ClCG01G002500 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG01G002500
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	E3 ubiquitin-protein ligase RHA1B-like
Location	CG_Chr01: 2467004 .. 2467537 (+)
RNA-Seq Expression	ClCG01G002500
Synteny	ClCG01G002500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCTTCCCTGCAAGCTACACTGAGCTTCTTCTCCCTAAGCTCGTCATCCACGTATTCTCCTTCCTCGGCTTCCTCCGGAAGCTTATCTCCCTCCTCTTCCGCCTCCTTGGCCTCCAAGATTTCCTCGAACCCGACATTCCTTGGCCCAATCACCCCGATTCCTTCCCCACCGGACCCTTCGCCGACCTCGATCCTCTCTCCGCTGCCGTTCTCCGTGAGATTCTCCCCGTCATCAAGTTTTCCGAACTTCTGGATCCGCCGGAGAGCTGCGCTGTGTGCCTCTACGAGTTCGAGGCCGAAGATGAGATCCGCCGCCTTGCTAACTGCCGTCACATCTTCCACCGTGGATGCCTCGATCGGTGGATCGGTTACGGTCAGAGAACTTGCCCGCTCTGCCGGACGGTGTTTATTCCGCCCGATTTGCGGAGTGGTGGTTGTAATTTTGACGATAGGCTTTGGGAGGATTCGGAAATCTTTGAAGCTCTCCTCAGTGATTCTTCATCTTCAAGTCTTCTCGCTGCTGGTTTGTAG

mRNA sequence

ATGGGCTTCCCTGCAAGCTACACTGAGCTTCTTCTCCCTAAGCTCGTCATCCACGTATTCTCCTTCCTCGGCTTCCTCCGGAAGCTTATCTCCCTCCTCTTCCGCCTCCTTGGCCTCCAAGATTTCCTCGAACCCGACATTCCTTGGCCCAATCACCCCGATTCCTTCCCCACCGGACCCTTCGCCGACCTCGATCCTCTCTCCGCTGCCGTTCTCCGTGAGATTCTCCCCGTCATCAAGTTTTCCGAACTTCTGGATCCGCCGGAGAGCTGCGCTGTGTGCCTCTACGAGTTCGAGGCCGAAGATGAGATCCGCCGCCTTGCTAACTGCCGTCACATCTTCCACCGTGGATGCCTCGATCGGTGGATCGGTTACGGTCAGAGAACTTGCCCGCTCTGCCGGACGGTGTTTATTCCGCCCGATTTGCGGAGTGGTGGTTGTAATTTTGACGATAGGCTTTGGGAGGATTCGGAAATCTTTGAAGCTCTCCTCAGTGATTCTTCATCTTCAAGTCTTCTCGCTGCTGGTTTGTAG

Coding sequence (CDS)

ATGGGCTTCCCTGCAAGCTACACTGAGCTTCTTCTCCCTAAGCTCGTCATCCACGTATTCTCCTTCCTCGGCTTCCTCCGGAAGCTTATCTCCCTCCTCTTCCGCCTCCTTGGCCTCCAAGATTTCCTCGAACCCGACATTCCTTGGCCCAATCACCCCGATTCCTTCCCCACCGGACCCTTCGCCGACCTCGATCCTCTCTCCGCTGCCGTTCTCCGTGAGATTCTCCCCGTCATCAAGTTTTCCGAACTTCTGGATCCGCCGGAGAGCTGCGCTGTGTGCCTCTACGAGTTCGAGGCCGAAGATGAGATCCGCCGCCTTGCTAACTGCCGTCACATCTTCCACCGTGGATGCCTCGATCGGTGGATCGGTTACGGTCAGAGAACTTGCCCGCTCTGCCGGACGGTGTTTATTCCGCCCGATTTGCGGAGTGGTGGTTGTAATTTTGACGATAGGCTTTGGGAGGATTCGGAAATCTTTGAAGCTCTCCTCAGTGATTCTTCATCTTCAAGTCTTCTCGCTGCTGGTTTGTAG

Protein sequence

MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGPFADLDPLSAAVLREILPVIKFSELLDPPESCAVCLYEFEAEDEIRRLANCRHIFHRGCLDRWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALLSDSSSSSLLAAGL

Homology

[Close]

BLAST of ClCG01G002500 vs. NCBI nr
Match: XP_038906771.1 (E3 ubiquitin-protein ligase RHA1B [Benincasa hispida])

HSP 1 Score: 351.7 bits (901), Expect = 3.8e-93
Identity = 169/180 (93.89%), Postives = 173/180 (96.11%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MGFPASYTELLLPKL IHV SFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60

Query: 61  FADLDPLSAAVLREILPVIKFSELLDPPESCAVCLYEFEAEDEIRRLANCRHIFHRGCLD 120
           F DLDPLSAAVLREILPV+KFSELLDPPESCAVCLYEFEA+DEIRRLANCRHIFHRGCLD
Sbjct: 61  FTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALLSD---SSSSSLLAAGL 178
           RWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEAL +D   SSSSSLL+AGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALHTDSSSSSSSSLLSAGL 180

[Close]

BLAST of ClCG01G002500 vs. NCBI nr
Match: KAG6579522.1 (Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016982.1 E3 ubiquitin-protein ligase RHA1B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 334.7 bits (857), Expect = 4.8e-88
Identity = 161/177 (90.96%), Postives = 166/177 (93.79%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MGFPASYTELLLPKL IHV S LGFLRKLISLLFRLLGLQDFLEPDIPWPN  DSF TGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSVLGFLRKLISLLFRLLGLQDFLEPDIPWPNQSDSFTTGP 60

Query: 61  FADLDPLSAAVLREILPVIKFSELLDPPESCAVCLYEFEAEDEIRRLANCRHIFHRGCLD 120
             DLDPLSAAVLREILPV+KFSELLDPPESCAVCLYEFE++DEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALLSDSSSSSLLAAGL 178
           RWIGYGQRTCPLCRTVFIP DLRSG CNFDDRLWEDSEIFEAL SDSSSS+LL+AGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPGDLRSGVCNFDDRLWEDSEIFEALHSDSSSSTLLSAGL 177

[Close]

BLAST of ClCG01G002500 vs. NCBI nr
Match: XP_022995549.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita maxima] >XP_023533861.1 E3 ubiquitin-protein ligase RHA1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 333.6 bits (854), Expect = 1.1e-87
Identity = 160/180 (88.89%), Postives = 167/180 (92.78%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MGFPASYTELLLPKL IHV S LGFLRKLISLLFRLLGLQDFLEPDIPW NH DSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFRLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  FADLDPLSAAVLREILPVIKFSELLDPPESCAVCLYEFEAEDEIRRLANCRHIFHRGCLD 120
             DLDPLSAAVLREILPV++FSELLDPPESCAVCLYEFE +DEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALLSDSSSSS---LLAAGL 178
           RWIGYGQRTCPLCRTVFIPPDLR+GGCNFDDR WEDSE+FEAL +DSSSSS   LL+AGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTPTLLSAGL 180

[Close]

BLAST of ClCG01G002500 vs. NCBI nr
Match: XP_022970110.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita maxima])

HSP 1 Score: 333.2 bits (853), Expect = 1.4e-87
Identity = 160/177 (90.40%), Postives = 165/177 (93.22%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MGFPASYTELLLPKL IHV S LGFLRKLISLLFRLLGLQDFLEPDIPWPN  DSF TGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSILGFLRKLISLLFRLLGLQDFLEPDIPWPNQSDSFTTGP 60

Query: 61  FADLDPLSAAVLREILPVIKFSELLDPPESCAVCLYEFEAEDEIRRLANCRHIFHRGCLD 120
             DLDPLSAAVLREILPV+KFSELLDPPESCAVCLYEFE++DEIR LANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFESDDEIRHLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALLSDSSSSSLLAAGL 178
           RWIGYGQRTCPLCRTVFIP DLRSG CNFDDRLWEDSEIFEAL SDSSSS+LL+AGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPGDLRSGVCNFDDRLWEDSEIFEALHSDSSSSTLLSAGL 177

[Close]

BLAST of ClCG01G002500 vs. NCBI nr
Match: XP_022958594.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita moschata])

HSP 1 Score: 332.0 bits (850), Expect = 3.1e-87
Identity = 159/178 (89.33%), Postives = 166/178 (93.26%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MGFPASYTELLLPKL IHV S LGFLRKLISLLF LLGLQDFLEPDIPW NH DSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  FADLDPLSAAVLREILPVIKFSELLDPPESCAVCLYEFEAEDEIRRLANCRHIFHRGCLD 120
             DLDPLSAAVLREILPV++FSELLDPPESCAVCLYEFE +DEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALLSDSSSSS-LLAAGL 178
           RWIGYGQRTCPLCRTVFIPPDLR+GGCNFDDR WEDSE+FEAL +DSSSSS LL+AGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRAWEDSELFEALHTDSSSSSTLLSAGL 178

[Close]

BLAST of ClCG01G002500 vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 4.7e-46
Identity = 90/165 (54.55%), Postives = 112/165 (67.88%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MGFP  YTE+ LPKL +   S LGF+R ++  +FR LGL DFLE D  WP++  S+PT  
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDY-TSYPTRI 60

Query: 61  FADLDPLSAAVLREILPVIKFSELL----DPPESCAVCLYEFEAEDEIRRLANCRHIFHR 120
                P SA ++REILPVIKF EL     D PE+CAVCLYEFE E EIR L NCRHIFHR
Sbjct: 61  PETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHR 120

Query: 121 GCLDRWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFE 162
            CLDRW+ + Q+TCPLCRT F+P +++     F+ RLW  S + +
Sbjct: 121 SCLDRWMDHDQKTCPLCRTPFVPDEMQE---EFNQRLWAASGVHD 161

[Close]

BLAST of ClCG01G002500 vs. ExPASy Swiss-Prot
Match: Q9SUS5 (E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 9.5e-23
Identity = 54/143 (37.76%), Postives = 82/143 (57.34%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MG P  + EL +P  V+     +GF R ++  L   +GL  FL+       H ++  + P
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLD-------HNETSRSDP 60

Query: 61  FADLDPLSAAVLREILPVIKFSELL-DPPESCAVCLYEFEAEDEIRRLANCRHIFHRGCL 120
                  SA +  E++PV++FS+LL DP + C VCL +F ++D+IR+L  C H+FH  CL
Sbjct: 61  TRLALSTSATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCL 120

Query: 121 DRWI-GYGQRTCPLCRTVFIPPD 142
           DRWI    + TCP+CR  F+P +
Sbjct: 121 DRWIVDCNKITCPICRNRFLPEE 136

[Close]

BLAST of ClCG01G002500 vs. ExPASy Swiss-Prot
Match: Q9SUS4 (Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RHA1A PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.4e-21
Identity = 52/144 (36.11%), Postives = 83/144 (57.64%), Query Frame = 0


Query: 1   MGFPASY-TELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTG 60
           MG P  + TEL +P  ++ +   +GF R ++  L   +GL  FL+       H ++    
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLD-------HNETSAPD 60

Query: 61  PFADLDPLSAAVLREILPVIKFSEL-LDPPESCAVCLYEFEAEDEIRRLANCRHIFHRGC 120
                   SA++  E++PV++FS+L  DP + C VCL +FE++D++R+L  C H+FH  C
Sbjct: 61  LTRHALSTSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYC 120

Query: 121 LDRWI-GYGQRTCPLCRTVFIPPD 142
           LDRWI  Y +  CP+CR  F+P +
Sbjct: 121 LDRWIVDYNKMKCPVCRHRFLPKE 137

[Close]

BLAST of ClCG01G002500 vs. ExPASy Swiss-Prot
Match: Q9P7E1 (Uncharacterized RING finger protein P4H10.07 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBP4H10.07 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.9e-11
Identity = 27/47 (57.45%), Postives = 31/47 (65.96%), Query Frame = 0


Query: 89  ESCAVCLYEFEAEDEIRRLANCRHIFHRGCLDRWIGYGQRTCPLCRT 136
           E C VCL  FE  DE RRL  C H FHR C+D+W+   Q +CPLCRT
Sbjct: 523 ERCLVCLSNFELNDECRRLKQCNHFFHRECIDQWLTSSQNSCPLCRT 569

[Close]

BLAST of ClCG01G002500 vs. ExPASy Swiss-Prot
Match: O22255 (RING-H2 finger protein ATL64 OS=Arabidopsis thaliana OX=3702 GN=ATL64 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.9e-11
Identity = 37/78 (47.44%), Postives = 43/78 (55.13%), Query Frame = 0


Query: 67  LSAAVLREILPVIKFSELLDPP----ESCAVCLYEFEAEDEIRRLANCRHIFHRGCLDRW 126
           L  AVL +I P+  +S    PP    E C+VCL EFE EDE R L  C H FH  C+D W
Sbjct: 81  LDQAVLDKI-PIFVYSSKNPPPPEEKEECSVCLSEFEEEDEGRLLPKCGHSFHVDCIDTW 140

Query: 127 IGYGQRTCPLCRTVFIPP 141
               + TCPLCR    PP
Sbjct: 141 F-RSRSTCPLCRAPVQPP 156

[Close]

BLAST of ClCG01G002500 vs. ExPASy TrEMBL
Match: A0A6J1K664 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC111491048 PE=4 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 5.1e-88
Identity = 160/180 (88.89%), Postives = 167/180 (92.78%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MGFPASYTELLLPKL IHV S LGFLRKLISLLFRLLGLQDFLEPDIPW NH DSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFRLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  FADLDPLSAAVLREILPVIKFSELLDPPESCAVCLYEFEAEDEIRRLANCRHIFHRGCLD 120
             DLDPLSAAVLREILPV++FSELLDPPESCAVCLYEFE +DEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALLSDSSSSS---LLAAGL 178
           RWIGYGQRTCPLCRTVFIPPDLR+GGCNFDDR WEDSE+FEAL +DSSSSS   LL+AGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTPTLLSAGL 180

[Close]

BLAST of ClCG01G002500 vs. ExPASy TrEMBL
Match: A0A6J1I1X7 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC111469101 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 6.7e-88
Identity = 160/177 (90.40%), Postives = 165/177 (93.22%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MGFPASYTELLLPKL IHV S LGFLRKLISLLFRLLGLQDFLEPDIPWPN  DSF TGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSILGFLRKLISLLFRLLGLQDFLEPDIPWPNQSDSFTTGP 60

Query: 61  FADLDPLSAAVLREILPVIKFSELLDPPESCAVCLYEFEAEDEIRRLANCRHIFHRGCLD 120
             DLDPLSAAVLREILPV+KFSELLDPPESCAVCLYEFE++DEIR LANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFESDDEIRHLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALLSDSSSSSLLAAGL 178
           RWIGYGQRTCPLCRTVFIP DLRSG CNFDDRLWEDSEIFEAL SDSSSS+LL+AGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPGDLRSGVCNFDDRLWEDSEIFEALHSDSSSSTLLSAGL 177

[Close]

BLAST of ClCG01G002500 vs. ExPASy TrEMBL
Match: A0A6J1H5J7 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC111459775 PE=4 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.5e-87
Identity = 159/178 (89.33%), Postives = 166/178 (93.26%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MGFPASYTELLLPKL IHV S LGFLRKLISLLF LLGLQDFLEPDIPW NH DSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  FADLDPLSAAVLREILPVIKFSELLDPPESCAVCLYEFEAEDEIRRLANCRHIFHRGCLD 120
             DLDPLSAAVLREILPV++FSELLDPPESCAVCLYEFE +DEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALLSDSSSSS-LLAAGL 178
           RWIGYGQRTCPLCRTVFIPPDLR+GGCNFDDR WEDSE+FEAL +DSSSSS LL+AGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRAWEDSELFEALHTDSSSSSTLLSAGL 178

[Close]

BLAST of ClCG01G002500 vs. ExPASy TrEMBL
Match: A0A6J1EQ05 (E3 ubiquitin-protein ligase RHA1B OS=Cucurbita moschata OX=3662 GN=LOC111435580 PE=4 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.3e-87
Identity = 160/177 (90.40%), Postives = 165/177 (93.22%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MGFPASYTELLLPKL IHV S LGFLRKLISLLFRLLGLQDFLEPDI WPN  DSF TGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSVLGFLRKLISLLFRLLGLQDFLEPDILWPNQSDSFTTGP 60

Query: 61  FADLDPLSAAVLREILPVIKFSELLDPPESCAVCLYEFEAEDEIRRLANCRHIFHRGCLD 120
             DLDPLSAAVLREILPV+KFSELLDPPESCAVCLYEFE++DEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALLSDSSSSSLLAAGL 178
           RWIGYGQRTCPLCRTVFIP DLRSG CNFDDRLWEDSEIFEAL SDSSSS+LL+AGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPGDLRSGVCNFDDRLWEDSEIFEALHSDSSSSTLLSAGL 177

[Close]

BLAST of ClCG01G002500 vs. ExPASy TrEMBL
Match: A0A0A0KQV2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152910 PE=4 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 4.1e-85
Identity = 152/175 (86.86%), Postives = 163/175 (93.14%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MGFPA+YTELLLPKL IH+ SFLGFLRKLIS +FRL GLQDFLEPDIPWPN PDSFPT P
Sbjct: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFIFRLFGLQDFLEPDIPWPNPPDSFPTSP 60

Query: 61  FADLDPLSAAVLREILPVIKFSELLDPPESCAVCLYEFEAEDEIRRLANCRHIFHRGCLD 120
           F  LDPLSAA+LREILPV+KFS+LLDPP+ CAVCLYEFE++DEIRRLANCRHIFHRGCLD
Sbjct: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRS-GGCNFDDRLWEDSEIFEALLSDSSSSSLLA 175
           RWIGYGQRTCPLCRTVFIPPDLRS GGCNFDDRLWEDSEIFE L SDSSSS+LL+
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGGCNFDDRLWEDSEIFEPLHSDSSSSNLLS 175

[Close]

BLAST of ClCG01G002500 vs. TAIR 10
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2 )

HSP 1 Score: 185.7 bits (470), Expect = 3.3e-47
Identity = 90/165 (54.55%), Postives = 112/165 (67.88%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MGFP  YTE+ LPKL +   S LGF+R ++  +FR LGL DFLE D  WP++  S+PT  
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDY-TSYPTRI 60

Query: 61  FADLDPLSAAVLREILPVIKFSELL----DPPESCAVCLYEFEAEDEIRRLANCRHIFHR 120
                P SA ++REILPVIKF EL     D PE+CAVCLYEFE E EIR L NCRHIFHR
Sbjct: 61  PETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHR 120

Query: 121 GCLDRWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFE 162
            CLDRW+ + Q+TCPLCRT F+P +++     F+ RLW  S + +
Sbjct: 121 SCLDRWMDHDQKTCPLCRTPFVPDEMQE---EFNQRLWAASGVHD 161

[Close]

BLAST of ClCG01G002500 vs. TAIR 10
Match: AT1G63840.1 (RING/U-box superfamily protein )

HSP 1 Score: 183.3 bits (464), Expect = 1.7e-46
Identity = 97/173 (56.07%), Postives = 120/173 (69.36%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MGFP  Y+ELLLPK+  ++ SFLG +RKLIS +F+++GL DFLEP+    + PD  PT  
Sbjct: 1   MGFPVGYSELLLPKIFFYLLSFLGLIRKLISTMFKIIGLPDFLEPEPVSTSWPDPPPT-- 60

Query: 61  FADLDPLSAAVLR-EILPVIKFSELLDP-PESCAVCLYEFEAEDEIRRLANCRHIFHRGC 120
                P SAA+L  E+LPV++FS++  P  E CAVCLY+FE +DEIRRL NCRHIFHRGC
Sbjct: 61  --LTKPDSAAILAGEMLPVVRFSDINRPESECCAVCLYDFENDDEIRRLTNCRHIFHRGC 120

Query: 121 LDRW-IGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALLSDSSSS 171
           LDRW +GY Q TCPLCRT FIP  L+     F+ RLW  S    + L D SSS
Sbjct: 121 LDRWMMGYNQMTCPLCRTQFIPDHLQ---LEFNQRLWSQSSAVSSQLLDESSS 166

[Close]

BLAST of ClCG01G002500 vs. TAIR 10
Match: AT5G41400.1 (RING/U-box superfamily protein )

HSP 1 Score: 159.8 bits (403), Expect = 2.0e-39
Identity = 86/174 (49.43%), Postives = 109/174 (62.64%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPD-----IPWPNHPDS 60
           MG+P  YTELLLP++ +H+ S LG +R LI   FR+LGL DFLE D       W   P  
Sbjct: 1   MGYPVGYTELLLPRIFLHLLSLLGLIRTLIDTGFRILGLPDFLESDPVSSSSSWLEPPYM 60

Query: 61  FPTG----PFADLDPLSAAVLREILPVIKFSELLDP-----PESCAVCLYEFEAEDEIRR 120
                     +   P++A +  EILPVI+FSEL  P      + CAVCL+EFE +DEIRR
Sbjct: 61  STAAHHHQESSFFFPVAARLAGEILPVIRFSELTRPGFGSGSDCCAVCLHEFENDDEIRR 120

Query: 121 LANCRHIFHRGCLDRW-IGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEI 160
           L NC+HIFHR CLDRW +GY Q TCPLCRT FI  +L+     F+ R+W +SE+
Sbjct: 121 LTNCQHIFHRSCLDRWMMGYNQMTCPLCRTPFISDELQVA---FNQRVWSESEL 171

[Close]

BLAST of ClCG01G002500 vs. TAIR 10
Match: AT4G11360.1 (RING-H2 finger A1B )

HSP 1 Score: 108.2 bits (269), Expect = 6.7e-24
Identity = 54/143 (37.76%), Postives = 82/143 (57.34%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60
           MG P  + EL +P  V+     +GF R ++  L   +GL  FL+       H ++  + P
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLD-------HNETSRSDP 60

Query: 61  FADLDPLSAAVLREILPVIKFSELL-DPPESCAVCLYEFEAEDEIRRLANCRHIFHRGCL 120
                  SA +  E++PV++FS+LL DP + C VCL +F ++D+IR+L  C H+FH  CL
Sbjct: 61  TRLALSTSATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCL 120

Query: 121 DRWI-GYGQRTCPLCRTVFIPPD 142
           DRWI    + TCP+CR  F+P +
Sbjct: 121 DRWIVDCNKITCPICRNRFLPEE 136

[Close]

BLAST of ClCG01G002500 vs. TAIR 10
Match: AT4G11370.1 (RING-H2 finger A1A )

HSP 1 Score: 104.4 bits (259), Expect = 9.7e-23
Identity = 52/144 (36.11%), Postives = 83/144 (57.64%), Query Frame = 0


Query: 1   MGFPASY-TELLLPKLVIHVFSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTG 60
           MG P  + TEL +P  ++ +   +GF R ++  L   +GL  FL+       H ++    
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLD-------HNETSAPD 60

Query: 61  PFADLDPLSAAVLREILPVIKFSEL-LDPPESCAVCLYEFEAEDEIRRLANCRHIFHRGC 120
                   SA++  E++PV++FS+L  DP + C VCL +FE++D++R+L  C H+FH  C
Sbjct: 61  LTRHALSTSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYC 120

Query: 121 LDRWI-GYGQRTCPLCRTVFIPPD 142
           LDRWI  Y +  CP+CR  F+P +
Sbjct: 121 LDRWIVDYNKMKCPVCRHRFLPKE 137

The following BLAST results are available for this feature:

BLAST of ClCG01G002500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906771.1	3.8e-93	93.89	E3 ubiquitin-protein ligase RHA1B [Benincasa hispida]	[more]
KAG6579522.1	4.8e-88	90.96	Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp...	[more]
XP_022995549.1	1.1e-87	88.89	E3 ubiquitin-protein ligase RHA1B-like [Cucurbita maxima] >XP_023533861.1 E3 ubi...	[more]
XP_022970110.1	1.4e-87	90.40	E3 ubiquitin-protein ligase RHA1B-like [Cucurbita maxima]	[more]
XP_022958594.1	3.1e-87	89.33	E3 ubiquitin-protein ligase RHA1B-like [Cucurbita moschata]	[more]

BLAST of ClCG01G002500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XF92	4.7e-46	54.55	Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH...	[more]
Q9SUS5	9.5e-23	37.76	E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 ...	[more]
Q9SUS4	1.4e-21	36.11	Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q9P7E1	2.9e-11	57.45	Uncharacterized RING finger protein P4H10.07 OS=Schizosaccharomyces pombe (strai...	[more]
O22255	4.9e-11	47.44	RING-H2 finger protein ATL64 OS=Arabidopsis thaliana OX=3702 GN=ATL64 PE=2 SV=1	[more]

BLAST of ClCG01G002500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K664	5.1e-88	88.89	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC1114910...	[more]
A0A6J1I1X7	6.7e-88	90.40	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC1114691...	[more]
A0A6J1H5J7	1.5e-87	89.33	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1EQ05	3.3e-87	90.40	E3 ubiquitin-protein ligase RHA1B OS=Cucurbita moschata OX=3662 GN=LOC111435580 ...	[more]
A0A0A0KQV2	4.1e-85	86.86	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152910 P...	[more]

BLAST of ClCG01G002500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G61460.1	3.3e-47	54.55	brassinosteroid-responsive RING-H2	[more]
AT1G63840.1	1.7e-46	56.07	RING/U-box superfamily protein	[more]
AT5G41400.1	2.0e-39	49.43	RING/U-box superfamily protein	[more]
AT4G11360.1	6.7e-24	37.76	RING-H2 finger A1B	[more]
AT4G11370.1	9.7e-23	36.11	RING-H2 finger A1A	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 91..133 e-value: 3.3E-6 score: 36.6
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 90..134 e-value: 1.6E-13 score: 50.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 91..134 score: 12.459772
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 90..134 e-value: 0.0081 score: 7.9
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 85..137 e-value: 9.8E-18 score: 65.5
None	No IPR available	PANTHER	PTHR45969	RING ZINC FINGER PROTEIN-RELATED	coord: 1..144
None	No IPR available	PANTHER	PTHR45969:SF49	SUBFAMILY NOT NAMED	coord: 1..144
None	No IPR available	CDD	cd16448	RING-H2	coord: 91..134 e-value: 2.55746E-13 score: 59.3879
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 72..140

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG01G002500.1	ClCG01G002500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding