ClCG05G022715 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG05G022715
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	Homocysteine S-methyltransferase family protein
Location	CG_Chr05: 34583574 .. 34587520 (-)
RNA-Seq Expression	ClCG05G022715
Synteny	ClCG05G022715

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAAGGGTAGAATGAGAATTTTAGAAAGTTGAATGGGCTTGAACCCATCAATTTAAGAAAGAACAATGGAAATGGATTTGGGCTTCAATTCTCCCACCAACCTAGAGGGCTACCAAAACCATTGGCGCGCATGACTTTACTAGAAAGCGATAAACCTACGTCGGTCCGGAGAATCTGAGCGAAAAGGCGTCCATGTTCAGAGACAGAGCGACATTCTTTCATATTCATATTGACTTTGTCGCTTAATTCCGTCTTCCTTGGTCATCGCCGGTAATTGCGTATTGATTTTCAAGGGAACGAAGAGGAAGAAGAAGAAGAGAAACAGCGAGACAGACAGAACGAGGAGATGGGGATGAAGAAGGCGGCCACATTGTTGGATGATCTGTTACAGAACGCTGGAGGTTGTGGGGTGATTGATGGAGGTTTCGCCACGCAGCTTGAGAAACACGGCGCTATCATTAACGATCCACTATGGAGTGCTGTTTGCCTCATTAATGATCCTGACCTAATCAAGAAGGTCCTCTCTTACAGTTGTTGTTGTTTTCTCCTCCTTTGTTCCGTGCATCACGTTCTCATTCTCATCGGTGTTTATTGATCGGATTAAATGTGCAGGTTCATTTGGAGTACTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCATCTTACCAGGTTTGTAATGCGAATGGACTGAGCTTTCTTTATTCTTTTTTCCACTGTATACGTGAAAGTTTTTTGTACATTGCACTCTGTGACCGCTTTGGAGTACCTTACTTTTAAATGCTGTAATGTTGTTTGTTTAAAACTTTATGATTCAAATTGGATGTTTTCTAATGCTGATGAACAGAATGAATGAGGATCAAACTGATCACTCCTCTTGAATTGAAATTAAAGTTCAAAATGACTATCCTGTCGTGAAACGATGAAGCAAAAGTGAACCGCTGTTAAATGAATTGGATTAAAGTGCTTCTCATGGAGTAACATTTCTGGACATTCAAGTTGTTATAATTAGTAGACCCAAATTATGCTACATTACAGTTCAGTTTGCAACCGAATAAGATAAGTGACAATATACAGGCAACACCACAGCATTCACTTGTCAAAAGTGCACGAATGGCATTTAAAGTGAAAGTTTCCAATGTTAAACGTTGGAGAATGAAGCGATCAGTTCATCAGCTTGATTTAATGGCATTTAAACTGATATTTAGTTTGTCATGCTATATGCCCATCACAATACAAAAGAGCTTCATTTTGCCATTGAAAATGAAATTCAATTTTGTATAATTCAGGATTTGTATAGATCAATATATATTGAATTTATATGCCTTTTTTCAGTTTTCACAACTTTATTATACCATATCAGAATTCTCTATATTTTATTGACCCGATATAAAATTGGAGTGTGAAATATGACATCAGTTTGTGCCTTATAGTTTCTTAAGAATTCCAATAATCTTAAGGAATCAGATCGAAGTATTTTTCTTGCTTTATTTCCTCAGAAACTTTTTATAGAAATAATTCCTTCTGTTAGAGATTGTGAACATAATTCCAATAAATTGGTATTCAAGCCAGTTAATTACATCTCTTGTCACACATTTTATTCTATTATAGGCGACCATTCCTGGGTTTATATCAAAGGGGCTGTCTGTGGAAGAAGGGGAGTTGCTCTTAGAAAAGAGTGTTAAATTGGCCATCGAAGCTCGTGATAGTTTCTGGGATTCTGTGAAGAGTATCCCAGGGCATAAGTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCCTATCTTGCTGATGGCTCAGAGTATAGGTGAATCATACATATGAAATATTGAAATAAGGAGTTTGTAAATATATGGCATTGACAATCTGAACTAAACGTATTACACCCCCCCCCAGTGGGCATTATGGGCCAGATGTGAATTTGGATAAGCTGAAGGATTTTCATCGACGCAGATTGCAGATTCTTGTTGATGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTTGAAGCCCAGGTTTGTTTTATGTCAACGCTCATATCATCTAAATTGTGATTTAATGATATACACACTCGTACTGCCGCAGTGATGATACCACTGTGAATTATGTTGTTAAGCTTTGGTCGAGTCATAAAGTATTTGAGGAAACAGCTTACCTTATATTTTATTATCTGTTCTGAATTTATGAGTTGTTCATTTCAAGGGATCGAGGTAATTTGATGATCGCCATGGCGTTAATGGGGGATCCATTCATTATTAGGTTTTAGTTTATGAAGTGTCTTCATGATCAAAATTGTTTTATAATAAAAATTTGATTTAAATCCTGTTCAAGTCCCTTAATTTAAAAGTGATCCCATTTTACATTTCCAGATACTCTATTTTAATTCCTAAACCTGAATCCTGATTTAGTTAGTCTTTGTTATGAAATGGAAGAAAAATTGTTGAATGTTTCTGATTCTTGTCATGAGCATGTCAGGACACTTGTTTCAATTGTTCTTGCATTTTGCTTGGTTTTCAGTCCTCTCGATGATCGTGTTTCCAACCAATCTTACCTTTGTTTCCCCCACTCTGGTCTAAAAAAAGTAGGCATGTGTGGAGTTGCTCGAAGAAGAAAACATCCAGATTCCATCTTGGATCTGCTTTAGCTCCGTAGATGGTGAGAATGCACCATCTGGAGAGAGCTTCAAGAAGTGCCTCCATGCGATTAATAAAAGTGACAAAGTAAATGCGGTTGGCATAAACTGTACACCTCCTCATTTTATTGAAGCTCTGATTACAAAGTTCAAAGAGGTAGGAACTAAATCTCTATAGTTGATTCGATATTGTTTTCCTGTATGATGGTTTTAAAACCGAAGTGTTCACTACAGTTAACAAACAAGCACATTGTTGTCTATCCCAATAGTGGGGAGGTATGGGATGGCAGATCCAAGAAATGGCTGGTGAGTCAACTTATTCTTCCCTCCCCCACTATGATATTTCATGACTTGTGGGCCTATATTGTGATATTTCCATTACTAATTTAGTGTTTTGGTTTTGGTAGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCATTTAGTTCAAGATGGCGCGATTTGGGAGCAACATTCATTGGAGGCTGTTGTCGTACGACACCATCTACCATCCGTTCTATATCAAAGGTTTTGAAAGAAAGCTCTCATTAGTGACAATTGATGAGTTGACTGTCTGTAGTTGGATGGGATTGGTGACTGAACGGAAAGATTATACTAAAGCGAGGCAAGTTGGTCCGTAACTTCAAAATACTTTCTTTGGAATAATTTTTAACCTTTTTAAATTTCAATTTTAATTTTTTTTGTAACTTCTAGAGTGAGGTGTTACAGTTTGTACTCAAGGCATACTTGTTGGCTTTATAGACTTTTATTGTATCCAACTCGAAAGGCTAGTTAAATAAAATAGGCATTTTCTCCTATTTGTGATATTCAAATAAATTGGAAAAGAAAATTATTACATTTTTAGAGCATATTTTATATTTTATTTCAAGGAATGAAAAAAATCTTAGAGAGTGGGTCAATCAGGACAATGCAAACTTTGGATGCTGAGGCAGAACTTGGAAGTTTATGAGCAGGAAAATAAGAACCGACAGAATTCAAATCTCTTACTGGATTAGGAAATCTTTATGTATCAGAATTTTTCCCAATATTCCCAATCTTAGTTGCTTATAACATACTTGCAAGTGTGCATATATAAAATCTCGACTGATTTGATCAGTTGAGCTGGGCTGAGAAAATCTTCTTTTTTTCTGGGAAAAGATAAAAAGTGGAGAAACAATTGTTTATCCTCAACGTAGACTGAGAAGAAGAAGTCGAAAAGTATGGGATTAGACAGAAGGCCATGGATAATGCAATTTGGGATGGGGGACCCTAAACAAGATCTCAACTTTTAG

mRNA sequence

ATGGTAAGGAATGAGAATTTTAGAAAGTTGAATGGGCTTGAACCCATCAATTTAAGAAAGAACAATGGAAATGGATTTGGGCTTCAATTCTCCCACCAACCTAGAGGGCTACCAAAACCATTGGCGCGCATGACTTTACTAGAAAGCGATAAACCTACGTCGGAAGAAGAAGAAGAGAAACAGCGAGACAGACAGAACGAGGAGATGGGGATGAAGAAGGCGGCCACATTGTTGGATGATCTGTTACAGAACGCTGGAGGTTGTGGGGTGATTGATGGAGGTTTCGCCACGCAGCTTGAGAAACACGGCGCTATCATTAACGATCCACTATGGAGTGCTGTTTGCCTCATTAATGATCCTGACCTAATCAAGAAGTTGTTGTTGTTTTCTCCTCCTTTGTTCCGTGCATCACGTTCTCATTCTCATCGGTGTTTATTGATCGGATTAAATGTGCAGGTTCATTTGGAGTACTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCATCTTACCAGGCGACCATTCCTGGGTTTATATCAAAGGGGCTGTCTGTGGAAGAAGGGGAGTTGCTCTTAGAAAAGAGTGTTAAATTGGCCATCGAAGCTCGTGATAGTTTCTGGGATTCTGTGAAGAGTATCCCAGGGCATAAGTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCCTATCTTGCTGATGGCTCAGAGTATAGATTGCAGATTCTTGTTGATGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTTGAAGCCCAGGCATGTGTGGAGTTGCTCGAAGAAGAAAACATCCAGATTCCATCTTGGATCTGCTTTAGCTCCGTAGATGGTGAGAATGCACCATCTGGAGAGAGCTTCAAGAAGTGCCTCCATGCGATTAATAAAAGTGACAAAGTAAATGCGGTTGGCATAAACTGTACACCTCCTCATTTTATTGAAGCTCTGATTACAAAGTTCAAAGAGTTAACAAACAAGCACATTGTTGTCTATCCCAATAGTGGGGAGGTATGGGATGGCAGATCCAAGAAATGGCTGGTGAGTCAACTTATTCTTCCCTCCCCCACTATGATATTTCATGACTTGTGGGCCTATATTTGTTTTGGTTTTGGTAGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCATTTAGTTCAAGATGGCGCGATTTGGGAGCAACATTCATTGGAGGCTGTTGTCGTACGACACCATCTACCATCCGTTCTATATCAAAGAAAGCTCTCATTAGTGACAATTGATGAGTTGACTGTCTGTAGTTGGATGGGATTGGTGACTGAACGGAAAGATTATACTAAAGCGAGGCAAACTGAGAAGAAGAAGTCGAAAAGTATGGGATTAGACAGAAGGCCATGGATAATGCAATTTGGGATGGGGGACCCTAAACAAGATCTCAACTTTTAG

Coding sequence (CDS)

ATGGTAAGGAATGAGAATTTTAGAAAGTTGAATGGGCTTGAACCCATCAATTTAAGAAAGAACAATGGAAATGGATTTGGGCTTCAATTCTCCCACCAACCTAGAGGGCTACCAAAACCATTGGCGCGCATGACTTTACTAGAAAGCGATAAACCTACGTCGGAAGAAGAAGAAGAGAAACAGCGAGACAGACAGAACGAGGAGATGGGGATGAAGAAGGCGGCCACATTGTTGGATGATCTGTTACAGAACGCTGGAGGTTGTGGGGTGATTGATGGAGGTTTCGCCACGCAGCTTGAGAAACACGGCGCTATCATTAACGATCCACTATGGAGTGCTGTTTGCCTCATTAATGATCCTGACCTAATCAAGAAGTTGTTGTTGTTTTCTCCTCCTTTGTTCCGTGCATCACGTTCTCATTCTCATCGGTGTTTATTGATCGGATTAAATGTGCAGGTTCATTTGGAGTACTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCATCTTACCAGGCGACCATTCCTGGGTTTATATCAAAGGGGCTGTCTGTGGAAGAAGGGGAGTTGCTCTTAGAAAAGAGTGTTAAATTGGCCATCGAAGCTCGTGATAGTTTCTGGGATTCTGTGAAGAGTATCCCAGGGCATAAGTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCCTATCTTGCTGATGGCTCAGAGTATAGATTGCAGATTCTTGTTGATGCAAGCCCAGATTTGCTTGCATTTGAGACTATTCCCAATAAACTTGAAGCCCAGGCATGTGTGGAGTTGCTCGAAGAAGAAAACATCCAGATTCCATCTTGGATCTGCTTTAGCTCCGTAGATGGTGAGAATGCACCATCTGGAGAGAGCTTCAAGAAGTGCCTCCATGCGATTAATAAAAGTGACAAAGTAAATGCGGTTGGCATAAACTGTACACCTCCTCATTTTATTGAAGCTCTGATTACAAAGTTCAAAGAGTTAACAAACAAGCACATTGTTGTCTATCCCAATAGTGGGGAGGTATGGGATGGCAGATCCAAGAAATGGCTGGTGAGTCAACTTATTCTTCCCTCCCCCACTATGATATTTCATGACTTGTGGGCCTATATTTGTTTTGGTTTTGGTAGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCATTTAGTTCAAGATGGCGCGATTTGGGAGCAACATTCATTGGAGGCTGTTGTCGTACGACACCATCTACCATCCGTTCTATATCAAAGAAAGCTCTCATTAGTGACAATTGATGAGTTGACTGTCTGTAGTTGGATGGGATTGGTGACTGAACGGAAAGATTATACTAAAGCGAGGCAAACTGAGAAGAAGAAGTCGAAAAGTATGGGATTAGACAGAAGGCCATGGATAATGCAATTTGGGATGGGGGACCCTAAACAAGATCTCAACTTTTAG

Protein sequence

MVRNENFRKLNGLEPINLRKNNGNGFGLQFSHQPRGLPKPLARMTLLESDKPTSEEEEEKQRDRQNEEMGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLLFSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEYRLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGENAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLVSQLILPSPTMIFHDLWAYICFGFGSHQIVLVMTNLNHLVQDGAIWEQHSLEAVVVRHHLPSVLYQRKLSLVTIDELTVCSWMGLVTERKDYTKARQTEKKKSKSMGLDRRPWIMQFGMGDPKQDLNF

Homology

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BLAST of ClCG05G022715 vs. NCBI nr
Match: TYK09109.1 (homocysteine S-methyltransferase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 497.7 bits (1280), Expect = 1.2e-136
Identity = 261/356 (73.31%), Postives = 275/356 (77.25%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           MG++KA TLLDDLL NAGGC VIDGG ATQLEKHGA+ NDPLWSAVCLINDPDLIKK   
Sbjct: 1   MGIEKATTLLDDLLHNAGGCAVIDGGLATQLEKHGAVFNDPLWSAVCLINDPDLIKK--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VHLEYLEAGADILV+SSYQATIPGFISKGLSVEEGE
Sbjct: 61  ------------------------VHLEYLEAGADILVTSSYQATIPGFISKGLSVEEGE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGA-YLADGSEYRLQILVDA 248
           LLLEKSVKLAIEARDSFWDSV  +       A+V   + +     L D    RL+ILVDA
Sbjct: 121 LLLEKSVKLAIEARDSFWDSVIGLWLQHPLEAMVPILLMAQSTDKLKDFHRRRLRILVDA 180

Query: 249 SPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGENAPSGESFKKCLHAINKS 308
           SPDLLAFETIPNKLEAQACVELLEEEN+QIPSWICFSSVDGENAPSGESF+KCL+AINKS
Sbjct: 181 SPDLLAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLYAINKS 240

Query: 309 DKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLVSQLILPSPTM 368
           DKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGR KKWL           
Sbjct: 241 DKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRFKKWL----------- 300

Query: 369 IFHDLWAYICFGFGSHQIVLVMTNLNHLVQDGAIWEQHSLEAVVVRHHLPSVLYQR 424
                    C GFGSHQIVLVMTN NHL+QDGA WEQHSLEAVVV+H LPS LYQR
Sbjct: 301 ---------CSGFGSHQIVLVMTNSNHLLQDGANWEQHSLEAVVVQHRLPSELYQR 309

[Close]

BLAST of ClCG05G022715 vs. NCBI nr
Match: XP_038893227.1 (homocysteine S-methyltransferase 1 [Benincasa hispida])

HSP 1 Score: 494.6 bits (1272), Expect = 9.8e-136
Identity = 255/324 (78.70%), Postives = 260/324 (80.25%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           M MKKA TLLDDLLQNAGGC VIDGGFATQLEKHGA+INDPLWSAVCLINDPDLIKK   
Sbjct: 1   MVMKKATTLLDDLLQNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPDLIKK--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VHLEYL+AGADILVSSSYQATIPGFISKGLSVEEGE
Sbjct: 61  ------------------------VHLEYLKAGADILVSSSYQATIPGFISKGLSVEEGE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY--------- 248
           LLLEKSVKLAIEARD FWDSVKSIPGHKY+RALVAASIGSYGAYLADGSEY         
Sbjct: 121 LLLEKSVKLAIEARDGFWDSVKSIPGHKYHRALVAASIGSYGAYLADGSEYSGHYGPDVN 180

Query: 249 ----------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE 308
                     RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSS+DGE
Sbjct: 181 VDKLKDFHRRRLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSIDGE 240

Query: 309 NAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 368
           NAPSGESF+KCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD
Sbjct: 241 NAPSGESFEKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 297

Query: 369 GRSKKWLVSQLILPSPTMIFHDLW 374
           GRSKKWL S          F   W
Sbjct: 301 GRSKKWLPSNCFGDDKFESFSSRW 297

[Close]

BLAST of ClCG05G022715 vs. NCBI nr
Match: KAA0034555.1 (homocysteine S-methyltransferase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 492.3 bits (1266), Expect = 4.9e-135
Identity = 264/376 (70.21%), Postives = 279/376 (74.20%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           MG++KA TLLDDLL NAGGC VIDGG ATQLEKHGA+ NDPLWSAVCLINDPDLIKK   
Sbjct: 1   MGIEKATTLLDDLLHNAGGCAVIDGGLATQLEKHGAVFNDPLWSAVCLINDPDLIKK--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VHLEYLEAGADILV+SSYQATIPGFISKGLSVEEGE
Sbjct: 61  ------------------------VHLEYLEAGADILVTSSYQATIPGFISKGLSVEEGE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY--------- 248
           LLLEKSVKLAIEARDSFWDSVK IPGH YNRALVAASIGSYGAYLADGSEY         
Sbjct: 121 LLLEKSVKLAIEARDSFWDSVKCIPGHNYNRALVAASIGSYGAYLADGSEYSGHYGPDVN 180

Query: 249 ----------RLQILVDASPDLLAFETIPNKLEAQAC-VELLEEENIQIPSWICF-SSVD 308
                     RL+ILVDASPDLLAFETIPNKLEAQ C + +L    +    W  +  ++ 
Sbjct: 181 VDKLKDFHRRRLRILVDASPDLLAFETIPNKLEAQVCFMPILYHSQL----WFNYRPTLT 240

Query: 309 GENAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEV 368
             NAPSGESF+KCL+AINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEV
Sbjct: 241 LANAPSGESFEKCLYAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEV 300

Query: 369 WDGRSKKWLVSQLILPSPTMIFHDLWAYICFGFGSHQIVLVMTNLNHLVQDGAIWEQHSL 424
           WDGR KKWL                    C GFGSHQIVLVMTN NHL+QDGA WEQHSL
Sbjct: 301 WDGRFKKWL--------------------CSGFGSHQIVLVMTNSNHLLQDGANWEQHSL 325

[Close]

BLAST of ClCG05G022715 vs. NCBI nr
Match: XP_004135092.1 (homocysteine S-methyltransferase 1 isoform X1 [Cucumis sativus] >KGN52080.1 hypothetical protein Csa_008676 [Cucumis sativus])

HSP 1 Score: 486.5 bits (1251), Expect = 2.7e-133
Identity = 250/309 (80.91%), Postives = 254/309 (82.20%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           MG+KKA TLLDD L NAGGC VIDGGFATQLEKHGA+INDPLWSAVCLINDP LIKK   
Sbjct: 1   MGIKKATTLLDDFLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPHLIKK--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE
Sbjct: 61  ------------------------VHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY--------- 248
           LLLEKSVKLAIEARDSFWDSVK IPGHKYNRALVAASIGSYGAYLADGSEY         
Sbjct: 121 LLLEKSVKLAIEARDSFWDSVKCIPGHKYNRALVAASIGSYGAYLADGSEYSGHYGPDVN 180

Query: 249 ----------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE 308
                     RLQI VDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE
Sbjct: 181 VDKLKDFHRRRLQIFVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE 240

Query: 309 NAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 359
           NAPSGESF+KCL+AINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD
Sbjct: 241 NAPSGESFEKCLYAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 282

[Close]

BLAST of ClCG05G022715 vs. NCBI nr
Match: XP_008446599.1 (PREDICTED: homocysteine S-methyltransferase 1 [Cucumis melo])

HSP 1 Score: 481.9 bits (1239), Expect = 6.6e-132
Identity = 246/309 (79.61%), Postives = 254/309 (82.20%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           MG++KA TLLDDLL NAGGC VIDGG ATQLEKHGA+ NDPLWSAVCLINDPDLIKK   
Sbjct: 1   MGIEKATTLLDDLLHNAGGCAVIDGGLATQLEKHGAVFNDPLWSAVCLINDPDLIKK--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VHLEYLEAGADILV+SSYQATIPGFISKGLSVEEGE
Sbjct: 61  ------------------------VHLEYLEAGADILVTSSYQATIPGFISKGLSVEEGE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY--------- 248
           LLLEKSVKLAIEARDSFWDSVK IPGH YNRALVAASIGSYGAYLADGSEY         
Sbjct: 121 LLLEKSVKLAIEARDSFWDSVKCIPGHNYNRALVAASIGSYGAYLADGSEYSGHYGPDVN 180

Query: 249 ----------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE 308
                     RL+ILVDASPDLLAFETIPNKLEAQACVELLEEEN+QIPSWICFSSVDGE
Sbjct: 181 VDKLKDFHRRRLRILVDASPDLLAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGE 240

Query: 309 NAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 359
           NAPSGESF+KCL+AINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD
Sbjct: 241 NAPSGESFEKCLYAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 282

[Close]

BLAST of ClCG05G022715 vs. ExPASy Swiss-Prot
Match: A4ZGQ8 (Homocysteine S-methyltransferase 1 OS=Brassica oleracea var. italica OX=36774 GN=HMT1 PE=1 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.2e-112
Identity = 202/324 (62.35%), Postives = 238/324 (73.46%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           MG++K + LL+DL++  GGC V+DGGFATQLE HGA INDPLWSAV LI DP+LIK+   
Sbjct: 1   MGLEKKSALLEDLIEKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKDPELIKR--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VH+EYLEAGAD++V+SSYQATIPGF+S+GLS+EE E
Sbjct: 61  ------------------------VHMEYLEAGADVVVTSSYQATIPGFLSRGLSMEESE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY--------- 248
            LL+KSVKLA+EARD FWD V    GH YNRALVAASIGSYGAYLADGSEY         
Sbjct: 121 SLLQKSVKLAVEARDRFWDKVSKTSGHSYNRALVAASIGSYGAYLADGSEYSGSYGEDVS 180

Query: 249 ----------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE 308
                     R+Q+LV+ASPDLLAFETIPNKLEAQACVELLEEEN+QIP+WICF+SVDGE
Sbjct: 181 LDKLKDFHRRRIQVLVEASPDLLAFETIPNKLEAQACVELLEEENVQIPAWICFTSVDGE 240

Query: 309 NAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 368
           NAPSGESF++CL  +NKS+ + AVGINC PP F++ LI KF +LT K IVVYPNSGEVWD
Sbjct: 241 NAPSGESFQECLETLNKSNNICAVGINCAPPQFMDNLIRKFSKLTQKAIVVYPNSGEVWD 297

Query: 369 GRSKKWLVSQLILPSPTMIFHDLW 374
           G++KKWL SQ    +   +F   W
Sbjct: 301 GKAKKWLPSQCFGDAEFEMFATKW 297

[Close]

BLAST of ClCG05G022715 vs. ExPASy Swiss-Prot
Match: Q9SDL7 (Homocysteine S-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HMT-1 PE=1 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 3.7e-109
Identity = 198/324 (61.11%), Postives = 234/324 (72.22%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           M ++K + LL+DL++  GGC V+DGGFATQLE HGA INDPLWSAV LI +P+LIK+   
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKR--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VH+EYLEAGADI+V+SSYQATIPGF+S+GLS+EE E
Sbjct: 61  ------------------------VHMEYLEAGADIVVTSSYQATIPGFLSRGLSIEESE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY--------- 248
            LL+KSV+LA+EARD FW+ V  + GH YNRALVAASIGSYGAYLADGSEY         
Sbjct: 121 SLLQKSVELAVEARDRFWEKVSKVSGHSYNRALVAASIGSYGAYLADGSEYSGHYGENVS 180

Query: 249 ----------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE 308
                     RLQ+LV+A PDLLAFETIPNKLEAQACVELLEEE +QIP+WICF+SVDGE
Sbjct: 181 LDKLKDFHRRRLQVLVEAGPDLLAFETIPNKLEAQACVELLEEEKVQIPAWICFTSVDGE 240

Query: 309 NAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 368
            APSGESF++CL  +NKS+ + AVGINC PP FIE LI KF +LT K IVVYPNSGEVWD
Sbjct: 241 KAPSGESFEECLEPLNKSNNIYAVGINCAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWD 297

Query: 369 GRSKKWLVSQLILPSPTMIFHDLW 374
           G++K+WL SQ        +F   W
Sbjct: 301 GKAKQWLPSQCFGDDEFEMFATKW 297

[Close]

BLAST of ClCG05G022715 vs. ExPASy Swiss-Prot
Match: Q9FUN0 (Homocysteine S-methyltransferase 1 OS=Zea mays OX=4577 GN=HMT-1 PE=2 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.6e-93
Identity = 177/299 (59.20%), Postives = 205/299 (68.56%), Query Frame = 0


Query: 77  LLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLLFSPPLFRA 136
           +L+DL+  AGGC VIDGGFATQLE  GA INDPLWSA CLI  P L+K            
Sbjct: 3   VLEDLVARAGGCAVIDGGFATQLEALGADINDPLWSAACLITRPHLVK------------ 62

Query: 137 SRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVK 196
                          +VH++YLEAGAD+++SSSYQATIPGFI++G+SV E E LL  SVK
Sbjct: 63  ---------------EVHMQYLEAGADVIISSSYQATIPGFIARGMSVAEAEDLLRTSVK 122

Query: 197 LAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY----------------- 256
           LA EARD FW S        YNRALVAASIGSYGAYLADGSEY                 
Sbjct: 123 LANEARDEFWKSTLRKSKPIYNRALVAASIGSYGAYLADGSEYSGSYGADITAEKLKDFH 182

Query: 257 --RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGENAPSGESF 316
             RLQ+L  A PDL+AFE IPN++EAQA VELLEEE +QIPSWICFSSVDG+N  SGESF
Sbjct: 183 RRRLQVLASAGPDLIAFEAIPNQMEAQALVELLEEEKVQIPSWICFSSVDGKNLCSGESF 242

Query: 317 KKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWL 357
             CL  +N S+KV  VG+NCTPP FIE +I +F++ T K I VYPNSGEVWDGR+K+WL
Sbjct: 243 ADCLKILNASEKVAVVGVNCTPPQFIEGIICEFRKQTKKAIAVYPNSGEVWDGRAKRWL 274

[Close]

BLAST of ClCG05G022715 vs. ExPASy Swiss-Prot
Match: Q8LAX0 (Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 SV=2)

HSP 1 Score: 278.1 bits (710), Expect = 1.9e-73
Identity = 147/315 (46.67%), Postives = 195/315 (61.90%), Query Frame = 0


Query: 72  KKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLLFSP 131
           ++ ++L+ D L+  GG  V+DGGFAT+L++HGA INDPLWSA CLI  P L+ K      
Sbjct: 7   EETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTK------ 66

Query: 132 PLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGELLL 191
                                VHL+YLE+GA+I++++SYQATI GF++KGLSV E E LL
Sbjct: 67  ---------------------VHLDYLESGANIIITASYQATIQGFVAKGLSVGEAENLL 126

Query: 192 EKSVKLAIEARDSF--------WDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY---- 251
            +SV++  EAR+ F        WD   +    +    LVAAS+GSYGAYLADGSEY    
Sbjct: 127 RRSVEITYEAREIFYNRCTKGSWDFAYAGKASR-RPILVAASVGSYGAYLADGSEYSGIY 186

Query: 252 ---------------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFS 311
                          R+QIL  +  DL+AFETIPNKLEA+A  +LLEEE+I IP+W  F+
Sbjct: 187 GDSVSKETLKDFHRRRVQILAKSGADLIAFETIPNKLEAEAYADLLEEEDIDIPAWFSFT 246

Query: 312 SVDGENAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNS 360
           S DG + P G+S  +C    +    V A+GINCT P +I ALI   +++T K IVVYPNS
Sbjct: 247 SKDGVSVPRGDSVVECAKVADSCKNVVAIGINCTAPRYIHALIISLRQMTRKPIVVYPNS 293

[Close]

BLAST of ClCG05G022715 vs. ExPASy Swiss-Prot
Match: Q9M1W4 (Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.6e-72
Identity = 146/303 (48.18%), Postives = 188/303 (62.05%), Query Frame = 0


Query: 78  LDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLLFSPPLFRAS 137
           + D L+  GG  VIDGG AT+ E+HGA +NDPLWSA CL+  P LI              
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIH------------- 68

Query: 138 RSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKL 197
                          VHL+YLEAGADI+ S+SYQATI GF +KG S EE E LL+KSV++
Sbjct: 69  --------------TVHLDYLEAGADIISSASYQATIQGFEAKGFSREESESLLKKSVEI 128

Query: 198 AIEARDSFWDSVKSIPGH-----KYNRALVAASIGSYGAYLADGSEY------------- 257
           A EAR+S++D   +         K    LVAAS+GSYGAYLADGSEY             
Sbjct: 129 ATEARNSYYDKCGTSSSMDDKILKKRPILVAASVGSYGAYLADGSEYSGIYGDSITLEKL 188

Query: 258 ------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGENAPS 317
                 RLQ+L ++  DL+AFETIPNK+EAQA  +LLEE +++IP W  F+S DG N  S
Sbjct: 189 KDFHRRRLQVLAESGADLIAFETIPNKIEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVS 248

Query: 318 GESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSK 357
           G+S K+C+      +KV AVGINCTPP FIE L+ + +++T+K I+VYPNSGE +D   K
Sbjct: 249 GDSIKECISIAENCEKVVAVGINCTPPRFIEGLVLEIEKVTSKPILVYPNSGESYDADRK 284

[Close]

BLAST of ClCG05G022715 vs. ExPASy TrEMBL
Match: A0A5D3CFM3 (Homocysteine S-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00990 PE=4 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 5.6e-137
Identity = 261/356 (73.31%), Postives = 275/356 (77.25%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           MG++KA TLLDDLL NAGGC VIDGG ATQLEKHGA+ NDPLWSAVCLINDPDLIKK   
Sbjct: 1   MGIEKATTLLDDLLHNAGGCAVIDGGLATQLEKHGAVFNDPLWSAVCLINDPDLIKK--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VHLEYLEAGADILV+SSYQATIPGFISKGLSVEEGE
Sbjct: 61  ------------------------VHLEYLEAGADILVTSSYQATIPGFISKGLSVEEGE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGA-YLADGSEYRLQILVDA 248
           LLLEKSVKLAIEARDSFWDSV  +       A+V   + +     L D    RL+ILVDA
Sbjct: 121 LLLEKSVKLAIEARDSFWDSVIGLWLQHPLEAMVPILLMAQSTDKLKDFHRRRLRILVDA 180

Query: 249 SPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGENAPSGESFKKCLHAINKS 308
           SPDLLAFETIPNKLEAQACVELLEEEN+QIPSWICFSSVDGENAPSGESF+KCL+AINKS
Sbjct: 181 SPDLLAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLYAINKS 240

Query: 309 DKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSKKWLVSQLILPSPTM 368
           DKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGR KKWL           
Sbjct: 241 DKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRFKKWL----------- 300

Query: 369 IFHDLWAYICFGFGSHQIVLVMTNLNHLVQDGAIWEQHSLEAVVVRHHLPSVLYQR 424
                    C GFGSHQIVLVMTN NHL+QDGA WEQHSLEAVVV+H LPS LYQR
Sbjct: 301 ---------CSGFGSHQIVLVMTNSNHLLQDGANWEQHSLEAVVVQHRLPSELYQR 309

[Close]

BLAST of ClCG05G022715 vs. ExPASy TrEMBL
Match: A0A5A7SZC2 (Homocysteine S-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G005700 PE=4 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 2.4e-135
Identity = 264/376 (70.21%), Postives = 279/376 (74.20%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           MG++KA TLLDDLL NAGGC VIDGG ATQLEKHGA+ NDPLWSAVCLINDPDLIKK   
Sbjct: 1   MGIEKATTLLDDLLHNAGGCAVIDGGLATQLEKHGAVFNDPLWSAVCLINDPDLIKK--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VHLEYLEAGADILV+SSYQATIPGFISKGLSVEEGE
Sbjct: 61  ------------------------VHLEYLEAGADILVTSSYQATIPGFISKGLSVEEGE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY--------- 248
           LLLEKSVKLAIEARDSFWDSVK IPGH YNRALVAASIGSYGAYLADGSEY         
Sbjct: 121 LLLEKSVKLAIEARDSFWDSVKCIPGHNYNRALVAASIGSYGAYLADGSEYSGHYGPDVN 180

Query: 249 ----------RLQILVDASPDLLAFETIPNKLEAQAC-VELLEEENIQIPSWICF-SSVD 308
                     RL+ILVDASPDLLAFETIPNKLEAQ C + +L    +    W  +  ++ 
Sbjct: 181 VDKLKDFHRRRLRILVDASPDLLAFETIPNKLEAQVCFMPILYHSQL----WFNYRPTLT 240

Query: 309 GENAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEV 368
             NAPSGESF+KCL+AINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEV
Sbjct: 241 LANAPSGESFEKCLYAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEV 300

Query: 369 WDGRSKKWLVSQLILPSPTMIFHDLWAYICFGFGSHQIVLVMTNLNHLVQDGAIWEQHSL 424
           WDGR KKWL                    C GFGSHQIVLVMTN NHL+QDGA WEQHSL
Sbjct: 301 WDGRFKKWL--------------------CSGFGSHQIVLVMTNSNHLLQDGANWEQHSL 325

[Close]

BLAST of ClCG05G022715 vs. ExPASy TrEMBL
Match: A0A0A0KR42 (Hcy-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G609660 PE=4 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 1.3e-133
Identity = 250/309 (80.91%), Postives = 254/309 (82.20%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           MG+KKA TLLDD L NAGGC VIDGGFATQLEKHGA+INDPLWSAVCLINDP LIKK   
Sbjct: 1   MGIKKATTLLDDFLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPHLIKK--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE
Sbjct: 61  ------------------------VHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY--------- 248
           LLLEKSVKLAIEARDSFWDSVK IPGHKYNRALVAASIGSYGAYLADGSEY         
Sbjct: 121 LLLEKSVKLAIEARDSFWDSVKCIPGHKYNRALVAASIGSYGAYLADGSEYSGHYGPDVN 180

Query: 249 ----------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE 308
                     RLQI VDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE
Sbjct: 181 VDKLKDFHRRRLQIFVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE 240

Query: 309 NAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 359
           NAPSGESF+KCL+AINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD
Sbjct: 241 NAPSGESFEKCLYAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 282

[Close]

BLAST of ClCG05G022715 vs. ExPASy TrEMBL
Match: A0A1S3BEY1 (homocysteine S-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103489284 PE=4 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 3.2e-132
Identity = 246/309 (79.61%), Postives = 254/309 (82.20%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           MG++KA TLLDDLL NAGGC VIDGG ATQLEKHGA+ NDPLWSAVCLINDPDLIKK   
Sbjct: 1   MGIEKATTLLDDLLHNAGGCAVIDGGLATQLEKHGAVFNDPLWSAVCLINDPDLIKK--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VHLEYLEAGADILV+SSYQATIPGFISKGLSVEEGE
Sbjct: 61  ------------------------VHLEYLEAGADILVTSSYQATIPGFISKGLSVEEGE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY--------- 248
           LLLEKSVKLAIEARDSFWDSVK IPGH YNRALVAASIGSYGAYLADGSEY         
Sbjct: 121 LLLEKSVKLAIEARDSFWDSVKCIPGHNYNRALVAASIGSYGAYLADGSEYSGHYGPDVN 180

Query: 249 ----------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE 308
                     RL+ILVDASPDLLAFETIPNKLEAQACVELLEEEN+QIPSWICFSSVDGE
Sbjct: 181 VDKLKDFHRRRLRILVDASPDLLAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGE 240

Query: 309 NAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 359
           NAPSGESF+KCL+AINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD
Sbjct: 241 NAPSGESFEKCLYAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 282

[Close]

BLAST of ClCG05G022715 vs. ExPASy TrEMBL
Match: A0A6J1D951 (homocysteine S-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC111018744 PE=4 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 4.8e-128
Identity = 239/324 (73.77%), Postives = 255/324 (78.70%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           M MKKA TLLDDL++NAGGC VIDGGFATQLEKHGA+INDPLWSAVCLI DPDLIKK   
Sbjct: 1   MVMKKATTLLDDLIENAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLIKDPDLIKK--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VHLEYLEAGADI+VSSSYQATIPGF+SKGLS EEGE
Sbjct: 61  ------------------------VHLEYLEAGADIVVSSSYQATIPGFLSKGLSTEEGE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY--------- 248
           LLLEKSVKLA+EARDSFW+SVKSIPGHKYNRALVAASIGSYGAYLADGSEY         
Sbjct: 121 LLLEKSVKLAVEARDSFWNSVKSIPGHKYNRALVAASIGSYGAYLADGSEYSGNYGPDVN 180

Query: 249 ----------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE 308
                     RLQ+LV+A PDLLAFETIPNKLEAQACVELLEEEN+QIPSWICFSSVDG+
Sbjct: 181 VDKLKDFHRRRLQVLVNACPDLLAFETIPNKLEAQACVELLEEENVQIPSWICFSSVDGK 240

Query: 309 NAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 368
           NAPSGESF++CL AINKS+KVNAVGINCTPPHFIEALI KFKELT+K IVVYPNSGEVWD
Sbjct: 241 NAPSGESFEECLRAINKSNKVNAVGINCTPPHFIEALICKFKELTDKPIVVYPNSGEVWD 297

Query: 369 GRSKKWLVSQLILPSPTMIFHDLW 374
           GR+KKWL S          F   W
Sbjct: 301 GRAKKWLPSNCFGDDKFESFSSRW 297

[Close]

BLAST of ClCG05G022715 vs. TAIR 10
Match: AT3G25900.1 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 396.7 bits (1018), Expect = 2.6e-110
Identity = 198/324 (61.11%), Postives = 234/324 (72.22%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           M ++K + LL+DL++  GGC V+DGGFATQLE HGA INDPLWSAV LI +P+LIK+   
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKR--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VH+EYLEAGADI+V+SSYQATIPGF+S+GLS+EE E
Sbjct: 61  ------------------------VHMEYLEAGADIVVTSSYQATIPGFLSRGLSIEESE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY--------- 248
            LL+KSV+LA+EARD FW+ V  + GH YNRALVAASIGSYGAYLADGSEY         
Sbjct: 121 SLLQKSVELAVEARDRFWEKVSKVSGHSYNRALVAASIGSYGAYLADGSEYSGHYGENVS 180

Query: 249 ----------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE 308
                     RLQ+LV+A PDLLAFETIPNKLEAQACVELLEEE +QIP+WICF+SVDGE
Sbjct: 181 LDKLKDFHRRRLQVLVEAGPDLLAFETIPNKLEAQACVELLEEEKVQIPAWICFTSVDGE 240

Query: 309 NAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 368
            APSGESF++CL  +NKS+ + AVGINC PP FIE LI KF +LT K IVVYPNSGEVWD
Sbjct: 241 KAPSGESFEECLEPLNKSNNIYAVGINCAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWD 297

Query: 369 GRSKKWLVSQLILPSPTMIFHDLW 374
           G++K+WL SQ        +F   W
Sbjct: 301 GKAKQWLPSQCFGDDEFEMFATKW 297

[Close]

BLAST of ClCG05G022715 vs. TAIR 10
Match: AT3G25900.3 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 355.1 bits (910), Expect = 8.7e-98
Identity = 184/324 (56.79%), Postives = 219/324 (67.59%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           M ++K + LL+DL++  GGC V+DGGFATQLE HGA INDPLWSAV LI +P+LIK+   
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKR--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VH+EYLEAGADI+V+SSYQATIPGF+S+GLS+EE E
Sbjct: 61  ------------------------VHMEYLEAGADIVVTSSYQATIPGFLSRGLSIEESE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY--------- 248
            LL+KSV+LA+EARD FW+ V  + GH YNRALVAASIGSYGAYLADGSEY         
Sbjct: 121 SLLQKSVELAVEARDRFWEKVSKVSGHSYNRALVAASIGSYGAYLADGSEYSGHYGENVS 180

Query: 249 ----------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE 308
                     RLQ+LV+A PDLLAFETIPNKLEAQACVELLEEE +QIP+WICF+SVDGE
Sbjct: 181 LDKLKDFHRRRLQVLVEAGPDLLAFETIPNKLEAQACVELLEEEKVQIPAWICFTSVDGE 240

Query: 309 NAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWD 368
            APSGESF++CL  +NKS+ + A                    LT K IVVYPNSGEVWD
Sbjct: 241 KAPSGESFEECLEPLNKSNNIYA--------------------LTKKAIVVYPNSGEVWD 277

Query: 369 GRSKKWLVSQLILPSPTMIFHDLW 374
           G++K+WL SQ        +F   W
Sbjct: 301 GKAKQWLPSQCFGDDEFEMFATKW 277

[Close]

BLAST of ClCG05G022715 vs. TAIR 10
Match: AT3G25900.2 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 350.9 bits (899), Expect = 1.6e-96
Identity = 176/287 (61.32%), Postives = 210/287 (73.17%), Query Frame = 0


Query: 69  MGMKKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLL 128
           M ++K + LL+DL++  GGC V+DGGFATQLE HGA INDPLWSAV LI +P+LIK+   
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKR--- 60

Query: 129 FSPPLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGE 188
                                   VH+EYLEAGADI+V+SSYQATIPGF+S+GLS+EE E
Sbjct: 61  ------------------------VHMEYLEAGADIVVTSSYQATIPGFLSRGLSIEESE 120

Query: 189 LLLEKSVKLAIEARDSFWDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY--------- 248
            LL+KSV+LA+EARD FW+ V  + GH YNRALVAASIGSYGAYLADGSEY         
Sbjct: 121 SLLQKSVELAVEARDRFWEKVSKVSGHSYNRALVAASIGSYGAYLADGSEYSGHYGENVS 180

Query: 249 ----------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGE 308
                     RLQ+LV+A PDLLAFETIPNKLEAQACVELLEEE +QIP+WICF+SVDGE
Sbjct: 181 LDKLKDFHRRRLQVLVEAGPDLLAFETIPNKLEAQACVELLEEEKVQIPAWICFTSVDGE 240

Query: 309 NAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNK 337
            APSGESF++CL  +NKS+ + AVGINC PP FIE LI KF + T++
Sbjct: 241 KAPSGESFEECLEPLNKSNNIYAVGINCAPPQFIENLIRKFAKATDE 260

[Close]

BLAST of ClCG05G022715 vs. TAIR 10
Match: AT3G22740.1 (homocysteine S-methyltransferase 3 )

HSP 1 Score: 278.1 bits (710), Expect = 1.3e-74
Identity = 147/315 (46.67%), Postives = 195/315 (61.90%), Query Frame = 0


Query: 72  KKAATLLDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLLFSP 131
           ++ ++L+ D L+  GG  V+DGGFAT+L++HGA INDPLWSA CLI  P L+ K      
Sbjct: 7   EETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTK------ 66

Query: 132 PLFRASRSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGELLL 191
                                VHL+YLE+GA+I++++SYQATI GF++KGLSV E E LL
Sbjct: 67  ---------------------VHLDYLESGANIIITASYQATIQGFVAKGLSVGEAENLL 126

Query: 192 EKSVKLAIEARDSF--------WDSVKSIPGHKYNRALVAASIGSYGAYLADGSEY---- 251
            +SV++  EAR+ F        WD   +    +    LVAAS+GSYGAYLADGSEY    
Sbjct: 127 RRSVEITYEAREIFYNRCTKGSWDFAYAGKASR-RPILVAASVGSYGAYLADGSEYSGIY 186

Query: 252 ---------------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFS 311
                          R+QIL  +  DL+AFETIPNKLEA+A  +LLEEE+I IP+W  F+
Sbjct: 187 GDSVSKETLKDFHRRRVQILAKSGADLIAFETIPNKLEAEAYADLLEEEDIDIPAWFSFT 246

Query: 312 SVDGENAPSGESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNS 360
           S DG + P G+S  +C    +    V A+GINCT P +I ALI   +++T K IVVYPNS
Sbjct: 247 SKDGVSVPRGDSVVECAKVADSCKNVVAIGINCTAPRYIHALIISLRQMTRKPIVVYPNS 293

[Close]

BLAST of ClCG05G022715 vs. TAIR 10
Match: AT3G63250.1 (homocysteine methyltransferase 2 )

HSP 1 Score: 275.0 bits (702), Expect = 1.1e-73
Identity = 146/303 (48.18%), Postives = 188/303 (62.05%), Query Frame = 0


Query: 78  LDDLLQNAGGCGVIDGGFATQLEKHGAIINDPLWSAVCLINDPDLIKKLLLFSPPLFRAS 137
           + D L+  GG  VIDGG AT+ E+HGA +NDPLWSA CL+  P LI              
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIH------------- 68

Query: 138 RSHSHRCLLIGLNVQVHLEYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKL 197
                          VHL+YLEAGADI+ S+SYQATI GF +KG S EE E LL+KSV++
Sbjct: 69  --------------TVHLDYLEAGADIISSASYQATIQGFEAKGFSREESESLLKKSVEI 128

Query: 198 AIEARDSFWDSVKSIPGH-----KYNRALVAASIGSYGAYLADGSEY------------- 257
           A EAR+S++D   +         K    LVAAS+GSYGAYLADGSEY             
Sbjct: 129 ATEARNSYYDKCGTSSSMDDKILKKRPILVAASVGSYGAYLADGSEYSGIYGDSITLEKL 188

Query: 258 ------RLQILVDASPDLLAFETIPNKLEAQACVELLEEENIQIPSWICFSSVDGENAPS 317
                 RLQ+L ++  DL+AFETIPNK+EAQA  +LLEE +++IP W  F+S DG N  S
Sbjct: 189 KDFHRRRLQVLAESGADLIAFETIPNKIEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVS 248

Query: 318 GESFKKCLHAINKSDKVNAVGINCTPPHFIEALITKFKELTNKHIVVYPNSGEVWDGRSK 357
           G+S K+C+      +KV AVGINCTPP FIE L+ + +++T+K I+VYPNSGE +D   K
Sbjct: 249 GDSIKECISIAENCEKVVAVGINCTPPRFIEGLVLEIEKVTSKPILVYPNSGESYDADRK 284

The following BLAST results are available for this feature:

BLAST of ClCG05G022715 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK09109.1	1.2e-136	73.31	homocysteine S-methyltransferase 1 [Cucumis melo var. makuwa]	[more]
XP_038893227.1	9.8e-136	78.70	homocysteine S-methyltransferase 1 [Benincasa hispida]	[more]
KAA0034555.1	4.9e-135	70.21	homocysteine S-methyltransferase 1 [Cucumis melo var. makuwa]	[more]
XP_004135092.1	2.7e-133	80.91	homocysteine S-methyltransferase 1 isoform X1 [Cucumis sativus] >KGN52080.1 hypo...	[more]
XP_008446599.1	6.6e-132	79.61	PREDICTED: homocysteine S-methyltransferase 1 [Cucumis melo]	[more]

BLAST of ClCG05G022715 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A4ZGQ8	1.2e-112	62.35	Homocysteine S-methyltransferase 1 OS=Brassica oleracea var. italica OX=36774 GN...	[more]
Q9SDL7	3.7e-109	61.11	Homocysteine S-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HMT-1 PE=1...	[more]
Q9FUN0	1.6e-93	59.20	Homocysteine S-methyltransferase 1 OS=Zea mays OX=4577 GN=HMT-1 PE=2 SV=1	[more]
Q8LAX0	1.9e-73	46.67	Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 ...	[more]
Q9M1W4	1.6e-72	48.18	Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1...	[more]

BLAST of ClCG05G022715 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CFM3	5.6e-137	73.31	Homocysteine S-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A5A7SZC2	2.4e-135	70.21	Homocysteine S-methyltransferase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A0A0KR42	1.3e-133	80.91	Hcy-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G609660...	[more]
A0A1S3BEY1	3.2e-132	79.61	homocysteine S-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103489284 PE=4 ...	[more]
A0A6J1D951	4.8e-128	73.77	homocysteine S-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC11101874...	[more]

BLAST of ClCG05G022715 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25900.1	2.6e-110	61.11	Homocysteine S-methyltransferase family protein	[more]
AT3G25900.3	8.7e-98	56.79	Homocysteine S-methyltransferase family protein	[more]
AT3G25900.2	1.6e-96	61.32	Homocysteine S-methyltransferase family protein	[more]
AT3G22740.1	1.3e-74	46.67	homocysteine S-methyltransferase 3	[more]
AT3G63250.1	1.1e-73	48.18	homocysteine methyltransferase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036589	Homocysteine-binding domain superfamily	GENE3D	3.20.20.330		coord: 76..369 e-value: 1.9E-65 score: 223.2
IPR036589	Homocysteine-binding domain superfamily	SUPERFAMILY	82282	Homocysteine S-methyltransferase	coord: 152..356
IPR036589	Homocysteine-binding domain superfamily	SUPERFAMILY	82282	Homocysteine S-methyltransferase	coord: 85..126
IPR003726	Homocysteine-binding domain	PFAM	PF02574	S-methyl_trans	coord: 90..356 e-value: 2.7E-48 score: 165.3
IPR003726	Homocysteine-binding domain	PROSITE	PS50970	HCY	coord: 77..392 score: 21.975372
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 49..68
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 48..68
None	No IPR available	PANTHER	PTHR46015:SF7	HOMOCYSTEINE S-METHYLTRANSFERASE 1	coord: 152..360 coord: 69..126
None	No IPR available	PANTHER	PTHR46015	ZGC:172121	coord: 152..360
None	No IPR available	PANTHER	PTHR46015	ZGC:172121	coord: 69..126

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG05G022715.1	ClCG05G022715.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009086	methionine biosynthetic process
biological_process	GO:0032259	methylation
biological_process	GO:0033528	S-methylmethionine cycle
molecular_function	GO:0047150	betaine-homocysteine S-methyltransferase activity
molecular_function	GO:0008898	S-adenosylmethionine-homocysteine S-methyltransferase activity
molecular_function	GO:0008270	zinc ion binding