ClCG04G005140 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG04G005140
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionRING-type E3 ubiquitin transferase
LocationCG_Chr04: 18788121 .. 18790691 (+)
RNA-Seq ExpressionClCG04G005140
SyntenyClCG04G005140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGAATTAGATCAAGAAGTAGTTGACCCTCCTCCCTTCTTTCTCTGTCCCATCTCTTTGCAAATCATGAAAGACCCCGTCACGATCACCTCCGGCATAACCTACGACCGAGAAAACATTGAGAAATGGTTGTTTTCCGACAAACACAAGACGTGCCCTGTCTCACAGGTTGTCTTGTCGGATTTCAACATCACTCCCAACCATACTTTAAGACGAGTTATTCAAGCTTGGTGCACAGTCAACGCTTGTAGTGGCGTCGAGAGGATGCCAACACCCAAACCTCCGGTTGACCGGAAACAAGTCGTTAGGATTCTTGCTGATGCCAAGTTATCTCCTTTCTCTCAGCAGAATTGTCTTCGTCGTCTTCGATCTATCGCTGCGGCGAATGAAACTAATAAACGTTGCATGGAATCGGCTGGAGCTGTGGAGTTCTTGGCTGGGCTTGTGTGTAGTTCTACAACCAACGTGGAGTCTGGCTTTGAAGGTACTATACAAAACTCAAACAAATTTTTGCTAATGTTTACATAATTCAAGTTTAGTTTAAAAACTAATTAAGAAAAATAGTTTTTGTTTATGAGGGCCTCTTCCATCTGGTTTTGAATTGGGTTTTTTTTTTTTAATTTTTTTTTTAATTTTTAATTTTTAATTTTATTATTATTTTTTTTATTTTTTTAATTTTTAAAAACTAAGTTTATAAATATCCGGTTTGTTTTCAAATGTAGTAAAATAAACAATATATATATATATATATATATATATATATATATATATATATATATATATTTACAAATATAACAAAAATTTATTGTCTATTGCGGTGATATTTTGCTATTATTTGTAAATATTTTTAATAGGTTTGTCATTTAAAATAATTTCTCTAAATATTATTTCCAACTATTATTTTTTTTAGATATTTTAAATTTTTTGTTTTTATCTTGTAATTTAGCAAAAATAAATACCATGGCTAGGAAATCATTATTTTCAAAAACCAAATGGTTATCATACAAGAACTAGAAAATATCTAAAAAGTTTAAAACTTAATTTTGTATCAAATAAAGTTTCTAAACTTTAAAAGGTGTCTAACAAGTCTTAAGTCTTAATGTTGTGTCTAGTAAATCTTTGACTTATTCAATATCAAAAGTTGAAATTAATATGTTAGAGTAAATTTAATCTTAAGAAAAATATTATTTTAGTCCCTAGGAGGATTTGAGTCAGGAAAACAGTTTTATCCTAAACTTTGAGGTTAATTTTTATTTATTTACTATGTTTTAGAATATTACATTTTTACTCTTGAGTTTTAAGTTTAGTTTTCATTTGATTCATACATTTCTAGATTTTACACTTTTACTCTCGAGTTTTTACTAATAATGCTCATTTTGGTCTTTAATATTAACATTCAATTAATTAATTTAAAAATAACTATGATGTGATTTGTAATTAATTTCATTCTTTTAAATTAACTAATAATTAGAATTAAAAGTGTAGATCTTAAAATTTAAATATCAAATGAAAATAAAATTAAAAACAGTAGATTTAAAAACTATAACATTTTGAAATTTAGGAAGTAAATAAAAGTTAATTTCAAAACTCAAAGGAATCAAATAGTTTGAACAATGTCCCTCAATAGTGAGTTTGAAACTATTATATTTAATTGGGATGGAAAACTGATATTGGACTGATTCGAACTTTTTGCCTCTCGTTTTAAATTTGTATAATAATTAGTAAGTTTAGAATTTAATATTGGTTATGCTTATGCAAATTGTTGATTGATTTGACTCAGATTACGCGTTTGACGACATGTCGGCAAACTTGGGGGATGAGGCTTTGAGCATTCTTCACAAACTCCAGATCTCCGAATCCGCTCTCAAATTTCTGCTCTCCAACAATGGCGGCATCTTCATCTCCACCTTAACCAAAATCTTGCAAAACCGAAGCTATTCCTCCCGAGCGTATTCAATAATGCTTCTCAATTCAATGTTCGAAGTCGCAGATCAGGTTCAAATCCTCAGTCTCACCGCCGATTTCTTCATCGAAATCGTCCAAATCCTTAAAGATCAGATCTCCAAACAAGCCTCGAAATCGGCATTGAAATTGCTAATCGGTGTCTGTTCGAGTTCCAGAAACAGAGTAAAGGCGGTTCAAGCCGGTGCGGTTCCGGTTCTGATCGATCTCCTTCTCGATCTCGATTCGTCGGAGAAGCGTTTGTGCGAGATGATTCTCGTGGTGCTTGATCTGCTGTGTGGTTGCGCCGATGGCAGGGCGGAGCTGTTGAATCACGCGGCGGGGATCGCCGTCGTGTCGAAGAAGATTTTGAGAGTTTCGTCGCTCGGAAGTGAGAAGGCGGTTGGGATCTTATGGTCCGTTGCGAGATTCTCGGCGAGCCAAACCGTCATTCAGGAAATGCTGCGGCTGGGAGTTGTGACGAAGCTTTGTTTTCTGCTTCAAGTGGTCGACGCCGGAGCGAAAGCGAAAGAGAAAGCTAAAGAGATTCTGAAGCTTCATGGTCGGCCATGGCGGAATTCTTCTTGTTTGCCTTCGAGTCTGCGATCATCTTATCCACAACATTAG

mRNA sequence

ATGGCAGAATTAGATCAAGAAGTAGTTGACCCTCCTCCCTTCTTTCTCTGTCCCATCTCTTTGCAAATCATGAAAGACCCCGTCACGATCACCTCCGGCATAACCTACGACCGAGAAAACATTGAGAAATGGTTGTTTTCCGACAAACACAAGACGTGCCCTGTCTCACAGGTTGTCTTGTCGGATTTCAACATCACTCCCAACCATACTTTAAGACGAGTTATTCAAGCTTGGTGCACAGTCAACGCTTGTAGTGGCGTCGAGAGGATGCCAACACCCAAACCTCCGGTTGACCGGAAACAAGTCGTTAGGATTCTTGCTGATGCCAAGTTATCTCCTTTCTCTCAGCAGAATTGTCTTCGTCGTCTTCGATCTATCGCTGCGGCGAATGAAACTAATAAACGTTGCATGGAATCGGCTGGAGCTGTGGAGTTCTTGGCTGGGCTTGTGTGTAGTTCTACAACCAACGTGGAGTCTGGCTTTGAAGATTACGCGTTTGACGACATGTCGGCAAACTTGGGGGATGAGGCTTTGAGCATTCTTCACAAACTCCAGATCTCCGAATCCGCTCTCAAATTTCTGCTCTCCAACAATGGCGGCATCTTCATCTCCACCTTAACCAAAATCTTGCAAAACCGAAGCTATTCCTCCCGAGCGTATTCAATAATGCTTCTCAATTCAATGTTCGAAGTCGCAGATCAGGTTCAAATCCTCAGTCTCACCGCCGATTTCTTCATCGAAATCGTCCAAATCCTTAAAGATCAGATCTCCAAACAAGCCTCGAAATCGGCATTGAAATTGCTAATCGGTGTCTGTTCGAGTTCCAGAAACAGAGTAAAGGCGGTTCAAGCCGGTGCGGTTCCGGTTCTGATCGATCTCCTTCTCGATCTCGATTCGTCGGAGAAGCGTTTGTGCGAGATGATTCTCGTGGTGCTTGATCTGCTGTGTGGTTGCGCCGATGGCAGGGCGGAGCTGTTGAATCACGCGGCGGGGATCGCCGTCGTGTCGAAGAAGATTTTGAGAGTTTCGTCGCTCGGAAGTGAGAAGGCGGTTGGGATCTTATGGTCCGTTGCGAGATTCTCGGCGAGCCAAACCGTCATTCAGGAAATGCTGCGGCTGGGAGTTGTGACGAAGCTTTGTTTTCTGCTTCAAGTGGTCGACGCCGGAGCGAAAGCGAAAGAGAAAGCTAAAGAGATTCTGAAGCTTCATGGTCGGCCATGGCGGAATTCTTCTTGTTTGCCTTCGAGTCTGCGATCATCTTATCCACAACATTAG

Coding sequence (CDS)

ATGGCAGAATTAGATCAAGAAGTAGTTGACCCTCCTCCCTTCTTTCTCTGTCCCATCTCTTTGCAAATCATGAAAGACCCCGTCACGATCACCTCCGGCATAACCTACGACCGAGAAAACATTGAGAAATGGTTGTTTTCCGACAAACACAAGACGTGCCCTGTCTCACAGGTTGTCTTGTCGGATTTCAACATCACTCCCAACCATACTTTAAGACGAGTTATTCAAGCTTGGTGCACAGTCAACGCTTGTAGTGGCGTCGAGAGGATGCCAACACCCAAACCTCCGGTTGACCGGAAACAAGTCGTTAGGATTCTTGCTGATGCCAAGTTATCTCCTTTCTCTCAGCAGAATTGTCTTCGTCGTCTTCGATCTATCGCTGCGGCGAATGAAACTAATAAACGTTGCATGGAATCGGCTGGAGCTGTGGAGTTCTTGGCTGGGCTTGTGTGTAGTTCTACAACCAACGTGGAGTCTGGCTTTGAAGATTACGCGTTTGACGACATGTCGGCAAACTTGGGGGATGAGGCTTTGAGCATTCTTCACAAACTCCAGATCTCCGAATCCGCTCTCAAATTTCTGCTCTCCAACAATGGCGGCATCTTCATCTCCACCTTAACCAAAATCTTGCAAAACCGAAGCTATTCCTCCCGAGCGTATTCAATAATGCTTCTCAATTCAATGTTCGAAGTCGCAGATCAGGTTCAAATCCTCAGTCTCACCGCCGATTTCTTCATCGAAATCGTCCAAATCCTTAAAGATCAGATCTCCAAACAAGCCTCGAAATCGGCATTGAAATTGCTAATCGGTGTCTGTTCGAGTTCCAGAAACAGAGTAAAGGCGGTTCAAGCCGGTGCGGTTCCGGTTCTGATCGATCTCCTTCTCGATCTCGATTCGTCGGAGAAGCGTTTGTGCGAGATGATTCTCGTGGTGCTTGATCTGCTGTGTGGTTGCGCCGATGGCAGGGCGGAGCTGTTGAATCACGCGGCGGGGATCGCCGTCGTGTCGAAGAAGATTTTGAGAGTTTCGTCGCTCGGAAGTGAGAAGGCGGTTGGGATCTTATGGTCCGTTGCGAGATTCTCGGCGAGCCAAACCGTCATTCAGGAAATGCTGCGGCTGGGAGTTGTGACGAAGCTTTGTTTTCTGCTTCAAGTGGTCGACGCCGGAGCGAAAGCGAAAGAGAAAGCTAAAGAGATTCTGAAGCTTCATGGTCGGCCATGGCGGAATTCTTCTTGTTTGCCTTCGAGTCTGCGATCATCTTATCCACAACATTAG

Protein sequence

MAELDQEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVLSDFNITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQNCLRRLRSIAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFDDMSANLGDEALSILHKLQISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQILSLTADFFIEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLDSSEKRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSVARFSASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSSLRSSYPQH
Homology
BLAST of ClCG04G005140 vs. NCBI nr
Match: XP_038882772.1 (E3 ubiquitin-protein ligase PUB22-like [Benincasa hispida])

HSP 1 Score: 758.8 bits (1958), Expect = 2.4e-215
Identity = 395/424 (93.16%), Postives = 410/424 (96.70%), Query Frame = 0

Query: 1   MAELDQEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVL 60
           MAELDQEV+DPPPFFLCPISLQIMKDPVTI SGITYDRENIEKWLFSDKHKTCPVS+VVL
Sbjct: 1   MAELDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVL 60

Query: 61  SDFNITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120
            DF+ITPNHTLRRVIQAWCT NA SGVER+PTPKPPVDRKQVVRILADAKLSPFSQQNCL
Sbjct: 61  LDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120

Query: 121 RRLRSIAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFDDMSANLGDEALSI 180
           RRLRSIAAANETNKRCME+AG VEFLAGLVCSSTTNVESG EDYAFDDMSANLGDEALSI
Sbjct: 121 RRLRSIAAANETNKRCMEAAGVVEFLAGLVCSSTTNVESGLEDYAFDDMSANLGDEALSI 180

Query: 181 LHKLQISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQILSL 240
           L+KLQISES+LKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQ+QIL+L
Sbjct: 181 LYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQILNL 240

Query: 241 TADFFIEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLDSS 300
           T DFFIEIVQILKDQISKQASKSALKLLIGVCSS RNRVKAV+AGAVPVL+DLLLDLDSS
Sbjct: 241 TTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLDSS 300

Query: 301 EKRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSVARF 360
           EKRL EMILVVLDLLCGCADGRA LLNHAAGIAVVSKKILRVSSLGSEKAVGIL SVARF
Sbjct: 301 EKRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVARF 360

Query: 361 SASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSSLRSS 420
           SASQ+V+QEM+RLGVVTKLCFLLQVV AG KAKEKAKEILKLHGR WRNSSCLPSSLRS+
Sbjct: 361 SASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLRSA 420

Query: 421 YPQH 425
           YPQH
Sbjct: 421 YPQH 424

BLAST of ClCG04G005140 vs. NCBI nr
Match: XP_008441961.1 (PREDICTED: E3 ubiquitin-protein ligase PUB22 [Cucumis melo] >KAA0060143.1 E3 ubiquitin-protein ligase PUB22 [Cucumis melo var. makuwa] >TYK08456.1 E3 ubiquitin-protein ligase PUB22 [Cucumis melo var. makuwa])

HSP 1 Score: 719.9 bits (1857), Expect = 1.3e-203
Identity = 372/426 (87.32%), Postives = 402/426 (94.37%), Query Frame = 0

Query: 1   MAELD--QEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQV 60
           MAELD   +VVDPPP+FLCPISLQIMKDPVTI SGITYDRE+IEKWLFSDKHKTCPVS +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSDFNITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQN 120
           VLSDF++TPNHTLRRVIQAWCTVNA  GVER+PTPKPPVDR+QVVRILADAKLSPFSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLRSIAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFDDMSANLGDEAL 180
           CL RLRSIAAANE+NKRCMESAG VEFLAG+VC+STTN+E G EDYAFDDMSAN  DEAL
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL 180

Query: 181 SILHKLQISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQIL 240
            ILHKLQISES+LKFLLSNNGGIFISTLTKILQNRSYSSR+YS+MLLNSMFEVADQ+QI 
Sbjct: 181 IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240

Query: 241 SLTADFFIEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLD 300
           +LTADFFIEI+QILKDQISKQASKSALKLLI V SS RNRVKAVQAGAVPVLIDLLLDLD
Sbjct: 241 NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300

Query: 301 SSE-KRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSV 360
           SSE KRLCEMILV+LD LCGCADGRAELLNHAAGIAVVSKKILR+S++GSEKAVGILWS+
Sbjct: 301 SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI 360

Query: 361 ARFSASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSSL 420
           ARFS SQ+V+QEM+R+GVVTKLCFLLQVV AG KAKEKAKEILKLHGR WRNSSCLPS+L
Sbjct: 361 ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420

Query: 421 RSSYPQ 424
           RS+YPQ
Sbjct: 421 RSAYPQ 426

BLAST of ClCG04G005140 vs. NCBI nr
Match: XP_023543358.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 705.7 bits (1820), Expect = 2.5e-199
Identity = 366/424 (86.32%), Postives = 398/424 (93.87%), Query Frame = 0

Query: 1   MAELDQEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVL 60
           MAELDQE +DPPPFFLCPISLQIMKDPVT+ SGITYDRE+IEKWLFSD+HKTCPV+QVVL
Sbjct: 1   MAELDQE-LDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVL 60

Query: 61  SDFNITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120
           S F+ITPNHTLRRVIQAWCTVNAC G+ER+PTPKPPVD KQVVRILADAKLSPFSQQNCL
Sbjct: 61  SSFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCL 120

Query: 121 RRLRSIAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAF--DDMSANLGDEAL 180
           RRLRSIAA +E+NKRCME+AGAVEFLAG+VC+S TNVE   EDYA+  DD  ANLGDEAL
Sbjct: 121 RRLRSIAAGSESNKRCMEAAGAVEFLAGIVCNS-TNVEFSSEDYAYLIDDQFANLGDEAL 180

Query: 181 SILHKLQISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQIL 240
           SIL+KLQISES LKFLL+NNGGIF+STLTKILQN +Y SRAYS+MLL SMFEVADQ+QIL
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 241 SLTADFFIEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLD 300
           SL  DFF+EIVQILKDQISKQASKSALKLLIG+CSSSRNRVKAV+AGAVPV+IDLLLDLD
Sbjct: 241 SLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLD 300

Query: 301 SSEKRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSVA 360
           SSEKR CEMILV LDLLCGCADGRAE+LNHAAGIAVVSKKILRVS+LGSEKAVGIL S+A
Sbjct: 301 SSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIA 360

Query: 361 RFSASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSSLR 420
           RFSAS++V+QEM+RLGVVTKLCFLLQVV AG KAKEKAKEILKLHGR WRNSSCLPSSLR
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLR 420

Query: 421 SSYP 423
           SS+P
Sbjct: 421 SSFP 422

BLAST of ClCG04G005140 vs. NCBI nr
Match: XP_022978192.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 704.5 bits (1817), Expect = 5.5e-199
Identity = 364/424 (85.85%), Postives = 398/424 (93.87%), Query Frame = 0

Query: 1   MAELDQEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVL 60
           MAELDQE +DPPPFFLCPISLQIMKDPVT+ SGITYDRE+IE+WLFSD+HKTCPV+QVVL
Sbjct: 1   MAELDQE-LDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVL 60

Query: 61  SDFNITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120
           S F+ITPNHT+RRVIQAWCT+NAC G+ER+PTPKPP+D KQVVRILADAKLSPFSQQNCL
Sbjct: 61  SSFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCL 120

Query: 121 RRLRSIAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAF--DDMSANLGDEAL 180
           RRLRSIAAA+E+NKRCME+AGAVEFLAG+VC+S TNVE   EDYA+  DD  ANLGDEAL
Sbjct: 121 RRLRSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQFANLGDEAL 180

Query: 181 SILHKLQISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQIL 240
           SIL+KLQISES LKFLL+NNGGIF+STLTKILQN +Y SRAYS+MLL SMFEVADQ+QIL
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 241 SLTADFFIEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLD 300
           SL  DFFIEIVQILKDQISKQASKSALKLLIG+CSSSRNRVKAVQAGA+PVLIDLLLDLD
Sbjct: 241 SLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLD 300

Query: 301 SSEKRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSVA 360
           SSEKR CEMILV LDLLC CADGRAE+LNHAAGIAVVSKKILRVS+LGSEKAVGIL S+A
Sbjct: 301 SSEKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIA 360

Query: 361 RFSASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSSLR 420
           RFSAS++V+QEM+RLGVVTKLCFLLQVV AG KAKEKAKEILKLHGR WRNSSCLPSSLR
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLR 420

Query: 421 SSYP 423
           SS+P
Sbjct: 421 SSFP 422

BLAST of ClCG04G005140 vs. NCBI nr
Match: KAG7033684.1 (E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 701.8 bits (1810), Expect = 3.6e-198
Identity = 365/424 (86.08%), Postives = 396/424 (93.40%), Query Frame = 0

Query: 1   MAELDQEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVL 60
           MAELDQE +DPPPFFLCPISLQIMKDPVT+ SGITYDR++IEKWLFSD HKTCPV+QVVL
Sbjct: 1   MAELDQE-LDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVL 60

Query: 61  SDFNITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120
           S F+ITPN TLRRVIQAWCTVNAC G+ER+PTPKPPVD KQVVRILADAKLSPFSQQNCL
Sbjct: 61  SSFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCL 120

Query: 121 RRLRSIAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAF--DDMSANLGDEAL 180
           RRLRSIAAA+E+NKRCME+AGAVEFLAG+VC+S TNVE   EDYA+  DD  ANLGDEAL
Sbjct: 121 RRLRSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQLANLGDEAL 180

Query: 181 SILHKLQISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQIL 240
           SILHKLQISES LKFLL+NNGGIF+STLTKILQN +Y SRAYS+MLL SMFEVADQ+QIL
Sbjct: 181 SILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 241 SLTADFFIEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLD 300
           SL  DFF+EIVQILKDQISKQASKSALKLLIG+CSSSRNRVKAV+AGAVPVLIDLLLDLD
Sbjct: 241 SLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLD 300

Query: 301 SSEKRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSVA 360
           SSEKR CEMILV LDLLCGCADGRAE+LNHAAGIA+VSKKILRVS+LGSEKAVGIL S+A
Sbjct: 301 SSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIA 360

Query: 361 RFSASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSSLR 420
           RFSAS++V+QEM+RLGVVTKLCFLLQV  AG KAKEKAKEILKLHGR WRNSSCLPSSLR
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLR 420

Query: 421 SSYP 423
           SS+P
Sbjct: 421 SSFP 422

BLAST of ClCG04G005140 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 3.8e-118
Identity = 228/436 (52.29%), Postives = 309/436 (70.87%), Query Frame = 0

Query: 4   LDQEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVLSDF 63
           +DQE ++ P FFLCPISL IMKDPV +++GITYDRE+IEKWLFS K  +CPV++ V+++ 
Sbjct: 1   MDQE-IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITET 60

Query: 64  NITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQNCLRRL 123
           ++TPNHTLRR+IQ+WCT+NA  G+ER+PTPKPP+ + ++ +++ ++  S  +Q  CL+RL
Sbjct: 61  DLTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRL 120

Query: 124 RSIAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFD---------------D 183
           R I + N TNKRC+E+A   EFLA +V +S     S     +                 D
Sbjct: 121 RQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFD 180

Query: 184 MSANLGDEALSILHKLQISESALKFLLSNNGGI-FISTLTKILQNRSYSSRAYSIMLLNS 243
            S +L DEALS+L+ L  SE+ALK LL+N  G   + TLTKI+Q   Y SRAY+ +LL  
Sbjct: 181 SSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKK 240

Query: 244 MFEVADQVQILSLTADFFIEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAV 303
           + EVAD +QI+ L  + F E++QIL DQIS +A++SA+++L+  C   RNR KAV+ G +
Sbjct: 241 LLEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTI 300

Query: 304 PVLIDLLL-DLDSSEKRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLG 363
            ++I+LL+ D  SSE+R  EM +VVLD+LC CA+GRAE LNH A IAVVSKKILRVS + 
Sbjct: 301 SMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQIT 360

Query: 364 SEKAVGILWSVARFSASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRP 423
           SE+AV +L SV RF A+ +++QEML+LGVV KLC +LQ V  G K KEKAKE+LKLH R 
Sbjct: 361 SERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARV 420

BLAST of ClCG04G005140 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 6.7e-115
Identity = 215/418 (51.44%), Postives = 303/418 (72.49%), Query Frame = 0

Query: 7   EVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVLSDFNIT 66
           E ++ PPFFLCPISL+IMKDPV +++GITYDR++IEKWLF+ K  +CPV++  ++D ++T
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLT 67

Query: 67  PNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQNCLRRLRSI 126
           PNHTLRR+IQ+WCT+NA  GVER+PTP+PP+ + ++ +++ D+  S  +Q  CL+RLR I
Sbjct: 68  PNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI 127

Query: 127 AAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFDDMSANLGDEALSILHKLQI 186
            + N TNKRC+E+AG  EFLA +V + + N               +L DEAL++L+ L+ 
Sbjct: 128 VSENATNKRCLEAAGVPEFLANIVSNDSEN--------------GSLTDEALNLLYHLET 187

Query: 187 SESALKFLLSN-NGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQILSLTADFF 246
           SE+ LK LL+N      + +LTKI+Q   Y SR Y+ +LL ++ EVAD +Q ++L  + F
Sbjct: 188 SETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVF 247

Query: 247 IEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLD-SSEKRL 306
            E+VQIL D+IS++A+K+A+ +L+ +C   RNR KAV+AG + V+I+LL+D   +SE+R 
Sbjct: 248 TEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRG 307

Query: 307 CEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSVARFSASQ 366
            EM +VVLDLLC CA+GRAE LNH A IAVV KKILRVS   S++AV +L SV RF A+ 
Sbjct: 308 PEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATP 367

Query: 367 TVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSSLRSSYP 423
            ++ EML+LGVV KLC +LQ V  G K KEKAKE+LKLH R W++S CLP ++  +YP
Sbjct: 368 ALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of ClCG04G005140 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 4.5e-63
Identity = 166/452 (36.73%), Postives = 244/452 (53.98%), Query Frame = 0

Query: 5   DQEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVLS-DF 64
           ++E ++ P +F+CPISL+IMKDPVT  SGITYDR+NI KWL  +K  +CPV++  L  D 
Sbjct: 4   EEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDS 63

Query: 65  NITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAK---LSPFSQQNCL 124
           ++TPNH LRR+IQ WC  N   GV R+ TP+ P  +  VV  + + K        ++  L
Sbjct: 64  DLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETL 123

Query: 125 RRLRSIAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFDDMSANLGDEALSI 184
           ++L  + A +  N+R M   G  + L   V   T+  E G              DE+L +
Sbjct: 124 QKL-EVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDG-------RRRIKGLDESLRL 183

Query: 185 LHKLQISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQILSL 244
           LH + I  +  K +L  N  + + +LT +L    + S+AY+I+LL ++ E      +  L
Sbjct: 184 LHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERL 243

Query: 245 TADFFIEIVQILKDQIS-----------------------------------KQASKSAL 304
             + F  I+  LKD ++                                   KQA  +AL
Sbjct: 244 NPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAAL 303

Query: 305 KLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLDSSEKRLCEMILVVLDLLCGCADGRAEL 364
            +L+   S SRNR   V  GAV  LI+L +   + EKR+ E++L VL  LC CA+GRAE+
Sbjct: 304 MILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANGRAEI 363

Query: 365 LNHAAGIAVVSKKILRVSSLGSEKAVGILWSVARFSASQTVIQEMLRLGVVTKLCFLLQV 418
           L H  GIAVV+K++LRVS    ++A+ IL +V++FS    V++EM+ +G V KLC +L  
Sbjct: 364 LAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLG- 423

BLAST of ClCG04G005140 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.1e-48
Identity = 145/416 (34.86%), Postives = 225/416 (54.09%), Query Frame = 0

Query: 12  PPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVLSDFNITPNHTL 71
           P  F CPISL++M+DPVT+ +G TYDR +IE W+    + TCPV++  LSDF + PNHTL
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPNHTL 74

Query: 72  RRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAK------LSPFSQQNCLRRLRS 131
           RR+IQ WC  N  +GVER+PTPK P D   V  +L+ A       +S  S+   LRRLR 
Sbjct: 75  RRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRRLRG 134

Query: 132 IAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFDDMSANLGDEALSILHKLQ 191
            A  ++ N+  + +  A E L  ++ S TT              S+ L  E+L++L  L 
Sbjct: 135 FARDSDKNRVLIAAHNATEILIKILFSETT--------------SSELVSESLALLVMLP 194

Query: 192 ISESALKFLLSNNGGIFISTLTKILQNRSYSSR---AYSIMLLNSMFEVADQVQILSLTA 251
           I+E      +S++ G  +  LT++L + S  +R   A  I ++++  + AD    +S + 
Sbjct: 195 ITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSE 254

Query: 252 DFFIEIVQILKDQI-SKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLID-LLLDLDSS 311
             F  ++ +L++ I S++A K  +K L  +CS    R  A+ AGA  +LID L  D D  
Sbjct: 255 SVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRC 314

Query: 312 EKRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSVARF 371
           +    E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  
Sbjct: 315 D---TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGAL--LALC 374

Query: 372 SASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSS 417
           +A +   +E    GVV +L  ++Q  +   +AK+KA+++LKL    W + +   +S
Sbjct: 375 TAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYNSFANS 409

BLAST of ClCG04G005140 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.6e-47
Identity = 144/415 (34.70%), Postives = 221/415 (53.25%), Query Frame = 0

Query: 12  PPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVLSDFNITPNHTL 71
           P  F CPISL +M DPVTI++G TYDR +I+ W+ +  + TCPV++V LSDF + PNHTL
Sbjct: 15  PYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFTLIPNHTL 74

Query: 72  RRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAK------LSPFSQQNCLRRLRS 131
           RR+IQ WC  N  +GVER+PTPK P D   V  +L+ A       +S  S+   +RRLR 
Sbjct: 75  RRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRLRG 134

Query: 132 IAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFDDMSANLGDEALSILHKLQ 191
           +A  +E N+  +    A E L  ++          F D     +S+ L  E+L++L  L 
Sbjct: 135 LARDSEKNRVLIAGHNAREILVRIL----------FADIETTSLSSELVSESLALLVLLH 194

Query: 192 ISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVA---DQVQILSLTA 251
           ++E+  + + S+     +  +T++L + S   R  +  L+  +   A   D   I+S + 
Sbjct: 195 MTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSD 254

Query: 252 DFFIEIVQILKDQI-SKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLID-LLLDLDSS 311
             F  ++ +LK+ I S++A K  +K +  +C   + R  A+ AGA  +LID L  D D  
Sbjct: 255 SIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRC 314

Query: 312 EKRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSVARF 371
           +    E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  
Sbjct: 315 D---TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL--LALC 374

Query: 372 SASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPS 416
           +A +    E    G+VT+L  L+Q  D   +AK KA+ +LKL    W + S + S
Sbjct: 375 TAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDSTVHS 410

BLAST of ClCG04G005140 vs. ExPASy TrEMBL
Match: A0A5A7UVZ1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00730 PE=4 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 6.1e-204
Identity = 372/426 (87.32%), Postives = 402/426 (94.37%), Query Frame = 0

Query: 1   MAELD--QEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQV 60
           MAELD   +VVDPPP+FLCPISLQIMKDPVTI SGITYDRE+IEKWLFSDKHKTCPVS +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSDFNITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQN 120
           VLSDF++TPNHTLRRVIQAWCTVNA  GVER+PTPKPPVDR+QVVRILADAKLSPFSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLRSIAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFDDMSANLGDEAL 180
           CL RLRSIAAANE+NKRCMESAG VEFLAG+VC+STTN+E G EDYAFDDMSAN  DEAL
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL 180

Query: 181 SILHKLQISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQIL 240
            ILHKLQISES+LKFLLSNNGGIFISTLTKILQNRSYSSR+YS+MLLNSMFEVADQ+QI 
Sbjct: 181 IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240

Query: 241 SLTADFFIEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLD 300
           +LTADFFIEI+QILKDQISKQASKSALKLLI V SS RNRVKAVQAGAVPVLIDLLLDLD
Sbjct: 241 NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300

Query: 301 SSE-KRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSV 360
           SSE KRLCEMILV+LD LCGCADGRAELLNHAAGIAVVSKKILR+S++GSEKAVGILWS+
Sbjct: 301 SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI 360

Query: 361 ARFSASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSSL 420
           ARFS SQ+V+QEM+R+GVVTKLCFLLQVV AG KAKEKAKEILKLHGR WRNSSCLPS+L
Sbjct: 361 ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420

Query: 421 RSSYPQ 424
           RS+YPQ
Sbjct: 421 RSAYPQ 426

BLAST of ClCG04G005140 vs. ExPASy TrEMBL
Match: A0A1S3B4K2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 6.1e-204
Identity = 372/426 (87.32%), Postives = 402/426 (94.37%), Query Frame = 0

Query: 1   MAELD--QEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQV 60
           MAELD   +VVDPPP+FLCPISLQIMKDPVTI SGITYDRE+IEKWLFSDKHKTCPVS +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSDFNITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQN 120
           VLSDF++TPNHTLRRVIQAWCTVNA  GVER+PTPKPPVDR+QVVRILADAKLSPFSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLRSIAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFDDMSANLGDEAL 180
           CL RLRSIAAANE+NKRCMESAG VEFLAG+VC+STTN+E G EDYAFDDMSAN  DEAL
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL 180

Query: 181 SILHKLQISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQIL 240
            ILHKLQISES+LKFLLSNNGGIFISTLTKILQNRSYSSR+YS+MLLNSMFEVADQ+QI 
Sbjct: 181 IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240

Query: 241 SLTADFFIEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLD 300
           +LTADFFIEI+QILKDQISKQASKSALKLLI V SS RNRVKAVQAGAVPVLIDLLLDLD
Sbjct: 241 NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300

Query: 301 SSE-KRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSV 360
           SSE KRLCEMILV+LD LCGCADGRAELLNHAAGIAVVSKKILR+S++GSEKAVGILWS+
Sbjct: 301 SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI 360

Query: 361 ARFSASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSSL 420
           ARFS SQ+V+QEM+R+GVVTKLCFLLQVV AG KAKEKAKEILKLHGR WRNSSCLPS+L
Sbjct: 361 ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420

Query: 421 RSSYPQ 424
           RS+YPQ
Sbjct: 421 RSAYPQ 426

BLAST of ClCG04G005140 vs. ExPASy TrEMBL
Match: A0A6J1IKG7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 PE=4 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 2.7e-199
Identity = 364/424 (85.85%), Postives = 398/424 (93.87%), Query Frame = 0

Query: 1   MAELDQEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVL 60
           MAELDQE +DPPPFFLCPISLQIMKDPVT+ SGITYDRE+IE+WLFSD+HKTCPV+QVVL
Sbjct: 1   MAELDQE-LDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVL 60

Query: 61  SDFNITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120
           S F+ITPNHT+RRVIQAWCT+NAC G+ER+PTPKPP+D KQVVRILADAKLSPFSQQNCL
Sbjct: 61  SSFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCL 120

Query: 121 RRLRSIAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAF--DDMSANLGDEAL 180
           RRLRSIAAA+E+NKRCME+AGAVEFLAG+VC+S TNVE   EDYA+  DD  ANLGDEAL
Sbjct: 121 RRLRSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQFANLGDEAL 180

Query: 181 SILHKLQISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQIL 240
           SIL+KLQISES LKFLL+NNGGIF+STLTKILQN +Y SRAYS+MLL SMFEVADQ+QIL
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 241 SLTADFFIEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLD 300
           SL  DFFIEIVQILKDQISKQASKSALKLLIG+CSSSRNRVKAVQAGA+PVLIDLLLDLD
Sbjct: 241 SLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLD 300

Query: 301 SSEKRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSVA 360
           SSEKR CEMILV LDLLC CADGRAE+LNHAAGIAVVSKKILRVS+LGSEKAVGIL S+A
Sbjct: 301 SSEKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIA 360

Query: 361 RFSASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSSLR 420
           RFSAS++V+QEM+RLGVVTKLCFLLQVV AG KAKEKAKEILKLHGR WRNSSCLPSSLR
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLR 420

Query: 421 SSYP 423
           SS+P
Sbjct: 421 SSFP 422

BLAST of ClCG04G005140 vs. ExPASy TrEMBL
Match: A0A6J1GGQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790 PE=4 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 7.2e-197
Identity = 361/424 (85.14%), Postives = 396/424 (93.40%), Query Frame = 0

Query: 1   MAELDQEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVL 60
           MAELDQE +DPPPFFLCPISLQIMKDPVT+ SGITYDRE+IE+WLFSD+HKTCP++QVVL
Sbjct: 1   MAELDQE-LDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVL 60

Query: 61  SDFNITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120
           S+F+ITPNHTLRRVIQAWCTVNAC GVER+PTPKPPVD KQVVRILADAKLS FSQQNCL
Sbjct: 61  SNFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCL 120

Query: 121 RRLRSIAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAF--DDMSANLGDEAL 180
           RRL+SIAAA+E+NKRCME+AGAVEFLAG+VC+S TNVE   EDYA+  DD  ANLG EAL
Sbjct: 121 RRLQSIAAASESNKRCMEAAGAVEFLAGIVCNS-TNVEFSLEDYAYLIDDQLANLGFEAL 180

Query: 181 SILHKLQISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQIL 240
           SIL+KLQISES LKFLL+NNGGIF+STLTKILQN +Y SR YS+MLL SMFEVADQ+QIL
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQIL 240

Query: 241 SLTADFFIEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLD 300
           SL  DFF+EIVQILKDQISKQASKSALKLLIG+CSSSRNRVKAV+AGAVPVLIDLLLDLD
Sbjct: 241 SLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLD 300

Query: 301 SSEKRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSVA 360
           SSEKR CEMILV LDLLCGCADGRAE+LNHAAGIA+VSKKILRVS+LGSEKAVGIL S+A
Sbjct: 301 SSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIA 360

Query: 361 RFSASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSSLR 420
           RFSAS++V+QEM+RLGVVTKLCFLLQV  AG KAKEKAKEILKLHGR WRNSSCLPSSLR
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLR 420

Query: 421 SSYP 423
           SS+P
Sbjct: 421 SSFP 422

BLAST of ClCG04G005140 vs. ExPASy TrEMBL
Match: A0A0A0L1F5 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G194780 PE=4 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 1.2e-194
Identity = 359/425 (84.47%), Postives = 392/425 (92.24%), Query Frame = 0

Query: 1   MAELDQEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVL 60
           MAELDQ V+DPPP+FLCPIS QIMKDPVT+ SGITYDRE+IEKWL S KH TCPVS +VL
Sbjct: 1   MAELDQ-VLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVL 60

Query: 61  SDFNITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120
           S F+ITPNHTLRRVIQAWCT+NA  GVER+PTPKPPVDR+QVVRILA A LSPFSQ+N L
Sbjct: 61  SHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSL 120

Query: 121 RRLRSIAAANETNKRCMESAGAVEFLAGLVC-SSTTNVESGFEDYAFDDMSANLGDEALS 180
            RLRSIA ANE+NKRCMESAG VEFLAG+VC +STTN+E G EDYAFDDMSAN  DEAL 
Sbjct: 121 CRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALL 180

Query: 181 ILHKLQISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQILS 240
           ILHKLQ+SES+LKFLLSNNGGIF+STLTKILQNRSYSSR+YS+MLLNSMF+VADQ+QI +
Sbjct: 181 ILHKLQVSESSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFQVADQIQIQN 240

Query: 241 LTADFFIEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLDS 300
           LTADFF EI+QILKDQISKQASKSALKLLI V SS RNRVKAVQAGAVPVLIDLLLDLDS
Sbjct: 241 LTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDS 300

Query: 301 SE-KRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSVA 360
           SE KRLCEMILV+LDLLC CADGRAELLNHAAGIAVVSKKILRVSS+GSEKAVGILWSVA
Sbjct: 301 SENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVA 360

Query: 361 RFSASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSSLR 420
           +FS SQ+V+QEM+R+GVVTKLCF+LQVV AG KAKEKAKEILKLHGR WRNSSCLPS+LR
Sbjct: 361 KFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLR 420

Query: 421 SSYPQ 424
           S+YPQ
Sbjct: 421 SAYPQ 424

BLAST of ClCG04G005140 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 426.4 bits (1095), Expect = 2.7e-119
Identity = 228/436 (52.29%), Postives = 309/436 (70.87%), Query Frame = 0

Query: 4   LDQEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVLSDF 63
           +DQE ++ P FFLCPISL IMKDPV +++GITYDRE+IEKWLFS K  +CPV++ V+++ 
Sbjct: 1   MDQE-IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITET 60

Query: 64  NITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQNCLRRL 123
           ++TPNHTLRR+IQ+WCT+NA  G+ER+PTPKPP+ + ++ +++ ++  S  +Q  CL+RL
Sbjct: 61  DLTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRL 120

Query: 124 RSIAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFD---------------D 183
           R I + N TNKRC+E+A   EFLA +V +S     S     +                 D
Sbjct: 121 RQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFD 180

Query: 184 MSANLGDEALSILHKLQISESALKFLLSNNGGI-FISTLTKILQNRSYSSRAYSIMLLNS 243
            S +L DEALS+L+ L  SE+ALK LL+N  G   + TLTKI+Q   Y SRAY+ +LL  
Sbjct: 181 SSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKK 240

Query: 244 MFEVADQVQILSLTADFFIEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAV 303
           + EVAD +QI+ L  + F E++QIL DQIS +A++SA+++L+  C   RNR KAV+ G +
Sbjct: 241 LLEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTI 300

Query: 304 PVLIDLLL-DLDSSEKRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLG 363
            ++I+LL+ D  SSE+R  EM +VVLD+LC CA+GRAE LNH A IAVVSKKILRVS + 
Sbjct: 301 SMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQIT 360

Query: 364 SEKAVGILWSVARFSASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRP 423
           SE+AV +L SV RF A+ +++QEML+LGVV KLC +LQ V  G K KEKAKE+LKLH R 
Sbjct: 361 SERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARV 420

BLAST of ClCG04G005140 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 415.6 bits (1067), Expect = 4.7e-116
Identity = 215/418 (51.44%), Postives = 303/418 (72.49%), Query Frame = 0

Query: 7   EVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVLSDFNIT 66
           E ++ PPFFLCPISL+IMKDPV +++GITYDR++IEKWLF+ K  +CPV++  ++D ++T
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLT 67

Query: 67  PNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAKLSPFSQQNCLRRLRSI 126
           PNHTLRR+IQ+WCT+NA  GVER+PTP+PP+ + ++ +++ D+  S  +Q  CL+RLR I
Sbjct: 68  PNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI 127

Query: 127 AAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFDDMSANLGDEALSILHKLQI 186
            + N TNKRC+E+AG  EFLA +V + + N               +L DEAL++L+ L+ 
Sbjct: 128 VSENATNKRCLEAAGVPEFLANIVSNDSEN--------------GSLTDEALNLLYHLET 187

Query: 187 SESALKFLLSN-NGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQILSLTADFF 246
           SE+ LK LL+N      + +LTKI+Q   Y SR Y+ +LL ++ EVAD +Q ++L  + F
Sbjct: 188 SETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVF 247

Query: 247 IEIVQILKDQISKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLD-SSEKRL 306
            E+VQIL D+IS++A+K+A+ +L+ +C   RNR KAV+AG + V+I+LL+D   +SE+R 
Sbjct: 248 TEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRG 307

Query: 307 CEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSVARFSASQ 366
            EM +VVLDLLC CA+GRAE LNH A IAVV KKILRVS   S++AV +L SV RF A+ 
Sbjct: 308 PEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATP 367

Query: 367 TVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSSLRSSYP 423
            ++ EML+LGVV KLC +LQ V  G K KEKAKE+LKLH R W++S CLP ++  +YP
Sbjct: 368 ALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of ClCG04G005140 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 243.4 bits (620), Expect = 3.2e-64
Identity = 166/452 (36.73%), Postives = 244/452 (53.98%), Query Frame = 0

Query: 5   DQEVVDPPPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVLS-DF 64
           ++E ++ P +F+CPISL+IMKDPVT  SGITYDR+NI KWL  +K  +CPV++  L  D 
Sbjct: 18  EEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDS 77

Query: 65  NITPNHTLRRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAK---LSPFSQQNCL 124
           ++TPNH LRR+IQ WC  N   GV R+ TP+ P  +  VV  + + K        ++  L
Sbjct: 78  DLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETL 137

Query: 125 RRLRSIAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFDDMSANLGDEALSI 184
           ++L  + A +  N+R M   G  + L   V   T+  E G              DE+L +
Sbjct: 138 QKL-EVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDG-------RRRIKGLDESLRL 197

Query: 185 LHKLQISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQVQILSL 244
           LH + I  +  K +L  N  + + +LT +L    + S+AY+I+LL ++ E      +  L
Sbjct: 198 LHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERL 257

Query: 245 TADFFIEIVQILKDQIS-----------------------------------KQASKSAL 304
             + F  I+  LKD ++                                   KQA  +AL
Sbjct: 258 NPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAAL 317

Query: 305 KLLIGVCSSSRNRVKAVQAGAVPVLIDLLLDLDSSEKRLCEMILVVLDLLCGCADGRAEL 364
            +L+   S SRNR   V  GAV  LI+L +   + EKR+ E++L VL  LC CA+GRAE+
Sbjct: 318 MILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANGRAEI 377

Query: 365 LNHAAGIAVVSKKILRVSSLGSEKAVGILWSVARFSASQTVIQEMLRLGVVTKLCFLLQV 418
           L H  GIAVV+K++LRVS    ++A+ IL +V++FS    V++EM+ +G V KLC +L  
Sbjct: 378 LAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLG- 437

BLAST of ClCG04G005140 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 195.7 bits (496), Expect = 7.7e-50
Identity = 145/416 (34.86%), Postives = 225/416 (54.09%), Query Frame = 0

Query: 12  PPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVLSDFNITPNHTL 71
           P  F CPISL++M+DPVT+ +G TYDR +IE W+    + TCPV++  LSDF + PNHTL
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPNHTL 74

Query: 72  RRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAK------LSPFSQQNCLRRLRS 131
           RR+IQ WC  N  +GVER+PTPK P D   V  +L+ A       +S  S+   LRRLR 
Sbjct: 75  RRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRRLRG 134

Query: 132 IAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFDDMSANLGDEALSILHKLQ 191
            A  ++ N+  + +  A E L  ++ S TT              S+ L  E+L++L  L 
Sbjct: 135 FARDSDKNRVLIAAHNATEILIKILFSETT--------------SSELVSESLALLVMLP 194

Query: 192 ISESALKFLLSNNGGIFISTLTKILQNRSYSSR---AYSIMLLNSMFEVADQVQILSLTA 251
           I+E      +S++ G  +  LT++L + S  +R   A  I ++++  + AD    +S + 
Sbjct: 195 ITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSE 254

Query: 252 DFFIEIVQILKDQI-SKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLID-LLLDLDSS 311
             F  ++ +L++ I S++A K  +K L  +CS    R  A+ AGA  +LID L  D D  
Sbjct: 255 SVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRC 314

Query: 312 EKRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSVARF 371
           +    E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  
Sbjct: 315 D---TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGAL--LALC 374

Query: 372 SASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPSS 417
           +A +   +E    GVV +L  ++Q  +   +AK+KA+++LKL    W + +   +S
Sbjct: 375 TAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYNSFANS 409

BLAST of ClCG04G005140 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 191.8 bits (486), Expect = 1.1e-48
Identity = 144/415 (34.70%), Postives = 221/415 (53.25%), Query Frame = 0

Query: 12  PPFFLCPISLQIMKDPVTITSGITYDRENIEKWLFSDKHKTCPVSQVVLSDFNITPNHTL 71
           P  F CPISL +M DPVTI++G TYDR +I+ W+ +  + TCPV++V LSDF + PNHTL
Sbjct: 15  PYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFTLIPNHTL 74

Query: 72  RRVIQAWCTVNACSGVERMPTPKPPVDRKQVVRILADAK------LSPFSQQNCLRRLRS 131
           RR+IQ WC  N  +GVER+PTPK P D   V  +L+ A       +S  S+   +RRLR 
Sbjct: 75  RRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRLRG 134

Query: 132 IAAANETNKRCMESAGAVEFLAGLVCSSTTNVESGFEDYAFDDMSANLGDEALSILHKLQ 191
           +A  +E N+  +    A E L  ++          F D     +S+ L  E+L++L  L 
Sbjct: 135 LARDSEKNRVLIAGHNAREILVRIL----------FADIETTSLSSELVSESLALLVLLH 194

Query: 192 ISESALKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVA---DQVQILSLTA 251
           ++E+  + + S+     +  +T++L + S   R  +  L+  +   A   D   I+S + 
Sbjct: 195 MTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSD 254

Query: 252 DFFIEIVQILKDQI-SKQASKSALKLLIGVCSSSRNRVKAVQAGAVPVLID-LLLDLDSS 311
             F  ++ +LK+ I S++A K  +K +  +C   + R  A+ AGA  +LID L  D D  
Sbjct: 255 SIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRC 314

Query: 312 EKRLCEMILVVLDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILWSVARF 371
           +    E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  
Sbjct: 315 D---TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL--LALC 374

Query: 372 SASQTVIQEMLRLGVVTKLCFLLQVVDAGAKAKEKAKEILKLHGRPWRNSSCLPS 416
           +A +    E    G+VT+L  L+Q  D   +AK KA+ +LKL    W + S + S
Sbjct: 375 TAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDSTVHS 410

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882772.12.4e-21593.16E3 ubiquitin-protein ligase PUB22-like [Benincasa hispida][more]
XP_008441961.11.3e-20387.32PREDICTED: E3 ubiquitin-protein ligase PUB22 [Cucumis melo] >KAA0060143.1 E3 ubi... [more]
XP_023543358.12.5e-19986.32E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo][more]
XP_022978192.15.5e-19985.85E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima][more]
KAG7033684.13.6e-19886.08E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
Q9SVC63.8e-11852.29E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q84TG36.7e-11551.44E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SF154.5e-6336.73E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9LT791.1e-4834.86U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FXA41.6e-4734.70U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
A0A5A7UVZ16.1e-20487.32RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3B4K26.1e-20487.32RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 ... [more]
A0A6J1IKG72.7e-19985.85RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 P... [more]
A0A6J1GGQ87.2e-19785.14RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790... [more]
A0A0A0L1F51.2e-19484.47RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G194780 PE... [more]
Match NameE-valueIdentityDescription
AT3G52450.12.7e-11952.29plant U-box 22 [more]
AT2G35930.14.7e-11651.44plant U-box 23 [more]
AT3G11840.13.2e-6436.73plant U-box 24 [more]
AT3G19380.17.7e-5034.86plant U-box 25 [more]
AT1G49780.11.1e-4834.70plant U-box 26 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 14..78
e-value: 2.7E-32
score: 123.3
IPR003613U box domainPFAMPF04564U-boxcoord: 12..80
e-value: 4.1E-19
score: 68.5
IPR003613U box domainPROSITEPS51698U_BOXcoord: 10..85
score: 40.249702
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 2..87
e-value: 1.1E-26
score: 94.7
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 99..406
e-value: 5.9E-17
score: 63.5
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 8..422
NoneNo IPR availablePANTHERPTHR22849:SF132E3 UBIQUITIN-PROTEIN LIGASE PUB23coord: 8..422
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 15..57
e-value: 2.76983E-20
score: 81.5111
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 3..84
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 103..389

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG04G005140.1ClCG04G005140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity