ClCG03G017680 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG03G017680
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionPeptide chain release factor
LocationCG_Chr03: 32794157 .. 32797891 (-)
RNA-Seq ExpressionClCG03G017680
SyntenyClCG03G017680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTGCTTCGTTTAGAGAACCTGCATGGTTTCTCATTCAATTGAAATCGAAAATGTCCATTTTCACGTTCAGAAAACGAGTGTGCAATCGAATCTTCAGCCTCTTCAGAAATGTATTGATCTACAGGCACCGGCTTATCCCAAAACTTCAACGTTCATCAGCTGCTCCCCAAAATTGTGTCCTTTGGAATCATGGGACATCTGATTTTAGCAGGATTTCTGGGTTTCCCTTTAGCACGTTGCGCTTGTTTTCTACACAGGTTGCGGTTGAGCCCTCAACGTCTGATGGGCTTACAGTCGAAGGAATCCTTGGAAATCGATGGACGATTCTGGACGAGAGCGAGAGCGATTGGAGGAGCCATGCTGCTGCAATTGCCCAATCCATTCATCTCATCAAGAAGCGTTTACAGGTTTTAAACATGGTTCCCCATATCTTAGTTTTTCAACTAATTTCTGCTTTACAGCTTTTTCAAGTTTTAGTTTGATGTAAATCAGTTGTGTGCTATCAAAACGCTTGAAGTTTTGATTATCGTTTTTATATTGGCTGTGAATATTGTTTGGCGATGGCAGTGGAAGAAGCTGATGATGAGATTGGAGTTGTTATCGGTGGAGTTGAATAAGGCTGATCTGTGGGATGATCCTGCCCATGCAGGGAAGATAAGTCGGGAGCATGGTTCTATTATGAGTAAGATAAAAGAGATCATGGCATTTGAGAGAGAATTGCTTGAGCATATCGACATGATAAAGCTTGCCAGAGAAGAGAATGATACAGAGTTGGAATCGGTGGGCTCTAAATTCATCTTCTAGGCATGTATTCTTTCTTTTTGTTTTTGTTGTTTGTAAATGTGTTCAGTTTTTTGCATCCCTTCCTCTCAGTATGTGAGGTAAATTATCTAGTTTATGTTATTCCATAAATAAGAACAGAGTAAATAAGAATGCCCTGAAAGAAAGAGATAACTTCGGTGCCCCGTGGTAAGAACTTATGATGGTTGGAGATTGGAGTTCTGGGTTCAACCTTCCATGCTCGAAATAAAGTTTTCCATTTTGGTTTATATTTTGACAGGGATTGCTGTTATTCAATGTTTTAAATGGCAGCCTTGAGGCTTTTCCTCTTTAAGAAGCGAGGTGCAAGCCTCAATTTTTTTTTTTTTTTTAGAACACAACATGCAAATCCTAAATTTGTTTGATTGTTAAACATAATAGTTATATAGTAGTATAAATAGTTCAAATTATTACAATACAACTCAAACCTTTATACTTTAAATAATGGGGTAAATTAATGCTAATCTAAAAATTGTTATATGAGGCGTAAGCCTCACCCCTCAATATGTATTCATAAAGCCGTAAGCCTCCTATGGTTCAAAGAAGGCATACAGTCTTGAAACTAGAAATTAGAGCTTAGCCTCAAGGTGTATCCCTCAATGGTCTTTTAAAACATTTCTGTTTTTATTCCTTTGTAAAATTCTTTCCCATTAACAATTACTTTGGGATATCTGAGGCTGTCATCTCGTCTCAATGAGATAAATGCTAATTTTCTTTTGAAATTAGAACGATTTTTCTAGGGAGTAGATAGATCTTTTTCTTTTGTTTTAATCCTGTTTAGCATGGCCTTTTGCAGGAGTCACTAAGTGCTCTACTCACAATGAGAAGAAATTCAAAAGAGAAAGAGCTTGAAGCTTTATTATCTGGGGAGAACGATTCTTGCTCTTGTTACATAGAGGTCTGCTTAATTTGGTGCTGCTAATCTTTGTTTCTTTTGTGAATTTTCTCGTATGTAGTGTTACTTTTTTTCATTGTCGGATTCAAGTTTTGAGAAATGTTAAGGGTGAATAATATAGAGATACAGCCATCAACCTTTTGAATATTTGTTGATCAAACAGGTTCAAGCTGGAGCTGGCGGAACTGAGAGCATGGACTGGGCAGCGATGGTCATGCAGATGTACAAAATGTGGGCTCAAAGACGCGGTTTCAAGGTTACTGTTATGGAAGAAATGCCGGGTGAGATTGCAGGAATCAAGGTTCAATCTCTGCTAACTTCAAATGTCTATTTCTGAGTATGAGTTCAAATCTGCATGCGCATGTCACTGCTAGCTAATGTAATGTTTAACAGTTGAAAATTCTTATGTCGTTTAACCTTATTAATTGTGTAGCTTGAGTTAAAATTGCCATATCTACTCTATTCAAGCTCTGGCTTGAGTGATAAAATGCCAGTTCTGACAACTTATTCTGTTATTCCAGCGAGCAACAATAAAATTAGATGGTGAATACGCTTTTGGATATGCCAAAGCGGAAGTAGGCGTCCACAGATTGGTTCGCATCTCTCCTTTTGATAGTAACAAGCGGCGACATACTTCGTTTGCTGCTGTAGCTGTCATTCCAATAACTGGTGATGCTTCAACTCATGTTCAAATTAGTGAATCGGATCTTCGAATTGAACGTTTCCGCTCTGGAGGAGCTGGTGGTCAGAGTGTTAACACCACTGATAGTGCCGTGAGGATAGTCCATATACCAACAGGGATTACGGCAACTTGTCAAAATGAAAGGTAAGGAAGTTTAAAACTGGTTCATGTAATGAATAAAACCACTGCTGGATCATTGCTTATTGAAATGGAAAATAACACAGATCACAACATCAAAACAAGGCCTCAGCAATGGCGGTACTTCAGTCTCGATTGAATCAGCTCGAGATGGCACGGCAAGCTCAGATAAATGCACAGCACACACAGAGTCTATCTGATATAACTTGGGGAAACCAAATTCGTAGTTATGTGCTCCATGTGAGTTGTAAACATGGTGTACTTTCAATTCACCTCTTATGTGTTGTTTGTGATTCAGTCATCACTAACTAGTAACTACCAATTGCTTGATTTTTGCAGCCTTATCAAATGGTAAAAGATCTTCGTACCAATTACGAGGTTTCGGATCCTAATTCGGTTCTTGAGGGAGATCTTGACAGCTTTATTTTGAACTATTTGTCGATATCCCTGGACAAAGATGGAGATGAATCGTAAAGTTGTACTATGAGCTATGGTAATTTAGTAACTAATCAATTTTGGCAAACTAGATATTCAGAGCATAATGTATTTAACTAGAAACAGGGGGAAGAACAAAGTGGAGCAGGTTACCTTAGTTCTCTTTAAATTAAAATTTGCCCACTCTTTGAAAATTTCTGGCTCCACCTTGAGTGAAAATAAGCCGCTTTTATTCTATTTTTTTTCAACAGATTTGGAAGTCCCCACAGATTTGAAAGTCCTCTGAATCCATTCATTCAACCAGTTCATCACAGCATCCAATAATGAATAAATCTTATGTTGTTTGTGAAGCAGATGAGCTCTACAAAAGAGAATAGATTGTATTTGCATTTGCATCTTTTTTATGGAAGATGATTCAACATTTGGGCATTTCAAGGTGGAGGGGCTATGGCTATCATATTGAAATGACCAGTTTCATCATCTGAACTCAAGTTAGTGAAGACCTTTTGCGGTTAGGAAAAGCTGCCATGGGCTTTGTTACTCTGTTGTAATAACCATAGCCATTAAAGTATTTATGATTATTCAGACCCAGCCTTCTTTTCTCATAAGAGTTTGTTTTGTTCCTTGGTTCAACCATCTCCCTATTTTGGTTCTTAATTCATTGGCCTATGATGTTATTGAACCTCAATGCGAGGTCGAAGTTGACAATTATGTATGAAAAGTGAAAAGTTTGACAAATGATCTGAACAAATGTATATATGGTG

mRNA sequence

ATTTGCTTCGTTTAGAGAACCTGCATGGTTTCTCATTCAATTGAAATCGAAAATGTCCATTTTCACGTTCAGAAAACGAGTGTGCAATCGAATCTTCAGCCTCTTCAGAAATGTATTGATCTACAGGCACCGGCTTATCCCAAAACTTCAACGTTCATCAGCTGCTCCCCAAAATTGTGTCCTTTGGAATCATGGGACATCTGATTTTAGCAGGATTTCTGGGTTTCCCTTTAGCACGTTGCGCTTGTTTTCTACACAGGTTGCGGTTGAGCCCTCAACGTCTGATGGGCTTACAGTCGAAGGAATCCTTGGAAATCGATGGACGATTCTGGACGAGAGCGAGAGCGATTGGAGGAGCCATGCTGCTGCAATTGCCCAATCCATTCATCTCATCAAGAAGCGTTTACAGTGGAAGAAGCTGATGATGAGATTGGAGTTGTTATCGGTGGAGTTGAATAAGGCTGATCTGTGGGATGATCCTGCCCATGCAGGGAAGATAAGTCGGGAGCATGGTTCTATTATGAGTAAGATAAAAGAGATCATGGCATTTGAGAGAGAATTGCTTGAGCATATCGACATGATAAAGCTTGCCAGAGAAGAGAATGATACAGAGTTGGAATCGGAGTCACTAAGTGCTCTACTCACAATGAGAAGAAATTCAAAAGAGAAAGAGCTTGAAGCTTTATTATCTGGGGAGAACGATTCTTGCTCTTGTTACATAGAGGTTCAAGCTGGAGCTGGCGGAACTGAGAGCATGGACTGGGCAGCGATGGTCATGCAGATGTACAAAATGTGGGCTCAAAGACGCGGTTTCAAGGTTACTGTTATGGAAGAAATGCCGGGTGAGATTGCAGGAATCAAGCGAGCAACAATAAAATTAGATGGTGAATACGCTTTTGGATATGCCAAAGCGGAAGTAGGCGTCCACAGATTGGTTCGCATCTCTCCTTTTGATAGTAACAAGCGGCGACATACTTCGTTTGCTGCTGTAGCTGTCATTCCAATAACTGGTGATGCTTCAACTCATGTTCAAATTAGTGAATCGGATCTTCGAATTGAACGTTTCCGCTCTGGAGGAGCTGGTGGTCAGAGTGTTAACACCACTGATAGTGCCGTGAGGATAGTCCATATACCAACAGGGATTACGGCAACTTGTCAAAATGAAAGATCACAACATCAAAACAAGGCCTCAGCAATGGCGGTACTTCAGTCTCGATTGAATCAGCTCGAGATGGCACGGCAAGCTCAGATAAATGCACAGCACACACAGAGTCTATCTGATATAACTTGGGGAAACCAAATTCGTAGTTATGTGCTCCATCCTTATCAAATGGTAAAAGATCTTCGTACCAATTACGAGGTTTCGGATCCTAATTCGGTTCTTGAGGGAGATCTTGACAGCTTTATTTTGAACTATTTGTCGATATCCCTGGACAAAGATGGAGATGAATCGTAAAGTTGTACTATGAGCTATGATTTGGAAGTCCCCACAGATTTGAAAGTCCTCTGAATCCATTCATTCAACCAGTTCATCACAGCATCCAATAATGAATAAATCTTATGTTGTTTGTGAAGCAGATGAGCTCTACAAAAGAGAATAGATTGTATTTGCATTTGCATCTTTTTTATGGAAGATGATTCAACATTTGGGCATTTCAAGGTGGAGGGGCTATGGCTATCATATTGAAATGACCAGTTTCATCATCTGAACTCAAGTTAGTGAAGACCTTTTGCGGTTAGGAAAAGCTGCCATGGGCTTTGTTACTCTGTTGTAATAACCATAGCCATTAAAGTATTTATGATTATTCAGACCCAGCCTTCTTTTCTCATAAGAGTTTGTTTTGTTCCTTGGTTCAACCATCTCCCTATTTTGGTTCTTAATTCATTGGCCTATGATGTTATTGAACCTCAATGCGAGGTCGAAGTTGACAATTATGTATGAAAAGTGAAAAGTTTGACAAATGATCTGAACAAATGTATATATGGTG

Coding sequence (CDS)

ATGTCCATTTTCACGTTCAGAAAACGAGTGTGCAATCGAATCTTCAGCCTCTTCAGAAATGTATTGATCTACAGGCACCGGCTTATCCCAAAACTTCAACGTTCATCAGCTGCTCCCCAAAATTGTGTCCTTTGGAATCATGGGACATCTGATTTTAGCAGGATTTCTGGGTTTCCCTTTAGCACGTTGCGCTTGTTTTCTACACAGGTTGCGGTTGAGCCCTCAACGTCTGATGGGCTTACAGTCGAAGGAATCCTTGGAAATCGATGGACGATTCTGGACGAGAGCGAGAGCGATTGGAGGAGCCATGCTGCTGCAATTGCCCAATCCATTCATCTCATCAAGAAGCGTTTACAGTGGAAGAAGCTGATGATGAGATTGGAGTTGTTATCGGTGGAGTTGAATAAGGCTGATCTGTGGGATGATCCTGCCCATGCAGGGAAGATAAGTCGGGAGCATGGTTCTATTATGAGTAAGATAAAAGAGATCATGGCATTTGAGAGAGAATTGCTTGAGCATATCGACATGATAAAGCTTGCCAGAGAAGAGAATGATACAGAGTTGGAATCGGAGTCACTAAGTGCTCTACTCACAATGAGAAGAAATTCAAAAGAGAAAGAGCTTGAAGCTTTATTATCTGGGGAGAACGATTCTTGCTCTTGTTACATAGAGGTTCAAGCTGGAGCTGGCGGAACTGAGAGCATGGACTGGGCAGCGATGGTCATGCAGATGTACAAAATGTGGGCTCAAAGACGCGGTTTCAAGGTTACTGTTATGGAAGAAATGCCGGGTGAGATTGCAGGAATCAAGCGAGCAACAATAAAATTAGATGGTGAATACGCTTTTGGATATGCCAAAGCGGAAGTAGGCGTCCACAGATTGGTTCGCATCTCTCCTTTTGATAGTAACAAGCGGCGACATACTTCGTTTGCTGCTGTAGCTGTCATTCCAATAACTGGTGATGCTTCAACTCATGTTCAAATTAGTGAATCGGATCTTCGAATTGAACGTTTCCGCTCTGGAGGAGCTGGTGGTCAGAGTGTTAACACCACTGATAGTGCCGTGAGGATAGTCCATATACCAACAGGGATTACGGCAACTTGTCAAAATGAAAGATCACAACATCAAAACAAGGCCTCAGCAATGGCGGTACTTCAGTCTCGATTGAATCAGCTCGAGATGGCACGGCAAGCTCAGATAAATGCACAGCACACACAGAGTCTATCTGATATAACTTGGGGAAACCAAATTCGTAGTTATGTGCTCCATCCTTATCAAATGGTAAAAGATCTTCGTACCAATTACGAGGTTTCGGATCCTAATTCGGTTCTTGAGGGAGATCTTGACAGCTTTATTTTGAACTATTTGTCGATATCCCTGGACAAAGATGGAGATGAATCGTAA

Protein sequence

MSIFTFRKRVCNRIFSLFRNVLIYRHRLIPKLQRSSAAPQNCVLWNHGTSDFSRISGFPFSTLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHIDMIKLAREENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAMVMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLVRISPFDSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAVRIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGNQIRSYVLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYLSISLDKDGDES
Homology
BLAST of ClCG03G017680 vs. NCBI nr
Match: XP_038878322.1 (peptide chain release factor PrfB2, chloroplastic isoform X1 [Benincasa hispida] >XP_038878323.1 peptide chain release factor PrfB2, chloroplastic isoform X1 [Benincasa hispida] >XP_038878325.1 peptide chain release factor PrfB2, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 852.4 bits (2201), Expect = 1.8e-243
Identity = 439/467 (94.00%), Postives = 450/467 (96.36%), Query Frame = 0

Query: 1   MSIFTFRKRVCNRIFSLFRNVLIYRHRLIPKLQRSSAAPQNCVLWNHGTSDFSRISGFPF 60
           MSIFTF+KRVCNRIFS+F N LI R R IP+LQ SSA  Q+CVLWN G SDF+RISGFPF
Sbjct: 1   MSIFTFKKRVCNRIFSIFTNALISRRRFIPELQCSSATAQSCVLWNRGLSDFNRISGFPF 60

Query: 61  STLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120
           STLRLFST VAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW
Sbjct: 61  STLRLFSTLVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120

Query: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHIDMIKLA 180
           KKLMMRLE LSVELNKADLWDDPAHAGKISREHGSIMSKIKEI AFERELLEHIDMIKLA
Sbjct: 121 KKLMMRLEFLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIKAFERELLEHIDMIKLA 180

Query: 181 REENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240
           REENDTELE ES++ALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM
Sbjct: 181 REENDTELELESINALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240

Query: 241 VMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLVRISPF 300
           VMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLVRISPF
Sbjct: 241 VMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLVRISPF 300

Query: 301 DSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360
           DSNKRRHTSFAAVAVIPITGDAS+HVQI+ESDLRIERFRSGGAGGQSVNTTDSAVRIVHI
Sbjct: 301 DSNKRRHTSFAAVAVIPITGDASSHVQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360

Query: 361 PTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGNQIRSY 420
           PTGITATCQNERSQH NKASAMAVLQSRLNQLEMARQAQ+NAQHTQSLSDITWGNQIRSY
Sbjct: 361 PTGITATCQNERSQHHNKASAMAVLQSRLNQLEMARQAQLNAQHTQSLSDITWGNQIRSY 420

Query: 421 VLHPYQMVKDLRTNYEVSDPNSVLEGD-LDSFILNYLSISLDKDGDE 467
           VLHPYQMVKDLRTNYEVSDP+SVLEGD LDSFILNYLS SLDKDGDE
Sbjct: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLLDSFILNYLSTSLDKDGDE 467

BLAST of ClCG03G017680 vs. NCBI nr
Match: XP_016900916.1 (PREDICTED: peptide chain release factor PrfB2, chloroplastic [Cucumis melo] >XP_016900917.1 PREDICTED: peptide chain release factor PrfB2, chloroplastic [Cucumis melo] >KAA0050946.1 peptide chain release factor PrfB2 [Cucumis melo var. makuwa] >TYK10293.1 peptide chain release factor PrfB2 [Cucumis melo var. makuwa])

HSP 1 Score: 850.9 bits (2197), Expect = 5.2e-243
Identity = 431/466 (92.49%), Postives = 452/466 (97.00%), Query Frame = 0

Query: 1   MSIFTFRKRVCNRIFSLFRNVLIYRHRLIPKLQRSSAAPQNCVLWNHGTSDFSRISGFPF 60
           MSIFTFRK+VCNRIF+LFRN+LI +HR +P+LQ SSA  QN  LWNHG +DF++ISG PF
Sbjct: 1   MSIFTFRKQVCNRIFTLFRNLLISKHRFVPELQYSSATAQNFSLWNHGIADFNKISGLPF 60

Query: 61  STLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120
           +TLRLFSTQ+A+EPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW
Sbjct: 61  TTLRLFSTQIALEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120

Query: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHIDMIKLA 180
           KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEI AFERELLEHIDMIKLA
Sbjct: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180

Query: 181 REENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240
           REENDTELESESL+ALLTMRRNS+EKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM
Sbjct: 181 REENDTELESESLNALLTMRRNSREKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240

Query: 241 VMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLVRISPF 300
           VMQMY MWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAK+EVGVHRLVRISPF
Sbjct: 241 VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300

Query: 301 DSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360
           DSNKRRHTSFAAVAVIPITGD STH+QI+ESDLRIERFRSGGAGGQSVNTTDSAVRIVHI
Sbjct: 301 DSNKRRHTSFAAVAVIPITGDGSTHIQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360

Query: 361 PTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGNQIRSY 420
           PTGITATCQNERSQHQNKA+AMAVLQSRLNQLEM RQAQ+NAQHTQSLSDITWGNQIRSY
Sbjct: 361 PTGITATCQNERSQHQNKAAAMAVLQSRLNQLEMTRQAQMNAQHTQSLSDITWGNQIRSY 420

Query: 421 VLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYLSISLDKDGDE 467
           VLHPYQMVKDLRTNYEVSDP+SVLEGDLDSFILNYLS SLDKDGD+
Sbjct: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSASLDKDGDQ 466

BLAST of ClCG03G017680 vs. NCBI nr
Match: XP_004135582.1 (peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >XP_011659591.1 peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >XP_011659596.1 peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >XP_031738430.1 peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >KGN66015.1 hypothetical protein Csa_007463 [Cucumis sativus])

HSP 1 Score: 840.1 bits (2169), Expect = 9.2e-240
Identity = 428/466 (91.85%), Postives = 446/466 (95.71%), Query Frame = 0

Query: 1   MSIFTFRKRVCNRIFSLFRNVLIYRHRLIPKLQRSSAAPQNCVLWNHGTSDFSRISGFPF 60
           MSIFTFRK+VCNRIF+L RNV I +HR IP LQ SSA  QN  LWN G +DF++I GFPF
Sbjct: 1   MSIFTFRKQVCNRIFTLVRNVFISKHRFIPVLQYSSATAQNFALWNRGITDFNKIFGFPF 60

Query: 61  STLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120
           +TLRLFSTQ+A EPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW
Sbjct: 61  TTLRLFSTQIAFEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120

Query: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHIDMIKLA 180
           KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEI AFERELLEHIDMIKLA
Sbjct: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180

Query: 181 REENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240
           REENDTELESESL+ALLTMRRNS+EKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM
Sbjct: 181 REENDTELESESLNALLTMRRNSREKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240

Query: 241 VMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLVRISPF 300
           VMQMY +WAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAK+EVGVHRLVRISPF
Sbjct: 241 VMQMYILWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300

Query: 301 DSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360
           DSNKRRHTSFAAVAVIPITGD STHVQI+ESDLRIER+RSGGAGGQSVNTTDSAVRIVHI
Sbjct: 301 DSNKRRHTSFAAVAVIPITGDGSTHVQINESDLRIERYRSGGAGGQSVNTTDSAVRIVHI 360

Query: 361 PTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGNQIRSY 420
           PTGITATCQNERSQHQNKA+AMAVLQSRLNQLEM RQAQ+NAQHTQSLSDITWGNQIRSY
Sbjct: 361 PTGITATCQNERSQHQNKAAAMAVLQSRLNQLEMTRQAQMNAQHTQSLSDITWGNQIRSY 420

Query: 421 VLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYLSISLDKDGDE 467
           VLHPYQMVKDLRTNYEVSDP+SVLEGDLDSFILNYLS SLD DGD+
Sbjct: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSASLDNDGDQ 466

BLAST of ClCG03G017680 vs. NCBI nr
Match: XP_022931672.1 (peptide chain release factor PrfB2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 825.9 bits (2132), Expect = 1.8e-235
Identity = 427/468 (91.24%), Postives = 443/468 (94.66%), Query Frame = 0

Query: 1   MSIFTFRKRVCNRIFSLFRN-----VLIYRHRLIPKLQRSSAAPQNCVLWNHGTSDFSRI 60
           MS F   KR+CNR+++LFRN     VLI R RL  +LQ SSA PQN VL+N G SDF  I
Sbjct: 1   MSTFALGKRLCNRVYALFRNSNHRGVLISRRRLFTELQCSSATPQNYVLFNCGVSDFKNI 60

Query: 61  SGFPFSTLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIK 120
           SGFPFSTLRLFSTQVA+EPST+DGLTVEGI+GNRWTIL+ESESDWRSHAAAIAQSIHLIK
Sbjct: 61  SGFPFSTLRLFSTQVAIEPSTADGLTVEGIIGNRWTILEESESDWRSHAAAIAQSIHLIK 120

Query: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHID 180
           KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIM KIKEI AFERELLEHID
Sbjct: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMGKIKEIKAFERELLEHID 180

Query: 181 MIKLAREENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESM 240
           MIKLAREEND ELESESL+ALL+MRRNSKEKELEALL+GENDSCSCYIEVQAGAGGTESM
Sbjct: 181 MIKLAREENDAELESESLNALLSMRRNSKEKELEALLAGENDSCSCYIEVQAGAGGTESM 240

Query: 241 DWAAMVMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV 300
           DWAAMVMQMY MWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV
Sbjct: 241 DWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV 300

Query: 301 RISPFDSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAV 360
           RISPFDSNKRRHTSFAAVAVIPITGDASTHVQI+ESDLRIERFRSGGAGGQSVNTTDSAV
Sbjct: 301 RISPFDSNKRRHTSFAAVAVIPITGDASTHVQINESDLRIERFRSGGAGGQSVNTTDSAV 360

Query: 361 RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGN 420
           RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQ+NAQHTQSLSDITWGN
Sbjct: 361 RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQMNAQHTQSLSDITWGN 420

Query: 421 QIRSYVLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYLSISLDKD 464
           QIRSYVLHPYQMVKDLRTNYEVSDP+SVLEGDLDSFILNYLS SLDKD
Sbjct: 421 QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD 468

BLAST of ClCG03G017680 vs. NCBI nr
Match: XP_023531900.1 (peptide chain release factor PrfB2, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023531901.1 peptide chain release factor PrfB2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 823.5 bits (2126), Expect = 8.9e-235
Identity = 426/468 (91.03%), Postives = 442/468 (94.44%), Query Frame = 0

Query: 1   MSIFTFRKRVCNRIFSLFRN-----VLIYRHRLIPKLQRSSAAPQNCVLWNHGTSDFSRI 60
           MS F   KR+CNR+++LFRN     VLI R RL  +LQ SSA PQN VL+N G SDF  I
Sbjct: 1   MSTFALGKRLCNRLYALFRNSNHGGVLISRRRLFTELQCSSATPQNYVLFNCGVSDFKNI 60

Query: 61  SGFPFSTLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIK 120
           SGFPFS LRLFSTQVA+EPST+DGLTVEGI+GNRWTIL+ESESDWRSHAAAIAQSIHLIK
Sbjct: 61  SGFPFSALRLFSTQVAIEPSTADGLTVEGIIGNRWTILEESESDWRSHAAAIAQSIHLIK 120

Query: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHID 180
           KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIM KIKEI AFERELLEHID
Sbjct: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMGKIKEIKAFERELLEHID 180

Query: 181 MIKLAREENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESM 240
           MIKLAREEND ELESESL+ALL+MRRNSKEKELEALL+GENDSCSCYIEVQAGAGGTESM
Sbjct: 181 MIKLAREENDAELESESLNALLSMRRNSKEKELEALLAGENDSCSCYIEVQAGAGGTESM 240

Query: 241 DWAAMVMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV 300
           DWAAMVMQMY MWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV
Sbjct: 241 DWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV 300

Query: 301 RISPFDSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAV 360
           RISPFDSNKRRHTSFAAVAVIPITGDASTHVQI+ESDLRIERFRSGGAGGQSVNTTDSAV
Sbjct: 301 RISPFDSNKRRHTSFAAVAVIPITGDASTHVQINESDLRIERFRSGGAGGQSVNTTDSAV 360

Query: 361 RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGN 420
           RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQ+NAQHTQSLSDITWGN
Sbjct: 361 RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQMNAQHTQSLSDITWGN 420

Query: 421 QIRSYVLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYLSISLDKD 464
           QIRSYVLHPYQMVKDLRTNYEVSDP+SVLEGDLDSFILNYLS SLDKD
Sbjct: 421 QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD 468

BLAST of ClCG03G017680 vs. ExPASy Swiss-Prot
Match: F4I532 (Peptide chain release factor PrfB2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PRFB2 PE=3 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 3.1e-182
Identity = 326/411 (79.32%), Postives = 366/411 (89.05%), Query Frame = 0

Query: 56  SGFPFSTLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIK 115
           S F  ST  L S  VAV  +T DGLTVE I+ N+W ILDE+E DW+SHAAAIAQSI +IK
Sbjct: 72  SSFASSTRSLSSEAVAV-AATCDGLTVERIIANQWPILDENEGDWKSHAAAIAQSIQVIK 131

Query: 116 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHID 175
           +RLQWKKL++RL++LS ELNK+DLWDDP HAGKISREHGS+  K+K +M FERELLEHID
Sbjct: 132 RRLQWKKLLVRLKVLSAELNKSDLWDDPTHAGKISREHGSLTGKMKGVMTFERELLEHID 191

Query: 176 MIKLAREENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESM 235
           M+KLA+EEND+ELESE+L AL+ MRR SKEKELEALLS +ND CSCYIEVQAGAGGTES 
Sbjct: 192 MLKLAKEENDSELESETLKALIDMRRVSKEKELEALLSADNDPCSCYIEVQAGAGGTESN 251

Query: 236 DWAAMVMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV 295
           DWAAMVM+MYK WAQRR F VTV++E PGEIAGIKRATIK++GEYA+GYAKAEVGVHRLV
Sbjct: 252 DWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIAGIKRATIKVNGEYAYGYAKAEVGVHRLV 311

Query: 296 RISPFDSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAV 355
           RISPFDS KRRHTSFAAVAVIPI GD ST V+I++SDLRIERFRSGGAGGQ  NTTDSAV
Sbjct: 312 RISPFDSGKRRHTSFAAVAVIPILGDGSTRVEINDSDLRIERFRSGGAGGQHANTTDSAV 371

Query: 356 RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGN 415
           RIVHIPTGITATCQNERSQH NKASAMAVLQSRL+QLEMARQ  +NAQHTQSL++I+WGN
Sbjct: 372 RIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLEMARQTAMNAQHTQSLTEISWGN 431

Query: 416 QIRSYVLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYLSISLDKDGDE 467
           QIR+YVLHPY+MVKDLRTNYEVSDP+SVLEGDLD FIL++LS SLDKD  E
Sbjct: 432 QIRTYVLHPYRMVKDLRTNYEVSDPDSVLEGDLDGFILSFLSSSLDKDDPE 481

BLAST of ClCG03G017680 vs. ExPASy Swiss-Prot
Match: Q2W3F8 (Peptide chain release factor 2 OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=prfB PE=3 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 3.4e-88
Identity = 180/371 (48.52%), Postives = 242/371 (65.23%), Query Frame = 0

Query: 101 RSHAAAIAQSIH----LIKKRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSI 160
           R+   A+AQ I     L+K+ L W + +MRL+ L+      DLW+D   A KI RE   +
Sbjct: 2   RAEIEALAQDIRRSAALLKRHLNWDEALMRLDELNASAENPDLWNDAGAAQKIMRERNEL 61

Query: 161 MSKIKEIMAFERELLEHIDMIKLAREENDTELESESLSALLTMRRNSKEKELEALLSGEN 220
            S I+   A EREL +  ++I+L   E D  +  ++   +  ++  + + ELE LLSGE 
Sbjct: 62  DSAIQGCRALERELADLAELIELGEMEGDQTVIDDAEEQVRALKERAAKMELETLLSGEA 121

Query: 221 DSCSCYIEVQAGAGGTESMDWAAMVMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKL 280
           D   CY+E+ AGAGGTES DWA M+++MY  WA++ G+KV  +EE  GE AGIK ATI++
Sbjct: 122 DHNDCYMEINAGAGGTESQDWAEMLLRMYTRWAEKHGYKVEWLEESAGEQAGIKSATIRI 181

Query: 281 DGEYAFGYAKAEVGVHRLVRISPFDSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIE 340
            G  A+G+ K E GVHRLVRISP+DS  RRHTSF++  V P+  D +  +QI+ES+ RI+
Sbjct: 182 LGHNAYGWLKTESGVHRLVRISPYDSAARRHTSFSSAWVYPVIDD-TIDIQINESECRID 241

Query: 341 RFRSGGAGGQSVNTTDSAVRIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEM-A 400
            +R+ GAGGQ +N TDSAVRI HIPTGI   CQ ERSQHQN+A A  +L++RL + E+  
Sbjct: 242 TYRASGAGGQHINKTDSAVRITHIPTGIAVACQMERSQHQNRARAWDMLRARLYEAELQK 301

Query: 401 RQAQINAQHTQSLSDITWGNQIRSYVLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNY 460
           R+A   A   Q  +DI WG+QIRSYVL PYQMVKDLRTN E SD   VL+GDLD F+   
Sbjct: 302 REAAAQALEDQK-TDIGWGHQIRSYVLQPYQMVKDLRTNVETSDTQGVLDGDLDMFMAAS 361

Query: 461 LSISLDKDGDE 467
           L+  +    D+
Sbjct: 362 LAARVQGQVDQ 370

BLAST of ClCG03G017680 vs. ExPASy Swiss-Prot
Match: Q165J6 (Peptide chain release factor 2 OS=Roseobacter denitrificans (strain ATCC 33942 / OCh 114) OX=375451 GN=prfB PE=3 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 6.1e-85
Identity = 175/368 (47.55%), Postives = 248/368 (67.39%), Query Frame = 0

Query: 98  SDWRSHAAAIAQSIHLIKKRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIM 157
           +D ++    I +S+ L+ +RL  +    RLE  +  +    LWDDP  A K+ RE   ++
Sbjct: 3   ADVQNTIEKIQKSLDLLAQRLDVETAPYRLEEFNARVEDPTLWDDPDAAQKLMRERQMLV 62

Query: 158 SKIKEIMAFERELLEHIDMIKLAREENDTELESESLSALLTMRRNSKEKELEALLSGEND 217
             +    + ++E+ ++I++I+L   E+D ++ S++ +AL  +   + +KELEALL GE D
Sbjct: 63  DAMATHDSIKQEMADNIELIELGEMEDDADVVSDAENALRALEETAAKKELEALLDGEAD 122

Query: 218 SCSCYIEVQAGAGGTESMDWAAMVMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLD 277
           S   ++EV AGAGGTES DWA+M+ +MY  WA+++G+KV +     G+ AGIK AT K+ 
Sbjct: 123 SNDTFLEVNAGAGGTESCDWASMLARMYVRWAEKKGYKVELQSMSDGDEAGIKSATYKIT 182

Query: 278 GEYAFGYAKAEVGVHRLVRISPFDSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIER 337
           G  A+G+ K+E GVHRLVRISPFDS  +RHTSF++V V P+  D +  ++++ +D+RI+ 
Sbjct: 183 GLNAYGWLKSESGVHRLVRISPFDSAAKRHTSFSSVWVYPVVDD-NIDIEVNPADIRIDT 242

Query: 338 FRSGGAGGQSVNTTDSAVRIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEM-AR 397
           +RS GAGGQ VNTTDSAVRI H PTGI  T  +E+SQHQN+  AM  L+SRL QLE+  R
Sbjct: 243 YRSSGAGGQHVNTTDSAVRITHHPTGIVVT-SSEKSQHQNRDIAMKALKSRLYQLELDRR 302

Query: 398 QAQINAQHTQSLSDITWGNQIRSYVLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYL 457
            A IN  H ++  D  WGNQIRSYVL PYQMVKDLRTN+E SD   VL+GDLD F+    
Sbjct: 303 NAAINEAH-ENKGDAGWGNQIRSYVLQPYQMVKDLRTNFETSDTKGVLDGDLDGFMA--A 362

Query: 458 SISLDKDG 465
           +++LD  G
Sbjct: 363 TLALDASG 365

BLAST of ClCG03G017680 vs. ExPASy Swiss-Prot
Match: Q1GIB5 (Peptide chain release factor 2 OS=Ruegeria sp. (strain TM1040) OX=292414 GN=prfB PE=3 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.5e-83
Identity = 166/357 (46.50%), Postives = 237/357 (66.39%), Query Frame = 0

Query: 105 AAIAQSIHLIKKRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIM 164
           A I +S+ L+ +R+ W+    RLE  +  +   +LWDDPA A K+ R+  S++  +    
Sbjct: 10  ADIEKSLDLLAQRMNWETAEFRLEEFNARVEDPNLWDDPAAAQKLMRDRQSLVDAMDTYK 69

Query: 165 AFERELLEHIDMIKLAREENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIE 224
           +  ++L + +++I+L   E D E+  ++   L  ++  + +KELEALL GE D    ++E
Sbjct: 70  SIRQDLQDSVELIELGEMEEDAEVVKDAEDTLKALKEKAAQKELEALLDGETDPNDAFLE 129

Query: 225 VQAGAGGTESMDWAAMVMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGY 284
           ++AGAGGTE+ DWA+M+ +MY  WA+ RGF V + EE  G  AGIK AT ++ G  A+G+
Sbjct: 130 IKAGAGGTEACDWASMLARMYVRWAESRGFSVELQEEQAGAEAGIKSATYQIKGHNAYGW 189

Query: 285 AKAEVGVHRLVRISPFDSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAG 344
            K+E G HRLVRISPF     R TSFAAV   P+  D +  ++++ +D+RI+ +RS GAG
Sbjct: 190 LKSESGTHRLVRISPFGKG-TRETSFAAVGAYPVIDD-NIEIEVNPADIRIDTYRSSGAG 249

Query: 345 GQSVNTTDSAVRIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEM-ARQAQINAQ 404
           GQ VNTTDSAVRI H PTGI  T  +E+SQHQN+  AM  L+SRL QLE+  R A++NA 
Sbjct: 250 GQHVNTTDSAVRITHEPTGIVVT-SSEKSQHQNRDIAMKALKSRLYQLELDRRNAEVNAL 309

Query: 405 HTQSLSDITWGNQIRSYVLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYLSISL 461
           H ++  D  WGNQIRSYV+ PYQMVKDLRTN+E +D   VL+GDLD F+   L++ +
Sbjct: 310 H-EAKGDAGWGNQIRSYVMQPYQMVKDLRTNHETADTKGVLDGDLDGFMAATLAMKV 362

BLAST of ClCG03G017680 vs. ExPASy Swiss-Prot
Match: C5CGS4 (Peptide chain release factor 2 OS=Kosmotoga olearia (strain ATCC BAA-1733 / DSM 21960 / TBF 19.5.1) OX=521045 GN=prfB PE=3 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 7.4e-83
Identity = 173/355 (48.73%), Postives = 235/355 (66.20%), Query Frame = 0

Query: 114 IKKRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEH 173
           IK  +   K+  +L  +  EL+   +W D   AGK+ ++  ++ S++ +++   +EL E+
Sbjct: 20  IKTTVDVDKIKEKLSRIEKELSDPSVWSDQRRAGKLGQQAQALRSQL-DLLNKVQELFEN 79

Query: 174 IDMIKLAREENDTELESESLSALLTMRRNS-KEKELEALLSGENDSCSCYIEVQAGAGGT 233
           ID+     EE+++ L  E+L+ LL    +  KE EL  LLS   D+ + Y+ V  GAGGT
Sbjct: 80  IDIAVELSEEDESYL--ENLNELLKEAEDMVKEFELNILLSSPYDAYNAYLSVHPGAGGT 139

Query: 234 ESMDWAAMVMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVH 293
           ES DWA+M+++MY  WA+R  +KVT +EE PGE AGIK ATI + G YA+G  K E GVH
Sbjct: 140 ESQDWASMLLRMYIRWAERNNYKVTTIEEQPGEEAGIKSATINIAGPYAYGKLKYEAGVH 199

Query: 294 RLVRISPFDSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTD 353
           RLVRISPFD+N RRHTSFA+V+V P   D    + I   DL+I+ +R+GGAGGQ VN T+
Sbjct: 200 RLVRISPFDANHRRHTSFASVSVFPEMDD-DVEIDIRPEDLKIDTYRAGGAGGQHVNRTE 259

Query: 354 SAVRIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDIT 413
           SAVRI HIPTGI  TCQNERSQHQNKA+AM +L+++L +LE+ ++ Q   +      DI 
Sbjct: 260 SAVRITHIPTGIVVTCQNERSQHQNKATAMKILKAKLFELELEKKRQEKMKLMGEQKDIA 319

Query: 414 WGNQIRSYVLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFI----LNYLSISLDKD 464
           WGNQIRSYV  PY MVKD RTNYE  D  +V++G +D FI    L + SI  DK+
Sbjct: 320 WGNQIRSYVFQPYTMVKDHRTNYETGDVQAVMDGYIDDFIEKELLFFASIDEDKE 370

BLAST of ClCG03G017680 vs. ExPASy TrEMBL
Match: A0A1S4DY60 (peptide chain release factor PrfB2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492513 PE=3 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 2.5e-243
Identity = 431/466 (92.49%), Postives = 452/466 (97.00%), Query Frame = 0

Query: 1   MSIFTFRKRVCNRIFSLFRNVLIYRHRLIPKLQRSSAAPQNCVLWNHGTSDFSRISGFPF 60
           MSIFTFRK+VCNRIF+LFRN+LI +HR +P+LQ SSA  QN  LWNHG +DF++ISG PF
Sbjct: 1   MSIFTFRKQVCNRIFTLFRNLLISKHRFVPELQYSSATAQNFSLWNHGIADFNKISGLPF 60

Query: 61  STLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120
           +TLRLFSTQ+A+EPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW
Sbjct: 61  TTLRLFSTQIALEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120

Query: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHIDMIKLA 180
           KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEI AFERELLEHIDMIKLA
Sbjct: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180

Query: 181 REENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240
           REENDTELESESL+ALLTMRRNS+EKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM
Sbjct: 181 REENDTELESESLNALLTMRRNSREKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240

Query: 241 VMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLVRISPF 300
           VMQMY MWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAK+EVGVHRLVRISPF
Sbjct: 241 VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300

Query: 301 DSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360
           DSNKRRHTSFAAVAVIPITGD STH+QI+ESDLRIERFRSGGAGGQSVNTTDSAVRIVHI
Sbjct: 301 DSNKRRHTSFAAVAVIPITGDGSTHIQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360

Query: 361 PTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGNQIRSY 420
           PTGITATCQNERSQHQNKA+AMAVLQSRLNQLEM RQAQ+NAQHTQSLSDITWGNQIRSY
Sbjct: 361 PTGITATCQNERSQHQNKAAAMAVLQSRLNQLEMTRQAQMNAQHTQSLSDITWGNQIRSY 420

Query: 421 VLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYLSISLDKDGDE 467
           VLHPYQMVKDLRTNYEVSDP+SVLEGDLDSFILNYLS SLDKDGD+
Sbjct: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSASLDKDGDQ 466

BLAST of ClCG03G017680 vs. ExPASy TrEMBL
Match: A0A5D3CEK4 (Peptide chain release factor PrfB2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004440 PE=3 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 2.5e-243
Identity = 431/466 (92.49%), Postives = 452/466 (97.00%), Query Frame = 0

Query: 1   MSIFTFRKRVCNRIFSLFRNVLIYRHRLIPKLQRSSAAPQNCVLWNHGTSDFSRISGFPF 60
           MSIFTFRK+VCNRIF+LFRN+LI +HR +P+LQ SSA  QN  LWNHG +DF++ISG PF
Sbjct: 1   MSIFTFRKQVCNRIFTLFRNLLISKHRFVPELQYSSATAQNFSLWNHGIADFNKISGLPF 60

Query: 61  STLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120
           +TLRLFSTQ+A+EPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW
Sbjct: 61  TTLRLFSTQIALEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120

Query: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHIDMIKLA 180
           KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEI AFERELLEHIDMIKLA
Sbjct: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180

Query: 181 REENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240
           REENDTELESESL+ALLTMRRNS+EKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM
Sbjct: 181 REENDTELESESLNALLTMRRNSREKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240

Query: 241 VMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLVRISPF 300
           VMQMY MWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAK+EVGVHRLVRISPF
Sbjct: 241 VMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300

Query: 301 DSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360
           DSNKRRHTSFAAVAVIPITGD STH+QI+ESDLRIERFRSGGAGGQSVNTTDSAVRIVHI
Sbjct: 301 DSNKRRHTSFAAVAVIPITGDGSTHIQINESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360

Query: 361 PTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGNQIRSY 420
           PTGITATCQNERSQHQNKA+AMAVLQSRLNQLEM RQAQ+NAQHTQSLSDITWGNQIRSY
Sbjct: 361 PTGITATCQNERSQHQNKAAAMAVLQSRLNQLEMTRQAQMNAQHTQSLSDITWGNQIRSY 420

Query: 421 VLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYLSISLDKDGDE 467
           VLHPYQMVKDLRTNYEVSDP+SVLEGDLDSFILNYLS SLDKDGD+
Sbjct: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSASLDKDGDQ 466

BLAST of ClCG03G017680 vs. ExPASy TrEMBL
Match: A0A0A0LVX3 (RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G561950 PE=3 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 4.5e-240
Identity = 428/466 (91.85%), Postives = 446/466 (95.71%), Query Frame = 0

Query: 1   MSIFTFRKRVCNRIFSLFRNVLIYRHRLIPKLQRSSAAPQNCVLWNHGTSDFSRISGFPF 60
           MSIFTFRK+VCNRIF+L RNV I +HR IP LQ SSA  QN  LWN G +DF++I GFPF
Sbjct: 1   MSIFTFRKQVCNRIFTLVRNVFISKHRFIPVLQYSSATAQNFALWNRGITDFNKIFGFPF 60

Query: 61  STLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120
           +TLRLFSTQ+A EPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW
Sbjct: 61  TTLRLFSTQIAFEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQW 120

Query: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHIDMIKLA 180
           KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEI AFERELLEHIDMIKLA
Sbjct: 121 KKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEINAFERELLEHIDMIKLA 180

Query: 181 REENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240
           REENDTELESESL+ALLTMRRNS+EKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM
Sbjct: 181 REENDTELESESLNALLTMRRNSREKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAM 240

Query: 241 VMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLVRISPF 300
           VMQMY +WAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAK+EVGVHRLVRISPF
Sbjct: 241 VMQMYILWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKSEVGVHRLVRISPF 300

Query: 301 DSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAVRIVHI 360
           DSNKRRHTSFAAVAVIPITGD STHVQI+ESDLRIER+RSGGAGGQSVNTTDSAVRIVHI
Sbjct: 301 DSNKRRHTSFAAVAVIPITGDGSTHVQINESDLRIERYRSGGAGGQSVNTTDSAVRIVHI 360

Query: 361 PTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGNQIRSY 420
           PTGITATCQNERSQHQNKA+AMAVLQSRLNQLEM RQAQ+NAQHTQSLSDITWGNQIRSY
Sbjct: 361 PTGITATCQNERSQHQNKAAAMAVLQSRLNQLEMTRQAQMNAQHTQSLSDITWGNQIRSY 420

Query: 421 VLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYLSISLDKDGDE 467
           VLHPYQMVKDLRTNYEVSDP+SVLEGDLDSFILNYLS SLD DGD+
Sbjct: 421 VLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSASLDNDGDQ 466

BLAST of ClCG03G017680 vs. ExPASy TrEMBL
Match: A0A6J1EUC0 (peptide chain release factor PrfB2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111437810 PE=3 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 8.7e-236
Identity = 427/468 (91.24%), Postives = 443/468 (94.66%), Query Frame = 0

Query: 1   MSIFTFRKRVCNRIFSLFRN-----VLIYRHRLIPKLQRSSAAPQNCVLWNHGTSDFSRI 60
           MS F   KR+CNR+++LFRN     VLI R RL  +LQ SSA PQN VL+N G SDF  I
Sbjct: 1   MSTFALGKRLCNRVYALFRNSNHRGVLISRRRLFTELQCSSATPQNYVLFNCGVSDFKNI 60

Query: 61  SGFPFSTLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIK 120
           SGFPFSTLRLFSTQVA+EPST+DGLTVEGI+GNRWTIL+ESESDWRSHAAAIAQSIHLIK
Sbjct: 61  SGFPFSTLRLFSTQVAIEPSTADGLTVEGIIGNRWTILEESESDWRSHAAAIAQSIHLIK 120

Query: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHID 180
           KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIM KIKEI AFERELLEHID
Sbjct: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMGKIKEIKAFERELLEHID 180

Query: 181 MIKLAREENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESM 240
           MIKLAREEND ELESESL+ALL+MRRNSKEKELEALL+GENDSCSCYIEVQAGAGGTESM
Sbjct: 181 MIKLAREENDAELESESLNALLSMRRNSKEKELEALLAGENDSCSCYIEVQAGAGGTESM 240

Query: 241 DWAAMVMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV 300
           DWAAMVMQMY MWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV
Sbjct: 241 DWAAMVMQMYIMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV 300

Query: 301 RISPFDSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAV 360
           RISPFDSNKRRHTSFAAVAVIPITGDASTHVQI+ESDLRIERFRSGGAGGQSVNTTDSAV
Sbjct: 301 RISPFDSNKRRHTSFAAVAVIPITGDASTHVQINESDLRIERFRSGGAGGQSVNTTDSAV 360

Query: 361 RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGN 420
           RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQ+NAQHTQSLSDITWGN
Sbjct: 361 RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQMNAQHTQSLSDITWGN 420

Query: 421 QIRSYVLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYLSISLDKD 464
           QIRSYVLHPYQMVKDLRTNYEVSDP+SVLEGDLDSFILNYLS SLDKD
Sbjct: 421 QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD 468

BLAST of ClCG03G017680 vs. ExPASy TrEMBL
Match: A0A6J1JP75 (peptide chain release factor PrfB2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111486461 PE=3 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 6.2e-234
Identity = 423/468 (90.38%), Postives = 442/468 (94.44%), Query Frame = 0

Query: 1   MSIFTFRKRVCNRIFSLFRN-----VLIYRHRLIPKLQRSSAAPQNCVLWNHGTSDFSRI 60
           MS F   KR+CNR+++LFRN     VLI R RL  +LQ SSA PQN VL+N G SDF  I
Sbjct: 1   MSSFALGKRLCNRVYALFRNSNHRGVLISRRRLFTELQCSSATPQNYVLFNCGVSDFKNI 60

Query: 61  SGFPFSTLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIK 120
           SGFPFSTLRLFSTQVA+EPST+DGLTVEGI+GNRWTIL+ESESDWRSHAAAIAQSIHLIK
Sbjct: 61  SGFPFSTLRLFSTQVAIEPSTADGLTVEGIIGNRWTILEESESDWRSHAAAIAQSIHLIK 120

Query: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHID 180
           KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIM KIKE+ AFERELLEHID
Sbjct: 121 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMGKIKEMKAFERELLEHID 180

Query: 181 MIKLAREENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESM 240
           MIKLAREEND ELESESL+ALL+MRRNSKEKELEALL+GENDSCSCYIEVQAGAGGTESM
Sbjct: 181 MIKLAREENDAELESESLNALLSMRRNSKEKELEALLAGENDSCSCYIEVQAGAGGTESM 240

Query: 241 DWAAMVMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV 300
           DWAAMVMQMY MWAQR GFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV
Sbjct: 241 DWAAMVMQMYIMWAQRHGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV 300

Query: 301 RISPFDSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAV 360
           RISPFDSNKRRHTSFAAVAVIPI+GDASTHVQI+E+DLRIERFRSGGAGGQSVNTTDSAV
Sbjct: 301 RISPFDSNKRRHTSFAAVAVIPISGDASTHVQINEADLRIERFRSGGAGGQSVNTTDSAV 360

Query: 361 RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGN 420
           RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQ+NAQHTQSLSDITWGN
Sbjct: 361 RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQMNAQHTQSLSDITWGN 420

Query: 421 QIRSYVLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYLSISLDKD 464
           QIRSYVLHPYQMVKDLRTNYEVSDP+SVLEGDLDSFILNYLS SLDKD
Sbjct: 421 QIRSYVLHPYQMVKDLRTNYEVSDPDSVLEGDLDSFILNYLSTSLDKD 468

BLAST of ClCG03G017680 vs. TAIR 10
Match: AT1G56350.1 (Peptide chain release factor 2 )

HSP 1 Score: 639.4 bits (1648), Expect = 2.2e-183
Identity = 326/411 (79.32%), Postives = 366/411 (89.05%), Query Frame = 0

Query: 56  SGFPFSTLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIK 115
           S F  ST  L S  VAV  +T DGLTVE I+ N+W ILDE+E DW+SHAAAIAQSI +IK
Sbjct: 72  SSFASSTRSLSSEAVAV-AATCDGLTVERIIANQWPILDENEGDWKSHAAAIAQSIQVIK 131

Query: 116 KRLQWKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHID 175
           +RLQWKKL++RL++LS ELNK+DLWDDP HAGKISREHGS+  K+K +M FERELLEHID
Sbjct: 132 RRLQWKKLLVRLKVLSAELNKSDLWDDPTHAGKISREHGSLTGKMKGVMTFERELLEHID 191

Query: 176 MIKLAREENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESM 235
           M+KLA+EEND+ELESE+L AL+ MRR SKEKELEALLS +ND CSCYIEVQAGAGGTES 
Sbjct: 192 MLKLAKEENDSELESETLKALIDMRRVSKEKELEALLSADNDPCSCYIEVQAGAGGTESN 251

Query: 236 DWAAMVMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLV 295
           DWAAMVM+MYK WAQRR F VTV++E PGEIAGIKRATIK++GEYA+GYAKAEVGVHRLV
Sbjct: 252 DWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIAGIKRATIKVNGEYAYGYAKAEVGVHRLV 311

Query: 296 RISPFDSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAV 355
           RISPFDS KRRHTSFAAVAVIPI GD ST V+I++SDLRIERFRSGGAGGQ  NTTDSAV
Sbjct: 312 RISPFDSGKRRHTSFAAVAVIPILGDGSTRVEINDSDLRIERFRSGGAGGQHANTTDSAV 371

Query: 356 RIVHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGN 415
           RIVHIPTGITATCQNERSQH NKASAMAVLQSRL+QLEMARQ  +NAQHTQSL++I+WGN
Sbjct: 372 RIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLEMARQTAMNAQHTQSLTEISWGN 431

Query: 416 QIRSYVLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYLSISLDKDGDE 467
           QIR+YVLHPY+MVKDLRTNYEVSDP+SVLEGDLD FIL++LS SLDKD  E
Sbjct: 432 QIRTYVLHPYRMVKDLRTNYEVSDPDSVLEGDLDGFILSFLSSSLDKDDPE 481

BLAST of ClCG03G017680 vs. TAIR 10
Match: AT5G36170.1 (high chlorophyll fluorescent 109 )

HSP 1 Score: 234.2 bits (596), Expect = 2.2e-61
Identity = 135/399 (33.83%), Postives = 219/399 (54.89%), Query Frame = 0

Query: 60  FSTLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQ 119
           F++  +F+ Q +    +++  T E ++ + +T+        R      +  +  I+    
Sbjct: 52  FNSPIVFAAQESNLSVSNENETSEWLMQDFYTL--------RKDVEIASARVEEIRASAN 111

Query: 120 WKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHIDMIKL 179
            ++L   +  L  +      WDD   A +       +  +++ +  F+  + +   ++KL
Sbjct: 112 LQQLEQEITNLESKATDTSFWDDRTKAQETLSSLNDLKDRMRLLSEFKTMVEDAETIVKL 171

Query: 180 ARE--ENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDW 239
             E    D  L  E++  +  + ++  + EL  LLSG  D     + + AGAGGT++ DW
Sbjct: 172 TEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTDAQDW 231

Query: 240 AAMVMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLVRI 299
           A M+++MY  W +++ +K  V+E   GE AGIK AT++++G YA+GY   E G HR+VR 
Sbjct: 232 ADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHRIVRQ 291

Query: 300 SPFDSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAVRI 359
           SPF+S   R TSF+ V V+P+  + +  ++I E DL I   R+GG GGQ+VN  ++AVRI
Sbjct: 292 SPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRI 351

Query: 360 VHIPTGITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGNQI 419
            HIPTG+   C  ERSQ  NK  A+  L+++L  +   ++A    +         WG QI
Sbjct: 352 THIPTGVAVRCTEERSQLANKTRALIRLKAKLMVIAEEQRATEIKEIRGDAVKAEWGQQI 411

Query: 420 RSYVLHPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYL 457
           R+YV HPY++VKD+RT +E SD  SV++GDLD FI  YL
Sbjct: 412 RNYVFHPYKLVKDVRTGHETSDITSVMDGDLDPFIKAYL 442

BLAST of ClCG03G017680 vs. TAIR 10
Match: AT5G36170.2 (high chlorophyll fluorescent 109 )

HSP 1 Score: 233.8 bits (595), Expect = 2.8e-61
Identity = 134/394 (34.01%), Postives = 216/394 (54.82%), Query Frame = 0

Query: 65  LFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQWKKLM 124
           +F+ Q +    +++  T E ++ + +T+        R      +  +  I+     ++L 
Sbjct: 56  IFAAQESNLSVSNENETSEWLMQDFYTL--------RKDVEIASARVEEIRASANLQQLE 115

Query: 125 MRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHIDMIKLARE-- 184
             +  L  +      WDD   A +       +  +++ +  F+  + +   ++KL  E  
Sbjct: 116 QEITNLESKATDTSFWDDRTKAQETLSSLNDLKDRMRLLSEFKTMVEDAETIVKLTEEMD 175

Query: 185 ENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDWAAMVM 244
             D  L  E++  +  + ++  + EL  LLSG  D     + + AGAGGT++ DWA M++
Sbjct: 176 STDVSLLEEAMGIIKELNKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTDAQDWADMLL 235

Query: 245 QMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLVRISPFDS 304
           +MY  W +++ +K  V+E   GE AGIK AT++++G YA+GY   E G HR+VR SPF+S
Sbjct: 236 RMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHRIVRQSPFNS 295

Query: 305 NKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAVRIVHIPT 364
              R TSF+ V V+P+  + +  ++I E DL I   R+GG GGQ+VN  ++AVRI HIPT
Sbjct: 296 KGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIPT 355

Query: 365 GITATCQNERSQHQNKASAMAVLQSRLNQLEMARQAQINAQHTQSLSDITWGNQIRSYVL 424
           G+   C  ERSQ  NK  A+  L+++L  +   ++A    +         WG QIR+YV 
Sbjct: 356 GVAVRCTEERSQLANKTRALIRLKAKLMVIAEEQRATEIKEIRGDAVKAEWGQQIRNYVF 415

Query: 425 HPYQMVKDLRTNYEVSDPNSVLEGDLDSFILNYL 457
           HPY++VKD+RT +E SD  SV++GDLD FI  YL
Sbjct: 416 HPYKLVKDVRTGHETSDITSVMDGDLDPFIKAYL 441

BLAST of ClCG03G017680 vs. TAIR 10
Match: AT5G36170.3 (high chlorophyll fluorescent 109 )

HSP 1 Score: 169.5 bits (428), Expect = 6.5e-42
Identity = 97/311 (31.19%), Postives = 166/311 (53.38%), Query Frame = 0

Query: 60  FSTLRLFSTQVAVEPSTSDGLTVEGILGNRWTILDESESDWRSHAAAIAQSIHLIKKRLQ 119
           F++  +F+ Q +    +++  T E ++ + +T+        R      +  +  I+    
Sbjct: 52  FNSPIVFAAQESNLSVSNENETSEWLMQDFYTL--------RKDVEIASARVEEIRASAN 111

Query: 120 WKKLMMRLELLSVELNKADLWDDPAHAGKISREHGSIMSKIKEIMAFERELLEHIDMIKL 179
            ++L   +  L  +      WDD   A +       +  +++ +  F+  + +   ++KL
Sbjct: 112 LQQLEQEITNLESKATDTSFWDDRTKAQETLSSLNDLKDRMRLLSEFKTMVEDAETIVKL 171

Query: 180 ARE--ENDTELESESLSALLTMRRNSKEKELEALLSGENDSCSCYIEVQAGAGGTESMDW 239
             E    D  L  E++  +  + ++  + EL  LLSG  D     + + AGAGGT++ DW
Sbjct: 172 TEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTDAQDW 231

Query: 240 AAMVMQMYKMWAQRRGFKVTVMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLVRI 299
           A M+++MY  W +++ +K  V+E   GE AGIK AT++++G YA+GY   E G HR+VR 
Sbjct: 232 ADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHRIVRQ 291

Query: 300 SPFDSNKRRHTSFAAVAVIPITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAVRI 359
           SPF+S   R TSF+ V V+P+  + +  ++I E DL I   R+GG GGQ+VN  ++AVRI
Sbjct: 292 SPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRI 351

Query: 360 VHIPTGITATC 369
            HIPTG+   C
Sbjct: 352 THIPTGVAVRC 354

BLAST of ClCG03G017680 vs. TAIR 10
Match: AT2G47020.1 (Peptide chain release factor 1 )

HSP 1 Score: 149.8 bits (377), Expect = 5.3e-36
Identity = 105/317 (33.12%), Postives = 171/317 (53.94%), Query Frame = 0

Query: 144 AHAGKISREHGSIMSKIKEIMAFERELLEHIDMIKLAREENDTELESESLSALLTMRRNS 203
           + A K  R+    M  I ++ A ++E    ID +K    E+  + +   L+         
Sbjct: 85  SRANKELRKLRDSMLLINDLRAKQKE----IDGLKSLVSESSDDKDMLDLAVGELDEAVE 144

Query: 204 KEKELEALL------SGENDSCSCYIEVQAGAGGTESMDWAAMVMQMYKMWAQRRGFKVT 263
           +EK L+ LL        E D   C +EV+AG GG E+  +A  + +MY+ ++Q++G+K  
Sbjct: 145 EEKRLQTLLLKSLLPKDEADERDCILEVRAGTGGEEASLFAMDIFRMYERYSQKKGWKFD 204

Query: 264 VMEEMPGEIAGIKRATIKLDGEYAFGYAKAEVGVHRLVRISPFDSNKRRHTSFAAVAVIP 323
           +++    ++ G K A+  + G   +G  K E G+HR+ RI   + + R HTS  +VA++P
Sbjct: 205 IVDITESDMKGYKEASAAICGASVYGKLKFESGIHRVQRIPITEKSGRIHTSAISVAILP 264

Query: 324 ITGDASTHVQISESDLRIERFRSGGAGGQSVNTTDSAVRIVHIPTGITATCQNERSQHQN 383
              +    VQ+   DLRI+ +RSGG+GGQ  NTT+SAVRI+H+PTG+  + Q+ERSQH N
Sbjct: 265 QADEVD--VQLRNEDLRIDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSIQDERSQHMN 324

Query: 384 KASAMAVLQSRLNQLEMAR-QAQINAQHTQSLSDITWGNQIRSYVLHPYQMVKDLRTNYE 443
           +A A+ VL +RL ++E  R Q+  +   +  +       +IR+Y   P   V D R    
Sbjct: 325 RAKALKVLCARLYEIERLRIQSSRSKLRSDQIGSGDRSGRIRTYNF-PQGRVTDHRVGIT 384

Query: 444 VSDPNSVLEGD-LDSFI 453
                 +++G+ LD FI
Sbjct: 385 HHAIEDMMQGENLDMFI 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878322.11.8e-24394.00peptide chain release factor PrfB2, chloroplastic isoform X1 [Benincasa hispida]... [more]
XP_016900916.15.2e-24392.49PREDICTED: peptide chain release factor PrfB2, chloroplastic [Cucumis melo] >XP_... [more]
XP_004135582.19.2e-24091.85peptide chain release factor PrfB2, chloroplastic [Cucumis sativus] >XP_01165959... [more]
XP_022931672.11.8e-23591.24peptide chain release factor PrfB2, chloroplastic [Cucurbita moschata][more]
XP_023531900.18.9e-23591.03peptide chain release factor PrfB2, chloroplastic [Cucurbita pepo subsp. pepo] >... [more]
Match NameE-valueIdentityDescription
F4I5323.1e-18279.32Peptide chain release factor PrfB2, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q2W3F83.4e-8848.52Peptide chain release factor 2 OS=Magnetospirillum magneticum (strain AMB-1 / AT... [more]
Q165J66.1e-8547.55Peptide chain release factor 2 OS=Roseobacter denitrificans (strain ATCC 33942 /... [more]
Q1GIB51.5e-8346.50Peptide chain release factor 2 OS=Ruegeria sp. (strain TM1040) OX=292414 GN=prfB... [more]
C5CGS47.4e-8348.73Peptide chain release factor 2 OS=Kosmotoga olearia (strain ATCC BAA-1733 / DSM ... [more]
Match NameE-valueIdentityDescription
A0A1S4DY602.5e-24392.49peptide chain release factor PrfB2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A5D3CEK42.5e-24392.49Peptide chain release factor PrfB2 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A0A0LVX34.5e-24091.85RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G561950 P... [more]
A0A6J1EUC08.7e-23691.24peptide chain release factor PrfB2, chloroplastic OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1JP756.2e-23490.38peptide chain release factor PrfB2, chloroplastic OS=Cucurbita maxima OX=3661 GN... [more]
Match NameE-valueIdentityDescription
AT1G56350.12.2e-18379.32Peptide chain release factor 2 [more]
AT5G36170.12.2e-6133.83high chlorophyll fluorescent 109 [more]
AT5G36170.22.8e-6134.01high chlorophyll fluorescent 109 [more]
AT5G36170.36.5e-4231.19high chlorophyll fluorescent 109 [more]
AT2G47020.15.3e-3633.12Peptide chain release factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005139Peptide chain release factorSMARTSM00937PCRF_a_2coord: 178..288
e-value: 2.3E-41
score: 153.4
IPR005139Peptide chain release factorPFAMPF03462PCRFcoord: 126..313
e-value: 3.7E-51
score: 173.7
NoneNo IPR availableGENE3D3.30.70.1660coord: 223..452
e-value: 2.2E-91
score: 306.8
NoneNo IPR availableGENE3D1.20.58.410Release factorcoord: 98..209
e-value: 4.6E-12
score: 47.8
NoneNo IPR availableGENE3D3.30.160.20coord: 319..415
e-value: 2.2E-91
score: 306.8
NoneNo IPR availablePANTHERPTHR43116PEPTIDE CHAIN RELEASE FACTOR 2coord: 93..465
NoneNo IPR availablePANTHERPTHR43116:SF3PEPTIDE CHAIN RELEASE FACTOR PRFB1, CHLOROPLASTIC-RELATEDcoord: 93..465
NoneNo IPR availableSUPERFAMILY75620Release factorcoord: 124..459
IPR004374Peptide chain release factor 2TIGRFAMTIGR00020TIGR00020coord: 99..457
e-value: 2.4E-130
score: 432.7
IPR004374Peptide chain release factor 2HAMAPMF_00094Rel_fac_2coord: 96..460
score: 35.714111
IPR000352Peptide chain release factor class IPFAMPF00472RF-1coord: 325..432
e-value: 7.1E-36
score: 122.7
IPR000352Peptide chain release factor class IPROSITEPS00745RF_PROK_Icoord: 339..355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG03G017680.1ClCG03G017680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006415 translational termination
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016149 translation release factor activity, codon specific
molecular_function GO:0003747 translation release factor activity