ClCG01G012750 (gene) Watermelon (Charleston Gray) v2.5

Overview
NameClCG01G012750
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
DescriptionSplicing factor U2af small subunit B-like
LocationCG_Chr01: 25613876 .. 25615599 (-)
RNA-Seq ExpressionClCG01G012750
SyntenyClCG01G012750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGCATTTGGCATCCATATTCGGGACGGAGAAGGATAGGGTTAATTGTCCTTTCTACTTCAAGATCGGGGCCTGTCGGCACGGTGATAGGTGTTCACGTTTACACACGAAGCCTAGTATAAGCCCCACCCTCTTGCTTTCTAATATGTACCAGAGACCCGACATGATTACTCCTGGTGTCGATGCGCAAGGGAATCCAATTGACCCTCGCAAGATCCAGGACCATTTTGAGGTAGATCTTGTTCTGAATTCCGTTCTTTTCCTTTTCAATCTTCTAATATTTGCTAATACATATTTTTCGTCATGAATTTTGATATATTTTGGTGAAAGGATGGGTGCGGATCTTGGCTTTTTTGATTTATTTTATCATAATCTTGCTGTAAATTTAGAGGTGGTCTAATTTGGTTGATTTTCGAGCGATCGTGTTATCATGGATATTGTTTGTTGAATCTTTTTCGTGTCCTAATTTACCTCTTTATGGTCTTGGTGTGGCTTGTAAGCTCTGTTATTTTGAAGTTGGGTTGTTTTTTTCTTGTGGCATGTGGAAGAATTGTAGCTTAATTGTTGATCTGTGTTCGTTGCAGGAGTTCTATGAAGATCTATTCCAGGAACTGAACAAGTACGGGGAGATTGAAAGTTTGAATGTCTGTGACAATCTAGCTGACCATATGGTAATACTGTCCTCTTTGCCCATTGTTTGAAGGGAACTGATTTTACAATCAGCCTTTCAACTTTGATATATGCTATTTCATTGTAGAATTAGCTAATGATCCATCTGATGTGTTTGATTAGGTGGGCAATGTTTATGTTCAATTTCGAGAGGAGGAGCAAGCTGCAAATGCTCTTCGGAATCTTAGTGGTAGATTCTATGCTGGTCAGTTGGAGTTCCTGTCCCCTCCCTTCCCTCTCTCCTGTCCTTTATTTACCGACCATGGTGTTTATATTACTTGTATGCAGCCTTTGATTTTAAAATTGGATGAAATTGGATATGTAACATATGGAGATCTATTTCTTTGCAGGACGTCCGATCATTGTTGACTTCTCTCCAGTTACGGACTTTAGAGAAGCTACATGCAGGCAATATGAAGAAAACATGTGTAATCGTGGTGGATATTGCAATTTCATGCATTTGAAGAGGATCAGTAGGTATATTTTCTTCACCACCTCCTTCTCTTCCCTCTAAGATGCATCATTCTTCCTGTTGATTTTGTATTGCCACTTCTGCTATGTGTACTTCATTTGATAGCCAATCCTTTCCTGTCTGTCAGAGAGCTGAGGCATGAACTGTTTGCAATTTATCGTCGAAGGCGTAGTCACAGCAGGAGCAGAAGCCGCAGCCCCTATAGGCATAGGAGTTATGAAGAACGTTCTTATGGCAGGCATGGTCATAGTCGAAGGTATGATGAGAGAGATGCTTATCATGAAAGTCGGAGTAGGAGGCACAGGACTACAAGCCCTGGTCATAGAAGCAGAAGTCGTAGTCCACGAGGAAGGAAGAACCAAAGTCCAGTTAGGGAAGGGAGTGAAGAGAGGCGTGCTAAAATTGAGCAGTGGAACAGGGAAAGAGAACAAGGAAATATCGATAATAATCCCAATTCTAATGACAACAAAAATAGCCATGAGAACAGCCACAATGGTGATGTGAAGTATGCAAATCAAACTTGTGGCTATGAGGAGCAGCGGCAGCGGCAGCCTCCTGAGCAAGGCTATGGTTACTGA

mRNA sequence

ATGGCGGAGCATTTGGCATCCATATTCGGGACGGAGAAGGATAGGGTTAATTGTCCTTTCTACTTCAAGATCGGGGCCTGTCGGCACGGTGATAGGTGTTCACGTTTACACACGAAGCCTAGTATAAGCCCCACCCTCTTGCTTTCTAATATGTACCAGAGACCCGACATGATTACTCCTGGTGTCGATGCGCAAGGGAATCCAATTGACCCTCGCAAGATCCAGGACCATTTTGAGGAGTTCTATGAAGATCTATTCCAGGAACTGAACAAGTACGGGGAGATTGAAAGTTTGAATGTCTGTGACAATCTAGCTGACCATATGGTGGGCAATGTTTATGTTCAATTTCGAGAGGAGGAGCAAGCTGCAAATGCTCTTCGGAATCTTAGTGGTAGATTCTATGCTGGTCAGTTGGAGTTCCTGTCCCCTCCCTTCCCTCTCTCCTGTCCTTTATTTACCGACCATGGACGTCCGATCATTGTTGACTTCTCTCCAGTTACGGACTTTAGAGAAGCTACATGCAGGCAATATGAAGAAAACATGTGTAATCGTGGTGGATATTGCAATTTCATGCATTTGAAGAGGATCAGTAGAGAGCTGAGGCATGAACTGTTTGCAATTTATCGTCGAAGGCGTAGTCACAGCAGGAGCAGAAGCCGCAGCCCCTATAGGCATAGGAGTTATGAAGAACGTTCTTATGGCAGGCATGGTCATAGTCGAAGGTATGATGAGAGAGATGCTTATCATGAAAGTCGGAGTAGGAGGCACAGGACTACAAGCCCTGGTCATAGAAGCAGAAGTCGTAGTCCACGAGGAAGGAAGAACCAAAGTCCAGTTAGGGAAGGGAGTGAAGAGAGGCGTGCTAAAATTGAGCAGTGGAACAGGGAAAGAGAACAAGGAAATATCGATAATAATCCCAATTCTAATGACAACAAAAATAGCCATGAGAACAGCCACAATGGTGATGTGAAGTATGCAAATCAAACTTGTGGCTATGAGGAGCAGCGGCAGCGGCAGCCTCCTGAGCAAGGCTATGGTTACTGA

Coding sequence (CDS)

ATGGCGGAGCATTTGGCATCCATATTCGGGACGGAGAAGGATAGGGTTAATTGTCCTTTCTACTTCAAGATCGGGGCCTGTCGGCACGGTGATAGGTGTTCACGTTTACACACGAAGCCTAGTATAAGCCCCACCCTCTTGCTTTCTAATATGTACCAGAGACCCGACATGATTACTCCTGGTGTCGATGCGCAAGGGAATCCAATTGACCCTCGCAAGATCCAGGACCATTTTGAGGAGTTCTATGAAGATCTATTCCAGGAACTGAACAAGTACGGGGAGATTGAAAGTTTGAATGTCTGTGACAATCTAGCTGACCATATGGTGGGCAATGTTTATGTTCAATTTCGAGAGGAGGAGCAAGCTGCAAATGCTCTTCGGAATCTTAGTGGTAGATTCTATGCTGGTCAGTTGGAGTTCCTGTCCCCTCCCTTCCCTCTCTCCTGTCCTTTATTTACCGACCATGGACGTCCGATCATTGTTGACTTCTCTCCAGTTACGGACTTTAGAGAAGCTACATGCAGGCAATATGAAGAAAACATGTGTAATCGTGGTGGATATTGCAATTTCATGCATTTGAAGAGGATCAGTAGAGAGCTGAGGCATGAACTGTTTGCAATTTATCGTCGAAGGCGTAGTCACAGCAGGAGCAGAAGCCGCAGCCCCTATAGGCATAGGAGTTATGAAGAACGTTCTTATGGCAGGCATGGTCATAGTCGAAGGTATGATGAGAGAGATGCTTATCATGAAAGTCGGAGTAGGAGGCACAGGACTACAAGCCCTGGTCATAGAAGCAGAAGTCGTAGTCCACGAGGAAGGAAGAACCAAAGTCCAGTTAGGGAAGGGAGTGAAGAGAGGCGTGCTAAAATTGAGCAGTGGAACAGGGAAAGAGAACAAGGAAATATCGATAATAATCCCAATTCTAATGACAACAAAAATAGCCATGAGAACAGCCACAATGGTGATGTGAAGTATGCAAATCAAACTTGTGGCTATGAGGAGCAGCGGCAGCGGCAGCCTCCTGAGCAAGGCTATGGTTACTGA

Protein sequence

MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEEQAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISRELRHELFAIYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSRRYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKNQSPVREGSEERRAKIEQWNREREQGNIDNNPNSNDNKNSHENSHNGDVKYANQTCGYEEQRQRQPPEQGYGY
Homology
BLAST of ClCG01G012750 vs. NCBI nr
Match: XP_004139466.1 (splicing factor U2af small subunit B [Cucumis sativus] >XP_011654262.1 splicing factor U2af small subunit B [Cucumis sativus] >KGN64894.1 hypothetical protein Csa_022738 [Cucumis sativus])

HSP 1 Score: 606.7 bits (1563), Expect = 1.3e-169
Identity = 309/347 (89.05%), Postives = 320/347 (92.22%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR IQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
           QAANALRNLSGRFYA                    GRPIIVDFSPVTDFREATCRQYEEN
Sbjct: 121 QAANALRNLSGRFYA--------------------GRPIIVDFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR 240
           MCNRGGYCNFMHLKRI RELRHELFA+YRRR SHSRSRSRSPYRHRSYEERSYG+HGHSR
Sbjct: 181 MCNRGGYCNFMHLKRIGRELRHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSR 240

Query: 241 RYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKNQSPVREGSEERRAKIEQWNREREQG 300
           RYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKN+SPVREGSEERRAKIEQWN+EREQG
Sbjct: 241 RYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQG 300

Query: 301 NIDNNPNSNDNKNSHENSHNGDVKYANQTCGYEEQRQRQPPEQGYGY 348
           N DNN NS+DN+N+HE S++ +VKYANQTCGYEEQ+QRQPPEQGYGY
Sbjct: 301 N-DNNANSDDNRNNHEKSYDSEVKYANQTCGYEEQQQRQPPEQGYGY 326

BLAST of ClCG01G012750 vs. NCBI nr
Match: XP_008461981.1 (PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >XP_008461988.1 PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >KAA0065889.1 splicing factor U2af small subunit B-like [Cucumis melo var. makuwa] >TYK15128.1 splicing factor U2af small subunit B-like [Cucumis melo var. makuwa])

HSP 1 Score: 605.9 bits (1561), Expect = 2.2e-169
Identity = 310/347 (89.34%), Postives = 319/347 (91.93%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
           QAANALRNLSGRFYA                    GRPIIVDFSPVTDFREATCRQYEEN
Sbjct: 121 QAANALRNLSGRFYA--------------------GRPIIVDFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR 240
           MCNRGGYCNFMHLKRI RELRHELFA+YRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR
Sbjct: 181 MCNRGGYCNFMHLKRIGRELRHELFAMYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR 240

Query: 241 RYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKNQSPVREGSEERRAKIEQWNREREQG 300
           RYDERDAYHE+RSRR RTTSPGHRSRSRSPRGRKN+SP REGSEERRAKIEQWNREREQG
Sbjct: 241 RYDERDAYHENRSRRRRTTSPGHRSRSRSPRGRKNRSPAREGSEERRAKIEQWNREREQG 300

Query: 301 NIDNNPNSNDNKNSHENSHNGDVKYANQTCGYEEQRQRQPPEQGYGY 348
           N DNN  S+DN+NSHE S++ +VKYANQTCGYEEQ+QRQPPEQGYGY
Sbjct: 301 N-DNNAKSDDNRNSHEKSYDSEVKYANQTCGYEEQQQRQPPEQGYGY 326

BLAST of ClCG01G012750 vs. NCBI nr
Match: XP_038894442.1 (splicing factor U2af small subunit B-like [Benincasa hispida] >XP_038894443.1 splicing factor U2af small subunit B-like [Benincasa hispida] >XP_038894444.1 splicing factor U2af small subunit B-like [Benincasa hispida] >XP_038894445.1 splicing factor U2af small subunit B-like [Benincasa hispida])

HSP 1 Score: 599.7 bits (1545), Expect = 1.6e-167
Identity = 311/347 (89.63%), Postives = 317/347 (91.35%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
           QAANALRNLSGRFYA                    GRPIIVDFSPVTDFREATCRQYEEN
Sbjct: 121 QAANALRNLSGRFYA--------------------GRPIIVDFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR 240
           MCNRGGYCNFMHLKRISRELRHELFAIYRRR S SRSRSRSPYRHRSYEERSYGRHGHSR
Sbjct: 181 MCNRGGYCNFMHLKRISRELRHELFAIYRRRHSRSRSRSRSPYRHRSYEERSYGRHGHSR 240

Query: 241 RYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKNQSPVREGSEERRAKIEQWNREREQG 300
           RYDERDAY+ESR  RHRTTSPGHRSRSRSPRGRKN+SPVREGSEE RAKIEQWNREREQG
Sbjct: 241 RYDERDAYYESRGMRHRTTSPGHRSRSRSPRGRKNRSPVREGSEESRAKIEQWNREREQG 300

Query: 301 NIDNNPNSNDNKNSHENSHNGDVKYANQTCGYEEQRQRQPPEQGYGY 348
           N DNN NS DNKNSHE S++GDVKY+NQTCGYEEQRQ+ PPEQGYGY
Sbjct: 301 N-DNNGNS-DNKNSHEKSYSGDVKYSNQTCGYEEQRQQLPPEQGYGY 325

BLAST of ClCG01G012750 vs. NCBI nr
Match: XP_023519661.1 (splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_023519662.1 splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_023519664.1 splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 584.3 bits (1505), Expect = 6.8e-163
Identity = 302/350 (86.29%), Postives = 316/350 (90.29%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQF+EEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEE 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
           QAANALRNLSGRFYA                    GRPIIVDFSPVTDFREATCRQYEEN
Sbjct: 121 QAANALRNLSGRFYA--------------------GRPIIVDFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR 240
           MCNRGGYCNFMHLKRISRELRHELFA Y RRRSHSRSRSRSPYRHRSYEER YGRHGHSR
Sbjct: 181 MCNRGGYCNFMHLKRISRELRHELFASYHRRRSHSRSRSRSPYRHRSYEERHYGRHGHSR 240

Query: 241 RYDERDAYHESRSRRHRTTSPGHR-SRSRSPRGRKNQSPVREGSEERRAKIEQWNREREQ 300
           RYDERDAYH+SR+RRHR+TSPGHR SRSRSPRGRKN+SPVREGSEERRAKIEQWNRE+EQ
Sbjct: 241 RYDERDAYHDSRNRRHRSTSPGHRGSRSRSPRGRKNRSPVREGSEERRAKIEQWNREKEQ 300

Query: 301 GNIDNNPNSNDNKNSHENSHNGDVKYANQTCGYEEQRQRQ--PPEQGYGY 348
               NN NS+ N+NSHE S+NGDV+Y NQTCGY++Q+Q+Q  PPEQGYGY
Sbjct: 301 QENYNNANSDGNENSHEKSYNGDVQYENQTCGYKQQQQQQQKPPEQGYGY 330

BLAST of ClCG01G012750 vs. NCBI nr
Match: XP_022964867.1 (splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022964868.1 splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022964870.1 splicing factor U2af small subunit B-like [Cucurbita moschata] >KAG7019309.1 Splicing factor U2af small subunit A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 582.4 bits (1500), Expect = 2.6e-162
Identity = 301/350 (86.00%), Postives = 316/350 (90.29%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQF+EEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEE 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
           QAANALRNLSGRFYA                    GRPIIVDFSPVTDFREATCRQYEEN
Sbjct: 121 QAANALRNLSGRFYA--------------------GRPIIVDFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR 240
           MCNRGGYCNFMHLKRISRELRHELFA Y RRRSHSRSRSRSPYRHRSYEER YGRHGHSR
Sbjct: 181 MCNRGGYCNFMHLKRISRELRHELFASYHRRRSHSRSRSRSPYRHRSYEERHYGRHGHSR 240

Query: 241 RYDERDAYHESRSRRHRTTSPGHR-SRSRSPRGRKNQSPVREGSEERRAKIEQWNREREQ 300
           RYDERDAYH+SR+RRHR+TSPGHR SRSRSPRGRKN+SPVREGSEERRAKIEQWNRE+EQ
Sbjct: 241 RYDERDAYHDSRNRRHRSTSPGHRGSRSRSPRGRKNRSPVREGSEERRAKIEQWNREKEQ 300

Query: 301 GNIDNNPNSNDNKNSHENSHNGDVKYANQTCGYEEQRQRQ--PPEQGYGY 348
               NN NS+ ++NSHE S+NGDV+Y NQTCGY++Q+Q+Q  PPEQGYGY
Sbjct: 301 QKNYNNSNSDGHENSHEKSYNGDVQYENQTCGYKQQQQQQQKPPEQGYGY 330

BLAST of ClCG01G012750 vs. ExPASy Swiss-Prot
Match: Q9FMY5 (Splicing factor U2af small subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF35B PE=1 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 2.5e-104
Identity = 209/313 (66.77%), Postives = 233/313 (74.44%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVD QG P+DP KIQDHFE+FYED+F+ELNK+GE+ESLNVCDNLADHM+GNVYV F+EE+
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
            AA AL+ L GRFY+                    GRPII DFSPVTDFREATCRQYEEN
Sbjct: 121 HAAAALQALQGRFYS--------------------GRPIIADFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYR---RRRSHSRSRSRSPYRHRSY-EERSYG-- 240
            CNRGGYCNFMH+K+ISRELR +LF  YR   RR S SRSRS SP R R +  ER  G  
Sbjct: 181 SCNRGGYCNFMHVKQISRELRRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDV 240

Query: 241 ----RHGHSRRYDERDAYHE---SRSRRHRTTSPGHRSRSRSPRGRKNQSPVREGSEERR 300
               RHG+ +R  +R   H+      RRH     G   RSRSPR       VREGSEERR
Sbjct: 241 RDRDRHGNGKRSSDRSERHDRDGGGRRRH-----GSPKRSRSPRN------VREGSEERR 282

BLAST of ClCG01G012750 vs. ExPASy Swiss-Prot
Match: Q9ZQW8 (Splicing factor U2af small subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=U2AF35A PE=2 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 4.8e-103
Identity = 207/318 (65.09%), Postives = 234/318 (73.58%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH KPS+SPTLLLSNMY RPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNKPSVSPTLLLSNMYLRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           G+DAQGNPIDP KIQ  FE+FYED+F+EL+KYGEIESL+VCDN ADHM+GNVYVQFREE+
Sbjct: 61  GIDAQGNPIDPEKIQADFEDFYEDIFEELSKYGEIESLHVCDNFADHMIGNVYVQFREED 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
           QAA AL+ L+GR+Y+                    GRPIIV+FSPV+DFREATCRQYEEN
Sbjct: 121 QAARALQALTGRYYS--------------------GRPIIVEFSPVSDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFA-IYRRRRSHS--RSRSRSPYRHRSYEERSYGRHG 240
            CNRGGYCNFMH+K I R+LR  LF  ++R RRSHS  RSRS SPY +R    R Y R  
Sbjct: 181 SCNRGGYCNFMHVKEIGRDLRKRLFGHLHRSRRSHSHGRSRSPSPYHYR----RDYDRRS 240

Query: 241 HSRRYDERDAYH-----------ESRSRRHRTT--SPGHRSRSRSPRGRKNQSPVREGSE 300
            SR  D  D Y               S RHR++  S G R R RS    + +SPVR+GSE
Sbjct: 241 SSRSRDHDDYYRGGSHDYYRGGSRRSSERHRSSYDSDGSRRRHRS----RTRSPVRDGSE 290

Query: 301 ERRAKIEQWNREREQGNI 303
           ERRA+IEQWNRERE   +
Sbjct: 301 ERRAQIEQWNREREAAQV 290

BLAST of ClCG01G012750 vs. ExPASy Swiss-Prot
Match: Q9S709 (Splicing factor U2af small subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF35A PE=1 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 4.1e-102
Identity = 201/312 (64.42%), Postives = 227/312 (72.76%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQG P+DPRKIQ+HFE+F+EDLF+EL K+GEIESLN+CDNLADHM+GNVYVQF+EE+
Sbjct: 61  GVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIGNVYVQFKEED 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
           QAA AL+ L GRFY+                    GRPII DFSPVTDFREATCRQYEEN
Sbjct: 121 QAAAALQALQGRFYS--------------------GRPIIADFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYRR-----RRSHSRSRSRSPYRHRSYEERSYGR 240
            CNRGGYCNFMH+K +SRELR +LF  YRR      RS SRSRS SP   R  + R    
Sbjct: 181 NCNRGGYCNFMHVKLVSRELRRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSH 240

Query: 241 HGHSRRYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKNQSPV--------REGSEERR 300
              S R  +R+ Y     +  R++    R      RGR+  SP         REGSEERR
Sbjct: 241 REFSHRDRDREFYRHGSGK--RSSERSERQERDGSRGRRQASPKRGGSPGGGREGSEERR 290

BLAST of ClCG01G012750 vs. ExPASy Swiss-Prot
Match: Q6AUG0 (Splicing factor U2af small subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=U2AF35B PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 9.7e-96
Identity = 194/324 (59.88%), Postives = 225/324 (69.44%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P++SPT++L+NMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTVSPTIVLANMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQG PIDP K+Q+HFE+FYED+++EL+K+GE+E+LNVCDNLADHM+GNVYVQFREEE
Sbjct: 61  GVDAQGQPIDPEKMQEHFEDFYEDIYEELSKFGEVETLNVCDNLADHMIGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
           QA  A   L GRFY+                    GRPIIV++SPVTDFREATCRQ+EEN
Sbjct: 121 QAVAAHNALQGRFYS--------------------GRPIIVEYSPVTDFREATCRQFEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYRRRRSHSRSRSRSPYRHRSYEE---------- 240
            CNRGGYCNFMH+K+I RELR +L+   R RRSH RSRS SP   R   +          
Sbjct: 181 SCNRGGYCNFMHVKQIGRELRRKLYG-GRSRRSHGRSRSPSPRHRRGNRDRDDFRRERDG 240

Query: 241 --------RSYGRHGHSRRYDERDAY-------HESRSRRHRTTSPGHRSRSRSPRGRKN 300
                   R  G  G    Y   D+Y             R+     G R R  SP  R+ 
Sbjct: 241 YRGGGDGYRGGGGGGGGDGYRGGDSYRGGGGGGRRGGGSRYDRYDDGGRRRHGSP-PRRA 300

BLAST of ClCG01G012750 vs. ExPASy Swiss-Prot
Match: A1A4K8 (Splicing factor U2AF 35 kDa subunit OS=Bos taurus OX=9913 GN=U2AF1 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.8e-62
Identity = 134/255 (52.55%), Postives = 163/255 (63.92%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAE+LASIFGTEKD+VNC FYFKIGACRHGDRCSRLH KP+ S T+ L N+Y+ P   + 
Sbjct: 1   MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTIALLNIYRNPQNSSQ 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQEL-NKYGEIESLNVCDNLADHMVGNVYVQFREE 120
             D     +   ++Q+H++EF+E++F E+  KYGE+E +NVCDNL DH+VGNVYV+FR E
Sbjct: 61  SADGLRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRRE 120

Query: 121 EQAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEE 180
           E A  A+ +L+ R++ GQ                    PI  + SPVTDFREA CRQYE 
Sbjct: 121 EDAEKAVIDLNNRWFNGQ--------------------PIHAELSPVTDFREACCRQYEM 180

Query: 181 NMCNRGGYCNFMHLKRISRELRHELFAIYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHS 240
             C RGG+CNFMHLK ISRELR EL   Y RRR   RSRSRS  R     +R  G  G  
Sbjct: 181 GECTRGGFCNFMHLKPISRELRREL---YGRRRKKHRSRSRSRERRSRSRDRGRGGGGGG 231

Query: 241 RRYDERDAYHESRSR 255
               ERD    SR R
Sbjct: 241 GGGRERDR-RRSRDR 231

BLAST of ClCG01G012750 vs. ExPASy TrEMBL
Match: A0A0A0LVA4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G145960 PE=4 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 6.2e-170
Identity = 309/347 (89.05%), Postives = 320/347 (92.22%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR IQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
           QAANALRNLSGRFYA                    GRPIIVDFSPVTDFREATCRQYEEN
Sbjct: 121 QAANALRNLSGRFYA--------------------GRPIIVDFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR 240
           MCNRGGYCNFMHLKRI RELRHELFA+YRRR SHSRSRSRSPYRHRSYEERSYG+HGHSR
Sbjct: 181 MCNRGGYCNFMHLKRIGRELRHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSR 240

Query: 241 RYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKNQSPVREGSEERRAKIEQWNREREQG 300
           RYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKN+SPVREGSEERRAKIEQWN+EREQG
Sbjct: 241 RYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQG 300

Query: 301 NIDNNPNSNDNKNSHENSHNGDVKYANQTCGYEEQRQRQPPEQGYGY 348
           N DNN NS+DN+N+HE S++ +VKYANQTCGYEEQ+QRQPPEQGYGY
Sbjct: 301 N-DNNANSDDNRNNHEKSYDSEVKYANQTCGYEEQQQRQPPEQGYGY 326

BLAST of ClCG01G012750 vs. ExPASy TrEMBL
Match: A0A1S3CFU2 (splicing factor U2af small subunit B-like OS=Cucumis melo OX=3656 GN=LOC103500459 PE=4 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.1e-169
Identity = 310/347 (89.34%), Postives = 319/347 (91.93%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
           QAANALRNLSGRFYA                    GRPIIVDFSPVTDFREATCRQYEEN
Sbjct: 121 QAANALRNLSGRFYA--------------------GRPIIVDFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR 240
           MCNRGGYCNFMHLKRI RELRHELFA+YRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR
Sbjct: 181 MCNRGGYCNFMHLKRIGRELRHELFAMYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR 240

Query: 241 RYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKNQSPVREGSEERRAKIEQWNREREQG 300
           RYDERDAYHE+RSRR RTTSPGHRSRSRSPRGRKN+SP REGSEERRAKIEQWNREREQG
Sbjct: 241 RYDERDAYHENRSRRRRTTSPGHRSRSRSPRGRKNRSPAREGSEERRAKIEQWNREREQG 300

Query: 301 NIDNNPNSNDNKNSHENSHNGDVKYANQTCGYEEQRQRQPPEQGYGY 348
           N DNN  S+DN+NSHE S++ +VKYANQTCGYEEQ+QRQPPEQGYGY
Sbjct: 301 N-DNNAKSDDNRNSHEKSYDSEVKYANQTCGYEEQQQRQPPEQGYGY 326

BLAST of ClCG01G012750 vs. ExPASy TrEMBL
Match: A0A5D3CV58 (Splicing factor U2af small subunit B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold259G00620 PE=4 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.1e-169
Identity = 310/347 (89.34%), Postives = 319/347 (91.93%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
           QAANALRNLSGRFYA                    GRPIIVDFSPVTDFREATCRQYEEN
Sbjct: 121 QAANALRNLSGRFYA--------------------GRPIIVDFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR 240
           MCNRGGYCNFMHLKRI RELRHELFA+YRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR
Sbjct: 181 MCNRGGYCNFMHLKRIGRELRHELFAMYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR 240

Query: 241 RYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKNQSPVREGSEERRAKIEQWNREREQG 300
           RYDERDAYHE+RSRR RTTSPGHRSRSRSPRGRKN+SP REGSEERRAKIEQWNREREQG
Sbjct: 241 RYDERDAYHENRSRRRRTTSPGHRSRSRSPRGRKNRSPAREGSEERRAKIEQWNREREQG 300

Query: 301 NIDNNPNSNDNKNSHENSHNGDVKYANQTCGYEEQRQRQPPEQGYGY 348
           N DNN  S+DN+NSHE S++ +VKYANQTCGYEEQ+QRQPPEQGYGY
Sbjct: 301 N-DNNAKSDDNRNSHEKSYDSEVKYANQTCGYEEQQQRQPPEQGYGY 326

BLAST of ClCG01G012750 vs. ExPASy TrEMBL
Match: A0A6J1HM65 (splicing factor U2af small subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC111464850 PE=4 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.2e-162
Identity = 301/350 (86.00%), Postives = 316/350 (90.29%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQF+EEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEE 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
           QAANALRNLSGRFYA                    GRPIIVDFSPVTDFREATCRQYEEN
Sbjct: 121 QAANALRNLSGRFYA--------------------GRPIIVDFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR 240
           MCNRGGYCNFMHLKRISRELRHELFA Y RRRSHSRSRSRSPYRHRSYEER YGRHGHSR
Sbjct: 181 MCNRGGYCNFMHLKRISRELRHELFASYHRRRSHSRSRSRSPYRHRSYEERHYGRHGHSR 240

Query: 241 RYDERDAYHESRSRRHRTTSPGHR-SRSRSPRGRKNQSPVREGSEERRAKIEQWNREREQ 300
           RYDERDAYH+SR+RRHR+TSPGHR SRSRSPRGRKN+SPVREGSEERRAKIEQWNRE+EQ
Sbjct: 241 RYDERDAYHDSRNRRHRSTSPGHRGSRSRSPRGRKNRSPVREGSEERRAKIEQWNREKEQ 300

Query: 301 GNIDNNPNSNDNKNSHENSHNGDVKYANQTCGYEEQRQRQ--PPEQGYGY 348
               NN NS+ ++NSHE S+NGDV+Y NQTCGY++Q+Q+Q  PPEQGYGY
Sbjct: 301 QKNYNNSNSDGHENSHEKSYNGDVQYENQTCGYKQQQQQQQKPPEQGYGY 330

BLAST of ClCG01G012750 vs. ExPASy TrEMBL
Match: A0A6J1I2J7 (splicing factor U2af small subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC111469321 PE=4 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.3e-161
Identity = 300/350 (85.71%), Postives = 315/350 (90.00%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQF+EEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEE 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
           QAANALRNLSGRFYA                    GRPIIVDFSPVTDFREATCRQYEEN
Sbjct: 121 QAANALRNLSGRFYA--------------------GRPIIVDFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSR 240
           MCNRGGYCNFMHLKRISRELRHELFA Y RRR HSRSRSRSPYRHRSYEER YGRHGHSR
Sbjct: 181 MCNRGGYCNFMHLKRISRELRHELFASYHRRRGHSRSRSRSPYRHRSYEERHYGRHGHSR 240

Query: 241 RYDERDAYHESRSRRHRTTSPGHR-SRSRSPRGRKNQSPVREGSEERRAKIEQWNREREQ 300
           RYDERDAYH+SR+RRHR+TSPGHR SRSRSPRGRKN+SPVRE SEERRAKIEQWNRE+EQ
Sbjct: 241 RYDERDAYHDSRNRRHRSTSPGHRGSRSRSPRGRKNRSPVREESEERRAKIEQWNREKEQ 300

Query: 301 GNIDNNPNSNDNKNSHENSHNGDVKYANQTCGY--EEQRQRQPPEQGYGY 348
            N  NN NS+ N++SHE S+NGDV+Y NQTCGY  ++Q+Q+QPPEQGYGY
Sbjct: 301 ENY-NNANSDGNEHSHEKSYNGDVQYENQTCGYKQQQQQQQQPPEQGYGY 329

BLAST of ClCG01G012750 vs. TAIR 10
Match: AT5G42820.2 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 380.2 bits (975), Expect = 1.8e-105
Identity = 209/313 (66.77%), Postives = 233/313 (74.44%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVD QG P+DP KIQDHFE+FYED+F+ELNK+GE+ESLNVCDNLADHM+GNVYV F+EE+
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
            AA AL+ L GRFY+                    GRPII DFSPVTDFREATCRQYEEN
Sbjct: 121 HAAAALQALQGRFYS--------------------GRPIIADFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYR---RRRSHSRSRSRSPYRHRSY-EERSYG-- 240
            CNRGGYCNFMH+K+ISRELR +LF  YR   RR S SRSRS SP R R +  ER  G  
Sbjct: 181 SCNRGGYCNFMHVKQISRELRRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDV 240

Query: 241 ----RHGHSRRYDERDAYHE---SRSRRHRTTSPGHRSRSRSPRGRKNQSPVREGSEERR 300
               RHG+ +R  +R   H+      RRH     G   RSRSPR       VREGSEERR
Sbjct: 241 RDRDRHGNGKRSSDRSERHDRDGGGRRRH-----GSPKRSRSPRN------VREGSEERR 282

BLAST of ClCG01G012750 vs. TAIR 10
Match: AT5G42820.1 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 380.2 bits (975), Expect = 1.8e-105
Identity = 209/313 (66.77%), Postives = 233/313 (74.44%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVD QG P+DP KIQDHFE+FYED+F+ELNK+GE+ESLNVCDNLADHM+GNVYV F+EE+
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
            AA AL+ L GRFY+                    GRPII DFSPVTDFREATCRQYEEN
Sbjct: 121 HAAAALQALQGRFYS--------------------GRPIIADFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYR---RRRSHSRSRSRSPYRHRSY-EERSYG-- 240
            CNRGGYCNFMH+K+ISRELR +LF  YR   RR S SRSRS SP R R +  ER  G  
Sbjct: 181 SCNRGGYCNFMHVKQISRELRRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDV 240

Query: 241 ----RHGHSRRYDERDAYHE---SRSRRHRTTSPGHRSRSRSPRGRKNQSPVREGSEERR 300
               RHG+ +R  +R   H+      RRH     G   RSRSPR       VREGSEERR
Sbjct: 241 RDRDRHGNGKRSSDRSERHDRDGGGRRRH-----GSPKRSRSPRN------VREGSEERR 282

BLAST of ClCG01G012750 vs. TAIR 10
Match: AT1G27650.1 (U2 snRNP auxiliary factor small subunit, putative )

HSP 1 Score: 372.9 bits (956), Expect = 2.9e-103
Identity = 201/312 (64.42%), Postives = 227/312 (72.76%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQG P+DPRKIQ+HFE+F+EDLF+EL K+GEIESLN+CDNLADHM+GNVYVQF+EE+
Sbjct: 61  GVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIGNVYVQFKEED 120

Query: 121 QAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN 180
           QAA AL+ L GRFY+                    GRPII DFSPVTDFREATCRQYEEN
Sbjct: 121 QAAAALQALQGRFYS--------------------GRPIIADFSPVTDFREATCRQYEEN 180

Query: 181 MCNRGGYCNFMHLKRISRELRHELFAIYRR-----RRSHSRSRSRSPYRHRSYEERSYGR 240
            CNRGGYCNFMH+K +SRELR +LF  YRR      RS SRSRS SP   R  + R    
Sbjct: 181 NCNRGGYCNFMHVKLVSRELRRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSH 240

Query: 241 HGHSRRYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKNQSPV--------REGSEERR 300
              S R  +R+ Y     +  R++    R      RGR+  SP         REGSEERR
Sbjct: 241 REFSHRDRDREFYRHGSGK--RSSERSERQERDGSRGRRQASPKRGGSPGGGREGSEERR 290

BLAST of ClCG01G012750 vs. TAIR 10
Match: AT1G27650.2 (U2 snRNP auxiliary factor small subunit, putative )

HSP 1 Score: 269.2 bits (687), Expect = 4.5e-72
Identity = 154/262 (58.78%), Postives = 178/262 (67.94%), Query Frame = 0

Query: 51  MYQRPDMITPGVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVG 110
           MYQRPDMITPGVDAQG P+DPRKIQ+HFE+F+EDLF+EL K+GEIESLN+CDNLADHM+G
Sbjct: 1   MYQRPDMITPGVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIG 60

Query: 111 NVYVQFREEEQAANALRNLSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFR 170
           NVYVQF+EE+QAA AL+ L GRFY+                    GRPII DFSPVTDFR
Sbjct: 61  NVYVQFKEEDQAAAALQALQGRFYS--------------------GRPIIADFSPVTDFR 120

Query: 171 EATCRQYEENMCNRGGYCNFMHLKRISRELRHELFAIYRR-----RRSHSRSRSRSPYRH 230
           EATCRQYEEN CNRGGYCNFMH+K +SRELR +LF  YRR      RS SRSRS SP   
Sbjct: 121 EATCRQYEENNCNRGGYCNFMHVKLVSRELRRKLFGRYRRSYRRGSRSRSRSRSISPRNK 180

Query: 231 RSYEERSYGRHGHSRRYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKNQSPV------ 290
           R  + R       S R  +R+ Y     +  R++    R      RGR+  SP       
Sbjct: 181 RDNDRRDPSHREFSHRDRDREFYRHGSGK--RSSERSERQERDGSRGRRQASPKRGGSPG 240

Query: 291 --REGSEERRAKIEQWNREREQ 300
             REGSEERRA+IEQWNRERE+
Sbjct: 241 GGREGSEERRARIEQWNREREE 240

BLAST of ClCG01G012750 vs. TAIR 10
Match: AT1G10320.1 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 99.8 bits (247), Expect = 4.7e-21
Identity = 92/317 (29.02%), Postives = 143/317 (45.11%), Query Frame = 0

Query: 9   FGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDAQGNP 68
           FGTE+D+ +CPF+ K GACR G RCSR+H  P+ S TLL+ NMY  P  IT   D +G  
Sbjct: 237 FGTEQDKAHCPFHLKTGACRFGQRCSRVHFYPNKSCTLLMKNMYNGPG-ITWEQD-EGLE 296

Query: 69  IDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEEQAANALRN 128
               + +  +EEFYED+  E  KYGE+ +  VC N + H+ GNVYV +R  E A  A ++
Sbjct: 297 YTDEEAELCYEEFYEDVHTEFLKYGELVNFKVCRNGSFHLKGNVYVHYRSLESAILAYQS 356

Query: 129 LSGRFYAGQLEFLSPPFPLSCPLFTDHGRPIIVDFSPVTDFREATCRQYEEN---MCNRG 188
           ++GR++A                    G+ +  +F  ++ ++ A C +Y ++    C+RG
Sbjct: 357 INGRYFA--------------------GKQVNCEFVNISRWKVAICGEYMKSRLKTCSRG 416

Query: 189 GYCNFMHLKRISRELRHELFAIYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSRRYDER 248
             CNF+H               +R                R +  +     G+S   DE+
Sbjct: 417 SACNFIH--------------CFRNPGGDYEWADHDRPPPRFWIHKMTSLFGYS---DEK 476

Query: 249 DAYHESRSRRHRTTSPGHRSRSRSPRGRKNQSPVREGSEERRAKIEQWNREREQGNIDNN 308
              HES    + + S       R P  R   S  R+           +   +  G+  ++
Sbjct: 477 HMEHESSGSLNDSISDLSTDSHRQPSRR---SRSRDHDHANVGSTPSYRSRKYHGDTQDS 511

Query: 309 PNSNDNKNSHENSHNGD 323
              +  +   EN H+GD
Sbjct: 537 TREDKLRRHAENCHDGD 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004139466.11.3e-16989.05splicing factor U2af small subunit B [Cucumis sativus] >XP_011654262.1 splicing ... [more]
XP_008461981.12.2e-16989.34PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >XP_00846198... [more]
XP_038894442.11.6e-16789.63splicing factor U2af small subunit B-like [Benincasa hispida] >XP_038894443.1 sp... [more]
XP_023519661.16.8e-16386.29splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_02351... [more]
XP_022964867.12.6e-16286.00splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022964868.1 s... [more]
Match NameE-valueIdentityDescription
Q9FMY52.5e-10466.77Splicing factor U2af small subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF35B ... [more]
Q9ZQW84.8e-10365.09Splicing factor U2af small subunit A OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q9S7094.1e-10264.42Splicing factor U2af small subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF35A ... [more]
Q6AUG09.7e-9659.88Splicing factor U2af small subunit B OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
A1A4K81.8e-6252.55Splicing factor U2AF 35 kDa subunit OS=Bos taurus OX=9913 GN=U2AF1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LVA46.2e-17089.05Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G145960 PE=4 SV=1[more]
A0A1S3CFU21.1e-16989.34splicing factor U2af small subunit B-like OS=Cucumis melo OX=3656 GN=LOC10350045... [more]
A0A5D3CV581.1e-16989.34Splicing factor U2af small subunit B-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A6J1HM651.2e-16286.00splicing factor U2af small subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1I2J72.3e-16185.71splicing factor U2af small subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT5G42820.21.8e-10566.77Zinc finger C-x8-C-x5-C-x3-H type family protein [more]
AT5G42820.11.8e-10566.77Zinc finger C-x8-C-x5-C-x3-H type family protein [more]
AT1G27650.12.9e-10364.42U2 snRNP auxiliary factor small subunit, putative [more]
AT1G27650.24.5e-7258.78U2 snRNP auxiliary factor small subunit, putative [more]
AT1G10320.14.7e-2129.02Zinc finger C-x8-C-x5-C-x3-H type family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009145U2 auxiliary factor small subunitPRINTSPR01848U2AUXFACTORcoord: 150..174
score: 49.82
coord: 37..57
score: 57.14
coord: 18..37
score: 85.68
coord: 75..90
score: 50.57
coord: 185..197
score: 66.78
IPR009145U2 auxiliary factor small subunitPANTHERPTHR12620U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNITcoord: 1..328
IPR003954RNA recognition motif domain, eukaryoteSMARTSM00361rrm2_1coord: 73..142
e-value: 3.0E-4
score: 30.1
IPR000571Zinc finger, CCCH-typeSMARTSM00356c3hfinal6coord: 13..39
e-value: 0.0065
score: 25.7
coord: 168..194
e-value: 0.21
score: 20.6
IPR000571Zinc finger, CCCH-typePFAMPF00642zf-CCCHcoord: 14..37
e-value: 5.7E-7
score: 29.3
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 168..195
score: 10.774557
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 12..40
score: 13.112443
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 43..140
e-value: 7.7E-27
score: 95.6
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 83..136
e-value: 7.9E-6
score: 25.6
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 44..166
score: 10.462521
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 222..255
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..273
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 274..298
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 299..336
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 211..347
NoneNo IPR availablePANTHERPTHR12620:SF39SPLICING FACTOR U2AF SMALL SUBUNIT Acoord: 1..328
NoneNo IPR availableCDDcd12539RRM_U2AF35Bcoord: 44..165
e-value: 7.95692E-60
score: 186.031
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 77..137

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG01G012750.2ClCG01G012750.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0005681 spliceosomal complex
cellular_component GO:0089701 U2AF complex
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030628 pre-mRNA 3'-splice site binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003723 RNA binding