ClCG02G000597 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG02G000597
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionPectinesterase
LocationCG_Chr02: 625842 .. 628920 (+)
RNA-Seq ExpressionClCG02G000597
SyntenyClCG02G000597
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCAAATTTTTTTAAAAAAATGAAAATAGAAAGTCCCAACGCAAGAGAGAGTACTTTTCGTTATTCTCTTTTATGACATTGAAAGAAGGAAAACGAAAAAACGCACAGAAAATTCCAATTCCTCCGCCGTAGACTGCAGCGGTCACCACTTTAACCGCCTCCACAACCACCCGTGACTTTTGTTTTCGTCTCCCCGTGATTCCATTTCTCCATTTTTGGCCATTATCAGCACCACCATTAATCTCTCTCTCTCTCCACATTCTCCATTATAAATAATTCCGTTTCCATTTCCCACAATGCTCCCCAGAAAACCCCCATTCCCTTTCCCCATTTCCAAATCCACTCTCCTTTTCCTCTCCATGGCTTCCCTCTCCTTCCTCTCTTTCCTCTCCCTCTTCTTCTTCTTCCTCTCTCTCTCCTCCTCCTCCGCCGCCGCTCGCCATCGCCACCACCACCTCCCAACCCATTTCACCAACCCCCCTTCCACCGACGACGTCATCCTTCACGCCTGCAAGGCCACCCGTTTTCCCGATCAATGCGGATCTTCCCTCTCTCACTCCTCCTCTCTCCCTCCCAATCCTACCCCTCTCCAGATCCTCCAATCCGCCGTCGCCCTCTCATCCGACGGTTTAGCCACTGCTCAATCCATGGTTAACCAGATTCTCGATTCCTCCGCCAAGAATCTCAATCGGACCACCGCTGCCAAGAACTGCCTTGAGGTACTGCGGAATTCCGATTACCGTATTTCTCTCACCAACGACGCTCTCCGCCGTGGTCGGACCAAGGACGCCCGCGCCTGGCTGAGTGCGTCCCTACTTTACCATTACGATTGCTGGTCTGCGCTCAAGTACGCCAACGACACTCAATTGGTTGACAAAACCATGGCGTTTCTCGATTCTCTTATTGGTCTCTCCAGCAACGCGCTGAGTATGTTGGTGGCTTACGACATTTCCGGCAACGATACGGCGGCGTGGACGCCGCCGAGGACTGAGCGGGATGGGTTTTGGGAAGGCGGTGGTGGCGGATCTGGGTTGGGGTTTAGCGGTGGGTTTCCGTCGAAGGTGGCGGCTGATGCGACGGTGTGCAAGGATGGCGCTGGGGGATGTTATAAAACTGTTCAGGAAGCTGTTAACGCGGCGCCGGAGAATGCGGCGGCGCGTAGGTTTGTGATTCATATTAAGGAAGGTGTTTATGAGGAAATCGTGAGGGTTCCGTTAGAGAAGAAGAATGTGGTGTTTTTAGGAGATGGGATGGGCAAAACGGTCATTACGGGCTCACTTAATGTGGGCCAGCCTGGGATTTCCACTTACAACACCGCCACCGTTGGTGAGTTTTTTATTTTTTTATTTTTTAATAATAATAATTTATTTTTTAAATTTAAATTTTCAATTTTTTTTTCAATTTATAATTTTGTGTCAATTTTATGTTTAGTATAACTTTAAATTTCAGAGATAATTTCTTTTAGAAGTGAAAGGATCAAATTACTCTAACACCAATAAATACAAATTTAAAATTTAGACTAAATTGATAATTTAACGGATCAAATCTTAGCTTACATGTGGTTTCATGTTTGATTAGGAACTTGATGAGTGCAAATTTGATTGTTTTTCATTACCATTTGAATTTACTTTATCAAACACGCCATAAATTATTGCATTTATGTATTCCTTTCCATTTCGAATTTTGGCTAATGGGATTTTCCTTTCTATTTTCCTTTCTAGTTTCCTTCCTAAATTTGGGGGAAAAAAAAAAAGGAAAATCATGCATTTGATTACACTCATTTTAACGATTACGCTATGGTTAGTGGTCAAATCACCACAGGGGTGGGTGGCCTATGGCTAATCAAGGCCTTCTAGAAAATTGATTTAACTTACTGTCATCCACTACTTAACTACTTAAGATTTAATATCTTACAGATTTACATACGCTTGAAGATGTCTAAAATATTAATGTTAGTCTCGAATAAGTCTTCTATTCTTAAGATTCTTATTGTTAAAACTAAGTAGTTAAAATTGGTTTATATATTGATAATCTGAATTCTTTATGGTTGTGTATTGATCAAAAGAGGTATGGATGACCCGAATTCTTTCTGTTCGATGTGATGGAAGTGGTATATGGTTGGATAATTAAAGATTGAAAAATGTTGGGAATTCAGTATAAAGTTGATGTTGTTATGTGCAGGAGTACTTGGTGATGGATTCATGGCTAGCGGTCTCACCATTCAAAACACAGCAGGGCCAGATGCTCATCAAGCTGTGGCTTTTAGATCAGACAGTGATCTCTCAGTTATTCAAGACTGTGAGTTTCTAGGCAATCAAGACACTCTCTATGCTCACTCCCTTCGCCAATTCTACAAATCTTGTAAGATTCAAGGCAATGTAGATTTCATCTTTGGAAACTCAGCTTCCATCTTCCAAGACTGCGAGATCCTAGTTCGCCCCAGACAGCTCAAGCCTGAGAAAGGTGAAAACAACGCCATCACTGCTCAGGGCAGGACCGACCCTGCTCAAGCGACAGGGTTCGTATTCCAAAACTGCCTCATAAATGGCACTCAGGAGTACATGTCATTGTACTACAGCAAGCCTAAAGTGCATAAAAACTACTTGGGGAGACCGTGGAAGGAGTACTCGAGGACAGTTTTTATACATTGTACTCTCGAGGCTCTTGTCACGCCTGCGGGGTGGATGCCATGGAGTGGGGATTTTGCATTGAAGACTCTTTTCTATGGAGAGTTTGAGAATTCGGGAGCGGGGTCGAAGACATCGGAGAGAGTCGGGTGGAGTAGTCAGATTCCAGCACAACATGTTTTTAGTTACTCTGTTCAAAATTTCATTCAGGGAGACGAATGGATTCCTTCATCTCAGTCATCTAATGAACCAAAAATGTTGAACAAGAAAAGTAAAGATGTGAAATTAGATTAGATTTACAAATCCCTTTGTAATTACAAACTTCTCTATGGAACTACATATTATTAGTCTACATAAAACTACCAAATAATCCACCACCCATCTCTTTCTCATTTTCAAATTTGGATATATGTGTATATATATATTTCTTATATGATGGTTGTTGATTGAAA

mRNA sequence

TTCAAATTTTTTTAAAAAAATGAAAATAGAAAGTCCCAACGCAAGAGAGAGTACTTTTCGTTATTCTCTTTTATGACATTGAAAGAAGGAAAACGAAAAAACGCACAGAAAATTCCAATTCCTCCGCCGTAGACTGCAGCGGTCACCACTTTAACCGCCTCCACAACCACCCGTGACTTTTGTTTTCGTCTCCCCGTGATTCCATTTCTCCATTTTTGGCCATTATCAGCACCACCATTAATCTCTCTCTCTCTCCACATTCTCCATTATAAATAATTCCGTTTCCATTTCCCACAATGCTCCCCAGAAAACCCCCATTCCCTTTCCCCATTTCCAAATCCACTCTCCTTTTCCTCTCCATGGCTTCCCTCTCCTTCCTCTCTTTCCTCTCCCTCTTCTTCTTCTTCCTCTCTCTCTCCTCCTCCTCCGCCGCCGCTCGCCATCGCCACCACCACCTCCCAACCCATTTCACCAACCCCCCTTCCACCGACGACGTCATCCTTCACGCCTGCAAGGCCACCCGTTTTCCCGATCAATGCGGATCTTCCCTCTCTCACTCCTCCTCTCTCCCTCCCAATCCTACCCCTCTCCAGATCCTCCAATCCGCCGTCGCCCTCTCATCCGACGGTTTAGCCACTGCTCAATCCATGGTTAACCAGATTCTCGATTCCTCCGCCAAGAATCTCAATCGGACCACCGCTGCCAAGAACTGCCTTGAGGTACTGCGGAATTCCGATTACCGTATTTCTCTCACCAACGACGCTCTCCGCCGTGGTCGGACCAAGGACGCCCGCGCCTGGCTGAGTGCGTCCCTACTTTACCATTACGATTGCTGGTCTGCGCTCAAGTACGCCAACGACACTCAATTGGTTGACAAAACCATGGCGTTTCTCGATTCTCTTATTGGTCTCTCCAGCAACGCGCTGAGTATGTTGGTGGCTTACGACATTTCCGGCAACGATACGGCGGCGTGGACGCCGCCGAGGACTGAGCGGGATGGGTTTTGGGAAGGCGGTGGTGGCGGATCTGGGTTGGGGTTTAGCGGTGGGTTTCCGTCGAAGGTGGCGGCTGATGCGACGGTGTGCAAGGATGGCGCTGGGGGATGTTATAAAACTGTTCAGGAAGCTGTTAACGCGGCGCCGGAGAATGCGGCGGCGCGTAGGTTTGTGATTCATATTAAGGAAGGTGTTTATGAGGAAATCGTGAGGGTTCCGTTAGAGAAGAAGAATGTGGTGTTTTTAGGAGATGGGATGGGCAAAACGGTCATTACGGGCTCACTTAATGTGGGCCAGCCTGGGATTTCCACTTACAACACCGCCACCGTTGAAGAGGTATGGATGACCCGAATTCTTTCTGTTCGATGTGATGGAAGTGGAGTACTTGGTGATGGATTCATGGCTAGCGGTCTCACCATTCAAAACACAGCAGGGCCAGATGCTCATCAAGCTGTGGCTTTTAGATCAGACAGTGATCTCTCAGTTATTCAAGACTGTGAGTTTCTAGGCAATCAAGACACTCTCTATGCTCACTCCCTTCGCCAATTCTACAAATCTTGTAAGATTCAAGGCAATGTAGATTTCATCTTTGGAAACTCAGCTTCCATCTTCCAAGACTGCGAGATCCTAGTTCGCCCCAGACAGCTCAAGCCTGAGAAAGGTGAAAACAACGCCATCACTGCTCAGGGCAGGACCGACCCTGCTCAAGCGACAGGGTTCGTATTCCAAAACTGCCTCATAAATGGCACTCAGGAGTACATGTCATTGTACTACAGCAAGCCTAAAGTGCATAAAAACTACTTGGGGAGACCGTGGAAGGAGTACTCGAGGACAGTTTTTATACATTGTACTCTCGAGGCTCTTGTCACGCCTGCGGGGTGGATGCCATGGAGTGGGGATTTTGCATTGAAGACTCTTTTCTATGGAGAGTTTGAGAATTCGGGAGCGGGGTCGAAGACATCGGAGAGAGTCGGGTGGAGTAGTCAGATTCCAGCACAACATGTTTTTAGTTACTCTGTTCAAAATTTCATTCAGGGAGACGAATGGATTCCTTCATCTCAGTCATCTAATGAACCAAAAATGTTGAACAAGAAAAGTAAAGATGTGAAATTAGATTAGATTTACAAATCCCTTTGTAATTACAAACTTCTCTATGGAACTACATATTATTAGTCTACATAAAACTACCAAATAATCCACCACCCATCTCTTTCTCATTTTCAAATTTGGATATATGTGTATATATATATTTCTTATATGATGGTTGTTGATTGAAA

Coding sequence (CDS)

ATGCTCCCCAGAAAACCCCCATTCCCTTTCCCCATTTCCAAATCCACTCTCCTTTTCCTCTCCATGGCTTCCCTCTCCTTCCTCTCTTTCCTCTCCCTCTTCTTCTTCTTCCTCTCTCTCTCCTCCTCCTCCGCCGCCGCTCGCCATCGCCACCACCACCTCCCAACCCATTTCACCAACCCCCCTTCCACCGACGACGTCATCCTTCACGCCTGCAAGGCCACCCGTTTTCCCGATCAATGCGGATCTTCCCTCTCTCACTCCTCCTCTCTCCCTCCCAATCCTACCCCTCTCCAGATCCTCCAATCCGCCGTCGCCCTCTCATCCGACGGTTTAGCCACTGCTCAATCCATGGTTAACCAGATTCTCGATTCCTCCGCCAAGAATCTCAATCGGACCACCGCTGCCAAGAACTGCCTTGAGGTACTGCGGAATTCCGATTACCGTATTTCTCTCACCAACGACGCTCTCCGCCGTGGTCGGACCAAGGACGCCCGCGCCTGGCTGAGTGCGTCCCTACTTTACCATTACGATTGCTGGTCTGCGCTCAAGTACGCCAACGACACTCAATTGGTTGACAAAACCATGGCGTTTCTCGATTCTCTTATTGGTCTCTCCAGCAACGCGCTGAGTATGTTGGTGGCTTACGACATTTCCGGCAACGATACGGCGGCGTGGACGCCGCCGAGGACTGAGCGGGATGGGTTTTGGGAAGGCGGTGGTGGCGGATCTGGGTTGGGGTTTAGCGGTGGGTTTCCGTCGAAGGTGGCGGCTGATGCGACGGTGTGCAAGGATGGCGCTGGGGGATGTTATAAAACTGTTCAGGAAGCTGTTAACGCGGCGCCGGAGAATGCGGCGGCGCGTAGGTTTGTGATTCATATTAAGGAAGGTGTTTATGAGGAAATCGTGAGGGTTCCGTTAGAGAAGAAGAATGTGGTGTTTTTAGGAGATGGGATGGGCAAAACGGTCATTACGGGCTCACTTAATGTGGGCCAGCCTGGGATTTCCACTTACAACACCGCCACCGTTGAAGAGGTATGGATGACCCGAATTCTTTCTGTTCGATGTGATGGAAGTGGAGTACTTGGTGATGGATTCATGGCTAGCGGTCTCACCATTCAAAACACAGCAGGGCCAGATGCTCATCAAGCTGTGGCTTTTAGATCAGACAGTGATCTCTCAGTTATTCAAGACTGTGAGTTTCTAGGCAATCAAGACACTCTCTATGCTCACTCCCTTCGCCAATTCTACAAATCTTGTAAGATTCAAGGCAATGTAGATTTCATCTTTGGAAACTCAGCTTCCATCTTCCAAGACTGCGAGATCCTAGTTCGCCCCAGACAGCTCAAGCCTGAGAAAGGTGAAAACAACGCCATCACTGCTCAGGGCAGGACCGACCCTGCTCAAGCGACAGGGTTCGTATTCCAAAACTGCCTCATAAATGGCACTCAGGAGTACATGTCATTGTACTACAGCAAGCCTAAAGTGCATAAAAACTACTTGGGGAGACCGTGGAAGGAGTACTCGAGGACAGTTTTTATACATTGTACTCTCGAGGCTCTTGTCACGCCTGCGGGGTGGATGCCATGGAGTGGGGATTTTGCATTGAAGACTCTTTTCTATGGAGAGTTTGAGAATTCGGGAGCGGGGTCGAAGACATCGGAGAGAGTCGGGTGGAGTAGTCAGATTCCAGCACAACATGTTTTTAGTTACTCTGTTCAAAATTTCATTCAGGGAGACGAATGGATTCCTTCATCTCAGTCATCTAATGAACCAAAAATGTTGAACAAGAAAAGTAAAGATGTGAAATTAGATTAG

Protein sequence

MLPRKPPFPFPISKSTLLFLSMASLSFLSFLSLFFFFLSLSSSSAAARHRHHHLPTHFTNPPSTDDVILHACKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCDGSGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQNCLINGTQEYMSLYYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQSSNEPKMLNKKSKDVKLD
Homology
BLAST of ClCG02G000597 vs. NCBI nr
Match: XP_038902126.1 (probable pectinesterase/pectinesterase inhibitor 51 [Benincasa hispida])

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 559/605 (92.40%), Postives = 571/605 (94.38%), Query Frame = 0

Query: 1   MLPRKPPFPFPISKSTLLFLSMASLSFLSFLSLFFFFLSLSSSSAAARHRHHHLPTHFTN 60
           M+PRK    FPISKSTLLFLSMASLSFLSFLSLF FFLSLSS   AARHRHHHL THFTN
Sbjct: 1   MVPRKT--HFPISKSTLLFLSMASLSFLSFLSLFSFFLSLSS---AARHRHHHLSTHFTN 60

Query: 61  PPSTDDVILHACKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQSMVN 120
           PPS D  ILHACKATRFPDQCGSSLSH SSLPPNP+PLQILQSAV+LSSDGL+TAQSMV 
Sbjct: 61  PPSIDG-ILHACKATRFPDQCGSSLSH-SSLPPNPSPLQILQSAVSLSSDGLSTAQSMVK 120

Query: 121 QILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCW 180
           QILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCW
Sbjct: 121 QILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCW 180

Query: 181 SALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGG 240
           SALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDI GNDTAAWTPPRTERDGFWEGG
Sbjct: 181 SALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGG 240

Query: 241 GGGSGLGFSGGFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEGVYE 300
           GGGSGLGFSGGFPSK+AADATVCKDGAGGCY TVQEAV+AAPENAAARRFVIHIKEGVYE
Sbjct: 241 GGGSGLGFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYE 300

Query: 301 EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCDGSG 360
           EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPG+STYNTATV                G
Sbjct: 301 EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATV----------------G 360

Query: 361 VLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSC 420
           VLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEF+GNQDTLYAHSLRQFYKSC
Sbjct: 361 VLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSC 420

Query: 421 KIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQNCLI 480
           KIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQ+TGFVFQNCLI
Sbjct: 421 KIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLI 480

Query: 481 NGTQEYMSLYYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALKTLF 540
           NGTQEYMSLYYSKPKVHKN+LGRPWKEYSRTVFIHC LE+LVTPAGWMPWSGDFALKTLF
Sbjct: 481 NGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLF 540

Query: 541 YGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQSSNEPKMLNKKSK 600
           YGEFENSG GSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQ+SNEPKMLNKKSK
Sbjct: 541 YGEFENSGVGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQTSNEPKMLNKKSK 582

Query: 601 DVKLD 606
           DVKLD
Sbjct: 601 DVKLD 582

BLAST of ClCG02G000597 vs. NCBI nr
Match: XP_008460902.1 (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis melo])

HSP 1 Score: 1059.7 bits (2739), Expect = 9.6e-306
Identity = 541/609 (88.83%), Postives = 563/609 (92.45%), Query Frame = 0

Query: 1   MLPRKPPFPFPISKSTLLFLSMASLSFLSFLSL--FFFFLSLSSSSAAARHR--HHHLPT 60
           + PRKP   FPISKSTLLFLSMASLSFLSFLSL  FFF LSLSSSS+A RH   H HLP+
Sbjct: 2   VFPRKP--TFPISKSTLLFLSMASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLPS 61

Query: 61  HFTNPPSTDDVILHACKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQ 120
           HFTN PS  D ILHACK+TRFPDQCGSSLSHSS  PPNP+PLQILQSA+ALSSDGL TAQ
Sbjct: 62  HFTNTPS--DQILHACKSTRFPDQCGSSLSHSSXSPPNPSPLQILQSAIALSSDGLTTAQ 121

Query: 121 SMVNQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYH 180
           S+V QILDSSA NLNRTTAAKNCLEVLRNSDYRISLTNDAL RGRTKDARAWLSASLLYH
Sbjct: 122 SLVKQILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYH 181

Query: 181 YDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGF 240
           YDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDI GNDTAAWTPPRTERDGF
Sbjct: 182 YDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGF 241

Query: 241 WEGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKE 300
           WEGGGGGSGLGFSGGFP+KV ADATVCKDG+GGCY ++Q+AV+AAPENAAARRFVIHIKE
Sbjct: 242 WEGGGGGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKE 301

Query: 301 GVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRC 360
           GVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLN GQPGISTYNTAT+             
Sbjct: 302 GVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATL------------- 361

Query: 361 DGSGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQF 420
              GVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEF+GNQDTLYAHSLRQF
Sbjct: 362 ---GVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQF 421

Query: 421 YKSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQ 480
           YKSCKIQGNVDFIFGNSASIFQDCEIL+RPRQLKPEKGENNAITA GRTDPAQ+TGFVFQ
Sbjct: 422 YKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQ 481

Query: 481 NCLINGTQEYMSLYYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFAL 540
           NCLINGTQEYMSLYYSKPKVHKN+LGRPWKEYSRTVFIHC LEALV+P+GWMPWSGDFAL
Sbjct: 482 NCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFAL 541

Query: 541 KTLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQSSNEPKMLN 600
           KTLFYGEFENSG GSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSS+SSN+ KMLN
Sbjct: 542 KTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNDQKMLN 590

Query: 601 KKSKDVKLD 606
           KKSKDVKLD
Sbjct: 602 KKSKDVKLD 590

BLAST of ClCG02G000597 vs. NCBI nr
Match: XP_004147375.2 (probable pectinesterase/pectinesterase inhibitor 51 [Cucumis sativus] >KGN62146.1 hypothetical protein Csa_005971 [Cucumis sativus])

HSP 1 Score: 1051.2 bits (2717), Expect = 3.4e-303
Identity = 537/608 (88.32%), Postives = 564/608 (92.76%), Query Frame = 0

Query: 1   MLPRKPPFPFPISKSTLLFLSMASLSFLSFLSLFFFFLSLSSSSAAARHRHH---HLPTH 60
           + PRKP FPF  SKSTLLFLSMASLSFLSFLSLFFFF SLS SS++A H HH   HLP+H
Sbjct: 2   VFPRKPTFPF--SKSTLLFLSMASLSFLSFLSLFFFFSSLSLSSSSADHHHHPHPHLPSH 61

Query: 61  FTNPPSTDDVILHACKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQS 120
           FTN PS  D ILHACK+TRFPDQCGSSLSH SSLPPNP+PLQILQSA+ALSSDGL TAQS
Sbjct: 62  FTNYPS--DQILHACKSTRFPDQCGSSLSH-SSLPPNPSPLQILQSAIALSSDGLTTAQS 121

Query: 121 MVNQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHY 180
           +V QILDSSA NLNRTTAAKNCLEVLRNSDYRISLTNDAL  GRTKDARAWLSASLLYHY
Sbjct: 122 LVKQILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHHGRTKDARAWLSASLLYHY 181

Query: 181 DCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFW 240
           DCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDI GNDTAAWTPPRTERDGFW
Sbjct: 182 DCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFW 241

Query: 241 EGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEG 300
           E GGGGSGLGFSGGFP+KV ADATVCKDG+GGCY ++Q+AV+AAPENAAARRFVIHIKEG
Sbjct: 242 E-GGGGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEG 301

Query: 301 VYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCD 360
           VYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPG+STYNTAT+              
Sbjct: 302 VYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATL-------------- 361

Query: 361 GSGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY 420
             GV+GDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY
Sbjct: 362 --GVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY 421

Query: 421 KSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQN 480
           KSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNA+TA GRTDPAQ+TGFVFQN
Sbjct: 422 KSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQSTGFVFQN 481

Query: 481 CLINGTQEYMSLYYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALK 540
           CLINGTQEYMSLYYSKP+VHKN+LGRPWKEYSRTVFIHC LEALV+P+GWMPWSGDFALK
Sbjct: 482 CLINGTQEYMSLYYSKPQVHKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALK 541

Query: 541 TLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQSSNEPKMLNK 600
           TLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSV+NFIQGDEWIPSS+SS+E KMLNK
Sbjct: 542 TLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVENFIQGDEWIPSSESSDEQKMLNK 587

Query: 601 KSKDVKLD 606
           +SKDVKLD
Sbjct: 602 RSKDVKLD 587

BLAST of ClCG02G000597 vs. NCBI nr
Match: XP_022932006.1 (probable pectinesterase/pectinesterase inhibitor 51 [Cucurbita moschata])

HSP 1 Score: 1006.5 bits (2601), Expect = 9.6e-290
Identity = 509/599 (84.97%), Postives = 541/599 (90.32%), Query Frame = 0

Query: 2   LPRKPPFPFPI----SKSTLLFLSMASLSFLSFLSLFFFFLSLSSSSAAARHRHHHLPTH 61
           LPR  PFPFP     +KST LFLSMA+LSFLSFLSL FFFLSL SS+   RH HHHLP+H
Sbjct: 5   LPRTTPFPFPFQFNAAKSTFLFLSMAALSFLSFLSL-FFFLSL-SSAVHHRHHHHHLPSH 64

Query: 62  FTNPPSTDDVILHACKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQS 121
           FTN PS+++    ACKATRFPDQCGSSLSHS SLPPNPT LQI+QSA+A+SSDGLA+A+S
Sbjct: 65  FTNSPSSEET-FRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKS 124

Query: 122 MVNQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHY 181
           MV QILDSS  + NRTTAAKNCLEVLR S+YRISLTNDA+RRGRTKDARAW+SASLLYHY
Sbjct: 125 MVKQILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHY 184

Query: 182 DCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFW 241
           DCWSALKYANDTQ V+KTMAFLDSLIGL+SNALSML AYDISGNDTAAWTPPRTERDGFW
Sbjct: 185 DCWSALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTPPRTERDGFW 244

Query: 242 EGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEG 301
           EG  GGSGLGFSGGFPSKV A ATVCKDGAGGCYKTVQEAVNAAP+NA  +RFVIHIKEG
Sbjct: 245 EGSSGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEG 304

Query: 302 VYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCD 361
           VY+EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATV              
Sbjct: 305 VYDEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATV-------------- 364

Query: 362 GSGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY 421
             GV+GDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY
Sbjct: 365 --GVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY 424

Query: 422 KSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQN 481
           KSCKIQGNVDFIFGNSAS+FQDCEILVRPRQLKPEKGENNA+TA GRTDPAQ TGFVFQN
Sbjct: 425 KSCKIQGNVDFIFGNSASVFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQN 484

Query: 482 CLINGTQEYMSLYYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALK 541
           CL+NGTQEYM+LYYSKPKVHKN+LGRPWKEYSRTVFIHC LEALV+PAGWMPW+GDFALK
Sbjct: 485 CLVNGTQEYMALYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALK 544

Query: 542 TLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQSSNEPKMLN 597
           TL+YGEFENSG GSKTSERV WSSQIPAQHV+SYSVQNFIQGDEWIPSSQSS E K +N
Sbjct: 545 TLYYGEFENSGTGSKTSERVEWSSQIPAQHVYSYSVQNFIQGDEWIPSSQSSEESKTIN 584

BLAST of ClCG02G000597 vs. NCBI nr
Match: KAG6571352.1 (putative pectinesterase/pectinesterase inhibitor 51, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 999.6 bits (2583), Expect = 1.2e-287
Identity = 507/599 (84.64%), Postives = 539/599 (89.98%), Query Frame = 0

Query: 2   LPRKPPFPFPI----SKSTLLFLSMASLSFLSFLSLFFFFLSLSSSSAAARHRHHHLPTH 61
           LPR  PF FP     +KST LFLSMA+LSFLSFLSL FFFLSL SS+   RH H+HLP+H
Sbjct: 5   LPRTTPFSFPFQFTTAKSTFLFLSMAALSFLSFLSL-FFFLSL-SSAVHHRHHHNHLPSH 64

Query: 62  FTNPPSTDDVILHACKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQS 121
           FTN PS+++    ACKATRFPDQCGSSLSHS SLPPNPT LQI+QSA+ +SSDGLA+A+S
Sbjct: 65  FTNSPSSEET-FRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAITVSSDGLASAKS 124

Query: 122 MVNQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHY 181
           MV QILDSS  + NRTTAAKNCLEVLR S+YRISLTNDA+RRGRTKDARAW+SASLLYHY
Sbjct: 125 MVKQILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHY 184

Query: 182 DCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFW 241
           DCWSALKYANDTQ V+KTMAFLDSLIGL+SNALSML AYDISGNDTAAWTPPRTERDGFW
Sbjct: 185 DCWSALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTPPRTERDGFW 244

Query: 242 EGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEG 301
           EG  GGSGLGFSGGFPSKV A ATVCKDGAGGCYKTVQEAVNAAP+NA  +RFVIHIKEG
Sbjct: 245 EGSSGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEG 304

Query: 302 VYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCD 361
           VY+EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATV              
Sbjct: 305 VYDEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATV-------------- 364

Query: 362 GSGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY 421
             GV+GDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY
Sbjct: 365 --GVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY 424

Query: 422 KSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQN 481
           KSCKIQGNVDFIFGNSAS+FQDCEILVRPRQLKPEKGENNA+TA GRTDPAQ TGFVFQN
Sbjct: 425 KSCKIQGNVDFIFGNSASVFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQN 484

Query: 482 CLINGTQEYMSLYYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALK 541
           CLINGTQEYM+LYYSKPKVHKN+LGRPWKEYSRTVFIHC LEALV+PAGWMPW+GDFALK
Sbjct: 485 CLINGTQEYMALYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALK 544

Query: 542 TLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQSSNEPKMLN 597
           TL+YGEFENSG GSKTSERV WSSQIPAQHV+SYSVQNFIQGDEWIPSSQSS E K +N
Sbjct: 545 TLYYGEFENSGTGSKTSERVEWSSQIPAQHVYSYSVQNFIQGDEWIPSSQSSEESKTIN 584

BLAST of ClCG02G000597 vs. ExPASy Swiss-Prot
Match: Q9LXD9 (Probable pectinesterase/pectinesterase inhibitor 51 OS=Arabidopsis thaliana OX=3702 GN=PME51 PE=2 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 4.9e-204
Identity = 364/565 (64.42%), Postives = 427/565 (75.58%), Query Frame = 0

Query: 29  SFLSLFFFFLSLSSSSAAARHRHHHL--PTHFTNPPST-DDVILHACKATRFPDQCGSSL 88
           S L L F     SS S+++RH HHH    T   NP S+    I  AC ATR+PDQC SSL
Sbjct: 3   SILILLFSLFLFSSPSSSSRHHHHHNSGDTSPVNPSSSLAAQIRLACNATRYPDQCVSSL 62

Query: 89  SHSSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAKNLNRTTAAKNCLEVLRN 148
           S    +PP+P P+QI+ SA++ S   L TAQS +  I+DSS  NLNRT AA  CL++L  
Sbjct: 63  SEQGRVPPDPKPIQIIHSAISFSFQNLKTAQSKIKSIVDSSVGNLNRTNAANTCLQLLTY 122

Query: 149 SDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGL 208
           S++R   T+ AL RG+ KDARAW+SA+L+Y YD WSALKY NDT  V +TM+FLD LI +
Sbjct: 123 SEHRTQSTDQALTRGKIKDARAWMSAALVYQYDSWSALKYVNDTSQVGETMSFLDGLIHV 182

Query: 209 SSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGGGGGSGL----GFSGGFPSKVAADAT 268
           +SNALSM+V+YD  G++ A+WT P TERDGFWE  G G GL    G + GFPS +  D T
Sbjct: 183 TSNALSMMVSYDNFGDNVASWTYPATERDGFWEKTGPGLGLDPSTGLNLGFPSGLKEDVT 242

Query: 269 VCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGK 328
           VCKDG  G YKTVQ+AVNAAPE+   R+FVI I EGVYEE V VP EKKNVVF+GDGMGK
Sbjct: 243 VCKDGKCG-YKTVQDAVNAAPEDNGMRKFVIKISEGVYEENVIVPFEKKNVVFIGDGMGK 302

Query: 329 TVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCDGSGVLGDGFMASGLTIQNTAGPDA 388
           TVITGSLN G PGI+TYNTATV                GV+GDGFMA  LT QNTAGPDA
Sbjct: 303 TVITGSLNAGMPGITTYNTATV----------------GVVGDGFMARDLTFQNTAGPDA 362

Query: 389 HQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCE 448
           HQAVAFRSDSD S+I++CEFLGNQDTLYAH LRQFYK+C+IQGNVDFIFGNSA++FQDCE
Sbjct: 363 HQAVAFRSDSDFSLIENCEFLGNQDTLYAHGLRQFYKNCRIQGNVDFIFGNSAAVFQDCE 422

Query: 449 ILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQNCLINGTQEYMSLYYSKPKVHKNYL 508
           IL+ PRQ+ PEKGE NA+TAQGR DP+Q+TGFVF NCLINGT+EYM L+ + PKVHKN+L
Sbjct: 423 ILIAPRQINPEKGEKNAVTAQGRIDPSQSTGFVFLNCLINGTEEYMKLFKANPKVHKNFL 482

Query: 509 GRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSS 568
           GRPWK+YSRTVFI C LEAL+TP GW+PWSGDFALKTL+YGE +N+G GS  S+RV WSS
Sbjct: 483 GRPWKDYSRTVFIGCNLEALITPDGWLPWSGDFALKTLYYGESKNTGPGSDRSQRVSWSS 542

Query: 569 QIPAQHVFSYSVQNFIQGDEWIPSS 587
           QIP +HV  YSV NFIQ DEW   S
Sbjct: 543 QIPDEHVHVYSVANFIQADEWASMS 550

BLAST of ClCG02G000597 vs. ExPASy Swiss-Prot
Match: Q8L7Q7 (Probable pectinesterase/pectinesterase inhibitor 64 OS=Arabidopsis thaliana OX=3702 GN=PME64 PE=2 SV=2)

HSP 1 Score: 639.0 bits (1647), Expect = 5.3e-182
Identity = 334/589 (56.71%), Postives = 419/589 (71.14%), Query Frame = 0

Query: 12  ISKSTLLFLSMASLSFLSFLSLFFFFLSLSSSSAAARHRHHHLPTHFT----------NP 71
           +S++ +L +  AS   L  +SL   + ++S SS     R+HH P+H T           P
Sbjct: 31  LSRNGILIIIAASCILLLLISL-LIYATVSKSS-----RNHHNPSHQTPTSDDHPPPETP 90

Query: 72  PSTDDV--ILHACKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQSMV 131
           PS   +  I  AC ATRFPD C +SLS    +PP+P P+QI+ SA+++S + L + QS +
Sbjct: 91  PSPPPIAQIRLACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKI 150

Query: 132 NQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDC 191
             ILDSSA N NRT  A  CLE+L  S +R   T+ A+  G  KDARAW+SA+L Y +DC
Sbjct: 151 QSILDSSAGNRNRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDC 210

Query: 192 WSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEG 251
           WS LK  NDT+ V  T+ F + L+ L+ NALSM++++D  G+D  +W  P TERDGFWE 
Sbjct: 211 WSGLKTVNDTKQVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEK 270

Query: 252 G----GGGSGLGFSGGFPSKVAADATVCKDGAGGC-YKTVQEAVNAAPENAAARRFVIHI 311
                G G+G   S GFPS +  D TVCK+G   C YKTVQEAV++AP+     +FVI I
Sbjct: 271 AGPSLGSGTGTEASLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRI 330

Query: 312 KEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSV 371
           +EGVYEE VRVP EKKNVVF+GDGMGKTVITGSLNVGQPG++T+ +ATV           
Sbjct: 331 REGVYEETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATV----------- 390

Query: 372 RCDGSGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLR 431
                GVLGDGFMA  LTI+NTAG DAHQAVAFRSDSD SV+++CEFLGNQDTLYAHSLR
Sbjct: 391 -----GVLGDGFMARDLTIENTAGADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLR 450

Query: 432 QFYKSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEK-GENNAITAQGRTDPAQATGF 491
           QFYK C+IQGNVDFIFGNSA++FQDC+IL+  +  K E+ G NNAITA GR D +Q+TGF
Sbjct: 451 QFYKQCRIQGNVDFIFGNSAAVFQDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGF 510

Query: 492 VFQNCLINGTQEYMSLYYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGD 551
           VF NC INGT+EYM  + + P+ HKN+LGRPWKE+SRTVF++C LE+L++P GWMPW+GD
Sbjct: 511 VFLNCSINGTEEYMKEFQANPEGHKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGD 570

Query: 552 FALKTLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEW 583
           FALKTL+YGE++N+G GS  S RV WSS+IP +HV  YSV NFIQ DEW
Sbjct: 571 FALKTLYYGEYKNTGPGSVRSSRVPWSSEIPEKHVDVYSVANFIQADEW 597

BLAST of ClCG02G000597 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 2.8e-82
Identity = 200/521 (38.39%), Postives = 284/521 (54.51%), Query Frame = 0

Query: 72  CKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAKNLN 131
           C  T  P  C   L+  + +          + ++ L+ +   TAQS     L S  +N  
Sbjct: 32  CGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLALERATTAQSR-TYTLGSKCRNER 91

Query: 132 RTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQL 191
              A ++C E+   +  +++ T+++       D + WLS +L     C ++L        
Sbjct: 92  EKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLSTALTNLETCRASL-------- 151

Query: 192 VDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGF--WEGGGGGSGLGFS 251
             + +   + ++ L SN ++ L++  +S N      P  + +DGF  W   G    L   
Sbjct: 152 --EDLGVPEYVLPLLSNNVTKLISNTLSLNKVPYNEP--SYKDGFPTWVKPGDRKLL--- 211

Query: 252 GGFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEGVYEEIVRVPLEK 311
               +   A+  V +DG+G   KT+QEAV AA   A   R+VI+IK G Y E + V L  
Sbjct: 212 ---QTTPRANIVVAQDGSGN-VKTIQEAV-AAASRAGGSRYVIYIKAGTYNENIEVKL-- 271

Query: 312 KNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCDGSGVLGDGFMAS 371
           KN++F+GDG+GKT+ITGS +VG  G +T+ +ATV                 V+GD F+A 
Sbjct: 272 KNIMFVGDGIGKTIITGSKSVG-GGATTFKSATV----------------AVVGDNFIAR 331

Query: 372 GLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFI 431
            +TI+NTAGP+ HQAVA RS SDLSV   C F G QDTLY HS RQFY+ C I G VDFI
Sbjct: 332 DITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFI 391

Query: 432 FGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQNCLINGTQEYMSL 491
           FGN+A + Q+C I  R    KP     N +TAQGRTDP Q+TG +  NC +    +   +
Sbjct: 392 FGNAAVVLQNCNIFAR----KP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPV 451

Query: 492 YYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALKTLFYGEFENSGA 551
             S     K +LGRPWK+YSRTV+I   L++L+ PAGWM WSGDFAL TL+Y E+ N+G 
Sbjct: 452 QSSV----KTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGP 502

Query: 552 GSKTSERVGWSS----QIPAQHVFSYSVQNFIQGDEWIPSS 587
           GS T+ RV W        P+Q V  ++V NFI G+ W+P++
Sbjct: 512 GSSTANRVKWRGYHVLTSPSQ-VSQFTVGNFIAGNSWLPAT 502

BLAST of ClCG02G000597 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 303.1 bits (775), Expect = 6.9e-81
Identity = 197/519 (37.96%), Postives = 282/519 (54.34%), Query Frame = 0

Query: 72  CKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAKNLN 131
           C  T  P  C   L+H+S+  P  +  + L+ ++ L  D    A++     L    ++  
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHA-FTLGPKCRDTR 93

Query: 132 RTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQL 191
              A ++C+++   +  +I+ T D   +    DA+ WLS +L     C +       T +
Sbjct: 94  EKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDI 153

Query: 192 VDKTMAFLDSLIGLSSNALSMLVAYDISGNDTA-AWTPPRTERDGFWEGGGGGSGLGFSG 251
           V          + L SN +S L+   ++ N     +TPP  E+DGF      G       
Sbjct: 154 V----------LPLMSNNVSNLLCNTLAINKVPFNYTPP--EKDGFPSWVKPGDRKLLQS 213

Query: 252 GFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEGVYEEIVRVPLEKK 311
             P     +A V KDG+G  +KT++EA++AA   + + RFVI++K+GVY E   + + KK
Sbjct: 214 STPKD---NAVVAKDGSGN-FKTIKEAIDAA---SGSGRFVIYVKQGVYSE--NLEIRKK 273

Query: 312 NVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCDGSGVLGDGFMASG 371
           NV+  GDG+GKT+ITGS +VG  G +T+N+ATV  V                GDGF+A G
Sbjct: 274 NVMLRGDGIGKTIITGSKSVG-GGTTTFNSATVAAV----------------GDGFIARG 333

Query: 372 LTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIF 431
           +T +NTAG    QAVA RS SDLSV   C F   QDTLY HS RQFY+ C + G VDFIF
Sbjct: 334 ITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIF 393

Query: 432 GNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQNCLINGTQEYMSLY 491
           GN+A++ Q+C I  R    +P + + N ITAQGR+DP Q TG +  N  +    +   + 
Sbjct: 394 GNAAAVLQNCNIFAR----RP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVL 453

Query: 492 YSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALKTLFYGEFENSGAG 551
            S     K YLGRPW++YSRTVF+  +L++L+ P GW+ W G+FALKTLFY EF+N+G G
Sbjct: 454 GST----KTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPG 504

Query: 552 SKTSERVGWSS---QIPAQHVFSYSVQNFIQGDEWIPSS 587
           + TS RV W        A     ++V  F+ G  WIPSS
Sbjct: 514 ASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS 504

BLAST of ClCG02G000597 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 299.7 bits (766), Expect = 7.6e-80
Identity = 200/579 (34.54%), Postives = 295/579 (50.95%), Query Frame = 0

Query: 31  LSLFFFFLSLSSSSAAARHRHHHLPTHFTNPPSTDDVILHACKATRFPDQCGSSLSHSSS 90
           +S F   + +S +   ++H       H      T   +   C  T F D C +SL  +S 
Sbjct: 17  VSGFLVIMVVSVAVVTSKHSPKDDENHIR---KTTKAVQAVCAPTDFKDTCVNSLMGASP 76

Query: 91  LPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAKNLNRTTAAKNCLEVLRNS---- 150
              +P  L  L   V + S    + +     I   + KN     A + C +++ ++    
Sbjct: 77  DSDDPVDLIKLGFKVTIKSIN-ESLEKASGDIKAKADKNPEAKGAFELCEKLMIDAIDDL 136

Query: 151 ----DYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSL 210
               D+  S+    +     +D R WLS S+ +   C  +        + D    F  S 
Sbjct: 137 KKCMDHGFSVDQIEV---FVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDMLKIFKTSR 196

Query: 211 IGLSSNALSMLVAY-------DISGNDTAAWTPPR---TERDGF--WEGGGGGSGLGFSG 270
             LSSN+L+M+          +++G   A     R   +  D    W G      +   G
Sbjct: 197 -ELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPTWVGPEARRLMAAQG 256

Query: 271 GFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEGVYEEIVRVPLEKK 330
           G P  V A+A V +DG G  +KT+ +A+NA P+      F+IHIKEG+Y+E V V  +  
Sbjct: 257 GGPGPVKANAVVAQDGTGQ-FKTITDALNAVPKGNKV-PFIIHIKEGIYKEKVTVTKKMP 316

Query: 331 NVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCDGSGVLGDGFMASG 390
           +V F+GDG  KT+ITGSLN G   + T+ TAT+                 + GD F A  
Sbjct: 317 HVTFIGDGPNKTLITGSLNFGIGKVKTFLTATIT----------------IEGDHFTAKN 376

Query: 391 LTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIF 450
           + I+NTAGP+  QAVA R  +D +V   C+  G+QDTLY HS RQFY+ C + G VDFIF
Sbjct: 377 IGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIF 436

Query: 451 GNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQNCLINGTQEYMSLY 510
           G++  I Q+C+I+VR    KP KG+   +TAQGR++  ++TG V   C I G   Y+ + 
Sbjct: 437 GDAKCILQNCKIVVR----KPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPM- 496

Query: 511 YSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALKTLFYGEFENSGAG 570
                V+K YLGRPWKE+SRT+ +  T++ ++ PAGW+PWSGDFALKTL+Y E  N+G G
Sbjct: 497 ---KSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPG 556

Query: 571 SKTSERVGWS--SQIPAQHVFSYSVQNFIQGDEWIPSSQ 588
           S  ++RV W    ++  Q    Y+   F++GD WIP +Q
Sbjct: 557 SNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQ 561

BLAST of ClCG02G000597 vs. ExPASy TrEMBL
Match: A0A1S3CD05 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499643 PE=3 SV=1)

HSP 1 Score: 1059.7 bits (2739), Expect = 4.6e-306
Identity = 541/609 (88.83%), Postives = 563/609 (92.45%), Query Frame = 0

Query: 1   MLPRKPPFPFPISKSTLLFLSMASLSFLSFLSL--FFFFLSLSSSSAAARHR--HHHLPT 60
           + PRKP   FPISKSTLLFLSMASLSFLSFLSL  FFF LSLSSSS+A RH   H HLP+
Sbjct: 2   VFPRKP--TFPISKSTLLFLSMASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLPS 61

Query: 61  HFTNPPSTDDVILHACKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQ 120
           HFTN PS  D ILHACK+TRFPDQCGSSLSHSS  PPNP+PLQILQSA+ALSSDGL TAQ
Sbjct: 62  HFTNTPS--DQILHACKSTRFPDQCGSSLSHSSXSPPNPSPLQILQSAIALSSDGLTTAQ 121

Query: 121 SMVNQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYH 180
           S+V QILDSSA NLNRTTAAKNCLEVLRNSDYRISLTNDAL RGRTKDARAWLSASLLYH
Sbjct: 122 SLVKQILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYH 181

Query: 181 YDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGF 240
           YDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDI GNDTAAWTPPRTERDGF
Sbjct: 182 YDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGF 241

Query: 241 WEGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKE 300
           WEGGGGGSGLGFSGGFP+KV ADATVCKDG+GGCY ++Q+AV+AAPENAAARRFVIHIKE
Sbjct: 242 WEGGGGGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKE 301

Query: 301 GVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRC 360
           GVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLN GQPGISTYNTAT+             
Sbjct: 302 GVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATL------------- 361

Query: 361 DGSGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQF 420
              GVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEF+GNQDTLYAHSLRQF
Sbjct: 362 ---GVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQF 421

Query: 421 YKSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQ 480
           YKSCKIQGNVDFIFGNSASIFQDCEIL+RPRQLKPEKGENNAITA GRTDPAQ+TGFVFQ
Sbjct: 422 YKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQ 481

Query: 481 NCLINGTQEYMSLYYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFAL 540
           NCLINGTQEYMSLYYSKPKVHKN+LGRPWKEYSRTVFIHC LEALV+P+GWMPWSGDFAL
Sbjct: 482 NCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFAL 541

Query: 541 KTLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQSSNEPKMLN 600
           KTLFYGEFENSG GSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSS+SSN+ KMLN
Sbjct: 542 KTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNDQKMLN 590

Query: 601 KKSKDVKLD 606
           KKSKDVKLD
Sbjct: 602 KKSKDVKLD 590

BLAST of ClCG02G000597 vs. ExPASy TrEMBL
Match: A0A0A0LQ80 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G302130 PE=3 SV=1)

HSP 1 Score: 1051.2 bits (2717), Expect = 1.6e-303
Identity = 537/608 (88.32%), Postives = 564/608 (92.76%), Query Frame = 0

Query: 1   MLPRKPPFPFPISKSTLLFLSMASLSFLSFLSLFFFFLSLSSSSAAARHRHH---HLPTH 60
           + PRKP FPF  SKSTLLFLSMASLSFLSFLSLFFFF SLS SS++A H HH   HLP+H
Sbjct: 2   VFPRKPTFPF--SKSTLLFLSMASLSFLSFLSLFFFFSSLSLSSSSADHHHHPHPHLPSH 61

Query: 61  FTNPPSTDDVILHACKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQS 120
           FTN PS  D ILHACK+TRFPDQCGSSLSH SSLPPNP+PLQILQSA+ALSSDGL TAQS
Sbjct: 62  FTNYPS--DQILHACKSTRFPDQCGSSLSH-SSLPPNPSPLQILQSAIALSSDGLTTAQS 121

Query: 121 MVNQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHY 180
           +V QILDSSA NLNRTTAAKNCLEVLRNSDYRISLTNDAL  GRTKDARAWLSASLLYHY
Sbjct: 122 LVKQILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHHGRTKDARAWLSASLLYHY 181

Query: 181 DCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFW 240
           DCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDI GNDTAAWTPPRTERDGFW
Sbjct: 182 DCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFW 241

Query: 241 EGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEG 300
           E GGGGSGLGFSGGFP+KV ADATVCKDG+GGCY ++Q+AV+AAPENAAARRFVIHIKEG
Sbjct: 242 E-GGGGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEG 301

Query: 301 VYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCD 360
           VYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPG+STYNTAT+              
Sbjct: 302 VYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATL-------------- 361

Query: 361 GSGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY 420
             GV+GDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY
Sbjct: 362 --GVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY 421

Query: 421 KSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQN 480
           KSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNA+TA GRTDPAQ+TGFVFQN
Sbjct: 422 KSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQSTGFVFQN 481

Query: 481 CLINGTQEYMSLYYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALK 540
           CLINGTQEYMSLYYSKP+VHKN+LGRPWKEYSRTVFIHC LEALV+P+GWMPWSGDFALK
Sbjct: 482 CLINGTQEYMSLYYSKPQVHKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALK 541

Query: 541 TLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQSSNEPKMLNK 600
           TLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSV+NFIQGDEWIPSS+SS+E KMLNK
Sbjct: 542 TLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVENFIQGDEWIPSSESSDEQKMLNK 587

Query: 601 KSKDVKLD 606
           +SKDVKLD
Sbjct: 602 RSKDVKLD 587

BLAST of ClCG02G000597 vs. ExPASy TrEMBL
Match: A0A6J1EV58 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438335 PE=3 SV=1)

HSP 1 Score: 1006.5 bits (2601), Expect = 4.7e-290
Identity = 509/599 (84.97%), Postives = 541/599 (90.32%), Query Frame = 0

Query: 2   LPRKPPFPFPI----SKSTLLFLSMASLSFLSFLSLFFFFLSLSSSSAAARHRHHHLPTH 61
           LPR  PFPFP     +KST LFLSMA+LSFLSFLSL FFFLSL SS+   RH HHHLP+H
Sbjct: 5   LPRTTPFPFPFQFNAAKSTFLFLSMAALSFLSFLSL-FFFLSL-SSAVHHRHHHHHLPSH 64

Query: 62  FTNPPSTDDVILHACKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQS 121
           FTN PS+++    ACKATRFPDQCGSSLSHS SLPPNPT LQI+QSA+A+SSDGLA+A+S
Sbjct: 65  FTNSPSSEET-FRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKS 124

Query: 122 MVNQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHY 181
           MV QILDSS  + NRTTAAKNCLEVLR S+YRISLTNDA+RRGRTKDARAW+SASLLYHY
Sbjct: 125 MVKQILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHY 184

Query: 182 DCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFW 241
           DCWSALKYANDTQ V+KTMAFLDSLIGL+SNALSML AYDISGNDTAAWTPPRTERDGFW
Sbjct: 185 DCWSALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTPPRTERDGFW 244

Query: 242 EGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEG 301
           EG  GGSGLGFSGGFPSKV A ATVCKDGAGGCYKTVQEAVNAAP+NA  +RFVIHIKEG
Sbjct: 245 EGSSGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEG 304

Query: 302 VYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCD 361
           VY+EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATV              
Sbjct: 305 VYDEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATV-------------- 364

Query: 362 GSGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY 421
             GV+GDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY
Sbjct: 365 --GVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY 424

Query: 422 KSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQN 481
           KSCKIQGNVDFIFGNSAS+FQDCEILVRPRQLKPEKGENNA+TA GRTDPAQ TGFVFQN
Sbjct: 425 KSCKIQGNVDFIFGNSASVFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQN 484

Query: 482 CLINGTQEYMSLYYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALK 541
           CL+NGTQEYM+LYYSKPKVHKN+LGRPWKEYSRTVFIHC LEALV+PAGWMPW+GDFALK
Sbjct: 485 CLVNGTQEYMALYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALK 544

Query: 542 TLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQSSNEPKMLN 597
           TL+YGEFENSG GSKTSERV WSSQIPAQHV+SYSVQNFIQGDEWIPSSQSS E K +N
Sbjct: 545 TLYYGEFENSGTGSKTSERVEWSSQIPAQHVYSYSVQNFIQGDEWIPSSQSSEESKTIN 584

BLAST of ClCG02G000597 vs. ExPASy TrEMBL
Match: A0A6J1I3S8 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470304 PE=3 SV=1)

HSP 1 Score: 992.6 bits (2565), Expect = 7.0e-286
Identity = 505/599 (84.31%), Postives = 538/599 (89.82%), Query Frame = 0

Query: 2   LPRKPPFP----FPISKSTLLFLSMASLSFLSFLSLFFFFLSLSSSSAAARHRHHHLPTH 61
           LPR  PFP    F  +KST LFLSMA+LSFLSFL L FFFLSLSS   A  HRHHHLP+H
Sbjct: 5   LPRTTPFPFLFQFTTAKSTFLFLSMAALSFLSFLCL-FFFLSLSS---AVHHRHHHLPSH 64

Query: 62  FTNPPSTDDVILHACKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQS 121
           FTN PS+++  L ACKATRFP+QCGSSLSHS SLPPNPT LQI+QSA+A+SSDGLA+A+S
Sbjct: 65  FTNFPSSEET-LRACKATRFPNQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKS 124

Query: 122 MVNQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHY 181
           MV +ILDSS  + NRTTAAKNCLEVLR S+YRISLTNDA+RRGRTKDARAW+SASLLYHY
Sbjct: 125 MVKKILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHY 184

Query: 182 DCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFW 241
           DCWSALKYANDTQ V+KTMAFLDSLIGL+SNALSML AYDISGNDTAAWTPPRTERDGFW
Sbjct: 185 DCWSALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTPPRTERDGFW 244

Query: 242 EGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEG 301
           EG  GGSGLGFSGGFPSKV A ATVCKDGAGGCYKTVQEAVNAAP+NA  +RFVIHIKEG
Sbjct: 245 EGSSGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEG 304

Query: 302 VYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCD 361
           VY+EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYN+ATV              
Sbjct: 305 VYDEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNSATV-------------- 364

Query: 362 GSGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY 421
             GV+GDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY
Sbjct: 365 --GVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY 424

Query: 422 KSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQN 481
           KSCKIQGNVDFIFGNSAS+FQDCEILV PRQLKPEKGENNA+TA GRTDPAQ TGFVFQN
Sbjct: 425 KSCKIQGNVDFIFGNSASVFQDCEILVCPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQN 484

Query: 482 CLINGTQEYMSLYYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALK 541
           CLINGTQEYM+LYYSKPKVHKN+LGRPWKEYSRTVFIHC LEALV+PAGWMPW+GDFALK
Sbjct: 485 CLINGTQEYMALYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALK 544

Query: 542 TLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQSSNEPKMLN 597
           TL+YGEFENSG GS TSERV WSSQIPAQHV+SYSVQNFIQGDEWIPSSQSS E K +N
Sbjct: 545 TLYYGEFENSGTGSITSERVEWSSQIPAQHVYSYSVQNFIQGDEWIPSSQSSEESKTIN 582

BLAST of ClCG02G000597 vs. ExPASy TrEMBL
Match: A0A5A7TBR9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00730 PE=3 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 1.7e-284
Identity = 496/562 (88.26%), Postives = 516/562 (91.81%), Query Frame = 0

Query: 48  RHRHHHLPTHFTNPPSTDDV----ILHACKATRFPDQCGSSLSHSSSLPPNPTPLQILQS 107
           RHR    P   +  P    V    ILHACK+TRFPDQCGSSLSH SSLPPNP+PLQILQS
Sbjct: 18  RHRLPRSPLQPSQQPPVTFVFSFPILHACKSTRFPDQCGSSLSH-SSLPPNPSPLQILQS 77

Query: 108 AVALSSDGLATAQSMVNQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTK 167
           A+ALSSDGL TAQS+V QILDSSA NLNRTTAAKNCLEVLRNSDYRISLTNDAL RGRTK
Sbjct: 78  AIALSSDGLTTAQSLVKQILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTK 137

Query: 168 DARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDT 227
           DARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDI GNDT
Sbjct: 138 DARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDT 197

Query: 228 AAWTPPRTERDGFWEGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYKTVQEAVNAAPE 287
           AAWTPPRTERDGFWEGGGGGSGLGFSGGFP+KV ADATVCKDG+GGCY ++Q+AV+AAPE
Sbjct: 198 AAWTPPRTERDGFWEGGGGGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPE 257

Query: 288 NAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATV 347
           NAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLN GQPGISTYNTAT+
Sbjct: 258 NAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATL 317

Query: 348 EEVWMTRILSVRCDGSGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLG 407
                           GVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEF+G
Sbjct: 318 ----------------GVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIG 377

Query: 408 NQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQG 467
           NQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEIL+RPRQLKPEKGENNAITA G
Sbjct: 378 NQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILIRPRQLKPEKGENNAITAHG 437

Query: 468 RTDPAQATGFVFQNCLINGTQEYMSLYYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVT 527
           RTDPAQ+TGFVFQNCLINGTQEYMSLYYSKPKVHKN+LGRPWKEYSRTVFIHC LEALV+
Sbjct: 438 RTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVS 497

Query: 528 PAGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWI 587
           P+GWMPWSGDFALKTLFYGEFENSG GSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWI
Sbjct: 498 PSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWI 557

Query: 588 PSSQSSNEPKMLNKKSKDVKLD 606
           PSS+SSN+ KMLNKKSKDVKLD
Sbjct: 558 PSSESSNDQKMLNKKSKDVKLD 562

BLAST of ClCG02G000597 vs. TAIR 10
Match: AT5G09760.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 712.2 bits (1837), Expect = 3.5e-205
Identity = 364/565 (64.42%), Postives = 427/565 (75.58%), Query Frame = 0

Query: 29  SFLSLFFFFLSLSSSSAAARHRHHHL--PTHFTNPPST-DDVILHACKATRFPDQCGSSL 88
           S L L F     SS S+++RH HHH    T   NP S+    I  AC ATR+PDQC SSL
Sbjct: 3   SILILLFSLFLFSSPSSSSRHHHHHNSGDTSPVNPSSSLAAQIRLACNATRYPDQCVSSL 62

Query: 89  SHSSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAKNLNRTTAAKNCLEVLRN 148
           S    +PP+P P+QI+ SA++ S   L TAQS +  I+DSS  NLNRT AA  CL++L  
Sbjct: 63  SEQGRVPPDPKPIQIIHSAISFSFQNLKTAQSKIKSIVDSSVGNLNRTNAANTCLQLLTY 122

Query: 149 SDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGL 208
           S++R   T+ AL RG+ KDARAW+SA+L+Y YD WSALKY NDT  V +TM+FLD LI +
Sbjct: 123 SEHRTQSTDQALTRGKIKDARAWMSAALVYQYDSWSALKYVNDTSQVGETMSFLDGLIHV 182

Query: 209 SSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGGGGGSGL----GFSGGFPSKVAADAT 268
           +SNALSM+V+YD  G++ A+WT P TERDGFWE  G G GL    G + GFPS +  D T
Sbjct: 183 TSNALSMMVSYDNFGDNVASWTYPATERDGFWEKTGPGLGLDPSTGLNLGFPSGLKEDVT 242

Query: 269 VCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGK 328
           VCKDG  G YKTVQ+AVNAAPE+   R+FVI I EGVYEE V VP EKKNVVF+GDGMGK
Sbjct: 243 VCKDGKCG-YKTVQDAVNAAPEDNGMRKFVIKISEGVYEENVIVPFEKKNVVFIGDGMGK 302

Query: 329 TVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCDGSGVLGDGFMASGLTIQNTAGPDA 388
           TVITGSLN G PGI+TYNTATV                GV+GDGFMA  LT QNTAGPDA
Sbjct: 303 TVITGSLNAGMPGITTYNTATV----------------GVVGDGFMARDLTFQNTAGPDA 362

Query: 389 HQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCE 448
           HQAVAFRSDSD S+I++CEFLGNQDTLYAH LRQFYK+C+IQGNVDFIFGNSA++FQDCE
Sbjct: 363 HQAVAFRSDSDFSLIENCEFLGNQDTLYAHGLRQFYKNCRIQGNVDFIFGNSAAVFQDCE 422

Query: 449 ILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQNCLINGTQEYMSLYYSKPKVHKNYL 508
           IL+ PRQ+ PEKGE NA+TAQGR DP+Q+TGFVF NCLINGT+EYM L+ + PKVHKN+L
Sbjct: 423 ILIAPRQINPEKGEKNAVTAQGRIDPSQSTGFVFLNCLINGTEEYMKLFKANPKVHKNFL 482

Query: 509 GRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSS 568
           GRPWK+YSRTVFI C LEAL+TP GW+PWSGDFALKTL+YGE +N+G GS  S+RV WSS
Sbjct: 483 GRPWKDYSRTVFIGCNLEALITPDGWLPWSGDFALKTLYYGESKNTGPGSDRSQRVSWSS 542

Query: 569 QIPAQHVFSYSVQNFIQGDEWIPSS 587
           QIP +HV  YSV NFIQ DEW   S
Sbjct: 543 QIPDEHVHVYSVANFIQADEWASMS 550

BLAST of ClCG02G000597 vs. TAIR 10
Match: AT5G64640.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 639.0 bits (1647), Expect = 3.8e-183
Identity = 334/589 (56.71%), Postives = 419/589 (71.14%), Query Frame = 0

Query: 12  ISKSTLLFLSMASLSFLSFLSLFFFFLSLSSSSAAARHRHHHLPTHFT----------NP 71
           +S++ +L +  AS   L  +SL   + ++S SS     R+HH P+H T           P
Sbjct: 31  LSRNGILIIIAASCILLLLISL-LIYATVSKSS-----RNHHNPSHQTPTSDDHPPPETP 90

Query: 72  PSTDDV--ILHACKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQSMV 131
           PS   +  I  AC ATRFPD C +SLS    +PP+P P+QI+ SA+++S + L + QS +
Sbjct: 91  PSPPPIAQIRLACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKI 150

Query: 132 NQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDC 191
             ILDSSA N NRT  A  CLE+L  S +R   T+ A+  G  KDARAW+SA+L Y +DC
Sbjct: 151 QSILDSSAGNRNRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDC 210

Query: 192 WSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEG 251
           WS LK  NDT+ V  T+ F + L+ L+ NALSM++++D  G+D  +W  P TERDGFWE 
Sbjct: 211 WSGLKTVNDTKQVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEK 270

Query: 252 G----GGGSGLGFSGGFPSKVAADATVCKDGAGGC-YKTVQEAVNAAPENAAARRFVIHI 311
                G G+G   S GFPS +  D TVCK+G   C YKTVQEAV++AP+     +FVI I
Sbjct: 271 AGPSLGSGTGTEASLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRI 330

Query: 312 KEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSV 371
           +EGVYEE VRVP EKKNVVF+GDGMGKTVITGSLNVGQPG++T+ +ATV           
Sbjct: 331 REGVYEETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATV----------- 390

Query: 372 RCDGSGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLR 431
                GVLGDGFMA  LTI+NTAG DAHQAVAFRSDSD SV+++CEFLGNQDTLYAHSLR
Sbjct: 391 -----GVLGDGFMARDLTIENTAGADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLR 450

Query: 432 QFYKSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEK-GENNAITAQGRTDPAQATGF 491
           QFYK C+IQGNVDFIFGNSA++FQDC+IL+  +  K E+ G NNAITA GR D +Q+TGF
Sbjct: 451 QFYKQCRIQGNVDFIFGNSAAVFQDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGF 510

Query: 492 VFQNCLINGTQEYMSLYYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGD 551
           VF NC INGT+EYM  + + P+ HKN+LGRPWKE+SRTVF++C LE+L++P GWMPW+GD
Sbjct: 511 VFLNCSINGTEEYMKEFQANPEGHKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGD 570

Query: 552 FALKTLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEW 583
           FALKTL+YGE++N+G GS  S RV WSS+IP +HV  YSV NFIQ DEW
Sbjct: 571 FALKTLYYGEYKNTGPGSVRSSRVPWSSEIPEKHVDVYSVANFIQADEW 597

BLAST of ClCG02G000597 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 303.1 bits (775), Expect = 4.9e-82
Identity = 197/519 (37.96%), Postives = 282/519 (54.34%), Query Frame = 0

Query: 72  CKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAKNLN 131
           C  T  P  C   L+H+S+  P  +  + L+ ++ L  D    A++     L    ++  
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHA-FTLGPKCRDTR 93

Query: 132 RTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQL 191
              A ++C+++   +  +I+ T D   +    DA+ WLS +L     C +       T +
Sbjct: 94  EKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDI 153

Query: 192 VDKTMAFLDSLIGLSSNALSMLVAYDISGNDTA-AWTPPRTERDGFWEGGGGGSGLGFSG 251
           V          + L SN +S L+   ++ N     +TPP  E+DGF      G       
Sbjct: 154 V----------LPLMSNNVSNLLCNTLAINKVPFNYTPP--EKDGFPSWVKPGDRKLLQS 213

Query: 252 GFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEGVYEEIVRVPLEKK 311
             P     +A V KDG+G  +KT++EA++AA   + + RFVI++K+GVY E   + + KK
Sbjct: 214 STPKD---NAVVAKDGSGN-FKTIKEAIDAA---SGSGRFVIYVKQGVYSE--NLEIRKK 273

Query: 312 NVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCDGSGVLGDGFMASG 371
           NV+  GDG+GKT+ITGS +VG  G +T+N+ATV  V                GDGF+A G
Sbjct: 274 NVMLRGDGIGKTIITGSKSVG-GGTTTFNSATVAAV----------------GDGFIARG 333

Query: 372 LTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIF 431
           +T +NTAG    QAVA RS SDLSV   C F   QDTLY HS RQFY+ C + G VDFIF
Sbjct: 334 ITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIF 393

Query: 432 GNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQNCLINGTQEYMSLY 491
           GN+A++ Q+C I  R    +P + + N ITAQGR+DP Q TG +  N  +    +   + 
Sbjct: 394 GNAAAVLQNCNIFAR----RP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVL 453

Query: 492 YSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALKTLFYGEFENSGAG 551
            S     K YLGRPW++YSRTVF+  +L++L+ P GW+ W G+FALKTLFY EF+N+G G
Sbjct: 454 GST----KTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPG 504

Query: 552 SKTSERVGWSS---QIPAQHVFSYSVQNFIQGDEWIPSS 587
           + TS RV W        A     ++V  F+ G  WIPSS
Sbjct: 514 ASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS 504

BLAST of ClCG02G000597 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 299.7 bits (766), Expect = 5.4e-81
Identity = 200/579 (34.54%), Postives = 295/579 (50.95%), Query Frame = 0

Query: 31  LSLFFFFLSLSSSSAAARHRHHHLPTHFTNPPSTDDVILHACKATRFPDQCGSSLSHSSS 90
           +S F   + +S +   ++H       H      T   +   C  T F D C +SL  +S 
Sbjct: 17  VSGFLVIMVVSVAVVTSKHSPKDDENHIR---KTTKAVQAVCAPTDFKDTCVNSLMGASP 76

Query: 91  LPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAKNLNRTTAAKNCLEVLRNS---- 150
              +P  L  L   V + S    + +     I   + KN     A + C +++ ++    
Sbjct: 77  DSDDPVDLIKLGFKVTIKSIN-ESLEKASGDIKAKADKNPEAKGAFELCEKLMIDAIDDL 136

Query: 151 ----DYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSL 210
               D+  S+    +     +D R WLS S+ +   C  +        + D    F  S 
Sbjct: 137 KKCMDHGFSVDQIEV---FVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDMLKIFKTSR 196

Query: 211 IGLSSNALSMLVAY-------DISGNDTAAWTPPR---TERDGF--WEGGGGGSGLGFSG 270
             LSSN+L+M+          +++G   A     R   +  D    W G      +   G
Sbjct: 197 -ELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPTWVGPEARRLMAAQG 256

Query: 271 GFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVIHIKEGVYEEIVRVPLEKK 330
           G P  V A+A V +DG G  +KT+ +A+NA P+      F+IHIKEG+Y+E V V  +  
Sbjct: 257 GGPGPVKANAVVAQDGTGQ-FKTITDALNAVPKGNKV-PFIIHIKEGIYKEKVTVTKKMP 316

Query: 331 NVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRILSVRCDGSGVLGDGFMASG 390
           +V F+GDG  KT+ITGSLN G   + T+ TAT+                 + GD F A  
Sbjct: 317 HVTFIGDGPNKTLITGSLNFGIGKVKTFLTATIT----------------IEGDHFTAKN 376

Query: 391 LTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIF 450
           + I+NTAGP+  QAVA R  +D +V   C+  G+QDTLY HS RQFY+ C + G VDFIF
Sbjct: 377 IGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIF 436

Query: 451 GNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATGFVFQNCLINGTQEYMSLY 510
           G++  I Q+C+I+VR    KP KG+   +TAQGR++  ++TG V   C I G   Y+ + 
Sbjct: 437 GDAKCILQNCKIVVR----KPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPM- 496

Query: 511 YSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSGDFALKTLFYGEFENSGAG 570
                V+K YLGRPWKE+SRT+ +  T++ ++ PAGW+PWSGDFALKTL+Y E  N+G G
Sbjct: 497 ---KSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPG 556

Query: 571 SKTSERVGWS--SQIPAQHVFSYSVQNFIQGDEWIPSSQ 588
           S  ++RV W    ++  Q    Y+   F++GD WIP +Q
Sbjct: 557 SNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQ 561

BLAST of ClCG02G000597 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 299.7 bits (766), Expect = 5.4e-81
Identity = 194/537 (36.13%), Postives = 285/537 (53.07%), Query Frame = 0

Query: 72  CKATRFPDQCGSSLSHSSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAKNLN 131
           C  T + + C +SL  +S  P +  PL +++    ++   +  +    +  L + A N  
Sbjct: 59  CAPTDYKETCVNSLMKAS--PDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDK 118

Query: 132 RTTAAKNCLEVLRN---SDYRISLTN-DALR----RGRTKDARAWLSASLLYHYDCWSAL 191
            T  A    E L N    D +  L N D           +D R WLS S+ Y   C    
Sbjct: 119 DTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTF 178

Query: 192 -----KYANDTQLVDKTMAFLDSL-IGLSSNALSMLVAYDISG--NDTAAWTPP-RTERD 251
                K + D Q + KT   L S  + + +N  ++L  ++++G   D   +     +  D
Sbjct: 179 EETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAED 238

Query: 252 GF--WEGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYKTVQEAVNAAPENAAARRFVI 311
           G   W G      +   GG    V A+  V  DG+G  YKT+ EA+NA P+ A  + FVI
Sbjct: 239 GIPSWVGPNTRRLMATKGG----VKANVVVAHDGSGQ-YKTINEALNAVPK-ANQKPFVI 298

Query: 312 HIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVEEVWMTRIL 371
           +IK+GVY E V V  +  +V F+GDG  KT ITGSLN     + TY TATV         
Sbjct: 299 YIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATV--------- 358

Query: 372 SVRCDGSGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHS 431
                   + GD F A  +  +NTAGP+ HQAVA R  +DL+V  +C+  G QDTLY HS
Sbjct: 359 -------AINGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHS 418

Query: 432 LRQFYKSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQATG 491
            RQF++ C + G VDFIFG+   + Q+C I+VR    KP K ++  ITAQGR+D  ++TG
Sbjct: 419 HRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVR----KPMKSQSCMITAQGRSDKRESTG 478

Query: 492 FVFQNCLINGTQEYMSLYYSKPKVHKNYLGRPWKEYSRTVFIHCTLEALVTPAGWMPWSG 551
            V QNC I G   Y+ +      ++K YLGRPWKE+SRT+ +  T++ ++ PAGW+PW+G
Sbjct: 479 LVLQNCHITGEPAYIPV----KSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNG 538

Query: 552 DFALKTLFYGEFENSGAGSKTSERVGWS--SQIPAQHVFSYSVQNFIQGDEWIPSSQ 588
           DFAL TL+Y E+EN+G GS  ++RV W    ++  +    ++   F++G+ WIP ++
Sbjct: 539 DFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNR 563

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902126.10.0e+0092.40probable pectinesterase/pectinesterase inhibitor 51 [Benincasa hispida][more]
XP_008460902.19.6e-30688.83PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor... [more]
XP_004147375.23.4e-30388.32probable pectinesterase/pectinesterase inhibitor 51 [Cucumis sativus] >KGN62146.... [more]
XP_022932006.19.6e-29084.97probable pectinesterase/pectinesterase inhibitor 51 [Cucurbita moschata][more]
KAG6571352.11.2e-28784.64putative pectinesterase/pectinesterase inhibitor 51, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
Q9LXD94.9e-20464.42Probable pectinesterase/pectinesterase inhibitor 51 OS=Arabidopsis thaliana OX=3... [more]
Q8L7Q75.3e-18256.71Probable pectinesterase/pectinesterase inhibitor 64 OS=Arabidopsis thaliana OX=3... [more]
O048872.8e-8238.39Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
O221496.9e-8137.96Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3... [more]
Q8GXA17.6e-8034.54Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A1S3CD054.6e-30688.83Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499643 PE=3 SV=1[more]
A0A0A0LQ801.6e-30388.32Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G302130 PE=3 SV=1[more]
A0A6J1EV584.7e-29084.97Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438335 PE=3 SV=1[more]
A0A6J1I3S87.0e-28684.31Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470304 PE=3 SV=1[more]
A0A5A7TBR91.7e-28488.26Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00730... [more]
Match NameE-valueIdentityDescription
AT5G09760.13.5e-20564.42Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G64640.13.8e-18356.71Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G45220.14.9e-8237.96Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G06830.15.4e-8134.54Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G49180.15.4e-8136.13Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 63..212
e-value: 6.2E-17
score: 72.3
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 31..212
e-value: 1.1E-11
score: 42.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 68..212
e-value: 5.6E-17
score: 62.5
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 256..595
e-value: 1.1E-109
score: 368.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 64..215
e-value: 4.2E-18
score: 67.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 66..212
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 259..580
e-value: 2.9E-117
score: 390.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 583..605
NoneNo IPR availablePANTHERPTHR31707:SF340PECTINESTERASEcoord: 18..586
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 18..586
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 71..212
e-value: 5.03663E-19
score: 82.1009
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 256..585

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG02G000597.1ClCG02G000597.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity