ClCG03G015140 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG03G015140
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description(1->3)-beta-glucan endohydrolase
LocationCG_Chr03: 30098255 .. 30098723 (+)
RNA-Seq ExpressionClCG03G015140
SyntenyClCG03G015140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAAGCTGATTGTCCTCTTCATGGTTGCCACGTCATCCATGGTCGGAGCATACGAGGTTCTTCTCGGCGCTTACTATGGACTTGCTGGCAACAACCTGCCACCACCATGGAAGGTGGTGCCAACTGTGTCGAAAATACAACATTCGTCGGGTTCGTTTGAATGAACCAAAGCTCGATGTTGTCGAAGCCTTTCCAGGCATGGGAATTGACCTCTCTTTTGGTGTGTCCAACAGCTTGATAACAAACATGGCCACCAATCACACCATACCTACATCGCACCCTTCATCCGCGATTTCACCATCAACTACATCATTATTGTTGGTCACGGAATCCCTTCAAAATCTCCTCAATGCTCGTTACCTCGGACAAGTGAAAACCACCACATTGGTTGGCTTATCCTCATTGGCAGTTAAAACCCCTCCATCTAGTGGCGCGTTTGACACCCCACCATTTCCAAGAACATGA

mRNA sequence

ATGGCAAAGCTGATTGTCCTCTTCATGGTTGCCACGTCATCCATGGTCGGAGCATACGAGGTTCTTCTCGGCGCTTACTATGGACTTGCTGGCAACAACCTGCCACCACCATGGAAGGTGGTGCCAACTGTGTCGAAAATACAACATTCGTCGGGCATGGGAATTGACCTCTCTTTTGGTGTGTCCAACAGCTTGATAACAAACATGGCCACCAATCACACCATACCTACATCGCACCCTTCATCCGCGATTTCACCATCAACTACATCATTATTGTTGGTCACGGAATCCCTTCAAAATCTCCTCAATGCTCGTTACCTCGGACAAGTGAAAACCACCACATTGGTTGGCTTATCCTCATTGGCAGTTAAAACCCCTCCATCTAGTGGCGCGTTTGACACCCCACCATTTCCAAGAACATGA

Coding sequence (CDS)

ATGGCAAAGCTGATTGTCCTCTTCATGGTTGCCACGTCATCCATGGTCGGAGCATACGAGGTTCTTCTCGGCGCTTACTATGGACTTGCTGGCAACAACCTGCCACCACCATGGAAGGTGGTGCCAACTGTGTCGAAAATACAACATTCGTCGGGCATGGGAATTGACCTCTCTTTTGGTGTGTCCAACAGCTTGATAACAAACATGGCCACCAATCACACCATACCTACATCGCACCCTTCATCCGCGATTTCACCATCAACTACATCATTATTGTTGGTCACGGAATCCCTTCAAAATCTCCTCAATGCTCGTTACCTCGGACAAGTGAAAACCACCACATTGGTTGGCTTATCCTCATTGGCAGTTAAAACCCCTCCATCTAGTGGCGCGTTTGACACCCCACCATTTCCAAGAACATGA

Protein sequence

MAKLIVLFMVATSSMVGAYEVLLGAYYGLAGNNLPPPWKVVPTVSKIQHSSGMGIDLSFGVSNSLITNMATNHTIPTSHPSSAISPSTTSLLLVTESLQNLLNARYLGQVKTTTLVGLSSLAVKTPPSSGAFDTPPFPRT
Homology
BLAST of ClCG03G015140 vs. NCBI nr
Match: XP_038880685.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida])

HSP 1 Score: 129.4 bits (324), Expect = 2.4e-26
Identity = 79/167 (47.31%), Postives = 103/167 (61.68%), Query Frame = 0

Query: 1   MAKLIVLFMVATSSMVGAYEVLLGAYYGLAGNNLPPPWKVV---------------PTVS 60
           ++ +IV+FMV  SS+VGAY+VLLGAYY LAGNNLPPPW+VV               P ++
Sbjct: 4   LSHIIVIFMVFVSSIVGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNLT 63

Query: 61  KIQHSSGMGIDLSFGVSNSLITNMATNHTIPTSHPSSAISPSTTS--------------- 120
            ++   GMGID+SFGV N+LI NMATNHT       + ++P                   
Sbjct: 64  VLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIPG 123

Query: 121 ----LLLVTESLQNLLNARYLGQVKTTTLVGLSSLAVKTPPSSGAFD 134
               +L V +SLQ LLNAR LGQVK TTLV +++L+ ++PPSSGAFD
Sbjct: 124 LDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFD 170

BLAST of ClCG03G015140 vs. NCBI nr
Match: TYK10032.1 (lichenase-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 126.3 bits (316), Expect = 2.0e-25
Identity = 83/169 (49.11%), Postives = 100/169 (59.17%), Query Frame = 0

Query: 4   LIVLFMVATSS-MVGAYEVLLGAYYGLAGNNLPPPWKVV---------------PTVSKI 63
           + +LFMVA SS +VGAY +LLGAYYGL  NNLP PWKV+               P +  I
Sbjct: 9   IALLFMVAMSSIVVGAYNILLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRIDEPNLDVI 68

Query: 64  QHSSGMGIDLSFGVSNSLITNMATNHTIPTSHPSSAISP-------------------ST 123
           +   G  I++SFGVSN LITNMATNHT      ++ I+P                     
Sbjct: 69  ESFRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVGDKAVPGLD 128

Query: 124 TSLLLVTESLQNLLNARYLGQVKTTTLVGLSSLAVKTPPSSGAFDTPPF 138
            S+L V +SLQ+LLN R+LGQVK TTLVGLS+L VK PPSSGAFD   F
Sbjct: 129 NSILPVMKSLQDLLNRRFLGQVKITTLVGLSALGVKAPPSSGAFDPNVF 177

BLAST of ClCG03G015140 vs. NCBI nr
Match: KAA0055781.1 (lichenase-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 124.0 bits (310), Expect = 1.0e-24
Identity = 83/169 (49.11%), Postives = 99/169 (58.58%), Query Frame = 0

Query: 4   LIVLFMVATSSMV-GAYEVLLGAYYGLAGNNLPPPWKVV---------------PTVSKI 63
           + +LFMV  SS+V GAY VLLGAYYGL  NNLP PWKV+               P +  I
Sbjct: 9   IALLFMVDMSSIVLGAYNVLLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRIDEPNLDVI 68

Query: 64  QHSSGMGIDLSFGVSNSLITNMATNHTIPTSHPSSAISP-------------------ST 123
           +   G  I++SFGVSN LITNMATNHT      ++ I+P                     
Sbjct: 69  ESFRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVGDKAVPGLD 128

Query: 124 TSLLLVTESLQNLLNARYLGQVKTTTLVGLSSLAVKTPPSSGAFDTPPF 138
            S+L V +SLQ+LLN R+LGQVK TTLVGLS+L VK PPSSGAFD   F
Sbjct: 129 DSILPVMKSLQDLLNRRFLGQVKITTLVGLSALGVKAPPSSGAFDPNVF 177

BLAST of ClCG03G015140 vs. NCBI nr
Match: KAA0055782.1 (lichenase-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 110.9 bits (276), Expect = 8.8e-21
Identity = 77/164 (46.95%), Postives = 92/164 (56.10%), Query Frame = 0

Query: 5   IVLFMVATSS-MVGAYEVLLGAYYGLAGNNLPPPWKVV---------------PTVSKIQ 64
           ++ FMVA SS +V AY+VLLGAYYG  GNNLPPP KVV               P    I 
Sbjct: 11  LLYFMVAMSSIVVRAYDVLLGAYYGTEGNNLPPPEKVVQLCQKYNIRRIRFSEPNFDIID 70

Query: 65  HSSGMGIDLSFGVSNSLITNMATNHTIPTSHPSSAISP-------------------STT 124
              G  I+LSF V+  LITNMATNHT       + ++P                      
Sbjct: 71  AFRGKDIELSFSVTGELITNMATNHTAVEEWFVNYVAPFIGDFTINYIIVGDKAIPGLDD 130

Query: 125 SLLLVTESLQNLLNARYLGQVKTTTLVGLSSLAVKTPPSSGAFD 134
           ++L V +SLQ LLN RYLGQVK TTLVGL++L V+TPPSSG FD
Sbjct: 131 NILPVMKSLQVLLNGRYLGQVKITTLVGLAALGVQTPPSSGTFD 174

BLAST of ClCG03G015140 vs. NCBI nr
Match: TYK10033.1 (lichenase-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 110.9 bits (276), Expect = 8.8e-21
Identity = 77/164 (46.95%), Postives = 92/164 (56.10%), Query Frame = 0

Query: 5   IVLFMVATSS-MVGAYEVLLGAYYGLAGNNLPPPWKVV---------------PTVSKIQ 64
           ++ FMVA SS +V AY+VLLGAYYG  GNNLPPP KVV               P    I 
Sbjct: 11  LLYFMVAMSSIVVRAYDVLLGAYYGTEGNNLPPPEKVVQLCQKYNIRRIRFSEPNFDIID 70

Query: 65  HSSGMGIDLSFGVSNSLITNMATNHTIPTSHPSSAISP-------------------STT 124
              G  I+LSF V+  LITNMATNHT       + ++P                      
Sbjct: 71  AFRGKDIELSFSVTGELITNMATNHTAVEEWFVNYVAPFIGDFTINYIIVGDKAIPGLDD 130

Query: 125 SLLLVTESLQNLLNARYLGQVKTTTLVGLSSLAVKTPPSSGAFD 134
           ++L V +SLQ LLN RYLGQVK TTLVGL++L V+TPPSSG FD
Sbjct: 131 NILPVMKSLQVLLNGRYLGQVKITTLVGLAALGVQTPPSSGTFD 174

BLAST of ClCG03G015140 vs. ExPASy TrEMBL
Match: A0A5D3CFE1 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001650 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 9.8e-26
Identity = 83/169 (49.11%), Postives = 100/169 (59.17%), Query Frame = 0

Query: 4   LIVLFMVATSS-MVGAYEVLLGAYYGLAGNNLPPPWKVV---------------PTVSKI 63
           + +LFMVA SS +VGAY +LLGAYYGL  NNLP PWKV+               P +  I
Sbjct: 9   IALLFMVAMSSIVVGAYNILLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRIDEPNLDVI 68

Query: 64  QHSSGMGIDLSFGVSNSLITNMATNHTIPTSHPSSAISP-------------------ST 123
           +   G  I++SFGVSN LITNMATNHT      ++ I+P                     
Sbjct: 69  ESFRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVGDKAVPGLD 128

Query: 124 TSLLLVTESLQNLLNARYLGQVKTTTLVGLSSLAVKTPPSSGAFDTPPF 138
            S+L V +SLQ+LLN R+LGQVK TTLVGLS+L VK PPSSGAFD   F
Sbjct: 129 NSILPVMKSLQDLLNRRFLGQVKITTLVGLSALGVKAPPSSGAFDPNVF 177

BLAST of ClCG03G015140 vs. ExPASy TrEMBL
Match: A0A5A7UQE4 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001680 PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 4.9e-25
Identity = 83/169 (49.11%), Postives = 99/169 (58.58%), Query Frame = 0

Query: 4   LIVLFMVATSSMV-GAYEVLLGAYYGLAGNNLPPPWKVV---------------PTVSKI 63
           + +LFMV  SS+V GAY VLLGAYYGL  NNLP PWKV+               P +  I
Sbjct: 9   IALLFMVDMSSIVLGAYNVLLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRIDEPNLDVI 68

Query: 64  QHSSGMGIDLSFGVSNSLITNMATNHTIPTSHPSSAISP-------------------ST 123
           +   G  I++SFGVSN LITNMATNHT      ++ I+P                     
Sbjct: 69  ESFRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVGDKAVPGLD 128

Query: 124 TSLLLVTESLQNLLNARYLGQVKTTTLVGLSSLAVKTPPSSGAFDTPPF 138
            S+L V +SLQ+LLN R+LGQVK TTLVGLS+L VK PPSSGAFD   F
Sbjct: 129 DSILPVMKSLQDLLNRRFLGQVKITTLVGLSALGVKAPPSSGAFDPNVF 177

BLAST of ClCG03G015140 vs. ExPASy TrEMBL
Match: A0A5A7UIV1 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001690 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 4.3e-21
Identity = 77/164 (46.95%), Postives = 92/164 (56.10%), Query Frame = 0

Query: 5   IVLFMVATSS-MVGAYEVLLGAYYGLAGNNLPPPWKVV---------------PTVSKIQ 64
           ++ FMVA SS +V AY+VLLGAYYG  GNNLPPP KVV               P    I 
Sbjct: 11  LLYFMVAMSSIVVRAYDVLLGAYYGTEGNNLPPPEKVVQLCQKYNIRRIRFSEPNFDIID 70

Query: 65  HSSGMGIDLSFGVSNSLITNMATNHTIPTSHPSSAISP-------------------STT 124
              G  I+LSF V+  LITNMATNHT       + ++P                      
Sbjct: 71  AFRGKDIELSFSVTGELITNMATNHTAVEEWFVNYVAPFIGDFTINYIIVGDKAIPGLDD 130

Query: 125 SLLLVTESLQNLLNARYLGQVKTTTLVGLSSLAVKTPPSSGAFD 134
           ++L V +SLQ LLN RYLGQVK TTLVGL++L V+TPPSSG FD
Sbjct: 131 NILPVMKSLQVLLNGRYLGQVKITTLVGLAALGVQTPPSSGTFD 174

BLAST of ClCG03G015140 vs. ExPASy TrEMBL
Match: A0A5D3CDU1 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001660 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 4.3e-21
Identity = 77/164 (46.95%), Postives = 92/164 (56.10%), Query Frame = 0

Query: 5   IVLFMVATSS-MVGAYEVLLGAYYGLAGNNLPPPWKVV---------------PTVSKIQ 64
           ++ FMVA SS +V AY+VLLGAYYG  GNNLPPP KVV               P    I 
Sbjct: 11  LLYFMVAMSSIVVRAYDVLLGAYYGTEGNNLPPPEKVVQLCQKYNIRRIRFSEPNFDIID 70

Query: 65  HSSGMGIDLSFGVSNSLITNMATNHTIPTSHPSSAISP-------------------STT 124
              G  I+LSF V+  LITNMATNHT       + ++P                      
Sbjct: 71  AFRGKDIELSFSVTGELITNMATNHTAVEEWFVNYVAPFIGDFTINYIIVGDKAIPGLDD 130

Query: 125 SLLLVTESLQNLLNARYLGQVKTTTLVGLSSLAVKTPPSSGAFD 134
           ++L V +SLQ LLN RYLGQVK TTLVGL++L V+TPPSSG FD
Sbjct: 131 NILPVMKSLQVLLNGRYLGQVKITTLVGLAALGVQTPPSSGTFD 174

BLAST of ClCG03G015140 vs. ExPASy TrEMBL
Match: A0A6J1C4V7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007943 PE=3 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.1e-20
Identity = 75/170 (44.12%), Postives = 94/170 (55.29%), Query Frame = 0

Query: 1   MAKL--IVLFMVATSSMVGAYEVLLGAYYGLAGNNLPPPWKVV---------------PT 60
           MAKL  I++F +A+ S V A +VLLG YYGL G+N+PPPWKVV               P 
Sbjct: 1   MAKLAHIIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPN 60

Query: 61  VSKIQHSSGMGIDLSFGVSNSLITNMATNHTIPTSHPSSAISP----------------- 120
           +       G GID+SFGV N ++ NM  N +      ++ + P                 
Sbjct: 61  LEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAI 120

Query: 121 --STTSLLLVTESLQNLLNARYLGQVKTTTLVGL-SSLAVKTPPSSGAFD 134
                 +L V +SLQNLLNARYLGQVK TTLVG  ++LAVK PPSSGAFD
Sbjct: 121 PGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFD 170

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880685.12.4e-2647.31probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida][more]
TYK10032.12.0e-2549.11lichenase-2-like [Cucumis melo var. makuwa][more]
KAA0055781.11.0e-2449.11lichenase-2-like [Cucumis melo var. makuwa][more]
KAA0055782.18.8e-2146.95lichenase-2-like [Cucumis melo var. makuwa][more]
TYK10033.18.8e-2146.95lichenase-2-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3CFE19.8e-2649.11(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7UQE44.9e-2549.11(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7UIV14.3e-2146.95(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5D3CDU14.3e-2146.95(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1C4V72.1e-2044.12(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007943 ... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 23..139
e-value: 9.9E-6
score: 26.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG03G015140.1ClCG03G015140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity