ClCG03G002740 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG03G002740
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	iron-sulfur assembly protein IscA-like 2, mitochondrial
Location	CG_Chr03: 3158346 .. 3161792 (-)
RNA-Seq Expression	ClCG03G002740
Synteny	ClCG03G002740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTGACAAAGCTGTCATCAATCCTGCTGTGGTCGTTTCCTTTGACACACTACTTTGCACATTCTTTGTCAAAGTTAGGCGAAATAGTCCCTAGAATTTTATGTCCAACTTTTTCCATTCAGCTTCTCCTATGTCATTAGGTTTTCCTTCCAATGACAAATCAAAATCATTGGAATGCAAGTAGTCTAATATTTGACATTTTCAAAAAGAAAAATCTGAACTATTAAAAACAACCAATTTTTCATCTCTATCTGACATCGTTCTTCAACCCAACCTAGCTTTAATACCACTTGATGGAAAATAATAATATATCAATCTAATATAATTCCATTTTAGTTTAACACTAGTTTTCATACGATTTTTTTTTTTTTTTTTGTTTAAACGATTTTTCTTGTTAAAAATAGTAAAATTGAGAAGAATATTGAATATAAATAACGTTACCATATTTAAACTTGGATAAAATTGAGGTTGGAAAGGCCTCTTTGTCGATCATAGAAAAATTTCCATTGCCCCAAATTTTTGTCAATCCAACTTCTCTCTTTCTCGATACCTTCTGTGTTCTTCCCTGATTCAATCCGAAACTTCGTCCCCTATCTTTATAATTTCCATCTTTCCTTCCATATTATCTCTTCCTTCTTGTTTGATTCCAATAGCTGCAGCTTCCTCCCCCTTTGTCAATTACTTTTGGTTTGAGTTGCGAACTTGCGACTGGGGAATGTGTGGAAGACAATGAACTACGAGCGCACCAAATTTTCTGCCGCGGGGTGATGACTTTTCAAGTAGAGTTATTGATGCTAACAACTTGCATTGGCAAAGAGGAGAATTGCTTATGCTCTTCTGTTTGCTGCCTCATCAGCATGTAAGAACCTTCTCCTTTTTCCTTCTATTAATATTTTTTCTGCTTGTTTTCCTTCTTTTGCTTACAATTTCTTCTGTTTGGGATTGGTTTCCAGCTGCTTTTTTTCCCCTTCTGTTTGGGATTGATTACTCCTTACTTTGAAACTGAAAATACGTAACATTATTAAACCTCGTGAGGGTTGGATATTCCTTTTGAATTGATTTGGGTTCTCAATTCTCCCTAATTGAATGGATGTTTGGCTTTTGGAAATCCTTGCGGGTATGATGTTTATTGGCAAGGCTAAGATTCTTCGGAATTTCACAGTTAGAGCTCTTCTTGGGCTGACTTGGAAAGGGAGAGAAGTCACAGGCTTTTTGTAGACAAGATGGTTTTAGAGAAACTCTTTTGTTAGAACTGCCTTCTCATGATTCTCTTTTCTCTCTGGTTGAAAGGCTCTTCTGCTTAGTGTGCTTGTTGGGAAAGAGTTTTAGGTCTAACCAATTGTAGTTATTTGCATGTTATATAAGTTGTACATTAAGTTTTCATATGTGTGGGATCTCATTATCTTTTAACCTCCCATTGGGCTTATTATCTTCAACTCTTGTAATCTAATTGGGAAACGATCTTGTGTTGCTAGCATTTTGGGACTACTTTTTCCCTCTGTTTTGGTATTTTGTTTATCGTTGAAATTGGGAAAAAAGTAGCTAACAATGTAGTGATCTAATCGGTTGAAGTTTCATAGGTAACATCATTTTTGTATATCAATTTTATATGAGTCCTTAAGGATTTAATTTCTCATTATTCTATATCCATATGATTTTGAGTGAATTAGAATAGAAATTGCTTTCCAAGAAAATGTTCTAACGCTGCAAAGTTTCTGGATTCATGTGTTGTGTTTGAATATTGCCTGCCACAATACTTTATCGAAATTATCTGGGTCAATTTGAAATCTTGGATTTCCATTTTTGTTTTAACTTTTAATTGACCCCGATACCCTTGAAGAAGCATTGTCCTTATTAGTCATGTTAATGCCTTTAGAATTCGGTTTTGCGGCAGATAAGGATCAAATTTCAAGTGGAGAGAGTAAAGAGATGGTCCCCAGATTTCTTATTCAGCGGCTAACACCATACTTGGCAACACGTATCCGAGAGAACCAAAGGCTTCTTAGCTCTTCTGTTTCAGCTCATCAAGAAGCCTCTTCTTCGTTGATAACTCCATCTACGGGCACTGATACCATCCACATGACAGAAAATTGCATTCGGGTATCCCTACATTTTATGCAATCCTTTGTGTGCATGTACCATATTGATTAGAAATTAGTCATCTATTTCACTGTCGTCATTGTTTTAGTTCTTAATTTTCTGGACAATCCTCATTTTGTGCACTATTTGGGCCTATAATTTCTTGGTTTTAGGATGCTAATGAACTATTGCCTTTAATATTGGTAATTTGAGCGAAGGATATGAAAATTGGGAATTTTCTTGTTATCACTTCAAATTTGGGGATTTTCTTTGTCATATATGATGATTTAAAGCGAGGCTTAAGTTATAATGCCAAAACTGGAGAAGTATTCAGAATAATATGGTGCCAGTTGCAACAATTATCTTATTCTTAATTTTAAAAAAATTTTACTTGAAAGTTGGAATTTTTGAAAGGGACTGTGACTTGTAAACTTAGGAATGAACTCTTCTTAACTAGATGTATGATGTATTCTCTGTTACCAAAGGGCTAAATTGGTTGATACTCCTATTTATGTTATTGATAAAGGAATATATCAATAAGAGAGTCACAGGTCTTCATCTTCTAAAAGCATTCATTCTATCAGTTTTCTACATCATTTTTTCCCCTTTTCTGCTGCATGTAAGGGTTCAATGATGAAGTTTTTATGAAGAACGGTCTTGGTTTACTCTCTCCTGAGTCTAGACATTCCTTGCAGAGAATGAAAGAATTGCAAGAACCCACGGAAGAAAAGATGCTCCGCTTGAGTGTAGAAAATGGAGGATGTTCTGGATTTCAATATGTTTTCAATTTGGATGACAAAACCAATTCAGATGACAGGTCTATAAAATAGCTCCAATTCACTGCTTATTTTGGATTTTATCTCAAAGATTGCCAGTGATGTTTGGTTTCACTTTTTAAAACTTTACCCTTCTCTCTCCCTTCATGTTTGCAGGGTGTGTGAAAAGGAGGGAGTTAAATTGGTAGTTGATAATATCTCATATGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAAGAGCTGATTCGTTCCGCTTTCATTGTGAGTAAAACCTATATCTCAGTATTTCTGGTTCACCATTTTTGCTTAGATATGCTTATTACTTTTATACCAGAGTGGCCCCTAACAAACCATAGTTATATGACATCTACAAACCCAATGGTCTGTGATCTGTACCAAGTAGTAAAATGCTCCAGAAGCCTCATTTTACGCCATATATTTTATGCATTCTTAAATCTTATCTCACAATAAAACTAGTGCTACTGCAGAAGTTGAACTCTTATATTATGCCTATGTGCAGGTGACCACAAATCCAAGTGCTGTTGGGGGCTGTAGTTGTAAAAGTTCTTTCATGGTGAAACAATAA

mRNA sequence

ATGTTTGACAAAGCTGTCATCAATCCTGCTGTGGTCGTTTCCTTTGACACACTACTTTGCACATTCTTTGTCAAAAGGAGAATTGCTTATGCTCTTCTGTTTGCTGCCTCATCAGCATATAAGGATCAAATTTCAAGTGGAGAGAGTAAAGAGATGGTCCCCAGATTTCTTATTCAGCGGCTAACACCATACTTGGCAACACGTATCCGAGAGAACCAAAGGCTTCTTAGCTCTTCTGTTTCAGCTCATCAAGAAGCCTCTTCTTCGTTGATAACTCCATCTACGGGCACTGATACCATCCACATGACAGAAAATTGCATTCGGAGAATGAAAGAATTGCAAGAACCCACGGAAGAAAAGATGCTCCGCTTGAGTGTAGAAAATGGAGGATGTTCTGGATTTCAATATGTTTTCAATTTGGATGACAAAACCAATTCAGATGACAGGGTGTGTGAAAAGGAGGGAGTTAAATTGGTAGTTGATAATATCTCATATGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAAGAGCTGATTCGTTCCGCTTTCATTGTGACCACAAATCCAAGTGCTGTTGGGGGCTGTAGTTGTAAAAGTTCTTTCATGGTGAAACAATAA

Coding sequence (CDS)

ATGTTTGACAAAGCTGTCATCAATCCTGCTGTGGTCGTTTCCTTTGACACACTACTTTGCACATTCTTTGTCAAAAGGAGAATTGCTTATGCTCTTCTGTTTGCTGCCTCATCAGCATATAAGGATCAAATTTCAAGTGGAGAGAGTAAAGAGATGGTCCCCAGATTTCTTATTCAGCGGCTAACACCATACTTGGCAACACGTATCCGAGAGAACCAAAGGCTTCTTAGCTCTTCTGTTTCAGCTCATCAAGAAGCCTCTTCTTCGTTGATAACTCCATCTACGGGCACTGATACCATCCACATGACAGAAAATTGCATTCGGAGAATGAAAGAATTGCAAGAACCCACGGAAGAAAAGATGCTCCGCTTGAGTGTAGAAAATGGAGGATGTTCTGGATTTCAATATGTTTTCAATTTGGATGACAAAACCAATTCAGATGACAGGGTGTGTGAAAAGGAGGGAGTTAAATTGGTAGTTGATAATATCTCATATGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAAGAGCTGATTCGTTCCGCTTTCATTGTGACCACAAATCCAAGTGCTGTTGGGGGCTGTAGTTGTAAAAGTTCTTTCATGGTGAAACAATAA

Protein sequence

MFDKAVINPAVVVSFDTLLCTFFVKRRIAYALLFAASSAYKDQISSGESKEMVPRFLIQRLTPYLATRIRENQRLLSSSVSAHQEASSSLITPSTGTDTIHMTENCIRRMKELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ

Homology

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BLAST of ClCG03G002740 vs. NCBI nr
Match: XP_038895337.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 297.4 bits (760), Expect = 9.8e-77
Identity = 151/175 (86.29%), Postives = 159/175 (90.86%), Query Frame = 0


Query: 32  LLFAASSAYKDQISSGESKEMVPRFLIQRLTPYLATRIRENQRLLSSSVSAHQEASSSLI 91
           L +  SS  ++QIS G +KEMVPRFLIQRLTPY A RIRENQRLLSSSV AHQE SSSLI
Sbjct: 8   LRYLKSSLDENQISRGVNKEMVPRFLIQRLTPYFAARIRENQRLLSSSVLAHQETSSSLI 67

Query: 92  TPSTGTDTIHMTENCIRRMKELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKTNSDDRVC 151
           TPS G DTIHMT+NCIRRMKELQEP EEKMLRLSVE GGCSGFQYVFNLD+KTN DDRVC
Sbjct: 68  TPSVGIDTIHMTDNCIRRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDEKTNPDDRVC 127

Query: 152 EKEGVKLVVDNISYDFVKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 207
           EK+GVKLVVDNISYDFVKGATIDYVEELIRSAF+VTTNPSAVGGCSCKSSFMVKQ
Sbjct: 128 EKQGVKLVVDNISYDFVKGATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 182

[Close]

BLAST of ClCG03G002740 vs. NCBI nr
Match: XP_004147288.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [Cucumis sativus] >KGN64807.2 hypothetical protein Csa_013472 [Cucumis sativus])

HSP 1 Score: 285.4 bits (729), Expect = 3.9e-73
Identity = 144/155 (92.90%), Postives = 148/155 (95.48%), Query Frame = 0


Query: 52  MVPRFLIQRLTPYLATRIRENQRLLSSSVSAHQEASSSLITPSTGTDTIHMTENCIRRMK 111
           MVPRFLIQRLTPYL   IR+NQRLLSSSVSA+QEASSSLITPS G DTIHM++NCIRRMK
Sbjct: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEASSSLITPSAGIDTIHMSDNCIRRMK 60

Query: 112 ELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDFVKGA 171
           ELQEP EEKMLRLSVENGGCSGFQYVFNLDDKTN DDRVCEKEGVKLVVDNISYDFVKGA
Sbjct: 61  ELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVKGA 120

Query: 172 TIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 207
           TIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 TIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 155

[Close]

BLAST of ClCG03G002740 vs. NCBI nr
Match: XP_038895338.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [Benincasa hispida] >XP_038895339.1 iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 283.5 bits (724), Expect = 1.5e-72
Identity = 142/155 (91.61%), Postives = 146/155 (94.19%), Query Frame = 0


Query: 52  MVPRFLIQRLTPYLATRIRENQRLLSSSVSAHQEASSSLITPSTGTDTIHMTENCIRRMK 111
           MVPRFLIQRLTPY A RIRENQRLLSSSV AHQE SSSLITPS G DTIHMT+NCIRRMK
Sbjct: 1   MVPRFLIQRLTPYFAARIRENQRLLSSSVLAHQETSSSLITPSVGIDTIHMTDNCIRRMK 60

Query: 112 ELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDFVKGA 171
           ELQEP EEKMLRLSVE GGCSGFQYVFNLD+KTN DDRVCEK+GVKLVVDNISYDFVKGA
Sbjct: 61  ELQEPMEEKMLRLSVETGGCSGFQYVFNLDEKTNPDDRVCEKQGVKLVVDNISYDFVKGA 120

Query: 172 TIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 207
           TIDYVEELIRSAF+VTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 TIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 155

[Close]

BLAST of ClCG03G002740 vs. NCBI nr
Match: XP_022998904.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 264.2 bits (674), Expect = 9.2e-67
Identity = 137/159 (86.16%), Postives = 142/159 (89.31%), Query Frame = 0


Query: 52  MVPRFLIQRLTPYLATRIRENQRLLSSSVSAHQEASSSLI----TPSTGTDTIHMTENCI 111
           MVPR L QRL PYLA RIRENQRLLSSSVSA QEASS L     +PS  TDTIHMT+NCI
Sbjct: 1   MVPRLLSQRLAPYLAARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60

Query: 112 RRMKELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDF 171
           RRMKELQEP EEKMLRLSVE GGCSGFQYVFNLDD+TN DDRVCEKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120

Query: 172 VKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 207
           VKGAT+DYVEELIR AF+VTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of ClCG03G002740 vs. NCBI nr
Match: XP_022948821.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita moschata] >KAG7036850.1 Iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 262.7 bits (670), Expect = 2.7e-66
Identity = 136/159 (85.53%), Postives = 141/159 (88.68%), Query Frame = 0


Query: 52  MVPRFLIQRLTPYLATRIRENQRLLSSSVSAHQEASSSLI----TPSTGTDTIHMTENCI 111
           MVPR L QRL PYL  RIRENQRLLSSSVSA QEASS L     +PS  TDTIHMT+NCI
Sbjct: 1   MVPRLLSQRLAPYLTARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60

Query: 112 RRMKELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDF 171
           RRMKELQEP EEKMLRLSVE GGCSGFQYVFNLDD+TN DDRVCEKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120

Query: 172 VKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 207
           VKGAT+DYVEELIR AF+VTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of ClCG03G002740 vs. ExPASy Swiss-Prot
Match: Q8LCY2 (Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g03905 PE=1 SV=2)

HSP 1 Score: 202.2 bits (513), Expect = 5.6e-51
Identity = 110/156 (70.51%), Postives = 127/156 (81.41%), Query Frame = 0


Query: 55  RFLIQRLTPYLATRIRENQRLLS-SSVSAHQEASSSLIT--PSTGTDTIHMTENCIRRMK 114
           R L++R+ PYLA RIREN RLL+ SS SA +EASSS  +   S+  D +H+++NCIRRMK
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSNDVVHLSDNCIRRMK 62

Query: 115 ELQ--EPTEEKMLRLSVENGGCSGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDFVK 174
           ELQ  EP E+KMLRL VE GGCSGFQY F LD++TN DDRV EK GVKLVVDN+SYD VK
Sbjct: 63  ELQSSEP-EKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDLVK 122

Query: 175 GATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVK 206
           GATIDY EELIR+AF+V  NPSAVGGCSCKSSFMVK
Sbjct: 123 GATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVK 157

[Close]

BLAST of ClCG03G002740 vs. ExPASy Swiss-Prot
Match: Q2TBG7 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus OX=9913 GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.3e-28
Identity = 68/135 (50.37%), Postives = 88/135 (65.19%), Query Frame = 0


Query: 71  ENQRLLSSSVSAHQEASSSLITPSTGTDTIHMTENCIRRMKELQEPTEEKMLRLSVENGG 130
           +  RLL++S         S  +P  G   IH+T++C++R+ E+ E +E   LRL VE GG
Sbjct: 19  QRSRLLAASRGPRALREVSFSSPEAGEGQIHLTDSCVQRLLEITEGSE--FLRLEVEGGG 78

Query: 131 CSGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFIVTTNP 190
           CSGFQY F+LD   N DDRV EK G ++VVD+ S  FVKGA +D+ +ELIRS+F V  NP
Sbjct: 79  CSGFQYKFSLDTVINPDDRVFEKGGARVVVDSDSLAFVKGAHVDFSQELIRSSFQVLNNP 138

Query: 191 SAVGGCSCKSSFMVK 206
            A  GCSC SSF VK
Sbjct: 139 QAQQGCSCGSSFSVK 151

[Close]

BLAST of ClCG03G002740 vs. ExPASy Swiss-Prot
Match: Q86U28 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens OX=9606 GN=ISCA2 PE=1 SV=2)

HSP 1 Score: 125.2 bits (313), Expect = 8.7e-28
Identity = 70/134 (52.24%), Postives = 93/134 (69.40%), Query Frame = 0


Query: 74  RLLSSSV--SAHQEASSSLITPSTGTDTIHMTENCIRRMKELQEPTEEKMLRLSVENGGC 133
           RLL++S+   A +EASSS  +P  G   I +T++C++R+ E+ E +E   LRL VE GGC
Sbjct: 24  RLLTASLGPQARREASSS--SPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGC 83

Query: 134 SGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFIVTTNPS 193
           SGFQY F+LD   N DDRV E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F V  NP 
Sbjct: 84  SGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQ 143

Query: 194 AVGGCSCKSSFMVK 206
           A  GCSC SSF +K
Sbjct: 144 AQQGCSCGSSFSIK 153

[Close]

BLAST of ClCG03G002740 vs. ExPASy Swiss-Prot
Match: Q5R788 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii OX=9601 GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 8.7e-28
Identity = 70/134 (52.24%), Postives = 93/134 (69.40%), Query Frame = 0


Query: 74  RLLSSSV--SAHQEASSSLITPSTGTDTIHMTENCIRRMKELQEPTEEKMLRLSVENGGC 133
           RLL++S+   A +EASSS  +P  G   I +T++C++R+ E+ E +E   LRL VE GGC
Sbjct: 24  RLLAASLGPQARREASSS--SPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGC 83

Query: 134 SGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFIVTTNPS 193
           SGFQY F+LD   N DDRV E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F V  NP 
Sbjct: 84  SGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQ 143

Query: 194 AVGGCSCKSSFMVK 206
           A  GCSC SSF +K
Sbjct: 144 AQQGCSCGSSFSIK 153

[Close]

BLAST of ClCG03G002740 vs. ExPASy Swiss-Prot
Match: Q9DCB8 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus OX=10090 GN=Isca2 PE=1 SV=2)

HSP 1 Score: 120.2 bits (300), Expect = 2.8e-26
Identity = 64/121 (52.89%), Postives = 83/121 (68.60%), Query Frame = 0


Query: 85  EASSSLITPSTGTDTIHMTENCIRRMKELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKT 144
           E +SS+  P  G   I +T++C++R+ E+ E +E   LRL VE GGCSGFQY F+LD   
Sbjct: 37  ETTSSI--PEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGCSGFQYKFSLDTVI 96

Query: 145 NSDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMV 204
           N DDRV E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F V  NP A  GCSC SSF V
Sbjct: 97  NPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQAQQGCSCGSSFSV 153

Query: 205 K 206
           K
Sbjct: 157 K 153

[Close]

BLAST of ClCG03G002740 vs. ExPASy TrEMBL
Match: A0A6J1KFL9 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111493424 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 4.5e-67
Identity = 137/159 (86.16%), Postives = 142/159 (89.31%), Query Frame = 0


Query: 52  MVPRFLIQRLTPYLATRIRENQRLLSSSVSAHQEASSSLI----TPSTGTDTIHMTENCI 111
           MVPR L QRL PYLA RIRENQRLLSSSVSA QEASS L     +PS  TDTIHMT+NCI
Sbjct: 1   MVPRLLSQRLAPYLAARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60

Query: 112 RRMKELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDF 171
           RRMKELQEP EEKMLRLSVE GGCSGFQYVFNLDD+TN DDRVCEKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120

Query: 172 VKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 207
           VKGAT+DYVEELIR AF+VTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of ClCG03G002740 vs. ExPASy TrEMBL
Match: A0A6J1GA98 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111452373 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.3e-66
Identity = 136/159 (85.53%), Postives = 141/159 (88.68%), Query Frame = 0


Query: 52  MVPRFLIQRLTPYLATRIRENQRLLSSSVSAHQEASSSLI----TPSTGTDTIHMTENCI 111
           MVPR L QRL PYL  RIRENQRLLSSSVSA QEASS L     +PS  TDTIHMT+NCI
Sbjct: 1   MVPRLLSQRLAPYLTARIRENQRLLSSSVSALQEASSLLTSPAQSPSPHTDTIHMTDNCI 60

Query: 112 RRMKELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDF 171
           RRMKELQEP EEKMLRLSVE GGCSGFQYVFNLDD+TN DDRVCEKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDQTNPDDRVCEKEGVKLVVDNISYDF 120

Query: 172 VKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 207
           VKGAT+DYVEELIR AF+VTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATVDYVEELIRCAFVVTTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of ClCG03G002740 vs. ExPASy TrEMBL
Match: A0A6J1DIX6 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111020448 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 5.4e-65
Identity = 132/159 (83.02%), Postives = 140/159 (88.05%), Query Frame = 0


Query: 52  MVPRFLIQRLTPYLATRIRENQRLLSSSVSAHQEASSSLITPSTGT----DTIHMTENCI 111
           MVPR LIQRLTP+   RIRENQRLLSSSVSAHQE  S L +PS  +    DTIHMT+NCI
Sbjct: 1   MVPRLLIQRLTPHFIARIRENQRLLSSSVSAHQEVPSLLTSPSPSSSVLIDTIHMTDNCI 60

Query: 112 RRMKELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDF 171
           ++MKELQEP EEKMLRLSVE GGCSGFQYVFNLDDKTN DDRV EK+GVKLVVDNISYDF
Sbjct: 61  QKMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDKTNPDDRVYEKDGVKLVVDNISYDF 120

Query: 172 VKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 207
           VKGATIDY EELIRSAF+VTTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYAEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of ClCG03G002740 vs. ExPASy TrEMBL
Match: A0A6J1HPD1 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111464825 PE=4 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 7.1e-65
Identity = 133/159 (83.65%), Postives = 141/159 (88.68%), Query Frame = 0


Query: 52  MVPRFLIQRLTPYLATRIRENQRLLSSSVSAHQEASSSLITPSTGT----DTIHMTENCI 111
           M PR LIQRLTP+L  RIRENQRLLSSSVSAHQEASS L +PS  +    DTI MT+NCI
Sbjct: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60

Query: 112 RRMKELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDF 171
           RRMKELQ P EEKMLRLSVE GGCSGFQYVF+LDDKTN DDRV EKEGV+LVVDNISYDF
Sbjct: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120

Query: 172 VKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 207
           VKGATIDYVEELIRSAF+V+TNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of ClCG03G002740 vs. ExPASy TrEMBL
Match: A0A6J1I3Y1 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111469443 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 6.0e-64
Identity = 132/159 (83.02%), Postives = 140/159 (88.05%), Query Frame = 0


Query: 52  MVPRFLIQRLTPYLATRIRENQRLLSSSVSAHQEASSSLITPSTGT----DTIHMTENCI 111
           M  R LIQRLTP+L  RIRENQRLLSSSVSAHQEASS L +PS  +    DTI MT+NCI
Sbjct: 1   MASRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60

Query: 112 RRMKELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDF 171
           RRMKELQ P EEKMLRLSVE GGCSGFQYVF+LDDKTN DDRV EKEGV+LVVDNISYDF
Sbjct: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120

Query: 172 VKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 207
           VKGATIDYVEELIRSAF+V+TNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of ClCG03G002740 vs. TAIR 10
Match: AT5G03905.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 202.2 bits (513), Expect = 4.0e-52
Identity = 110/156 (70.51%), Postives = 127/156 (81.41%), Query Frame = 0


Query: 55  RFLIQRLTPYLATRIRENQRLLS-SSVSAHQEASSSLIT--PSTGTDTIHMTENCIRRMK 114
           R L++R+ PYLA RIREN RLL+ SS SA +EASSS  +   S+  D +H+++NCIRRMK
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSNDVVHLSDNCIRRMK 62

Query: 115 ELQ--EPTEEKMLRLSVENGGCSGFQYVFNLDDKTNSDDRVCEKEGVKLVVDNISYDFVK 174
           ELQ  EP E+KMLRL VE GGCSGFQY F LD++TN DDRV EK GVKLVVDN+SYD VK
Sbjct: 63  ELQSSEP-EKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDLVK 122

Query: 175 GATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVK 206
           GATIDY EELIR+AF+V  NPSAVGGCSCKSSFMVK
Sbjct: 123 GATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVK 157

[Close]

BLAST of ClCG03G002740 vs. TAIR 10
Match: AT2G16710.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 70.1 bits (170), Expect = 2.4e-12
Identity = 36/118 (30.51%), Postives = 66/118 (55.93%), Query Frame = 0


Query: 86  ASSSLITPSTGTDTIHMTENCIRRMKELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKTN 145
           A+++ + P+     + +T+    R+  L +  ++  LRL V+  GC+G  Y  N  D+  
Sbjct: 9   AAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLNYADEKG 68

Query: 146 SDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFM 204
             D + E++GV+++V+  +   V G  +D+V++ +RS F V  NP++ G C C  SFM
Sbjct: 69  KFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEF-VFINPNSQGQCGCGESFM 125

[Close]

BLAST of ClCG03G002740 vs. TAIR 10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 65.9 bits (159), Expect = 4.5e-11
Identity = 36/101 (35.64%), Postives = 59/101 (58.42%), Query Frame = 0


Query: 102 MTENCIRRMKELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKTNSDDRVCEKEGVKLVVD 161
           +++    R+++L +  ++  LRL+VE  GC+G  YV N   +    D V E++GVK++VD
Sbjct: 8   LSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKGVKILVD 67

Query: 162 NISYDFVKGATIDYVEELIRSAFIVTTNPSAVGGCSCKSSF 203
             +   V G  +D+V++ +RS F V  NP+A   C C  SF
Sbjct: 68  PKAVMHVIGTEMDFVDDKLRSEF-VFVNPNAT-KCGCGESF 106

[Close]

BLAST of ClCG03G002740 vs. TAIR 10
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.2e-10
Identity = 42/142 (29.58%), Postives = 70/142 (49.30%), Query Frame = 0


Query: 64  YLATRIRENQRLLSSSVSAHQEASSSLITPSTGTDTIHMTENCIRRMKELQ-EPTEEKML 123
           Y+   +    RL   S S     +   + P+     I ++EN ++ + +++ E  E+  L
Sbjct: 41  YVNLNLNRRNRLSVRSASVPAAPAMEGLKPA-----ISLSENALKHLSKMRSERGEDLCL 100

Query: 124 RLSVENGGCSGFQYVFNLDDKTNS--DDRVCEKEGVKLVVDNISYDFVKGATIDYVEELI 183
           R+ V+ GGCSG  Y  + +++ N+  DD   E +G  +V D  S  F+ G  +DY + LI
Sbjct: 101 RIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYSDALI 160

Query: 184 RSAFIVTTNPSAVGGCSCKSSF 203
              F   +NP+A   C C  SF
Sbjct: 161 GGGFSF-SNPNATQTCGCGKSF 176

[Close]

BLAST of ClCG03G002740 vs. TAIR 10
Match: AT2G16710.2 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 53.1 bits (126), Expect = 3.0e-07
Identity = 25/96 (26.04%), Postives = 53/96 (55.21%), Query Frame = 0


Query: 86  ASSSLITPSTGTDTIHMTENCIRRMKELQEPTEEKMLRLSVENGGCSGFQYVFNLDDKTN 145
           A+++ + P+     + +T+    R+  L +  ++  LRL V+  GC+G  Y  N  D+  
Sbjct: 9   AAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLNYADEKG 68

Query: 146 SDDRVCEKEGVKLVVDNISYDFVKGATIDYVEELIR 182
             D + E++GV+++V+  +   V G  +D+V++ +R
Sbjct: 69  KFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLR 104

The following BLAST results are available for this feature:

BLAST of ClCG03G002740 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895337.1	9.8e-77	86.29	iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [Benincasa hi...	[more]
XP_004147288.1	3.9e-73	92.90	iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [Cucumis sati...	[more]
XP_038895338.1	1.5e-72	91.61	iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [Benincasa hi...	[more]
XP_022998904.1	9.2e-67	86.16	iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita maxima]	[more]
XP_022948821.1	2.7e-66	85.53	iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita moschata] >KA...	[more]

BLAST of ClCG03G002740 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LCY2	5.6e-51	70.51	Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana ...	[more]
Q2TBG7	2.3e-28	50.37	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus OX=9913 GN=I...	[more]
Q86U28	8.7e-28	52.24	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens OX=9606 GN...	[more]
Q5R788	8.7e-28	52.24	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii OX=9601 GN...	[more]
Q9DCB8	2.8e-26	52.89	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus OX=10090 G...	[more]

BLAST of ClCG03G002740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KFL9	4.5e-67	86.16	iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita maxima OX=3...	[more]
A0A6J1GA98	1.3e-66	85.53	iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita moschata OX...	[more]
A0A6J1DIX6	5.4e-65	83.02	iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Momordica charantia O...	[more]
A0A6J1HPD1	7.1e-65	83.65	iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita moschata OX...	[more]
A0A6J1I3Y1	6.0e-64	83.02	iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita maxima OX=3...	[more]

BLAST of ClCG03G002740 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03905.1	4.0e-52	70.51	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G16710.1	2.4e-12	30.51	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	4.5e-11	35.64	Iron-sulphur cluster biosynthesis family protein	[more]
AT1G10500.1	2.2e-10	29.58	chloroplast-localized ISCA-like protein	[more]
AT2G16710.2	3.0e-07	26.04	Iron-sulphur cluster biosynthesis family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035903	HesB-like domain superfamily	GENE3D	2.60.300.12		coord: 99..206 e-value: 8.2E-34 score: 118.0
IPR035903	HesB-like domain superfamily	SUPERFAMILY	89360	HesB-like domain	coord: 99..192
IPR016092	FeS cluster insertion protein	TIGRFAM	TIGR00049	TIGR00049	coord: 100..204 e-value: 1.4E-33 score: 113.2
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 100..199 e-value: 7.5E-15 score: 55.1
None	No IPR available	PANTHER	PTHR43011:SF4	BNACNNG10620D PROTEIN	coord: 55..206
None	No IPR available	PANTHER	PTHR43011	IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL	coord: 55..206

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG03G002740.2	ClCG03G002740.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0106035	protein maturation by [4Fe-4S] cluster transfer
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051537	2 iron, 2 sulfur cluster binding
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0051536	iron-sulfur cluster binding