ClCG05G008690 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG05G008690
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionArabinogalactan peptide 22
LocationCG_Chr05: 9402118 .. 9403254 (-)
RNA-Seq ExpressionClCG05G008690
SyntenyClCG05G008690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAGAGCAAAAGCATAAAAAAGAAGGAATCTGTAGAGAGAGAGAAAGAGAATGAAGAATCACATGGGTTTGTGTGGATTCCCAAAGCTTGCGGTTGAAACACAAACAGTATTAAACCCCAATCCTTCCATTTCCATCCTCCTCATTCAAAACCTTCAAATTTCTTGTTCTTCTATTTCTCTCTAGGTTCCTCTGTGGAAGAAGTGGCAATTGGATTGAATAAACGCCAAGGACAGAGAGAGAGACAGAGATGGCTGTTTTGAGGGCTTGTTTTGGAATCTATGCTGCTGTGATTATGGCTGTCTTTTATGTTGTTTCTTTGCCTGTGGCTGTATCTGCAGCTGAACATTCATCTTCTCCAGCTCCAGCTCCCACTAGTGATGGTTAGTTTTCTTTTCTTCCTGGTTTTCTCTTTGTTTTGTTTGTTTCTTGAGAAATTTTCTGGGAAAAAGACGGGAAATCCGAGAAAATGGCTTTGAAAATACTCTTCCCTTTAAATTCTAGATTCTAAGAAGCACCAATCAATTCAATTGGGTCTCCTTGAATTGGAAATGGGGTTTGGGGAATTTGGCTCCTTGTTTGAAAAGAAGCAAAATTTACTCCTTTTCTCCCATGCCATTTTTAGACATTTTTCTGTTAGTTTCTTGAGAGGGGACTGACAGGGAATATGAGAATGGCTTTGAAATCAACTCTCATCTTCAAATACAAAGGTGAATCTCCTTAAATTGAAAATGGGTTTCTGGTATTTGAAGAGAAGAGTTGATTTCAAAGGCAAATAGAGTTGTTGGAATTGAATCTCCTTCCTAAATTGGGAAAGTTGACTCCTTGTTTGAATAGAAAACAATATCTACTCTTTTTTCTTCCTCAAGTCAAGAAGAAGAAGCCACCATTTTAGGACATTTTCTTAAAGAAACCTTGCTTAGCTATTAATGGTGTTAAACTCCAAAAATAACATTAATTTGGGATTTCATATGTTGCAGGAACCACAATAGACCAAGGAATAGCATATGTTCTAATGCTGTTGGCTTTAGTGCTCACTTATATCATCCATTGATGCAACCTTTGAAAGCACCAAAAACACAGAAGAAGAATTAATAACATTCAATTCCAATTATGAATTGCATTATTTCAAAGTGTA

mRNA sequence

GCAGAGCAAAAGCATAAAAAAGAAGGAATCTGTAGAGAGAGAGAAAGAGAATGAAGAATCACATGGGTTTGTGTGGATTCCCAAAGCTTGCGGTTGAAACACAAACAGTATTAAACCCCAATCCTTCCATTTCCATCCTCCTCATTCAAAACCTTCAAATTTCTTGTTCTTCTATTTCTCTCTAGGTTCCTCTGTGGAAGAAGTGGCAATTGGATTGAATAAACGCCAAGGACAGAGAGAGAGACAGAGATGGCTGTTTTGAGGGCTTGTTTTGGAATCTATGCTGCTGTGATTATGGCTGTCTTTTATGTTGTTTCTTTGCCTGTGGCTGTATCTGCAGCTGAACATTCATCTTCTCCAGCTCCAGCTCCCACTAGTGATGGAACCACAATAGACCAAGGAATAGCATATGTTCTAATGCTGTTGGCTTTAGTGCTCACTTATATCATCCATTGATGCAACCTTTGAAAGCACCAAAAACACAGAAGAAGAATTAATAACATTCAATTCCAATTATGAATTGCATTATTTCAAAGTGTA

Coding sequence (CDS)

ATGGCTGTTTTGAGGGCTTGTTTTGGAATCTATGCTGCTGTGATTATGGCTGTCTTTTATGTTGTTTCTTTGCCTGTGGCTGTATCTGCAGCTGAACATTCATCTTCTCCAGCTCCAGCTCCCACTAGTGATGGAACCACAATAGACCAAGGAATAGCATATGTTCTAATGCTGTTGGCTTTAGTGCTCACTTATATCATCCATTGA

Protein sequence

MAVLRACFGIYAAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLLALVLTYIIH
Homology
BLAST of ClCG05G008690 vs. NCBI nr
Match: XP_038898060.1 (arabinogalactan protein 41-like [Benincasa hispida] >XP_038898069.1 arabinogalactan protein 41-like [Benincasa hispida])

HSP 1 Score: 105.9 bits (263), Expect = 1.4e-19
Identity = 61/68 (89.71%), Postives = 65/68 (95.59%), Query Frame = 0

Query: 1  MAVLRACFGIYAAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLLA 60
          MAVL+ACFGIYAAVI+AVFYVV+LP  VSAAE SSSPAPAPTSDGTTIDQGIAYVLML+A
Sbjct: 1  MAVLKACFGIYAAVIIAVFYVVTLP--VSAAEFSSSPAPAPTSDGTTIDQGIAYVLMLVA 60

Query: 61 LVLTYIIH 69
          LVLTYIIH
Sbjct: 61 LVLTYIIH 66

BLAST of ClCG05G008690 vs. NCBI nr
Match: TYK03143.1 (arabinogalactan peptide 22 [Cucumis melo var. makuwa])

HSP 1 Score: 98.6 bits (244), Expect = 2.2e-17
Identity = 59/69 (85.51%), Postives = 65/69 (94.20%), Query Frame = 0

Query: 1  MAVLRA-CFGIYAAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLL 60
          MAVLRA CFGIYAAV++AVFYV++LP  VS+AE SSSPAPAPTSDGTTIDQGIAYVLML+
Sbjct: 1  MAVLRASCFGIYAAVLIAVFYVLALP--VSSAELSSSPAPAPTSDGTTIDQGIAYVLMLV 60

Query: 61 ALVLTYIIH 69
          ALVLTYIIH
Sbjct: 61 ALVLTYIIH 67

BLAST of ClCG05G008690 vs. NCBI nr
Match: XP_022945187.1 (arabinogalactan peptide 22-like [Cucurbita moschata] >XP_023541894.1 arabinogalactan peptide 22-like [Cucurbita pepo subsp. pepo] >KAG7013292.1 Arabinogalactan peptide 22 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 97.8 bits (242), Expect = 3.8e-17
Identity = 57/68 (83.82%), Postives = 61/68 (89.71%), Query Frame = 0

Query: 1  MAVLRACFGIYAAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLLA 60
          MAVLRACFGIY AVI+A+FYVV LP  VS AEH+SSPAPAPTSDGT IDQGIAY+LMLLA
Sbjct: 1  MAVLRACFGIY-AVIIAIFYVVMLP--VSRAEHASSPAPAPTSDGTAIDQGIAYILMLLA 60

Query: 61 LVLTYIIH 69
          L LTYIIH
Sbjct: 61 LALTYIIH 65

BLAST of ClCG05G008690 vs. NCBI nr
Match: XP_008465391.1 (PREDICTED: arabinogalactan peptide 22 [Cucumis melo] >KAA0050462.1 arabinogalactan peptide 22 [Cucumis melo var. makuwa])

HSP 1 Score: 96.3 bits (238), Expect = 1.1e-16
Identity = 58/69 (84.06%), Postives = 65/69 (94.20%), Query Frame = 0

Query: 1  MAVLRA-CFGIYAAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLL 60
          MAVLRA CFGIYAAV++AVFYV++L  +VS+AE SSSPAPAPTSDGTTIDQGIAYVLML+
Sbjct: 1  MAVLRASCFGIYAAVLIAVFYVLAL--SVSSAELSSSPAPAPTSDGTTIDQGIAYVLMLV 60

Query: 61 ALVLTYIIH 69
          ALVLTYIIH
Sbjct: 61 ALVLTYIIH 67

BLAST of ClCG05G008690 vs. NCBI nr
Match: XP_022140891.1 (arabinogalactan peptide 22-like [Momordica charantia])

HSP 1 Score: 93.2 bits (230), Expect = 9.3e-16
Identity = 54/68 (79.41%), Postives = 60/68 (88.24%), Query Frame = 0

Query: 1  MAVLRACFGIYAAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLLA 60
          MAV RACFGIYA +I A+ +V+ LP  VS AEHSSSPAPAP+SDGTTIDQGIAY+LMLLA
Sbjct: 1  MAVSRACFGIYAGII-AILFVIILP--VSRAEHSSSPAPAPSSDGTTIDQGIAYILMLLA 60

Query: 61 LVLTYIIH 69
          LVLTYIIH
Sbjct: 61 LVLTYIIH 65

BLAST of ClCG05G008690 vs. ExPASy Swiss-Prot
Match: Q9FK16 (Arabinogalactan protein 22 OS=Arabidopsis thaliana OX=3702 GN=AGP22 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.1e-11
Identity = 37/54 (68.52%), Postives = 45/54 (83.33%), Query Frame = 0

Query: 15 IMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLLALVLTYIIH 69
          I+AVF ++S+ +   A  HSSSPAPAPTSDGT+IDQGIAYVLM++AL LTY IH
Sbjct: 10 ILAVFVIISVILLPIAQSHSSSPAPAPTSDGTSIDQGIAYVLMMVALALTYFIH 63

BLAST of ClCG05G008690 vs. ExPASy Swiss-Prot
Match: Q8L9T8 (Arabinogalactan protein 41 OS=Arabidopsis thaliana OX=3702 GN=AGP41 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 6.0e-10
Identity = 39/68 (57.35%), Postives = 53/68 (77.94%), Query Frame = 0

Query: 1  MAVLRACFGIYAAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLLA 60
          M+  R  FG+  + I+++ + + LP+A +    S++PAPAPTSDGTTIDQGIAYVLML+A
Sbjct: 1  MSGSRLFFGV--STIVSIIFAILLPMAHA---QSAAPAPAPTSDGTTIDQGIAYVLMLVA 60

Query: 61 LVLTYIIH 69
          LVLTY+IH
Sbjct: 61 LVLTYLIH 63

BLAST of ClCG05G008690 vs. ExPASy Swiss-Prot
Match: O82337 (Arabinogalactan protein 16 OS=Arabidopsis thaliana OX=3702 GN=AGP16 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 4.3e-08
Identity = 32/52 (61.54%), Postives = 40/52 (76.92%), Query Frame = 0

Query: 17 AVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLLALVLTYIIH 69
          A+F  V   +   A   S +PAPAPTSDGT+IDQGIAY+LM++ALVLTY+IH
Sbjct: 11 ALFSFVFAVILSLAGAQSLAPAPAPTSDGTSIDQGIAYLLMVVALVLTYLIH 62

BLAST of ClCG05G008690 vs. ExPASy Swiss-Prot
Match: Q9M373 (Arabinogalactan protein 20 OS=Arabidopsis thaliana OX=3702 GN=AGP20 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 4.3e-08
Identity = 31/57 (54.39%), Postives = 44/57 (77.19%), Query Frame = 0

Query: 12 AAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLLALVLTYIIH 69
          +  ++A+F  V   ++  A   S +PAP+PTSDGT+IDQGIAY+LM++ALVLTY+IH
Sbjct: 6  SVAVIALFAFVFAVISPFAGAQSLAPAPSPTSDGTSIDQGIAYLLMVVALVLTYLIH 62

BLAST of ClCG05G008690 vs. ExPASy TrEMBL
Match: A0A5D3BTZ5 (Arabinogalactan peptide 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11G00180 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.1e-17
Identity = 59/69 (85.51%), Postives = 65/69 (94.20%), Query Frame = 0

Query: 1  MAVLRA-CFGIYAAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLL 60
          MAVLRA CFGIYAAV++AVFYV++LP  VS+AE SSSPAPAPTSDGTTIDQGIAYVLML+
Sbjct: 1  MAVLRASCFGIYAAVLIAVFYVLALP--VSSAELSSSPAPAPTSDGTTIDQGIAYVLMLV 60

Query: 61 ALVLTYIIH 69
          ALVLTYIIH
Sbjct: 61 ALVLTYIIH 67

BLAST of ClCG05G008690 vs. ExPASy TrEMBL
Match: A0A6J1G081 (arabinogalactan peptide 22-like OS=Cucurbita moschata OX=3662 GN=LOC111449506 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.8e-17
Identity = 57/68 (83.82%), Postives = 61/68 (89.71%), Query Frame = 0

Query: 1  MAVLRACFGIYAAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLLA 60
          MAVLRACFGIY AVI+A+FYVV LP  VS AEH+SSPAPAPTSDGT IDQGIAY+LMLLA
Sbjct: 1  MAVLRACFGIY-AVIIAIFYVVMLP--VSRAEHASSPAPAPTSDGTAIDQGIAYILMLLA 60

Query: 61 LVLTYIIH 69
          L LTYIIH
Sbjct: 61 LALTYIIH 65

BLAST of ClCG05G008690 vs. ExPASy TrEMBL
Match: A0A5A7U3P4 (Arabinogalactan peptide 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G00750 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 5.3e-17
Identity = 58/69 (84.06%), Postives = 65/69 (94.20%), Query Frame = 0

Query: 1  MAVLRA-CFGIYAAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLL 60
          MAVLRA CFGIYAAV++AVFYV++L  +VS+AE SSSPAPAPTSDGTTIDQGIAYVLML+
Sbjct: 1  MAVLRASCFGIYAAVLIAVFYVLAL--SVSSAELSSSPAPAPTSDGTTIDQGIAYVLMLV 60

Query: 61 ALVLTYIIH 69
          ALVLTYIIH
Sbjct: 61 ALVLTYIIH 67

BLAST of ClCG05G008690 vs. ExPASy TrEMBL
Match: A0A1S3CNS5 (arabinogalactan peptide 22 OS=Cucumis melo OX=3656 GN=LOC103503022 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 5.3e-17
Identity = 58/69 (84.06%), Postives = 65/69 (94.20%), Query Frame = 0

Query: 1  MAVLRA-CFGIYAAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLL 60
          MAVLRA CFGIYAAV++AVFYV++L  +VS+AE SSSPAPAPTSDGTTIDQGIAYVLML+
Sbjct: 1  MAVLRASCFGIYAAVLIAVFYVLAL--SVSSAELSSSPAPAPTSDGTTIDQGIAYVLMLV 60

Query: 61 ALVLTYIIH 69
          ALVLTYIIH
Sbjct: 61 ALVLTYIIH 67

BLAST of ClCG05G008690 vs. ExPASy TrEMBL
Match: A0A6J1CHE0 (arabinogalactan peptide 22-like OS=Momordica charantia OX=3673 GN=LOC111011447 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 4.5e-16
Identity = 54/68 (79.41%), Postives = 60/68 (88.24%), Query Frame = 0

Query: 1  MAVLRACFGIYAAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLLA 60
          MAV RACFGIYA +I A+ +V+ LP  VS AEHSSSPAPAP+SDGTTIDQGIAY+LMLLA
Sbjct: 1  MAVSRACFGIYAGII-AILFVIILP--VSRAEHSSSPAPAPSSDGTTIDQGIAYILMLLA 60

Query: 61 LVLTYIIH 69
          LVLTYIIH
Sbjct: 61 LVLTYIIH 65

BLAST of ClCG05G008690 vs. TAIR 10
Match: AT5G53250.1 (arabinogalactan protein 22 )

HSP 1 Score: 70.1 bits (170), Expect = 7.8e-13
Identity = 37/54 (68.52%), Postives = 45/54 (83.33%), Query Frame = 0

Query: 15 IMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLLALVLTYIIH 69
          I+AVF ++S+ +   A  HSSSPAPAPTSDGT+IDQGIAYVLM++AL LTY IH
Sbjct: 10 ILAVFVIISVILLPIAQSHSSSPAPAPTSDGTSIDQGIAYVLMMVALALTYFIH 63

BLAST of ClCG05G008690 vs. TAIR 10
Match: AT5G24105.1 (arabinogalactan protein 41 )

HSP 1 Score: 64.3 bits (155), Expect = 4.3e-11
Identity = 39/68 (57.35%), Postives = 53/68 (77.94%), Query Frame = 0

Query: 1  MAVLRACFGIYAAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLLA 60
          M+  R  FG+  + I+++ + + LP+A +    S++PAPAPTSDGTTIDQGIAYVLML+A
Sbjct: 1  MSGSRLFFGV--STIVSIIFAILLPMAHA---QSAAPAPAPTSDGTTIDQGIAYVLMLVA 60

Query: 61 LVLTYIIH 69
          LVLTY+IH
Sbjct: 61 LVLTYLIH 63

BLAST of ClCG05G008690 vs. TAIR 10
Match: AT2G46330.1 (arabinogalactan protein 16 )

HSP 1 Score: 58.2 bits (139), Expect = 3.1e-09
Identity = 32/52 (61.54%), Postives = 40/52 (76.92%), Query Frame = 0

Query: 17 AVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLLALVLTYIIH 69
          A+F  V   +   A   S +PAPAPTSDGT+IDQGIAY+LM++ALVLTY+IH
Sbjct: 11 ALFSFVFAVILSLAGAQSLAPAPAPTSDGTSIDQGIAYLLMVVALVLTYLIH 62

BLAST of ClCG05G008690 vs. TAIR 10
Match: AT3G61640.1 (arabinogalactan protein 20 )

HSP 1 Score: 58.2 bits (139), Expect = 3.1e-09
Identity = 31/57 (54.39%), Postives = 44/57 (77.19%), Query Frame = 0

Query: 12 AAVIMAVFYVVSLPVAVSAAEHSSSPAPAPTSDGTTIDQGIAYVLMLLALVLTYIIH 69
          +  ++A+F  V   ++  A   S +PAP+PTSDGT+IDQGIAY+LM++ALVLTY+IH
Sbjct: 6  SVAVIALFAFVFAVISPFAGAQSLAPAPSPTSDGTSIDQGIAYLLMVVALVLTYLIH 62

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898060.11.4e-1989.71arabinogalactan protein 41-like [Benincasa hispida] >XP_038898069.1 arabinogalac... [more]
TYK03143.12.2e-1785.51arabinogalactan peptide 22 [Cucumis melo var. makuwa][more]
XP_022945187.13.8e-1783.82arabinogalactan peptide 22-like [Cucurbita moschata] >XP_023541894.1 arabinogala... [more]
XP_008465391.11.1e-1684.06PREDICTED: arabinogalactan peptide 22 [Cucumis melo] >KAA0050462.1 arabinogalact... [more]
XP_022140891.19.3e-1679.41arabinogalactan peptide 22-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9FK161.1e-1168.52Arabinogalactan protein 22 OS=Arabidopsis thaliana OX=3702 GN=AGP22 PE=1 SV=1[more]
Q8L9T86.0e-1057.35Arabinogalactan protein 41 OS=Arabidopsis thaliana OX=3702 GN=AGP41 PE=1 SV=1[more]
O823374.3e-0861.54Arabinogalactan protein 16 OS=Arabidopsis thaliana OX=3702 GN=AGP16 PE=1 SV=1[more]
Q9M3734.3e-0854.39Arabinogalactan protein 20 OS=Arabidopsis thaliana OX=3702 GN=AGP20 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BTZ51.1e-1785.51Arabinogalactan peptide 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A6J1G0811.8e-1783.82arabinogalactan peptide 22-like OS=Cucurbita moschata OX=3662 GN=LOC111449506 PE... [more]
A0A5A7U3P45.3e-1784.06Arabinogalactan peptide 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3CNS55.3e-1784.06arabinogalactan peptide 22 OS=Cucumis melo OX=3656 GN=LOC103503022 PE=4 SV=1[more]
A0A6J1CHE04.5e-1679.41arabinogalactan peptide 22-like OS=Momordica charantia OX=3673 GN=LOC111011447 P... [more]
Match NameE-valueIdentityDescription
AT5G53250.17.8e-1368.52arabinogalactan protein 22 [more]
AT5G24105.14.3e-1157.35arabinogalactan protein 41 [more]
AT2G46330.13.1e-0961.54arabinogalactan protein 16 [more]
AT3G61640.13.1e-0954.39arabinogalactan protein 20 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009424Arabinogalactan protein 16/20/22/41PFAMPF06376AGPcoord: 36..68
e-value: 9.5E-19
score: 67.0
IPR009424Arabinogalactan protein 16/20/22/41PANTHERPTHR33374ARABINOGALACTAN PROTEIN 20coord: 1..68
NoneNo IPR availablePANTHERPTHR33374:SF38ARABINOGALACTAN PROTEIN 41coord: 1..68

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG05G008690.1ClCG05G008690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane