ClCG01G024630 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG01G024630
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Descriptiongibberellin 2-beta-dioxygenase-like
LocationCG_Chr01: 37643496 .. 37644628 (+)
RNA-Seq ExpressionClCG01G024630
SyntenyClCG01G024630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGTTCTGTCTCATCCAACATTGGATTTGAACCAATATTCTCTTCTCCGATCAACATGCAAACCCACCGCCTCCTTCGCTGAAATTCCTGAAATTGACCTCTCAGATCCAAACGCCAAGTCCAATATCGTCAAAGCTTGCGAAGAGTTTGGCTTCTTCAAGCTCGTAAACCACCGTGTTCCCATCGAGCTTATGACCAAACTTGAAGATGAATCCCTCTGTTTTTTCAAACTTTCGGAATCTGAGAAAGATAAAGCGCGCTCTCCTCACCCTTTAGGCTATGGCTGTAAAAATATCGGCTCTAGTGGCGATAAGGGTTGGATTGAATATCTTCTCCTTAACGCTAATCCTCTCCCCATTTTCTCCCAAAACTCCCATACTTTCCTGTAAGTTGCAAGAAAAAACAGAGCCACTCTCTATAAGTTTTCAATAATATTTGATAAAAACAGAACACCGTTCTTTTGCTGTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAATAATAATTTGTGCGGATGTTGTTGCAGCTGTGCGGCCGAAGAGTATGTGACGGCGGTGAAGAAACTGAGCTCCCAAGTGGTGGAATTAATAGCAGAAGGATTAAAAATGGAGCCAAGAAACGCCATAAGCAAGCTTCTAAACGACGAGAAAGCTGACTGTTGTTTCAGAGTGAATCACTACCCGCCATGTCCAGAACCGCAAGCTTTGGGTGGACGAAACATGATTGGATTCGGTGAACACACAGACCCACAAATCATATCAGTTTTAAGATCAAACAACTCAACCGGCTTACAAATCTGCCTCAGAGACGGCGCGTGGGTTTCAGTGCCGCCTGACGCCGGCGCCTTCTTTGTGAATGTCGGCGACGCGCTGCAGGTGATGACTAACGGCAGATTCAAAAGCGTGAAACATAAAGTGTTGGCGGATCCGAACAGAGAGAGAGTTTCGATGATTTACTTTGGGGGGCCGCCGTTGAGTGAAAAGATTGCGCCATTGCCGCAGCTTTTAGAAGAGGGAGAAGAAAGCTTGTACAAGGAATTCACCTGGTGGGAATACAAAACGGCGGCGTACAAGACCAAATTGGCTGATCATAGGCTTGGTGCCTTTGAAAAGAGTCCAAACTCTTAA

mRNA sequence

ATGGTTGTTCTGTCTCATCCAACATTGGATTTGAACCAATATTCTCTTCTCCGATCAACATGCAAACCCACCGCCTCCTTCGCTGAAATTCCTGAAATTGACCTCTCAGATCCAAACGCCAAGTCCAATATCGTCAAAGCTTGCGAAGAGTTTGGCTTCTTCAAGCTCGTAAACCACCGTGTTCCCATCGAGCTTATGACCAAACTTGAAGATGAATCCCTCTGTTTTTTCAAACTTTCGGAATCTGAGAAAGATAAAGCGCGCTCTCCTCACCCTTTAGGCTATGGCTGTAAAAATATCGGCTCTAGTGGCGATAAGGGTTGGATTGAATATCTTCTCCTTAACGCTAATCCTCTCCCCATTTTCTCCCAAAACTCCCATACTTTCCTCTGTGCGGCCGAAGAGTATGTGACGGCGGTGAAGAAACTGAGCTCCCAAGTGGTGGAATTAATAGCAGAAGGATTAAAAATGGAGCCAAGAAACGCCATAAGCAAGCTTCTAAACGACGAGAAAGCTGACTGTTGTTTCAGAGTGAATCACTACCCGCCATGTCCAGAACCGCAAGCTTTGGGTGGACGAAACATGATTGGATTCGGTGAACACACAGACCCACAAATCATATCAGTTTTAAGATCAAACAACTCAACCGGCTTACAAATCTGCCTCAGAGACGGCGCGTGGGTTTCAGTGCCGCCTGACGCCGGCGCCTTCTTTGTGAATGTCGGCGACGCGCTGCAGGTGATGACTAACGGCAGATTCAAAAGCGTGAAACATAAAGTGTTGGCGGATCCGAACAGAGAGAGAGTTTCGATGATTTACTTTGGGGGGCCGCCGTTGAGTGAAAAGATTGCGCCATTGCCGCAGCTTTTAGAAGAGGGAGAAGAAAGCTTGTACAAGGAATTCACCTGGTGGGAATACAAAACGGCGGCGTACAAGACCAAATTGGCTGATCATAGGCTTGGTGCCTTTGAAAAGAGTCCAAACTCTTAA

Coding sequence (CDS)

ATGGTTGTTCTGTCTCATCCAACATTGGATTTGAACCAATATTCTCTTCTCCGATCAACATGCAAACCCACCGCCTCCTTCGCTGAAATTCCTGAAATTGACCTCTCAGATCCAAACGCCAAGTCCAATATCGTCAAAGCTTGCGAAGAGTTTGGCTTCTTCAAGCTCGTAAACCACCGTGTTCCCATCGAGCTTATGACCAAACTTGAAGATGAATCCCTCTGTTTTTTCAAACTTTCGGAATCTGAGAAAGATAAAGCGCGCTCTCCTCACCCTTTAGGCTATGGCTGTAAAAATATCGGCTCTAGTGGCGATAAGGGTTGGATTGAATATCTTCTCCTTAACGCTAATCCTCTCCCCATTTTCTCCCAAAACTCCCATACTTTCCTCTGTGCGGCCGAAGAGTATGTGACGGCGGTGAAGAAACTGAGCTCCCAAGTGGTGGAATTAATAGCAGAAGGATTAAAAATGGAGCCAAGAAACGCCATAAGCAAGCTTCTAAACGACGAGAAAGCTGACTGTTGTTTCAGAGTGAATCACTACCCGCCATGTCCAGAACCGCAAGCTTTGGGTGGACGAAACATGATTGGATTCGGTGAACACACAGACCCACAAATCATATCAGTTTTAAGATCAAACAACTCAACCGGCTTACAAATCTGCCTCAGAGACGGCGCGTGGGTTTCAGTGCCGCCTGACGCCGGCGCCTTCTTTGTGAATGTCGGCGACGCGCTGCAGGTGATGACTAACGGCAGATTCAAAAGCGTGAAACATAAAGTGTTGGCGGATCCGAACAGAGAGAGAGTTTCGATGATTTACTTTGGGGGGCCGCCGTTGAGTGAAAAGATTGCGCCATTGCCGCAGCTTTTAGAAGAGGGAGAAGAAAGCTTGTACAAGGAATTCACCTGGTGGGAATACAAAACGGCGGCGTACAAGACCAAATTGGCTGATCATAGGCTTGGTGCCTTTGAAAAGAGTCCAAACTCTTAA

Protein sequence

MVVLSHPTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNHRVPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANPLPIFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFRVNHYPPCPEPQALGGRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAFFVNVGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESLYKEFTWWEYKTAAYKTKLADHRLGAFEKSPNS
Homology
BLAST of ClCG01G024630 vs. NCBI nr
Match: XP_038883200.1 (gibberellin 2-beta-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 641.3 bits (1653), Expect = 4.4e-180
Identity = 309/329 (93.92%), Postives = 319/329 (96.96%), Query Frame = 0

Query: 1   MVVLSHPTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNHR 60
           MVVLS PTLDLNQYSLLRSTCKPTASFA+IPEIDLSDPNAKS+I+KACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANPLP 120
           VP+ELMTKLEDESLCFFKLSESEKDKAR PHPLGYGCKNIGS+GDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFRVNH 180
           I SQNS  FLCAAEEYV AVKKLSS+VVELIAEGLKMEPRN ISKLLNDEKADCCFRVNH
Sbjct: 121 ILSQNS--FLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPEPQALGGRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAFFVN 240
           YPPCPE Q+LGGRNMIGFGEHTDPQIISVLRSNNS+GLQICLRDGAWVSVPPDAGA FVN
Sbjct: 181 YPPCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKV+ADPNRERVSMIYFGGPPLSEKI PLPQLLEEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKE 300

Query: 301 FTWWEYKTAAYKTKLADHRLGAFEKSPNS 330
           FTWWEYKTAAYKTKLAD+RLGAFEKSPNS
Sbjct: 301 FTWWEYKTAAYKTKLADYRLGAFEKSPNS 327

BLAST of ClCG01G024630 vs. NCBI nr
Match: XP_008439633.1 (PREDICTED: gibberellin 2-beta-dioxygenase-like [Cucumis melo])

HSP 1 Score: 617.5 bits (1591), Expect = 6.8e-173
Identity = 299/329 (90.88%), Postives = 310/329 (94.22%), Query Frame = 0

Query: 1   MVVLSHPTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNHR 60
           MVVLS PTLDLNQYSLLRSTCKPTASFA+IPEIDLSDPNAK +IVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANPLP 120
           VPIELMTKLEDESLCFFKLSESEKDKAR PHPLGYG KNIGS+GDKGWIEYLL+NANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 IFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFRVNH 180
           IF   SH FLCAA EYVTAVKKLS +VVELIAEGLKME RNAISKLLNDEKADCCFRVNH
Sbjct: 121 IF---SHDFLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPEPQALGGRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAFFVN 240
           YPPCPE Q L GRNMIGFGEHTDPQIIS+LRSNNSTGLQICLRDGAWVSVPPD GA FVN
Sbjct: 181 YPPCPEMQGLSGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKV+ DPNRERVSMIYFGGPPLSEKI PLP+LLE+GEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLYKE 300

Query: 301 FTWWEYKTAAYKTKLADHRLGAFEKSPNS 330
           FTWWEYKTAAYK+KLAD+RLGAFEKSP S
Sbjct: 301 FTWWEYKTAAYKSKLADYRLGAFEKSPIS 326

BLAST of ClCG01G024630 vs. NCBI nr
Match: KAA0052541.1 (gibberellin 2-beta-dioxygenase-like [Cucumis melo var. makuwa] >TYK13284.1 gibberellin 2-beta-dioxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 612.8 bits (1579), Expect = 1.7e-171
Identity = 298/329 (90.58%), Postives = 309/329 (93.92%), Query Frame = 0

Query: 1   MVVLSHPTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNHR 60
           MVVLS PTLDLNQYSLLRSTCKPTASFA+IPEIDLSDPNAK +IVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANPLP 120
           VPIELMTKLEDESLCFFKLSESEKDKAR PHPLGYG KNIGS+GDKGWIEYLL+NANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 IFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFRVNH 180
           IF   SH FL AA EYVTAVKKLS +VVELIAEGLKME RNAISKLLNDEKADCCFRVNH
Sbjct: 121 IF---SHDFLRAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPEPQALGGRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAFFVN 240
           YPPCPE Q L GRNMIGFGEHTDPQIIS+LRSNNSTGLQICLRDGAWVSVPPD GA FVN
Sbjct: 181 YPPCPEMQGLSGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKV+ DPNRERVSMIYFGGPPLSEKI PLP+LLE+GEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLYKE 300

Query: 301 FTWWEYKTAAYKTKLADHRLGAFEKSPNS 330
           FTWWEYKTAAYK+KLAD+RLGAFEKSP S
Sbjct: 301 FTWWEYKTAAYKSKLADYRLGAFEKSPIS 326

BLAST of ClCG01G024630 vs. NCBI nr
Match: AUB30295.1 (gibberellin 2-beta-dioxygenase 8-like protein [Luffa aegyptiaca])

HSP 1 Score: 597.0 bits (1538), Expect = 9.6e-167
Identity = 286/331 (86.40%), Postives = 311/331 (93.96%), Query Frame = 0

Query: 1   MVVLSHPTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNHR 60
           MVVLS P L L+QYSLLR+TCKPTASF EIPEIDLSDPNA+S+IV+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANPLP 120
           VPIELMTKLE+E+LCFFKLS+SEKDKAR P PLGYGCKNIGS+GDKGWIEYLLL ANPLP
Sbjct: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120

Query: 121 IFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFRVNH 180
           IFSQNS +FL AA+EYVTAVKKLS +V ELIAEGLK+EPRNAISKLLNDEK+DCCFRVNH
Sbjct: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180

Query: 181 YPPCPEPQALG--GRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAFF 240
           YPPCP+ QALG  GRNMIGFGEHTDPQIIS+LRSNNSTGLQICLRDGAWVSVPPDA A F
Sbjct: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240

Query: 241 VNVGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESLY 300
           VNVGD+LQVM+NGRFKSVKHKV+AD NRERVSMIYFGGPPLSEKIAPLP++L +GEESLY
Sbjct: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTKLADHRLGAFEKSPNS 330
           KEFTWWEYKTAAYKT+LAD+RLG FEK+ NS
Sbjct: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 331

BLAST of ClCG01G024630 vs. NCBI nr
Match: NP_001295796.1 (gibberellin 2-beta-dioxygenase-like [Cucumis sativus] >CCG14218.1 GA 2-oxidase [Cucumis sativus])

HSP 1 Score: 593.2 bits (1528), Expect = 1.4e-165
Identity = 289/329 (87.84%), Postives = 305/329 (92.71%), Query Frame = 0

Query: 1   MVVLSH-PTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNH 60
           MVVLS  PTLD NQYSLLRSTCKPTASFA+IPEIDLSDPNAK +IVKACEEFGFFKLVNH
Sbjct: 1   MVVLSQPPTLDFNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNH 60

Query: 61  RVPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANPL 120
            VP+ELMTKLEDESLCFFKLS+SEKDKAR+PHPLGYG KNIGS+GDKGWIEYLLLNANPL
Sbjct: 61  GVPVELMTKLEDESLCFFKLSKSEKDKARTPHPLGYGSKNIGSNGDKGWIEYLLLNANPL 120

Query: 121 PIFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFRVN 180
           PIF   SH FLCAA EYVTAVKKLS +VVELIAEGLKME RNAISKLL DEKADCCFRVN
Sbjct: 121 PIF---SHDFLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLKDEKADCCFRVN 180

Query: 181 HYPPCPEPQALGG-RNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAFF 240
           HYPPCPE Q L G  NMIGFGEHTDPQI+S+LRSNNSTGLQICLRDGAWVSVP DA AFF
Sbjct: 181 HYPPCPEMQGLSGLNNMIGFGEHTDPQILSILRSNNSTGLQICLRDGAWVSVPADAAAFF 240

Query: 241 VNVGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESLY 300
           VNVGD LQVMTNGRFKSVKHKV+ DPNRERVSMIYFGGPPLSEKI PLP++L++GEESLY
Sbjct: 241 VNVGDVLQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPEVLKDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTKLADHRLGAFEKSP 328
           KEFTWWEYKTAAYK+KLAD+RLGAFEKSP
Sbjct: 301 KEFTWWEYKTAAYKSKLADYRLGAFEKSP 326

BLAST of ClCG01G024630 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 4.1e-120
Identity = 214/333 (64.26%), Postives = 255/333 (76.58%), Query Frame = 0

Query: 1   MVVLSHPTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNHR 60
           MVVLS P   LNQ+ LL+   K T  F  IP +DL+ P+AK+ IV AC +FGFFKLVNH 
Sbjct: 1   MVVLSQPA--LNQFFLLK-PFKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHG 60

Query: 61  VPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANP-- 120
           VP+ELM  LE+E+L FFK S+SEKD+A  P P GYG K IG +GD GW+EYLLLN NP  
Sbjct: 61  VPLELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDV 120

Query: 121 -----LPIFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKAD 180
                L IF +N H F    E Y+TAVK +   V+EL+AEGL +  RN +S+LL DEK+D
Sbjct: 121 ISPKSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSD 180

Query: 181 CCFRVNHYPPCPEPQALGGRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPD 240
            CFR+NHYPPCPE QAL  RN++GFGEHTDPQIISVLRSN+++GLQICL DG WVSVPPD
Sbjct: 181 SCFRLNHYPPCPEVQAL-NRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPD 240

Query: 241 AGAFFVNVGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEG 300
             +FF+NVGDALQVMTNGRFKSVKH+VLAD  + R+SMIYFGGP LSE IAPLP ++ +G
Sbjct: 241 QTSFFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKG 300

Query: 301 EESLYKEFTWWEYKTAAYKTKLADHRLGAFEKS 327
           EE LYKEFTW EYK AAY ++LAD+RL  F+KS
Sbjct: 301 EECLYKEFTWCEYKKAAYTSRLADNRLAPFQKS 329

BLAST of ClCG01G024630 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 7.2e-109
Identity = 198/336 (58.93%), Postives = 247/336 (73.51%), Query Frame = 0

Query: 1   MVVLSHPTLDLNQYSLLRSTCKPTASFA--EIPEIDLSDPNAKSNIVKACEEFGFFKLVN 60
           MVVL  P    N  SL+  T KP        IP ++L+DP AK+ IVKACEEFGFFK+VN
Sbjct: 1   MVVLPQPVTLDNHISLI-PTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANP 120
           H V  ELMT+LE E++ FF L +S K++A  P P GYG K IG +GD GWIEYLLLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPIFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEK 180
                    +F Q    F  + EEY+  +K++S +V+E++AE L +EPR+ +SK+L DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 ADCCFRVNHYPPCPEPQALGGRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVP 240
           +D C R+NHYP   E      +  +GFGEHTDPQIISVLRSNN+ GLQIC++DG+WV+VP
Sbjct: 181 SDSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 PDAGAFFVNVGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLE 300
           PD  +FF+NVGDALQVMTNGRFKSVKH+VLAD  R R+SMIYFGGPPLS+KIAPLP L+ 
Sbjct: 241 PDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVP 300

Query: 301 EGEESLYKEFTWWEYKTAAYKTKLADHRLGAFEKSP 328
           E ++ LYKEFTW +YK++AYK+KL D+RLG FEK P
Sbjct: 301 EQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEKQP 333

BLAST of ClCG01G024630 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 7.5e-106
Identity = 184/328 (56.10%), Postives = 255/328 (77.74%), Query Frame = 0

Query: 1   MVVLSHPTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNHR 60
           MV+LS PT +  QY+ +R+    T S + IP +DLS P+AK+ IVKACE+FGFFK++NH 
Sbjct: 1   MVLLSKPTSE--QYTYVRNNMPITFS-SSIPLVDLSKPDAKTLIVKACEDFGFFKVINHG 60

Query: 61  VPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANP-- 120
           +P++ +++LE E+  FF L ++EK+KA   +P GYG K IG +GD GWIEYLLL  N   
Sbjct: 61  IPLDAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTNQDH 120

Query: 121 -LPIFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFR 180
              ++ ++   F    ++Y  A++ ++ ++++L+AEGLK++P+N  SKL+ D+++DC FR
Sbjct: 121 NFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFR 180

Query: 181 VNHYPPCPEPQALGGRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAF 240
           VNHYP CPE  A+ G N+IGFGEHTDPQIIS+LRSNN++G QI LRDG+W+SVPPD  +F
Sbjct: 181 VNHYPACPE-LAINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSSF 240

Query: 241 FVNVGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESL 300
           F+NVGD+LQVMTNGRFKSV+H+VLA+    R+SMIYF GPPLSEKIAPLP L+ +G+ESL
Sbjct: 241 FINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPSLM-KGKESL 300

Query: 301 YKEFTWWEYKTAAYKTKLADHRLGAFEK 326
           YKEFTW+EYK++ Y ++LAD+RLG +E+
Sbjct: 301 YKEFTWFEYKSSTYGSRLADNRLGNYER 323

BLAST of ClCG01G024630 vs. ExPASy Swiss-Prot
Match: O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 2.6e-98
Identity = 179/334 (53.59%), Postives = 233/334 (69.76%), Query Frame = 0

Query: 1   MVVLSHPTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNHR 60
           ++VL   + D N Y  +   CKP      IP IDL+D +AK+ IVKACEEFGFFK++NH 
Sbjct: 2   VIVLQPASFDSNLY--VNPKCKPRPVL--IPVIDLTDSDAKTQIVKACEEFGFFKVINHG 61

Query: 61  VPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNAN--- 120
           V  +L+T+LE E++ FF L  S KDKA  P P GYG K IG +GD GW+EY+LLNAN   
Sbjct: 62  VRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCL 121

Query: 121 ----PLPIFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKAD 180
                  IF      F  A EEY+  +K++SS+ +E++ E LK+EP+  +S+L+  +++D
Sbjct: 122 ESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESD 181

Query: 181 CCFRVNHYPPCPEPQALGGRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPD 240
            C R+NHY   PE +    +  IGFGEHTDPQ+IS+LRSN++ GLQIC++DG WV V PD
Sbjct: 182 SCLRMNHY---PEKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPD 241

Query: 241 AGAFFVNVGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEG 300
             +FFV VGD LQVMTNGRFKSVKH+V+ +  R R+SMIYF GPPLSEKIAPL  L+ + 
Sbjct: 242 HSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQ 301

Query: 301 EESLYKEFTWWEYKTAAYKTKLADHRLGAFEKSP 328
           ++ LY EFTW +YK +AYKTKL D+RLG FEK P
Sbjct: 302 DDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEKRP 328

BLAST of ClCG01G024630 vs. ExPASy Swiss-Prot
Match: Q8LEA2 (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 358.2 bits (918), Expect = 9.8e-98
Identity = 173/312 (55.45%), Postives = 233/312 (74.68%), Query Frame = 0

Query: 23  PTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNHRVPIELMTKLEDESLCFFKLSES 82
           P + F+ IP ID+SDP +K  +VKACE+FGFFK++NH V  EL++ LE E++ FF L +S
Sbjct: 11  PKSGFSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKS 70

Query: 83  EKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNAN------PL-PIFSQNSHTFLCAAEE 142
           EK +  + +P GYG   IG +GD GW+EYLL+NAN      PL P   ++  TF  A EE
Sbjct: 71  EKTQV-AGYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEE 130

Query: 143 YVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFRVNHYPPCP--EPQALGGR 202
           Y T+V+K++  V+E I +GL ++PRN +SKL++D+  D   R+NHYPPCP    +  GG+
Sbjct: 131 YTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGK 190

Query: 203 NMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAFFVNVGDALQVMTNGRF 262
           N+IGFGEHTDPQIISVLRSNN++GLQI L DG+W+SVPPD  +FF NVGD+LQVMTNGRF
Sbjct: 191 NVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRF 250

Query: 263 KSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESLYKEFTWWEYKTAAYKT 322
           KSV+H+VLA+  + RVSMIYF GP L+++IAPL  L++  +E LY+EFTW EYK + Y +
Sbjct: 251 KSVRHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNS 310

Query: 323 KLADHRLGAFEK 326
           +L+D+RL  FE+
Sbjct: 311 RLSDNRLQQFER 321

BLAST of ClCG01G024630 vs. ExPASy TrEMBL
Match: A0A1S3AZ81 (gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103484366 PE=3 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 3.3e-173
Identity = 299/329 (90.88%), Postives = 310/329 (94.22%), Query Frame = 0

Query: 1   MVVLSHPTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNHR 60
           MVVLS PTLDLNQYSLLRSTCKPTASFA+IPEIDLSDPNAK +IVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANPLP 120
           VPIELMTKLEDESLCFFKLSESEKDKAR PHPLGYG KNIGS+GDKGWIEYLL+NANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 IFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFRVNH 180
           IF   SH FLCAA EYVTAVKKLS +VVELIAEGLKME RNAISKLLNDEKADCCFRVNH
Sbjct: 121 IF---SHDFLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPEPQALGGRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAFFVN 240
           YPPCPE Q L GRNMIGFGEHTDPQIIS+LRSNNSTGLQICLRDGAWVSVPPD GA FVN
Sbjct: 181 YPPCPEMQGLSGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKV+ DPNRERVSMIYFGGPPLSEKI PLP+LLE+GEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLYKE 300

Query: 301 FTWWEYKTAAYKTKLADHRLGAFEKSPNS 330
           FTWWEYKTAAYK+KLAD+RLGAFEKSP S
Sbjct: 301 FTWWEYKTAAYKSKLADYRLGAFEKSPIS 326

BLAST of ClCG01G024630 vs. ExPASy TrEMBL
Match: A0A5A7UGB1 (Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009030 PE=3 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 8.1e-172
Identity = 298/329 (90.58%), Postives = 309/329 (93.92%), Query Frame = 0

Query: 1   MVVLSHPTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNHR 60
           MVVLS PTLDLNQYSLLRSTCKPTASFA+IPEIDLSDPNAK +IVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANPLP 120
           VPIELMTKLEDESLCFFKLSESEKDKAR PHPLGYG KNIGS+GDKGWIEYLL+NANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 IFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFRVNH 180
           IF   SH FL AA EYVTAVKKLS +VVELIAEGLKME RNAISKLLNDEKADCCFRVNH
Sbjct: 121 IF---SHDFLRAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPEPQALGGRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAFFVN 240
           YPPCPE Q L GRNMIGFGEHTDPQIIS+LRSNNSTGLQICLRDGAWVSVPPD GA FVN
Sbjct: 181 YPPCPEMQGLSGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKV+ DPNRERVSMIYFGGPPLSEKI PLP+LLE+GEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLYKE 300

Query: 301 FTWWEYKTAAYKTKLADHRLGAFEKSPNS 330
           FTWWEYKTAAYK+KLAD+RLGAFEKSP S
Sbjct: 301 FTWWEYKTAAYKSKLADYRLGAFEKSPIS 326

BLAST of ClCG01G024630 vs. ExPASy TrEMBL
Match: A0A2H4V3T7 (Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 4.6e-167
Identity = 286/331 (86.40%), Postives = 311/331 (93.96%), Query Frame = 0

Query: 1   MVVLSHPTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNHR 60
           MVVLS P L L+QYSLLR+TCKPTASF EIPEIDLSDPNA+S+IV+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANPLP 120
           VPIELMTKLE+E+LCFFKLS+SEKDKAR P PLGYGCKNIGS+GDKGWIEYLLL ANPLP
Sbjct: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120

Query: 121 IFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFRVNH 180
           IFSQNS +FL AA+EYVTAVKKLS +V ELIAEGLK+EPRNAISKLLNDEK+DCCFRVNH
Sbjct: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180

Query: 181 YPPCPEPQALG--GRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAFF 240
           YPPCP+ QALG  GRNMIGFGEHTDPQIIS+LRSNNSTGLQICLRDGAWVSVPPDA A F
Sbjct: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240

Query: 241 VNVGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESLY 300
           VNVGD+LQVM+NGRFKSVKHKV+AD NRERVSMIYFGGPPLSEKIAPLP++L +GEESLY
Sbjct: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTKLADHRLGAFEKSPNS 330
           KEFTWWEYKTAAYKT+LAD+RLG FEK+ NS
Sbjct: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 331

BLAST of ClCG01G024630 vs. ExPASy TrEMBL
Match: S0BE28 (GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox4 PE=2 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 6.7e-166
Identity = 289/329 (87.84%), Postives = 305/329 (92.71%), Query Frame = 0

Query: 1   MVVLSH-PTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNH 60
           MVVLS  PTLD NQYSLLRSTCKPTASFA+IPEIDLSDPNAK +IVKACEEFGFFKLVNH
Sbjct: 1   MVVLSQPPTLDFNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNH 60

Query: 61  RVPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANPL 120
            VP+ELMTKLEDESLCFFKLS+SEKDKAR+PHPLGYG KNIGS+GDKGWIEYLLLNANPL
Sbjct: 61  GVPVELMTKLEDESLCFFKLSKSEKDKARTPHPLGYGSKNIGSNGDKGWIEYLLLNANPL 120

Query: 121 PIFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFRVN 180
           PIF   SH FLCAA EYVTAVKKLS +VVELIAEGLKME RNAISKLL DEKADCCFRVN
Sbjct: 121 PIF---SHDFLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLKDEKADCCFRVN 180

Query: 181 HYPPCPEPQALGG-RNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAFF 240
           HYPPCPE Q L G  NMIGFGEHTDPQI+S+LRSNNSTGLQICLRDGAWVSVP DA AFF
Sbjct: 181 HYPPCPEMQGLSGLNNMIGFGEHTDPQILSILRSNNSTGLQICLRDGAWVSVPADAAAFF 240

Query: 241 VNVGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESLY 300
           VNVGD LQVMTNGRFKSVKHKV+ DPNRERVSMIYFGGPPLSEKI PLP++L++GEESLY
Sbjct: 241 VNVGDVLQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPEVLKDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTKLADHRLGAFEKSP 328
           KEFTWWEYKTAAYK+KLAD+RLGAFEKSP
Sbjct: 301 KEFTWWEYKTAAYKSKLADYRLGAFEKSP 326

BLAST of ClCG01G024630 vs. ExPASy TrEMBL
Match: A0A0A0KIK7 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G523390 PE=3 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.0e-161
Identity = 285/329 (86.63%), Postives = 301/329 (91.49%), Query Frame = 0

Query: 1   MVVLSH-PTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNH 60
           MVVLS  PTLD NQYSLLRSTCKPTASFA+IPEIDLSDPNAK +IVKACEEFGFFKLVNH
Sbjct: 1   MVVLSQPPTLDFNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNH 60

Query: 61  RVPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANPL 120
            VP+ELMTKLEDESLCFFKLS+SEKDKAR+PHPLGY     GS+GDKGWIEYLLLNANPL
Sbjct: 61  GVPVELMTKLEDESLCFFKLSKSEKDKARTPHPLGY-----GSNGDKGWIEYLLLNANPL 120

Query: 121 PIFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFRVN 180
           PIF   SH FLCAA EYVTAVKKLS +VVELIAEGLKME RNAISKLL DEKADCCFRVN
Sbjct: 121 PIF---SHDFLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLKDEKADCCFRVN 180

Query: 181 HYPPCPEPQALGG-RNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAFF 240
           HYPPCPE Q L G  NMIGFGEHTDPQI+S+LRSNNSTGLQICLRDGAWVSVP DA AFF
Sbjct: 181 HYPPCPEMQGLSGLNNMIGFGEHTDPQILSILRSNNSTGLQICLRDGAWVSVPADAAAFF 240

Query: 241 VNVGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESLY 300
           VNVGD LQVMTNGRFKSVKHKV+ DPNRERVSMIYFGGPPLSEKI PLP++L++GEESLY
Sbjct: 241 VNVGDVLQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPEVLKDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTKLADHRLGAFEKSP 328
           KEFTWWEYKTAAYK+KLAD+RLGAFEKSP
Sbjct: 301 KEFTWWEYKTAAYKSKLADYRLGAFEKSP 321

BLAST of ClCG01G024630 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 395.2 bits (1014), Expect = 5.1e-110
Identity = 198/336 (58.93%), Postives = 247/336 (73.51%), Query Frame = 0

Query: 1   MVVLSHPTLDLNQYSLLRSTCKPTASFA--EIPEIDLSDPNAKSNIVKACEEFGFFKLVN 60
           MVVL  P    N  SL+  T KP        IP ++L+DP AK+ IVKACEEFGFFK+VN
Sbjct: 1   MVVLPQPVTLDNHISLI-PTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANP 120
           H V  ELMT+LE E++ FF L +S K++A  P P GYG K IG +GD GWIEYLLLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPIFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEK 180
                    +F Q    F  + EEY+  +K++S +V+E++AE L +EPR+ +SK+L DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 ADCCFRVNHYPPCPEPQALGGRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVP 240
           +D C R+NHYP   E      +  +GFGEHTDPQIISVLRSNN+ GLQIC++DG+WV+VP
Sbjct: 181 SDSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 PDAGAFFVNVGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLE 300
           PD  +FF+NVGDALQVMTNGRFKSVKH+VLAD  R R+SMIYFGGPPLS+KIAPLP L+ 
Sbjct: 241 PDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVP 300

Query: 301 EGEESLYKEFTWWEYKTAAYKTKLADHRLGAFEKSP 328
           E ++ LYKEFTW +YK++AYK+KL D+RLG FEK P
Sbjct: 301 EQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEKQP 333

BLAST of ClCG01G024630 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 360.1 bits (923), Expect = 1.8e-99
Identity = 179/334 (53.59%), Postives = 233/334 (69.76%), Query Frame = 0

Query: 1   MVVLSHPTLDLNQYSLLRSTCKPTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNHR 60
           ++VL   + D N Y  +   CKP      IP IDL+D +AK+ IVKACEEFGFFK++NH 
Sbjct: 2   VIVLQPASFDSNLY--VNPKCKPRPVL--IPVIDLTDSDAKTQIVKACEEFGFFKVINHG 61

Query: 61  VPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNAN--- 120
           V  +L+T+LE E++ FF L  S KDKA  P P GYG K IG +GD GW+EY+LLNAN   
Sbjct: 62  VRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCL 121

Query: 121 ----PLPIFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKAD 180
                  IF      F  A EEY+  +K++SS+ +E++ E LK+EP+  +S+L+  +++D
Sbjct: 122 ESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESD 181

Query: 181 CCFRVNHYPPCPEPQALGGRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPD 240
            C R+NHY   PE +    +  IGFGEHTDPQ+IS+LRSN++ GLQIC++DG WV V PD
Sbjct: 182 SCLRMNHY---PEKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPD 241

Query: 241 AGAFFVNVGDALQVMTNGRFKSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEG 300
             +FFV VGD LQVMTNGRFKSVKH+V+ +  R R+SMIYF GPPLSEKIAPL  L+ + 
Sbjct: 242 HSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQ 301

Query: 301 EESLYKEFTWWEYKTAAYKTKLADHRLGAFEKSP 328
           ++ LY EFTW +YK +AYKTKL D+RLG FEK P
Sbjct: 302 DDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEKRP 328

BLAST of ClCG01G024630 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 358.2 bits (918), Expect = 7.0e-99
Identity = 173/312 (55.45%), Postives = 233/312 (74.68%), Query Frame = 0

Query: 23  PTASFAEIPEIDLSDPNAKSNIVKACEEFGFFKLVNHRVPIELMTKLEDESLCFFKLSES 82
           P + F+ IP ID+SDP +K  +VKACE+FGFFK++NH V  EL++ LE E++ FF L +S
Sbjct: 11  PKSGFSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKS 70

Query: 83  EKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNAN------PL-PIFSQNSHTFLCAAEE 142
           EK +  + +P GYG   IG +GD GW+EYLL+NAN      PL P   ++  TF  A EE
Sbjct: 71  EKTQV-AGYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEE 130

Query: 143 YVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFRVNHYPPCP--EPQALGGR 202
           Y T+V+K++  V+E I +GL ++PRN +SKL++D+  D   R+NHYPPCP    +  GG+
Sbjct: 131 YTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGK 190

Query: 203 NMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAFFVNVGDALQVMTNGRF 262
           N+IGFGEHTDPQIISVLRSNN++GLQI L DG+W+SVPPD  +FF NVGD+LQVMTNGRF
Sbjct: 191 NVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRF 250

Query: 263 KSVKHKVLADPNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESLYKEFTWWEYKTAAYKT 322
           KSV+H+VLA+  + RVSMIYF GP L+++IAPL  L++  +E LY+EFTW EYK + Y +
Sbjct: 251 KSVRHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNS 310

Query: 323 KLADHRLGAFEK 326
           +L+D+RL  FE+
Sbjct: 311 RLSDNRLQQFER 321

BLAST of ClCG01G024630 vs. TAIR 10
Match: AT1G30040.2 (gibberellin 2-oxidase )

HSP 1 Score: 272.3 bits (695), Expect = 5.0e-73
Identity = 140/256 (54.69%), Postives = 178/256 (69.53%), Query Frame = 0

Query: 1   MVVLSHPTLDLNQYSLLRSTCKPTASFA--EIPEIDLSDPNAKSNIVKACEEFGFFKLVN 60
           MVVL  P    N  SL+  T KP        IP ++L+DP AK+ IVKACEEFGFFK+VN
Sbjct: 1   MVVLPQPVTLDNHISLI-PTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPIELMTKLEDESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANP 120
           H V  ELMT+LE E++ FF L +S K++A  P P GYG K IG +GD GWIEYLLLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPIFSQNSHTFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEK 180
                    +F Q    F  + EEY+  +K++S +V+E++AE L +EPR+ +SK+L DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 ADCCFRVNHYPPCPEPQALGGRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVP 240
           +D C R+NHYP   E      +  +GFGEHTDPQIISVLRSNN+ GLQIC++DG+WV+VP
Sbjct: 181 SDSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 PDAGAFFVNVGDALQV 248
           PD  +FF+NVGDALQV
Sbjct: 241 PDHSSFFINVGDALQV 253

BLAST of ClCG01G024630 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 271.9 bits (694), Expect = 6.6e-73
Identity = 144/321 (44.86%), Postives = 196/321 (61.06%), Query Frame = 0

Query: 17  LRSTCKPTASFAE--IPEIDLS---DPNAKSNIVKACEEFGFFKLVNHRVPIELMTKLED 76
           L++T K T S  E   P ID S          IVKACE  GFFK++NH V  E++ + E 
Sbjct: 9   LQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEH 68

Query: 77  ESLCFFKLSESEKDKARSPHPLGYGCKNIGSSGDKGWIEYLLLNANPLPIFSQN---SH- 136
           E   FF   ES+K +A    P GYGCKNIG +GD G +EYLLL+ANP  +  ++   SH 
Sbjct: 69  EGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHD 128

Query: 137 ---TFLCAAEEYVTAVKKLSSQVVELIAEGLKMEPRNAISKLLNDEKADCCFRVNHYPPC 196
               F  A  +Y+  V+ L+ ++++L  E L  +  + +S+L+ D ++D   R+NHYPP 
Sbjct: 129 DPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPA 188

Query: 197 PEPQALGGRNMIGFGEHTDPQIISVLRSNNSTGLQICLRDGAWVSVPPDAGAFFVNVGDA 256
             P AL G   IGFGEH+DPQI++VLRSN+  GL+IC RDG W+ +P D   FFV VGD 
Sbjct: 189 --PYALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGDC 248

Query: 257 LQVMTNGRFKSVKHKVLAD-PNRERVSMIYFGGPPLSEKIAPLPQLLEEGEESLYKEFTW 316
           LQ +TNGRF SV+H+VLA+   + R+S +YF  PPL  KI+PLP+++       Y  FTW
Sbjct: 249 LQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSFTW 308

Query: 317 WEYKTAAYKTKLADHRLGAFE 325
            +YK A Y  +L   RL  F+
Sbjct: 309 GDYKKATYSLRLDVPRLEFFK 327

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038883200.14.4e-18093.92gibberellin 2-beta-dioxygenase-like [Benincasa hispida][more]
XP_008439633.16.8e-17390.88PREDICTED: gibberellin 2-beta-dioxygenase-like [Cucumis melo][more]
KAA0052541.11.7e-17190.58gibberellin 2-beta-dioxygenase-like [Cucumis melo var. makuwa] >TYK13284.1 gibbe... [more]
AUB30295.19.6e-16786.40gibberellin 2-beta-dioxygenase 8-like protein [Luffa aegyptiaca][more]
NP_001295796.11.4e-16587.84gibberellin 2-beta-dioxygenase-like [Cucumis sativus] >CCG14218.1 GA 2-oxidase [... [more]
Match NameE-valueIdentityDescription
Q9XG834.1e-12064.26Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=... [more]
Q9XFR97.2e-10958.93Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ... [more]
Q9SQ807.5e-10656.10Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1[more]
O646922.6e-9853.59Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ... [more]
Q8LEA29.8e-9855.45Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A1S3AZ813.3e-17390.88gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103484366 PE=3... [more]
A0A5A7UGB18.1e-17290.58Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A2H4V3T74.6e-16786.40Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 S... [more]
S0BE286.7e-16687.84GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox4 PE=2 SV=1[more]
A0A0A0KIK71.0e-16186.63Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G... [more]
Match NameE-valueIdentityDescription
AT1G30040.15.1e-11058.93gibberellin 2-oxidase [more]
AT2G34555.11.8e-9953.59gibberellin 2-oxidase 3 [more]
AT1G78440.17.0e-9955.45Arabidopsis thaliana gibberellin 2-oxidase 1 [more]
AT1G30040.25.0e-7354.69gibberellin 2-oxidase [more]
AT1G02400.16.6e-7344.86gibberellin 2-oxidase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 9..318
e-value: 2.2E-80
score: 272.4
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 173..275
e-value: 1.1E-27
score: 96.5
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 30..116
e-value: 2.7E-13
score: 50.7
NoneNo IPR availablePANTHERPTHR47990:SF121GIBBERELLIN 2-BETA-DIOXYGENASE 2coord: 10..326
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 10..326
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 21..317
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 171..277
score: 12.205518

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG01G024630.1ClCG01G024630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding