ClCG03G016540 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG03G016540
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Descriptionvesicle-associated protein 2-2-like isoform X2
LocationCG_Chr03: 31692475 .. 31697429 (-)
RNA-Seq ExpressionClCG03G016540
SyntenyClCG03G016540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTCTTTTTCTTTTTCTTTTTCAATCACAATATTCCACAACTTTGTGGTATTCAATGGACTGTGTTTCTTACTCCTATGGCGTTCGAAATGATGACCCAATTCATCTACTAGTCCCCCACTTTCTTCTTTTCTTCCAACCTTTTGAAGCTCATCGGTTGAGTTCTGGTGACTGGAAAAATTCATTATGTGATCGCTATTACAATACTGTAATAAACATTTTCGGAAATTTCTTGTTGATTCTAATTTGTGAGTTTTTTTATTTTTTATTATTTATTTATACCTTAAACTCTGTAGAGATGTATTTCGAATATTATTATATTAGTGATTGTTTATTGTAGAAATCGTAGGGGTGTTTTTATAATCGATGGGTTCGAATAATTTAATTCGGTTCCTTGTTTGTATTTGGATGCATGTCTTCTGTTGTTTTGTTTATTCTGAGCTTCTATGGTTATGTTCGGCTTGTGAGTTTTCTTTTAATACACTTAATCCCCAAAAGTCTTTGCTTCAATTTTTCATGTTTTTTTTTTGTATTTCGATTGTGCAGGACAATTGTTTGATTAAATAGCCGAGTGAATTGCTTTTCAATCTAAGTTAAGTATGGATACGAGGCTTTTGGAAATTCATCCTCGGGAATTGAAGTTCATTTGTAAGTCTACATAATGAATTATTTTCTCTCTTTAGACTTTTGATTCGTTGTTCTCAATGGGCTGTTTACCGATTACCTCTGGAAAGATCAGGAAATTAGCTGCTGCTCTTGTTTCTTCCTATGAACCGTTTGATCTTTTGTGGCAAAAGCAAAGTGATGTTCTTGTTTGGGTGTTCATTTAGTGCTTTTTAGTTTTTGTAATTTATGTTTTAGTTCTTTCATTTGTACTTTGTTTTGTATGCTAATTGATCACAGCCGAAGGGTAAAAAAGTGCCTTTGCATAACTAAAAACATTTTTCAAATTTTGGTCGGTATTTGGTTGTGTATAGATAGGACAACATGGTTTTTATTTGAAAAACACTTAAAAGGTACTATTTGGAGAAGCGCTTAGATTTAGGTTTGAAATGTGTTTTGGACCTAATGATTTTAGGTGAAACACATTTTTATAAGCACGTTTGATAAACGAACTAATTAAGTGCTTACTTTTGAAGTATAATAAACAAACTTTTCAGTATTTAAAAAAATTTTAAAAGCACTTTTGACTTAATCAGTCGCCGGCAGTTTATAAAGCATTTTTTATAGTTAAAAGTACTTTTTACTCTTCTAAAAACGATATTAAACTCACGTTTTGTGCTTTTCCTGAAAGTGCTTATTAAAAATAGTGTTTTTTGAAACTTAAAAGTTATCTTAAAGCTGAAGTTTTGATGTATGATATTTTCTATGATGTCTGCTCTGGATTTAATTTCCTAAGCTACCTCAAACGAATTGTTGTATCTGCTTTTCTGAATATATTTTCTCATTTGCTACTAGGACATTGCTTACTTTAGACTTATTTATTATTATTTTTGTATGAACAAGGATATGTTATTTTCTACTTCATTTTATCATTCACTCCCTTTTCCAAATTAGAACTAATATCGTTCATATTTCCTTCTATGTCCTCCAGTTGAACTGAATAAACATAGCTTGTGCTCAATTCAACTAACAAATAATACGCTCGACCATGTTGCTTTTAAGGTATGGGTTACCAATCTTCCTCTCTGTCTCTGTCTCATTTTTTCTCTCTCTTGTACTTTTCAAAAAGCAAAAATCAATTGAACTGATTTATGATTCATCATGTAGAGATAGCATGTTATGTTGCATGCAAGATAATGGACAGTTTATTTTTTCAGGTTAAGATCACATCCCCCAAGAAGTACTATGTCCGGCCAAAGGTTGGAATTATCGAGCCAAACTCAACTCGTGAATTCACGGGTATTTACTTTTGACTAGTTAAGGCTTTACATTTTCTCTCTTCTATGGGTGGTATATTTATTTCTAGATGTTGATAAATTGAAGTCATGAGTGATCTTTTATCTTAAATAGCTTTAATGAGGCCTCATTGTGGCTACATTCTTCTGCTTCTAGCATCTTTGCTTGATTAGCTTTATCTTTATTTTTTCTATGCTTTCATTTCATGCCGTAGTCTATTTGGTGTACTTGCATCTTATACAGAAACTGTAAACCTATTTTTTTTTTTCAACTTAAGGGTACAGCCTGTCTTGTTTGACCTGTGTAGATTTATAAGATCTTTATGTTGGTCAGTAAGGGGAAATCACACTCTACAAGTTGGTTATGTAATGCAGAATAGGGAAGGAAAGGAAGAGGGGCCCACTAAGGAATGTGGCCTGTATATACGCCCGGGAGTGAGGGATGTTTCTTCCTGTGCTTTTTGTGAATTTGATGTTGCAAGAGAGGAGGGATGGAGAGATTACTCTTCCCTCTCTAATCGGCTGGGAGATTTTGTATCGCCTCAAGGCTTAACTTTCCTCTGTTTATCAATATATAGTAAGGGAGATACCTTACACAATGATTGGCTCAAGTGCTTGCACTAATCATGGGCTATTCTCTGTTTTGTCATATTGCTCTCATTCTTTCATTATCCCTTTCTCTCTTTCTTTTCATGTCCTGCACTTGCCTATAGCTTTTCCTGCTTTCCAAATTTTATATAATGGTTCTTAATGTAGTTAGGATGCAAGCACAAAAGCTGGCCCCACCTGATATGTTATGCAAAGACAAGTTCTTAATCCAGAGCACAGTAGTTCCTGCTGATACAACTGATGAGGATATAACTTCTAGCTTGGTAAATGGTTCTACTACTATTTATTATGTATAATTGGCTATTTGTTTTGGAATTTAACGTTTTTCTTCTCATAAATTTCAGTTTAGCAAAGAAGGTGGGAAGTACATAGAAGAACAGAAGTTGGGTGTAACCCTTGTTAGCCCACCCATTTCCCAAGTGTTTTCCCCAGTTGATGAAGCAAAAAGGGAGGAGGAGGTGTTTCTTGGAGTTACAGAACCAAATAATCTATGTAAAGCAGTAGAGGAGATTGTAGAACAGGAACCTTTAAACAAAAACCATGTAATGTCTAATGGAGTTGATAGCCTGCCGCTAAAATACATGATTACAATTTCTCAATACATGCCTTTTTTGTATGCTTGCTTCAAAATACATTTACTTTTATCCCAAGTAGGATACTTAGATTAGCAATCATGGTAGAGTAGTTTTGAGAACGCCCTGGAAGCTTATAACATGGAAGCATCGAGACTTGGCACATGACTATAGCTAAATGTTTTGTTAAATTTTTTATGAAATGAAATGCATCTCTCTTCCAATCCAGCTATAACCATTTTTCTTTGCTTATTGAAATACAAAATTTTGGTTGTCCTGTTTATTGTCCCAGGTTCCCAGCAATGCTATGGAGCCTGAACCGGCAAGTTTAGCTAACGACTTCGTAGATGGCAACGGGGAGGACCTAGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAGCTCAGTGAACTTGAGTTAAAGCTCTGTCAGGTCAGTCTTCCATAAAAATTGCTCCCATATTATGAACTCTGAGCATAATGATGAAGTGGAAAGATTTTCCAGAAACCTTATTTAAACCGTTAACTGAAGAAATTCTGCTAAACAGTGATGTGTATTGAAAAACTTTCTTTACAGGCTCAAAATGCCATTTCAAAGCTAAAGGAGGAGAAGCAAATAAGCATCCAAGAAACCAAATTGTTGCAGGAGAATTTTGTAATTTCCTGTACCATTTTATTCTTTTTTCTTAAACGTATAATTCATATAACAATTAAAAGACCAATGCCATTTATTGTTGCCTATGTTATAACATCAAAGTACATCTGAAGACAGTTTATAGTTTCTGTTGGTCTGTTGAAGTCCAAATATAGTTTTTCCTGTCAACATCCTTAGATACCTGCATGAACTTTCCTCCAGTTTTCTTCAACTGAAATGAAGTCAGGGGTTACAAATTTAGCGAATGAACTTTGATAGTGATATCTAGTTGGTCCCTAAACCTTAAAAAGTTTTAATTACCCCTCTAAGACTTTGAATCATGATTTTATATAGTTAGTGTTTACACTGTTGATAAATAATAATGCAACATTGGTGGATAAATGTCGGCTGCTTTAGCAGAATATGGTAGACAAAAGATGAAAGGAGTGGATAATAGGAGAAAATAAGTGTCATAGTCTATTAAAGGGATTTGATGTTACATTGCTTCAACTAGATAGTACACTAAGCATATTACTTTATCTTCTCTAGTTGTGCACTTTTGTCTCAGTCATATTAATATTGAAACATTTAAAATTTAAAAGACTGAATTGAAGATTGAACTATGAAATACCATGGTAAAAAATCTGTATGTTTCGAGGACGGTTTGTTTCAGATTCTTGCTAAATTTGGACATCTGCTTAGCAATATGATTACATATTCTAGTTTTATAGTTGCAAATTTGTTGTACTATGATTTGTTTTGAATCTCAAATTCCTGAAGGCTGTGCATTTTGTAAATTTGCATTTTGCAGTCAGAGTTGAGGAAGACAGAGCTCAATAGCGTTCAAGTTGGCTTTCCTGTTCTGTATCTCTACATGGTGGCACTTGTATGTACTTTTCTAGGACGCTTCTTGAGGTAGTAAAGATTTCTAGTGTCATTATATGTGTGTATCTTTTGCATATTGTCAAAGGAGGGGTTGGGATTGGTGTATAGCTTTGAAGTAAGAATAACTGTATAGGTTCTATTCTCCCTTATGACTCCCTCTCGGTTCGGATACCGCTTTCGTTGTGAATTTGAGCATAACTTAAGTAGTTCAATATTAGTGTCACTCCCAACTTGAGTATAGCACATTTGACAAGTTATCGACGTCTTCATAAGTCTAAATTGAGACACATCTTAAATGTGTTATAGTGTTAATGTTAGTATTACTATTTTAATGTAATTATCTTTGTCATAGTCGTT

mRNA sequence

TTTTCTTTTTCTTTTTCTTTTTCAATCACAATATTCCACAACTTTGTGGTATTCAATGGACTGTGTTTCTTACTCCTATGGCGTTCGAAATGATGACCCAATTCATCTACTAGTCCCCCACTTTCTTCTTTTCTTCCAACCTTTTGAAGCTCATCGGTTGAGTTCTGGTGACTGGAAAAATTCATTATGTGATCGCTATTACAATACTGTAATAAACATTTTCGGAAATTTCTTGTTGATTCTAATTTGACAATTGTTTGATTAAATAGCCGAGTGAATTGCTTTTCAATCTAAGTTAAGTATGGATACGAGGCTTTTGGAAATTCATCCTCGGGAATTGAAGTTCATTTTTGAACTGAATAAACATAGCTTGTGCTCAATTCAACTAACAAATAATACGCTCGACCATGTTGCTTTTAAGGTTAAGATCACATCCCCCAAGAAGTACTATGTCCGGCCAAAGGTTGGAATTATCGAGCCAAACTCAACTCGTGAATTCACGGTTAGGATGCAAGCACAAAAGCTGGCCCCACCTGATATGTTATGCAAAGACAAGTTCTTAATCCAGAGCACAGTAGTTCCTGCTGATACAACTGATGAGGATATAACTTCTAGCTTGTTTAGCAAAGAAGGTGGGAAGTACATAGAAGAACAGAAGTTGGGTGTAACCCTTGTTAGCCCACCCATTTCCCAAGTGTTTTCCCCAGTTGATGAAGCAAAAAGGGAGGAGGAGGTGTTTCTTGGAGTTACAGAACCAAATAATCTATGTAAAGCAGTAGAGGAGATTGTAGAACAGGAACCTTTAAACAAAAACCATGTTCCCAGCAATGCTATGGAGCCTGAACCGGCAAGTTTAGCTAACGACTTCGTAGATGGCAACGGGGAGGACCTAGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAGCTCAGTGAACTTGAGTTAAAGCTCTGTCAGGCTCAAAATGCCATTTCAAAGCTAAAGGAGGAGAAGCAAATAAGCATCCAAGAAACCAAATTGTTGCAGGAGAATTTTTCAGAGTTGAGGAAGACAGAGCTCAATAGCGTTCAAGTTGGCTTTCCTGTTCTGTATCTCTACATGGTGGCACTTGTATGTACTTTTCTAGGACGCTTCTTGAGGTAGTAAAGATTTCTAGTGTCATTATATGTGTGTATCTTTTGCATATTGTCAAAGGAGGGGTTGGGATTGGTGTATAGCTTTGAAGTAAGAATAACTGTATAGGTTCTATTCTCCCTTATGACTCCCTCTCGGTTCGGATACCGCTTTCGTTGTGAATTTGAGCATAACTTAAGTAGTTCAATATTAGTGTCACTCCCAACTTGAGTATAGCACATTTGACAAGTTATCGACGTCTTCATAAGTCTAAATTGAGACACATCTTAAATGTGTTATAGTGTTAATGTTAGTATTACTATTTTAATGTAATTATCTTTGTCATAGTCGTT

Coding sequence (CDS)

ATGGATACGAGGCTTTTGGAAATTCATCCTCGGGAATTGAAGTTCATTTTTGAACTGAATAAACATAGCTTGTGCTCAATTCAACTAACAAATAATACGCTCGACCATGTTGCTTTTAAGGTTAAGATCACATCCCCCAAGAAGTACTATGTCCGGCCAAAGGTTGGAATTATCGAGCCAAACTCAACTCGTGAATTCACGGTTAGGATGCAAGCACAAAAGCTGGCCCCACCTGATATGTTATGCAAAGACAAGTTCTTAATCCAGAGCACAGTAGTTCCTGCTGATACAACTGATGAGGATATAACTTCTAGCTTGTTTAGCAAAGAAGGTGGGAAGTACATAGAAGAACAGAAGTTGGGTGTAACCCTTGTTAGCCCACCCATTTCCCAAGTGTTTTCCCCAGTTGATGAAGCAAAAAGGGAGGAGGAGGTGTTTCTTGGAGTTACAGAACCAAATAATCTATGTAAAGCAGTAGAGGAGATTGTAGAACAGGAACCTTTAAACAAAAACCATGTTCCCAGCAATGCTATGGAGCCTGAACCGGCAAGTTTAGCTAACGACTTCGTAGATGGCAACGGGGAGGACCTAGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAGCTCAGTGAACTTGAGTTAAAGCTCTGTCAGGCTCAAAATGCCATTTCAAAGCTAAAGGAGGAGAAGCAAATAAGCATCCAAGAAACCAAATTGTTGCAGGAGAATTTTTCAGAGTTGAGGAAGACAGAGCTCAATAGCGTTCAAGTTGGCTTTCCTGTTCTGTATCTCTACATGGTGGCACTTGTATGTACTTTTCTAGGACGCTTCTTGAGGTAG

Protein sequence

MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEPNSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLGVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAMEPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR
Homology
BLAST of ClCG03G016540 vs. NCBI nr
Match: XP_038878362.1 (vesicle-associated protein 1-3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 478.4 bits (1230), Expect = 4.2e-131
Identity = 251/288 (87.15%), Postives = 261/288 (90.62%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           MD RLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKY+VRPKVGII P
Sbjct: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           NST EFTVRMQAQ LAPPDMLCKDKFLIQSTVVPADTTD DITS+LF+KEGGKYIEEQKL
Sbjct: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAM-- 180
           GVTLVSP IS V SPVDEA +EEEV L VTEPNNLCKAVEEIV+QEPLNKNHVP NA   
Sbjct: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATEP 180

Query: 181 ------EPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240
                 EPEP+ L NDF+DGN E+LEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ
Sbjct: 181 RDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240

Query: 241 ISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           ISIQETK L+ENFSELRKTELN VQVGFPVLYLYMVA+VCTFLGR LR
Sbjct: 241 ISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRLLR 288

BLAST of ClCG03G016540 vs. NCBI nr
Match: XP_011659931.1 (vesicle-associated protein 2-1 [Cucumis sativus] >XP_011659932.1 vesicle-associated protein 2-1 [Cucumis sativus] >XP_031740596.1 vesicle-associated protein 2-1 [Cucumis sativus] >XP_031740621.1 vesicle-associated protein 2-1 [Cucumis sativus] >KGN66132.1 hypothetical protein Csa_007310 [Cucumis sativus])

HSP 1 Score: 440.7 bits (1132), Expect = 9.7e-120
Identity = 236/288 (81.94%), Postives = 249/288 (86.46%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M+TRLLEIHPRELKFIFELNK SLCSIQLTN T+DHVAFKVKITSPKKY+VRPKVGIIEP
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           NSTREFTVRMQ Q+LAPPDMLCKDKFLIQSTVVPADTTD DI SSLF+KEGGKYIEEQKL
Sbjct: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIVSSLFAKEGGKYIEEQKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSN---- 180
           GV LV+P  S VFSP DEA            PNNLC+AVEEIV+QEPLNKNHVP N    
Sbjct: 121 GVILVTPS-SPVFSPADEA------------PNNLCEAVEEIVDQEPLNKNHVPDNNTEP 180

Query: 181 ----AMEPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240
                 EPEPAS ANDF+D N +++EMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ
Sbjct: 181 RDVVEKEPEPASSANDFIDSNEDNVEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240

Query: 241 ISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           ISIQETK LQENFSELRKTELN V+VGFPVLYLYMVALVCTFLGR LR
Sbjct: 241 ISIQETKFLQENFSELRKTELNRVKVGFPVLYLYMVALVCTFLGRLLR 275

BLAST of ClCG03G016540 vs. NCBI nr
Match: XP_008450729.1 (PREDICTED: vesicle-associated protein 1-3-like [Cucumis melo])

HSP 1 Score: 439.5 bits (1129), Expect = 2.2e-119
Identity = 235/288 (81.60%), Postives = 247/288 (85.76%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M+TRLLEIHPRELKFIFELNK SLCSIQLTN T+DHVAFKVKITSPKKY+VRPKVGIIEP
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           NSTR FTVRMQ Q+LAPPDMLCKDKFLIQSTVVPADTTD DI SSLF KEGGKYIEEQKL
Sbjct: 61  NSTRGFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFVKEGGKYIEEQKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSN---- 180
           GV LV+P IS VF+PVDEA             NNLCKAVEEIV+QEPLNKNHVP N    
Sbjct: 121 GVILVTPSISPVFTPVDEAS------------NNLCKAVEEIVDQEPLNKNHVPDNKTEP 180

Query: 181 ----AMEPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240
                 EPEPASLAN+F+D N ++ EMIKHLEELKLKLSELELKLCQAQN+ISKLKEEKQ
Sbjct: 181 RDVVEKEPEPASLANNFIDSNEDNAEMIKHLEELKLKLSELELKLCQAQNSISKLKEEKQ 240

Query: 241 ISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           ISIQETK LQENFSELRK ELN VQVGFPVLYLYMVALVCTFLGR LR
Sbjct: 241 ISIQETKFLQENFSELRKKELNRVQVGFPVLYLYMVALVCTFLGRLLR 276

BLAST of ClCG03G016540 vs. NCBI nr
Match: KAG7023965.1 (Vesicle-associated protein 2-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 421.8 bits (1083), Expect = 4.7e-114
Identity = 235/302 (77.81%), Postives = 249/302 (82.45%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           MD RLLEIHP+ELKFI E NKHSLCSIQLTNN LDHVAFKVK TSP KY+VRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           NSTREFTVRMQAQKLA PDMLCKD+FLIQSTVVPADTTDEDITSSLF+K+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAM-- 180
           GVTLVSP ISQ       A +EEE  LGVT PNNL  AVEEIV+QEP +++HV SN +  
Sbjct: 121 GVTLVSPSISQ-------ALKEEEA-LGVTGPNNLSTAVEEIVDQEPSHESHVVSNGVDS 180

Query: 181 --------------------EPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLC 240
                               EPEPASLAND VDG+ E+LEMIK LEELKLKLSELELKLC
Sbjct: 181 LPPKHMVPGNGTDPRDVGEEEPEPASLANDVVDGDLENLEMIKDLEELKLKLSELELKLC 240

Query: 241 QAQNAISKLKEEKQISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRF 281
           +AQNAISKLKEEKQISIQE K LQENFSELRKTEL  VQVGFPVLYLYMVALVCTFLGRF
Sbjct: 241 EAQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRF 294

BLAST of ClCG03G016540 vs. NCBI nr
Match: XP_022961473.1 (vesicle-associated protein 2-1-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 421.0 bits (1081), Expect = 8.0e-114
Identity = 233/302 (77.15%), Postives = 249/302 (82.45%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           MD RLLEIHP+ELKFI E NKHSLCSIQLTNN LDHVAFKVK TSP KY+VRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           NSTREFTVRMQAQKLA PDMLCKD+FLIQSTVVPADTTDEDITSSLF+K+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAM-- 180
           GVTL+SP ISQ       A +EEE  LGVT PNNL  AVEEIV+QEP +++HV SN +  
Sbjct: 121 GVTLISPSISQ-------ALKEEEA-LGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDS 180

Query: 181 --------------------EPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLC 240
                               EPEPASLAND +DG+ E+LEMIK LEELKLKLSELELKLC
Sbjct: 181 LPPKHMVPGNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLC 240

Query: 241 QAQNAISKLKEEKQISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRF 281
           +AQNAISKLKEEKQISIQE K LQENFSELRKTEL  VQVGFPVLYLYMVALVCTFLGRF
Sbjct: 241 EAQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRF 294

BLAST of ClCG03G016540 vs. ExPASy Swiss-Prot
Match: B9DHD7 (Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 6.7e-47
Identity = 143/385 (37.14%), Postives = 187/385 (48.57%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M+  LL+I PR L+F  +L K + C +QLTN T  +VAFKVK TSPKKY VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
            ST EFTV MQA K  PPDM+CKDKFLIQST V A+TTDEDIT+S+FSK  GK+IEE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKR----EEEVFLG------------------VTEPN----- 180
            VTLV P  S   SP++  K+    E+ +                     V EP      
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 ----------------------------NLCKAVEEIVEQE------------------- 240
                                       N  KA ++  +                     
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 --------------------PLNKNHVPSNAMEPEP-ASLANDFV-----DGNG----ED 281
                               P ++      A++ EP  S   D V     DG      ++
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

BLAST of ClCG03G016540 vs. ExPASy Swiss-Prot
Match: Q8VZ95 (Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 5.9e-35
Identity = 100/279 (35.84%), Postives = 140/279 (50.18%), Query Frame = 0

Query: 2   DTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEPN 61
           ++ LL + P +L+F FEL K   CS+ LTN T ++VAFKVK T+PKKY VRP  G++ P 
Sbjct: 19  NSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPR 78

Query: 62  STREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLG 121
           ST E  V MQAQK AP DM CKDKFL+Q  +     T +++T  +FSKE G  +EE KL 
Sbjct: 79  STCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLR 138

Query: 122 VTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAMEPE 181
           VT V+PP  +  SPV E   E                                       
Sbjct: 139 VTYVAPP--RPPSPVHEGSEEGS------------------------------------S 198

Query: 182 PASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKLL 241
           P +  +D  +G+G +    + + + K    E      +A+  I+KL EEKQ +IQ    L
Sbjct: 199 PRASVSD--NGHGSEFSFERFIVDNKAGHQE---NTSEARALITKLTEEKQSAIQLNNRL 254

Query: 242 QENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           Q    +LR+    S   G P +Y+ +V L+   LG  ++
Sbjct: 259 QRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIMK 254

BLAST of ClCG03G016540 vs. ExPASy Swiss-Prot
Match: Q9SHC8 (Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 5.5e-33
Identity = 96/280 (34.29%), Postives = 134/280 (47.86%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M   LL I P +L+F FEL K   CS+ L N T ++VAFKVK T+PKKY VRP  G++ P
Sbjct: 1   MSNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
            S+ E  V MQAQK AP D+ CKDKFL+Q  V     T +D+T  +FSKE G  +EE KL
Sbjct: 61  RSSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAMEP 180
            V  V+PP  +  SPV E   E                                      
Sbjct: 121 RVVYVAPP--RPPSPVREGSEEGS------------------------------------ 180

Query: 181 EPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKL 240
            P +  +D  +GN  D          ++   +      +A+  ++KL EEK  ++Q    
Sbjct: 181 SPRASVSD--NGNASDFTAAPRFSADRVDAQD---NSSEARALVTKLTEEKNSAVQLNNR 237

Query: 241 LQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           LQ+   +LR+    S   G P +Y+ +V L+   LG  ++
Sbjct: 241 LQQELDQLRRESKRSKSGGIPFMYVLLVGLIGLILGYIMK 237

BLAST of ClCG03G016540 vs. ExPASy Swiss-Prot
Match: Q84WW5 (Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.8e-31
Identity = 105/278 (37.77%), Postives = 142/278 (51.08%), Query Frame = 0

Query: 5   LLEIHPRELKFIFELNKHSLCSIQLTNNTLDH-VAFKVKITSPKKYYVRPKVGIIEPNST 64
           L+ IHP ELKF FEL K S CS+QLTN T    VAFKVK T+P+KY VRP  G++ P  +
Sbjct: 6   LVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDS 65

Query: 65  REFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLGVT 124
              TV MQAQK AP DM CKDKFL+Q+ VV   TT +++ + +F+KE G+ IE+ KL V 
Sbjct: 66  CNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVV 125

Query: 125 LV--SPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAMEPE 184
            +  +PP     SPV E         G  E N+   ++ +I  Q                
Sbjct: 126 YIPANPP-----SPVPE---------GSEEGNSPMASLNDIASQS--------------- 185

Query: 185 PASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKLL 244
            ASL +D          + +  EE   K SE       A + ISKL EEK  + Q+++ L
Sbjct: 186 -ASLFDD----------VSRTFEETSEKSSE-------AWSMISKLTEEKTSATQQSQKL 236

Query: 245 QENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFL 280
           +     LRK        G  +L + +V L+   +G  L
Sbjct: 246 RLELEMLRKETSKKQSGGHSLLLMLLVGLLGCVIGYLL 236

BLAST of ClCG03G016540 vs. ExPASy Swiss-Prot
Match: Q9LVU1 (Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.0e-30
Identity = 63/130 (48.46%), Postives = 90/130 (69.23%), Query Frame = 0

Query: 2   DTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEPN 61
           + +L+ I P ELKF+FEL K S C +++ N T ++VAFKVK TSPKKY+VRP  G+I+P 
Sbjct: 6   ENQLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPW 65

Query: 62  STREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLG 121
            +    V +QAQ+  PPDM CKDKFL+QST+VP  T  +++    F+K+ GK + E KL 
Sbjct: 66  DSCIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLK 125

Query: 122 VTLVSPPISQ 132
           V+ ++P  +Q
Sbjct: 126 VSYITPSTTQ 135

BLAST of ClCG03G016540 vs. ExPASy TrEMBL
Match: A0A0A0LZ53 (MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G573020 PE=4 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 4.7e-120
Identity = 236/288 (81.94%), Postives = 249/288 (86.46%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M+TRLLEIHPRELKFIFELNK SLCSIQLTN T+DHVAFKVKITSPKKY+VRPKVGIIEP
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           NSTREFTVRMQ Q+LAPPDMLCKDKFLIQSTVVPADTTD DI SSLF+KEGGKYIEEQKL
Sbjct: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIVSSLFAKEGGKYIEEQKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSN---- 180
           GV LV+P  S VFSP DEA            PNNLC+AVEEIV+QEPLNKNHVP N    
Sbjct: 121 GVILVTPS-SPVFSPADEA------------PNNLCEAVEEIVDQEPLNKNHVPDNNTEP 180

Query: 181 ----AMEPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240
                 EPEPAS ANDF+D N +++EMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ
Sbjct: 181 RDVVEKEPEPASSANDFIDSNEDNVEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240

Query: 241 ISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           ISIQETK LQENFSELRKTELN V+VGFPVLYLYMVALVCTFLGR LR
Sbjct: 241 ISIQETKFLQENFSELRKTELNRVKVGFPVLYLYMVALVCTFLGRLLR 275

BLAST of ClCG03G016540 vs. ExPASy TrEMBL
Match: A0A1S3BPW9 (vesicle-associated protein 1-3-like OS=Cucumis melo OX=3656 GN=LOC103492221 PE=4 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.1e-119
Identity = 235/288 (81.60%), Postives = 247/288 (85.76%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M+TRLLEIHPRELKFIFELNK SLCSIQLTN T+DHVAFKVKITSPKKY+VRPKVGIIEP
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           NSTR FTVRMQ Q+LAPPDMLCKDKFLIQSTVVPADTTD DI SSLF KEGGKYIEEQKL
Sbjct: 61  NSTRGFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFVKEGGKYIEEQKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSN---- 180
           GV LV+P IS VF+PVDEA             NNLCKAVEEIV+QEPLNKNHVP N    
Sbjct: 121 GVILVTPSISPVFTPVDEAS------------NNLCKAVEEIVDQEPLNKNHVPDNKTEP 180

Query: 181 ----AMEPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240
                 EPEPASLAN+F+D N ++ EMIKHLEELKLKLSELELKLCQAQN+ISKLKEEKQ
Sbjct: 181 RDVVEKEPEPASLANNFIDSNEDNAEMIKHLEELKLKLSELELKLCQAQNSISKLKEEKQ 240

Query: 241 ISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           ISIQETK LQENFSELRK ELN VQVGFPVLYLYMVALVCTFLGR LR
Sbjct: 241 ISIQETKFLQENFSELRKKELNRVQVGFPVLYLYMVALVCTFLGRLLR 276

BLAST of ClCG03G016540 vs. ExPASy TrEMBL
Match: A0A6J1HAA2 (vesicle-associated protein 2-1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462041 PE=4 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 3.9e-114
Identity = 233/302 (77.15%), Postives = 249/302 (82.45%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           MD RLLEIHP+ELKFI E NKHSLCSIQLTNN LDHVAFKVK TSP KY+VRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           NSTREFTVRMQAQKLA PDMLCKD+FLIQSTVVPADTTDEDITSSLF+K+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAM-- 180
           GVTL+SP ISQ       A +EEE  LGVT PNNL  AVEEIV+QEP +++HV SN +  
Sbjct: 121 GVTLISPSISQ-------ALKEEEA-LGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDS 180

Query: 181 --------------------EPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLC 240
                               EPEPASLAND +DG+ E+LEMIK LEELKLKLSELELKLC
Sbjct: 181 LPPKHMVPGNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLC 240

Query: 241 QAQNAISKLKEEKQISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRF 281
           +AQNAISKLKEEKQISIQE K LQENFSELRKTEL  VQVGFPVLYLYMVALVCTFLGRF
Sbjct: 241 EAQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRF 294

BLAST of ClCG03G016540 vs. ExPASy TrEMBL
Match: A0A6J1HBX8 (vesicle-associated protein 2-1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462041 PE=4 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 3.9e-114
Identity = 233/302 (77.15%), Postives = 249/302 (82.45%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           MD RLLEIHP+ELKFI E NKHSLCSIQLTNN LDHVAFKVK TSP KY+VRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           NSTREFTVRMQAQKLA PDMLCKD+FLIQSTVVPADTTDEDITSSLF+K+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAM-- 180
           GVTL+SP ISQ       A +EEE  LGVT PNNL  AVEEIV+QEP +++HV SN +  
Sbjct: 121 GVTLISPSISQ-------ALKEEEA-LGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDS 180

Query: 181 --------------------EPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLC 240
                               EPEPASLAND +DG+ E+LEMIK LEELKLKLSELELKLC
Sbjct: 181 LPPKHMVPGNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLC 240

Query: 241 QAQNAISKLKEEKQISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRF 281
           +AQNAISKLKEEKQISIQE K LQENFSELRKTEL  VQVGFPVLYLYMVALVCTFLGRF
Sbjct: 241 EAQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRF 294

BLAST of ClCG03G016540 vs. ExPASy TrEMBL
Match: A0A6J1I0M1 (vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467905 PE=4 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 1.2e-112
Identity = 231/302 (76.49%), Postives = 248/302 (82.12%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           MD RLLEIHP+ELKFI E NKHSLCSIQLTNN L+HVAFKVK TSP KY+VRPKVGIIEP
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLEHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
           NSTREFTVRMQAQKLA PDMLCKD+FLIQSTVVPADTTDEDITSSLF+K+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAM-- 180
           GVTLVSP ISQ       A +EEE  LGVT PNNL  AVEEIV+QEP +++HV SN +  
Sbjct: 121 GVTLVSPSISQ-------ALKEEEA-LGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDS 180

Query: 181 --------------------EPEPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLC 240
                               EPEPASLAND +DG+ E+LE+IK LEELKLKLSELELKLC
Sbjct: 181 LPPKHMVPGNGTDPRDVGEEEPEPASLANDVIDGDLENLEIIKDLEELKLKLSELELKLC 240

Query: 241 QAQNAISKLKEEKQISIQETKLLQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRF 281
           +AQNAISKLKEEKQISIQE K LQENFSELRKTEL  VQVGFPVLYLYMVALVCTFLGR 
Sbjct: 241 EAQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRL 294

BLAST of ClCG03G016540 vs. TAIR 10
Match: AT1G08820.1 (vamp/synaptobrevin-associated protein 27-2 )

HSP 1 Score: 189.1 bits (479), Expect = 4.8e-48
Identity = 143/385 (37.14%), Postives = 187/385 (48.57%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M+  LL+I PR L+F  +L K + C +QLTN T  +VAFKVK TSPKKY VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
            ST EFTV MQA K  PPDM+CKDKFLIQST V A+TTDEDIT+S+FSK  GK+IEE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKR----EEEVFLG------------------VTEPN----- 180
            VTLV P  S   SP++  K+    E+ +                     V EP      
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 ----------------------------NLCKAVEEIVEQE------------------- 240
                                       N  KA ++  +                     
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 --------------------PLNKNHVPSNAMEPEP-ASLANDFV-----DGNG----ED 281
                               P ++      A++ EP  S   D V     DG      ++
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

BLAST of ClCG03G016540 vs. TAIR 10
Match: AT1G08820.2 (vamp/synaptobrevin-associated protein 27-2 )

HSP 1 Score: 189.1 bits (479), Expect = 4.8e-48
Identity = 143/385 (37.14%), Postives = 187/385 (48.57%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M+  LL+I PR L+F  +L K + C +QLTN T  +VAFKVK TSPKKY VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
            ST EFTV MQA K  PPDM+CKDKFLIQST V A+TTDEDIT+S+FSK  GK+IEE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKR----EEEVFLG------------------VTEPN----- 180
            VTLV P  S   SP++  K+    E+ +                     V EP      
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 ----------------------------NLCKAVEEIVEQE------------------- 240
                                       N  KA ++  +                     
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 --------------------PLNKNHVPSNAMEPEP-ASLANDFV-----DGNG----ED 281
                               P ++      A++ EP  S   D V     DG      ++
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

BLAST of ClCG03G016540 vs. TAIR 10
Match: AT3G60600.1 (vesicle associated protein )

HSP 1 Score: 149.4 bits (376), Expect = 4.2e-36
Identity = 100/279 (35.84%), Postives = 140/279 (50.18%), Query Frame = 0

Query: 2   DTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEPN 61
           ++ LL + P +L+F FEL K   CS+ LTN T ++VAFKVK T+PKKY VRP  G++ P 
Sbjct: 19  NSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPR 78

Query: 62  STREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLG 121
           ST E  V MQAQK AP DM CKDKFL+Q  +     T +++T  +FSKE G  +EE KL 
Sbjct: 79  STCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLR 138

Query: 122 VTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAMEPE 181
           VT V+PP  +  SPV E   E                                       
Sbjct: 139 VTYVAPP--RPPSPVHEGSEEGS------------------------------------S 198

Query: 182 PASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKLL 241
           P +  +D  +G+G +    + + + K    E      +A+  I+KL EEKQ +IQ    L
Sbjct: 199 PRASVSD--NGHGSEFSFERFIVDNKAGHQE---NTSEARALITKLTEEKQSAIQLNNRL 254

Query: 242 QENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           Q    +LR+    S   G P +Y+ +V L+   LG  ++
Sbjct: 259 QRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIMK 254

BLAST of ClCG03G016540 vs. TAIR 10
Match: AT2G45140.1 (plant VAP homolog 12 )

HSP 1 Score: 142.9 bits (359), Expect = 3.9e-34
Identity = 96/280 (34.29%), Postives = 134/280 (47.86%), Query Frame = 0

Query: 1   MDTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEP 60
           M   LL I P +L+F FEL K   CS+ L N T ++VAFKVK T+PKKY VRP  G++ P
Sbjct: 1   MSNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHP 60

Query: 61  NSTREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKL 120
            S+ E  V MQAQK AP D+ CKDKFL+Q  V     T +D+T  +FSKE G  +EE KL
Sbjct: 61  RSSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKL 120

Query: 121 GVTLVSPPISQVFSPVDEAKREEEVFLGVTEPNNLCKAVEEIVEQEPLNKNHVPSNAMEP 180
            V  V+PP  +  SPV E   E                                      
Sbjct: 121 RVVYVAPP--RPPSPVREGSEEGS------------------------------------ 180

Query: 181 EPASLANDFVDGNGEDLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKL 240
            P +  +D  +GN  D          ++   +      +A+  ++KL EEK  ++Q    
Sbjct: 181 SPRASVSD--NGNASDFTAAPRFSADRVDAQD---NSSEARALVTKLTEEKNSAVQLNNR 237

Query: 241 LQENFSELRKTELNSVQVGFPVLYLYMVALVCTFLGRFLR 281
           LQ+   +LR+    S   G P +Y+ +V L+   LG  ++
Sbjct: 241 LQQELDQLRRESKRSKSGGIPFMYVLLVGLIGLILGYIMK 237

BLAST of ClCG03G016540 vs. TAIR 10
Match: AT3G60600.2 (vesicle associated protein )

HSP 1 Score: 137.9 bits (346), Expect = 1.3e-32
Identity = 72/141 (51.06%), Postives = 92/141 (65.25%), Query Frame = 0

Query: 2   DTRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYYVRPKVGIIEPN 61
           ++ LL + P +L+F FEL K   CS+ LTN T ++VAFKVK T+PKKY VRP  G++ P 
Sbjct: 19  NSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPR 78

Query: 62  STREFTVRMQAQKLAPPDMLCKDKFLIQSTVVPADTTDEDITSSLFSKEGGKYIEEQKLG 121
           ST E  V MQAQK AP DM CKDKFL+Q  +     T +++T  +FSKE G  +EE KL 
Sbjct: 79  STCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLR 138

Query: 122 VTLVSPPISQVFSPVDEAKRE 143
           VT V+PP  +  SPV E   E
Sbjct: 139 VTYVAPP--RPPSPVHEGSEE 157

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878362.14.2e-13187.15vesicle-associated protein 1-3-like isoform X1 [Benincasa hispida][more]
XP_011659931.19.7e-12081.94vesicle-associated protein 2-1 [Cucumis sativus] >XP_011659932.1 vesicle-associa... [more]
XP_008450729.12.2e-11981.60PREDICTED: vesicle-associated protein 1-3-like [Cucumis melo][more]
KAG7023965.14.7e-11477.81Vesicle-associated protein 2-2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022961473.18.0e-11477.15vesicle-associated protein 2-1-like isoform X3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
B9DHD76.7e-4737.14Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=... [more]
Q8VZ955.9e-3535.84Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=... [more]
Q9SHC85.5e-3334.29Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=... [more]
Q84WW51.8e-3137.77Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=... [more]
Q9LVU12.0e-3048.46Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LZ534.7e-12081.94MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G573020 PE=4 SV... [more]
A0A1S3BPW91.1e-11981.60vesicle-associated protein 1-3-like OS=Cucumis melo OX=3656 GN=LOC103492221 PE=4... [more]
A0A6J1HAA23.9e-11477.15vesicle-associated protein 2-1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HBX83.9e-11477.15vesicle-associated protein 2-1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1I0M11.2e-11276.49vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G08820.14.8e-4837.14vamp/synaptobrevin-associated protein 27-2 [more]
AT1G08820.24.8e-4837.14vamp/synaptobrevin-associated protein 27-2 [more]
AT3G60600.14.2e-3635.84vesicle associated protein [more]
AT2G45140.13.9e-3434.29plant VAP homolog 12 [more]
AT3G60600.21.3e-3251.06vesicle associated protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 203..244
NoneNo IPR availablePANTHERPTHR10809:SF45VESICLE-ASSOCIATED PROTEIN 2-2coord: 1..279
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 6..109
e-value: 1.9E-27
score: 95.3
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 5..125
score: 28.154163
IPR016763Vesicle-associated membrane-protein-associated proteinPIRSFPIRSF019693VAMP_assoc_protcoord: 1..280
e-value: 3.4E-47
score: 159.7
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 1..279
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 1..128
e-value: 4.6E-38
score: 132.0
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 3..132

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG03G016540.1ClCG03G016540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane