ClCG01G011450 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG01G011450
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	Subtilisin-like protease SBT5.4
Location	CG_Chr01: 18797760 .. 18801327 (+)
RNA-Seq Expression	ClCG01G011450
Synteny	ClCG01G011450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAAAGATTTTGATTCCAAGTGGTTCAAAGTTTGTAATGGTAAAACCACCTTAGGATACTAAGGAAAGCTCAGGGCAAGAGACAAACCATCGAGGCAAATTTTGTTCTCAGATGAGGTAGGACACGAGTAGAGCCTATCAATGGCGTACCTAGTTAATGTGTCTAAATAATCAAGTGAAATGAAATAAATATAGATAAATATAGAGTGGGATGGAAAAGATAGAAAATCAATAAAACAAAGTTGAGTAGAAAAACTCATTAGATACCATTGCTTCTTTACGATATGGGAAAGGGCCTTCCTTGGTAGCCCACGCTTCGCACCCTTCTAATTCTGGTTGGGATGCTGGATCCTCAGTCCTACCCCTAGCAAGTCAAGTCAGTTCCCATTAATAAAATTAGGAAATTTTCCATAGCCTTGATTTGTGAGTTTAATCTCCAATGTTTCTTGAAGGCAATTCAAGTTTATATATATATTTTTTTTCTTCAGATTGTTAGGTAGTTAGTTATCTTGATGATTTTTTACTTATATGCTTAATAGTTTAAAATTTTGTTCAGTGTTATAAGCATGTATGGACACAAAAAGATATATAAAAAAGGAATCACATGCGAAAGAGTGAGAGAACTAGACAACAAAGTTCATAGTTGAGCTCATCTCATTTTGGCAACTAACCAAAAAAAAATAATTTATTTATATATTTATTAAACTGCAAACTACACAATGTGAAAAAAAAAAAAAAAAAAAAAACTGATAAAAGAAATATATTTTAAAAAACAAAATTGATGAAAGACAAATGTAATTTTTAAACAAAATTGTGAAGTATAAAAATGTCATTTTAAGGATTCTCAAACATCTAAGGATGCTACATATTTTGGTAAAGAATAGTCGAGGTAATTCCAGCCCCTAAATTTAATTCATATAATTCTACAAAAATATTTCAAGCATGTCATAAGTTTCAATTCAACTCATTTCTTATTAGGTTTTATATTACATTATTGAATTTGAATTTGATTTGAATTTTGTTATTTTCCTAATATTTCAAACTTTTTTTAAAAAAAATACATCTACCTTAACTAAGTAGTTTACCTTACTATTCTGTAAATGCCCCTTGAAGAAATATAGGTTTTTGAACTCCTCTCTCCTCCAAAACTAAGATTTTAACAATTCTCTCAAGACTTTGATCAAGATGGACTACACAATTTTAAGTTCGGTTTTTTGTTTTTAGCTTTTGTTATCAAACAGGCCTTACTCATCCAAATTTAATTCCATGTAATTGCTATTTTGATTAGATTAAGCATCATGCTGTTGAACTTAAAAAAAAAAACTATATATATCAACTTCCCAACATTTGTAATTCAATATTCTAAACAAAATATATATTCTTCTAACTCAAATACAATATTCTGAATCTAATCAAGTAAATATCTGGACAACCAAAATAAAAGGAAAAAAAAAAAAATAACAAAGTTCTCTCAGTTACAAAATAAATCAAAAAAGTTTAATAACAATTTGGAAAACCTACAAAAACGCAACGAACACGATCAAAACTAAATCAGCCGAGCCTACCGTGAAAGTATCTGAGAAAAAGATTCATGAGTGAAGTGGTTTGAAACCTACAAAAAGGCTTTAAATTGTTTTAAGAAACTTAATTAATTTAAACTTAACTTAATGATCACTCTTTTACTTGTGAAAATTTAGTCAAATTTTTATATCGGTTTAAAAATAAATAAATTGAAAGATTTAGTTCAGAATCTTCTCTTGAAAAAGAAAAAAAGAAACTTGAATATACAAAAGTATAGATTCCATATAAATCAAAGAAAAATATTATCACGAGACCTTACTTCTTCAAATTTGAATATATTTCTACAATTAAAATAATAATAATTTTCTAAATAATAAAATAAAAGGCAAAAAAAGGTTATCTCAAATAATAAGATCTTATCCCAATTCCACAGGGAATATAATAAGCATGTTATGATAATTAAATTAATTCTATTGCCCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTATTTAGCAAAGGCTTTCCTTTATAAAGAGGAGGGAGCTTTGAAGAAAGTGATTAATTGTTAGGTTTTTGGAGATCATCAAAAAGCATCCACAGATGAAGATATCTAATTCCATCTTTATTTTATTTTCATTATTCCTTTTGTTAATATCACCTGCCATTGCTGCTAAAAAGGTAACCAAAACAACAGGCTTTTGTTTGGGAATTTGTTTCGCTTTCAGCCACCAAAAAGTTAGAAAAGAGATCCATTCTTAAACAAGTTTTAAGTTGTATTATCATGCATGCATTATCTAAAATCTATACCCATTTTGATCTTTTTTATTTTTTTTCTTTTTGTTAATTGATTTGGTTTGATCATAAAAGGTGTTTTTGGGTTGTTGTTTTCTTCTCTAATAATTGTTTTTTTCTTTTGGTACTCCTTGATTGATTCAGTGGTCTACTTGTAATGTCTCTATTCTAAGCATATTATTAAACAAACCCTAAAACTATATTTTTTTTCTTTTATTGATTTGGTTTGGTCATATGAGGTATTTTTGGTTTGTGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAAAACCCTAAAATTTTTTTGTTTTCTTCTTCTACTTTTTCTTTCTTGTAATCCTTCATTGATTCAAAGGTGTACTCGGTATGTTTCTAAGCATATTATTGAACAAACCCTAAAAACTAATTGCTTTTTTTTTTCTTTTTTGTTGGGATTTCAGTCATATGTTGTGATATTGGGATCCCATTCACATGGGTTAGAAGTTACTGAGGAAGATCTTGAAAGGGTGGTTCATTCCCATCACAAATTGCTTGGATCCTTCTTGGGCAGGTTTGAAACTTTTATCTAATTAATTTCCTCATCTTAACAATTTTGTTTCTTCCTTTTAAATTAATCAATTATTATTAGTTTTAATATTTTAAAAATGAAAATTTCAGTGATGAGAAGGCAAAAGAGGCCATATTTTACTCCTATAAGAAGCATATTAACGGCTTTGCAGCCATTCTGGACGAGGAAGACGCAACCCAACTTGCAAGTAAAAACCAAACTGTATATATTTTAGCATAACGAATGTTGGTTAATTAAAATAGAATTATAATAATGGAAAATTATAAAAATGGGACAGATCATCCCGAGGTAGCAGCAGTATTGCCAAACAGAGCGAAAGAATTACACACAACACATTCATGGGAATTTATGCATTTGGAGAAGAATGGGGTGATTCCTCCTTCTTCTCCTTGGCACAGGGCTAAATCTGGAAAAGATGTCATTATTGCCAACCTTGACACTGGTATTTTTTTTTTTTTTAATTTTCTTTTTAATTGTTTGAGAAATTGGTCTTTTCATATGCACGTATGTATGCACATTCTGAAATTGATAATTTTAGAGCTAAATTTATATGGCCAAAACTTTATTTTATTAATTTATTAATTTATTTTTTTACAAATTTAGTGGTGAATAGTGAGGAATTCAACGAACATAAAAACATAGAATAA

mRNA sequence

ATGGTGAAAGATTTTGATTCCAAGTGGTTCAAAGTTTGTAATGATACCATTGCTTCTTTACGATATGGGAAAGGGCCTTCCTTGGTAGCCCACGCTTCGCACCCTTCTAATTCTGGTTGGGATGCTGGATCCTCAGTCCTACCCCTAGCAAGTCAATCATATGTTGTGATATTGGGATCCCATTCACATGGGTTAGAAGTTACTGAGGAAGATCTTGAAAGGGTGGTTCATTCCCATCACAAATTGCTTGGATCCTTCTTGGGCAGTGATGAGAAGGCAAAAGAGGCCATATTTTACTCCTATAAGAAGCATATTAACGGCTTTGCAGCCATTCTGGACGAGGAAGACGCAACCCAACTTGCAAATCATCCCGAGGTAGCAGCAGTATTGCCAAACAGAGCGAAAGAATTACACACAACACATTCATGGGAATTTATGCATTTGGAGAAGAATGGGGTGATTCCTCCTTCTTCTCCTTGGCACAGGGCTAAATCTGGAAAAGATGTCATTATTGCCAACCTTGACACTGTGGTGAATAGTGAGGAATTCAACGAACATAAAAACATAGAATAA

Coding sequence (CDS)

ATGGTGAAAGATTTTGATTCCAAGTGGTTCAAAGTTTGTAATGATACCATTGCTTCTTTACGATATGGGAAAGGGCCTTCCTTGGTAGCCCACGCTTCGCACCCTTCTAATTCTGGTTGGGATGCTGGATCCTCAGTCCTACCCCTAGCAAGTCAATCATATGTTGTGATATTGGGATCCCATTCACATGGGTTAGAAGTTACTGAGGAAGATCTTGAAAGGGTGGTTCATTCCCATCACAAATTGCTTGGATCCTTCTTGGGCAGTGATGAGAAGGCAAAAGAGGCCATATTTTACTCCTATAAGAAGCATATTAACGGCTTTGCAGCCATTCTGGACGAGGAAGACGCAACCCAACTTGCAAATCATCCCGAGGTAGCAGCAGTATTGCCAAACAGAGCGAAAGAATTACACACAACACATTCATGGGAATTTATGCATTTGGAGAAGAATGGGGTGATTCCTCCTTCTTCTCCTTGGCACAGGGCTAAATCTGGAAAAGATGTCATTATTGCCAACCTTGACACTGTGGTGAATAGTGAGGAATTCAACGAACATAAAAACATAGAATAA

Protein sequence

MVKDFDSKWFKVCNDTIASLRYGKGPSLVAHASHPSNSGWDAGSSVLPLASQSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAAILDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDVIIANLDTVVNSEEFNEHKNIE

Homology

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BLAST of ClCG01G011450 vs. NCBI nr
Match: XP_038895602.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 235.7 bits (600), Expect = 3.2e-58
Identity = 115/139 (82.73%), Postives = 125/139 (89.93%), Query Frame = 0


Query: 50  ASQSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFA 109
           A +SYVV+LGSHSHG+E+TEEDLERVVHSHHKLLGSFLGS+EKAK+AIFYSYKKHINGFA
Sbjct: 23  AKKSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHINGFA 82

Query: 110 AILDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDV 169
           A LDEE+AT+LA HPEVAA+L NRAK LHTTHSWEFMHLEKNGVIP SSPW  AKSGKDV
Sbjct: 83  ATLDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPSSSPWRIAKSGKDV 142

Query: 170 IIANLDTVV--NSEEFNEH 187
           IIANLDT V   S+ F EH
Sbjct: 143 IIANLDTGVWPESKSFGEH 161

[Close]

BLAST of ClCG01G011450 vs. NCBI nr
Match: XP_031739111.1 (subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 231.9 bits (590), Expect = 4.7e-57
Identity = 114/142 (80.28%), Postives = 124/142 (87.32%), Query Frame = 0


Query: 47  LPLASQSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHIN 106
           L L  QSY+V+LGSHSHGLEVT+EDL+RV  SHHKLLGS  GSDEKA+ AIFYSYKK+IN
Sbjct: 6   LMLGFQSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNIN 65

Query: 107 GFAAILDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSG 166
           GFAAI+DEE+A QLA HPEVAAVLPNRAK+LHTTHSWEFMHLEKNGVIPPSS W RAKSG
Sbjct: 66  GFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSG 125

Query: 167 KDVIIANLDTVV--NSEEFNEH 187
           KDVIIANLDT V   S+ F EH
Sbjct: 126 KDVIIANLDTGVWPESKSFGEH 147

[Close]

BLAST of ClCG01G011450 vs. NCBI nr
Match: KAE8652826.1 (hypothetical protein Csa_022873 [Cucumis sativus])

HSP 1 Score: 228.4 bits (581), Expect = 5.2e-56
Identity = 111/136 (81.62%), Postives = 121/136 (88.97%), Query Frame = 0


Query: 53  SYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAAIL 112
           SY+V+LGSHSHGLEVT+EDL+RV  SHHKLLGS  GSDEKA+ AIFYSYKK+INGFAAI+
Sbjct: 8   SYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNINGFAAIM 67

Query: 113 DEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDVIIA 172
           DEE+A QLA HPEVAAVLPNRAK+LHTTHSWEFMHLEKNGVIPPSS W RAKSGKDVIIA
Sbjct: 68  DEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIA 127

Query: 173 NLDTVV--NSEEFNEH 187
           NLDT V   S+ F EH
Sbjct: 128 NLDTGVWPESKSFGEH 143

[Close]

BLAST of ClCG01G011450 vs. NCBI nr
Match: TYK15479.1 (subtilisin-like protease SBT5.4 [Cucumis melo var. makuwa])

HSP 1 Score: 226.9 bits (577), Expect = 1.5e-55
Identity = 110/136 (80.88%), Postives = 121/136 (88.97%), Query Frame = 0


Query: 53  SYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAAIL 112
           SYVV+LGSHSHGLEVTE+D E VV SHHKLLGSFL SDEKAK++IFYSYKKHINGFAA L
Sbjct: 755 SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL 814

Query: 113 DEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDVIIA 172
           D+EDAT+LANHPEVAAVLPN+ K+L+TTHSWEFMHLEKNGV+PPSSPW  AK GKDVIIA
Sbjct: 815 DDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVIIA 874

Query: 173 NLDTVV--NSEEFNEH 187
           NLDT V   S+ F EH
Sbjct: 875 NLDTGVWPESKSFGEH 890

[Close]

BLAST of ClCG01G011450 vs. NCBI nr
Match: XP_011648463.1 (subtilisin-like protease SBT5.4 [Cucumis sativus] >KGN64981.1 hypothetical protein Csa_022745 [Cucumis sativus])

HSP 1 Score: 225.3 bits (573), Expect = 4.4e-55
Identity = 110/137 (80.29%), Postives = 123/137 (89.78%), Query Frame = 0


Query: 52  QSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAAI 111
           +SYVV+LGSHSHGL+ TE+D +RVV SHHKLLGSFL S+EKAK+AIFYSYKK+INGFAA 
Sbjct: 25  KSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLRSEEKAKDAIFYSYKKNINGFAAT 84

Query: 112 LDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDVII 171
           LD+EDAT+LANHPEVAAVLPN+AK L+TTHSWEFMHLEKNGVIPPSSPW RAK GKDVII
Sbjct: 85  LDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHLEKNGVIPPSSPWWRAKFGKDVII 144

Query: 172 ANLDTVV--NSEEFNEH 187
           ANLDT V   S+ F EH
Sbjct: 145 ANLDTGVWPESKSFGEH 161

[Close]

BLAST of ClCG01G011450 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-39
Identity = 78/136 (57.35%), Postives = 102/136 (75.00%), Query Frame = 0


Query: 52  QSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAAI 111
           +SY+V LGSH+H  +++   L+ V HSH   L SF+GS E AKEAIFYSYK+HINGFAAI
Sbjct: 40  KSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAI 99

Query: 112 LDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDVII 171
           LDE +A ++A HP+V +V PN+ ++LHTTHSW FM L KNGV+  SS W++A  G+D II
Sbjct: 100 LDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTII 159

Query: 172 ANLDTVV--NSEEFNE 186
           ANLDT V   S+ F++
Sbjct: 160 ANLDTGVWPESKSFSD 175

[Close]

BLAST of ClCG01G011450 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 5.6e-37
Identity = 73/128 (57.03%), Postives = 95/128 (74.22%), Query Frame = 0


Query: 51  SQSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAA 110
           S SYVV  G+HSH  E+TE+ ++RV  +H+  LGSF GS E+A +AIFYSY KHINGFAA
Sbjct: 29  SSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAA 88

Query: 111 ILDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDVI 170
            LD + A +++ HPEV +V PN+A +LHTT SW+F+ LE N  +P SS W +A+ G+D I
Sbjct: 89  HLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTI 148

Query: 171 IANLDTVV 179
           IANLDT V
Sbjct: 149 IANLDTGV 156

[Close]

BLAST of ClCG01G011450 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 133.7 bits (335), Expect = 2.3e-30
Identity = 65/138 (47.10%), Postives = 92/138 (66.67%), Query Frame = 0


Query: 50  ASQSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFA 109
           + + Y+V +G+HSHG   T  DLE    SH+ LLGS  GS EKAKEAI YSY +HINGFA
Sbjct: 28  SKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAIIYSYNRHINGFA 87

Query: 110 AILDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDV 169
           A+L+EE+A  +A +P V +V  ++  +LHTT SWEF+ L + G    +S W + + G++ 
Sbjct: 88  ALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG---QNSAWQKGRFGENT 147

Query: 170 IIANLDTVV--NSEEFNE 186
           II N+DT V   S+ F++
Sbjct: 148 IIGNIDTGVWPESQSFSD 162

[Close]

BLAST of ClCG01G011450 vs. ExPASy Swiss-Prot
Match: Q9ZSB1 (Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3 SV=2)

HSP 1 Score: 87.8 bits (216), Expect = 1.4e-16
Identity = 55/139 (39.57%), Postives = 78/139 (56.12%), Query Frame = 0


Query: 51  SQSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAA 110
           S+ YVV LG   H      ++ E V  SHH++L S LGS E   ++I YSY+   +GFAA
Sbjct: 27  SKVYVVYLGEKEH------DNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAA 86

Query: 111 ILDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSP--WHRAKSGKD 170
            L E  A Q++  PEV  V+PN   E+ TT +W+++     GV P +S     +A  G +
Sbjct: 87  KLTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYL-----GVSPGNSDSLLQKANMGYN 146

Query: 171 VIIANLDTVV--NSEEFNE 186
           VI+  +DT V   SE FN+
Sbjct: 147 VIVGVIDTGVWPESEMFND 154

[Close]

BLAST of ClCG01G011450 vs. ExPASy Swiss-Prot
Match: Q1PDX5 (Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 3.2e-16
Identity = 51/134 (38.06%), Postives = 75/134 (55.97%), Query Frame = 0


Query: 54  YVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAAILD 113
           ++V LG   H       D E V  SH ++L S LGS + A E+I +SY+   +GFAA L 
Sbjct: 38  HIVYLGEKEH------NDPELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLT 97

Query: 114 EEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDVIIAN 173
           +  A Q++ HP+V  V PN   EL TT +++++ L  +    P    H AK G+D+II  
Sbjct: 98  DSQAEQISEHPDVVQVTPNTFYELQTTRTFDYLGLSHS---TPKGLLHEAKMGEDIIIGV 157

Query: 174 LDTVV--NSEEFNE 186
           LD+ V   S+ FN+
Sbjct: 158 LDSGVWPESQSFND 162

[Close]

BLAST of ClCG01G011450 vs. ExPASy TrEMBL
Match: A0A0A0LVY8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 2.3e-57
Identity = 114/142 (80.28%), Postives = 124/142 (87.32%), Query Frame = 0


Query: 47  LPLASQSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHIN 106
           L L  QSY+V+LGSHSHGLEVT+EDL+RV  SHHKLLGS  GSDEKA+ AIFYSYKK+IN
Sbjct: 6   LMLGFQSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNIN 65

Query: 107 GFAAILDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSG 166
           GFAAI+DEE+A QLA HPEVAAVLPNRAK+LHTTHSWEFMHLEKNGVIPPSS W RAKSG
Sbjct: 66  GFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSG 125

Query: 167 KDVIIANLDTVV--NSEEFNEH 187
           KDVIIANLDT V   S+ F EH
Sbjct: 126 KDVIIANLDTGVWPESKSFGEH 147

[Close]

BLAST of ClCG01G011450 vs. ExPASy TrEMBL
Match: A0A5D3CZ66 (Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00570 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 7.3e-56
Identity = 110/136 (80.88%), Postives = 121/136 (88.97%), Query Frame = 0


Query: 53  SYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAAIL 112
           SYVV+LGSHSHGLEVTE+D E VV SHHKLLGSFL SDEKAK++IFYSYKKHINGFAA L
Sbjct: 755 SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL 814

Query: 113 DEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDVIIA 172
           D+EDAT+LANHPEVAAVLPN+ K+L+TTHSWEFMHLEKNGV+PPSSPW  AK GKDVIIA
Sbjct: 815 DDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVIIA 874

Query: 173 NLDTVV--NSEEFNEH 187
           NLDT V   S+ F EH
Sbjct: 875 NLDTGVWPESKSFGEH 890

[Close]

BLAST of ClCG01G011450 vs. ExPASy TrEMBL
Match: A0A0A0LYF1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171030 PE=3 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 2.1e-55
Identity = 110/137 (80.29%), Postives = 123/137 (89.78%), Query Frame = 0


Query: 52  QSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAAI 111
           +SYVV+LGSHSHGL+ TE+D +RVV SHHKLLGSFL S+EKAK+AIFYSYKK+INGFAA 
Sbjct: 25  KSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLRSEEKAKDAIFYSYKKNINGFAAT 84

Query: 112 LDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDVII 171
           LD+EDAT+LANHPEVAAVLPN+AK L+TTHSWEFMHLEKNGVIPPSSPW RAK GKDVII
Sbjct: 85  LDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHLEKNGVIPPSSPWWRAKFGKDVII 144

Query: 172 ANLDTVV--NSEEFNEH 187
           ANLDT V   S+ F EH
Sbjct: 145 ANLDTGVWPESKSFGEH 161

[Close]

BLAST of ClCG01G011450 vs. ExPASy TrEMBL
Match: A0A1S3CLB7 (subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 2.8e-55
Identity = 109/139 (78.42%), Postives = 123/139 (88.49%), Query Frame = 0


Query: 50  ASQSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFA 109
           A +SY+V+LGSHSHGLEV +EDLERV  SHHKLLGS +GSDEKA++AIFYSYK++INGFA
Sbjct: 28  AKKSYIVLLGSHSHGLEVRDEDLERVADSHHKLLGSIIGSDEKARDAIFYSYKRNINGFA 87

Query: 110 AILDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDV 169
           AI+DEE+ATQLA HPEVAAVL N+AK+LHTTHSWEFMHLEKNGVIPPSS W RAKSGKDV
Sbjct: 88  AIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDV 147

Query: 170 IIANLDTVV--NSEEFNEH 187
           II NLDT V   S+ F EH
Sbjct: 148 IIGNLDTGVWGESKSFGEH 166

[Close]

BLAST of ClCG01G011450 vs. ExPASy TrEMBL
Match: A0A6J1EGQ8 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434031 PE=3 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 2.3e-54
Identity = 106/137 (77.37%), Postives = 122/137 (89.05%), Query Frame = 0


Query: 52  QSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAAI 111
           +SY+V+LGSH+HGLEV+  DL+RVV SHHKLLGSFLGS EKAK+AIFYSYK HINGFAAI
Sbjct: 3   ESYIVLLGSHAHGLEVSAVDLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAI 62

Query: 112 LDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDVII 171
           LDEE+AT+LA HPE+AAVLPN+AKELHTT SWEFMHLEKNGV PPSSPW +A+SG++VII
Sbjct: 63  LDEEEATKLAKHPEIAAVLPNKAKELHTTRSWEFMHLEKNGVSPPSSPWRKARSGQNVII 122

Query: 172 ANLDTVV--NSEEFNEH 187
           ANLDT V   S+ F EH
Sbjct: 123 ANLDTGVWPESKSFGEH 139

[Close]

BLAST of ClCG01G011450 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 164.5 bits (415), Expect = 8.5e-41
Identity = 78/136 (57.35%), Postives = 102/136 (75.00%), Query Frame = 0


Query: 52  QSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAAI 111
           +SY+V LGSH+H  +++   L+ V HSH   L SF+GS E AKEAIFYSYK+HINGFAAI
Sbjct: 40  KSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAI 99

Query: 112 LDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDVII 171
           LDE +A ++A HP+V +V PN+ ++LHTTHSW FM L KNGV+  SS W++A  G+D II
Sbjct: 100 LDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTII 159

Query: 172 ANLDTVV--NSEEFNE 186
           ANLDT V   S+ F++
Sbjct: 160 ANLDTGVWPESKSFSD 175

[Close]

BLAST of ClCG01G011450 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 155.6 bits (392), Expect = 4.0e-38
Identity = 73/128 (57.03%), Postives = 95/128 (74.22%), Query Frame = 0


Query: 51  SQSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAA 110
           S SYVV  G+HSH  E+TE+ ++RV  +H+  LGSF GS E+A +AIFYSY KHINGFAA
Sbjct: 29  SSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAA 88

Query: 111 ILDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDVI 170
            LD + A +++ HPEV +V PN+A +LHTT SW+F+ LE N  +P SS W +A+ G+D I
Sbjct: 89  HLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTI 148

Query: 171 IANLDTVV 179
           IANLDT V
Sbjct: 149 IANLDTGV 156

[Close]

BLAST of ClCG01G011450 vs. TAIR 10
Match: AT4G10530.1 (Subtilase family protein )

HSP 1 Score: 87.8 bits (216), Expect = 1.0e-17
Identity = 55/139 (39.57%), Postives = 78/139 (56.12%), Query Frame = 0


Query: 51  SQSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAA 110
           S+ YVV LG   H      ++ E V  SHH++L S LGS E   ++I YSY+   +GFAA
Sbjct: 27  SKVYVVYLGEKEH------DNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAA 86

Query: 111 ILDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSP--WHRAKSGKD 170
            L E  A Q++  PEV  V+PN   E+ TT +W+++     GV P +S     +A  G +
Sbjct: 87  KLTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYL-----GVSPGNSDSLLQKANMGYN 146

Query: 171 VIIANLDTVV--NSEEFNE 186
           VI+  +DT V   SE FN+
Sbjct: 147 VIVGVIDTGVWPESEMFND 154

[Close]

BLAST of ClCG01G011450 vs. TAIR 10
Match: AT5G11940.1 (Subtilase family protein )

HSP 1 Score: 86.7 bits (213), Expect = 2.3e-17
Identity = 51/134 (38.06%), Postives = 75/134 (55.97%), Query Frame = 0


Query: 54  YVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAAILD 113
           ++V LG   H       D E V  SH ++L S LGS + A E+I +SY+   +GFAA L 
Sbjct: 38  HIVYLGEKEH------NDPELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLT 97

Query: 114 EEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSPWHRAKSGKDVIIAN 173
           +  A Q++ HP+V  V PN   EL TT +++++ L  +    P    H AK G+D+II  
Sbjct: 98  DSQAEQISEHPDVVQVTPNTFYELQTTRTFDYLGLSHS---TPKGLLHEAKMGEDIIIGV 157

Query: 174 LDTVV--NSEEFNE 186
           LD+ V   S+ FN+
Sbjct: 158 LDSGVWPESQSFND 162

[Close]

BLAST of ClCG01G011450 vs. TAIR 10
Match: AT4G10520.1 (Subtilase family protein )

HSP 1 Score: 86.3 bits (212), Expect = 2.9e-17
Identity = 54/139 (38.85%), Postives = 78/139 (56.12%), Query Frame = 0


Query: 51  SQSYVVILGSHSHGLEVTEEDLERVVHSHHKLLGSFLGSDEKAKEAIFYSYKKHINGFAA 110
           S+ YVV LG   H      ++ E V  SHH++L S LGS E   ++I YSY+   +GFAA
Sbjct: 27  SKVYVVYLGEKEH------DNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAA 86

Query: 111 ILDEEDATQLANHPEVAAVLPNRAKELHTTHSWEFMHLEKNGVIPPSSP--WHRAKSGKD 170
            L E  A Q++  PEV  V+PN   E+ TT +W+++     GV P +S     +A  G +
Sbjct: 87  KLTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYL-----GVSPGNSDSLLQKANMGYN 146

Query: 171 VIIANLDTVV--NSEEFNE 186
           VI+  +D+ V   SE FN+
Sbjct: 147 VIVGVIDSGVWPESEMFND 154

The following BLAST results are available for this feature:

BLAST of ClCG01G011450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895602.1	3.2e-58	82.73	subtilisin-like protease SBT5.4 [Benincasa hispida]	[more]
XP_031739111.1	4.7e-57	80.28	subtilisin-like protease SBT5.4 [Cucumis sativus]	[more]
KAE8652826.1	5.2e-56	81.62	hypothetical protein Csa_022873 [Cucumis sativus]	[more]
TYK15479.1	1.5e-55	80.88	subtilisin-like protease SBT5.4 [Cucumis melo var. makuwa]	[more]
XP_011648463.1	4.4e-55	80.29	subtilisin-like protease SBT5.4 [Cucumis sativus] >KGN64981.1 hypothetical prote...	[more]

BLAST of ClCG01G011450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JXC5	1.2e-39	57.35	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]
Q9ZSP5	5.6e-37	57.03	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
I1N462	2.3e-30	47.10	Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P...	[more]
Q9ZSB1	1.4e-16	39.57	Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3...	[more]
Q1PDX5	3.2e-16	38.06	Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2...	[more]

BLAST of ClCG01G011450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVY8	2.3e-57	80.28	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1	[more]
A0A5D3CZ66	7.3e-56	80.88	Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A0A0LYF1	2.1e-55	80.29	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171030 PE=3 SV=1	[more]
A0A1S3CLB7	2.8e-55	78.42	subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=...	[more]
A0A6J1EGQ8	2.3e-54	77.37	subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434031 PE...	[more]

BLAST of ClCG01G011450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59810.1	8.5e-41	57.35	Subtilase family protein	[more]
AT2G04160.1	4.0e-38	57.03	Subtilisin-like serine endopeptidase family protein	[more]
AT4G10530.1	1.0e-17	39.57	Subtilase family protein	[more]
AT5G11940.1	2.3e-17	38.06	Subtilase family protein	[more]
AT4G10520.1	2.9e-17	38.85	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 48..138 e-value: 3.4E-23 score: 83.9
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 53..137 e-value: 7.8E-15 score: 55.3
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 51..181
None	No IPR available	PANTHER	PTHR43806:SF40	BNAC02G33520D PROTEIN	coord: 51..181
None	No IPR available	SUPERFAMILY	54897	Protease propeptides/inhibitors	coord: 54..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG01G011450.1	ClCG01G011450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008270	zinc ion binding