ClCG02G013715 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG02G013715
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionCN hydrolase domain-containing protein
LocationCG_Chr02: 27744026 .. 27748076 (-)
RNA-Seq ExpressionClCG02G013715
SyntenyClCG02G013715
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATATATGCATATGAAATTCTGTATGTTCCAATGTCAAGCGAAAAGTTTCAGTTCCATTTCGCTCCTTGAAATTTCATGGAAAAAATGTGGTTGCGATCAAACTGAGATGTCCGTTCCTCCGTGGCTATCTCCCAAACATAACCTGATTCAATTCACTACACCAACTGCATCAGCTCGAAATCACCTTCCCTTGATCCGTTCGAATTTCGCAAACGGAGGATTGGCGGACATGGCAGTTAACTCCGTTCGAGTCGCCGCCGCTCAAATGACCTCCATCAACGACCTCTCTTCCAACTTCGCCATCTGTTCTCGCCTCGTCAAAGTATGTTAACTCGCAGCATTCCTCATTGTTCGTGTCTTAATTATCATTTTATCTCTGCATTTGTCGATATCTACAAAGTCTTGAACATCTTTCATAATTGATCGATATATTATGAATCAAGACCGTTAACAGGCTAATTTCTGTTTGAAATGCTTGTTTGAATTTATGTTTGGGAGTAAATTATCGGTCCTCATGTAGAAAGAAAAGAATAACGAGTGCAAACAATCTGGTCACGAGTTTACCTCATCGCGATATTACTTTGAGTTGATGTTTTGCCGCTTTCCGCTGATCGGAGCTTTATTCTTCTTTTCTTCTTCTTCTTCCAGTTTCTTGAATTTCCTGTTAGGTGAATGCAATTTATCGATATGAATCTATGAGCTATCGATTAGAAAACAGTGGATTTGATGTCTGGCTGACTGATTTATCAATAAATTTATCGTTGTTCCTGTTTTGAAATGGTTTTGCTTTCAGCTAATCAAGGTAACATGATTACTTACATATTCATAGGAAGCAGTTTCAGCTGGAGCAAAATTGATATGCTTCCCAGAAGATTTTTCTTTTATGCCTGCCAAGGATGGGGAAAGTCAAAAGATTGCAGAGCCTTTAGACGGTCCAATTATGAATCAATACTGCTCTTTGGCAAGGTTGTTCGTAATTCTTAGGCTGTTACATCTCTGTCGAAGACACAAAAATGAATTTGTTACTCAAATTTTAATCTCTATCATCACAGTAATTTTAGTGTAACTTTTGTTTGAGTTGCTTCTCTGCAAACATACATAATTGATAATTCACATTGGTTTGAGTTCCCGTATATAGGCATAATCTACCGTAACAAATAGTGATTTCATGAACATCTTTAAAACGTCTTCTAATTTCTGTGTACTCTGAGTTAATTTGCAGAGAATCTAGCATATGGCTATCACTTGGAGGCTTTCAAGAAAAGGGACCCGATGATCAACATTTCTATAACACACATGTCATAGTTGACGACACTGGAACCATCAGGAGCAGTTATAGGAAAATACACCTGTAATACACTTTCTATTTATTTTATATTAATGCGTGTGCGTTTGTGAGAGAGAGGCAATGATCACATACTTTTGGGCACTTGATTGAATTTTACATTTGTAATAGTGAAAGTTACTTCATTTGTGTTGGAAGCTAATGTGACTGATAAGTACATTTCCTGCATTATTTTGGATGCTTCGTCTTTATGCTGTGCAATTATTTTATGTAAAGGTTTGATGTCGACATTCCTGGTGGAAGAGTATATAAAGAAAGCAGCTATACAAAGGCAGGTAACCTTAAAATCTTATGAATGCAGAAATATGCATAATGGTTCTCTGTCATTTTTCCTTTCTTCATTATGTTAAGAGACTACCCTTAGCGTGGCTACAATGTCAAAGTAATTTGCGGTGAGGCAATGTGGCATGAGTCAATTATATTCAATGATTTGGAAAAAATTTGTGCATCTTAATAAGTTAGTTCACATTTTAGGAGAAGACATTGTAGCAGTTGACAGTCCTATTGGACGACTTGGTCTGACTGTATGCTATGATTTGAGATTTCCAGAGCTCTGCCAGCAGTTGAGATTCCAACACAACGCTCAGGTATGAATGATTCAAGTGTTTATTGATATAATATTTTCCTGGCTGCTAACGTTTGCTTGAGTTTGGATAAAAACCGGCAGGTTTTATTGGTTCCTTCAGCTTTTACAAAAGAAACTGGGGAGGCACATTGGGAAATTCTTCTTCGTTCTCGTGCAATTGAGAATCAATGCTATGTAAGAATTCCTTTCAACACACTAGCATACCAGCTTCTCGTTTTGTTTAACAAATGTTGGGGGAAAATAACTGTTAGACCCCCGATTGTTCACACCTATTATAGGAGGGGTAAAAAGGGAATTAACCATGAGATTATGGGGTGCAAAGTTGGGGTGGACGTGGCTGCCAATTCGGTTGGAAAGGAGGGGACCAGTGGGGGCAAAGATTGAGGGGTTTATGAAGGGGTTTGGGAGAGCCATGGAGGGTATGAAAAACATGAAGGCTAACTGAAGCTTGGGGAGGGAGGCACTCTCGAAATTGCCCAATTTCTTCCTATATCTTTTCTCTTTGTTCTTATCTTGTCATTGTCGTTTTAATGTTTGTTGCTGCTGGAATTTCTTGTGATGAACCATATTGTGGATAAATAAATCAGTAGCTTGTGGCTGGATGATTTCTTGTTGTGTTTGGATTAGATAATGGGCTGGATATTGGTTTGGTGTGTGTTTGTTAGATAATCTTGAGTGCAGGTATCTAACAAATTGGTATTAGAGCAAGTTTTCATCCTGGTGAGAACAAGATAAATGGCGATGAAGGAAACAGAGGAAAAATTGGAAACACACGATCGTGATATTTCTGGGCTGAAGGAATTGGTCTTCGCAATGTGTAAGAACGTCGATCAGTTGATGGGGGAGATGAGGGAAATCACCTTGAGCAGAGGGAAGGACGGTTCCCTGGTAACGGAGGGGTCTCTGAAACGCAGAGCAGGAACAGAGGTGGAGGAAGGCGAATCGTCTCAAGCAAAAGGAGGAGGGGATAGAGGCAAATATAAGAGGCTAGAGATGTCTATATTCAAGGGAGATCACCCTGACTCGTGGGTATATCGCGCTGAACACTACTTCGAGATTCATGAGCTGACAAATGAGGAGAAAATTAAGGTGGCGATCATCAGCTTCGATCACGATTCGGTGGATTGGTATCGCTGGAGTAACAATCGGAAACCAATTTGGACATGGGAGGAACTAAAGAAGGGATCGATTTCTAGTCGCATAGCTTCATAATAATTCTGTAAAGCTTCCACATAATTTCCTTTGGATTGAGCCGACATCCGTTACGGTCGTTCGTTATTCCATTCAAAGAATCTCCGCTCCAGAACTGTATGTGAGATTTTCATCTCATATGGCTCCCCCTTTATGTGATGCGCATAATGAGATGAGAATAATACATGAAATCAAAAAAGATTGAAATACTCTCATTATGAACTCATGGGACTAGCGTTTTTACAAAAAATCTCTAGCCAACCTTCCTGTAAAAGATCTTTTCTTAACATCAAGCATGGTGTTACTAGATAAAAAAAAGGGTAACTCTAACAATTTCTTTGTCCTCAATGCCTCTAAATTTCCAGGAATTAGTCACTTCAATAGTCTTCGATGGTTCTACAGGAAGCCTGACGAGACGATTTCTAGCGATCAAATAGGAAGGGAGCTACGCTGAATATCGAAAACAGTTTGAGAGGTTTTTCGCGCCGGTATCGGAGATATCCGAGTCAGTGTTGGAAGGAACCTTTATCAACGAATTGGATCCGGAGTTGAAAGCCAAGGTGGAGAGTCGGAATCCAGTAGACTTAGAGGAAGCTATGAGAGAGGCGCAGGCCATCGACGATGAGCTTCAGGCGATCAAGGAAATCAAGGGTTCCGTGTGGACGAAGGGGGAGGGAAAGGTCCAAGAGTCTAATAAAATGGGCCAAGGTGCGGACTACCGGCCCAATGCCGCAAAATCCAAATACCCGAAAAATTCGAGGCTCCGAGAAGAGAATGGGCGACCCGATGGCTTTCTGATGCAGAAATCTGAGAGAAGCGGGAAAAGGGGTTGTGTTTCAAATGCGACGAGAAGTACTCCCCCGACCACTGCTGTAAGACTCGAGAGAAGAGAGAACTCTGCTTATTTATCGCCGGCGAAGGCCAAGTGA

mRNA sequence

ATATATGCATATGAAATTCTGTATGTTCCAATGTCAAGCGAAAAGTTTCAGTTCCATTTCGCTCCTTGAAATTTCATGGAAAAAATGTGGTTGCGATCAAACTGAGATGTCCGTTCCTCCGTGGCTATCTCCCAAACATAACCTGATTCAATTCACTACACCAACTGCATCAGCTCGAAATCACCTTCCCTTGATCCGTTCGAATTTCGCAAACGGAGGATTGGCGGACATGGCAGTTAACTCCGTTCGAGTCGCCGCCGCTCAAATGACCTCCATCAACGACCTCTCTTCCAACTTCGCCATCTGTTCTCGCCTCGTCAAAGAAGCAGTTTCAGCTGGAGCAAAATTGATATGCTTCCCAGAAGATTTTTCTTTTATGCCTGCCAAGGATGGGGAAAGTCAAAAGATTGCAGAGCCTTTAGACGGTCCAATTATGAATCAATACTGCTCTTTGGCAAGAGAATCTAGCATATGGCTATCACTTGGAGGCTTTCAAGAAAAGGGACCCGATGATCAACATTTCTATAACACACATGTCATAGTTGACGACACTGGAACCATCAGGAGCAGTTATAGGAAAATACACCTGTTTGATGTCGACATTCCTGGTGGAAGAGTATATAAAGAAAGCAGCTATACAAAGGCAGGAGAAGACATTGTAGCAGTTGACAGTCCTATTGGACGACTTGGTCTGACTGTATGCTATGATTTGAGATTTCCAGAGCTCTGCCAGCAGTTGAGATTCCAACACAACGCTCAGGTTTTATTGGTTCCTTCAGCTTTTACAAAAGAAACTGGGGAGGCACATTGGGAAATTCTTCTTCGTTCTCGTGCAATTGAGAATCAATGCTATTTTGAGAGGTTTTTCGCGCCGGTATCGGAGATATCCGAGTCAGTGTTGGAAGGAACCTTTATCAACGAATTGGATCCGGAGTTGAAAGCCAAGGTGGAGAGTCGGAATCCAGTAGACTTAGAGGAAGCTATGAGAGAGGCGCAGGCCATCGACGATGAGCTTCAGGCGATCAAGGAAATCAAGGGTTCCGTGTGGACGAAGGGGGAGGGAAAGGTCCAAGAGTCTAATAAAATGGGCCAAGGTGCGGACTACCGGCCCAATGCCGCAAAATCCAAATACCCGAAAAATTCGAGGCTCCGAGAAGAGAATGGGCGACCCGATGGCTTTCTGATGCAGAAATCTGAGAGAAGCGGGAAAAGGGGTTGTGTTTCAAATGCGACGAGAAGTACTCCCCCGACCACTGCTGTAAGACTCGAGAGAAGAGAGAACTCTGCTTATTTATCGCCGGCGAAGGCCAAGTGA

Coding sequence (CDS)

ATGCATATGAAATTCTGTATGTTCCAATGTCAAGCGAAAAGTTTCAGTTCCATTTCGCTCCTTGAAATTTCATGGAAAAAATGTGGTTGCGATCAAACTGAGATGTCCGTTCCTCCGTGGCTATCTCCCAAACATAACCTGATTCAATTCACTACACCAACTGCATCAGCTCGAAATCACCTTCCCTTGATCCGTTCGAATTTCGCAAACGGAGGATTGGCGGACATGGCAGTTAACTCCGTTCGAGTCGCCGCCGCTCAAATGACCTCCATCAACGACCTCTCTTCCAACTTCGCCATCTGTTCTCGCCTCGTCAAAGAAGCAGTTTCAGCTGGAGCAAAATTGATATGCTTCCCAGAAGATTTTTCTTTTATGCCTGCCAAGGATGGGGAAAGTCAAAAGATTGCAGAGCCTTTAGACGGTCCAATTATGAATCAATACTGCTCTTTGGCAAGAGAATCTAGCATATGGCTATCACTTGGAGGCTTTCAAGAAAAGGGACCCGATGATCAACATTTCTATAACACACATGTCATAGTTGACGACACTGGAACCATCAGGAGCAGTTATAGGAAAATACACCTGTTTGATGTCGACATTCCTGGTGGAAGAGTATATAAAGAAAGCAGCTATACAAAGGCAGGAGAAGACATTGTAGCAGTTGACAGTCCTATTGGACGACTTGGTCTGACTGTATGCTATGATTTGAGATTTCCAGAGCTCTGCCAGCAGTTGAGATTCCAACACAACGCTCAGGTTTTATTGGTTCCTTCAGCTTTTACAAAAGAAACTGGGGAGGCACATTGGGAAATTCTTCTTCGTTCTCGTGCAATTGAGAATCAATGCTATTTTGAGAGGTTTTTCGCGCCGGTATCGGAGATATCCGAGTCAGTGTTGGAAGGAACCTTTATCAACGAATTGGATCCGGAGTTGAAAGCCAAGGTGGAGAGTCGGAATCCAGTAGACTTAGAGGAAGCTATGAGAGAGGCGCAGGCCATCGACGATGAGCTTCAGGCGATCAAGGAAATCAAGGGTTCCGTGTGGACGAAGGGGGAGGGAAAGGTCCAAGAGTCTAATAAAATGGGCCAAGGTGCGGACTACCGGCCCAATGCCGCAAAATCCAAATACCCGAAAAATTCGAGGCTCCGAGAAGAGAATGGGCGACCCGATGGCTTTCTGATGCAGAAATCTGAGAGAAGCGGGAAAAGGGGTTGTGTTTCAAATGCGACGAGAAGTACTCCCCCGACCACTGCTGTAAGACTCGAGAGAAGAGAGAACTCTGCTTATTTATCGCCGGCGAAGGCCAAGTGA

Protein sequence

MHMKFCMFQCQAKSFSSISLLEISWKKCGCDQTEMSVPPWLSPKHNLIQFTTPTASARNHLPLIRSNFANGGLADMAVNSVRVAAAQMTSINDLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGPIMNQYCSLARESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVDIPGGRVYKESSYTKAGEDIVAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPSAFTKETGEAHWEILLRSRAIENQCYFERFFAPVSEISESVLEGTFINELDPELKAKVESRNPVDLEEAMREAQAIDDELQAIKEIKGSVWTKGEGKVQESNKMGQGADYRPNAAKSKYPKNSRLREENGRPDGFLMQKSERSGKRGCVSNATRSTPPTTAVRLERRENSAYLSPAKAK
Homology
BLAST of ClCG02G013715 vs. NCBI nr
Match: XP_038893375.1 (deaminated glutathione amidase, chloroplastic/cytosolic [Benincasa hispida])

HSP 1 Score: 481.1 bits (1237), Expect = 1.0e-131
Identity = 247/312 (79.17%), Postives = 260/312 (83.33%), Query Frame = 0

Query: 35  MSVPPWLSPKHNLIQFTTPTASARNHLPLIRSNFAN---GGLADMAVNSVRVAAAQMTSI 94
           MSV PWLSPK NL++FTTPTASAR+HLPLIRSNFAN   GG ADMAVNSVRVA AQMTSI
Sbjct: 1   MSVSPWLSPKQNLVRFTTPTASARDHLPLIRSNFANAFAGGPADMAVNSVRVAVAQMTSI 60

Query: 95  NDLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGPIMNQYCSLA 154
           N LSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPA DGESQKIAEPLDGPIMNQYCSLA
Sbjct: 61  NGLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPANDGESQKIAEPLDGPIMNQYCSLA 120

Query: 155 RESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVDIPGGRVYKESSY 214
           RESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVD+PGGRVYKESSY
Sbjct: 121 RESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVDVPGGRVYKESSY 180

Query: 215 TKAGEDIVAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPSAFTKETGEAHWEI 274
           TKAGEDIVAVDSPIGRLGLTVCYDLRFPEL Q LRFQHNAQVLLVPSAFTKETGEAHWEI
Sbjct: 181 TKAGEDIVAVDSPIGRLGLTVCYDLRFPELYQHLRFQHNAQVLLVPSAFTKETGEAHWEI 240

Query: 275 LLRSRAIENQCY----------------------FERFFAPVSEISESVLEGTFINELDP 322
           LLRSRAIENQCY                       + +   V  +S+ +  G  I ++D 
Sbjct: 241 LLRSRAIENQCYVIAAAQAGKANEKRESYGDSLIIDPWGKIVGRLSDRLATGIAIADIDF 300

BLAST of ClCG02G013715 vs. NCBI nr
Match: XP_008445813.1 (PREDICTED: nitrilase-like protein 2 [Cucumis melo] >XP_008445815.1 PREDICTED: nitrilase-like protein 2 [Cucumis melo] >XP_008445816.1 PREDICTED: nitrilase-like protein 2 [Cucumis melo])

HSP 1 Score: 464.2 bits (1193), Expect = 1.3e-126
Identity = 229/252 (90.87%), Postives = 235/252 (93.25%), Query Frame = 0

Query: 35  MSVPPWLSPKHNLIQFTTPTASARNHLPLIRSNFAN---GGLADMAVNSVRVAAAQMTSI 94
           MSV PWLSPK NLI+F TPTASAR HLPLIRSNFAN   GG ADMAVNSVRVA AQMTS+
Sbjct: 1   MSVSPWLSPKQNLIRFLTPTASARYHLPLIRSNFANAFAGGSADMAVNSVRVAVAQMTSV 60

Query: 95  NDLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGPIMNQYCSLA 154
           N+LSSNFA CSRLVKEA SAGAKLICFPEDFSFMPA +GESQKIAEPLDGPIMNQYCSLA
Sbjct: 61  NNLSSNFATCSRLVKEAASAGAKLICFPEDFSFMPASEGESQKIAEPLDGPIMNQYCSLA 120

Query: 155 RESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVDIPGGRVYKESSY 214
           RES IWLSLGGFQEKGPDDQHFYNTHVIVDDTGTI SSYRKIHLFDVD+PGGRVYKESSY
Sbjct: 121 RESRIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTITSSYRKIHLFDVDVPGGRVYKESSY 180

Query: 215 TKAGEDIVAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPSAFTKETGEAHWEI 274
           TKAGE IVAVDSPIGRLGLTVCYDLRFPEL  QLRFQHNA+VLLVPSAFTKETGEAHWEI
Sbjct: 181 TKAGEHIVAVDSPIGRLGLTVCYDLRFPELYLQLRFQHNAKVLLVPSAFTKETGEAHWEI 240

Query: 275 LLRSRAIENQCY 284
           LLRSRAIENQCY
Sbjct: 241 LLRSRAIENQCY 252

BLAST of ClCG02G013715 vs. NCBI nr
Match: KAA0034030.1 (nitrilase-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 464.2 bits (1193), Expect = 1.3e-126
Identity = 229/252 (90.87%), Postives = 235/252 (93.25%), Query Frame = 0

Query: 35  MSVPPWLSPKHNLIQFTTPTASARNHLPLIRSNFAN---GGLADMAVNSVRVAAAQMTSI 94
           MSV PWLSPK NLI+F TPTASAR HLPLIRSNFAN   GG ADMAVNSVRVA AQMTS+
Sbjct: 1   MSVSPWLSPKQNLIRFLTPTASARYHLPLIRSNFANAFAGGSADMAVNSVRVAVAQMTSV 60

Query: 95  NDLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGPIMNQYCSLA 154
           N+LSSNFA CSRLVKEA SAGAKLICFPEDFSFMPA +GESQKIAEPLDGPIMNQYCSLA
Sbjct: 61  NNLSSNFATCSRLVKEAASAGAKLICFPEDFSFMPASEGESQKIAEPLDGPIMNQYCSLA 120

Query: 155 RESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVDIPGGRVYKESSY 214
           RES IWLSLGGFQEKGPDDQHFYNTHVIVDDTGTI SSYRKIHLFDVD+PGGRVYKESSY
Sbjct: 121 RESRIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTITSSYRKIHLFDVDVPGGRVYKESSY 180

Query: 215 TKAGEDIVAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPSAFTKETGEAHWEI 274
           TKAGE IVAVDSPIGRLGLTVCYDLRFPEL  QLRFQHNA+VLLVPSAFTKETGEAHWEI
Sbjct: 181 TKAGEHIVAVDSPIGRLGLTVCYDLRFPELYLQLRFQHNAKVLLVPSAFTKETGEAHWEI 240

Query: 275 LLRSRAIENQCY 284
           LLRSRAIENQCY
Sbjct: 241 LLRSRAIENQCY 252

BLAST of ClCG02G013715 vs. NCBI nr
Match: XP_023525991.1 (nitrilase-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 463.0 bits (1190), Expect = 2.9e-126
Identity = 235/311 (75.56%), Postives = 254/311 (81.67%), Query Frame = 0

Query: 35  MSVPPWLSPKHNLIQFTTPTASARNHLPLIRSNFANG--GLADMAVNSVRVAAAQMTSIN 94
           MSV PWLSP   LI+FT PT SAR+HLPLIRSNFA    G +DMAVNSVRVA AQMTSIN
Sbjct: 1   MSVSPWLSPNQTLIRFTIPTVSARDHLPLIRSNFATAFTGGSDMAVNSVRVAVAQMTSIN 60

Query: 95  DLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGPIMNQYCSLAR 154
           DLS NFA CSRLVKEAVSAGAKL+CFPEDFSFMPAKDGE QKIAEPLDGPI+NQYCSLAR
Sbjct: 61  DLSFNFATCSRLVKEAVSAGAKLVCFPEDFSFMPAKDGEGQKIAEPLDGPILNQYCSLAR 120

Query: 155 ESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVDIPGGRVYKESSYT 214
           ESSIWLSLGGFQEKGPDD+HFYNTHVIVDD+GTIRS+YRKIHLFDVD+PGGRVYKESSYT
Sbjct: 121 ESSIWLSLGGFQEKGPDDEHFYNTHVIVDDSGTIRSTYRKIHLFDVDVPGGRVYKESSYT 180

Query: 215 KAGEDIVAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPSAFTKETGEAHWEIL 274
           KAGEDIVAVDSPIGRLGLTVCYDLRFPEL QQLRFQHNAQVLLVPSAFTKETGEAHWEIL
Sbjct: 181 KAGEDIVAVDSPIGRLGLTVCYDLRFPELYQQLRFQHNAQVLLVPSAFTKETGEAHWEIL 240

Query: 275 LRSRAIENQCY----------------------FERFFAPVSEISESVLEGTFINELDPE 322
           LRSRAIENQCY                       + +   V  +S+ +  G  + ++D +
Sbjct: 241 LRSRAIENQCYVIAAAQAGKAIGKRESYGDSLIIDPWGKIVGRLSDRLATGIAVADIDFD 300

BLAST of ClCG02G013715 vs. NCBI nr
Match: XP_022956809.1 (nitrilase-like protein 2 [Cucurbita moschata])

HSP 1 Score: 463.0 bits (1190), Expect = 2.9e-126
Identity = 235/311 (75.56%), Postives = 254/311 (81.67%), Query Frame = 0

Query: 35  MSVPPWLSPKHNLIQFTTPTASARNHLPLIRSNFANG--GLADMAVNSVRVAAAQMTSIN 94
           MSV PWLSP   LI+FT PT SAR+HLPLIRSNFA    G +DMAVNSVRVA AQMTSIN
Sbjct: 1   MSVSPWLSPNQTLIRFTIPTVSARDHLPLIRSNFATAFTGGSDMAVNSVRVAVAQMTSIN 60

Query: 95  DLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGPIMNQYCSLAR 154
           DLS NFA CSRLVKEAVSAGAKL+CFPEDFSFMPAKDGE QKIAEPLDGPIMNQYCSLAR
Sbjct: 61  DLSFNFATCSRLVKEAVSAGAKLVCFPEDFSFMPAKDGEGQKIAEPLDGPIMNQYCSLAR 120

Query: 155 ESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVDIPGGRVYKESSYT 214
           ESSIWLSLGGFQEKGPDD+HFYNTHVIVDD+GTIRS+YRKIHLFDVD+PGGRVYKESSYT
Sbjct: 121 ESSIWLSLGGFQEKGPDDEHFYNTHVIVDDSGTIRSTYRKIHLFDVDVPGGRVYKESSYT 180

Query: 215 KAGEDIVAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPSAFTKETGEAHWEIL 274
           KAGEDIVAVDSPIGRLGLTVCYDLRFPEL QQLRFQHN+QVLLVPSAFTKETGEAHWEIL
Sbjct: 181 KAGEDIVAVDSPIGRLGLTVCYDLRFPELYQQLRFQHNSQVLLVPSAFTKETGEAHWEIL 240

Query: 275 LRSRAIENQCY----------------------FERFFAPVSEISESVLEGTFINELDPE 322
           LRSRAIENQCY                       + +   V  +S+ +  G  + ++D +
Sbjct: 241 LRSRAIENQCYVIAAAQAGKANGKRESYGDSLIIDPWGKIVGRLSDRLATGITVADIDFD 300

BLAST of ClCG02G013715 vs. ExPASy Swiss-Prot
Match: Q94JV5 (Deaminated glutathione amidase, chloroplastic/cytosolic OS=Arabidopsis thaliana OX=3702 GN=NLP2 PE=1 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.3e-86
Identity = 167/301 (55.48%), Postives = 207/301 (68.77%), Query Frame = 0

Query: 48  IQFTTPTASAR----NHLPLIRSNFANGGLADMAVNSVRVAAAQMTSINDLSSNFAICSR 107
           + FT P+   R    + +PL  +   N         +VRVAAAQMTS+NDL +NFA CSR
Sbjct: 8   LDFTKPSLFTRITLSSQIPLTMATTVN--------KTVRVAAAQMTSVNDLMTNFATCSR 67

Query: 108 LVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGPIMNQYCSLARESSIWLSLGGF 167
           LV+EA  AGAKLICFPE+FSF+  K+GES KIAEPLDGP+M +YCSLAR+S+IWLSLGGF
Sbjct: 68  LVQEAALAGAKLICFPENFSFVGDKEGESVKIAEPLDGPVMERYCSLARDSNIWLSLGGF 127

Query: 168 QEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVDIPGGRVYKESSYTKAGEDIVAVDS 227
           QE+  DD H  NTHV++DD G IR +Y+K+HLFDVD+PGG  YKESS+T  G  IV+VDS
Sbjct: 128 QERF-DDTHLCNTHVVIDDAGMIRDTYQKMHLFDVDVPGGSSYKESSFTVPGTKIVSVDS 187

Query: 228 PIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPSAFTKETGEAHWEILLRSRAIENQCY 287
           P+GRLGLTVCYDLRFP++ QQLRF+  AQVLLVPSAFTK TGEAHWEILLR+RAIE QCY
Sbjct: 188 PVGRLGLTVCYDLRFPKIYQQLRFEQKAQVLLVPSAFTKVTGEAHWEILLRARAIETQCY 247

Query: 288 ----------------------FERFFAPVSEISESVLEGTFINELDPELKAKVESRNPV 323
                                  + +   V  + + V  G  + ++D  L   V ++ P+
Sbjct: 248 VIAAAQAGKHNEKRESYGDTLIIDPWGTVVGRLPDRVSTGIVVADIDFSLIDSVRTKMPI 299

BLAST of ClCG02G013715 vs. ExPASy Swiss-Prot
Match: Q557J5 (Deaminated glutathione amidase OS=Dictyostelium discoideum OX=44689 GN=nit1-1 PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.8e-51
Identity = 108/209 (51.67%), Postives = 139/209 (66.51%), Query Frame = 0

Query: 82  RVAAAQMTSINDLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPA--KDGESQKIAEPL 141
           R+   Q+TS N+   NF  C  ++++AV     L C PE F+F+       ES+  AE L
Sbjct: 14  RIGLGQITSTNNKEDNFRKCKEMIEKAVENKVNLFCLPECFAFISGGIHQFESRDNAEYL 73

Query: 142 D--GPIMNQYCSLARESSIWLSLGGFQEKGPDDQH--FYNTHVIVDDTGTIRSSYRKIHL 201
           D  G I+ +Y  LA++++IWLSLGGF EK  DD +   YNTH+I+D  G I   YRK+HL
Sbjct: 74  DQKGGIIERYKDLAKQNNIWLSLGGFHEKILDDPNDMIYNTHLIIDSNGVIVCEYRKMHL 133

Query: 202 FDVDIPG-GRVYKESSYTKAGEDIVAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVL 261
           FDVDIP  G    ES   K G D+V  DSP+G+LGL++CYDLRFPEL   LR + +AQ+L
Sbjct: 134 FDVDIPSKGVKMNESKVVKGGNDLVVCDSPVGKLGLSICYDLRFPELYLSLR-RMDAQIL 193

Query: 262 LVPSAFTKETGEAHWEILLRSRAIENQCY 284
           LVPSAF K TGEAHW+ LL++RAIENQ Y
Sbjct: 194 LVPSAFMKSTGEAHWKPLLQARAIENQTY 221

BLAST of ClCG02G013715 vs. ExPASy Swiss-Prot
Match: Q8VDK1 (Deaminated glutathione amidase OS=Mus musculus OX=10090 GN=Nit1 PE=1 SV=2)

HSP 1 Score: 201.8 bits (512), Expect = 1.6e-50
Identity = 101/205 (49.27%), Postives = 135/205 (65.85%), Query Frame = 0

Query: 83  VAAAQMTSINDLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGP 142
           VA  Q+TS  +   NF  C+ LV+EA   GA L   PE F F+     E+  ++EPL+G 
Sbjct: 45  VAVCQVTSTPNKQENFKTCAELVQEAARLGACLAFLPEAFDFIARNPAETLLLSEPLNGD 104

Query: 143 IMNQYCSLARESSIWLSLGGFQEKGPD---DQHFYNTHVIVDDTGTIRSSYRKIHLFDVD 202
           ++ QY  LARE  IWLSLGGF E+G D   +Q  YN HV+++  G++ +SYRK HL DV+
Sbjct: 105 LLGQYSQLARECGIWLSLGGFHERGQDWEQNQKIYNCHVLLNSKGSVVASYRKTHLCDVE 164

Query: 203 IPGGRVYKESSYTKAGEDI-VAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPS 262
           IPG    +ES+YTK G  +   V +P G++GL +CYD+RFPEL  +L  Q  A++L  PS
Sbjct: 165 IPGQGPMRESNYTKPGGTLEPPVKTPAGKVGLAICYDMRFPELSLKLA-QAGAEILTYPS 224

Query: 263 AFTKETGEAHWEILLRSRAIENQCY 284
           AF   TG AHWE+LLR+RAIE+QCY
Sbjct: 225 AFGSVTGPAHWEVLLRARAIESQCY 248

BLAST of ClCG02G013715 vs. ExPASy Swiss-Prot
Match: Q7TQ94 (Deaminated glutathione amidase OS=Rattus norvegicus OX=10116 GN=Nit1 PE=2 SV=2)

HSP 1 Score: 200.3 bits (508), Expect = 4.5e-50
Identity = 101/205 (49.27%), Postives = 133/205 (64.88%), Query Frame = 0

Query: 83  VAAAQMTSINDLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGP 142
           VA  Q+TS  +   NF  C+ LV+EA   GA L   PE F F+     E+  ++EPLDG 
Sbjct: 50  VAVCQVTSTPNKQENFKTCAELVQEATRLGACLAFLPEAFDFIARNPAETLLLSEPLDGD 109

Query: 143 IMNQYCSLARESSIWLSLGGFQEKGPD---DQHFYNTHVIVDDTGTIRSSYRKIHLFDVD 202
           ++ QY  LARE  IWLSLGGF E+G D    Q  YN HV+++  G++ +SYRK HL DV+
Sbjct: 110 LLGQYSQLARECGIWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGSVVASYRKTHLCDVE 169

Query: 203 IPGGRVYKESSYTKAGEDI-VAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPS 262
           IPG    +ES+YT  G  +   V +P G++GL +CYD+RFPEL  +L  Q  A++L  PS
Sbjct: 170 IPGQGPMRESNYTMPGYALEPPVKTPAGKVGLAICYDMRFPELSLKLA-QAGAEILTYPS 229

Query: 263 AFTKETGEAHWEILLRSRAIENQCY 284
           AF   TG AHWE+LLR+RAIE+QCY
Sbjct: 230 AFGSVTGPAHWEVLLRARAIESQCY 253

BLAST of ClCG02G013715 vs. ExPASy Swiss-Prot
Match: Q32LH4 (Deaminated glutathione amidase OS=Bos taurus OX=9913 GN=NIT1 PE=2 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.2e-49
Identity = 104/253 (41.11%), Postives = 151/253 (59.68%), Query Frame = 0

Query: 41  LSPKHNLIQFTT------PTASARNHLPLIRSNFANGGLADMAVNSVRVAAAQMTSINDL 100
           + P H L+   +      P  S     P +R+  A+   +++ +    VA  Q+TS  D 
Sbjct: 6   IRPPHQLLSLLSCPGLRIPRFSVLCAPPRLRTMAASSSFSELPL----VAVCQVTSTPDK 65

Query: 101 SSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGPIMNQYCSLARES 160
             NF  C+ L++EA   GA L   PE F F+     E+++++EPL G ++ +Y  LARE 
Sbjct: 66  EQNFKTCAELIREAARLGACLAFLPEAFDFIARDPEETRRLSEPLSGNLLEEYTQLAREC 125

Query: 161 SIWLSLGGFQEKGPD---DQHFYNTHVIVDDTGTIRSSYRKIHLFDVDIPGGRVYKESSY 220
            +WLSLGGF E+G D    Q  YN HVI+++ G++ ++YRK HL DV+IPG    +ES+ 
Sbjct: 126 GLWLSLGGFHERGQDWEQTQKIYNCHVIMNNMGSVVATYRKTHLCDVEIPGQGPMRESNS 185

Query: 221 TKAGEDIVA-VDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPSAFTKETGEAHWE 280
           T  G  + + + +P G++GL +CYD+RFPEL   L  Q  A++L  PSAF   TG AHWE
Sbjct: 186 TIPGPSLESPISTPAGKIGLAICYDMRFPELSLAL-VQAGAEILTYPSAFGSVTGPAHWE 245

Query: 281 ILLRSRAIENQCY 284
           +LLR+RAIE QCY
Sbjct: 246 VLLRARAIETQCY 253

BLAST of ClCG02G013715 vs. ExPASy TrEMBL
Match: A0A1S3BDK3 (nitrilase-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103488728 PE=4 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 6.2e-127
Identity = 229/252 (90.87%), Postives = 235/252 (93.25%), Query Frame = 0

Query: 35  MSVPPWLSPKHNLIQFTTPTASARNHLPLIRSNFAN---GGLADMAVNSVRVAAAQMTSI 94
           MSV PWLSPK NLI+F TPTASAR HLPLIRSNFAN   GG ADMAVNSVRVA AQMTS+
Sbjct: 1   MSVSPWLSPKQNLIRFLTPTASARYHLPLIRSNFANAFAGGSADMAVNSVRVAVAQMTSV 60

Query: 95  NDLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGPIMNQYCSLA 154
           N+LSSNFA CSRLVKEA SAGAKLICFPEDFSFMPA +GESQKIAEPLDGPIMNQYCSLA
Sbjct: 61  NNLSSNFATCSRLVKEAASAGAKLICFPEDFSFMPASEGESQKIAEPLDGPIMNQYCSLA 120

Query: 155 RESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVDIPGGRVYKESSY 214
           RES IWLSLGGFQEKGPDDQHFYNTHVIVDDTGTI SSYRKIHLFDVD+PGGRVYKESSY
Sbjct: 121 RESRIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTITSSYRKIHLFDVDVPGGRVYKESSY 180

Query: 215 TKAGEDIVAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPSAFTKETGEAHWEI 274
           TKAGE IVAVDSPIGRLGLTVCYDLRFPEL  QLRFQHNA+VLLVPSAFTKETGEAHWEI
Sbjct: 181 TKAGEHIVAVDSPIGRLGLTVCYDLRFPELYLQLRFQHNAKVLLVPSAFTKETGEAHWEI 240

Query: 275 LLRSRAIENQCY 284
           LLRSRAIENQCY
Sbjct: 241 LLRSRAIENQCY 252

BLAST of ClCG02G013715 vs. ExPASy TrEMBL
Match: A0A5A7SST9 (Nitrilase-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00140 PE=4 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 6.2e-127
Identity = 229/252 (90.87%), Postives = 235/252 (93.25%), Query Frame = 0

Query: 35  MSVPPWLSPKHNLIQFTTPTASARNHLPLIRSNFAN---GGLADMAVNSVRVAAAQMTSI 94
           MSV PWLSPK NLI+F TPTASAR HLPLIRSNFAN   GG ADMAVNSVRVA AQMTS+
Sbjct: 1   MSVSPWLSPKQNLIRFLTPTASARYHLPLIRSNFANAFAGGSADMAVNSVRVAVAQMTSV 60

Query: 95  NDLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGPIMNQYCSLA 154
           N+LSSNFA CSRLVKEA SAGAKLICFPEDFSFMPA +GESQKIAEPLDGPIMNQYCSLA
Sbjct: 61  NNLSSNFATCSRLVKEAASAGAKLICFPEDFSFMPASEGESQKIAEPLDGPIMNQYCSLA 120

Query: 155 RESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVDIPGGRVYKESSY 214
           RES IWLSLGGFQEKGPDDQHFYNTHVIVDDTGTI SSYRKIHLFDVD+PGGRVYKESSY
Sbjct: 121 RESRIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTITSSYRKIHLFDVDVPGGRVYKESSY 180

Query: 215 TKAGEDIVAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPSAFTKETGEAHWEI 274
           TKAGE IVAVDSPIGRLGLTVCYDLRFPEL  QLRFQHNA+VLLVPSAFTKETGEAHWEI
Sbjct: 181 TKAGEHIVAVDSPIGRLGLTVCYDLRFPELYLQLRFQHNAKVLLVPSAFTKETGEAHWEI 240

Query: 275 LLRSRAIENQCY 284
           LLRSRAIENQCY
Sbjct: 241 LLRSRAIENQCY 252

BLAST of ClCG02G013715 vs. ExPASy TrEMBL
Match: A0A6J1H045 (nitrilase-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111458396 PE=4 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 1.4e-126
Identity = 235/311 (75.56%), Postives = 254/311 (81.67%), Query Frame = 0

Query: 35  MSVPPWLSPKHNLIQFTTPTASARNHLPLIRSNFANG--GLADMAVNSVRVAAAQMTSIN 94
           MSV PWLSP   LI+FT PT SAR+HLPLIRSNFA    G +DMAVNSVRVA AQMTSIN
Sbjct: 1   MSVSPWLSPNQTLIRFTIPTVSARDHLPLIRSNFATAFTGGSDMAVNSVRVAVAQMTSIN 60

Query: 95  DLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGPIMNQYCSLAR 154
           DLS NFA CSRLVKEAVSAGAKL+CFPEDFSFMPAKDGE QKIAEPLDGPIMNQYCSLAR
Sbjct: 61  DLSFNFATCSRLVKEAVSAGAKLVCFPEDFSFMPAKDGEGQKIAEPLDGPIMNQYCSLAR 120

Query: 155 ESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVDIPGGRVYKESSYT 214
           ESSIWLSLGGFQEKGPDD+HFYNTHVIVDD+GTIRS+YRKIHLFDVD+PGGRVYKESSYT
Sbjct: 121 ESSIWLSLGGFQEKGPDDEHFYNTHVIVDDSGTIRSTYRKIHLFDVDVPGGRVYKESSYT 180

Query: 215 KAGEDIVAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPSAFTKETGEAHWEIL 274
           KAGEDIVAVDSPIGRLGLTVCYDLRFPEL QQLRFQHN+QVLLVPSAFTKETGEAHWEIL
Sbjct: 181 KAGEDIVAVDSPIGRLGLTVCYDLRFPELYQQLRFQHNSQVLLVPSAFTKETGEAHWEIL 240

Query: 275 LRSRAIENQCY----------------------FERFFAPVSEISESVLEGTFINELDPE 322
           LRSRAIENQCY                       + +   V  +S+ +  G  + ++D +
Sbjct: 241 LRSRAIENQCYVIAAAQAGKANGKRESYGDSLIIDPWGKIVGRLSDRLATGITVADIDFD 300

BLAST of ClCG02G013715 vs. ExPASy TrEMBL
Match: A0A0A0KLN1 (CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G177080 PE=4 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 2.4e-126
Identity = 229/252 (90.87%), Postives = 235/252 (93.25%), Query Frame = 0

Query: 35  MSVPPWLSPKHNLIQFTTPTASARNHLPLIRSNFAN---GGLADMAVNSVRVAAAQMTSI 94
           MSV PWLSPK NLI+FTTPTAS R +LPLIRSNFAN   GG ADMAVNSVRVA AQMTS+
Sbjct: 1   MSVSPWLSPKQNLIRFTTPTASPRYNLPLIRSNFANALAGGSADMAVNSVRVAVAQMTSV 60

Query: 95  NDLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGPIMNQYCSLA 154
           N+LSSNFA CSRLVKEAVSAGAKLICFPEDFSFMPA +GESQKIAEPLDGPIMNQYCSLA
Sbjct: 61  NNLSSNFATCSRLVKEAVSAGAKLICFPEDFSFMPASEGESQKIAEPLDGPIMNQYCSLA 120

Query: 155 RESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVDIPGGRVYKESSY 214
           RES IWLSLGGFQEKGPDDQHFYNTHVIVDDTGTI SSYRKIHLFDVD+PGGRVYKESSY
Sbjct: 121 RESRIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTITSSYRKIHLFDVDVPGGRVYKESSY 180

Query: 215 TKAGEDIVAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPSAFTKETGEAHWEI 274
           TKAGE IVAVDSPIGRLG TVCYDLRFPEL  QLRFQHNAQVLLVPSAFTKETGEAHWEI
Sbjct: 181 TKAGEHIVAVDSPIGRLGPTVCYDLRFPELYLQLRFQHNAQVLLVPSAFTKETGEAHWEI 240

Query: 275 LLRSRAIENQCY 284
           LLRSRAIENQCY
Sbjct: 241 LLRSRAIENQCY 252

BLAST of ClCG02G013715 vs. ExPASy TrEMBL
Match: A0A6J1JPX3 (nitrilase-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111488776 PE=4 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 4.4e-125
Identity = 233/311 (74.92%), Postives = 253/311 (81.35%), Query Frame = 0

Query: 35  MSVPPWLSPKHNLIQFTTPTASARNHLPLIRSNFANG--GLADMAVNSVRVAAAQMTSIN 94
           MSV PWLSP   LI+FT PT SAR+HLPLIRSNFA    G +DMAVNSVRVA AQMTSIN
Sbjct: 1   MSVSPWLSPNQTLIRFTIPTVSARDHLPLIRSNFATAFTGGSDMAVNSVRVAVAQMTSIN 60

Query: 95  DLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGPIMNQYCSLAR 154
           +LS NFA CSRLVKEAVSAGAKL+CFPEDFSFMPAKDGE QKIAEPLDGPIMNQYCSLAR
Sbjct: 61  NLSFNFATCSRLVKEAVSAGAKLVCFPEDFSFMPAKDGEGQKIAEPLDGPIMNQYCSLAR 120

Query: 155 ESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVDIPGGRVYKESSYT 214
           ESSIWLSLGGFQEKGPDD+HFYNTHVIVDD+GTIRS+YRKIHLFDVD+PGGRVY+ESSYT
Sbjct: 121 ESSIWLSLGGFQEKGPDDEHFYNTHVIVDDSGTIRSTYRKIHLFDVDVPGGRVYRESSYT 180

Query: 215 KAGEDIVAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPSAFTKETGEAHWEIL 274
           KAGEDIVAVDSPIGRLGLTVCYDLRFPEL QQLRFQHNAQVLLVPSAFT ETGEAHWEIL
Sbjct: 181 KAGEDIVAVDSPIGRLGLTVCYDLRFPELYQQLRFQHNAQVLLVPSAFTIETGEAHWEIL 240

Query: 275 LRSRAIENQCY----------------------FERFFAPVSEISESVLEGTFINELDPE 322
           LRSRAIENQCY                       + +   V  +S+ +  G  + ++D +
Sbjct: 241 LRSRAIENQCYVIAAAQAGKANGKRESYGDSLIIDPWGKIVGRLSDRLATGITVADIDFD 300

BLAST of ClCG02G013715 vs. TAIR 10
Match: AT4G08790.1 (Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein )

HSP 1 Score: 321.6 bits (823), Expect = 9.6e-88
Identity = 167/301 (55.48%), Postives = 207/301 (68.77%), Query Frame = 0

Query: 48  IQFTTPTASAR----NHLPLIRSNFANGGLADMAVNSVRVAAAQMTSINDLSSNFAICSR 107
           + FT P+   R    + +PL  +   N         +VRVAAAQMTS+NDL +NFA CSR
Sbjct: 8   LDFTKPSLFTRITLSSQIPLTMATTVN--------KTVRVAAAQMTSVNDLMTNFATCSR 67

Query: 108 LVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLDGPIMNQYCSLARESSIWLSLGGF 167
           LV+EA  AGAKLICFPE+FSF+  K+GES KIAEPLDGP+M +YCSLAR+S+IWLSLGGF
Sbjct: 68  LVQEAALAGAKLICFPENFSFVGDKEGESVKIAEPLDGPVMERYCSLARDSNIWLSLGGF 127

Query: 168 QEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFDVDIPGGRVYKESSYTKAGEDIVAVDS 227
           QE+  DD H  NTHV++DD G IR +Y+K+HLFDVD+PGG  YKESS+T  G  IV+VDS
Sbjct: 128 QERF-DDTHLCNTHVVIDDAGMIRDTYQKMHLFDVDVPGGSSYKESSFTVPGTKIVSVDS 187

Query: 228 PIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVPSAFTKETGEAHWEILLRSRAIENQCY 287
           P+GRLGLTVCYDLRFP++ QQLRF+  AQVLLVPSAFTK TGEAHWEILLR+RAIE QCY
Sbjct: 188 PVGRLGLTVCYDLRFPKIYQQLRFEQKAQVLLVPSAFTKVTGEAHWEILLRARAIETQCY 247

Query: 288 ----------------------FERFFAPVSEISESVLEGTFINELDPELKAKVESRNPV 323
                                  + +   V  + + V  G  + ++D  L   V ++ P+
Sbjct: 248 VIAAAQAGKHNEKRESYGDTLIIDPWGTVVGRLPDRVSTGIVVADIDFSLIDSVRTKMPI 299

BLAST of ClCG02G013715 vs. TAIR 10
Match: AT5G12040.1 (Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein )

HSP 1 Score: 127.9 bits (320), Expect = 2.0e-29
Identity = 70/206 (33.98%), Postives = 106/206 (51.46%), Query Frame = 0

Query: 83  VAAAQMTSINDLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLD-- 142
           +   Q++  +D   N +   + ++EA S GAKL+  PE ++  P  +      AE +D  
Sbjct: 90  IGLCQLSVTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWN-SPYSNDSFPVYAEEIDAG 149

Query: 143 ---GPIMNQYCSLARESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFD 202
               P       +++   I +  G   E+  D    YNT  +    G +++ +RKIHLFD
Sbjct: 150 GDASPSTAMLSEVSKRLKITIIGGSIPERVGD--RLYNTCCVFGSDGELKAKHRKIHLFD 209

Query: 203 VDIPGGRVYKESSYTKAGEDIVAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVP 262
           +DIPG   + ES    AGE    VD+ +GR+G+ +CYD+RF EL   +     A +L  P
Sbjct: 210 IDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELA-MIYAARGAHLLCYP 269

Query: 263 SAFTKETGEAHWEILLRSRAIENQCY 284
            AF   TG  HWE+L R+RA +NQ Y
Sbjct: 270 GAFNMTTGPLHWELLQRARATDNQLY 291

BLAST of ClCG02G013715 vs. TAIR 10
Match: AT5G12040.2 (Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein )

HSP 1 Score: 125.9 bits (315), Expect = 7.7e-29
Identity = 69/206 (33.50%), Postives = 106/206 (51.46%), Query Frame = 0

Query: 83  VAAAQMTSINDLSSNFAICSRLVKEAVSAGAKLICFPEDFSFMPAKDGESQKIAEPLD-- 142
           +   Q++  +D   N +   + ++EA S GAKL+  PE ++  P  +      AE +D  
Sbjct: 90  IGLCQLSVTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWN-SPYSNDSFPVYAEEIDAG 149

Query: 143 ---GPIMNQYCSLARESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLFD 202
               P       +++   I +  G   E+  D    YNT  +    G +++ +RKIHLFD
Sbjct: 150 GDASPSTAMLSEVSKRLKITIIGGSIPERVGD--RLYNTCCVFGSDGELKAKHRKIHLFD 209

Query: 203 VDIPGGRVYKESSYTKAGEDIVAVDSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLLVP 262
           +DIPG   + ES    AGE    VD+ +GR+G+ +CYD+RF EL   +     A +L  P
Sbjct: 210 IDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELA-MIYAARGAHLLCYP 269

Query: 263 SAFTKETGEAHWEILLRSRAIENQCY 284
            AF   TG  HWE+L R+RA +NQ +
Sbjct: 270 GAFNMTTGPLHWELLQRARATDNQVH 291

BLAST of ClCG02G013715 vs. TAIR 10
Match: AT2G27450.1 (nitrilase-like protein 1 )

HSP 1 Score: 82.0 bits (201), Expect = 1.3e-15
Identity = 62/211 (29.38%), Postives = 101/211 (47.87%), Query Frame = 0

Query: 81  VRVAAAQMTSINDLSSNFAICSRLVKEAVSAGAKLICFPEDFS---FMPAKDGESQKIAE 140
           V V++ Q    +D+S+N A   RLV+EA + GA +I   E F    F  A+  +  K A+
Sbjct: 10  VVVSSLQFACSDDISTNVAAAERLVREAHAKGANIILIQELFEGYYFCQAQREDFFKRAK 69

Query: 141 PL-DGPIMNQYCSLARESSIWLSLGGFQEKGPDDQHFYNTHVIVDDTGTIRSSYRKIHLF 200
           P  + P + +   LA+E  + + +  F+E    +   YN+  I+D  GT    YRK H  
Sbjct: 70  PYKNHPTIARMQKLAKELGVVIPVSFFEEA---NTAHYNSIAIIDADGTDLGIYRKSH-- 129

Query: 201 DVDIPGGRVYKESSYTKAGEDIVAV-DSPIGRLGLTVCYDLRFPELCQQLRFQHNAQVLL 260
              IP G  Y+E  Y   G+    V  +   ++G+ +C+D  FPE  + +  Q  A++L 
Sbjct: 130 ---IPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAICWDQWFPEAARAMVLQ-GAEILF 189

Query: 261 VPSAFTKETGEA------HWEILLRSRAIEN 281
            P+A   E  +       HW  +++  A  N
Sbjct: 190 YPTAIGSEPQDQGLDSRDHWRRVMQGHAGAN 211

BLAST of ClCG02G013715 vs. TAIR 10
Match: AT3G44310.1 (nitrilase 1 )

HSP 1 Score: 57.8 bits (138), Expect = 2.6e-08
Identity = 50/223 (22.42%), Postives = 96/223 (43.05%), Query Frame = 0

Query: 80  SVRVAAAQMTSI-NDLSSNFAICSRLVKEAVSAGAKLICFPEDF-------------SFM 139
           +VRV   Q +++ ND  +      + + EA S GA+L+ FPE F               +
Sbjct: 24  TVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGV 83

Query: 140 PAKDGESQ-----KIAEPLDGPIMNQYCSLARESSIWLSLGGFQEKGPDDQHFYNTHVIV 199
             ++G  +       A  + GP + +   +AR++ ++L +G  +++G      Y T +  
Sbjct: 84  HNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEG---YTLYCTVLFF 143

Query: 200 DDTGTIRSSYRKIHLFDVDIPGGRVYKESSYTKAGEDIVAVDSPIGRLGLTVCYDLRFPE 259
              G     +RK+    ++     ++ +      G  I   D+PIG+LG  +C++ R P 
Sbjct: 144 SPQGQFLGKHRKLMPTSLE---RCIWGQGD----GSTIPVYDTPIGKLGAAICWENRMP- 203

Query: 260 LCQQLRFQHNAQVLLVPSAFTKETGEAHWEILLRSRAIENQCY 284
           L +   +    ++   P+A     G   W+  +   AIE  C+
Sbjct: 204 LYRTALYAKGIELYCAPTA----DGSKEWQSSMLHIAIEGGCF 231

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893375.11.0e-13179.17deaminated glutathione amidase, chloroplastic/cytosolic [Benincasa hispida][more]
XP_008445813.11.3e-12690.87PREDICTED: nitrilase-like protein 2 [Cucumis melo] >XP_008445815.1 PREDICTED: ni... [more]
KAA0034030.11.3e-12690.87nitrilase-like protein 2 [Cucumis melo var. makuwa][more]
XP_023525991.12.9e-12675.56nitrilase-like protein 2 [Cucurbita pepo subsp. pepo][more]
XP_022956809.12.9e-12675.56nitrilase-like protein 2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q94JV51.3e-8655.48Deaminated glutathione amidase, chloroplastic/cytosolic OS=Arabidopsis thaliana ... [more]
Q557J51.8e-5151.67Deaminated glutathione amidase OS=Dictyostelium discoideum OX=44689 GN=nit1-1 PE... [more]
Q8VDK11.6e-5049.27Deaminated glutathione amidase OS=Mus musculus OX=10090 GN=Nit1 PE=1 SV=2[more]
Q7TQ944.5e-5049.27Deaminated glutathione amidase OS=Rattus norvegicus OX=10116 GN=Nit1 PE=2 SV=2[more]
Q32LH42.2e-4941.11Deaminated glutathione amidase OS=Bos taurus OX=9913 GN=NIT1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BDK36.2e-12790.87nitrilase-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103488728 PE=4 SV=1[more]
A0A5A7SST96.2e-12790.87Nitrilase-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A6J1H0451.4e-12675.56nitrilase-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111458396 PE=4 SV=1[more]
A0A0A0KLN12.4e-12690.87CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G17708... [more]
A0A6J1JPX34.4e-12574.92nitrilase-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111488776 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G08790.19.6e-8855.48Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein [more]
AT5G12040.12.0e-2933.98Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein [more]
AT5G12040.27.7e-2933.50Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein [more]
AT2G27450.11.3e-1529.38nitrilase-like protein 1 [more]
AT3G44310.12.6e-0822.42nitrilase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 323..343
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 401..420
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 351..436
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 377..397
NoneNo IPR availablePANTHERPTHR23088:SF27DEAMINATED GLUTATHIONE AMIDASEcoord: 79..285
NoneNo IPR availablePANTHERPTHR23088NITRILASE-RELATEDcoord: 79..285
NoneNo IPR availableCDDcd07572nitcoord: 82..283
e-value: 5.99312E-108
score: 317.832
IPR003010Carbon-nitrogen hydrolasePFAMPF00795CN_hydrolasecoord: 82..283
e-value: 2.9E-42
score: 144.8
IPR003010Carbon-nitrogen hydrolasePROSITEPS50263CN_HYDROLASEcoord: 81..323
score: 47.24025
IPR036526Carbon-nitrogen hydrolase superfamilyGENE3D3.60.110.10coord: 81..321
e-value: 3.6E-63
score: 215.5
IPR036526Carbon-nitrogen hydrolase superfamilySUPERFAMILY56317Carbon-nitrogen hydrolasecoord: 78..284

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG02G013715.1ClCG02G013715.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0110051 metabolite repair
biological_process GO:0006807 nitrogen compound metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0110050 deaminated glutathione amidase activity