ChyUNG230300 (gene) Cucumber (hystrix) v1

Overview
NameChyUNG230300
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionL-galactono-1,4-lactone dehydrogenase
Locationscaffold32_size1584725: 997132 .. 1001928 (+)
RNA-Seq ExpressionChyUNG230300
SyntenyChyUNG230300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCAACTTTCTCTCTCTCCGGCGATCCATCCATTACTTCCGCCACCGCCGTCTGCAAATCTCACCCACCGCCGCCATTTCCAAACCTCCTTTCAATCCCCCTCGCCCATTTTCCACTGTTTCTCCTTCACCTTCCTCCTCCTCCTCCTCCTTCGATTCCGAGCTTCGTAAGTACCTCGGCTATGGTGCTCTCGTCATTTTCTGCGGCGCCGCCACTTACTACTCCTTCCCTTTTCCCGAAAACGCCAAGCACAAGAAGGCCCAGATCTTCCGATACACTCCACTGCCTGAGGATCTTCACACTGTGTCCAATTGGAGTGGAACTCATGAGGTTTGGACCAGAGTGTTTCACCAGCCGGAGGATGTGGAGCAGCTTGAGCAAGTGGTGAAACAGGCTAATGAGAAGAAGGCTAGGATTCGGCCTGTTGGCTCCGGGCTTTCGCCTAATGGGATTGGATTATCGAGGATGGGGATGGTGAATTTGGCGTTGATGGATAAAGTTTTGGAAGTAGATAAGGAGAAGAAGAGGGTTAGGGTTCAAGCGGGAATAAGGGTTCAGCAGCTTGTGGATGGAATCAAGGAGTATGGGCTTACTTTGCAGAATTTTGCTTCTATCAGGGAGCAGCAGATTGGTGGCATCATTCAGGTGGTTATATGAGCATTTCTGTGTTTTTCATGTTGTTCACTTGCTATTAGAATTTTGATCTGTTTGATAACTTGAGCATCAATGTCATGGCTGCGTGTTGATAAATATGAACAATCACCTATCCAGGGTGTACATTAGTGTTTTGAGTGAAGAGAAAAAAAAGAATACCATTGTTTCTTGTCTTAAATAGATTGAAAGTGATTAGATTTGATATGCAAACTTCATTTCTATCTCGATTGGCTTGTATTTTATCTCTTCTAGTGCCAAATTGTCAAGGATATGATATTTAAAGGCAGGTCGAGGTTAGTAATAGTATTTGGGGGTTACATTTGAAACAAAATTTATCTCGTTTGAAGGAGAAATGATTACATTGTTACTCCTAATCATATGAAATGAACATTGATTGCGTAGATTGGCTATTTCTTTCAGATTTTACTTTGTTTGATTGGAGTCCCTTGTTGTTTTAGTTTGGCTTTTTTGGTTGGTTGGGACTTTATTTTGTATGTCCCTCATACTCTTTTGTTTGTTCGTTATAAACAAAAAAGTAATGACTATTTTAGGTGGCATAACTATTATATGTGGTATACGATGGAAGTTTTTGATTTCAATGTTTATCTTTTCTCTTGGTTTTTTTTGCTTATGGACACTTTCACAATTATGCATCTAGGTTGGTGCACATGGTACCGGGGCAAAATTGCCTCCTATTGATGAGCAGGTTATTGCTATGAAATTGGTCACGCCTGCCAAGGGTACTATAGAGATTTCCAAAGATAAAGATCCAGAGCTTTTTTTTCTAGCTTGCTGTGGTCTTGGGGGGCTTGGAGTGGTTGCTGAAGTAACCCTCCAATGCGTTGAGAGACAGGAACTCGTGGAGCACACATATATTTCGAACATGAAAGACATCAAGAAGAACCACAAGTAATTGTGTACTTTTTTATAGTTAATTTTTATGCAGTTCTATAGGATGTTAGATTTCAGTTTACAACTCATCTTATTTATTTATTTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTTGACAATGTTTAGAGGTTAATTGAAATATTTTAGGTCCCAGTATTCTGATAATCTAAAATTTAAATACTAAATTTGAGTTATTTTGTCGCTAATACATCTTTACTCAGTTGTAGAGCTCTGAAGAGGTATATGCTCCTATCTTTGATGCTTTTATGACAAGCGTCCCACATCTTAGTGAGAGAATTACCAATTTGAGAAAGTTGAATTTTGCCATTTCTTAATGAAACATATATAATGAACAGAATCAATGGATATAAATGTAATTTTTTAATCTATAAAAGGAAACTTTCTATAGCTTGAATTTTGGGCTCTTCAAGAATGTATCATGTTGGGAATTCTAATGGATGGAAAGTGAGAATCTGATTCAGATGTTTTTCCATTTTTTTAACTTTTCATACCTTAATGTAGAGCCTTTCTTTTTTCCTCCTCCCTGAAAAACCAAGACCACAAAAACCCCTCTAGAAATAGGGTTTTCCATCTAAGTAAGATTAGAGAATAATTATAAAATGTCTTTGAGACTGAAGCTCAAGTCTGAAGCCCTGGAATTGCAAGCTATACTATTTATTGTGTTATAGCATCCAAAGGTTGTATATAGATTGGAATCCGAACTATTGATTAATGGGAGTTGTTGCTTAATGTAGATTTGTACTATTAATTTCAAATTGTCTAGTTTTGAACAAGTCTTCTCTTTTGGATCTAAACTAAATTGAATCCTTTCTCTCTACCATCCACGTCACTCACAAATAGGATGAAACTTTTTTATTGTCTTCATCCTCTAAAGTCAAGTCACCCAAGTAATGTTGAAGCATTACACATATAACGACTGATATTCTCTTTATGCAGGAAATTATTGGCTGACAATAAGCATGTGAAATACCTGTACATTCCTTACACCGATGCTATTGTTGTTGTAACTTGCAATCCTATCTCAAAATGGAGAGGCCCTCCCAAATTCAAACCAAAATATACAAGTGAGGAAGCCATACAGCATGTTCGTGATCTCTACGTGGAGTCTCTTAAAAAATACAGGTTACTTCCATCTTCTGTTTGAGTATATTTAGTCGTCAATAATTTTAGAATTAGGACACTGGAAATGGGTTGAAACATAGTTGACATATTTATCCTCTCAAATCACTTTTCATTCCTTTTGATTTGTTTCTCCTTCTCTTTTGGTTGGTATTCATTTTAATCAAAGAGTCATTTGAGGCCTTTTTCAAACTTTTTGCTATTTATATTTTTCTTACCAATTTATTTTCCCAGTGCTTTTTATTTCATCTAATTGCTTGCTTTATAATTGCAGTGCTAGTGAGGAAAGAGACATAAATGAATTTTCATTTACTGAGTTGCGCGATAAACTACTTGCCCTTGATCCTCTTAACAAAGAGCATGTCGTAAAAGTGAATCAAACGGAGGCTGAGTTCTGGAGAAAGTCAGAAGGATACAGAGTCGGATGGAGTGATGAAATCTTGGGTTTTGATTGTGGTGGCCAACAATGGGTCTCTGAGACTTGCTTTCCAGCCGGTACTCTTGCAAAACCTAACATGAAAGACATTGAATACATAGAGGAATTGAAGCAACTTATTGAAAAAGAAAACATACCTGCGCCTGCTCCTCTTGAGCAGCGGTGGACTGCTCGTAGCAAGAGTCCCATGAGTCCAGCTTCAAGCACAGCTGAGGATGATATATTCTCTTGGGTAAACTCTAAATTTCAAACCTTATACTCTCTCTTTCAAGTTTTAAATCTATACCTGTGCAGCCCCCAAGGTTGCCCTGGTGATAAAGACAGGGATGTTTAGAGGTATCCTCCAAGATTTTGGAACATAATTACTTAAAACAATCCTTGTTCTTTTGGAGTTGGTATTGGGGCAGGCGAGCTTCATAACCACGTGTATAATGGAAAGAGTATCCCCCCATGTTGGTTAAAAATTGACTTTCTATACTTGTGCTTTGTGCATGAAGGGAGAAGCATAAGTTATATTTTTGAGAAATAGTAAAACTAAATACCAAGTATTATATAGTTTCTTTTCTGCTAAATGGGTCGTATATTCTAAAGAGTGTTGTGATCTTATATGATGATTAGTTGCTGAAGAGTGTGTACTCAAACTCTTTTTTTTTTCAGTAGCTTCCGGTCTTTACCATCTCAAAGCACATCACCTTAGTTTGGCGGATGTGTCTGTATCCAACCTGTGTCAAACACTTGCTGTACACGTATCAAACACTTGTTAGTACAATACATGTGTTAATACAAAGTCCAAATAAGGACTGATATTTATTAGACATGCATCAAATATTTGTTTCATATACTAAAGCTAAACAGACACATAAATGACAAAAAGAAAAAAAATTTGAAAGCAAAATACTGAATTTTTAAGCATATAAATGCCTAACTTATTGACTTTGATGTACTTGTATAAAATTTTATATATTTTAAAGAAGTATGTTTTAACCTTTAGTACATATGTTCTTGTTGTGTTTATGTCCTTAAATTAAAAAAACAATGTGTCGCCATGTTCTTGTTGCATCAGTGTGGTGTTGTGTCACATCCGTATCTGTGCTTCTCGACAGAAATCTTTACTCAATTGCCAACAGATTTTATCTTGATGTCTCACAAATTAGAACTCAGTAGACCCAACTGTGGTTTCTTCTTTGTTAATCTTGTTAATTTGATTGATTGTCTTTTGCTGCGATTAGGTCGGAATAATCATGTACCTACCTACGTCAGATGCGCGCCAGAGAAAGGAAATCACAGAGGAGTTCTTTCACTATAGGCACCTCACCCAAACACTGCTATGGGATCAATATTCTGCCTTTGAACATTGGGCTAAGATCGAGGTACCCAATTTCAAAATCTTTTCATGATTAAGCCCTACAAAAATGGGGGCATAAAGTTTTGACCACTTTGTTATATTCTATGTAAAATCCAGGTTCCCAAGGACAAGGATGAGCTTGCAGCTCTGCAAGCAAGACTTAGGAAACGGTTTCCTGTTGACGAATACAACAAGGCACGACGGGCACTTGACCCGAACAAAATTCTTTCAAATAACAAGTTGGAAACACTGTTCTCGTCAACAGATACGGTTTGA

mRNA sequence

ATGCTCAACTTTCTCTCTCTCCGGCGATCCATCCATTACTTCCGCCACCGCCGTCTGCAAATCTCACCCACCGCCGCCATTTCCAAACCTCCTTTCAATCCCCCTCGCCCATTTTCCACTGTTTCTCCTTCACCTTCCTCCTCCTCCTCCTCCTTCGATTCCGAGCTTCGTAAGTACCTCGGCTATGGTGCTCTCGTCATTTTCTGCGGCGCCGCCACTTACTACTCCTTCCCTTTTCCCGAAAACGCCAAGCACAAGAAGGCCCAGATCTTCCGATACACTCCACTGCCTGAGGATCTTCACACTGTGTCCAATTGGAGTGGAACTCATGAGGTTTGGACCAGAGTGTTTCACCAGCCGGAGGATGTGGAGCAGCTTGAGCAAGTGGTGAAACAGGCTAATGAGAAGAAGGCTAGGATTCGGCCTGTTGGCTCCGGGCTTTCGCCTAATGGGATTGGATTATCGAGGATGGGGATGGTGAATTTGGCGTTGATGGATAAAGTTTTGGAAGTAGATAAGGAGAAGAAGAGGGTTAGGGTTCAAGCGGGAATAAGGGTTCAGCAGCTTGTGGATGGAATCAAGGAGTATGGGCTTACTTTGCAGAATTTTGCTTCTATCAGGGAGCAGCAGATTGGTGGCATCATTCAGGTTGGTGCACATGGTACCGGGGCAAAATTGCCTCCTATTGATGAGCAGGTTATTGCTATGAAATTGGTCACGCCTGCCAAGGGTACTATAGAGATTTCCAAAGATAAAGATCCAGAGCTTTTTTTTCTAGCTTGCTGTGGTCTTGGGGGGCTTGGAGTGGTTGCTGAAGTAACCCTCCAATGCGTTGAGAGACAGGAACTCGTGGAGCACACATATATTTCGAACATGAAAGACATCAAGAAGAACCACAAGAAATTATTGGCTGACAATAAGCATGTGAAATACCTGTACATTCCTTACACCGATGCTATTGTTGTTGTAACTTGCAATCCTATCTCAAAATGGAGAGGCCCTCCCAAATTCAAACCAAAATATACAAGTGAGGAAGCCATACAGCATGTTCGTGATCTCTACGTGGAGTCTCTTAAAAAATACAGTGCTAGTGAGGAAAGAGACATAAATGAATTTTCATTTACTGAGTTGCGCGATAAACTACTTGCCCTTGATCCTCTTAACAAAGAGCATGTCGTAAAAGTGAATCAAACGGAGGCTGAGTTCTGGAGAAAGTCAGAAGGATACAGAGTCGGATGGAGTGATGAAATCTTGGGTTTTGATTGTGGTGGCCAACAATGGGTCTCTGAGACTTGCTTTCCAGCCGGTACTCTTGCAAAACCTAACATGAAAGACATTGAATACATAGAGGAATTGAAGCAACTTATTGAAAAAGAAAACATACCTGCGCCTGCTCCTCTTGAGCAGCGGTGGACTGCTCGTAGCAAGAGTCCCATGAGTCCAGCTTCAAGCACAGCTGAGGATGATATATTCTCTTGGGTCGGAATAATCATGTACCTACCTACGTCAGATGCGCGCCAGAGAAAGGAAATCACAGAGGAGTTCTTTCACTATAGGCACCTCACCCAAACACTGCTATGGGATCAATATTCTGCCTTTGAACATTGGGCTAAGATCGAGGTTCCCAAGGACAAGGATGAGCTTGCAGCTCTGCAAGCAAGACTTAGGAAACGGTTTCCTGTTGACGAATACAACAAGGCACGACGGGCACTTGACCCGAACAAAATTCTTTCAAATAACAAGTTGGAAACACTGTTCTCGTCAACAGATACGGTTTGA

Coding sequence (CDS)

ATGCTCAACTTTCTCTCTCTCCGGCGATCCATCCATTACTTCCGCCACCGCCGTCTGCAAATCTCACCCACCGCCGCCATTTCCAAACCTCCTTTCAATCCCCCTCGCCCATTTTCCACTGTTTCTCCTTCACCTTCCTCCTCCTCCTCCTCCTTCGATTCCGAGCTTCGTAAGTACCTCGGCTATGGTGCTCTCGTCATTTTCTGCGGCGCCGCCACTTACTACTCCTTCCCTTTTCCCGAAAACGCCAAGCACAAGAAGGCCCAGATCTTCCGATACACTCCACTGCCTGAGGATCTTCACACTGTGTCCAATTGGAGTGGAACTCATGAGGTTTGGACCAGAGTGTTTCACCAGCCGGAGGATGTGGAGCAGCTTGAGCAAGTGGTGAAACAGGCTAATGAGAAGAAGGCTAGGATTCGGCCTGTTGGCTCCGGGCTTTCGCCTAATGGGATTGGATTATCGAGGATGGGGATGGTGAATTTGGCGTTGATGGATAAAGTTTTGGAAGTAGATAAGGAGAAGAAGAGGGTTAGGGTTCAAGCGGGAATAAGGGTTCAGCAGCTTGTGGATGGAATCAAGGAGTATGGGCTTACTTTGCAGAATTTTGCTTCTATCAGGGAGCAGCAGATTGGTGGCATCATTCAGGTTGGTGCACATGGTACCGGGGCAAAATTGCCTCCTATTGATGAGCAGGTTATTGCTATGAAATTGGTCACGCCTGCCAAGGGTACTATAGAGATTTCCAAAGATAAAGATCCAGAGCTTTTTTTTCTAGCTTGCTGTGGTCTTGGGGGGCTTGGAGTGGTTGCTGAAGTAACCCTCCAATGCGTTGAGAGACAGGAACTCGTGGAGCACACATATATTTCGAACATGAAAGACATCAAGAAGAACCACAAGAAATTATTGGCTGACAATAAGCATGTGAAATACCTGTACATTCCTTACACCGATGCTATTGTTGTTGTAACTTGCAATCCTATCTCAAAATGGAGAGGCCCTCCCAAATTCAAACCAAAATATACAAGTGAGGAAGCCATACAGCATGTTCGTGATCTCTACGTGGAGTCTCTTAAAAAATACAGTGCTAGTGAGGAAAGAGACATAAATGAATTTTCATTTACTGAGTTGCGCGATAAACTACTTGCCCTTGATCCTCTTAACAAAGAGCATGTCGTAAAAGTGAATCAAACGGAGGCTGAGTTCTGGAGAAAGTCAGAAGGATACAGAGTCGGATGGAGTGATGAAATCTTGGGTTTTGATTGTGGTGGCCAACAATGGGTCTCTGAGACTTGCTTTCCAGCCGGTACTCTTGCAAAACCTAACATGAAAGACATTGAATACATAGAGGAATTGAAGCAACTTATTGAAAAAGAAAACATACCTGCGCCTGCTCCTCTTGAGCAGCGGTGGACTGCTCGTAGCAAGAGTCCCATGAGTCCAGCTTCAAGCACAGCTGAGGATGATATATTCTCTTGGGTCGGAATAATCATGTACCTACCTACGTCAGATGCGCGCCAGAGAAAGGAAATCACAGAGGAGTTCTTTCACTATAGGCACCTCACCCAAACACTGCTATGGGATCAATATTCTGCCTTTGAACATTGGGCTAAGATCGAGGTTCCCAAGGACAAGGATGAGCTTGCAGCTCTGCAAGCAAGACTTAGGAAACGGTTTCCTGTTGACGAATACAACAAGGCACGACGGGCACTTGACCCGAACAAAATTCTTTCAAATAACAAGTTGGAAACACTGTTCTCGTCAACAGATACGGTTTGA

Protein sequence

MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSGTHEVWTRVFHQPEDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSASEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLETLFSSTDTV*
Homology
BLAST of ChyUNG230300 vs. ExPASy Swiss-Prot
Match: O47881 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Brassica oleracea OX=3712 PE=1 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 2.0e-266
Identity = 464/612 (75.82%), Postives = 508/612 (83.01%), Query Frame = 0

Query: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNP------------PRPFSTVSPSPSSS 60
           ML  L LRRS              A   +PPF P            P P     P P  S
Sbjct: 1   MLRSLLLRRS-------------NARSLRPPFPPLRTLCTSGQTLTPAPPPPPPPPPPIS 60

Query: 61  SSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSG 120
           SS+ + E RKY GY AL +F GAATY+SFPFPENAKHKKAQIFRY PLPEDLHTVSNWSG
Sbjct: 61  SSASEKEFRKYAGYAALALFSGAATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120

Query: 121 THEVWTRVFHQPEDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKV 180
           THEV TR F+QPE +  LE +VK+A+EKK RIRPVGSGLSPNGIGLSR GMVNLALMDKV
Sbjct: 121 THEVQTRNFNQPETLADLEALVKEAHEKKNRIRPVGSGLSPNGIGLSRSGMVNLALMDKV 180

Query: 181 LEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPP 240
           LEVDKEKKRVRVQAGIRVQQLVD I+EYGLTLQNFASIREQQIGGIIQVGAHGTGA+LPP
Sbjct: 181 LEVDKEKKRVRVQAGIRVQQLVDAIQEYGLTLQNFASIREQQIGGIIQVGAHGTGARLPP 240

Query: 241 IDEQVIAMKLVTPAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTY 300
           IDEQVI MKLVTPAKGTIE+SKD DPELF LA CGLGGLGVVAEVTLQCVERQEL+EHTY
Sbjct: 241 IDEQVIGMKLVTPAKGTIELSKDNDPELFHLARCGLGGLGVVAEVTLQCVERQELLEHTY 300

Query: 301 ISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQ 360
           +S +++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW G PK KPKYT+EEA++
Sbjct: 301 VSTLEEIKKNHKKLLSTNKHVKYLYIPYTDTVVVVTCNPVSKWSGAPKDKPKYTTEEALK 360

Query: 361 HVRDLYVESLKKYSA---------SEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTE 420
           HVRDLY ES+ KY           S E DINE SFTELRDKL+ALDPLN  HV KVNQ E
Sbjct: 361 HVRDLYRESIVKYRVQDSSKKTPDSREPDINELSFTELRDKLIALDPLNDVHVGKVNQAE 420

Query: 421 AEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKE 480
           AEFW+KSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKP+MKD+EYIE+LK+LI+KE
Sbjct: 421 AEFWKKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPSMKDLEYIEQLKELIQKE 480

Query: 481 NIPAPAPLEQRWTARSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYR 540
            IPAP+P+EQRWT RSKSPMSPA STAE+DIFSWVGIIMYLPT+D RQRK+IT+EFFHYR
Sbjct: 481 AIPAPSPIEQRWTGRSKSPMSPAFSTAEEDIFSWVGIIMYLPTADPRQRKDITDEFFHYR 540

Query: 541 HLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSN 592
           HLTQ  LWDQYSA+EHWAKIE+PKDK+EL ALQ RLRKRFPVD YNKARR LDPN+ILSN
Sbjct: 541 HLTQAKLWDQYSAYEHWAKIEIPKDKEELEALQERLRKRFPVDAYNKARRELDPNRILSN 599

BLAST of ChyUNG230300 vs. ExPASy Swiss-Prot
Match: Q9SU56 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GLDH PE=1 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 5.8e-266
Identity = 458/612 (74.84%), Postives = 515/612 (84.15%), Query Frame = 0

Query: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVS------------PSPSSS 60
           ML  L LRRS+    H    +SP+++  +  F+P R   T              P P   
Sbjct: 1   MLRSLLLRRSV---GHSLGTLSPSSSTIRSSFSPHRTLCTTGQTLTPPPPPPPRPPPPPP 60

Query: 61  SSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSG 120
           +++ +++ RKY GY AL IF G ATY+SFPFPENAKHKKAQIFRY PLPEDLHTVSNWSG
Sbjct: 61  ATASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120

Query: 121 THEVWTRVFHQPEDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKV 180
           THEV TR F+QPE++  LE +VK+++EKK RIRPVGSGLSPNGIGLSR GMVNLALMDKV
Sbjct: 121 THEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLSPNGIGLSRSGMVNLALMDKV 180

Query: 181 LEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPP 240
           LEVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIREQQIGGIIQVGAHGTGA+LPP
Sbjct: 181 LEVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIREQQIGGIIQVGAHGTGARLPP 240

Query: 241 IDEQVIAMKLVTPAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTY 300
           IDEQVI+MKLVTPAKGTIE+S++KDPELF LA CGLGGLGVVAEVTLQCV R ELVEHTY
Sbjct: 241 IDEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLGVVAEVTLQCVARHELVEHTY 300

Query: 301 ISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQ 360
           +SN+++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW GPPK KPKYT++EA+Q
Sbjct: 301 VSNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPVSKWSGPPKDKPKYTTDEAVQ 360

Query: 361 HVRDLYVESLKKYSA---------SEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTE 420
           HVRDLY ES+ KY           S E DI E SFTELRDKLLALDPLN  HV KVNQ E
Sbjct: 361 HVRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRDKLLALDPLNDVHVAKVNQAE 420

Query: 421 AEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKE 480
           AEFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA P+MKD+EYIEELK+LIEKE
Sbjct: 421 AEFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLANPSMKDLEYIEELKKLIEKE 480

Query: 481 NIPAPAPLEQRWTARSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYR 540
            IPAPAP+EQRWTARSKSP+SPA ST+EDDIFSWVGIIMYLPT+D RQRK+IT+EFFHYR
Sbjct: 481 AIPAPAPIEQRWTARSKSPISPAFSTSEDDIFSWVGIIMYLPTADPRQRKDITDEFFHYR 540

Query: 541 HLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSN 592
           HLTQ  LWDQ+SA+EHWAKIE+PKDK+EL ALQAR+RKRFPVD YNKARR LDPN+ILSN
Sbjct: 541 HLTQKQLWDQFSAYEHWAKIEIPKDKEELEALQARIRKRFPVDAYNKARRELDPNRILSN 600

BLAST of ChyUNG230300 vs. ExPASy Swiss-Prot
Match: Q2RAP0 (L-galactono-1,4-lactone dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GLDH1 PE=2 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 1.9e-240
Identity = 406/558 (72.76%), Postives = 475/558 (85.13%), Query Frame = 0

Query: 36  RPFSTVSPSPSSSSSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTP 95
           R  S  SP P+S     D++LRKY GY  L++ CGAATYYSFP P +A HKKA  F+Y P
Sbjct: 27  RALSASSPLPAS-----DADLRKYAGYALLLLGCGAATYYSFPLPPDALHKKAVPFKYAP 86

Query: 96  LPEDLHTVSNWSGTHEVWTRVFHQPEDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLS 155
           LP+DLH VSNWS THEV TRV  QP+ +  L   +  A+ +  ++RP+GSGLSPNG+ LS
Sbjct: 87  LPDDLHAVSNWSATHEVHTRVLLQPDSLPALHDALAAAHGECRKLRPLGSGLSPNGLALS 146

Query: 156 RMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGII 215
           R GMVNLALMDKVL VD +KK V VQAGIRV +LVD ++E+GLTLQNFASIREQQ+GGII
Sbjct: 147 RAGMVNLALMDKVLGVDAKKKTVTVQAGIRVAELVDALREHGLTLQNFASIREQQVGGII 206

Query: 216 QVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTL 275
           QVGAHGTGA+LPPIDEQVI+MKLVTPAKGTIE+S++KDP+LF+LA CGLGGLGVVAEVTL
Sbjct: 207 QVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPDLFYLARCGLGGLGVVAEVTL 266

Query: 276 QCVERQELVEHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPP 335
           QCVER +L+EHT++SN  ++KKNHKK L++NKH+KYL+IPYTD +VVV CNP S+WR  P
Sbjct: 267 QCVERHQLIEHTFVSNADEVKKNHKKWLSENKHIKYLWIPYTDTVVVVQCNPPSRWR-TP 326

Query: 336 KFKPKYTSEEAIQHVRDLYVESLKKY---SASEERDINEFSFTELRDKLLALDPLNKEHV 395
           KF  KY  +EAIQHVRDLY ESLKKY   + S + ++++ SFTELRD+LL LDPL+K+HV
Sbjct: 327 KFTSKYGKDEAIQHVRDLYHESLKKYRTKAESNDPEVDQLSFTELRDRLLTLDPLDKDHV 386

Query: 396 VKVNQTEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEEL 455
           +++N+ EAE+W+KSEGYR+GWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKD++YIEEL
Sbjct: 387 IRINKAEAEYWKKSEGYRMGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDLDYIEEL 446

Query: 456 KQLIEKENIPAPAPLEQRWTARSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEIT 515
            QLIEKE+IPAPAP+EQRWTA S+SPMSPASS+ EDDIFSWVGIIMYLPTSDARQRKEIT
Sbjct: 447 LQLIEKEDIPAPAPIEQRWTACSRSPMSPASSSQEDDIFSWVGIIMYLPTSDARQRKEIT 506

Query: 516 EEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARRALD 575
           EEFF+YR  TQT LWD YSA+EHWAKIEVPKDKDELA LQARLRKRFPVD YNKAR  LD
Sbjct: 507 EEFFNYRSKTQTNLWDGYSAYEHWAKIEVPKDKDELAELQARLRKRFPVDAYNKARMELD 566

Query: 576 PNKILSNNKLETLFSSTD 591
           PNK+LSN KLE LF  T+
Sbjct: 567 PNKVLSNAKLEKLFPVTE 578

BLAST of ChyUNG230300 vs. ExPASy Swiss-Prot
Match: Q2QXY1 (L-galactono-1,4-lactone dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GLDH2 PE=2 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 9.3e-240
Identity = 404/558 (72.40%), Postives = 476/558 (85.30%), Query Frame = 0

Query: 36  RPFSTVSPSPSSSSSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTP 95
           R  S  SP P+S     D++LRKY GY  L++ CGAATYYSFP P +A HKKA  F+Y P
Sbjct: 27  RALSASSPLPAS-----DADLRKYAGYALLLLGCGAATYYSFPLPPDALHKKAVPFKYAP 86

Query: 96  LPEDLHTVSNWSGTHEVWTRVFHQPEDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLS 155
           LP+DLH VSNWS THEV TRV  QP+ +  L   +  A+ ++ ++RP+GSGLSPNG+ LS
Sbjct: 87  LPDDLHAVSNWSATHEVHTRVLLQPDSLPVLHDALAAAHGERRKLRPLGSGLSPNGLALS 146

Query: 156 RMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGII 215
           R GMVNLALMDKVL+VD +KK V VQAGIRV +LVD ++E+GLTLQNFASIREQQ+GGII
Sbjct: 147 RAGMVNLALMDKVLDVDAKKKTVTVQAGIRVAELVDTLREHGLTLQNFASIREQQVGGII 206

Query: 216 QVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTL 275
           QVGAHGTGA+LPPIDEQVI+MKLVTPAKGTIE+S++KDP+LF+LA CGLGGLGVVAEVTL
Sbjct: 207 QVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPDLFYLARCGLGGLGVVAEVTL 266

Query: 276 QCVERQELVEHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPP 335
           QCVER +L+EHT++S+  ++KKNHKK L++NKH+KYL+IPYTD +VVV CNP S+WR  P
Sbjct: 267 QCVERHQLIEHTFVSSADEVKKNHKKWLSENKHIKYLWIPYTDTVVVVQCNPPSRWR-TP 326

Query: 336 KFKPKYTSEEAIQHVRDLYVESLKKY---SASEERDINEFSFTELRDKLLALDPLNKEHV 395
           KF  KY  +EAIQHVRDLY ESLKKY   + S + ++++ SFTELRD+LLALDPL+K+HV
Sbjct: 327 KFTSKYGKDEAIQHVRDLYRESLKKYRTKAESNDPEVDQLSFTELRDRLLALDPLDKDHV 386

Query: 396 VKVNQTEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEEL 455
           +++N+ EAE+W+KSEGYR+GWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKD++YIEEL
Sbjct: 387 IRINKAEAEYWKKSEGYRMGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDLDYIEEL 446

Query: 456 KQLIEKENIPAPAPLEQRWTARSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEIT 515
            QLIEKE+IPAPAP+EQRWTA S+SPMSPASS+ EDDIFSWVGIIMYLPTSDARQRKEIT
Sbjct: 447 LQLIEKEDIPAPAPIEQRWTACSRSPMSPASSSQEDDIFSWVGIIMYLPTSDARQRKEIT 506

Query: 516 EEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARRALD 575
           EEFF+YR  TQT LWD YSA+EHWAKIEVPKDKDEL  L ARLRKRFPVD YNKAR  LD
Sbjct: 507 EEFFNYRSKTQTNLWDGYSAYEHWAKIEVPKDKDELTELLARLRKRFPVDAYNKARMELD 566

Query: 576 PNKILSNNKLETLFSSTD 591
           PNK+LSN KLE LF  T+
Sbjct: 567 PNKVLSNAKLEKLFPVTE 578

BLAST of ChyUNG230300 vs. ExPASy Swiss-Prot
Match: P10867 (L-gulonolactone oxidase OS=Rattus norvegicus OX=10116 GN=Gulo PE=1 SV=3)

HSP 1 Score: 152.5 bits (384), Expect = 1.5e-35
Identity = 127/484 (26.24%), Postives = 220/484 (45.45%), Query Frame = 0

Query: 105 NWSGTHEVWTRVFHQPEDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLAL 164
           NW+ T+     V++QP  VE++ +V+  A E+K +++ VG G SP+ I  +   M+++  
Sbjct: 12  NWAKTYGCSPEVYYQPTSVEEVREVLALAREQKKKVKVVGGGHSPSDIACTDGFMIHMGK 71

Query: 165 MDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGA 224
           M++VL+VDKEKK++ V+AGI +  L   + E+GL + N  ++ +  + G+I  G H TG 
Sbjct: 72  MNRVLQVDKEKKQITVEAGILLADLHPQLDEHGLAMSNLGAVSDVTVAGVIGSGTHNTGI 131

Query: 225 KLPPIDEQVIAMKLVTPAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELV 284
           K   +  QV+A+ L+T     +E S+ ++ ++F  A   LG LG++  VTLQCV +  L 
Sbjct: 132 KHGILATQVVALTLMTADGEVLECSESRNADVFQAARVHLGCLGIILTVTLQCVPQFHLQ 191

Query: 285 EHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSE 344
           E ++ S +K++  N    L  +++ ++L+ P+T+ + ++  +  +K    P     +  +
Sbjct: 192 ETSFPSTLKEVLDNLDSHLKRSEYFRFLWFPHTENVSIIYQDHTNK---APSSASNWFWD 251

Query: 345 EAIQHVRDLYVESLKKYSASEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTEAEFWR 404
            AI      ++     Y       IN F F  L                        F  
Sbjct: 252 YAIGFYLLEFLLWTSTYLPCLVGWINRFFFWML------------------------FNC 311

Query: 405 KSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKE-NIPA 464
           K E   +  S +I  ++C  +Q V +        A P  K  E + ELK ++E    + A
Sbjct: 312 KKESSNL--SHKIFTYECRFKQHVQD-------WAIPREKTKEALLELKAMLEAHPKVVA 371

Query: 465 PAPLEQRWTARSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQ 524
             P+E R+T      +SP           ++ IIMY P      R +          L  
Sbjct: 372 HYPVEVRFTRGDDILLSPCFQRDS----CYMNIIMYRPYGKDVPRLDY--------WLAY 431

Query: 525 TLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRFPV-DEYNKARRALDPNKILSNNKL 584
             +  ++    HWAK      KD          + +P   ++   R  LDP  +  N+ L
Sbjct: 432 ETIMKKFGGRPHWAKAHNCTQKD--------FEEMYPTFHKFCDIREKLDPTGMFLNSYL 439

Query: 585 ETLF 587
           E +F
Sbjct: 492 EKVF 439

BLAST of ChyUNG230300 vs. ExPASy TrEMBL
Match: E7E016 (L-galactono-1,4-lactone dehydrogenase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 579/592 (97.80%), Postives = 584/592 (98.65%), Query Frame = 0

Query: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSSSFDSELRKYL 60
           MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSP  SSSSFDSELRKYL
Sbjct: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSP--SSSSFDSELRKYL 60

Query: 61  GYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSGTHEVWTRVFHQP 120
           GYGALVIFCGAATYYSFPFPENAKHKKAQIFRY PLPEDLHTVSNWSGTHEVWTRVFHQP
Sbjct: 61  GYGALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQP 120

Query: 121 EDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV 180
           E++EQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV
Sbjct: 121 ENLEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV 180

Query: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240
           QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT
Sbjct: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240

Query: 241 PAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300
           PAKGTIEISKDKDPELF LA CGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK
Sbjct: 241 PAKGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300

Query: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK 360
           KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK
Sbjct: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK 360

Query: 361 YSASEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTEAEFWRKSEGYRVGWSDEILGF 420
           YSASEERD+NE SFTELRDKLLALDPLNKEHV+KVNQ EAEFWRKSEGYRVGWSDEILGF
Sbjct: 361 YSASEERDMNEISFTELRDKLLALDPLNKEHVIKVNQAEAEFWRKSEGYRVGWSDEILGF 420

Query: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMS 480
           DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEK+NIPAPAPLEQRWTARSKSPMS
Sbjct: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKKNIPAPAPLEQRWTARSKSPMS 480

Query: 481 PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE 540
           PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE
Sbjct: 481 PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE 540

Query: 541 VPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLETLFSSTDTV 593
           VPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLE LFSSTDTV
Sbjct: 541 VPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSTDTV 590

BLAST of ChyUNG230300 vs. ExPASy TrEMBL
Match: Q9M4U9 (L-galactono-1,4-lactone dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103497260 PE=2 SV=2)

HSP 1 Score: 1137.9 bits (2942), Expect = 0.0e+00
Identity = 567/592 (95.78%), Postives = 576/592 (97.30%), Query Frame = 0

Query: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSSSFDSELRKYL 60
           MLNFLSLRRS+HYFRHRRLQISPTAAISKPP N PRPF TVSPSP   SSSFDSELRKYL
Sbjct: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSP---SSSFDSELRKYL 60

Query: 61  GYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSGTHEVWTRVFHQP 120
           GYGALVIFCGAATYYSFPFPENAKHKKAQIFRY PLPEDLHTVSNWSGTHEV TRVFHQP
Sbjct: 61  GYGALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQP 120

Query: 121 EDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV 180
           ED+EQLEQVVKQANEKKAR+RPVGSGLSPNGIGLSR+GMVN+ALMDKVLEVDKEKKRVRV
Sbjct: 121 EDLEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRV 180

Query: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240
           QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT
Sbjct: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240

Query: 241 PAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300
           PAKGTIEIS DKDPELFFLA CGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK
Sbjct: 241 PAKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300

Query: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK 360
           KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK
Sbjct: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK 360

Query: 361 YSASEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTEAEFWRKSEGYRVGWSDEILGF 420
           YSASEERDINEFSFTELRDKLLALDPLNKEHV+KVNQ EAEFWRKSEGY+VGWSDEILGF
Sbjct: 361 YSASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYKVGWSDEILGF 420

Query: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMS 480
           DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWT RSKSPMS
Sbjct: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMS 480

Query: 481 PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE 540
           PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWD++SAFEHWAKIE
Sbjct: 481 PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIE 540

Query: 541 VPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLETLFSSTDTV 593
           VPKDKDELAALQARLRKRFPVDE NKARRALDPNKILSNNKLE LFSS D V
Sbjct: 541 VPKDKDELAALQARLRKRFPVDESNKARRALDPNKILSNNKLEKLFSSLDMV 589

BLAST of ChyUNG230300 vs. ExPASy TrEMBL
Match: A0A6J1HRG8 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111465966 PE=4 SV=1)

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 545/592 (92.06%), Postives = 564/592 (95.27%), Query Frame = 0

Query: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSSSFDSELRKYL 60
           ML  LSLRRS+H   HRR Q  PT AISKPP NP R FSTVSP P SSSS  +SELRKYL
Sbjct: 1   MLKLLSLRRSLH-LHHRRPQFPPTVAISKPPLNPTRQFSTVSPPPPSSSS--ESELRKYL 60

Query: 61  GYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSGTHEVWTRVFHQP 120
           GYGALVIFCGA TYYSFPFPENAKHKKAQIFRY PLPEDLHTVSNWSGTHEV TRVFHQP
Sbjct: 61  GYGALVIFCGATTYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVHTRVFHQP 120

Query: 121 EDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV 180
           +++EQL++VVK+AN KKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV
Sbjct: 121 DNLEQLQEVVKEANVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV 180

Query: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240
           QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT
Sbjct: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240

Query: 241 PAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300
           PAKGTIEISK+KDPELF+LA CGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK
Sbjct: 241 PAKGTIEISKEKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300

Query: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK 360
           KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKF+PKYTS+EAIQHVRDLYVESLKK
Sbjct: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKK 360

Query: 361 YSASEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTEAEFWRKSEGYRVGWSDEILGF 420
           YSAS+ERDINEFSFTELRDKLLALDPLNKEHVVKVNQ EAEFW KSEGYRVGWSDEILGF
Sbjct: 361 YSASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWWKSEGYRVGWSDEILGF 420

Query: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMS 480
           DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAP+EQRWTA SKSPMS
Sbjct: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMS 480

Query: 481 PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE 540
           PASS++EDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE
Sbjct: 481 PASSSSEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE 540

Query: 541 VPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLETLFSSTDTV 593
           VPKDKDELAALQARLRKRFPVDEYNKAR+ LDPNKILS+NKLE LF S+D V
Sbjct: 541 VPKDKDELAALQARLRKRFPVDEYNKARQELDPNKILSSNKLEKLFVSSDVV 589

BLAST of ChyUNG230300 vs. ExPASy TrEMBL
Match: A0A6J1EEF4 (L-galactono-1,4-lactone dehydrogenase, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111432481 PE=4 SV=1)

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 543/592 (91.72%), Postives = 564/592 (95.27%), Query Frame = 0

Query: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSSSFDSELRKYL 60
           ML  LSLRRS+H   HRR    PT AIS+PP NP R FSTVSP P SSSS  +SELRKY+
Sbjct: 1   MLRLLSLRRSLH-LHHRRPHFPPTVAISRPPLNPTRQFSTVSPPPPSSSS--ESELRKYI 60

Query: 61  GYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSGTHEVWTRVFHQP 120
           GYGALVI CGAATYYSFPFPENAKHKKAQIFRY PLPEDLHTVSNWSGTHEV TRVFHQP
Sbjct: 61  GYGALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQP 120

Query: 121 EDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV 180
           E++EQLE+VVK++N KKARIRPVGSGLSPNGIGLSRMGMVNLALMD+VLEVDKEKKRVRV
Sbjct: 121 ENLEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRV 180

Query: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240
           QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT
Sbjct: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240

Query: 241 PAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300
           PAKGTIEIS++KDPELF+LA CGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK
Sbjct: 241 PAKGTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300

Query: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK 360
           KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKF+PKYTS+EAIQHVRDLYVESLKK
Sbjct: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKK 360

Query: 361 YSASEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTEAEFWRKSEGYRVGWSDEILGF 420
           YSAS+ERDINEFSFTELRDKLLALDPLNKEHVVKVNQ EAEFWRKSEGYRVGWSDEILGF
Sbjct: 361 YSASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGF 420

Query: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMS 480
           DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAP+EQRWTA SKSPMS
Sbjct: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMS 480

Query: 481 PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE 540
           PASS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE
Sbjct: 481 PASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE 540

Query: 541 VPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLETLFSSTDTV 593
           VPKDKDELAALQARLRKRFPVDEYNKARR LDPNKILS+NKLE +F S+D V
Sbjct: 541 VPKDKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 589

BLAST of ChyUNG230300 vs. ExPASy TrEMBL
Match: A0A6J1DK81 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111021307 PE=4 SV=1)

HSP 1 Score: 1046.6 bits (2705), Expect = 4.0e-302
Identity = 525/595 (88.24%), Postives = 550/595 (92.44%), Query Frame = 0

Query: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFN---PPRPFSTVSPSPSSSSSSFDSELR 60
           ML  L LRRS     HRRL   P AAIS+ P N    P  FSTVSP P   SSSFDSELR
Sbjct: 1   MLRILPLRRS-----HRRLLSPPAAAISRTPLNHHHHPPSFSTVSPPP--PSSSFDSELR 60

Query: 61  KYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSGTHEVWTRVF 120
           KYLGYGALV+FCGAATYYSFPFPENAKHKKAQIFRY PLPEDLHTVSNWSGTHEV TRVF
Sbjct: 61  KYLGYGALVVFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVQTRVF 120

Query: 121 HQPEDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKR 180
            QPE++EQLEQVVK+ANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKR
Sbjct: 121 QQPENLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKR 180

Query: 181 VRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMK 240
           VRVQAGIRVQQLVDGIKE+GLTLQNFASIREQQIGGIIQVGAHGTG KLPPIDEQVIAMK
Sbjct: 181 VRVQAGIRVQQLVDGIKEHGLTLQNFASIREQQIGGIIQVGAHGTGPKLPPIDEQVIAMK 240

Query: 241 LVTPAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKK 300
           LVTPAKGTIEISK+KDPELF+LA CGLGGLGVVAEVTLQCVERQELVEHTYISNM DIKK
Sbjct: 241 LVTPAKGTIEISKEKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMNDIKK 300

Query: 301 NHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVES 360
           NHKKLL DNKHVKYLYIPYTDA+VVVTCNPISKWRGPPK KPKYTS+EAIQHVRDLY ES
Sbjct: 301 NHKKLLTDNKHVKYLYIPYTDAVVVVTCNPISKWRGPPKLKPKYTSDEAIQHVRDLYAES 360

Query: 361 LKKYSASEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTEAEFWRKSEGYRVGWSDEI 420
           LKKYS S+E DINE+S+TELRDKLLALDPLNKEHV KVNQ EAEFWRKSEGY+VGWSDEI
Sbjct: 361 LKKYSTSDETDINEYSYTELRDKLLALDPLNKEHVTKVNQAEAEFWRKSEGYKVGWSDEI 420

Query: 421 LGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKS 480
           LGFDCGGQQWVSETCFPAGTLAKP+MKDI+YIE+LKQLIEKENIPAPAP+EQRWTA SKS
Sbjct: 421 LGFDCGGQQWVSETCFPAGTLAKPSMKDIKYIEDLKQLIEKENIPAPAPIEQRWTACSKS 480

Query: 481 PMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWA 540
           PMSPASS A+DD+FSWVGIIMYLPT DARQRKEITEEFFHYRHLTQ+ LWDQYSAFEHWA
Sbjct: 481 PMSPASSLAQDDLFSWVGIIMYLPTLDARQRKEITEEFFHYRHLTQSQLWDQYSAFEHWA 540

Query: 541 KIEVPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLETLFSSTDTV 593
           KIEVP+DK+ELAALQARLRKRFPVDE+NKA+RALDPNKILSNNKLE LF S D V
Sbjct: 541 KIEVPRDKEELAALQARLRKRFPVDEFNKAQRALDPNKILSNNKLEKLFPSNDAV 588

BLAST of ChyUNG230300 vs. NCBI nr
Match: NP_001292704.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis sativus] >ADT80722.1 L-galactono-1,4-lactone dehydrogenase [Cucumis sativus])

HSP 1 Score: 1157 bits (2994), Expect = 0.0
Identity = 579/592 (97.80%), Postives = 584/592 (98.65%), Query Frame = 0

Query: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSSSFDSELRKYL 60
           MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPS  SSSFDSELRKYL
Sbjct: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPS--SSSFDSELRKYL 60

Query: 61  GYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSGTHEVWTRVFHQP 120
           GYGALVIFCGAATYYSFPFPENAKHKKAQIFRY PLPEDLHTVSNWSGTHEVWTRVFHQP
Sbjct: 61  GYGALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQP 120

Query: 121 EDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV 180
           E++EQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV
Sbjct: 121 ENLEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV 180

Query: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240
           QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT
Sbjct: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240

Query: 241 PAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300
           PAKGTIEISKDKDPELF LA CGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK
Sbjct: 241 PAKGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300

Query: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK 360
           KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK
Sbjct: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK 360

Query: 361 YSASEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTEAEFWRKSEGYRVGWSDEILGF 420
           YSASEERD+NE SFTELRDKLLALDPLNKEHV+KVNQ EAEFWRKSEGYRVGWSDEILGF
Sbjct: 361 YSASEERDMNEISFTELRDKLLALDPLNKEHVIKVNQAEAEFWRKSEGYRVGWSDEILGF 420

Query: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMS 480
           DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEK+NIPAPAPLEQRWTARSKSPMS
Sbjct: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKKNIPAPAPLEQRWTARSKSPMS 480

Query: 481 PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE 540
           PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE
Sbjct: 481 PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE 540

Query: 541 VPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLETLFSSTDTV 592
           VPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLE LFSSTDTV
Sbjct: 541 VPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSTDTV 590

BLAST of ChyUNG230300 vs. NCBI nr
Match: KAE8637410.1 (hypothetical protein CSA_023387 [Cucumis sativus])

HSP 1 Score: 1148 bits (2969), Expect = 0.0
Identity = 573/585 (97.95%), Postives = 578/585 (98.80%), Query Frame = 0

Query: 8   RRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSSSFDSELRKYLGYGALVI 67
           RRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPS  SSSFDSELRKYLGYGALVI
Sbjct: 240 RRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPS--SSSFDSELRKYLGYGALVI 299

Query: 68  FCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSGTHEVWTRVFHQPEDVEQLE 127
           FCGAATYYSFPFPENAKHKKAQIFRY PLPEDLHTVSNWSGTHEVWTRVFHQPE++EQLE
Sbjct: 300 FCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPENLEQLE 359

Query: 128 QVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQ 187
           QVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQ
Sbjct: 360 QVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQ 419

Query: 188 QLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIE 247
           QLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIE
Sbjct: 420 QLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIE 479

Query: 248 ISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNK 307
           ISKDKDPELF LA CGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNK
Sbjct: 480 ISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNK 539

Query: 308 HVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSASEER 367
           HVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSASEER
Sbjct: 540 HVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSASEER 599

Query: 368 DINEFSFTELRDKLLALDPLNKEHVVKVNQTEAEFWRKSEGYRVGWSDEILGFDCGGQQW 427
           D+NEFSFTELRDKLLALDPLNKEHV+KVNQ EAEFWRKSEGYRVGWSDEILGFDCGGQQW
Sbjct: 600 DMNEFSFTELRDKLLALDPLNKEHVIKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQW 659

Query: 428 VSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMSPASSTAE 487
           VSETCFPAGTLAKPNMKDIEYIEELKQLIEK+NIPAPAPLEQRWTARSKSPMSPASSTAE
Sbjct: 660 VSETCFPAGTLAKPNMKDIEYIEELKQLIEKKNIPAPAPLEQRWTARSKSPMSPASSTAE 719

Query: 488 DDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDE 547
           DDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDE
Sbjct: 720 DDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDE 779

Query: 548 LAALQARLRKRFPVDEYNKARRALDPNKILSNNKLETLFSSTDTV 592
           LAALQARLRKRFPVDEYNKARRALDPNKILSNNKLE LFSSTDTV
Sbjct: 780 LAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSTDTV 822

BLAST of ChyUNG230300 vs. NCBI nr
Match: NP_001284400.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis melo] >AAF64319.2 L-galactono-1,4-lactone dehydrogenase [Cucumis melo])

HSP 1 Score: 1133 bits (2931), Expect = 0.0
Identity = 567/592 (95.78%), Postives = 576/592 (97.30%), Query Frame = 0

Query: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSSSFDSELRKYL 60
           MLNFLSLRRS+HYFRHRRLQISPTAAISKPP N PRPF TVSPSP   SSSFDSELRKYL
Sbjct: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSP---SSSFDSELRKYL 60

Query: 61  GYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSGTHEVWTRVFHQP 120
           GYGALVIFCGAATYYSFPFPENAKHKKAQIFRY PLPEDLHTVSNWSGTHEV TRVFHQP
Sbjct: 61  GYGALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQP 120

Query: 121 EDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV 180
           ED+EQLEQVVKQANEKKAR+RPVGSGLSPNGIGLSR+GMVN+ALMDKVLEVDKEKKRVRV
Sbjct: 121 EDLEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRV 180

Query: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240
           QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT
Sbjct: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240

Query: 241 PAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300
           PAKGTIEIS DKDPELFFLA CGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK
Sbjct: 241 PAKGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300

Query: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK 360
           KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK
Sbjct: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK 360

Query: 361 YSASEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTEAEFWRKSEGYRVGWSDEILGF 420
           YSASEERDINEFSFTELRDKLLALDPLNKEHV+KVNQ EAEFWRKSEGY+VGWSDEILGF
Sbjct: 361 YSASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYKVGWSDEILGF 420

Query: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMS 480
           DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWT RSKSPMS
Sbjct: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMS 480

Query: 481 PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE 540
           PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWD++SAFEHWAKIE
Sbjct: 481 PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIE 540

Query: 541 VPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLETLFSSTDTV 592
           VPKDKDELAALQARLRKRFPVDE NKARRALDPNKILSNNKLE LFSS D V
Sbjct: 541 VPKDKDELAALQARLRKRFPVDESNKARRALDPNKILSNNKLEKLFSSLDMV 589

BLAST of ChyUNG230300 vs. NCBI nr
Match: XP_038882838.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 1096 bits (2835), Expect = 0.0
Identity = 551/592 (93.07%), Postives = 565/592 (95.44%), Query Frame = 0

Query: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSSSFDSELRKYL 60
           ML  L+LRRS H+ R R LQI PT AISKPP NPPRPFSTVSP P   SSSFDSELRKYL
Sbjct: 1   MLKLLALRRS-HFCRLRGLQIPPTTAISKPPLNPPRPFSTVSPPPQ--SSSFDSELRKYL 60

Query: 61  GYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSGTHEVWTRVFHQP 120
           GYG LVI CGAATYYSFPFPENAKHKKAQIFRY PLPEDLHTVSNWSGTHEV TRVFHQP
Sbjct: 61  GYGTLVIICGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVQTRVFHQP 120

Query: 121 EDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV 180
           E++EQLEQVVK+ANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMD VLEVDK++KRVRV
Sbjct: 121 ENLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDNVLEVDKDRKRVRV 180

Query: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240
           QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT
Sbjct: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240

Query: 241 PAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300
           PAKGTIEISKDKDPELFFLA CGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK
Sbjct: 241 PAKGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300

Query: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK 360
           KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPK KPKYTSEEAIQHVRDLYVESLKK
Sbjct: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKLKPKYTSEEAIQHVRDLYVESLKK 360

Query: 361 YSASEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTEAEFWRKSEGYRVGWSDEILGF 420
           +S S+E DINEFSFTELRDKLLALDPLNKEHV+KVNQ EAEFW+KSEGYRVGWSDEILGF
Sbjct: 361 HSVSDETDINEFSFTELRDKLLALDPLNKEHVIKVNQAEAEFWKKSEGYRVGWSDEILGF 420

Query: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMS 480
           DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAP+EQRWTA SKSPMS
Sbjct: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMS 480

Query: 481 PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE 540
           PASS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHL QTLLWDQYSAFEHWAKIE
Sbjct: 481 PASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLAQTLLWDQYSAFEHWAKIE 540

Query: 541 VPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLETLFSSTDTV 592
           VPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLE L SS+DTV
Sbjct: 541 VPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLLSSSDTV 589

BLAST of ChyUNG230300 vs. NCBI nr
Match: XP_023517263.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1088 bits (2813), Expect = 0.0
Identity = 548/592 (92.57%), Postives = 564/592 (95.27%), Query Frame = 0

Query: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSSSFDSELRKYL 60
           ML  LSLRRS+H   HRR Q  PT AISKPP NP R FSTVSP P SSSS  +SELRKYL
Sbjct: 1   MLKLLSLRRSLH-LHHRRPQFPPTVAISKPPLNPTRQFSTVSPPPPSSSS--ESELRKYL 60

Query: 61  GYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSGTHEVWTRVFHQP 120
           GYGALVI CGAATYYSFPFPENAKHKKAQIFRY PLPEDLHTVSNWSGTHEV TRVFHQP
Sbjct: 61  GYGALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQP 120

Query: 121 EDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV 180
           E++EQLE+VVK++N KKARIRPVGSGLSPNGIGLSRMGMVNLALMD+VLEVDKEKKRVRV
Sbjct: 121 ENLEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDRVLEVDKEKKRVRV 180

Query: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240
           QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT
Sbjct: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240

Query: 241 PAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300
           PAKGTIEISKDKDPELF+LA CGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK
Sbjct: 241 PAKGTIEISKDKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300

Query: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKK 360
           KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKF+PKYTS+EAIQHVRDLYVESLKK
Sbjct: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKK 360

Query: 361 YSASEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTEAEFWRKSEGYRVGWSDEILGF 420
           YSAS+ERDINEFSFTELRDKLLALDPLNKEHVVKVNQ EAEFWRKSEGYRVGWSDEILGF
Sbjct: 361 YSASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGF 420

Query: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTARSKSPMS 480
           DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAP+EQRWTA SKSPMS
Sbjct: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMS 480

Query: 481 PASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE 540
           PASS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE
Sbjct: 481 PASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE 540

Query: 541 VPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLETLFSSTDTV 592
           VPKDKDELAALQARLRKRFPVDEYNKAR  LDPNKILS+NKLE LF S+D V
Sbjct: 541 VPKDKDELAALQARLRKRFPVDEYNKARGELDPNKILSSNKLEKLFVSSDVV 589

BLAST of ChyUNG230300 vs. TAIR 10
Match: AT3G47930.1 (L-galactono-1,4-lactone dehydrogenase )

HSP 1 Score: 917.9 bits (2371), Expect = 4.1e-267
Identity = 458/612 (74.84%), Postives = 515/612 (84.15%), Query Frame = 0

Query: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVS------------PSPSSS 60
           ML  L LRRS+    H    +SP+++  +  F+P R   T              P P   
Sbjct: 1   MLRSLLLRRSV---GHSLGTLSPSSSTIRSSFSPHRTLCTTGQTLTPPPPPPPRPPPPPP 60

Query: 61  SSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSG 120
           +++ +++ RKY GY AL IF G ATY+SFPFPENAKHKKAQIFRY PLPEDLHTVSNWSG
Sbjct: 61  ATASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120

Query: 121 THEVWTRVFHQPEDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKV 180
           THEV TR F+QPE++  LE +VK+++EKK RIRPVGSGLSPNGIGLSR GMVNLALMDKV
Sbjct: 121 THEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLSPNGIGLSRSGMVNLALMDKV 180

Query: 181 LEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPP 240
           LEVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIREQQIGGIIQVGAHGTGA+LPP
Sbjct: 181 LEVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIREQQIGGIIQVGAHGTGARLPP 240

Query: 241 IDEQVIAMKLVTPAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTY 300
           IDEQVI+MKLVTPAKGTIE+S++KDPELF LA CGLGGLGVVAEVTLQCV R ELVEHTY
Sbjct: 241 IDEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLGVVAEVTLQCVARHELVEHTY 300

Query: 301 ISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQ 360
           +SN+++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW GPPK KPKYT++EA+Q
Sbjct: 301 VSNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPVSKWSGPPKDKPKYTTDEAVQ 360

Query: 361 HVRDLYVESLKKYSA---------SEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTE 420
           HVRDLY ES+ KY           S E DI E SFTELRDKLLALDPLN  HV KVNQ E
Sbjct: 361 HVRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRDKLLALDPLNDVHVAKVNQAE 420

Query: 421 AEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKE 480
           AEFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA P+MKD+EYIEELK+LIEKE
Sbjct: 421 AEFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLANPSMKDLEYIEELKKLIEKE 480

Query: 481 NIPAPAPLEQRWTARSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYR 540
            IPAPAP+EQRWTARSKSP+SPA ST+EDDIFSWVGIIMYLPT+D RQRK+IT+EFFHYR
Sbjct: 481 AIPAPAPIEQRWTARSKSPISPAFSTSEDDIFSWVGIIMYLPTADPRQRKDITDEFFHYR 540

Query: 541 HLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARRALDPNKILSN 592
           HLTQ  LWDQ+SA+EHWAKIE+PKDK+EL ALQAR+RKRFPVD YNKARR LDPN+ILSN
Sbjct: 541 HLTQKQLWDQFSAYEHWAKIEIPKDKEELEALQARIRKRFPVDAYNKARRELDPNRILSN 600

BLAST of ChyUNG230300 vs. TAIR 10
Match: AT3G47930.2 (L-galactono-1,4-lactone dehydrogenase )

HSP 1 Score: 728.0 bits (1878), Expect = 6.0e-210
Identity = 367/496 (73.99%), Postives = 411/496 (82.86%), Query Frame = 0

Query: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVS------------PSPSSS 60
           ML  L LRRS+    H    +SP+++  +  F+P R   T              P P   
Sbjct: 1   MLRSLLLRRSV---GHSLGTLSPSSSTIRSSFSPHRTLCTTGQTLTPPPPPPPRPPPPPP 60

Query: 61  SSSFDSELRKYLGYGALVIFCGAATYYSFPFPENAKHKKAQIFRYTPLPEDLHTVSNWSG 120
           +++ +++ RKY GY AL IF G ATY+SFPFPENAKHKKAQIFRY PLPEDLHTVSNWSG
Sbjct: 61  ATASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120

Query: 121 THEVWTRVFHQPEDVEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKV 180
           THEV TR F+QPE++  LE +VK+++EKK RIRPVGSGLSPNGIGLSR GMVNLALMDKV
Sbjct: 121 THEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLSPNGIGLSRSGMVNLALMDKV 180

Query: 181 LEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPP 240
           LEVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIREQQIGGIIQVGAHGTGA+LPP
Sbjct: 181 LEVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIREQQIGGIIQVGAHGTGARLPP 240

Query: 241 IDEQVIAMKLVTPAKGTIEISKDKDPELFFLACCGLGGLGVVAEVTLQCVERQELVEHTY 300
           IDEQVI+MKLVTPAKGTIE+S++KDPELF LA CGLGGLGVVAEVTLQCV R ELVEHTY
Sbjct: 241 IDEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLGVVAEVTLQCVARHELVEHTY 300

Query: 301 ISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQ 360
           +SN+++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW GPPK KPKYT++EA+Q
Sbjct: 301 VSNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPVSKWSGPPKDKPKYTTDEAVQ 360

Query: 361 HVRDLYVESLKKYSA---------SEERDINEFSFTELRDKLLALDPLNKEHVVKVNQTE 420
           HVRDLY ES+ KY           S E DI E SFTELRDKLLALDPLN  HV KVNQ E
Sbjct: 361 HVRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRDKLLALDPLNDVHVAKVNQAE 420

Query: 421 AEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKE 476
           AEFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA P+MKD+EYIEELK+LIEKE
Sbjct: 421 AEFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLANPSMKDLEYIEELKKLIEKE 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O478812.0e-26675.82L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Brassica oleracea OX=371... [more]
Q9SU565.8e-26674.84L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=... [more]
Q2RAP01.9e-24072.76L-galactono-1,4-lactone dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. ja... [more]
Q2QXY19.3e-24072.40L-galactono-1,4-lactone dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. ja... [more]
P108671.5e-3526.24L-gulonolactone oxidase OS=Rattus norvegicus OX=10116 GN=Gulo PE=1 SV=3[more]
Match NameE-valueIdentityDescription
E7E0160.0e+0097.80L-galactono-1,4-lactone dehydrogenase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
Q9M4U90.0e+0095.78L-galactono-1,4-lactone dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103497260 PE... [more]
A0A6J1HRG80.0e+0092.06L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661... [more]
A0A6J1EEF40.0e+0091.72L-galactono-1,4-lactone dehydrogenase, mitochondrial-like OS=Cucurbita moschata ... [more]
A0A6J1DK814.0e-30288.24L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Momordica charantia OX=3... [more]
Match NameE-valueIdentityDescription
NP_001292704.10.097.80L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis sativus] >ADT80722... [more]
KAE8637410.10.097.95hypothetical protein CSA_023387 [Cucumis sativus][more]
NP_001284400.10.095.78L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis melo] >AAF64319.2 ... [more]
XP_038882838.10.093.07L-galactono-1,4-lactone dehydrogenase, mitochondrial isoform X1 [Benincasa hispi... [more]
XP_023517263.10.092.57L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
AT3G47930.14.1e-26774.84L-galactono-1,4-lactone dehydrogenase [more]
AT3G47930.26.0e-21073.99L-galactono-1,4-lactone dehydrogenase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 98..166
e-value: 1.5E-12
score: 48.7
IPR007173D-arabinono-1,4-lactone oxidasePFAMPF04030ALOcoord: 273..582
e-value: 8.7E-16
score: 58.4
IPR010031L-gulonolactone/D-arabinono-1,4-lactone oxidasePIRSFPIRSF000136LGO_GLOcoord: 98..590
e-value: 7.6E-60
score: 200.8
IPR010031L-gulonolactone/D-arabinono-1,4-lactone oxidasePANTHERPTHR43762L-GULONOLACTONE OXIDASEcoord: 97..587
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 116..246
e-value: 3.0E-26
score: 91.8
IPR010029Galactonolactone dehydrogenaseTIGRFAMTIGR01676TIGR01676coord: 53..587
e-value: 0.0
score: 1212.8
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 168..295
e-value: 8.1E-28
score: 98.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..49
NoneNo IPR availablePANTHERPTHR43762:SF1L-GULONOLACTONE OXIDASEcoord: 97..587
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 109..280
score: 17.170124
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 96..278

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ChyUNG230300.1ChyUNG230300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019853 L-ascorbic acid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0003885 D-arabinono-1,4-lactone oxidase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0016633 galactonolactone dehydrogenase activity
molecular_function GO:0050105 L-gulonolactone oxidase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016899 oxidoreductase activity, acting on the CH-OH group of donors, oxygen as acceptor