMVTAPENSPNTDGIHVTGTQNIHI----SSCVIGT----------- 326

Query: 349 IFVNLKRSGEIIKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILC 408
                                                                       
Sbjct: 327 -----------------------------------------------------------G 386

Query: 409 DDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIK 468
           DDCISI  G   V ++++ CGPGHGISIGSLG  N++A VS++ V    +  T NGVRIK
Sbjct: 387 DDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHVSDVVVNGAKLCGTTNGVRIK 429

Query: 469 TWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAK-CSNQTSAVALSGINYERIRGTY 528
           TWQGG G    + F N+++  V+ PI+IDQ YCD+ K C  Q+SAV +  + Y+ I+GT 
Sbjct: 447 TWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKPCQEQSSAVQVKNVVYQNIKGTC 429

Query: 529 TVK-PVHFACSDNLPCTDISLTAIELK 554
                + F CS   PC  I L  ++L+
Sbjct: 507 ASNVAITFDCSKRFPCQGIVLEDVDLE 429

BLAST of ClCG05G025420 vs. ExPASy TrEMBL
Match: A0A1S3BFB4 (polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103489001 PE=3 SV=1)

HSP 1 Score: 1003.8 bits (2594), Expect = 3.0e-289
Identity = 499/602 (82.89%), Postives = 511/602 (84.88%), Query Frame = 0

Query: 1   MKMKGISFRGLTFMLVVAFLVWSSSLEGCHGRATSKHYWRQSRVASPTVSSKKGKAHGHG 60
           MKM+GISFRGLTFM  VAFLVWSSSLEGCHGR  SKHYWRQ+RVASP + SKKGKAH HG
Sbjct: 3   MKMEGISFRGLTFMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHSHG 62

Query: 61  GSHKGNHHGSASKTKPPPSRNTPQPLPPPVTKPKPDVPRSPPPQKGGHSSMFNVLDFGAK 120
           GSHKGNHHGSASKTKP PS  TPQPLP PVTKPKP++PRSPPPQKGGHS MFNVLDFGAK
Sbjct: 63  GSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFGAK 122

Query: 121 GDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA 180
           GDG+TDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA
Sbjct: 123 GDGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA 182

Query: 181 PTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQ-VSPYDDPIDDEFKLLIPLN 240
           PTDFKVWGKGLLQWLQFTKLMGLTIQGNG+IDGRGS WWQ +SPYDDPIDDEFKLLIPLN
Sbjct: 183 PTDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIPLN 242

Query: 241 STVQEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV 300
           STVQEKP APARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV
Sbjct: 243 STVQEKPPAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV 302

Query: 301 HDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWSIFVNLKRSGEI 360
           HDF VSSPG+SLNTDGIHLQNSKDVLIYSSTLSC                          
Sbjct: 303 HDFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSC-------------------------- 362

Query: 361 IKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILCDDCISIQTGCS 420
                                                            DDCISIQTGCS
Sbjct: 363 ------------------------------------------------GDDCISIQTGCS 422

Query: 421 NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQG 480
           NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQG
Sbjct: 423 NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQG 482

Query: 481 VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN 540
           VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN
Sbjct: 483 VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN 530

Query: 541 LPCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKPSNNQIQHDFD 600
           LPCTDISLTAIELKPLQELYHLYGA+CWQTFGELRTPTVPPIDCLQIGKPS+NQIQ+DFD
Sbjct: 543 LPCTDISLTAIELKPLQELYHLYGAYCWQTFGELRTPTVPPIDCLQIGKPSSNQIQYDFD 530

Query: 601 SC 602
           SC
Sbjct: 603 SC 530

BLAST of ClCG05G025420 vs. ExPASy TrEMBL
Match: A0A5D3CUP5 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001100 PE=3 SV=1)

HSP 1 Score: 1003.8 bits (2594), Expect = 3.0e-289
Identity = 499/602 (82.89%), Postives = 511/602 (84.88%), Query Frame = 0

Query: 1   MKMKGISFRGLTFMLVVAFLVWSSSLEGCHGRATSKHYWRQSRVASPTVSSKKGKAHGHG 60
           MKM+GISFRGLTFM  VAFLVWSSSLEGCHGR  SKHYWRQ+RVASP + SKKGKAH HG
Sbjct: 3   MKMEGISFRGLTFMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHSHG 62

Query: 61  GSHKGNHHGSASKTKPPPSRNTPQPLPPPVTKPKPDVPRSPPPQKGGHSSMFNVLDFGAK 120
           GSHKGNHHGSASKTKP PS  TPQPLP PVTKPKP++PRSPPPQKGGHS MFNVLDFGAK
Sbjct: 63  GSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFGAK 122

Query: 121 GDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA 180
           GDG+TDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA
Sbjct: 123 GDGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA 182

Query: 181 PTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQ-VSPYDDPIDDEFKLLIPLN 240
           PTDFKVWGKGLLQWLQFTKLMGLTIQGNG+IDGRGS WWQ +SPYDDPIDDEFKLLIPLN
Sbjct: 183 PTDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIPLN 242

Query: 241 STVQEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV 300
           STVQEKP APARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV
Sbjct: 243 STVQEKPPAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV 302

Query: 301 HDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWSIFVNLKRSGEI 360
           HDF VSSPG+SLNTDGIHLQNSKDVLIYSSTLSC                          
Sbjct: 303 HDFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSC-------------------------- 362

Query: 361 IKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILCDDCISIQTGCS 420
                                                            DDCISIQTGCS
Sbjct: 363 ------------------------------------------------GDDCISIQTGCS 422

Query: 421 NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQG 480
           NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQG
Sbjct: 423 NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQG 482

Query: 481 VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN 540
           VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN
Sbjct: 483 VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN 530

Query: 541 LPCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKPSNNQIQHDFD 600
           LPCTDISLTAIELKPLQELYHLYGA+CWQTFGELRTPTVPPIDCLQIGKPS+NQIQ+DFD
Sbjct: 543 LPCTDISLTAIELKPLQELYHLYGAYCWQTFGELRTPTVPPIDCLQIGKPSSNQIQYDFD 530

Query: 601 SC 602
           SC
Sbjct: 603 SC 530

BLAST of ClCG05G025420 vs. ExPASy TrEMBL
Match: E5GBI6 (Polygalacturonase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1003.8 bits (2594), Expect = 3.0e-289
Identity = 499/602 (82.89%), Postives = 511/602 (84.88%), Query Frame = 0

Query: 1   MKMKGISFRGLTFMLVVAFLVWSSSLEGCHGRATSKHYWRQSRVASPTVSSKKGKAHGHG 60
           MKM+GISFRGLTFM  VAFLVWSSSLEGCHGR  SKHYWRQ+RVASP + SKKGKAH HG
Sbjct: 3   MKMEGISFRGLTFMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHSHG 62

Query: 61  GSHKGNHHGSASKTKPPPSRNTPQPLPPPVTKPKPDVPRSPPPQKGGHSSMFNVLDFGAK 120
           GSHKGNHHGSASKTKP PS  TPQPLP PVTKPKP++PRSPPPQKGGHS MFNVLDFGAK
Sbjct: 63  GSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFGAK 122

Query: 121 GDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA 180
           GDG+TDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA
Sbjct: 123 GDGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA 182

Query: 181 PTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQ-VSPYDDPIDDEFKLLIPLN 240
           PTDFKVWGKGLLQWLQFTKLMGLTIQGNG+IDGRGS WWQ +SPYDDPIDDEFKLLIPLN
Sbjct: 183 PTDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIPLN 242

Query: 241 STVQEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV 300
           STVQEKP APARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV
Sbjct: 243 STVQEKPPAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV 302

Query: 301 HDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWSIFVNLKRSGEI 360
           HDF VSSPG+SLNTDGIHLQNSKDVLIYSSTLSC                          
Sbjct: 303 HDFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSC-------------------------- 362

Query: 361 IKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILCDDCISIQTGCS 420
                                                            DDCISIQTGCS
Sbjct: 363 ------------------------------------------------GDDCISIQTGCS 422

Query: 421 NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQG 480
           NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQG
Sbjct: 423 NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQG 482

Query: 481 VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN 540
           VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN
Sbjct: 483 VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN 530

Query: 541 LPCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKPSNNQIQHDFD 600
           LPCTDISLTAIELKPLQELYHLYGA+CWQTFGELRTPTVPPIDCLQIGKPS+NQIQ+DFD
Sbjct: 543 LPCTDISLTAIELKPLQELYHLYGAYCWQTFGELRTPTVPPIDCLQIGKPSSNQIQYDFD 530

Query: 601 SC 602
           SC
Sbjct: 603 SC 530

BLAST of ClCG05G025420 vs. ExPASy TrEMBL
Match: A0A0A0KTN9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G601470 PE=3 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 9.0e-286
Identity = 493/602 (81.89%), Postives = 508/602 (84.39%), Query Frame = 0

Query: 1   MKMKGISFRGLTFMLVVAFLVWSSSLEGCHGRATSKHYWRQSRVASPTVSSKKGKAHGHG 60
           MKM+GISFRGLTFML VAFLVWSSSLEGCHG+  SKHYWR++RVASP + SKKGKAH HG
Sbjct: 3   MKMEGISFRGLTFMLAVAFLVWSSSLEGCHGKVRSKHYWRKNRVASPALYSKKGKAHSHG 62

Query: 61  GSHKGNHHGSASKTKPPPSRNTPQPLPPPVTKPKPDVPRSPPPQKGGHSSMFNVLDFGAK 120
           GSHKGNHHGSASKTKP PS  TPQPLP PVTKPKP++PRSPPPQK GHS MFNVLDFGAK
Sbjct: 63  GSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKEGHSIMFNVLDFGAK 122

Query: 121 GDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA 180
           GDG+TDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA
Sbjct: 123 GDGKTDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIA 182

Query: 181 PTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQ-VSPYDDPIDDEFKLLIPLN 240
           PTDFKVWGKGLLQWLQFTKLMGLTIQGNG+IDGRGS WWQ +SPYDDPIDDEFKLLIPLN
Sbjct: 183 PTDFKVWGKGLLQWLQFTKLMGLTIQGNGVIDGRGSVWWQEISPYDDPIDDEFKLLIPLN 242

Query: 241 STVQEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV 300
           STVQEKP  PARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV
Sbjct: 243 STVQEKPPPPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLV 302

Query: 301 HDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWSIFVNLKRSGEI 360
           HDF VSSPG+SLNTDGIHLQNSKDVLIYSSTLSC                          
Sbjct: 303 HDFNVSSPGDSLNTDGIHLQNSKDVLIYSSTLSC-------------------------- 362

Query: 361 IKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILCDDCISIQTGCS 420
                                                            DDCISIQTGCS
Sbjct: 363 ------------------------------------------------GDDCISIQTGCS 422

Query: 421 NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQG 480
           NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDV MHNTMNGVRIKTWQGGLGFVQG
Sbjct: 423 NVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVTMHNTMNGVRIKTWQGGLGFVQG 482

Query: 481 VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDN 540
           VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQT+AVALSGINYERIRGTYTVKPVHFACSDN
Sbjct: 483 VLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTAAVALSGINYERIRGTYTVKPVHFACSDN 530

Query: 541 LPCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKPSNNQIQHDFD 600
           LPCTDISLTAIELKPLQELYHLY A+CWQTFGELRTPTVPPIDCLQIGKPS+NQIQ+DFD
Sbjct: 543 LPCTDISLTAIELKPLQELYHLYSAYCWQTFGELRTPTVPPIDCLQIGKPSSNQIQYDFD 530

Query: 601 SC 602
           SC
Sbjct: 603 SC 530

BLAST of ClCG05G025420 vs. ExPASy TrEMBL
Match: A0A5A7SYF1 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003070 PE=3 SV=1)

HSP 1 Score: 987.6 bits (2552), Expect = 2.2e-284
Identity = 499/633 (78.83%), Postives = 511/633 (80.73%), Query Frame = 0

Query: 1   MKMKGISFRGLTFMLVVAFLVWSSSLEGCHGRATSKHYWRQSRVASPTVSSKKGKAHGHG 60
           MKM+GISFRGLTFM  VAFLVWSSSLEGCHGR  SKHYWRQ+RVASP + SKKGKAH HG
Sbjct: 3   MKMEGISFRGLTFMFAVAFLVWSSSLEGCHGRVRSKHYWRQNRVASPALYSKKGKAHSHG 62

Query: 61  GSHKGNHHGSASKTKPPPSRNTPQPLPPPVTKPKPDVPRSPPPQKGGHSSMFNVLDFGAK 120
           GSHKGNHHGSASKTKP PS  TPQPLP PVTKPKP++PRSPPPQKGGHS MFNVLDFGAK
Sbjct: 63  GSHKGNHHGSASKTKPRPSHKTPQPLPRPVTKPKPNIPRSPPPQKGGHSFMFNVLDFGAK 122

Query: 121 GDGETDDTK-------------------------------AFQDTWTASCKVAASTFIVP 180
           GDG+TDDTK                               AFQDTWTASCKVAASTFIVP
Sbjct: 123 GDGKTDDTKYVWWKWYFTGKGNGKGGVGPSRKAESRYGSWAFQDTWTASCKVAASTFIVP 182

Query: 181 SKYVFLVGPISFSGPYCQPDIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNG 240
           SKYVFLVGPISFSGPYCQPDIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNG
Sbjct: 183 SKYVFLVGPISFSGPYCQPDIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNG 242

Query: 241 IIDGRGSAWWQ-VSPYDDPIDDEFKLLIPLNSTVQEKPQAPARSELVGKMPRIKPTALRF 300
           +IDGRGS WWQ +SPYDDPIDDEFKLLIPLNSTVQEKP APARSELVGKMPRIKPTALRF
Sbjct: 243 VIDGRGSVWWQEISPYDDPIDDEFKLLIPLNSTVQEKPPAPARSELVGKMPRIKPTALRF 302

Query: 301 YGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFRVSSPGNSLNTDGIHLQNSKDVLIYS 360
           YGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDF VSSPG+SLNTDGIHLQNSKDVLIYS
Sbjct: 303 YGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDFNVSSPGDSLNTDGIHLQNSKDVLIYS 362

Query: 361 STLSCVIKTIGKLNDINQWSIFVNLKRSGEIIKNFLATRGFIPPSPSPLSSNQTQDVRQI 420
           STLSC                                                       
Sbjct: 363 STLSC------------------------------------------------------- 422

Query: 421 ASSQSSPISLLSFMIFNILCDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACV 480
                               DDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACV
Sbjct: 423 -------------------GDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACV 482

Query: 481 SNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSN 540
           SNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSN
Sbjct: 483 SNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSN 542

Query: 541 QTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKPLQELYHLYGAFCWQ 600
           QTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKPLQELYHLYGA+CWQ
Sbjct: 543 QTSAVALSGINYERIRGTYTVKPVHFACSDNLPCTDISLTAIELKPLQELYHLYGAYCWQ 561

Query: 601 TFGELRTPTVPPIDCLQIGKPSNNQIQHDFDSC 602
           TFGELRTPTVPPIDCLQIGKPS+NQIQ+DFDSC
Sbjct: 603 TFGELRTPTVPPIDCLQIGKPSSNQIQYDFDSC 561

BLAST of ClCG05G025420 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 679.9 bits (1753), Expect = 1.9e-195
Identity = 343/615 (55.77%), Postives = 416/615 (67.64%), Query Frame = 0

Query: 4   KGISFRGLTFMLVVAFLVWSSSLEGCHGRATS--KHYWRQSRVASPTVSSKKGKAHGHGG 63
           + ++ R +T M +V  LVWS++LE C  R     +H+ R S   S ++SSKK K      
Sbjct: 5   ESLTLRSITMMTLVTILVWSATLETCIARRGRHWRHHHRSSSSLSDSLSSKKPK------ 64

Query: 64  SHKGNHHGSASKTKPPPSRNTPQPLP---PPVTKPKPDVPRSPPP-----------QKGG 123
           SH+ NHH  + K+KP P   T  P      PV    P V + PPP           Q   
Sbjct: 65  SHENNHHHMSHKSKPKPKMKTQPPKSNDGSPVVSQPPQVQQPPPPHVQPPPTPLPLQPVE 124

Query: 124 HSSMFNVLDFGAKGDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQ 183
            S  FNVLDFGAKGDG +DDT+AF+  W ++CKV AST I+P  Y+FLVGPISFSGPYCQ
Sbjct: 125 DSQQFNVLDFGAKGDGMSDDTQAFEAAWASACKVEASTMIIPPDYIFLVGPISFSGPYCQ 184

Query: 184 PDIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQVSPYDD- 243
            +IVFQL+G I+APTD + WG GL+ W++FTKL G+TIQGNG+IDGRG+ WWQ     D 
Sbjct: 185 ANIVFQLEGMIVAPTDTESWGGGLMWWIEFTKLSGITIQGNGVIDGRGTVWWQQDYLSDY 244

Query: 244 PIDDEFKLLIPLNSTVQEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQ 303
           PIDD+FKL++PLN++VQE+P  P RSEL  +MP IKPTALRFYGS +VTVTGITIQNSPQ
Sbjct: 245 PIDDDFKLIVPLNNSVQERPPMPIRSELNWRMPSIKPTALRFYGSIDVTVTGITIQNSPQ 304

Query: 304 CHLKFDNCIGVLVHDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQ 363
           CHLKFDNC+ VLVHD  VSSPG+S NTDGIHLQN++DV+I+++TL+C             
Sbjct: 305 CHLKFDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLAC------------- 364

Query: 364 WSIFVNLKRSGEIIKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNI 423
                                                                       
Sbjct: 365 ------------------------------------------------------------ 424

Query: 424 LCDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVR 483
             DDCISIQTGCSNVY+HNVNCGPGHGISIGSLGKD+TKACVSNITVRDV+MHNTM GVR
Sbjct: 425 -GDDCISIQTGCSNVYVHNVNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVR 484

Query: 484 IKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGT 543
           IKTWQGG+G V+G+LFSNIQ++EV++PIVIDQFYCD +KC N TSAV++ G+ YE+IRGT
Sbjct: 485 IKTWQGGIGSVKGILFSNIQLTEVQLPIVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGT 539

Query: 544 YTVKPVHFACSDNLPCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQI 602
           YTVKPVHFACSD+ PC D+ L+ IELKP+Q  YH+Y  FCW+TFGEL + TVPPIDCLQI
Sbjct: 545 YTVKPVHFACSDSFPCIDVQLSGIELKPVQLQYHMYDPFCWKTFGELNSATVPPIDCLQI 539

BLAST of ClCG05G025420 vs. TAIR 10
Match: AT1G10640.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 676.8 bits (1745), Expect = 1.6e-194
Identity = 345/608 (56.74%), Postives = 414/608 (68.09%), Query Frame = 0

Query: 4   KGISFRGLTFMLVVAFLVWSSSLEGCHGRATS--KHYWRQSRVASPTVSSKKGKAHG--- 63
           + +S R +T M+++A LVWS +LE C  R     +H  R S   S ++S+KK K+HG   
Sbjct: 5   RSLSLRSITMMILMAVLVWSVTLETCIARRGRHWRHSHRSSSDLSDSLSNKKPKSHGNSH 64

Query: 64  HGGSHKGNHHGSASKTKPPPSRNTPQPL----PPPVTKPKPDVPRSPPPQKGGHSSMFNV 123
           H  SH  NHH   SK KP P   TP        P V+ P    P   PPQKG  S +FNV
Sbjct: 65  HHSSHNNNHH-HKSKPKPKPKLKTPPKAHDNNSPVVSGPPKVQPPPLPPQKG--SQVFNV 124

Query: 124 LDFGAKGDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQL 183
           +DFGAKGDG+ DDTKAF+  W A+CK+ AS  +VP +Y +LVGPISFSGPYCQ +IVFQL
Sbjct: 125 MDFGAKGDGKCDDTKAFEAAWVAACKIEASVMLVPPEYTYLVGPISFSGPYCQANIVFQL 184

Query: 184 DGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQVSPYDDP-IDDEFK 243
           DGTIIAPTD K WGKGL+ W+ FTKL G+ +QG G+IDGRGS WWQ    D P ID + K
Sbjct: 185 DGTIIAPTDSKTWGKGLMWWIDFTKLKGIKVQGKGVIDGRGSGWWQ---QDSPFIDSDTK 244

Query: 244 LLIPLNSTVQEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDN 303
           L++PLN++  + P  P RSEL  +MP IKPTALRF GSF V VTGITIQNSPQCHLKFD+
Sbjct: 245 LIVPLNNSANQNPPMPIRSELDERMPSIKPTALRFSGSFGVEVTGITIQNSPQCHLKFDD 304

Query: 304 CIGVLVHDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWSIFVNL 363
           C+GV+VHD  VSSPG+S NTDGIHLQN+KDVLI+S+TL+C                    
Sbjct: 305 CVGVVVHDIAVSSPGDSPNTDGIHLQNTKDVLIHSTTLAC-------------------- 364

Query: 364 KRSGEIIKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILCDDCIS 423
                                                                  DDCIS
Sbjct: 365 ------------------------------------------------------GDDCIS 424

Query: 424 IQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGG 483
           IQTGCSNV++HNVNCGPGHGISIGSLGK+ TKACVSNITVRDV MHNTM GVRIKTWQGG
Sbjct: 425 IQTGCSNVFVHNVNCGPGHGISIGSLGKEGTKACVSNITVRDVAMHNTMTGVRIKTWQGG 484

Query: 484 LGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVH 543
           +G V+G++FSNIQ+++V+IPI I+QFYCD +KC NQTSAVA+ G+ YERI+GTYTVKPVH
Sbjct: 485 VGSVKGIIFSNIQLNQVQIPITINQFYCDHSKCKNQTSAVAVEGVTYERIKGTYTVKPVH 532

Query: 544 FACSDNLPCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKPSNNQ 602
           FACSDN PC D+ L++IELKP+QE Y +Y A+CWQTFGEL TPT+PPIDCL+IGKP  N+
Sbjct: 545 FACSDNFPCVDVQLSSIELKPVQEKYRMYDAYCWQTFGELNTPTLPPIDCLKIGKPPRNK 532

BLAST of ClCG05G025420 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 500.7 bits (1288), Expect = 1.6e-141
Identity = 260/498 (52.21%), Postives = 303/498 (60.84%), Query Frame = 0

Query: 105 KGGHSSMFNVLDFGAKGDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGP 164
           +GG S   NVLD GAKGDG +DDTKAF+D W  +CKVAAST +VPS   FLVGP+SF G 
Sbjct: 44  EGGSSGTINVLDHGAKGDGTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSFLGK 103

Query: 165 YCQPDIVFQLDGTIIAPTDFKVWGKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQVSPY 224
            C+  IVFQL+G IIAPT    WG GLLQW++F  L G+TI+G GIIDGRGS WW     
Sbjct: 104 ECKEKIVFQLEGKIIAPTSASAWGSGLLQWIEFKALQGITIKGKGIIDGRGSVWW----- 163

Query: 225 DDPIDDEFKLLIPLNSTVQEKPQAPARSELVG-KMPRIKPTALRFYGSFNVTVTGITIQN 284
                                      ++++G KMPR KPTALRFYGS  VTV+GITIQN
Sbjct: 164 ---------------------------NDMMGTKMPRTKPTALRFYGSNGVTVSGITIQN 223

Query: 285 SPQCHLKFDNCIGVLVHDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLND 344
           SPQ HLKFDNCI + V DF  SSPG+S NTDGIHLQNS+D +IY STL+C          
Sbjct: 224 SPQTHLKFDNCISIQVSDFTTSSPGDSPNTDGIHLQNSQDAVIYRSTLAC---------- 283

Query: 345 INQWSIFVNLKRSGEIIKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMI 404
                                                                       
Sbjct: 284 ------------------------------------------------------------ 343

Query: 405 FNILCDDCISIQTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMN 464
                DDCISIQTGCSN+ IH+V+CGPGHGISIG LGKDNTKACVSNITVRDV MH T N
Sbjct: 344 ----GDDCISIQTGCSNINIHDVDCGPGHGISIGGLGKDNTKACVSNITVRDVTMHETTN 403

Query: 465 GVRIKTWQGGLGFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERI 524
           GVRIK+WQGG G V+ V+FSNIQVS V  PI+IDQ+YCD   C N+TSAVA+S INY  I
Sbjct: 404 GVRIKSWQGGSGSVKQVMFSNIQVSNVANPIIIDQYYCDGGGCHNETSAVAVSNINYINI 435

Query: 525 RGTYTVKPVHFACSDNLPCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDC 584
           +GTYT +PV FACSD+LPCT ISL+ IELKP          FCW+  GEL+T T+PPI C
Sbjct: 464 KGTYTKEPVRFACSDSLPCTGISLSTIELKPATGKASSLDPFCWKAHGELKTKTLPPIQC 435

Query: 585 LQIGKPSNNQIQHDFDSC 602
           L+  K      + + D+C
Sbjct: 524 LKTEKSPEAASRSNNDAC 435

BLAST of ClCG05G025420 vs. TAIR 10
Match: AT3G26610.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 372.1 bits (954), Expect = 8.5e-103
Identity = 209/522 (40.04%), Postives = 278/522 (53.26%), Query Frame = 0

Query: 67  HHGSASKTKPP---PSRNTPQPLPPPVTKPKPDVPRSPPPQKGGHSSMFNVLDFGAKGDG 126
           HH    K KP     S+NTP   P            +P P    +  +FN+  +GAKGDG
Sbjct: 33  HH--VKKIKPKHRRHSKNTPTGSP------------APAPYPSTNEGVFNIFSYGAKGDG 92

Query: 127 ETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGTIIAPTD 186
            +DD+KA    W A+CKV      +P+   FLV  ++  GP C+ + V Q++G ++AP  
Sbjct: 93  VSDDSKALVGAWKAACKVVGGKVEIPAGTQFLVKAVTLQGP-CKEETVVQIEGILVAPEK 152

Query: 187 FKVW-GKGLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQVSPYDDPIDDEFKLLIPLNSTV 246
              W    L QWL F  +  LTIQG+G ++GRG  WW +  Y                  
Sbjct: 153 IGSWPNSSLFQWLNFKWVSHLTIQGSGTLNGRGYNWWNLDTY------------------ 212

Query: 247 QEKPQAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNCIGVLVHDF 306
               Q   R++ +   P +KPTALRFY S NVTV  I+I NSP CHLKFD+  GV V++ 
Sbjct: 213 ----QTQTRNKYI---PPMKPTALRFYSSNNVTVRDISIVNSPLCHLKFDDSDGVKVNNI 272

Query: 307 RVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWSIFVNLKRSGEIIKN 366
            +SSP NS NTDGIHLQN+++V I  S ++C                             
Sbjct: 273 TISSPENSPNTDGIHLQNTRNVEIQHSNIAC----------------------------- 332

Query: 367 FLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILCDDCISIQTGCSNVY 426
                                                         DDC+SIQTG SNV+
Sbjct: 333 ---------------------------------------------GDDCVSIQTGSSNVH 392

Query: 427 IHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGLGFVQGVLF 486
           IH++NCGPGHGISIG LGKD + ACVS+I V D+ + NT+ GVRIKTWQGGLG V+ + F
Sbjct: 393 IHHINCGPGHGISIGGLGKDKSVACVSDIIVEDISIQNTLAGVRIKTWQGGLGVVKNLTF 440

Query: 487 SNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVKPVHFACSDNLPC 546
           SNIQV +VK+PIVIDQ+YCDK+KC NQT AV++SG+ Y  I G++TV+PV  ACS+N+PC
Sbjct: 453 SNIQVKDVKVPIVIDQYYCDKSKCKNQTRAVSISGVKYNNIVGSFTVQPVRIACSNNVPC 440

Query: 547 TDISLTAIELKPL-----QELYHLYGAFCWQTFGELRTPTVP 580
            D+ L  I L+P       + +    A CW ++G+ + P VP
Sbjct: 513 MDVDLMDIRLRPSGGIRGLQTHQQQQALCWNSYGKTQGPLVP 440

BLAST of ClCG05G025420 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 346.3 bits (887), Expect = 5.0e-95
Identity = 213/537 (39.66%), Postives = 277/537 (51.58%), Query Frame = 0

Query: 63  HKGNHHGSASKTKPPPSRNTPQPLP---PPVTKPKPDVPRSPPPQKGGHSS--MFNVLDF 122
           HKG  HG      PPP    P PL    PP   P    P SP P  G   S  +F+V  F
Sbjct: 32  HKG-RHGVTHPLPPPP----PPPLETANPPDQVPSDPYP-SPDPAPGDSDSGCVFDVTSF 91

Query: 123 GAKGDGETDDTKAFQDTWTASCKVAASTFIVPSKYVFLVGPISFSGPYCQPDIVFQLDGT 182
           GA GDG  DDT AFQD W A+C V +   + P   VF +    FSGP C+P +VFQLDG 
Sbjct: 92  GAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGP-CKPGLVFQLDGV 151

Query: 183 IIAPTDFKVWGK--GLLQWLQFTKLMGLTIQGNGIIDGRGSAWWQVSPYDDPIDDEFKLL 242
           ++ P   + W +     QWL F +L G T  G G ++G G  WW                
Sbjct: 152 LMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWD--------------- 211

Query: 243 IPLNSTVQEKP-QAPARSELVGKMPRIKPTALRFYGSFNVTVTGITIQNSPQCHLKFDNC 302
           +P       KP + P  S   G  P   PT +RF+ S N+ V G+ IQNSPQ H+KFD C
Sbjct: 212 LPC------KPHRGPDGSSSSG--PCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGC 271

Query: 303 IGVLVHDFRVSSPGNSLNTDGIHLQNSKDVLIYSSTLSCVIKTIGKLNDINQWSIFVNLK 362
            GVL+++ ++SSP  S NTDGIHL N++ V IY+S +S                      
Sbjct: 272 QGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVS---------------------- 331

Query: 363 RSGEIIKNFLATRGFIPPSPSPLSSNQTQDVRQIASSQSSPISLLSFMIFNILCDDCISI 422
            +G                                                   DDCISI
Sbjct: 332 -NG---------------------------------------------------DDCISI 391

Query: 423 QTGCSNVYIHNVNCGPGHGISIGSLGKDNTKACVSNITVRDVIMHNTMNGVRIKTWQGGL 482
            TGCS+V I  V CGP HGISIGSLG  N++ACVSNITVR+ ++ ++ NG+R+KTWQGG 
Sbjct: 392 GTGCSDVDIQGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGT 451

Query: 483 GFVQGVLFSNIQVSEVKIPIVIDQFYCDKAKCSNQTSAVALSGINYERIRGTYTVK--PV 542
           G V  +LF NIQ+  V   I++DQ+YC    C N+TSAV +  + Y  I+GTY V+  P+
Sbjct: 452 GSVSNLLFENIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPI 463

Query: 543 HFACSDNLPCTDISLTAIELKPLQELYHLYGAFCWQTFGELRTPTVPPIDCLQIGKP 590
           HFACSD + CT+I+++ +EL P +E   +   FCW  +G+  T T+PPIDCL  G P
Sbjct: 512 HFACSDTVACTNITMSEVELLP-EEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSP 463

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893387.16.6e-29183.03polygalacturonase At1g48100-like isoform X2 [Benincasa hispida][more]
XP_038893386.14.7e-28982.34polygalacturonase At1g48100-like isoform X1 [Benincasa hispida][more]
XP_008446197.16.2e-28982.89PREDICTED: polygalacturonase At1g48100-like [Cucumis melo] >XP_016900181.1 PREDI... [more]
XP_004135250.11.9e-28581.89polygalacturonase At1g48100 [Cucumis sativus] >KGN51797.1 hypothetical protein C... [more]
KAA0034307.14.6e-28478.83polygalacturonase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q949Z17.0e-9439.66Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ53.5e-6933.76Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
Q7M1E79.6e-6734.68Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P432122.0e-6432.96Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
P353362.0e-6434.45Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BFB43.0e-28982.89polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103489001 PE=3 SV... [more]
A0A5D3CUP53.0e-28982.89Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001... [more]
E5GBI63.0e-28982.89Polygalacturonase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A0A0KTN99.0e-28681.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G601470 PE=3 SV=1[more]
A0A5A7SYF12.2e-28478.83Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003... [more]
Match NameE-valueIdentityDescription
AT1G60590.11.9e-19555.77Pectin lyase-like superfamily protein [more]
AT1G10640.11.6e-19456.74Pectin lyase-like superfamily protein [more]
AT5G14650.11.6e-14152.21Pectin lyase-like superfamily protein [more]
AT3G26610.18.5e-10340.04Pectin lyase-like superfamily protein [more]
AT1G48100.15.0e-9539.66Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 448..469
e-value: 4.3
score: 16.3
coord: 477..498
e-value: 7400.0
score: 1.0
coord: 418..438
e-value: 4000.0
score: 2.9
coord: 271..320
e-value: 220.0
score: 10.6
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 101..585
e-value: 2.2E-111
score: 374.7
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 390..552
e-value: 2.4E-46
score: 158.3
coord: 141..222
e-value: 2.3E-8
score: 33.5
coord: 260..331
e-value: 2.0E-12
score: 46.9
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 425..438
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 78..102
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..118
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..70
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 408..591
coord: 8..333
NoneNo IPR availablePANTHERPTHR31375:SF192PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 408..591
NoneNo IPR availablePANTHERPTHR31375:SF192PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 8..333
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 122..553

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG05G025420.2ClCG05G025420.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity