ClCG01G018980 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG01G018980
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	L-ascorbate oxidase
Location	CG_Chr01: 33422766 .. 33425097 (+)
RNA-Seq Expression	ClCG01G018980
Synteny	ClCG01G018980

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCCGTTTACTTGGACCAGGAGTTGATTGGGTCAACCTCATGTAGGATATTTATATAAACCCCAAGTTGTTGTGCAAAAAATCAATCTCAATGATTTTTATTTTTTAGGTTTTGAGTTTCAGGAGAATCACCAAAACGTTTCAACAAATGGCTTCGTTGTTACAAATTAGACAATACTTGTTAATTCTTCTCTGCTTCTTCTTGTTCTTATTACAATCTCCAACTGCAGAATCAAGAACTCTGACCTATAAATGGGAAGTGAAGTCCGAATTGAAATCCCCCGACTGCTTTAAAAAACTTACCATGACCATTAATGGCCGATCTCCAGGTCCCACAATCTACGCCCACCAAGGTGACACCGTCATCGTTGAGCTCAAGAACAGCCTCGGTACCGAAAACGTCGCAATCCACTGGCATGGAATCCGACAGGTACCCCAGATTTCATCTCACTCCAATTACTTAGATGATTTTGTATCATTTTTTTTCCTCAAGTGTTGTATTTTGTGTAATTTTATTGAAGATTGGAACACCTTGGAGTGATGGAACAGAAGGTGTCACTCAATGTCCTATTTTACCTGGTGAAACCTTCAGATATCAGTTCGTTGTAGACAGAGTAAGAGCTGGACCCACTGGTTTTGTCTTGTTCATCTCAAAAGCTCCATCATATATTTTGGGTTTGACCGTTGTGAATCTTTTGAAATGTGCAGCCAGGGACTTATCTATACCATGCCCATTACGGTATGCAAAGAGAAGATGGTCTCTATGGATCCATTGTGGTTTGGCTTCCAGAAGGACAAACAGAGCCTTTTTCTTACGATTACGATCGTAATCTCATCCTCACAGATTGGTATCATAAAAGCAGCCAAGAACACGCCGCTGGCTTGGCTACCCCTGGCTCTGGCTTCACCTGGGTGGGAGAGCCTGATTCCCTTTTGATACAAGGAAGAGGGAGATTCAATTGCTCTGTCTTGGGATCTTCCAGTTTAGCCTCTGCTACCTGTAATTCATCCAACCCAGAATGTGTTCTTTATCCATTGACAGTCGTACCAGGAAAAACTTACCGATTGAGGGTCTCTAGTCTTACTTCTCTTTCAGCTCTCAGTTTTCAAATTGAGGTAATTACAAAATCATCTACTTAATTTTCATTTCCCTCCTTGAAATTCATCTGAAATGACTTAAACTCTCTGTTTTGGAAACAGGGGCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTTCAAGTAAATAATTTGTTCATATACTCGGGAGAAACATATTCCGTGTTGATAAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTGAGCCGACAAAGAACCACCCCCCCTGGTTTAGCCATTTTCAATTACTACCCAAATCATCAGAGAAAACTCCCTCCAACAACGCCTCCGGCAGCACCTGCTTGGAACGATGTTGCACCTCGAATGGCTCAAAGCCACGCCATCAAAGCCCGTCAAGGTTTCGTCCACGCGCCGCCTAAAGCCGCCGACAAAGTCATCGTGCTTCTCAACACCCAAAACACCGTGAATGGACGCCGCCGGTGGTCGTTGAATAACGTTTCCTTCAACATGCCACACACCCCTTACCTTATCGCCCTGAAGCACAATCTTCTCCACACCTTCAGCCAGGAAAAACCACCCACAGGATACGATTTCAAGAACTACGACATTTTCAATCCGGCGCCCAACCCTGAAGCGACGGTGAGCGATGCCATATTCCGGCTGGACTTCAATTCGACAGTGGACATCATTTTGCAAAACGCCAATATGATGAGCCCAAACAACAGCGAAACGCATCCTTGGCATTTGCACGGCCACGATTTCTGGGTATTGGGATACGGGGAAGGGAAATTCGATATCTATAAAGATCCAAGCAAGTACAATTTGGAGAATCCGATCATGAAGAACACAGTGCCGTTGCACCCATATGGATGGACGGCTTTGAGATTTGTGGCGGATAACCCTGGGGTTTGGGCTTTCCACTGCCACATCGACGCCCATTTCTTTATGGGAATGGGTGTTGTTTTTGCAGAAGGGATTGACAAGGTTCAGAAATTGCCTACTTCCATTATGGGATGCGGCGAGAGCAGGAGGTTCTTAAGGCCTTAACTCATTTCCATCCTCATCATTCTTTATGTTGAACCATATTCTTTTAAATAATAGAGTCTCTGTGAATGAATAGGTAAATGTTGTTCTATTCTTTGTTATAGTAATTGCAATCCTTGTTCCCTACAGGAAAATTTGTCATAACTATTGAAATAATTGAAAGAAGAATACGTTG

mRNA sequence

ATGGAGCCGAGAATCACCAAAACGTTTCAACAAATGGCTTCGTTGTTACAAATTAGACAATACTTGTTAATTCTTCTCTGCTTCTTCTTGTTCTTATTACAATCTCCAACTGCAGAATCAAGAACTCTGACCTATAAATGGGAAGTGAAGTCCGAATTGAAATCCCCCGACTGCTTTAAAAAACTTACCATGACCATTAATGGCCGATCTCCAGGTCCCACAATCTACGCCCACCAAGGTGACACCGTCATCGTTGAGCTCAAGAACAGCCTCGGTACCGAAAACGTCGCAATCCACTGGCATGGAATCCGACAGGTACCCCAGATTTCATCTCACTCCAATTACTTAGATGATTTTATTGGAACACCTTGGAGTGATGGAACAGAAGGTGTCACTCAATGTCCTATTTTACCTGGTGAAACCTTCAGATATCAGTTCGTTGTAGACAGACCAGGGACTTATCTATACCATGCCCATTACGGTATGCAAAGAGAAGATGGTCTCTATGGATCCATTGTGGTTTGGCTTCCAGAAGGACAAACAGAGCCTTTTTCTTACGATTACGATCGTAATCTCATCCTCACAGATTGGTATCATAAAAGCAGCCAAGAACACGCCGCTGGCTTGGCTACCCCTGGCTCTGGCTTCACCTGGGTGGGAGAGCCTGATTCCCTTTTGATACAAGGAAGAGGGAGATTCAATTGCTCTGTCTTGGGATCTTCCAGTTTAGCCTCTGCTACCTGTAATTCATCCAACCCAGAATGTGTTCTTTATCCATTGACAGTCGTACCAGGAAAAACTTACCGATTGAGGGTCTCTAGTCTTACTTCTCTTTCAGCTCTCAGTTTTCAAATTGAGGGGCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTTCAAGTAAATAATTTGTTCATATACTCGGGAGAAACATATTCCGTGTTGATAAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTGAGCCGACAAAGAACCACCCCCCCTGGTTTAGCCATTTTCAATTACTACCCAAATCATCAGAGAAAACTCCCTCCAACAACGCCTCCGGCAGCACCTGCTTGGAACGATGTTGCACCTCGAATGGCTCAAAGCCACGCCATCAAAGCCCGTCAAGGTTTCGTCCACGCGCCGCCTAAAGCCGCCGACAAAGTCATCGTGCTTCTCAACACCCAAAACACCGTGAATGGACGCCGCCGGTGGTCGTTGAATAACGTTTCCTTCAACATGCCACACACCCCTTACCTTATCGCCCTGAAGCACAATCTTCTCCACACCTTCAGCCAGGAAAAACCACCCACAGGATACGATTTCAAGAACTACGACATTTTCAATCCGGCGCCCAACCCTGAAGCGACGGTGAGCGATGCCATATTCCGGCTGGACTTCAATTCGACAGTGGACATCATTTTGCAAAACGCCAATATGATGAGCCCAAACAACAGCGAAACGCATCCTTGGCATTTGCACGGCCACGATTTCTGGGTATTGGGATACGGGGAAGGGAAATTCGATATCTATAAAGATCCAAGCAAGTACAATTTGGAGAATCCGATCATGAAGAACACAGTGCCGTTGCACCCATATGGATGGACGGCTTTGAGATTTGTGGCGGATAACCCTGGGGTTTGGGCTTTCCACTGCCACATCGACGCCCATTTCTTTATGGGAATGGGTGTTGTTTTTGCAGAAGGGATTGACAAGGTTCAGAAATTGCCTACTTCCATTATGGGATGCGGCGAGAGCAGGAGGTTCTTAAGGCCTTAACTCATTTCCATCCTCATCATTCTTTATGTTGAACCATATTCTTTTAAATAATAGAGTCTCTGTGAATGAATAGGTAAATGTTGTTCTATTCTTTGTTATAGTAATTGCAATCCTTGTTCCCTACAGGAAAATTTGTCATAACTATTGAAATAATTGAAAGAAGAATACGTTG

Coding sequence (CDS)

ATGGAGCCGAGAATCACCAAAACGTTTCAACAAATGGCTTCGTTGTTACAAATTAGACAATACTTGTTAATTCTTCTCTGCTTCTTCTTGTTCTTATTACAATCTCCAACTGCAGAATCAAGAACTCTGACCTATAAATGGGAAGTGAAGTCCGAATTGAAATCCCCCGACTGCTTTAAAAAACTTACCATGACCATTAATGGCCGATCTCCAGGTCCCACAATCTACGCCCACCAAGGTGACACCGTCATCGTTGAGCTCAAGAACAGCCTCGGTACCGAAAACGTCGCAATCCACTGGCATGGAATCCGACAGGTACCCCAGATTTCATCTCACTCCAATTACTTAGATGATTTTATTGGAACACCTTGGAGTGATGGAACAGAAGGTGTCACTCAATGTCCTATTTTACCTGGTGAAACCTTCAGATATCAGTTCGTTGTAGACAGACCAGGGACTTATCTATACCATGCCCATTACGGTATGCAAAGAGAAGATGGTCTCTATGGATCCATTGTGGTTTGGCTTCCAGAAGGACAAACAGAGCCTTTTTCTTACGATTACGATCGTAATCTCATCCTCACAGATTGGTATCATAAAAGCAGCCAAGAACACGCCGCTGGCTTGGCTACCCCTGGCTCTGGCTTCACCTGGGTGGGAGAGCCTGATTCCCTTTTGATACAAGGAAGAGGGAGATTCAATTGCTCTGTCTTGGGATCTTCCAGTTTAGCCTCTGCTACCTGTAATTCATCCAACCCAGAATGTGTTCTTTATCCATTGACAGTCGTACCAGGAAAAACTTACCGATTGAGGGTCTCTAGTCTTACTTCTCTTTCAGCTCTCAGTTTTCAAATTGAGGGGCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTTCAAGTAAATAATTTGTTCATATACTCGGGAGAAACATATTCCGTGTTGATAAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTGAGCCGACAAAGAACCACCCCCCCTGGTTTAGCCATTTTCAATTACTACCCAAATCATCAGAGAAAACTCCCTCCAACAACGCCTCCGGCAGCACCTGCTTGGAACGATGTTGCACCTCGAATGGCTCAAAGCCACGCCATCAAAGCCCGTCAAGGTTTCGTCCACGCGCCGCCTAAAGCCGCCGACAAAGTCATCGTGCTTCTCAACACCCAAAACACCGTGAATGGACGCCGCCGGTGGTCGTTGAATAACGTTTCCTTCAACATGCCACACACCCCTTACCTTATCGCCCTGAAGCACAATCTTCTCCACACCTTCAGCCAGGAAAAACCACCCACAGGATACGATTTCAAGAACTACGACATTTTCAATCCGGCGCCCAACCCTGAAGCGACGGTGAGCGATGCCATATTCCGGCTGGACTTCAATTCGACAGTGGACATCATTTTGCAAAACGCCAATATGATGAGCCCAAACAACAGCGAAACGCATCCTTGGCATTTGCACGGCCACGATTTCTGGGTATTGGGATACGGGGAAGGGAAATTCGATATCTATAAAGATCCAAGCAAGTACAATTTGGAGAATCCGATCATGAAGAACACAGTGCCGTTGCACCCATATGGATGGACGGCTTTGAGATTTGTGGCGGATAACCCTGGGGTTTGGGCTTTCCACTGCCACATCGACGCCCATTTCTTTATGGGAATGGGTGTTGTTTTTGCAGAAGGGATTGACAAGGTTCAGAAATTGCCTACTTCCATTATGGGATGCGGCGAGAGCAGGAGGTTCTTAAGGCCTTAA

Protein sequence

MEPRITKTFQQMASLLQIRQYLLILLCFFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRNLILTDWYHKSSQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSSNPECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAWNDVAPRMAQSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGESRRFLRP

Homology

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BLAST of ClCG01G018980 vs. NCBI nr
Match: XP_038881120.1 (L-ascorbate oxidase [Benincasa hispida])

HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 561/601 (93.34%), Postives = 573/601 (95.34%), Query Frame = 0


Query: 4   RITKTFQQMASLLQIRQYLLILLCFFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLT 63
           R TKT QQMASLLQ RQYLLILLCF LFL+QSP AESRTL YKWEVKSELKSPDCFKKLT
Sbjct: 10  RSTKTIQQMASLLQSRQYLLILLCFLLFLIQSPIAESRTLNYKWEVKSELKSPDCFKKLT 69

Query: 64  MTINGRSPGPTIYAHQGDTVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTP 123
           MTINGRSPGPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQ              IGTP
Sbjct: 70  MTINGRSPGPTIYAHQGDTVIVELKNSFSTENVAIHWHGIRQ--------------IGTP 129

Query: 124 WSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEP 183
           WSDGTEGVTQCPILPGETF+YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEP
Sbjct: 130 WSDGTEGVTQCPILPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEP 189

Query: 184 FSYDYDRNLILTDWYHKSSQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSL 243
           FSYDYDRNLILTDWYHK+S EHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS+ 
Sbjct: 190 FSYDYDRNLILTDWYHKTSHEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSST- 249

Query: 244 ASATCNSSNPECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPF 303
            SATCNSSNPEC+LYP+TVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPF
Sbjct: 250 -SATCNSSNPECILYPITVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPF 309

Query: 304 QVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPT 363
           QVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSR RTTPPGLAIFNYYPNHQRKLPPT
Sbjct: 310 QVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQRKLPPT 369

Query: 364 TPPAAPAWNDVAPRMAQSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVS 423
           TPPA PAWNDVAPR+AQSHAIKARQGF+HAPPKAADKVIVLLNTQNTVNGRRRWSLNNVS
Sbjct: 370 TPPAPPAWNDVAPRLAQSHAIKARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVS 429

Query: 424 FNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTV 483
           FNMPHTPYLIALKHNLLH FSQ+KPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTV
Sbjct: 430 FNMPHTPYLIALKHNLLHAFSQDKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTV 489

Query: 484 DIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLH 543
           DIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSK+NLENPIMKNTVPLH
Sbjct: 490 DIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKFNLENPIMKNTVPLH 549

Query: 544 PYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGESRRFLR 603
           PYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAEGID+VQKLPTSIMGCGES+RFLR
Sbjct: 550 PYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLR 594

Query: 604 P 605
           P
Sbjct: 610 P 594

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BLAST of ClCG01G018980 vs. NCBI nr
Match: XP_008440636.1 (PREDICTED: L-ascorbate oxidase [Cucumis melo] >KAA0036280.1 L-ascorbate oxidase [Cucumis melo var. makuwa] >TYK12674.1 L-ascorbate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 1136.7 bits (2939), Expect = 0.0e+00
Identity = 544/593 (91.74%), Postives = 557/593 (93.93%), Query Frame = 0


Query: 12  MASLLQIRQYLLILLCFFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 71
           MASLLQ +QY L+LL  FLFLLQS TAESRTL YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 72  GPTIYAHQGDTVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGV 131
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQ              IGTPWSDGTEGV
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQ--------------IGTPWSDGTEGV 120

Query: 132 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRN 191
           TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPFSYD+DRN
Sbjct: 121 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRN 180

Query: 192 LILTDWYHKSSQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSS 251
           LILTDWYHKSS EHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS   S TCNSS
Sbjct: 181 LILTDWYHKSSNEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS---SGTCNSS 240

Query: 252 NPECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIY 311
           + ECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVNNLFIY
Sbjct: 241 STECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIY 300

Query: 312 SGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAW 371
           SGETYSVLIKADQDPSRNYWITTNVVSR RTTPPGLAIFNYYPNHQ K PPTTPPA PAW
Sbjct: 301 SGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAW 360

Query: 372 NDVAPRMAQSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 431
           NDV PR+AQS AIKARQGF+HAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY
Sbjct: 361 NDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 420

Query: 432 LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNAN 491
           LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVD+ILQNAN
Sbjct: 421 LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNAN 480

Query: 492 MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALR 551
           MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIY DPSKYNLENPIMKNTVPLHPYGWTALR
Sbjct: 481 MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALR 540

Query: 552 FVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGESRRFLRP 605
           FVADNPGVWAFHCHIDAHFFMGMGVVF EGI++V KLPTSIMGCGES+RFLRP
Sbjct: 541 FVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of ClCG01G018980 vs. NCBI nr
Match: XP_004143482.1 (L-ascorbate oxidase [Cucumis sativus] >KGN48772.1 hypothetical protein Csa_003073 [Cucumis sativus])

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 538/593 (90.73%), Postives = 553/593 (93.25%), Query Frame = 0


Query: 12  MASLLQIRQYLLILLCFFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 71
           MAS LQ  +YLLILL F  FLLQS TAESRTL YKWEVKSELKSPDCFKK+T+TINGRSP
Sbjct: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60

Query: 72  GPTIYAHQGDTVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGV 131
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQ              IGTPWSDGTEGV
Sbjct: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQ--------------IGTPWSDGTEGV 120

Query: 132 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRN 191
           TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPFSYD DRN
Sbjct: 121 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRN 180

Query: 192 LILTDWYHKSSQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSS 251
           LILTDWYHKSS EHA GLATPGSGF WVGEPDSLLIQGRGRFNCS+LGSS   S TCNSS
Sbjct: 181 LILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSS---SGTCNSS 240

Query: 252 NPECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIY 311
           + ECVL+PLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIY
Sbjct: 241 STECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIY 300

Query: 312 SGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAW 371
           SGETYSVLIKADQDPSRNYWITTNVVSR RTTPPGLAIFNYYPNHQ +LPPTTPPA PAW
Sbjct: 301 SGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAW 360

Query: 372 NDVAPRMAQSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 431
           NDV PR+AQS AIKARQGF+H PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY
Sbjct: 361 NDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 420

Query: 432 LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNAN 491
           LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAI+RLDFNSTVDIILQNAN
Sbjct: 421 LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNAN 480

Query: 492 MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALR 551
           MMSPNNSETHPWHLHGHDFWVLGYGEGKFD+YKDPSKYNLENPIMKNTVPLHPYGWTALR
Sbjct: 481 MMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALR 540

Query: 552 FVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGESRRFLRP 605
           FVADNPGVWAFHCHIDAHFFMGMGVVF EGI++V KLPTSIMGCGES+RFLRP
Sbjct: 541 FVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of ClCG01G018980 vs. NCBI nr
Match: KAG6594863.1 (L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026826.1 L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 523/593 (88.20%), Postives = 550/593 (92.75%), Query Frame = 0


Query: 12  MASLLQIRQYLLILLCFFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 71
           M SLLQ RQYLLILLCFF+FL +S TAESRTL YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 72  GPTIYAHQGDTVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGV 131
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQ              IGTPWSDGTEGV
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQ--------------IGTPWSDGTEGV 120

Query: 132 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRN 191
           TQCP+LPGETF+YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPF+YD+DRN
Sbjct: 121 TQCPVLPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRN 180

Query: 192 LILTDWYHKSSQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSS 251
           LILTDWYHKSS EH+AGLA  GS FTWVGEPDSLLIQGRGRFNCS+LG    ASATCNSS
Sbjct: 181 LILTDWYHKSSHEHSAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLG----ASATCNSS 240

Query: 252 NPECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIY 311
           +P+CVLYP+TVVPG+TYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+NLFIY
Sbjct: 241 SPDCVLYPITVVPGRTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIY 300

Query: 312 SGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAW 371
           SGETYSVLIKADQ+PSRNYWITTNVVSR  TTP GLAIFNYYPNHQRK PPTTPPA PAW
Sbjct: 301 SGETYSVLIKADQNPSRNYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAW 360

Query: 372 NDVAPRMAQSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 431
           N VAPR+AQSHAIKARQGF+H PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY
Sbjct: 361 NAVAPRLAQSHAIKARQGFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 420

Query: 432 LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNAN 491
           LIALK+NLLHTFSQ KPPTGYDFKNY+IF P PNPEATVSDAI+RLDFNSTVDIILQNAN
Sbjct: 421 LIALKNNLLHTFSQAKPPTGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNAN 480

Query: 492 MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALR 551
           MM+ N SETHPWHLHGHDFWVLGYGEGKFD++KDPSKYNL NPIMKNTVPLHP+GWTALR
Sbjct: 481 MMALNTSETHPWHLHGHDFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALR 540

Query: 552 FVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGESRRFLRP 605
           FVADNPGVWAFHCHIDAHFFMGMGVVF EGI+KVQKLPTSIMGCGES+RF+RP
Sbjct: 541 FVADNPGVWAFHCHIDAHFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 575

[Close]

BLAST of ClCG01G018980 vs. NCBI nr
Match: XP_023003749.1 (L-ascorbate oxidase [Cucurbita maxima])

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 522/593 (88.03%), Postives = 550/593 (92.75%), Query Frame = 0


Query: 12  MASLLQIRQYLLILLCFFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 71
           M SLLQ RQYLLILLCFF+FL +S TAESRTL Y+WEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MGSLLQRRQYLLILLCFFVFLWESRTAESRTLYYRWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 72  GPTIYAHQGDTVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGV 131
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQ              IGTPWSDGTEGV
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQ--------------IGTPWSDGTEGV 120

Query: 132 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRN 191
           TQCP+LPGETF+YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPF+YD+DRN
Sbjct: 121 TQCPVLPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRN 180

Query: 192 LILTDWYHKSSQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSS 251
           LILTDWYHKSS EH+AGLA PGS F WVGEPDSLLIQGRGRFNCS LG    ASATCNSS
Sbjct: 181 LILTDWYHKSSHEHSAGLAAPGSAFNWVGEPDSLLIQGRGRFNCS-LG----ASATCNSS 240

Query: 252 NPECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIY 311
           +P+CVLYP+TVVPG+TYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+NLFIY
Sbjct: 241 SPDCVLYPITVVPGRTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIY 300

Query: 312 SGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAW 371
           SGETYSVLIKADQ+PSRNYWITTNVVSR  TTP GLAIFNYYPNHQRK PPTTPPA PAW
Sbjct: 301 SGETYSVLIKADQNPSRNYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAW 360

Query: 372 NDVAPRMAQSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 431
           N VAPR+AQSHAIKARQGF+H PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY
Sbjct: 361 NAVAPRLAQSHAIKARQGFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 420

Query: 432 LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNAN 491
           LIALK+NLLHTFSQ KPPTGYDFKNY+IF+P PNPEATVSDAI+RLDFNSTVDIILQNAN
Sbjct: 421 LIALKNNLLHTFSQAKPPTGYDFKNYNIFDPPPNPEATVSDAIYRLDFNSTVDIILQNAN 480

Query: 492 MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALR 551
           MM+ N SETHPWHLHGHDFWVLGYGEGKFD++KDPSKYNL NPIMKNTVPLHP+GWTALR
Sbjct: 481 MMALNTSETHPWHLHGHDFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALR 540

Query: 552 FVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGESRRFLRP 605
           FVADNPGVWAFHCHIDAHFFMGMGVVF EGI+KVQKLPTSIMGCGES+RF+RP
Sbjct: 541 FVADNPGVWAFHCHIDAHFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 574

[Close]

BLAST of ClCG01G018980 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 2.7e-170
Identity = 289/579 (49.91%), Postives = 377/579 (65.11%), Query Frame = 0


Query: 21  YLLILLCFFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQG 80
           +L++ + F   +  S     +   YKW+V+    SPDC + + M ING  PGPTI A+ G
Sbjct: 17  FLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEFPGPTIRANAG 76

Query: 81  DTVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGVTQCPILPGE 140
           D V+VEL N L TE V IHWHGI Q               GTPW+DGT  ++QC I PGE
Sbjct: 77  DIVVVELTNKLHTEGVVIHWHGILQ--------------RGTPWADGTASISQCAINPGE 136

Query: 141 TFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRNLILTDWYHK 200
           TF Y+FVVD+ GTY YH H GMQR  GLYGS++V  PEG++EPF YD + NL+L+DW+H+
Sbjct: 137 TFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQ 196

Query: 201 SSQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSSNPE-CVLYP 260
           S  +   GL++      W+GEP S+LI G+G+F+CS+    +     C  S  E C  + 
Sbjct: 197 SVHKQEVGLSS--KPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFI 256

Query: 261 LTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVL 320
           L V P KTYR+R++S T+L++L+F I  H + VVEADG+YV+PF  +++ IYSGE+YSVL
Sbjct: 257 LHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVL 316

Query: 321 IKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAWNDVAPRMA 380
           I  DQ+P  NYW++  V +R   TPPGL + NY PN   KLP + PP  P W D      
Sbjct: 317 ITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKN 376

Query: 381 QSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNL 440
            +  I A  G    PP   ++ + LLNTQN +NG  +W++NNVS  +P TPYL A+K  L
Sbjct: 377 FTFRIFAAMG-SPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRL 436

Query: 441 LHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSE 500
              F+Q  PP  +   NYDI NP PNPE T  + +++ +   TVD+ILQNANM++PN SE
Sbjct: 437 NTAFNQNPPPETFPL-NYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 496

Query: 501 THPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGV 560
            HPWHLHGHDFWVLGYGEGKF   +D  K NL+NP ++NTV + PYGWTA+RFVADNPGV
Sbjct: 497 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 556

Query: 561 WAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGES 599
           WAFHCHI+ H  MGMGVVFAEG+  V  +P   + CG +
Sbjct: 557 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGST 577

[Close]

BLAST of ClCG01G018980 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 596.7 bits (1537), Expect = 3.0e-169
Identity = 292/583 (50.09%), Postives = 384/583 (65.87%), Query Frame = 0


Query: 23  LILLCFFLFLLQSPTAE-SRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGD 82
           LIL  F L L    ++E S+   YKWEV+    +PDC + + M ING+ PGPTI A+ GD
Sbjct: 13  LILFFFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRANAGD 72

Query: 83  TVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGVTQCPILPGET 142
           TV+VEL N L TE V IHWHGI Q               GTPW+DGT  ++QC I PGET
Sbjct: 73  TVVVELINKLHTEGVVIHWHGILQ--------------RGTPWADGTASISQCAINPGET 132

Query: 143 FRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRNLILTDWYHKS 202
           F Y F VD PGT+ YH H GMQR  GLYGS++V  P+G+ EPF YD + NL+L+DW+H+S
Sbjct: 133 FFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQS 192

Query: 203 SQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLG--SSSLASATCNSSNPECVLYP 262
             +   GL++      W+GEP ++L+ GRG+F+CS+     S+L       S P C  Y 
Sbjct: 193 IHKQEVGLSS--KPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEP-CAPYI 252

Query: 263 LTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVL 322
             V+P KTYR+R++S T+L+AL+F I  H + VVEADG+YV+PF  +++ IYSGE+YSVL
Sbjct: 253 FHVMPKKTYRIRIASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVL 312

Query: 323 IKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAWNDVAPRMA 382
           I  DQ+PS NYW++     R   TPPGL + NY PN   KLP + PP  PAW+D      
Sbjct: 313 ITTDQNPSENYWVSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKN 372

Query: 383 QSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNL 442
            ++ I A  G    PP  +++ I LLNTQN +NG  +W++N+VS  +P TPYL A+K NL
Sbjct: 373 FTYRITAAMG-SPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNL 432

Query: 443 LHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSE 502
           LH F Q  PP  +  ++YDI  P  N +  + + +++      VD+ILQNANMM  N SE
Sbjct: 433 LHAFDQNPPPEVFP-EDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSE 492

Query: 503 THPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGV 562
            HPWHLHGHDFWVLGYG+GKF   ++ S  NL+NP ++NTV + PYGWTA+RFVADNPGV
Sbjct: 493 IHPWHLHGHDFWVLGYGDGKFTA-EEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 552

Query: 563 WAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGESRRFL 603
           WAFHCHI+ H  MGMGVVFAEG++KV ++PT  + CG + + L
Sbjct: 553 WAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTAKSL 575

[Close]

BLAST of ClCG01G018980 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 8.7e-169
Identity = 281/559 (50.27%), Postives = 371/559 (66.37%), Query Frame = 0


Query: 45  YKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSLGTENVAIHWHGIR 104
           YKWEV+    +P+C + + M ING+ PGPTI A+ GD+V+VEL N L TE V IHWHGI 
Sbjct: 6   YKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIHWHGIL 65

Query: 105 QVPQISSHSNYLDDFIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQR 164
           Q               GTPW+DGT  ++QC I PGETF Y F VD PGT+ YH H GMQR
Sbjct: 66  Q--------------RGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQR 125

Query: 165 EDGLYGSIVVWLPEGQTEPFSYDYDRNLILTDWYHKSSQEHAAGLATPGSGFTWVGEPDS 224
             GLYGS++V  P+G+ EPF YD + NL+L+DW+H+S  +   GL++      W+GEP +
Sbjct: 126 SAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS--KPIRWIGEPQT 185

Query: 225 LLIQGRGRFNCSVLGSSSLASATCNSSNPE-CVLYPLTVVPGKTYRLRVSSLTSLSALSF 284
           +L+ GRG+F+CS+          C     E C  Y   V P KTYR+R++S T+L+AL+F
Sbjct: 186 ILLNGRGQFDCSIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNF 245

Query: 285 QIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRQRTT 344
            I  H + VVEADG+YV+PF  +++ IYSGE+YSVLI  DQ+PS NYW++    +R   T
Sbjct: 246 AIGNHQLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNT 305

Query: 345 PPGLAIFNYYPNHQRKLPPTTPPAAPAWNDVAPRMAQSHAIKARQGFVHAPPKAADKVIV 404
           PPGL + NY PN   KLP + PP  PAW+D       ++ I A  G    PP   ++ I 
Sbjct: 306 PPGLTLLNYLPNSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMG-SPKPPVKFNRRIF 365

Query: 405 LLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPA 464
           LLNTQN +NG  +W++N+VS  +P TPYL A+K+NLLH F Q  PP  +  ++YDI  P 
Sbjct: 366 LLNTQNVINGYVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFP-EDYDIDTPP 425

Query: 465 PNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIY 524
            N +  + + +++      VD+ILQNANMM  N SETHPWHLHGHDFWVLGYG+GKF   
Sbjct: 426 TNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSA- 485

Query: 525 KDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAEGID 584
           ++ S  NL+NP ++NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MGMGVVFAEG++
Sbjct: 486 EEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVE 545

Query: 585 KVQKLPTSIMGCGESRRFL 603
           KV ++PT  + CG + + L
Sbjct: 546 KVGRIPTKALACGGTAKSL 545

[Close]

BLAST of ClCG01G018980 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 3.7e-167
Identity = 281/568 (49.47%), Postives = 372/568 (65.49%), Query Frame = 0


Query: 37  TAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSLGTENV 96
           TA +    Y WEV+ +   PDC + + M +NG+ PGPTI+A  GDT++V L N L TE +
Sbjct: 15  TASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLATEGL 74

Query: 97  AIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLY 156
            IHWHGIRQ              +G+PW+DG  GVTQC I PGETF Y F VD+PGT+ Y
Sbjct: 75  VIHWHGIRQ--------------LGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFY 134

Query: 157 HAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRNLILTDWYHKSSQEHAAGLATPGSGF 216
           H HYGMQR  GLYGS+++ + +G+ EP  YD + NL+L+DW+H+       GL++     
Sbjct: 135 HGHYGMQRSAGLYGSLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSS--RPM 194

Query: 217 TWVGEPDSLLIQGRGRFNCSVLGS-SSLASATCN-SSNPECVLYPLTVVPGKTYRLRVSS 276
            W+GE  S+LI GRG+FNCS+    SS +  TC      +C    L V P KTYR+R++S
Sbjct: 195 RWIGEAQSILINGRGQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLAS 254

Query: 277 LTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITT 336
            T+L++L+F ++GH + VVEADG+Y+ PF  +++ IYSGETYSVL+  DQDPS+NY+IT 
Sbjct: 255 STALASLNFAVQGHKLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITA 314

Query: 337 NVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAWNDVAPRMAQSHAIKARQGFVHAP 396
            V  R+  TPP L + NY      +LP + PP  P WND       S  I A  G   +P
Sbjct: 315 GVRGRKPNTPPALTVLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMG-SPSP 374

Query: 397 PKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDF 456
           P+  D+ ++LLNTQN + G  +W++NNVS  +P TPYL ++K+NL   F++  PP  Y  
Sbjct: 375 PETFDERLILLNTQNLIEGFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPV 434

Query: 457 KNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLG 516
            +YDI  P  N  A   +      FN TVD+ILQNAN ++ N SE HPWHLHGHDFWVLG
Sbjct: 435 -DYDIMTPPRNRNAKQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLG 494

Query: 517 YGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGM 576
           YGEGKF    D   YNL+NP ++NTV L+PYGWTALRFV DNPGVW FHCHI+ H  MGM
Sbjct: 495 YGEGKFKPGVDEKTYNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGM 554

Query: 577 GVVFAEGIDKVQKLPTSIMGCGESRRFL 603
           GVVFAEG++++ K+P   +GCG +++FL
Sbjct: 555 GVVFAEGLNRIGKVPDEALGCGLTKQFL 564

[Close]

BLAST of ClCG01G018980 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 2.9e-164
Identity = 296/599 (49.42%), Postives = 388/599 (64.77%), Query Frame = 0


Query: 12  MASLLQIRQYLLILLCFFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 71
           MASL  +  +LL L+   L   +S  A ++T  +KW+V+    SPD  + + M ING+ P
Sbjct: 1   MASLGFLFFFLLPLILLELSSSRSVMA-AKTRHFKWDVEYIHWSPDGEESVVMGINGQFP 60

Query: 72  GPTIYAHQGDTVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGV 131
           GPTI A  GDTV V L N L TE V IHWHGIRQ              IGTPW+DGT  +
Sbjct: 61  GPTIRAKAGDTVAVHLTNKLHTEGVVIHWHGIRQ--------------IGTPWADGTAAI 120

Query: 132 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRN 191
           +QC I PGETF Y+F VD+ GTY YH HYGMQR  GLYGS++V + EG+ EPF YD + N
Sbjct: 121 SQCAINPGETFLYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFN 180

Query: 192 LILTDWYHKSSQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSS 251
           L+L+DW+HK S E    L++  +   W+GEP +LL+ GRG++NC      SLA+      
Sbjct: 181 LLLSDWWHKGSHEQEVDLSS--NPLRWIGEPQTLLLNGRGQYNC------SLAARFSKPP 240

Query: 252 NPECVL--------YPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPF 311
            P+C L          L V P K YRLRV+S T+L +LS  I GH M VVEADG+YV+PF
Sbjct: 241 LPQCKLRGGEQYAPQILRVRPNKIYRLRVASTTALGSLSLAIGGHKMVVVEADGNYVQPF 300

Query: 312 QVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPT 371
            V ++ IYSGE+YSVL K DQDP++NYWI+ NV  R+  TP GL + NY PN   K P  
Sbjct: 301 SVQDMDIYSGESYSVLFKTDQDPTKNYWISINVRGREPKTPQGLTLLNYLPNSASKFPTL 360

Query: 372 TPPAAPAWNDVAPRMAQSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVS 431
            PP AP WND     + S+ I A  G    PP+   + I+LLNTQN ++G  +W++NNVS
Sbjct: 361 PPPIAPLWNDYNHSKSFSNKIFALMGSPKPPPQ-NHRRIILLNTQNKIDGYTKWAINNVS 420

Query: 432 FNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTV 491
             +P   YL ++++  ++ F  + PP  +  K+YD+   APN  +T  + ++ L FN+T+
Sbjct: 421 LVLPTQLYLGSIRYG-INAFDTKPPPDNFP-KDYDVLKQAPNSNSTYGNGVYMLKFNTTI 480

Query: 492 DIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLH 551
           DIILQNAN ++ + SE HPWHLHGHDFWVLGYGEGKF   KD  K+NL+NP ++NT  + 
Sbjct: 481 DIILQNANALAKDVSEIHPWHLHGHDFWVLGYGEGKFS-EKDVKKFNLKNPPLRNTAVIF 540

Query: 552 PYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGESRRFL 603
           P+GWTALRFV DNPGVWAFHCHI+ H  MGMGV+FAEG+  V+K+P   + CG + + L
Sbjct: 541 PFGWTALRFVTDNPGVWAFHCHIEPHLHMGMGVIFAEGVHLVKKIPKEALACGLTGKML 572

[Close]

BLAST of ClCG01G018980 vs. ExPASy TrEMBL
Match: A0A5A7SYW4 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002620 PE=3 SV=1)

HSP 1 Score: 1136.7 bits (2939), Expect = 0.0e+00
Identity = 544/593 (91.74%), Postives = 557/593 (93.93%), Query Frame = 0


Query: 12  MASLLQIRQYLLILLCFFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 71
           MASLLQ +QY L+LL  FLFLLQS TAESRTL YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 72  GPTIYAHQGDTVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGV 131
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQ              IGTPWSDGTEGV
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQ--------------IGTPWSDGTEGV 120

Query: 132 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRN 191
           TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPFSYD+DRN
Sbjct: 121 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRN 180

Query: 192 LILTDWYHKSSQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSS 251
           LILTDWYHKSS EHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS   S TCNSS
Sbjct: 181 LILTDWYHKSSNEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS---SGTCNSS 240

Query: 252 NPECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIY 311
           + ECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVNNLFIY
Sbjct: 241 STECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIY 300

Query: 312 SGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAW 371
           SGETYSVLIKADQDPSRNYWITTNVVSR RTTPPGLAIFNYYPNHQ K PPTTPPA PAW
Sbjct: 301 SGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAW 360

Query: 372 NDVAPRMAQSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 431
           NDV PR+AQS AIKARQGF+HAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY
Sbjct: 361 NDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 420

Query: 432 LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNAN 491
           LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVD+ILQNAN
Sbjct: 421 LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNAN 480

Query: 492 MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALR 551
           MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIY DPSKYNLENPIMKNTVPLHPYGWTALR
Sbjct: 481 MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALR 540

Query: 552 FVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGESRRFLRP 605
           FVADNPGVWAFHCHIDAHFFMGMGVVF EGI++V KLPTSIMGCGES+RFLRP
Sbjct: 541 FVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of ClCG01G018980 vs. ExPASy TrEMBL
Match: A0A1S3B151 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103484994 PE=3 SV=1)

HSP 1 Score: 1136.7 bits (2939), Expect = 0.0e+00
Identity = 544/593 (91.74%), Postives = 557/593 (93.93%), Query Frame = 0


Query: 12  MASLLQIRQYLLILLCFFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 71
           MASLLQ +QY L+LL  FLFLLQS TAESRTL YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 72  GPTIYAHQGDTVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGV 131
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQ              IGTPWSDGTEGV
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQ--------------IGTPWSDGTEGV 120

Query: 132 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRN 191
           TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPFSYD+DRN
Sbjct: 121 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRN 180

Query: 192 LILTDWYHKSSQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSS 251
           LILTDWYHKSS EHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS   S TCNSS
Sbjct: 181 LILTDWYHKSSNEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS---SGTCNSS 240

Query: 252 NPECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIY 311
           + ECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVNNLFIY
Sbjct: 241 STECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIY 300

Query: 312 SGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAW 371
           SGETYSVLIKADQDPSRNYWITTNVVSR RTTPPGLAIFNYYPNHQ K PPTTPPA PAW
Sbjct: 301 SGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAW 360

Query: 372 NDVAPRMAQSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 431
           NDV PR+AQS AIKARQGF+HAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY
Sbjct: 361 NDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 420

Query: 432 LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNAN 491
           LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVD+ILQNAN
Sbjct: 421 LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNAN 480

Query: 492 MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALR 551
           MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIY DPSKYNLENPIMKNTVPLHPYGWTALR
Sbjct: 481 MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALR 540

Query: 552 FVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGESRRFLRP 605
           FVADNPGVWAFHCHIDAHFFMGMGVVF EGI++V KLPTSIMGCGES+RFLRP
Sbjct: 541 FVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of ClCG01G018980 vs. ExPASy TrEMBL
Match: A0A0A0KK62 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_6G500640 PE=3 SV=1)

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 538/593 (90.73%), Postives = 553/593 (93.25%), Query Frame = 0


Query: 12  MASLLQIRQYLLILLCFFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 71
           MAS LQ  +YLLILL F  FLLQS TAESRTL YKWEVKSELKSPDCFKK+T+TINGRSP
Sbjct: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60

Query: 72  GPTIYAHQGDTVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGV 131
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQ              IGTPWSDGTEGV
Sbjct: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQ--------------IGTPWSDGTEGV 120

Query: 132 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRN 191
           TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPFSYD DRN
Sbjct: 121 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRN 180

Query: 192 LILTDWYHKSSQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSS 251
           LILTDWYHKSS EHA GLATPGSGF WVGEPDSLLIQGRGRFNCS+LGSS   S TCNSS
Sbjct: 181 LILTDWYHKSSHEHATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSS---SGTCNSS 240

Query: 252 NPECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIY 311
           + ECVL+PLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIY
Sbjct: 241 STECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIY 300

Query: 312 SGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAW 371
           SGETYSVLIKADQDPSRNYWITTNVVSR RTTPPGLAIFNYYPNHQ +LPPTTPPA PAW
Sbjct: 301 SGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAW 360

Query: 372 NDVAPRMAQSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 431
           NDV PR+AQS AIKARQGF+H PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY
Sbjct: 361 NDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 420

Query: 432 LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNAN 491
           LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAI+RLDFNSTVDIILQNAN
Sbjct: 421 LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNAN 480

Query: 492 MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALR 551
           MMSPNNSETHPWHLHGHDFWVLGYGEGKFD+YKDPSKYNLENPIMKNTVPLHPYGWTALR
Sbjct: 481 MMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALR 540

Query: 552 FVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGESRRFLRP 605
           FVADNPGVWAFHCHIDAHFFMGMGVVF EGI++V KLPTSIMGCGES+RFLRP
Sbjct: 541 FVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of ClCG01G018980 vs. ExPASy TrEMBL
Match: A0A6J1GE53 (L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111453168 PE=3 SV=1)

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 519/593 (87.52%), Postives = 542/593 (91.40%), Query Frame = 0


Query: 12  MASLLQIRQYLLILLCFFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 71
           MA LLQ  QYLLIL CFF+FL  S  AESRTL YKWEVK E++SPDCFKKLT+TING SP
Sbjct: 1   MAPLLQSTQYLLILFCFFVFLSASLVAESRTLFYKWEVKYEIRSPDCFKKLTITINGGSP 60

Query: 72  GPTIYAHQGDTVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGV 131
           GPTIYAHQGD VIVELKNSL +ENVA+HWHGIRQ              IGTPWSDGTEGV
Sbjct: 61  GPTIYAHQGDNVIVELKNSLASENVAVHWHGIRQ--------------IGTPWSDGTEGV 120

Query: 132 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRN 191
           TQCP+LPGETF+YQFVVDR GTYLYHAHYGMQR+DGLYGSIVVWLPEGQ+EPFSYD+DRN
Sbjct: 121 TQCPVLPGETFKYQFVVDRAGTYLYHAHYGMQRQDGLYGSIVVWLPEGQSEPFSYDHDRN 180

Query: 192 LILTDWYHKSSQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSS 251
           LILTDWYHKSS EHA GLATP  GF WVGEPDSLLIQGRGRF CS+LGSS LASATCNSS
Sbjct: 181 LILTDWYHKSSHEHATGLATPDPGFQWVGEPDSLLIQGRGRFKCSLLGSSGLASATCNSS 240

Query: 252 NPECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIY 311
           +P+CV YPLTVVPGKTYRLRVSSLTSLSALSFQIE HTMTVVEADGHYVEPFQVNNLFIY
Sbjct: 241 SPQCVAYPLTVVPGKTYRLRVSSLTSLSALSFQIESHTMTVVEADGHYVEPFQVNNLFIY 300

Query: 312 SGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAW 371
           SGETYSVLIKADQDPSRNYW TTNVVSR RTTPPGLAIFNYYPNH+RKLPPTTPPA PAW
Sbjct: 301 SGETYSVLIKADQDPSRNYWATTNVVSRPRTTPPGLAIFNYYPNHRRKLPPTTPPAGPAW 360

Query: 372 NDVAPRMAQSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 431
           NDVAPR+AQSHAIKARQGF+H PPK ADKVIVLLNTQNTV GRRRWSLNNVSFNMPHTPY
Sbjct: 361 NDVAPRLAQSHAIKARQGFIHPPPKIADKVIVLLNTQNTVKGRRRWSLNNVSFNMPHTPY 420

Query: 432 LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNAN 491
           LIALKHNLLHTFSQ KPPTGYDFKNYDIFNPAPN EAT SDAIFRL+F STVDIILQNAN
Sbjct: 421 LIALKHNLLHTFSQAKPPTGYDFKNYDIFNPAPNQEATESDAIFRLEFKSTVDIILQNAN 480

Query: 492 MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALR 551
           MMSPN+S+THPWHLHGHDFWVLGYGEGKFDIY+DPSKYNLENPIMKNTV LHPYGWTALR
Sbjct: 481 MMSPNSSDTHPWHLHGHDFWVLGYGEGKFDIYRDPSKYNLENPIMKNTVALHPYGWTALR 540

Query: 552 FVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGESRRFLRP 605
           FVADNPGVWAFHCHIDAHFFMGMGVVF EGID+V  LPTSIMGCGES+RFLRP
Sbjct: 541 FVADNPGVWAFHCHIDAHFFMGMGVVFEEGIDQVGNLPTSIMGCGESKRFLRP 579

[Close]

BLAST of ClCG01G018980 vs. ExPASy TrEMBL
Match: A0A6J1KU77 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111497239 PE=3 SV=1)

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 522/593 (88.03%), Postives = 550/593 (92.75%), Query Frame = 0


Query: 12  MASLLQIRQYLLILLCFFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 71
           M SLLQ RQYLLILLCFF+FL +S TAESRTL Y+WEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MGSLLQRRQYLLILLCFFVFLWESRTAESRTLYYRWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 72  GPTIYAHQGDTVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGV 131
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQ              IGTPWSDGTEGV
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQ--------------IGTPWSDGTEGV 120

Query: 132 TQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRN 191
           TQCP+LPGETF+YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPF+YD+DRN
Sbjct: 121 TQCPVLPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRN 180

Query: 192 LILTDWYHKSSQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSS 251
           LILTDWYHKSS EH+AGLA PGS F WVGEPDSLLIQGRGRFNCS LG    ASATCNSS
Sbjct: 181 LILTDWYHKSSHEHSAGLAAPGSAFNWVGEPDSLLIQGRGRFNCS-LG----ASATCNSS 240

Query: 252 NPECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIY 311
           +P+CVLYP+TVVPG+TYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+NLFIY
Sbjct: 241 SPDCVLYPITVVPGRTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIY 300

Query: 312 SGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAW 371
           SGETYSVLIKADQ+PSRNYWITTNVVSR  TTP GLAIFNYYPNHQRK PPTTPPA PAW
Sbjct: 301 SGETYSVLIKADQNPSRNYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAW 360

Query: 372 NDVAPRMAQSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 431
           N VAPR+AQSHAIKARQGF+H PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY
Sbjct: 361 NAVAPRLAQSHAIKARQGFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPY 420

Query: 432 LIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNAN 491
           LIALK+NLLHTFSQ KPPTGYDFKNY+IF+P PNPEATVSDAI+RLDFNSTVDIILQNAN
Sbjct: 421 LIALKNNLLHTFSQAKPPTGYDFKNYNIFDPPPNPEATVSDAIYRLDFNSTVDIILQNAN 480

Query: 492 MMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALR 551
           MM+ N SETHPWHLHGHDFWVLGYGEGKFD++KDPSKYNL NPIMKNTVPLHP+GWTALR
Sbjct: 481 MMALNTSETHPWHLHGHDFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALR 540

Query: 552 FVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGESRRFLRP 605
           FVADNPGVWAFHCHIDAHFFMGMGVVF EGI+KVQKLPTSIMGCGES+RF+RP
Sbjct: 541 FVADNPGVWAFHCHIDAHFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 574

[Close]

BLAST of ClCG01G018980 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 805.1 bits (2078), Expect = 4.0e-233
Identity = 384/588 (65.31%), Postives = 449/588 (76.36%), Query Frame = 0


Query: 18  IRQYLLILLCFFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYA 77
           +  + L++LCF      S   + +   +KWEVK E KSPDCF+KL +TING+ PGPTI A
Sbjct: 11  VHVFNLMVLCFIALFFSSVLCQGKIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKA 70

Query: 78  HQGDTVIVELKNSLGTENVAIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGVTQCPIL 137
            QGDT++VELKNS  TENVA+HWHGIRQ              IGTPW DG EGVTQCPIL
Sbjct: 71  QQGDTIVVELKNSFMTENVAVHWHGIRQ--------------IGTPWFDGVEGVTQCPIL 130

Query: 138 PGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRNLILTDW 197
           PGE F YQFVVDRPGTY+YH+HYGMQRE GL G I V  P  + EPF+YDYDRN +LTDW
Sbjct: 131 PGEVFIYQFVVDRPGTYMYHSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDW 190

Query: 198 YHKSSQEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCS--VLGSSSLASATCNSSNPEC 257
           YHKS  E A GLA+    F WVGEP SL+IQGRGRFNCS  +    SL S  CN SN +C
Sbjct: 191 YHKSMSEKATGLAS--IPFKWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGVCNVSNADC 250

Query: 258 VLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGET 317
             + LTV+PGKTYRLR+ SLT+LSALSFQIEGH +TVVEADGHYVEPF V NLF+YSGET
Sbjct: 251 SRFILTVIPGKTYRLRIGSLTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGET 310

Query: 318 YSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPA--APAWND 377
           YSVL+KADQ+P RNYWIT+++VSR  TTPP  A+ NYYPNH R+ PPT+  +   P WND
Sbjct: 311 YSVLLKADQNPRRNYWITSSIVSRPATTPPATAVLNYYPNHPRRRPPTSESSNIVPEWND 370

Query: 378 VAPRMAQSHAIKARQGFVHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLI 437
              R+AQS AIKAR+GF+HA P+ +DKVIVLLNTQN VNG RRWS+NNVS++ P TPYLI
Sbjct: 371 TRSRLAQSLAIKARRGFIHALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLI 430

Query: 438 ALKHNLLHTFS-QEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANM 497
           ALK NL + F  +   P  YD +NYDIF    N  AT SD I+RL FNSTVD+ILQNAN 
Sbjct: 431 ALKQNLTNAFDWRFTAPENYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANT 490

Query: 498 MSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRF 557
           M+ NNSETHPWHLHGHDFWVLGYGEGKF+  +DP +YN  +PI KNTV + P+GWTALRF
Sbjct: 491 MNANNSETHPWHLHGHDFWVLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRF 550

Query: 558 VADNPGVWAFHCHIDAHFFMGMGVVFAEGIDKVQKLPTSIMGCGESRR 601
            ADNPGVW+FHCHI++HFFMGMG+VF  GIDKV  LP+SIMGCG+++R
Sbjct: 551 RADNPGVWSFHCHIESHFFMGMGIVFESGIDKVSSLPSSIMGCGQTKR 582

[Close]

BLAST of ClCG01G018980 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 574.7 bits (1480), Expect = 8.7e-164
Identity = 275/569 (48.33%), Postives = 370/569 (65.03%), Query Frame = 0


Query: 37  TAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSLGTENV 96
           TA +    Y WEV+ +  SPDC +   MT+NG  PGPTI A  GDT++V L N L TE +
Sbjct: 32  TASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGL 91

Query: 97  AIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLY 156
            IHWHGIRQ               G+PW+DG  GVTQC I PGETF Y F V++PGT+ Y
Sbjct: 92  VIHWHGIRQ--------------FGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFY 151

Query: 157 HAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRNLILTDWYHKSSQEHAAGLATPGSGF 216
           H HYGMQR  GLYGS++V + +G++E   YD + NL+L+DW+H++      GL++     
Sbjct: 152 HGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSS--KPM 211

Query: 217 TWVGEPDSLLIQGRGRFNCSVLG--SSSLASATCN-SSNPECVLYPLTVVPGKTYRLRVS 276
            W+GE  S+LI GRG+FNCS+    S++ +   C      +C    L V P KTYR+R+S
Sbjct: 212 RWIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLS 271

Query: 277 SLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWIT 336
           S T+L++L+  ++GH + VVEADG+Y+ PF  +++ IYSGE+YSVL+  DQDPS+NY+I+
Sbjct: 272 STTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYIS 331

Query: 337 TNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAWNDVAPRMAQSHAIKARQGFVHA 396
             V  R+  T   L I NY      KLP + PP  P W+D       S  I +  G   +
Sbjct: 332 VGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPS 391

Query: 397 PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYD 456
           PPK   K ++LLNTQN ++G  +W++NNVS   P TPYL ++K+NL   F+++ PP  Y 
Sbjct: 392 PPKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYR 451

Query: 457 FKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVL 516
             +YDI NP P P  T  + I+   FN TVD+I+QNAN++    SE HPWHLHGHDFWVL
Sbjct: 452 M-DYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVL 511

Query: 517 GYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMG 576
           GYG+GKF    D   YNL+NP ++NT  L+PYGWTA+RFV DNPGVW FHCHI+ H  MG
Sbjct: 512 GYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMG 571

Query: 577 MGVVFAEGIDKVQKLPTSIMGCGESRRFL 603
           MGVVFAEG++++ K+P   +GCG +++FL
Sbjct: 572 MGVVFAEGLNRIGKVPDEALGCGLTKQFL 582

[Close]

BLAST of ClCG01G018980 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 554.3 bits (1427), Expect = 1.2e-157
Identity = 270/571 (47.29%), Postives = 356/571 (62.35%), Query Frame = 0


Query: 37  TAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSLGTENV 96
           +A +  +   WEV+ +   PDC + + M ING+ PGPTI A  GDTVI+ + N L TE V
Sbjct: 18  SASAAVVESTWEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGV 77

Query: 97  AIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLY 156
            IHWHGIRQ               GTPW+DG  GVTQCPI PGETF Y+F+VD+ GT+ Y
Sbjct: 78  VIHWHGIRQK--------------GTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFY 137

Query: 157 HAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRNLILTDWYHKSSQEHAAGLATPGSGF 216
           H HYGMQR  GLYG ++V  P+   E   YD + NL+L+DW+H+S   HA  LA      
Sbjct: 138 HGHYGMQRSSGLYGMLIVRSPK---ERLIYDGEFNLLLSDWWHQSI--HAQELALSSRPM 197

Query: 217 TWVGEPDSLLIQGRGRFNCS---VLGSSSLASATCNSSNPECVLYPLTVVPGKTYRLRVS 276
            W+GEP SLLI GRG+FNCS                  N +C    L V P + YRLR++
Sbjct: 198 RWIGEPQSLLINGRGQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIA 257

Query: 277 SLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWIT 336
           S T+L++L+  ++GH + VVEADG+YV PF VN++ +YSGETYSVL+K +  PS+ YWI+
Sbjct: 258 STTALASLNLAVQGHQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWIS 317

Query: 337 TNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAWNDVAPRMAQSHAIKARQGFVHA 396
             V  R+  TP  L + NY    + + P   PP  P WND     + S  I A +G+   
Sbjct: 318 VGVRGREPKTPQALTVINYVDATESR-PSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKP 377

Query: 397 PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYD 456
           P K+ D+ ++LLNTQN      +WS+NNVS ++P TPYL ++++ L   +  + P     
Sbjct: 378 PEKSHDQ-LILLNTQNLYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLI 437

Query: 457 FKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVL 516
             NYDI  P PNP  T    I+   F   VD+ILQNAN++    SE HPWH+HGHDFWVL
Sbjct: 438 MDNYDIMKPPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVL 497

Query: 517 GYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMG 576
           GYGEGKF    D   +NL+NP ++NTV L+P+GWTA+RFV DNPGVW FHCHI+ H  MG
Sbjct: 498 GYGEGKFKPGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMG 557

Query: 577 MGVVFAEGIDKVQK--LPTSIMGCGESRRFL 603
           MGVVF EG+D++ K  +P   +GCG +R++L
Sbjct: 558 MGVVFVEGVDRIGKMEIPDEALGCGLTRKWL 567

[Close]

BLAST of ClCG01G018980 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 512.3 bits (1318), Expect = 5.3e-145
Identity = 250/525 (47.62%), Postives = 335/525 (63.81%), Query Frame = 0


Query: 37  TAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSLGTENV 96
           TA +    Y WEV+ +  SPDC +   MT+NG  PGPTI A  GDT++V L N L TE +
Sbjct: 32  TASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGL 91

Query: 97  AIHWHGIRQVPQISSHSNYLDDFIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLY 156
            IHWHGIRQ               G+PW+DG  GVTQC I PGETF Y F V++PGT+ Y
Sbjct: 92  VIHWHGIRQ--------------FGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFY 151

Query: 157 HAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRNLILTDWYHKSSQEHAAGLATPGSGF 216
           H HYGMQR  GLYGS++V + +G++E   YD + NL+L+DW+H++      GL++     
Sbjct: 152 HGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSS--KPM 211

Query: 217 TWVGEPDSLLIQGRGRFNCSVLG--SSSLASATCN-SSNPECVLYPLTVVPGKTYRLRVS 276
            W+GE  S+LI GRG+FNCS+    S++ +   C      +C    L V P KTYR+R+S
Sbjct: 212 RWIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLS 271

Query: 277 SLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWIT 336
           S T+L++L+  ++GH + VVEADG+Y+ PF  +++ IYSGE+YSVL+  DQDPS+NY+I+
Sbjct: 272 STTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYIS 331

Query: 337 TNVVSRQRTTPPGLAIFNYYPNHQRKLPPTTPPAAPAWNDVAPRMAQSHAIKARQGFVHA 396
             V  R+  T   L I NY      KLP + PP  P W+D       S  I +  G   +
Sbjct: 332 VGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPS 391

Query: 397 PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYD 456
           PPK   K ++LLNTQN ++G  +W++NNVS   P TPYL ++K+NL   F+++ PP  Y 
Sbjct: 392 PPKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYR 451

Query: 457 FKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVL 516
             +YDI NP P P  T  + I+   FN TVD+I+QNAN++    SE HPWHLHGHDFWVL
Sbjct: 452 M-DYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVL 511

Query: 517 GYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPG 559
           GYG+GKF    D   YNL+NP ++NT  L+PYGWTA+RFV DNPG
Sbjct: 512 GYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538

[Close]

BLAST of ClCG01G018980 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 386.0 bits (990), Expect = 5.7e-107
Identity = 187/382 (48.95%), Postives = 254/382 (66.49%), Query Frame = 0


Query: 224 SLLIQGRGRFNCSVLG--SSSLASATCN-SSNPECVLYPLTVVPGKTYRLRVSSLTSLSA 283
           S+LI GRG+FNCS+    S++ +   C      +C    L V P KTYR+R+SS T+L++
Sbjct: 12  SILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALAS 71

Query: 284 LSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRQ 343
           L+  ++GH + VVEADG+Y+ PF  +++ IYSGE+YSVL+  DQDPS+NY+I+  V  R+
Sbjct: 72  LNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRK 131

Query: 344 RTTPPGLAIFNYYPNHQRKLPPTTPPAAPAWNDVAPRMAQSHAIKARQGFVHAPPKAADK 403
             T   L I NY      KLP + PP  P W+D       S  I +  G   +PPK   K
Sbjct: 132 PNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRK 191

Query: 404 VIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIF 463
            ++LLNTQN ++G  +W++NNVS   P TPYL ++K+NL   F+++ PP  Y   +YDI 
Sbjct: 192 RLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIM 251

Query: 464 NPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKF 523
           NP P P  T  + I+   FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GKF
Sbjct: 252 NPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKF 311

Query: 524 DIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAE 583
               D   YNL+NP ++NT  L+PYGWTA+RFV DNPGVW FHCHI+ H  MGMGVVFAE
Sbjct: 312 KPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAE 371

Query: 584 GIDKVQKLPTSIMGCGESRRFL 603
           G++++ K+P   +GCG +++FL
Sbjct: 372 GLNRIGKVPDEALGCGLTKQFL 391

The following BLAST results are available for this feature:

BLAST of ClCG01G018980 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881120.1	0.0e+00	93.34	L-ascorbate oxidase [Benincasa hispida]	[more]
XP_008440636.1	0.0e+00	91.74	PREDICTED: L-ascorbate oxidase [Cucumis melo] >KAA0036280.1 L-ascorbate oxidase ...	[more]
XP_004143482.1	0.0e+00	90.73	L-ascorbate oxidase [Cucumis sativus] >KGN48772.1 hypothetical protein Csa_00307...	[more]
KAG6594863.1	0.0e+00	88.20	L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026826...	[more]
XP_023003749.1	0.0e+00	88.03	L-ascorbate oxidase [Cucurbita maxima]	[more]

BLAST of ClCG01G018980 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P14133	2.7e-170	49.91	L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
P24792	3.0e-169	50.09	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2	[more]
P37064	8.7e-169	50.27	L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1	[more]
M4DUF2	3.7e-167	49.47	L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1	[more]
Q40588	2.9e-164	49.42	L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1	[more]

BLAST of ClCG01G018980 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SYW4	0.0e+00	91.74	L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G...	[more]
A0A1S3B151	0.0e+00	91.74	L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103484994 PE=3 SV=1	[more]
A0A0A0KK62	0.0e+00	90.73	L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_6G500640 PE=3 SV=1	[more]
A0A6J1GE53	0.0e+00	87.52	L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111453168 PE=3 SV=1	[more]
A0A6J1KU77	0.0e+00	88.03	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111497239 PE=3 SV=1	[more]

BLAST of ClCG01G018980 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39830.1	4.0e-233	65.31	Cupredoxin superfamily protein	[more]
AT5G21105.1	8.7e-164	48.33	Plant L-ascorbate oxidase	[more]
AT5G21100.1	1.2e-157	47.29	Plant L-ascorbate oxidase	[more]
AT5G21105.3	5.3e-145	47.62	Plant L-ascorbate oxidase	[more]
AT5G21105.2	5.7e-107	48.95	Plant L-ascorbate oxidase	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011707	Multicopper oxidase, N-termianl	PFAM	PF07732	Cu-oxidase_3	coord: 52..175 e-value: 2.9E-32 score: 111.2
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 32..177 e-value: 8.3E-45 score: 154.7
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 184..390 e-value: 1.0E-62 score: 213.8
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 392..601 e-value: 6.2E-70 score: 237.1
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 30..203
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 395..598
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 183..381
IPR001117	Multicopper oxidase, type 1	PFAM	PF00394	Cu-oxidase	coord: 192..354 e-value: 4.3E-47 score: 160.2
IPR017760	L-ascorbate oxidase, plants	TIGRFAM	TIGR03388	TIGR03388	coord: 44..597 e-value: 7.9E-277 score: 917.2
IPR011706	Multicopper oxidase, C-terminal	PFAM	PF07731	Cu-oxidase_2	coord: 458..582 e-value: 1.2E-37 score: 128.7
IPR045087	Multicopper oxidase	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 19..599
None	No IPR available	PANTHER	PTHR11709:SF237	CUPREDOXIN SUPERFAMILY PROTEIN	coord: 19..599
IPR033138	Multicopper oxidases, conserved site	PROSITE	PS00079	MULTICOPPER_OXIDASE1	coord: 558..578
IPR002355	Multicopper oxidase, copper-binding site	PROSITE	PS00080	MULTICOPPER_OXIDASE2	coord: 563..574
IPR034267	Ascorbate oxidase, third cupredoxin domain	CDD	cd13893	CuRO_3_AAO	coord: 398..589 e-value: 3.19742E-84 score: 258.119

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG01G018980.2	ClCG01G018980.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1900367	positive regulation of defense response to insect
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0008447	L-ascorbate oxidase activity
molecular_function	GO:0016491	oxidoreductase activity