ClCG04G001160 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG04G001160
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	Eukaryotic aspartyl protease family protein
Location	CG_Chr04: 3551574 .. 3554331 (-)
RNA-Seq Expression	ClCG04G001160
Synteny	ClCG04G001160

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCCCAAGTTAGGTGTGATGTTAAACTCTACACAGCCCCTCATTTCCAAAGTTGGTTTGCCTATCCTTTCTTAAATATTCTTTCCTACTTTCTTAGCTTCTTCCTAGTTCAACTCTCCAATCTCTTCATATTATGCTCTTGTTTTGAAGAAGGGTTCAACAAAACCTAATTCCTTTACTAGCTTTTACCCCTTCTTTTAGTATACTACAATGAGGGTTTGGTTATGTTCATTTGGATATCTGATCCTCTTGGTTAGTGTATTGATTACAACATTATTTATTAATGCATCCGGTTTGAGTTCGAGTTCGAGTTCGAGTTCGAGTGTCTCAAGGCGAGGTCTGCAGAAACCGAATAAGTTGGGTGGTAATGGGTTTAGGGTGAAGCTTAAGCATGTGGATCATGATGGGAAGAACTTGACGAGATTGGAGCGGTTGCGGCGAGGAGTGGTGCGTGGGAAGAGTAGATTGCAAAGGCTAAATGCGAATGGTTCGGTTGGTGAGCAAGTGAAGGCGCCTGTGGTAGCTGGTAATGGTGAGTTTCTTATGAAGTTGGCCATTGGAACTCCACCGAGAAGCTTGTGGGCGATCATGGACACTGGCAGCGATCTGATTTGGACACAATGCAAGCCTTGTGAACAGTGTTTTGATCAAGCAACACCTATTTTTGATCCAACACAATCTTCTTCTTTCTCTAAAATCTCTTGCTCCGATCCCCTCTGTGGAGCTCTCCCCACCTCCACATGCACCAGCCACGGCTGCGAATATTTGTATACCTATGGAGATTCTTCCTCCACCCAAGGTGTTTTGGCTCTTGACACCTTCACATTTGGAGATTCAACTGAACTTCAGGTATTTCCCTCCTCACAAATATATACTCTTTTTGAAATCCGTGAGTGTCCGGACCAGCTTACGCGCACCTCGACTAATCTCACGGGACAACCTAACTGACTTTACAACATTTGTATGTCAAAAAAACCCGTAGGATATTAAATCATCCTAGGTAGTTAGCCACTGTGAATTGAACCCATAACCCCTCTATTATGCCAACCCATGATGGTTTTCCCCCACACGTATAAAACTCATTTTGAAATCATTTCGTCTCCTTCTCAATTATTTGCTTTTAAAAAACTAAACTAAAATTTGAAAACTATGAAACAAAACATATATCATATAGCAATGGTTACCATGATATATGTTTTTTGAAATTTTAAATCTTATTTTTTCAGGGTTTTTTTTTTTTTAATACTATTTTGTTGTTAAAAAAGTTGATAATATTGCTTGAAGTATGTGTAATTAATAGCGTGAGACGTACGAGGATCATTGGGTTGATTGAGGGTGGTTGTTGGGTGTAGGTGAATTTTAGATAATAAATAATACCAATAATTAAAATTGTTTGTTTCAAAAACTTTAAGGAGACTAAAACATAATATTAATTTTCAAAATTTATTGAACAAAATGTTTTTTCTTTTAAGTTCAAATGCATCAAAATAAATATTTTGAAAATTTGGGGGAAGATTTAATATATATACACACACAAGTTTTTAAAATATACTAGGGTTTTTATGTATTTCTTATAAAATTGAAGCAAAAGTAACTTTTTCTTAATTAGTTTTCGAAAACTTGATTGGTTTTTATAAACAATGGTAAAAAACGGATAACAAAATAAAGAAACTATAAATATAAGTAATGCTTATAATAAACTAAAAGATTCAAAAAATTTAAAACAAAAAAAAATGAGATCTAAATGAAATATATTTAATTTTTTATACATAATCTTAATCCAGGTCTCAATCTCCGGACTCGGGTTTGGATGCGGAGACGATAACGAAGGAGATGGGTTTAGCCAAGGCGCGGGGTTGGTGGGGCTCGGGCGAGGACCCTTATCGTTGGTTTCTCAATTAAAAGAACAAAAGTTTGCTTATTGTTTAACAGCCATTGATGAGTCGAAACCAAGCTCACTTTTGTTGGGATCTCTAGCAAACATAAATCCTAAAACATCAAAAGATGAATTGAAAACAACCCCATTGATCAGAAACCCTTCTCAGCCATCTTTTTACTATCTTTCTCTCCAAGGAATATCAGTTGGTGACACTCAATTATCAATACCAAAGTCCACTTTTGAGCTCCATAATGATGGAAGTGGCGGAGTAATCATAGACTCAGGCACAACAATCACTTACATTGAGAACACAGCTTTTACTTTACTCAAAAATGAGTTCATTGCTCAAATGAGCCTTCCCGTCGACGACTCCGGTACCGGTGGCCTTGACCTCTGCTTTAACCTACCAGCCGAGGCAACTCAGGTATGTTTACCTAAAAACTTTAAGTTGGTAGTATTTTGAGAGTGATTTTGAAAAAGTTAAAATGATTCCAGTCATATTTAAAATTTTTTCAAAACACACTTTTAATTACTCAAAATTTAATTTTTTATTTTTAAGCATAATTTGTTTACAACAATGAAAAGCATAACAATTTTAGAATCACTCTCAAACTTGTCATTAATTATTTTATTTAATTATGTTTTCTTAACACAGGTGGAGGTTCCGAAGTTGACGTTTCATTTCAAGGGCGCTGATTTGGAGCTTCCCGGGGAGAACTACATGATCGGCGATTCGAGGGCAGGATTGATATGCTTGGCCATTGGGAGTTCTAGAGGAATGTCCATCTTCGGAAATCTTCAGCAACAAAACTTCATGGTTGTTCATGATCTTCAGGAAGAAACCCTATCATTTTTGCCCACTCAATGTGATAGTATATAA

mRNA sequence

TTCCCAAGTTAGGTGTGATGTTAAACTCTACACAGCCCCTCATTTCCAAAGTTGGTTTGCCTATCCTTTCTTAAATATTCTTTCCTACTTTCTTAGCTTCTTCCTAGTTCAACTCTCCAATCTCTTCATATTATGCTCTTGTTTTGAAGAAGGGTTCAACAAAACCTAATTCCTTTACTAGCTTTTACCCCTTCTTTTAGTATACTACAATGAGGGTTTGGTTATGTTCATTTGGATATCTGATCCTCTTGGTTAGTGTATTGATTACAACATTATTTATTAATGCATCCGGTTTGAGTTCGAGTTCGAGTTCGAGTTCGAGTGTCTCAAGGCGAGGTCTGCAGAAACCGAATAAGTTGGGTGGTAATGGGTTTAGGGTGAAGCTTAAGCATGTGGATCATGATGGGAAGAACTTGACGAGATTGGAGCGGTTGCGGCGAGGAGTGGTGCGTGGGAAGAGTAGATTGCAAAGGCTAAATGCGAATGGTTCGGTTGGTGAGCAAGTGAAGGCGCCTGTGGTAGCTGGTAATGGTGAGTTTCTTATGAAGTTGGCCATTGGAACTCCACCGAGAAGCTTGTGGGCGATCATGGACACTGGCAGCGATCTGATTTGGACACAATGCAAGCCTTGTGAACAGTGTTTTGATCAAGCAACACCTATTTTTGATCCAACACAATCTTCTTCTTTCTCTAAAATCTCTTGCTCCGATCCCCTCTGTGGAGCTCTCCCCACCTCCACATGCACCAGCCACGGCTGCGAATATTTGTATACCTATGGAGATTCTTCCTCCACCCAAGGTGTTTTGGCTCTTGACACCTTCACATTTGGAGATTCAACTGAACTTCAGGTCTCAATCTCCGGACTCGGGTTTGGATGCGGAGACGATAACGAAGGAGATGGGTTTAGCCAAGGCGCGGGGTTGGTGGGGCTCGGGCGAGGACCCTTATCGTTGGTTTCTCAATTAAAAGAACAAAAGTTTGCTTATTGTTTAACAGCCATTGATGAGTCGAAACCAAGCTCACTTTTGTTGGGATCTCTAGCAAACATAAATCCTAAAACATCAAAAGATGAATTGAAAACAACCCCATTGATCAGAAACCCTTCTCAGCCATCTTTTTACTATCTTTCTCTCCAAGGAATATCAGTTGGTGACACTCAATTATCAATACCAAAGTCCACTTTTGAGCTCCATAATGATGGAAGTGGCGGAGTAATCATAGACTCAGGCACAACAATCACTTACATTGAGAACACAGCTTTTACTTTACTCAAAAATGAGTTCATTGCTCAAATGAGCCTTCCCGTCGACGACTCCGGTACCGGTGGCCTTGACCTCTGCTTTAACCTACCAGCCGAGGCAACTCAGGTGGAGGTTCCGAAGTTGACGTTTCATTTCAAGGGCGCTGATTTGGAGCTTCCCGGGGAGAACTACATGATCGGCGATTCGAGGGCAGGATTGATATGCTTGGCCATTGGGAGTTCTAGAGGAATGTCCATCTTCGGAAATCTTCAGCAACAAAACTTCATGGTTGTTCATGATCTTCAGGAAGAAACCCTATCATTTTTGCCCACTCAATGTGATAGTATATAA

Coding sequence (CDS)

ATGAGGGTTTGGTTATGTTCATTTGGATATCTGATCCTCTTGGTTAGTGTATTGATTACAACATTATTTATTAATGCATCCGGTTTGAGTTCGAGTTCGAGTTCGAGTTCGAGTGTCTCAAGGCGAGGTCTGCAGAAACCGAATAAGTTGGGTGGTAATGGGTTTAGGGTGAAGCTTAAGCATGTGGATCATGATGGGAAGAACTTGACGAGATTGGAGCGGTTGCGGCGAGGAGTGGTGCGTGGGAAGAGTAGATTGCAAAGGCTAAATGCGAATGGTTCGGTTGGTGAGCAAGTGAAGGCGCCTGTGGTAGCTGGTAATGGTGAGTTTCTTATGAAGTTGGCCATTGGAACTCCACCGAGAAGCTTGTGGGCGATCATGGACACTGGCAGCGATCTGATTTGGACACAATGCAAGCCTTGTGAACAGTGTTTTGATCAAGCAACACCTATTTTTGATCCAACACAATCTTCTTCTTTCTCTAAAATCTCTTGCTCCGATCCCCTCTGTGGAGCTCTCCCCACCTCCACATGCACCAGCCACGGCTGCGAATATTTGTATACCTATGGAGATTCTTCCTCCACCCAAGGTGTTTTGGCTCTTGACACCTTCACATTTGGAGATTCAACTGAACTTCAGGTCTCAATCTCCGGACTCGGGTTTGGATGCGGAGACGATAACGAAGGAGATGGGTTTAGCCAAGGCGCGGGGTTGGTGGGGCTCGGGCGAGGACCCTTATCGTTGGTTTCTCAATTAAAAGAACAAAAGTTTGCTTATTGTTTAACAGCCATTGATGAGTCGAAACCAAGCTCACTTTTGTTGGGATCTCTAGCAAACATAAATCCTAAAACATCAAAAGATGAATTGAAAACAACCCCATTGATCAGAAACCCTTCTCAGCCATCTTTTTACTATCTTTCTCTCCAAGGAATATCAGTTGGTGACACTCAATTATCAATACCAAAGTCCACTTTTGAGCTCCATAATGATGGAAGTGGCGGAGTAATCATAGACTCAGGCACAACAATCACTTACATTGAGAACACAGCTTTTACTTTACTCAAAAATGAGTTCATTGCTCAAATGAGCCTTCCCGTCGACGACTCCGGTACCGGTGGCCTTGACCTCTGCTTTAACCTACCAGCCGAGGCAACTCAGGTGGAGGTTCCGAAGTTGACGTTTCATTTCAAGGGCGCTGATTTGGAGCTTCCCGGGGAGAACTACATGATCGGCGATTCGAGGGCAGGATTGATATGCTTGGCCATTGGGAGTTCTAGAGGAATGTCCATCTTCGGAAATCTTCAGCAACAAAACTTCATGGTTGTTCATGATCTTCAGGAAGAAACCCTATCATTTTTGCCCACTCAATGTGATAGTATATAA

Protein sequence

MRVWLCSFGYLILLVSVLITTLFINASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLKHVDHDGKNLTRLERLRRGVVRGKSRLQRLNANGSVGEQVKAPVVAGNGEFLMKLAIGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPTSTCTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSLLLGSLANINPKTSKDELKTTPLIRNPSQPSFYYLSLQGISVGDTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMSLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI

Homology

[Close]

BLAST of ClCG04G001160 vs. NCBI nr
Match: XP_038883313.1 (aspartic proteinase nepenthesin-1 [Benincasa hispida])

HSP 1 Score: 765.4 bits (1975), Expect = 2.8e-217
Identity = 397/465 (85.38%), Postives = 418/465 (89.89%), Query Frame = 0


Query: 1   MRVWLCSFGYLILLVSVLITTLFINASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLK 60
           M V L S+  LILLV VLITTLFI+   L          SRR LQK N+L  NGF+VKL 
Sbjct: 1   MTVSLHSYRCLILLVIVLITTLFIDTLAL----------SRRALQKSNELPSNGFKVKLN 60

Query: 61  HVDHDGKNLTRLERLRRGVVRGKSRLQRLN-----ANGSVGEQVKAPVVAGNGEFLMKLA 120
           HVDH  KNLTR ERLRRGV RGK+RL RLN     AN +VGEQV+APVVAGNGEFLMKLA
Sbjct: 61  HVDH-VKNLTRFERLRRGVARGKNRLHRLNAMVLAANAAVGEQVQAPVVAGNGEFLMKLA 120

Query: 121 IGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPT 180
           IGTPP+S  AIMDTGSDLIWTQCKPC+QCFDQATPIFDP  SSSFSKISCS  LCG LPT
Sbjct: 121 IGTPPKSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKASSSFSKISCSSELCGPLPT 180

Query: 181 STCTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQG 240
           STC+S GCEYLYTYGDSSSTQGVLAL+TFTFGDS++ QVSI+GLGFGCGDDNEGDGFSQG
Sbjct: 181 STCSSDGCEYLYTYGDSSSTQGVLALETFTFGDSSDDQVSITGLGFGCGDDNEGDGFSQG 240

Query: 241 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSLLLGSLANINPKTSKDELKTTPLI 300
           AGLVGLGRGPLSLVSQLKEQKFAYCLTAID+SKPSSLLLGSLANI PKT+KDE+KTTPLI
Sbjct: 241 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLI 300

Query: 301 RNPSQPSFYYLSLQGISVGDTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLK 360
           RNPSQPSFYYLSLQGISVG TQLSIPK+TFELH+DGSGGVIIDSGTTITYIENTAFTLLK
Sbjct: 301 RNPSQPSFYYLSLQGISVGSTQLSIPKTTFELHDDGSGGVIIDSGTTITYIENTAFTLLK 360

Query: 361 NEFIAQMSLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRA 420
           NEFIAQM+LPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHF+GADLELPGENYMIGDSR 
Sbjct: 361 NEFIAQMNLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFEGADLELPGENYMIGDSRT 420

Query: 421 GLICLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461
           GLICLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 421 GLICLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 454

[Close]

BLAST of ClCG04G001160 vs. NCBI nr
Match: XP_011653928.1 (aspartic proteinase nepenthesin-1 [Cucumis sativus] >KGN54860.1 hypothetical protein Csa_012038 [Cucumis sativus])

HSP 1 Score: 760.8 bits (1963), Expect = 7.0e-216
Identity = 394/465 (84.73%), Postives = 420/465 (90.32%), Query Frame = 0


Query: 3   VWLCSFGYL-ILLVSVLITTLFINASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLKH 62
           V L SFGYL  LL+ +LITTLFIN      + + SSS+SRR LQKPNKL  +GFRV+LKH
Sbjct: 4   VSLRSFGYLHRLLLIILITTLFIN------TLAFSSSLSRRALQKPNKLPSHGFRVRLKH 63

Query: 63  VDHDGKNLTRLERLRRGVVRGKSRLQRLN------ANGSVGEQVKAPVVAGNGEFLMKLA 122
           VDH  KNLTR ERLRRGV RGK+RL RLN      AN +VG+QVKAPVVAGNGEFLMKLA
Sbjct: 64  VDH-VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLA 123

Query: 123 IGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPT 182
           IG+PPRS  AIMDTGSDLIWTQCKPC+QCFDQ+TPIFDP QSSSF KISCS  LCGALPT
Sbjct: 124 IGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPKQSSSFYKISCSSELCGALPT 183

Query: 183 STCTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQG 242
           STC+S GCEYLYTYGDSSSTQGVLA +TFTFGDSTE Q+SI GLGFGCG+DN GDGFSQG
Sbjct: 184 STCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQG 243

Query: 243 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSLLLGSLANINPKTSKDELKTTPLI 302
           AGLVGLGRGPLSLVSQLKEQKFAYCLTAID+SKPSSLLLGSLANI PKTSKDE+KTTPLI
Sbjct: 244 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLI 303

Query: 303 RNPSQPSFYYLSLQGISVGDTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLK 362
           +NPSQPSFYYLSLQGISVG TQLSIPKSTFELH+DGSGGVIIDSGTTITY+EN+AFT LK
Sbjct: 304 KNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYVENSAFTSLK 363

Query: 363 NEFIAQMSLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRA 422
           NEFIAQM+LPVDDSGTGGLDLCFNLPA   QVEVPKLTFHFKGADLELPGENYMIGDS+A
Sbjct: 364 NEFIAQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKA 423

Query: 423 GLICLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461
           GL+CLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 424 GLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of ClCG04G001160 vs. NCBI nr
Match: TYK02448.1 (aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa])

HSP 1 Score: 758.8 bits (1958), Expect = 2.7e-215
Identity = 394/465 (84.73%), Postives = 418/465 (89.89%), Query Frame = 0


Query: 3   VWLCSFGYL-ILLVSVLITTLFINASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLKH 62
           V L SFGYL +LL+ V ITTLFIN      + + SSS+SRR LQKPNKL  +GF V+LKH
Sbjct: 4   VSLRSFGYLQLLLLIVFITTLFIN------TLAFSSSLSRRALQKPNKLPSHGFMVRLKH 63

Query: 63  VDHDGKNLTRLERLRRGVVRGKSRLQRLN------ANGSVGEQVKAPVVAGNGEFLMKLA 122
           VDH  KNLTR ERLRRGV RGK+RL RLN      AN SVG+QVKAPVVAGNGEFLMKLA
Sbjct: 64  VDH-VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLA 123

Query: 123 IGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPT 182
           IG+PPRS  AIMDTGSDLIWTQCKPC+QCFDQATPIFDP QSSSFSKISC   LCGALPT
Sbjct: 124 IGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPT 183

Query: 183 STCTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQG 242
           STC+S GCEYLYTYGDSSSTQGVLA +TFTFGDSTE Q+SI GLGFGCG+DN GDGFSQG
Sbjct: 184 STCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQG 243

Query: 243 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSLLLGSLANINPKTSKDELKTTPLI 302
           AGLVGLGRGPLSLVSQLKEQKFAYCLTAID+SKPSSLLLGSLANI PKTSKDE+K TPLI
Sbjct: 244 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLI 303

Query: 303 RNPSQPSFYYLSLQGISVGDTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLK 362
           +NPSQPSFYYLSLQGISVG TQLSIPKSTFELH+DGSGGVIIDSGTTITYIE+TAF+ LK
Sbjct: 304 KNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLK 363

Query: 363 NEFIAQMSLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRA 422
           NEFIAQM+LPVDDSGTGGLDLCFNLPA  TQVEVPKLTFHFKGADLELPGENYMIGDS+ 
Sbjct: 364 NEFIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKT 423

Query: 423 GLICLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461
           GL+CLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 424 GLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of ClCG04G001160 vs. NCBI nr
Match: XP_008442220.1 (PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo] >KAA0041170.1 aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa])

HSP 1 Score: 758.8 bits (1958), Expect = 2.7e-215
Identity = 394/465 (84.73%), Postives = 418/465 (89.89%), Query Frame = 0


Query: 3   VWLCSFGYL-ILLVSVLITTLFINASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLKH 62
           V L SFGYL +LL+ V ITTLFIN      + + SSS+S R LQKPNKL  +GFRV+LKH
Sbjct: 4   VSLRSFGYLQLLLLIVFITTLFIN------TLAFSSSLSTRALQKPNKLPSHGFRVRLKH 63

Query: 63  VDHDGKNLTRLERLRRGVVRGKSRLQRLN------ANGSVGEQVKAPVVAGNGEFLMKLA 122
           VDH  KNLTR ERLRRGV RGK+RL RLN      AN SVG+QVKAPVVAGNGEFLMKLA
Sbjct: 64  VDH-VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLA 123

Query: 123 IGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPT 182
           IG+PPRS  AIMDTGSDLIWTQCKPC+QCFDQATPIFDP QSSSFSKISC   LCGALPT
Sbjct: 124 IGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPT 183

Query: 183 STCTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQG 242
           STC+S GCEYLYTYGDSSSTQGVLA +TFTFGDSTE Q+SI GLGFGCG+DN GDGFSQG
Sbjct: 184 STCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQG 243

Query: 243 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSLLLGSLANINPKTSKDELKTTPLI 302
           AGLVGLGRGPLSLVSQLKEQKFAYCLTAID+SKPSSLLLGSLANI PKTSKDE+K TPLI
Sbjct: 244 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLI 303

Query: 303 RNPSQPSFYYLSLQGISVGDTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLK 362
           +NPSQPSFYYLSLQGISVG TQLSIPKSTFELH+DGSGGVIIDSGTTITYIE+TAF+ LK
Sbjct: 304 KNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLK 363

Query: 363 NEFIAQMSLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRA 422
           NEFIAQM+LPVDDSGTGGLDLCFNLPA  TQVEVPKLTFHFKGADLELPGENYMIGDS+ 
Sbjct: 364 NEFIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKT 423

Query: 423 GLICLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461
           GL+CLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 424 GLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of ClCG04G001160 vs. NCBI nr
Match: XP_022154910.1 (aspartic proteinase nepenthesin-1 [Momordica charantia])

HSP 1 Score: 701.0 bits (1808), Expect = 6.6e-198
Identity = 357/463 (77.11%), Postives = 399/463 (86.18%), Query Frame = 0


Query: 5   LCSFGYLILLVSVLITTLFINASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLKHVDH 64
           LCS  Y I+LV+VL T  FI+       + SSS++SRR LQ+  KL  NGFR++L HVDH
Sbjct: 5   LCSVRYPIVLVAVLATLFFIDL------TVSSSTLSRRALQQ-QKLLNNGFRMRLHHVDH 64

Query: 65  DGKNLTRLERLRRGVVRGKSRLQRLNA-------NGSVGEQVKAPVVAGNGEFLMKLAIG 124
             KNLTR ERL+RG  RG++RLQRLNA         +VG+QV+APVVAGNGEFLMKLAIG
Sbjct: 65  HVKNLTRFERLQRGAARGRNRLQRLNAMVLAAAGGAAVGDQVQAPVVAGNGEFLMKLAIG 124

Query: 125 TPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPTST 184
           +PP+S  AIMDTGSDLIWTQCKPC+QCFDQ+TPIFDP +SSSFSK+SCS  LCGALPTS 
Sbjct: 125 SPPKSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPKESSSFSKLSCSSELCGALPTSA 184

Query: 185 CTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQGAG 244
           C++ GCEYLYTYGD SST G+L  +TFTFGD  E QVSIS +GFGCGDDNEGDGFSQGAG
Sbjct: 185 CSNDGCEYLYTYGDYSSTHGLLGAETFTFGDPGEDQVSISEIGFGCGDDNEGDGFSQGAG 244

Query: 245 LVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSLLLGSLANINPKTSKDELKTTPLIRN 304
           LVGLGRGPLSLVSQLKEQKFAYCLT ID+SKPSSLL+GSLAN+ PK S+DE+KTTPLIRN
Sbjct: 245 LVGLGRGPLSLVSQLKEQKFAYCLTPIDDSKPSSLLMGSLANVKPKKSQDEIKTTPLIRN 304

Query: 305 PSQPSFYYLSLQGISVGDTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNE 364
           PSQPSFYYLSL+GISVG + L+IP+ TFEL +DGSGGVIIDSGTTITYI+ +AFTLLK E
Sbjct: 305 PSQPSFYYLSLEGISVGGSHLAIPQPTFELRDDGSGGVIIDSGTTITYIDKSAFTLLKKE 364

Query: 365 FIAQMSLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGL 424
           FIAQM LPVDDSGTGGLDLCF LP+EATQVEVPKLTFHFK ADLELPGENYMIGDS AGL
Sbjct: 365 FIAQMKLPVDDSGTGGLDLCFKLPSEATQVEVPKLTFHFKDADLELPGENYMIGDSAAGL 424

Query: 425 ICLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461
           +CLAIGSS GMSIFGNLQQQNFMVVHDLQEET+SF+PTQCD I
Sbjct: 425 LCLAIGSSSGMSIFGNLQQQNFMVVHDLQEETISFVPTQCDGI 460

[Close]

BLAST of ClCG04G001160 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 3.8e-116
Identity = 224/424 (52.83%), Postives = 290/424 (68.40%), Query Frame = 0


Query: 38  SVSRRGLQKPNKLGGNGFRVKLKHVDHDGKNLTRLERLRRGVVRGKSRLQRLNA--NGSV 97
           S SR  L   ++    GF++ L+HVD  GKNLT+ + L R + RG  RLQRL A  NG  
Sbjct: 24  STSRTALNHRHEAKVTGFQIMLEHVD-SGKNLTKFQLLERAIERGSRRLQRLEAMLNGPS 83

Query: 98  GEQVKAPVVAGNGEFLMKLAIGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDPT 157
           G  V+  V AG+GE+LM L+IGTP +   AIMDTGSDLIWTQC+PC QCF+Q+TPIF+P 
Sbjct: 84  G--VETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQ 143

Query: 158 QSSSFSKISCSDPLCGALPTSTCTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQVS 217
            SSSFS + CS  LC AL + TC+++ C+Y Y YGD S TQG +  +T TFG      VS
Sbjct: 144 GSSSFSTLPCSSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFG-----SVS 203

Query: 218 ISGLGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSLLLG 277
           I  + FGCG++N+G G   GAGLVG+GRGPLSL SQL   KF+YC+T I  S PS+LLLG
Sbjct: 204 IPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLG 263

Query: 278 SLANINPKTSKDELKTTPLIRNPSQPSFYYLSLQGISVGDTQLSIPKSTFELH-NDGSGG 337
           SLAN     S +    T LI++   P+FYY++L G+SVG T+L I  S F L+ N+G+GG
Sbjct: 264 SLANSVTAGSPN----TTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGG 323

Query: 338 VIIDSGTTITYIENTAFTLLKNEFIAQMSLPVDDSGTGGLDLCFNLPAEATQVEVPKLTF 397
           +IIDSGTT+TY  N A+  ++ EFI+Q++LPV +  + G DLCF  P++ + +++P    
Sbjct: 324 IIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVM 383

Query: 398 HFKGADLELPGENYMIGDSRAGLICLAIG-SSRGMSIFGNLQQQNFMVVHDLQEETLSFL 457
           HF G DLELP ENY I  S  GLICLA+G SS+GMSIFGN+QQQN +VV+D     +SF 
Sbjct: 384 HFDGGDLELPSENYFISPSN-GLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFA 434

[Close]

BLAST of ClCG04G001160 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 2.3e-108
Identity = 209/446 (46.86%), Postives = 292/446 (65.47%), Query Frame = 0


Query: 14  LVSVLITTLFINASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLKHVDHDGKNLTRLE 73
           L SV++    ++A    +SS+S  ++   G ++P      G RV L+ VD  GKNLT+ E
Sbjct: 5   LYSVVLGLAIVSAIVAPTSSTSRGTLLHHGQKRPQP----GLRVDLEQVD-SGKNLTKYE 64

Query: 74  RLRRGVVRGKSRLQRLNANGSVGEQVKAPVVAGNGEFLMKLAIGTPPRSLWAIMDTGSDL 133
            ++R + RG+ R++ +NA       ++ PV AG+GE+LM +AIGTP  S  AIMDTGSDL
Sbjct: 65  LIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDL 124

Query: 134 IWTQCKPCEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPTSTCTSHGCEYLYTYGDSS 193
           IWTQC+PC QCF Q TPIF+P  SSSFS + C    C  LP+ TC ++ C+Y Y YGD S
Sbjct: 125 IWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYGDGS 184

Query: 194 STQGVLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLK 253
           +TQG +A +TFTF  S     S+  + FGCG+DN+G G   GAGL+G+G GPLSL SQL 
Sbjct: 185 TTQGYMATETFTFETS-----SVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLG 244

Query: 254 EQKFAYCLTAIDESKPSSLLLGSLANINPKTSKDELKTTPLIRNPSQPSFYYLSLQGISV 313
             +F+YC+T+   S PS+L LGS A+  P+ S     +T LI +   P++YY++LQGI+V
Sbjct: 245 VGQFSYCMTSYGSSSPSTLALGSAASGVPEGS----PSTTLIHSSLNPTYYYITLQGITV 304

Query: 314 GDTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMSLPVDDSGTGG 373
           G   L IP STF+L +DG+GG+IIDSGTT+TY+   A+  +   F  Q++LP  D  + G
Sbjct: 305 GGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSG 364

Query: 374 LDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSR--GMSIF 433
           L  CF  P++ + V+VP+++  F G  L L  +N +I  +  G+ICLA+GSS   G+SIF
Sbjct: 365 LSTCFQQPSDGSTVQVPEISMQFDGGVLNLGEQNILISPAE-GVICLAMGSSSQLGISIF 424

Query: 434 GNLQQQNFMVVHDLQEETLSFLPTQC 458
           GN+QQQ   V++DLQ   +SF+PTQC
Sbjct: 425 GNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of ClCG04G001160 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 2.4e-70
Identity = 143/368 (38.86%), Postives = 215/368 (58.42%), Query Frame = 0


Query: 97  EQVKAPVVA----GNGEFLMKLAIGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIF 156
           E +  PVV+    G+GE+  ++ +GTP + ++ ++DTGSD+ W QC+PC  C+ Q+ P+F
Sbjct: 145 EDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVF 204

Query: 157 DPTQSSSFSKISCSDPLCGALPTSTCTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTEL 216
           +PT SS++  ++CS P C  L TS C S+ C Y  +YGD S T G LA DT TFG+S + 
Sbjct: 205 NPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGK- 264

Query: 217 QVSISGLGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSL 276
              I+ +  GCG DNEG  F+  AGL+GLG G LS+ +Q+K   F+YCL   D  K SSL
Sbjct: 265 ---INNVALGCGHDNEG-LFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSL 324

Query: 277 LLGSLANINPKTSKDELKTTPLIRNPSQPSFYYLSLQGISVGDTQLSIPKSTFELHNDGS 336
                 + N         T PL+RN    +FYY+ L G SVG  ++ +P + F++   GS
Sbjct: 325 ------DFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGS 384

Query: 337 GGVIIDSGTTITYIENTAFTLLKNEFI-AQMSLPVDDSGTGGLDLCFNLPAEATQVEVPK 396
           GGVI+D GT +T ++  A+  L++ F+   ++L    S     D C++  + +T V+VP 
Sbjct: 385 GGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST-VKVPT 444

Query: 397 LTFHFKGA-DLELPGENYMIGDSRAGLICLAIG-SSRGMSIFGNLQQQNFMVVHDLQEET 456
           + FHF G   L+LP +NY+I    +G  C A   +S  +SI GN+QQQ   + +DL +  
Sbjct: 445 VAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNV 500

Query: 457 LSFLPTQC 458
           +     +C
Sbjct: 505 IGLSGNKC 500

[Close]

BLAST of ClCG04G001160 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.5e-67
Identity = 159/443 (35.89%), Postives = 235/443 (53.05%), Query Frame = 0


Query: 26  ASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLKHVDHDGKNL-----TRLERLRRGVV 85
           AS  SS   S   +S   L   N     GF   L H D           T  +RLR  + 
Sbjct: 2   ASLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIH 61

Query: 86  RGKSRLQRLNANGSVGEQVKAPVVAGNGEFLMKLAIGTPPRSLWAIMDTGSDLIWTQCKP 145
           R  +R+       +   Q +  + + +GE+LM ++IGTPP  + AI DTGSDL+WTQC P
Sbjct: 62  RSVNRVFHFTEKDNT-PQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAP 121

Query: 146 CEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPT-STCTSHG--CEYLYTYGDSSSTQG 205
           C+ C+ Q  P+FDP  SS++  +SCS   C AL   ++C+++   C Y  +YGD+S T+G
Sbjct: 122 CDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKG 181

Query: 206 VLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQ-- 265
            +A+DT T G S    + +  +  GCG +N G    +G+G+VGLG GP+SL+ QL +   
Sbjct: 182 NIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSID 241

Query: 266 -KFAYCLTAIDESKPSSLLLGSLANINPKTSKDELKTTPLIRNPSQPSFYYLSLQGISVG 325
            KF+YCL  +   K  +  +      N   S   + +TPLI   SQ +FYYL+L+ ISVG
Sbjct: 242 GKFSYCLVPLTSKKDQTSKIN--FGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVG 301

Query: 326 DTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMSLPVDDSGTGGL 385
             Q+    S  E      G +IIDSGTT+T +    ++ L++   + +          GL
Sbjct: 302 SKQIQYSGSDSE---SSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGL 361

Query: 386 DLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNL 445
            LC++   +   ++VP +T HF GAD++L   N  +  S   L+C A   S   SI+GN+
Sbjct: 362 SLCYSATGD---LKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGNV 421

Query: 446 QQQNFMVVHDLQEETLSFLPTQC 458
            Q NF+V +D   +T+SF PT C
Sbjct: 422 AQMNFLVGYDTVSKTVSFKPTDC 434

[Close]

BLAST of ClCG04G001160 vs. ExPASy Swiss-Prot
Match: Q7XV21 (Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2)

HSP 1 Score: 256.5 bits (654), Expect = 5.6e-67
Identity = 157/446 (35.20%), Postives = 232/446 (52.02%), Query Frame = 0


Query: 55  FRVKLKHVD---HDGKNLTRLERLRRGVVRGKSRLQRLN-ANGSVGEQVKA-----PVVA 114
           FR++L  VD    D  NLT  E LRR + R + RL  +  A G      KA     P++ 
Sbjct: 25  FRLELASVDASAADAANLTEHELLRRAIQRSRYRLAGIGMARGEAASARKAVVAETPIMP 84

Query: 115 GNGEFLMKLAIGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDPTQSSSFSKISC 174
             GE+L+KL IGTPP    A +DT SDLIWTQC+PC  C+ Q  P+F+P  SS+++ + C
Sbjct: 85  AGGEYLVKLGIGTPPYKFTAAIDTASDLIWTQCQPCTGCYHQVDPMFNPRVSSTYAALPC 144

Query: 175 SDPLCGALPTSTC---TSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQVSISGLGFG 234
           S   C  L    C       C+Y YTY  +++T+G LA+D    G+      +  G+ FG
Sbjct: 145 SSDTCDELDVHRCGHDDDESCQYTYTYSGNATTEGTLAVDKLVIGED-----AFRGVAFG 204

Query: 235 CGDDNEGDG-FSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSLLLGSLANIN 294
           C   + G     Q +G+VGLGRGPLSLVSQL  ++FAYCL       P  L+LG  A+ +
Sbjct: 205 CSTSSTGGAPPPQASGVVGLGRGPLSLVSQLSVRRFAYCLPPPASRIPGKLVLG--ADAD 264

Query: 295 PKTSKDELKTTPLIRNPSQPSFYYLSLQGISVGDTQLSIPKST----------------- 354
              +       P+ R+P  PS+YYL+L G+ +GD  +S+P +T                 
Sbjct: 265 AARNATNRIAVPMRRDPRYPSYYYLNLDGLLIGDRAMSLPPTTTTTATATATAPAPAPTP 324

Query: 355 ------FELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMSLPVDDSGTGGLDLCF 414
                   + +    G+IID  +TIT++E + +  L N+   ++ LP     + GLDLCF
Sbjct: 325 SPNATAVAVGDANRYGMIIDIASTITFLEASLYDELVNDLEVEIRLPRGTGSSLGLDLCF 384

Query: 415 NLP--AEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSR--GMSIFGNL 461
            LP      +V VP +   F G  L L        D  +G++CL +G +    +SI GN 
Sbjct: 385 ILPDGVAFDRVYVPAVALAFDGRWLRLDKARLFAEDRESGMMCLMVGRAEAGSVSILGNF 444

[Close]

BLAST of ClCG04G001160 vs. ExPASy TrEMBL
Match: A0A0A0KYT9 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G554680 PE=3 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 3.4e-216
Identity = 394/465 (84.73%), Postives = 420/465 (90.32%), Query Frame = 0


Query: 3   VWLCSFGYL-ILLVSVLITTLFINASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLKH 62
           V L SFGYL  LL+ +LITTLFIN      + + SSS+SRR LQKPNKL  +GFRV+LKH
Sbjct: 4   VSLRSFGYLHRLLLIILITTLFIN------TLAFSSSLSRRALQKPNKLPSHGFRVRLKH 63

Query: 63  VDHDGKNLTRLERLRRGVVRGKSRLQRLN------ANGSVGEQVKAPVVAGNGEFLMKLA 122
           VDH  KNLTR ERLRRGV RGK+RL RLN      AN +VG+QVKAPVVAGNGEFLMKLA
Sbjct: 64  VDH-VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLA 123

Query: 123 IGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPT 182
           IG+PPRS  AIMDTGSDLIWTQCKPC+QCFDQ+TPIFDP QSSSF KISCS  LCGALPT
Sbjct: 124 IGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPKQSSSFYKISCSSELCGALPT 183

Query: 183 STCTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQG 242
           STC+S GCEYLYTYGDSSSTQGVLA +TFTFGDSTE Q+SI GLGFGCG+DN GDGFSQG
Sbjct: 184 STCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQG 243

Query: 243 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSLLLGSLANINPKTSKDELKTTPLI 302
           AGLVGLGRGPLSLVSQLKEQKFAYCLTAID+SKPSSLLLGSLANI PKTSKDE+KTTPLI
Sbjct: 244 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLI 303

Query: 303 RNPSQPSFYYLSLQGISVGDTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLK 362
           +NPSQPSFYYLSLQGISVG TQLSIPKSTFELH+DGSGGVIIDSGTTITY+EN+AFT LK
Sbjct: 304 KNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYVENSAFTSLK 363

Query: 363 NEFIAQMSLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRA 422
           NEFIAQM+LPVDDSGTGGLDLCFNLPA   QVEVPKLTFHFKGADLELPGENYMIGDS+A
Sbjct: 364 NEFIAQMNLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKA 423

Query: 423 GLICLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461
           GL+CLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 424 GLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of ClCG04G001160 vs. ExPASy TrEMBL
Match: A0A5A7TD10 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold128G001020 PE=3 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.3e-215
Identity = 394/465 (84.73%), Postives = 418/465 (89.89%), Query Frame = 0


Query: 3   VWLCSFGYL-ILLVSVLITTLFINASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLKH 62
           V L SFGYL +LL+ V ITTLFIN      + + SSS+S R LQKPNKL  +GFRV+LKH
Sbjct: 4   VSLRSFGYLQLLLLIVFITTLFIN------TLAFSSSLSTRALQKPNKLPSHGFRVRLKH 63

Query: 63  VDHDGKNLTRLERLRRGVVRGKSRLQRLN------ANGSVGEQVKAPVVAGNGEFLMKLA 122
           VDH  KNLTR ERLRRGV RGK+RL RLN      AN SVG+QVKAPVVAGNGEFLMKLA
Sbjct: 64  VDH-VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLA 123

Query: 123 IGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPT 182
           IG+PPRS  AIMDTGSDLIWTQCKPC+QCFDQATPIFDP QSSSFSKISC   LCGALPT
Sbjct: 124 IGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPT 183

Query: 183 STCTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQG 242
           STC+S GCEYLYTYGDSSSTQGVLA +TFTFGDSTE Q+SI GLGFGCG+DN GDGFSQG
Sbjct: 184 STCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQG 243

Query: 243 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSLLLGSLANINPKTSKDELKTTPLI 302
           AGLVGLGRGPLSLVSQLKEQKFAYCLTAID+SKPSSLLLGSLANI PKTSKDE+K TPLI
Sbjct: 244 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLI 303

Query: 303 RNPSQPSFYYLSLQGISVGDTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLK 362
           +NPSQPSFYYLSLQGISVG TQLSIPKSTFELH+DGSGGVIIDSGTTITYIE+TAF+ LK
Sbjct: 304 KNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLK 363

Query: 363 NEFIAQMSLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRA 422
           NEFIAQM+LPVDDSGTGGLDLCFNLPA  TQVEVPKLTFHFKGADLELPGENYMIGDS+ 
Sbjct: 364 NEFIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKT 423

Query: 423 GLICLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461
           GL+CLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 424 GLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of ClCG04G001160 vs. ExPASy TrEMBL
Match: A0A5D3BTY9 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1738G00580 PE=3 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.3e-215
Identity = 394/465 (84.73%), Postives = 418/465 (89.89%), Query Frame = 0


Query: 3   VWLCSFGYL-ILLVSVLITTLFINASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLKH 62
           V L SFGYL +LL+ V ITTLFIN      + + SSS+SRR LQKPNKL  +GF V+LKH
Sbjct: 4   VSLRSFGYLQLLLLIVFITTLFIN------TLAFSSSLSRRALQKPNKLPSHGFMVRLKH 63

Query: 63  VDHDGKNLTRLERLRRGVVRGKSRLQRLN------ANGSVGEQVKAPVVAGNGEFLMKLA 122
           VDH  KNLTR ERLRRGV RGK+RL RLN      AN SVG+QVKAPVVAGNGEFLMKLA
Sbjct: 64  VDH-VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLA 123

Query: 123 IGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPT 182
           IG+PPRS  AIMDTGSDLIWTQCKPC+QCFDQATPIFDP QSSSFSKISC   LCGALPT
Sbjct: 124 IGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPT 183

Query: 183 STCTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQG 242
           STC+S GCEYLYTYGDSSSTQGVLA +TFTFGDSTE Q+SI GLGFGCG+DN GDGFSQG
Sbjct: 184 STCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQG 243

Query: 243 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSLLLGSLANINPKTSKDELKTTPLI 302
           AGLVGLGRGPLSLVSQLKEQKFAYCLTAID+SKPSSLLLGSLANI PKTSKDE+K TPLI
Sbjct: 244 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLI 303

Query: 303 RNPSQPSFYYLSLQGISVGDTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLK 362
           +NPSQPSFYYLSLQGISVG TQLSIPKSTFELH+DGSGGVIIDSGTTITYIE+TAF+ LK
Sbjct: 304 KNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLK 363

Query: 363 NEFIAQMSLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRA 422
           NEFIAQM+LPVDDSGTGGLDLCFNLPA  TQVEVPKLTFHFKGADLELPGENYMIGDS+ 
Sbjct: 364 NEFIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKT 423

Query: 423 GLICLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461
           GL+CLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 424 GLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of ClCG04G001160 vs. ExPASy TrEMBL
Match: A0A1S3B573 (aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103486136 PE=3 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.3e-215
Identity = 394/465 (84.73%), Postives = 418/465 (89.89%), Query Frame = 0


Query: 3   VWLCSFGYL-ILLVSVLITTLFINASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLKH 62
           V L SFGYL +LL+ V ITTLFIN      + + SSS+S R LQKPNKL  +GFRV+LKH
Sbjct: 4   VSLRSFGYLQLLLLIVFITTLFIN------TLAFSSSLSTRALQKPNKLPSHGFRVRLKH 63

Query: 63  VDHDGKNLTRLERLRRGVVRGKSRLQRLN------ANGSVGEQVKAPVVAGNGEFLMKLA 122
           VDH  KNLTR ERLRRGV RGK+RL RLN      AN SVG+QVKAPVVAGNGEFLMKLA
Sbjct: 64  VDH-VKNLTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLA 123

Query: 123 IGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPT 182
           IG+PPRS  AIMDTGSDLIWTQCKPC+QCFDQATPIFDP QSSSFSKISC   LCGALPT
Sbjct: 124 IGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPT 183

Query: 183 STCTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQG 242
           STC+S GCEYLYTYGDSSSTQGVLA +TFTFGDSTE Q+SI GLGFGCG+DN GDGFSQG
Sbjct: 184 STCSSDGCEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQG 243

Query: 243 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSLLLGSLANINPKTSKDELKTTPLI 302
           AGLVGLGRGPLSLVSQLKEQKFAYCLTAID+SKPSSLLLGSLANI PKTSKDE+K TPLI
Sbjct: 244 AGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLI 303

Query: 303 RNPSQPSFYYLSLQGISVGDTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLK 362
           +NPSQPSFYYLSLQGISVG TQLSIPKSTFELH+DGSGGVIIDSGTTITYIE+TAF+ LK
Sbjct: 304 KNPSQPSFYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLK 363

Query: 363 NEFIAQMSLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRA 422
           NEFIAQM+LPVDDSGTGGLDLCFNLPA  TQVEVPKLTFHFKGADLELPGENYMIGDS+ 
Sbjct: 364 NEFIAQMNLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKT 423

Query: 423 GLICLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461
           GL+CLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI
Sbjct: 424 GLLCLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

[Close]

BLAST of ClCG04G001160 vs. ExPASy TrEMBL
Match: A0A6J1DKZ2 (aspartic proteinase nepenthesin-1 OS=Momordica charantia OX=3673 GN=LOC111022058 PE=3 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 3.2e-198
Identity = 357/463 (77.11%), Postives = 399/463 (86.18%), Query Frame = 0


Query: 5   LCSFGYLILLVSVLITTLFINASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLKHVDH 64
           LCS  Y I+LV+VL T  FI+       + SSS++SRR LQ+  KL  NGFR++L HVDH
Sbjct: 5   LCSVRYPIVLVAVLATLFFIDL------TVSSSTLSRRALQQ-QKLLNNGFRMRLHHVDH 64

Query: 65  DGKNLTRLERLRRGVVRGKSRLQRLNA-------NGSVGEQVKAPVVAGNGEFLMKLAIG 124
             KNLTR ERL+RG  RG++RLQRLNA         +VG+QV+APVVAGNGEFLMKLAIG
Sbjct: 65  HVKNLTRFERLQRGAARGRNRLQRLNAMVLAAAGGAAVGDQVQAPVVAGNGEFLMKLAIG 124

Query: 125 TPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPTST 184
           +PP+S  AIMDTGSDLIWTQCKPC+QCFDQ+TPIFDP +SSSFSK+SCS  LCGALPTS 
Sbjct: 125 SPPKSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPKESSSFSKLSCSSELCGALPTSA 184

Query: 185 CTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQGAG 244
           C++ GCEYLYTYGD SST G+L  +TFTFGD  E QVSIS +GFGCGDDNEGDGFSQGAG
Sbjct: 185 CSNDGCEYLYTYGDYSSTHGLLGAETFTFGDPGEDQVSISEIGFGCGDDNEGDGFSQGAG 244

Query: 245 LVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSLLLGSLANINPKTSKDELKTTPLIRN 304
           LVGLGRGPLSLVSQLKEQKFAYCLT ID+SKPSSLL+GSLAN+ PK S+DE+KTTPLIRN
Sbjct: 245 LVGLGRGPLSLVSQLKEQKFAYCLTPIDDSKPSSLLMGSLANVKPKKSQDEIKTTPLIRN 304

Query: 305 PSQPSFYYLSLQGISVGDTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNE 364
           PSQPSFYYLSL+GISVG + L+IP+ TFEL +DGSGGVIIDSGTTITYI+ +AFTLLK E
Sbjct: 305 PSQPSFYYLSLEGISVGGSHLAIPQPTFELRDDGSGGVIIDSGTTITYIDKSAFTLLKKE 364

Query: 365 FIAQMSLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGL 424
           FIAQM LPVDDSGTGGLDLCF LP+EATQVEVPKLTFHFK ADLELPGENYMIGDS AGL
Sbjct: 365 FIAQMKLPVDDSGTGGLDLCFKLPSEATQVEVPKLTFHFKDADLELPGENYMIGDSAAGL 424

Query: 425 ICLAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461
           +CLAIGSS GMSIFGNLQQQNFMVVHDLQEET+SF+PTQCD I
Sbjct: 425 LCLAIGSSSGMSIFGNLQQQNFMVVHDLQEETISFVPTQCDGI 460

[Close]

BLAST of ClCG04G001160 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 521.9 bits (1343), Expect = 5.1e-148
Identity = 279/461 (60.52%), Postives = 341/461 (73.97%), Query Frame = 0


Query: 16  SVLITTLFINASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLKHVDHDGKNLTRLERL 75
           S+L     I  S L S SSS  S+  R L  P  L  +GFR+ L+HVD  GKNLT+++++
Sbjct: 8   SLLFPFFLILFSCLISVSSSRRSLIDRTL--PKNLPRSGFRLSLRHVD-SGKNLTKIQKI 67

Query: 76  RRGVVRGKSRLQRLNANGSVG--------EQVKAPVVAGNGEFLMKLAIGTPPRSLWAIM 135
           +RG+ RG  RL RL A   +           +KAP   G+GEFLM+L+IG P     AI+
Sbjct: 68  QRGINRGFHRLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSAIV 127

Query: 136 DTGSDLIWTQCKPCEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPTSTCT--SHGCEY 195
           DTGSDLIWTQCKPC +CFDQ TPIFDP +SSS+SK+ CS  LC ALP S C      CEY
Sbjct: 128 DTGSDLIWTQCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCNALPRSNCNEDKDACEY 187

Query: 196 LYTYGDSSSTQGVLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQGAGLVGLGRGP 255
           LYTYGD SST+G+LA +TFTF D      SISG+GFGCG +NEGDGFSQG+GLVGLGRGP
Sbjct: 188 LYTYGDYSSTRGLLATETFTFEDEN----SISGIGFGCGVENEGDGFSQGSGLVGLGRGP 247

Query: 256 LSLVSQLKEQKFAYCLTAIDESK-PSSLLLGSLAN-INPKTSK----DELKTTPLIRNPS 315
           LSL+SQLKE KF+YCLT+I++S+  SSL +GSLA+ I  KT      +  KT  L+RNP 
Sbjct: 248 LSLISQLKETKFSYCLTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPD 307

Query: 316 QPSFYYLSLQGISVGDTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFI 375
           QPSFYYL LQGI+VG  +LS+ KSTFEL  DG+GG+IIDSGTTITY+E TAF +LK EF 
Sbjct: 308 QPSFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFT 367

Query: 376 AQMSLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLIC 435
           ++MSLPVDDSG+ GLDLCF LP  A  + VPK+ FHFKGADLELPGENYM+ DS  G++C
Sbjct: 368 SRMSLPVDDSGSTGLDLCFKLPDAAKNIAVPKMIFHFKGADLELPGENYMVADSSTGVLC 427

Query: 436 LAIGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461
           LA+GSS GMSIFGN+QQQNF V+HDL++ET+SF+PT+C  +
Sbjct: 428 LAMGSSNGMSIFGNVQQQNFNVLHDLEKETVSFVPTECGKL 461

[Close]

BLAST of ClCG04G001160 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 269.6 bits (688), Expect = 4.6e-72
Identity = 163/425 (38.35%), Postives = 232/425 (54.59%), Query Frame = 0


Query: 56  RVKLKHVDH-DGKNLTRLERLRRGVVRGKSRLQRLN-ANGSVG---------------EQ 115
           RV ++  +H D K+LT L RL R   R KS + RL+ A  ++                + 
Sbjct: 74  RVSVRGTEHSDYKSLT-LARLNRDTARVKSLITRLDLAINNISKADLKPISTMYTTEEQD 133

Query: 116 VKAPVVA----GNGEFLMKLAIGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDP 175
           ++AP+++    G+GE+  ++ IG P R ++ ++DTGSD+ W QC PC  C+ Q  PIF+P
Sbjct: 134 IEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCADCYHQTEPIFEP 193

Query: 176 TQSSSFSKISCSDPLCGALPTSTCTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQV 235
           + SSS+  +SC  P C AL  S C +  C Y  +YGD S T G  A +T T G +    V
Sbjct: 194 SSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDFATETLTIGSTLVQNV 253

Query: 236 SISGLGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSLLL 295
           ++     GCG  NEG  F   AGL+GLG G L+L SQL    F+YCL   D    S++  
Sbjct: 254 AV-----GCGHSNEG-LFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDSDSASTVDF 313

Query: 296 GSLANINPKTSKDELKTTPLIRNPSQPSFYYLSLQGISVGDTQLSIPKSTFELHNDGSGG 355
           G+  +++P     +    PL+RN    +FYYL L GISVG   L IP+S+FE+   GSGG
Sbjct: 314 GT--SLSP-----DAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSGG 373

Query: 356 VIIDSGTTITYIENTAFTLLKNEFIAQMSLPVDDSGTGGLDLCFNLPAEATQVEVPKLTF 415
           +IIDSGT +T ++   +  L++ F+         +G    D C+NL A+ T VEVP + F
Sbjct: 374 IIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTT-VEVPTVAF 433

Query: 416 HFKGAD-LELPGENYMIGDSRAGLICLAIG-SSRGMSIFGNLQQQNFMVVHDLQEETLSF 458
           HF G   L LP +NYMI     G  CLA   ++  ++I GN+QQQ   V  DL    + F
Sbjct: 434 HFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGF 483

[Close]

BLAST of ClCG04G001160 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.7e-71
Identity = 143/368 (38.86%), Postives = 215/368 (58.42%), Query Frame = 0


Query: 97  EQVKAPVVA----GNGEFLMKLAIGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIF 156
           E +  PVV+    G+GE+  ++ +GTP + ++ ++DTGSD+ W QC+PC  C+ Q+ P+F
Sbjct: 145 EDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVF 204

Query: 157 DPTQSSSFSKISCSDPLCGALPTSTCTSHGCEYLYTYGDSSSTQGVLALDTFTFGDSTEL 216
           +PT SS++  ++CS P C  L TS C S+ C Y  +YGD S T G LA DT TFG+S + 
Sbjct: 205 NPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGK- 264

Query: 217 QVSISGLGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDESKPSSL 276
              I+ +  GCG DNEG  F+  AGL+GLG G LS+ +Q+K   F+YCL   D  K SSL
Sbjct: 265 ---INNVALGCGHDNEG-LFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSL 324

Query: 277 LLGSLANINPKTSKDELKTTPLIRNPSQPSFYYLSLQGISVGDTQLSIPKSTFELHNDGS 336
                 + N         T PL+RN    +FYY+ L G SVG  ++ +P + F++   GS
Sbjct: 325 ------DFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGS 384

Query: 337 GGVIIDSGTTITYIENTAFTLLKNEFI-AQMSLPVDDSGTGGLDLCFNLPAEATQVEVPK 396
           GGVI+D GT +T ++  A+  L++ F+   ++L    S     D C++  + +T V+VP 
Sbjct: 385 GGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST-VKVPT 444

Query: 397 LTFHFKGA-DLELPGENYMIGDSRAGLICLAIG-SSRGMSIFGNLQQQNFMVVHDLQEET 456
           + FHF G   L+LP +NY+I    +G  C A   +S  +SI GN+QQQ   + +DL +  
Sbjct: 445 VAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNV 500

Query: 457 LSFLPTQC 458
           +     +C
Sbjct: 505 IGLSGNKC 500

[Close]

BLAST of ClCG04G001160 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-68
Identity = 159/443 (35.89%), Postives = 235/443 (53.05%), Query Frame = 0


Query: 26  ASGLSSSSSSSSSVSRRGLQKPNKLGGNGFRVKLKHVDHDGKNL-----TRLERLRRGVV 85
           AS  SS   S   +S   L   N     GF   L H D           T  +RLR  + 
Sbjct: 2   ASLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIH 61

Query: 86  RGKSRLQRLNANGSVGEQVKAPVVAGNGEFLMKLAIGTPPRSLWAIMDTGSDLIWTQCKP 145
           R  +R+       +   Q +  + + +GE+LM ++IGTPP  + AI DTGSDL+WTQC P
Sbjct: 62  RSVNRVFHFTEKDNT-PQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAP 121

Query: 146 CEQCFDQATPIFDPTQSSSFSKISCSDPLCGALPT-STCTSHG--CEYLYTYGDSSSTQG 205
           C+ C+ Q  P+FDP  SS++  +SCS   C AL   ++C+++   C Y  +YGD+S T+G
Sbjct: 122 CDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKG 181

Query: 206 VLALDTFTFGDSTELQVSISGLGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQ-- 265
            +A+DT T G S    + +  +  GCG +N G    +G+G+VGLG GP+SL+ QL +   
Sbjct: 182 NIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSID 241

Query: 266 -KFAYCLTAIDESKPSSLLLGSLANINPKTSKDELKTTPLIRNPSQPSFYYLSLQGISVG 325
            KF+YCL  +   K  +  +      N   S   + +TPLI   SQ +FYYL+L+ ISVG
Sbjct: 242 GKFSYCLVPLTSKKDQTSKIN--FGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVG 301

Query: 326 DTQLSIPKSTFELHNDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMSLPVDDSGTGGL 385
             Q+    S  E      G +IIDSGTT+T +    ++ L++   + +          GL
Sbjct: 302 SKQIQYSGSDSE---SSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGL 361

Query: 386 DLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNL 445
            LC++   +   ++VP +T HF GAD++L   N  +  S   L+C A   S   SI+GN+
Sbjct: 362 SLCYSATGD---LKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGNV 421

Query: 446 QQQNFMVVHDLQEETLSFLPTQC 458
            Q NF+V +D   +T+SF PT C
Sbjct: 422 AQMNFLVGYDTVSKTVSFKPTDC 434

[Close]

BLAST of ClCG04G001160 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 252.3 bits (643), Expect = 7.5e-67
Identity = 154/432 (35.65%), Postives = 237/432 (54.86%), Query Frame = 0


Query: 44  LQKPNKLGGNGFRVKLKHVDHDGKNL-----TRLERLRRGVVR-GKSRLQRLNANGSVGE 103
           L   N    +GF + L H D           T  +R+R  + R  +S LQ  N + S   
Sbjct: 15  LSNVNAYPKDGFTIDLIHRDSPKSPFYNSAETSSQRMRNAIRRSARSTLQFSNDDASPNS 74

Query: 104 QVKAPVVAGNGEFLMKLAIGTPPRSLWAIMDTGSDLIWTQCKPCEQCFDQATPIFDPTQS 163
             ++ + +  GE+LM ++IGTPP  + AI DTGSDLIWTQC PCE C+ Q +P+FDP +S
Sbjct: 75  P-QSFITSNRGEYLMNISIGTPPVPILAIADTGSDLIWTQCNPCEDCYQQTSPLFDPKES 134

Query: 164 SSFSKISCSDPLCGALPTSTCTS--HGCEYLYTYGDSSSTQGVLALDTFTFGDSTELQVS 223
           S++ K+SCS   C AL  ++C++  + C Y  TYGD+S T+G +A+DT T G S    VS
Sbjct: 135 STYRKVSCSSSQCRALEDASCSTDENTCSYTITYGDNSYTKGDVAVDTVTMGSSGRRPVS 194

Query: 224 ISGLGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQ---KFAYCLTAIDESKPSSL 283
           +  +  GCG +N G     G+G++GLG G  SLVSQL++    KF+YCL       P + 
Sbjct: 195 LRNMIIGCGHENTGTFDPAGSGIIGLGGGSTSLVSQLRKSINGKFSYCLV------PFTS 254

Query: 284 LLGSLANINPKT----SKDELKTTPLIRNPSQPSFYYLSLQGISVGDTQLSIPKSTFELH 343
             G  + IN  T    S D + +T +++     ++Y+L+L+ ISVG  ++    + F   
Sbjct: 255 ETGLTSKINFGTNGIVSGDGVVSTSMVKK-DPATYYFLNLEAISVGSKKIQFTSTIF--- 314

Query: 344 NDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQMSLPVDDSGTGGLDLCFNLPAEATQVE 403
             G G ++IDSGTT+T + +  +  L++   + +         G L LC+    +++  +
Sbjct: 315 GTGEGNIVIDSGTTLTLLPSNFYYELESVVASTIKAERVQDPDGILSLCYR---DSSSFK 374

Query: 404 VPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSRGMSIFGNLQQQNFMVVHDLQEE 461
           VP +T HFKG D++L   N  +  S   + C A  ++  ++IFGNL Q NF+V +D    
Sbjct: 375 VPDITVHFKGGDVKLGNLNTFVAVSE-DVSCFAFAANEQLTIFGNLAQMNFLVGYDTVSG 431

The following BLAST results are available for this feature:

BLAST of ClCG04G001160 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883313.1	2.8e-217	85.38	aspartic proteinase nepenthesin-1 [Benincasa hispida]	[more]
XP_011653928.1	7.0e-216	84.73	aspartic proteinase nepenthesin-1 [Cucumis sativus] >KGN54860.1 hypothetical pro...	[more]
TYK02448.1	2.7e-215	84.73	aspartic proteinase nepenthesin-1 [Cucumis melo var. makuwa]	[more]
XP_008442220.1	2.7e-215	84.73	PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo] >KAA0041170.1 aspart...	[more]
XP_022154910.1	6.6e-198	77.11	aspartic proteinase nepenthesin-1 [Momordica charantia]	[more]

BLAST of ClCG04G001160 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q766C3	3.8e-116	52.83	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	2.3e-108	46.86	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LS40	2.4e-70	38.86	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q6XBF8	2.5e-67	35.89	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q7XV21	5.6e-67	35.20	Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2	[more]

BLAST of ClCG04G001160 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KYT9	3.4e-216	84.73	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G55468...	[more]
A0A5A7TD10	1.3e-215	84.73	Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A5D3BTY9	1.3e-215	84.73	Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3B573	1.3e-215	84.73	aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103486136 PE=3 S...	[more]
A0A6J1DKZ2	3.2e-198	77.11	aspartic proteinase nepenthesin-1 OS=Momordica charantia OX=3673 GN=LOC111022058...	[more]

BLAST of ClCG04G001160 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G03200.1	5.1e-148	60.52	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	4.6e-72	38.35	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	1.7e-71	38.86	Eukaryotic aspartyl protease family protein	[more]
AT5G33340.1	1.8e-68	35.89	Eukaryotic aspartyl protease family protein	[more]
AT1G64830.1	7.5e-67	35.65	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 116..136 score: 42.86 coord: 429..444 score: 26.0 coord: 335..346 score: 38.11
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 110..276 e-value: 2.7E-52 score: 177.6
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 280..460 e-value: 6.2E-53 score: 181.3
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 93..275 e-value: 1.0E-54 score: 187.5
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 104..458
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 303..452 e-value: 2.7E-35 score: 121.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 29..44
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 29..53
None	No IPR available	PANTHER	PTHR47967:SF23	OS08G0469000 PROTEIN	coord: 34..459
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 34..459
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 335..346
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 110..453 score: 42.346478
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 109..457 e-value: 5.55841E-108 score: 318.823

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG04G001160.1	ClCG04G001160.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity