ClCG02G016010 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG02G016010
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	Kunitz-type trypsin inhibitor
Location	CG_Chr02: 30406013 .. 30406934 (+)
RNA-Seq Expression	ClCG02G016010
Synteny	ClCG02G016010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTTCAGACAAAATTAAATCTCTCTTCTTGAAGAACTAAAAGATGATGATGTTCTATAAGTCACTTGCCATCTTTAGTTGCCTATGCTTCTTCATGCCCATATCTTCTACTGCCCAATCTGATGGTCCTCCCGTGCTCGACACCGAAGGCCGACCTCTACAGCGCGACGTTGAGTACTTCATCAAGCCTGCCATCACTGATGTCGCTGGTAATCTTACCCTAATTACCCGAAAAGGTGATCAGTGCCCGTTTTACGTTGGGCAACTACCACTTCTTTCACAAGATATAGGTATATATTAAACTATAATACTAAACTCAATTGATTCATAAGTTATATCATATTTAATATTTTATATGTATTTGATATAACGTAAGCTGATAGATTATGATACATTTAATTCTCAACATTAGGCTATGGAGTCACCTTCAGGCCTTATCTAGAGGGTGAAGATACAATTAGAGAAGGTAGAGATTTGAAGGTCGTGTTTCAAGCTTTTTCAATTTGCATAACGTCGACTGAATGGAAAGTGGGGGAGGCAGATCCTACTACCGGAAGGAGGTTTGTGAAGCTCGGGAGTGACAACACTGCCACCGGGTACTTTAGGATCGACAAGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTGTTGGCTTTGGATGGATCTGCATTTCCTTTTGAGTTTGTGAGAGTTGAGACAGCTGCAAAAGACCTTTGGTCAATCTTTCAACAGAAAAAATAAAGTGTGCAAATGTGTTCTAATTGCACTCATTTAAATTAAATAAAACCCCTGCAAGGATTTACTTTGTGTTCTTTAGTGTGTGTTATATTAATAATATGATTTGCC

mRNA sequence

ATTTTTCAGACAAAATTAAATCTCTCTTCTTGAAGAACTAAAAGATGATGATGTTCTATAAGTCACTTGCCATCTTTAGTTGCCTATGCTTCTTCATGCCCATATCTTCTACTGCCCAATCTGATGGTCCTCCCGTGCTCGACACCGAAGGCCGACCTCTACAGCGCGACGTTGAGTACTTCATCAAGCCTGCCATCACTGATGTCGCTGGTAATCTTACCCTAATTACCCGAAAAGGTGATCAGTGCCCGTTTTACGTTGGGCAACTACCACTTCTTTCACAAGATATAGGCTATGGAGTCACCTTCAGGCCTTATCTAGAGGGTGAAGATACAATTAGAGAAGGTAGAGATTTGAAGGTCGTGTTTCAAGCTTTTTCAATTTGCATAACGTCGACTGAATGGAAAGTGGGGGAGGCAGATCCTACTACCGGAAGGAGGTTTGTGAAGCTCGGGAGTGACAACACTGCCACCGGGTACTTTAGGATCGACAAGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTGTTGGCTTTGGATGGATCTGCATTTCCTTTTGAGTTTGTGAGAGTTGAGACAGCTGCAAAAGACCTTTGGTCAATCTTTCAACAGAAAAAATAAAGTGTGCAAATGTGTTCTAATTGCACTCATTTAAATTAAATAAAACCCCTGCAAGGATTTACTTTGTGTTCTTTAGTGTGTGTTATATTAATAATATGATTTGCC

Coding sequence (CDS)

ATGATGATGTTCTATAAGTCACTTGCCATCTTTAGTTGCCTATGCTTCTTCATGCCCATATCTTCTACTGCCCAATCTGATGGTCCTCCCGTGCTCGACACCGAAGGCCGACCTCTACAGCGCGACGTTGAGTACTTCATCAAGCCTGCCATCACTGATGTCGCTGGTAATCTTACCCTAATTACCCGAAAAGGTGATCAGTGCCCGTTTTACGTTGGGCAACTACCACTTCTTTCACAAGATATAGGCTATGGAGTCACCTTCAGGCCTTATCTAGAGGGTGAAGATACAATTAGAGAAGGTAGAGATTTGAAGGTCGTGTTTCAAGCTTTTTCAATTTGCATAACGTCGACTGAATGGAAAGTGGGGGAGGCAGATCCTACTACCGGAAGGAGGTTTGTGAAGCTCGGGAGTGACAACACTGCCACCGGGTACTTTAGGATCGACAAGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTGTTGGCTTTGGATGGATCTGCATTTCCTTTTGAGTTTGTGAGAGTTGAGACAGCTGCAAAAGACCTTTGGTCAATCTTTCAACAGAAAAAATAA

Protein sequence

MMMFYKSLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQKK

Homology

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BLAST of ClCG02G016010 vs. NCBI nr
Match: XP_038888505.1 (kunitz type trypsin inhibitor 111-like [Benincasa hispida])

HSP 1 Score: 409.5 bits (1051), Expect = 1.9e-110
Identity = 197/216 (91.20%), Postives = 205/216 (94.91%), Query Frame = 0


Query: 1   MMMFYKSLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 60
           MMMF+KSLAIFS LCFFM ISS AQ DGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL
Sbjct: 1   MMMFHKSLAIFSSLCFFMAISSIAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 60

Query: 61  ITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEW 120
           ITRKGDQCPFYVGQ+PLLSQDIG+GVTF PYL GEDTIREGRDLKVVFQAF+ICITST+W
Sbjct: 61  ITRKGDQCPFYVGQVPLLSQDIGFGVTFTPYLAGEDTIREGRDLKVVFQAFTICITSTQW 120

Query: 121 KVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILI 180
           KVGEADP TGRRFVKLG+D+TATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILI
Sbjct: 121 KVGEADPITGRRFVKLGNDDTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILI 180

Query: 181 EKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQKK 217
           EKGLRLLALDGSAFPFEFVRVE A K L SIFQ+KK
Sbjct: 181 EKGLRLLALDGSAFPFEFVRVEIAGKKLDSIFQEKK 216

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BLAST of ClCG02G016010 vs. NCBI nr
Match: XP_004139193.1 (endogenous alpha-amylase/subtilisin inhibitor [Cucumis sativus])

HSP 1 Score: 376.3 bits (965), Expect = 1.7e-100
Identity = 180/217 (82.95%), Postives = 198/217 (91.24%), Query Frame = 0


Query: 1   MMMFYKSL-AIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60
           MM F+KSL A+FS LCFFM I STAQ DGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTE 120
           LITRKGDQCPFYVGQ+PLLSQ+IG+GVT  PYLEG+DTIREGRDLK VFQAF+ICIT T+
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 121 WKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGIL 180
           WKVGE DP +GRRFVKLG+DNTATGYFRIDKS+LGVYNIGWCPSDVP +GRPRCGSAGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 181 IEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQKK 217
           IEKG+R LALDG AFPFEFVRV+T  ++L SI ++KK
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVDT-GEELGSILKEKK 216

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BLAST of ClCG02G016010 vs. NCBI nr
Match: KAA0063688.1 (endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa] >TYK18339.1 endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 368.2 bits (944), Expect = 4.7e-98
Identity = 180/218 (82.57%), Postives = 193/218 (88.53%), Query Frame = 0


Query: 1   MMMFYKSL-AIFSCLCFFMPISSTA-QSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60
           MM F+KSL AIFS LCFFM I STA Q DGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITST 120
           TLITRKGDQCPFYVGQ+PL SQ+ G+GV+  PY EGEDTIREGRDLK VFQ F+ICIT T
Sbjct: 61  TLITRKGDQCPFYVGQVPLFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 EWKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGI 180
           +WKVGEADP TGRRFVKLG DNTATGYFRIDKSDLGVYNIGWCPSDVPI+GRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LIEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQKK 217
           LIEKG+R LALDG AFPFEFVRV+   ++L SI Q+KK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVD-PVEELGSILQEKK 217

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BLAST of ClCG02G016010 vs. NCBI nr
Match: XP_008455014.1 (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo])

HSP 1 Score: 364.8 bits (935), Expect = 5.2e-97
Identity = 178/217 (82.03%), Postives = 192/217 (88.48%), Query Frame = 0


Query: 1   MMMFYKSL-AIFSCLCFFMPISSTA-QSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60
           MM F+KSL +IFS LCFFM I STA Q DGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITST 120
           TLITRKGDQCPFYVGQ+PLLSQ+ G+ V+  PY EGEDTIREGRDLK VFQ F+ICIT T
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 EWKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGI 180
           +WKVGEADP TGRRFVKLG DNTATGYFRIDKSDLGVYNIGWCPSDVPI+GRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LIEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQK 216
           LIEKG+R LALDG AFPFEFVRV+   ++L SI Q+K
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVD-PVEELGSILQEK 216

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BLAST of ClCG02G016010 vs. NCBI nr
Match: KAE8651587.1 (hypothetical protein Csa_023465 [Cucumis sativus])

HSP 1 Score: 357.5 bits (916), Expect = 8.4e-95
Identity = 167/199 (83.92%), Postives = 183/199 (91.96%), Query Frame = 0


Query: 18  MPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPL 77
           M I STAQ DGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQ+PL
Sbjct: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60

Query: 78  LSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLG 137
           LSQ+IG+GVT  PYLEG+DTIREGRDLK VFQAF+ICIT T+WKVGE DP +GRRFVKLG
Sbjct: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120

Query: 138 SDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIEKGLRLLALDGSAFPFE 197
           +DNTATGYFRIDKS+LGVYNIGWCPSDVP +GRPRCGSAGILIEKG+R LALDG AFPFE
Sbjct: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180

Query: 198 FVRVETAAKDLWSIFQQKK 217
           FVRV+T  ++L SI ++KK
Sbjct: 181 FVRVDT-GEELGSILKEKK 198

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BLAST of ClCG02G016010 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.6e-38
Identity = 83/175 (47.43%), Postives = 113/175 (64.57%), Query Frame = 0


Query: 31  VLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPLLSQDIGYGVTFRP 90
           V+DT G P++ D +YFI+PAIT   G+LTL+TR  + CPF VG  P   Q  G+ V   P
Sbjct: 27  VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTR--NSCPFNVGLDPDAPQ--GFAVLLSP 86

Query: 91  YLEG--EDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLGSDNTATG---- 150
           ++    ED +R GRDL+V+FQA + C  STEW++GE D TTGRRF+  G D++  G    
Sbjct: 87  FVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDATTGRRFIITGRDDSTVGSYGN 146

Query: 151 YFRIDKS-DLGVYNIGWCPSDVPIRGRPRCGSAGILIEKGLRLLALDGSAFPFEF 199
           +FRI ++   G++NI WCP++V    +  CG+ GI+ E G  LLALDGSA P  F
Sbjct: 147 FFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRENGKILLALDGSALPVAF 197

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BLAST of ClCG02G016010 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 2.3e-34
Identity = 82/205 (40.00%), Postives = 119/205 (58.05%), Query Frame = 0


Query: 1   MMMFYKSLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 60
           M +  ++L I + +C F+  ++ AQ     VLDT G P++ D EYFI+P IT+  G  T+
Sbjct: 3   MRLSIRTLIILAHVCLFITTTTIAQF----VLDTVGEPVEGDEEYFIRPVITNKGGRSTM 62

Query: 61  ITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGE--DTIREGRDLKVVFQAFSICITST 120
           ++R  + CP +VG L L     G  V F P+      D +R  RDL++ FQA S C+ ST
Sbjct: 63  VSR-NESCPLHVG-LELTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQST 122

Query: 121 EWKVGEADPTTGRRFVKLGSDNTATG----YFRIDKSDL-GVYNIGWCPSDVPIRGRPRC 180
           EW++GE D  +GRR +  G+D+   G    +FRI ++ L G+YNI WCP++V    +  C
Sbjct: 123 EWRLGEKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFEC 182

Query: 181 GSAGILIEKGLRLLALDGSAFPFEF 199
           G+  +L E G  LLALDG   P  F
Sbjct: 183 GTVDMLNENGKILLALDGGPLPLVF 201

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BLAST of ClCG02G016010 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.2e-32
Identity = 81/202 (40.10%), Postives = 114/202 (56.44%), Query Frame = 0


Query: 3   MFYKSLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLIT 62
           M  +SL IF     ++ +++T  S    V+DT G P++ D EYFI+PAIT   G  TL+T
Sbjct: 1   MSTRSLTIFILAHVWLLMATT--SIAQFVIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT 60

Query: 63  RKGDQCPFYVGQLPLLSQDIGYGVTFRPYL--EGEDTIREGRDLKVVFQAFSICITSTEW 122
             G  CP +VG L      +G  V F P+     +D +R  RDL+V F   + C  ST+W
Sbjct: 61  GNG-PCPLHVG-LDNTEGTLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDW 120

Query: 123 KVGEADPTTGRRFVKLGSDNTA---TGYFRIDKSDL-GVYNIGWCPSDVPIRGRPRCGSA 182
           ++GE D T+GRR +  G DN A     +FRI ++   G YNI WCP++     + +CG+ 
Sbjct: 121 RLGEKDATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTV 180

Query: 183 GILIEKGLRLLALDGSAFPFEF 199
           G++ E G  LLALDG A P  F
Sbjct: 181 GVIRENGKNLLALDGDALPVVF 198

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BLAST of ClCG02G016010 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 93.6 bits (231), Expect = 3.0e-18
Identity = 72/215 (33.49%), Postives = 101/215 (46.98%), Query Frame = 0


Query: 6   KSLAIFSCLCFFM---------PISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAG 65
           K L + S   FF+         P+ S A S   PVLD +G  L+    Y+I P + D  G
Sbjct: 2   KELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGG 61

Query: 66  NLTL--ITRKGD-QCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSI 125
            LT+   T  G   CP  V Q      D    + F P    ED +R   DL + F AF  
Sbjct: 62  GLTVSATTPNGTFVCPPRVVQ-TRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMP 121

Query: 126 C--ITSTEWKVGEADPTTGRRFVKLG------SDNTATGYFRIDK-SDLGVYNIGWCPSD 185
           C   +ST W++ + D +TG+ FV +G         T + +F+I++    G Y + +CP+ 
Sbjct: 122 CRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPT- 181

Query: 186 VPIRGRPRCGSAGILI-EKGLRLLALDGSAFPFEF 199
           V    + +CG  GI I +KG R LAL    F FEF
Sbjct: 182 VCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEF 214

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BLAST of ClCG02G016010 vs. ExPASy Swiss-Prot
Match: P07596 (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 82.4 bits (202), Expect = 6.8e-15
Identity = 47/141 (33.33%), Postives = 70/141 (49.65%), Query Frame = 0


Query: 17  FMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLP 76
           F P   +  +D PPV DT+G  L+ D  Y++  A     G LT+    G  CP +V Q P
Sbjct: 14  FWPAPPSRAADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDP 73

Query: 77  LLSQDIGYGVTFRPY--LEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFV 136
               D G+ V   PY     +  IR   D+++ F+A++ C+ STEW + +++   GRR V
Sbjct: 74  NGQHD-GFPVRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHI-DSELAAGRRHV 133

Query: 137 KLG-----SDNTATGYFRIDK 151
             G     S +     FRI+K
Sbjct: 134 ITGPVKDPSPSGRENAFRIEK 152

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BLAST of ClCG02G016010 vs. ExPASy TrEMBL
Match: A0A0A0LGB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 8.4e-101
Identity = 180/217 (82.95%), Postives = 198/217 (91.24%), Query Frame = 0


Query: 1   MMMFYKSL-AIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60
           MM F+KSL A+FS LCFFM I STAQ DGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTE 120
           LITRKGDQCPFYVGQ+PLLSQ+IG+GVT  PYLEG+DTIREGRDLK VFQAF+ICIT T+
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 121 WKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGIL 180
           WKVGE DP +GRRFVKLG+DNTATGYFRIDKS+LGVYNIGWCPSDVP +GRPRCGSAGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 181 IEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQKK 217
           IEKG+R LALDG AFPFEFVRV+T  ++L SI ++KK
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVDT-GEELGSILKEKK 216

[Close]

BLAST of ClCG02G016010 vs. ExPASy TrEMBL
Match: A0A5A7V7Y2 (Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00540 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.3e-98
Identity = 180/218 (82.57%), Postives = 193/218 (88.53%), Query Frame = 0


Query: 1   MMMFYKSL-AIFSCLCFFMPISSTA-QSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60
           MM F+KSL AIFS LCFFM I STA Q DGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITST 120
           TLITRKGDQCPFYVGQ+PL SQ+ G+GV+  PY EGEDTIREGRDLK VFQ F+ICIT T
Sbjct: 61  TLITRKGDQCPFYVGQVPLFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 EWKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGI 180
           +WKVGEADP TGRRFVKLG DNTATGYFRIDKSDLGVYNIGWCPSDVPI+GRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LIEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQKK 217
           LIEKG+R LALDG AFPFEFVRV+   ++L SI Q+KK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVD-PVEELGSILQEKK 217

[Close]

BLAST of ClCG02G016010 vs. ExPASy TrEMBL
Match: A0A1S3C0M7 (endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103495287 PE=3 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 2.5e-97
Identity = 178/217 (82.03%), Postives = 192/217 (88.48%), Query Frame = 0


Query: 1   MMMFYKSL-AIFSCLCFFMPISSTA-QSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60
           MM F+KSL +IFS LCFFM I STA Q DGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITST 120
           TLITRKGDQCPFYVGQ+PLLSQ+ G+ V+  PY EGEDTIREGRDLK VFQ F+ICIT T
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 EWKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGI 180
           +WKVGEADP TGRRFVKLG DNTATGYFRIDKSDLGVYNIGWCPSDVPI+GRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LIEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQK 216
           LIEKG+R LALDG AFPFEFVRV+   ++L SI Q+K
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVD-PVEELGSILQEK 216

[Close]

BLAST of ClCG02G016010 vs. ExPASy TrEMBL
Match: A0A6J1FD70 (alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111444311 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.5e-91
Identity = 164/199 (82.41%), Postives = 177/199 (88.94%), Query Frame = 0


Query: 2   MMFYKSLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLI 61
           MM  KSL  FS LC  M +SS AQS  PPVLDTEGRPLQ +VEYFIKPA+TDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLL 60

Query: 62  TRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWK 121
           TRKGDQCPF+VGQ+PL SQ+IG+GVTF PYL GEDTIREGRDLKVVFQAFSICITST W+
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWR 120

Query: 122 VGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIE 181
           VGE DPTTGRRFV+ G D+TATGYFRID+ + GVYNIGWCPSDVPIRGRPRCG AGILIE
Sbjct: 121 VGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIE 180

Query: 182 KGLRLLALDGSAFPFEFVR 201
           KGLRLLALDGSAFPFEFVR
Sbjct: 181 KGLRLLALDGSAFPFEFVR 197

[Close]

BLAST of ClCG02G016010 vs. ExPASy TrEMBL
Match: A0A6J1HLP6 (kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 7.4e-89
Identity = 162/199 (81.41%), Postives = 174/199 (87.44%), Query Frame = 0


Query: 2   MMFYKSLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLI 61
           MM  KSL  FS LC  M  SS AQS  PPVLD EGRPL+ +VEYFIKPAITDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAASSIAQS--PPVLDIEGRPLEINVEYFIKPAITDVAGNLTLL 60

Query: 62  TRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWK 121
           TRKGDQCPF+VGQ+PL SQ+IG+GVTF PYL  ED IREGRDLKVVFQAFSICITST W+
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWR 120

Query: 122 VGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIE 181
           VGEADPTTGRRFV+ G D+TATGYFRID+ + GVYNIGWCPSDVPIRGRPRCG AGILIE
Sbjct: 121 VGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIE 180

Query: 182 KGLRLLALDGSAFPFEFVR 201
           KGLRLLALDGSAFPFEFVR
Sbjct: 181 KGLRLLALDGSAFPFEFVR 197

[Close]

BLAST of ClCG02G016010 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 42.7 bits (99), Expect = 4.2e-04
Identity = 41/170 (24.12%), Postives = 69/170 (40.59%), Query Frame = 0


Query: 1   MMMFYKSLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 60
           + + + +L + S +  F   S+   +    VLD  G P+Q +V+Y+I PA     G L  
Sbjct: 4   LTLSFITLTVLSAI--FTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIP 63

Query: 61  ITRK---GDQC---PFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQA-FSI 120
             R     D C          P +S   G  VTF P       ++    L + F +   +
Sbjct: 64  SNRNLSTQDLCLNLDIVQSSSPFVS---GLPVTFSPLNTKVKHVQLSASLNLEFDSTVWL 123

Query: 121 CITSTEWKVGEADPTTGRRFVKLGSD-NTATGYFRIDKSDLGVYNIGWCP 163
           C  S  W++ +      + FV +G        +F+I + D   Y + +CP
Sbjct: 124 CPDSKVWRI-DHSVQLRKSFVSIGGQKGKGNSWFQI-QEDGDAYKLMYCP 166

The following BLAST results are available for this feature:

BLAST of ClCG02G016010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888505.1	1.9e-110	91.20	kunitz type trypsin inhibitor 111-like [Benincasa hispida]	[more]
XP_004139193.1	1.7e-100	82.95	endogenous alpha-amylase/subtilisin inhibitor [Cucumis sativus]	[more]
KAA0063688.1	4.7e-98	82.57	endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa] >T...	[more]
XP_008455014.1	5.2e-97	82.03	PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo]	[more]
KAE8651587.1	8.4e-95	83.92	hypothetical protein Csa_023465 [Cucumis sativus]	[more]

BLAST of ClCG02G016010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV7	2.6e-38	47.43	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
G7LCV1	2.3e-34	40.00	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
A0A072TH68	1.2e-32	40.10	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
P13087	3.0e-18	33.49	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P07596	6.8e-15	33.33	Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2	[more]

BLAST of ClCG02G016010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LGB4	8.4e-101	82.95	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1	[more]
A0A5A7V7Y2	2.3e-98	82.57	Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa O...	[more]
A0A1S3C0M7	2.5e-97	82.03	endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1FD70	3.5e-91	82.41	alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HLP6	7.4e-89	81.41	kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3...	[more]

BLAST of ClCG02G016010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73325.1	4.2e-04	24.12	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 30..202 e-value: 2.5E-29 score: 113.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 31..199 e-value: 1.6E-36 score: 125.9
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 6..201
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 31..47
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 30..200 e-value: 7.4378E-36 score: 122.451
None	No IPR available	GENE3D	2.80.10.50		coord: 28..206 e-value: 5.1E-39 score: 135.6
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 6..201
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 29..201

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG02G016010.1	ClCG02G016010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity