Chy9G171480 (gene) Cucumber (hystrix) v1

Overview
NameChy9G171480
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionSuccinate dehydrogenase assembly factor 4, mitochondrial
LocationchrH09: 15899295 .. 15899941 (-)
RNA-Seq ExpressionChy9G171480
SyntenyChy9G171480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAGCAATCTCAGCCGCCTGTTCTCGTCGCCGCCGACTCTCTCAGCCCTGCCGGCGTTGAGTCGCCTAACTTTCAACTCGTTGAGCCGATTCGTTAGCTCCTCGTCCAATCAACAGCCTTCTCGATTGGAGCAAGGTGTGTCTAGTGAGGAACAACGAGAGGCCGGGAAAGGAAGTTCGCCGAATCAACACGAGGCTGCCGGAGATGACCGAGGTGGTCAAGATGAAGGAGTTGATACGGAAAAAATGACTCGCGAGATTGGTGGACCAAAAGGCCCCGAGCCCACCCGCTACGGCGATTGGGAACGGAATGGTCGGTGCTCCGATTTTTGACATTTCGAGGTTAGATTGTGAACGAGATCTTAGGATGATATGATTTCTGTAACTCAATTTCGTGAGCTTTGTTTTCTTTAATCTGAGTTATTGTTTCGTTTTTCTGTAAATTCATTTCCAGAACTGTATGCGTTTCTTTGAGATCGAAGGGAAATTAGAATAATAGTATTCATCGTTTCAATAGTACTTGATTCTGGTAATCTTTTTGCATTACGCTTTTGTTTAGTCAGGATGTATCAAGTTCTGTCTGAACTTTGAATTTGTATAGCTTGTTGAACAAAATTTCGGAGAAATTAGAGGAAGGAGATTAG

mRNA sequence

ATGTCGAGCAATCTCAGCCGCCTGTTCTCGTCGCCGCCGACTCTCTCAGCCCTGCCGGCGTTGAGTCGCCTAACTTTCAACTCGTTGAGCCGATTCGTTAGCTCCTCGTCCAATCAACAGCCTTCTCGATTGGAGCAAGGTGTGTCTAGTGAGGAACAACGAGAGGCCGGGAAAGGAAGTTCGCCGAATCAACACGAGGCTGCCGGAGATGACCGAGGTGGTCAAGATGAAGGAGTTGATACGGAAAAAATGACTCGCGAGATTGGTGGACCAAAAGGCCCCGAGCCCACCCGCTACGGCGATTGGGAACGGAATGGTCGCTTGTTGAACAAAATTTCGGAGAAATTAGAGGAAGGAGATTAG

Coding sequence (CDS)

ATGTCGAGCAATCTCAGCCGCCTGTTCTCGTCGCCGCCGACTCTCTCAGCCCTGCCGGCGTTGAGTCGCCTAACTTTCAACTCGTTGAGCCGATTCGTTAGCTCCTCGTCCAATCAACAGCCTTCTCGATTGGAGCAAGGTGTGTCTAGTGAGGAACAACGAGAGGCCGGGAAAGGAAGTTCGCCGAATCAACACGAGGCTGCCGGAGATGACCGAGGTGGTCAAGATGAAGGAGTTGATACGGAAAAAATGACTCGCGAGATTGGTGGACCAAAAGGCCCCGAGCCCACCCGCTACGGCGATTGGGAACGGAATGGTCGCTTGTTGAACAAAATTTCGGAGAAATTAGAGGAAGGAGATTAG

Protein sequence

MSSNLSRLFSSPPTLSALPALSRLTFNSLSRFVSSSSNQQPSRLEQGVSSEEQREAGKGSSPNQHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERNGRLLNKISEKLEEGD*
Homology
[Close]
BLAST of Chy9G171480 vs. ExPASy Swiss-Prot
Match: Q8BTE0 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Mus musculus OX=10090 GN=Sdhaf4 PE=3 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 3.2e-06
Identity = 21/31 (67.74%), Postives = 25/31 (80.65%), Query Frame = 0 

Query: 80  DTEKMTREIGGPKGPEPTRYGDWERNGRLLN 111
           D   +T+E GGPKGPEPTRYGDWER GR ++
Sbjct: 73  DVNPVTKEKGGPKGPEPTRYGDWERKGRCID 103

[Close]
BLAST of Chy9G171480 vs. ExPASy Swiss-Prot
Match: Q5VUM1 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Homo sapiens OX=9606 GN=SDHAF4 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 7.2e-06
Identity = 20/31 (64.52%), Postives = 25/31 (80.65%), Query Frame = 0 

Query: 80  DTEKMTREIGGPKGPEPTRYGDWERNGRLLN 111
           D   +T+E GGP+GPEPTRYGDWER GR ++
Sbjct: 77  DVNPVTKEKGGPRGPEPTRYGDWERKGRCID 107

[Close]
BLAST of Chy9G171480 vs. ExPASy Swiss-Prot
Match: Q9VLU6 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Drosophila melanogaster OX=7227 GN=Sirup PE=3 SV=2)

HSP 1 Score: 50.1 bits (118), Expect = 2.1e-05
Identity = 21/28 (75.00%), Postives = 22/28 (78.57%), Query Frame = 0 

Query: 81  TEKMTREIGGPKGPEPTRYGDWERNGRL 109
           T   T EIGGP GPEPTRYGDWER GR+
Sbjct: 88  TNPYTGEIGGPAGPEPTRYGDWERKGRV 115

[Close]
BLAST of Chy9G171480 vs. ExPASy TrEMBL
Match: A0A0A0KQ66 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucumis sativus OX=3659 GN=Csa_5G139490 PE=3 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.9e-46
Identity = 103/107 (96.26%), Postives = 106/107 (99.07%), Query Frame = 0 

Query: 1   MSSNLSRLFSSPPTLSALPALSRLTFNSLSRFVSSSSNQQPSRLEQGVSSEEQREAGKGS 60
           MSSNLSRLFSSPPTLSALPA+SRLTFNSLSRFVSSSSNQQPSRLE+GVSSEEQREAGKGS
Sbjct: 1   MSSNLSRLFSSPPTLSALPAVSRLTFNSLSRFVSSSSNQQPSRLEKGVSSEEQREAGKGS 60

Query: 61  SPNQHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERNGR 108
           SPN+HEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWER GR
Sbjct: 61  SPNRHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERKGR 107

[Close]
BLAST of Chy9G171480 vs. ExPASy TrEMBL
Match: A0A5A7TMD3 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00530 PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.6e-40
Identity = 95/107 (88.79%), Postives = 98/107 (91.59%), Query Frame = 0 

Query: 1   MSSNLSRLFSSPPTLSALPALSRLTFNSLSRFVSSSSNQQPSRLEQGVSSEEQREAGKGS 60
           MSS+LSRLFSSPPTLSALP + RLT NSLSRFVSSSSNQQPSR EQGVSSEEQREA KGS
Sbjct: 1   MSSHLSRLFSSPPTLSALPTVGRLTSNSLSRFVSSSSNQQPSRSEQGVSSEEQREAEKGS 60

Query: 61  SPNQHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERNGR 108
           SPNQHEAAGDDRG QD+GVDTEKM  EIGGPKG EPTRYGDWERNGR
Sbjct: 61  SPNQHEAAGDDRGSQDQGVDTEKMAPEIGGPKGHEPTRYGDWERNGR 107

[Close]
BLAST of Chy9G171480 vs. ExPASy TrEMBL
Match: A0A6J1K722 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111491251 PE=3 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 4.2e-33
Identity = 81/107 (75.70%), Postives = 92/107 (85.98%), Query Frame = 0 

Query: 1   MSSNLSRLFSSPPTLSALPALSRLTFNSLSRFVSSSSNQQPSRLEQGVSSEEQREAGKGS 60
           MSSNL+RLF SPP+L+AL A++R+T  S+SR VSSS+NQQPSRLEQGV  EEQREAGKGS
Sbjct: 1   MSSNLTRLF-SPPSLTALSAVNRVTSTSVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  SPNQHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERNGR 108
            P+QHEAAGDDR  +DEG D  K T E+GGPKGPEPTRYGDWERNGR
Sbjct: 61  LPDQHEAAGDDRDSEDEGADVNKATGEVGGPKGPEPTRYGDWERNGR 106

[Close]
BLAST of Chy9G171480 vs. ExPASy TrEMBL
Match: A0A6J1H1T7 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111459345 PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.6e-32
Identity = 81/107 (75.70%), Postives = 91/107 (85.05%), Query Frame = 0 

Query: 1   MSSNLSRLFSSPPTLSALPALSRLTFNSLSRFVSSSSNQQPSRLEQGVSSEEQREAGKGS 60
           MSSNL+RLF SPP+L+AL A++R T  S+SR VSSS+NQQPSRLEQGV  EEQREAGKGS
Sbjct: 1   MSSNLTRLF-SPPSLTALSAVNRATSISVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  SPNQHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERNGR 108
            P+QHEAAGDDR  +DEG D  K T E+GGPKGPEPTRYGDWERNGR
Sbjct: 61  LPDQHEAAGDDRDSEDEGADVNKATGEVGGPKGPEPTRYGDWERNGR 106

[Close]
BLAST of Chy9G171480 vs. ExPASy TrEMBL
Match: A0A540K2K1 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Malus baccata OX=106549 GN=C1H46_021803 PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.1e-09
Identity = 52/109 (47.71%), Postives = 67/109 (61.47%), Query Frame = 0 

Query: 1   MSSNLSRLFSSP--PTLSALPALSRLTFNSLSRFVSSSSNQQPSRLEQGVSSEEQREAGK 60
           M+ NL RLFSS   PT+SA   L+R   NS+SR + SS++Q  ++ E+   S+EQ E   
Sbjct: 1   MAINLGRLFSSKTIPTISAAEPLTRAGVNSVSRVLCSSASQSQTQHEK--PSKEQGEPIV 60

Query: 61  GSSPNQHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERNGR 108
               NQ E   DD G  ++     K T EIGGP+GPEPTR+GDWER GR
Sbjct: 61  EKLENQAEEEEDDDGDDNDDGFVNKKTGEIGGPRGPEPTRFGDWERTGR 107

[Close]
BLAST of Chy9G171480 vs. NCBI nr
Match: KGN49871.1 (hypothetical protein Csa_000062 [Cucumis sativus])

HSP 1 Score: 195 bits (495), Expect = 4.64e-62
Identity = 103/107 (96.26%), Postives = 106/107 (99.07%), Query Frame = 0 

Query: 1   MSSNLSRLFSSPPTLSALPALSRLTFNSLSRFVSSSSNQQPSRLEQGVSSEEQREAGKGS 60
           MSSNLSRLFSSPPTLSALPA+SRLTFNSLSRFVSSSSNQQPSRLE+GVSSEEQREAGKGS
Sbjct: 1   MSSNLSRLFSSPPTLSALPAVSRLTFNSLSRFVSSSSNQQPSRLEKGVSSEEQREAGKGS 60

Query: 61  SPNQHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERNGR 107
           SPN+HEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWER GR
Sbjct: 61  SPNRHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERKGR 107

[Close]
BLAST of Chy9G171480 vs. NCBI nr
Match: KAA0042555.1 (succinate dehydrogenase assembly factor 4 [Cucumis melo var. makuwa] >TYK05959.1 succinate dehydrogenase assembly factor 4 [Cucumis melo var. makuwa])

HSP 1 Score: 176 bits (445), Expect = 1.95e-54
Identity = 95/110 (86.36%), Postives = 99/110 (90.00%), Query Frame = 0 

Query: 1   MSSNLSRLFSSPPTLSALPALSRLTFNSLSRFVSSSSNQQPSRLEQGVSSEEQREAGKGS 60
           MSS+LSRLFSSPPTLSALP + RLT NSLSRFVSSSSNQQPSR EQGVSSEEQREA KGS
Sbjct: 1   MSSHLSRLFSSPPTLSALPTVGRLTSNSLSRFVSSSSNQQPSRSEQGVSSEEQREAEKGS 60

Query: 61  SPNQHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERNGRLLN 110
           SPNQHEAAGDDRG QD+GVDTEKM  EIGGPKG EPTRYGDWERNGR  +
Sbjct: 61  SPNQHEAAGDDRGSQDQGVDTEKMAPEIGGPKGHEPTRYGDWERNGRCYD 110

[Close]
BLAST of Chy9G171480 vs. NCBI nr
Match: XP_022995844.1 (succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita maxima])

HSP 1 Score: 151 bits (381), Expect = 1.06e-44
Identity = 81/110 (73.64%), Postives = 93/110 (84.55%), Query Frame = 0 

Query: 1   MSSNLSRLFSSPPTLSALPALSRLTFNSLSRFVSSSSNQQPSRLEQGVSSEEQREAGKGS 60
           MSSNL+RLFS PP+L+AL A++R+T  S+SR VSSS+NQQPSRLEQGV  EEQREAGKGS
Sbjct: 1   MSSNLTRLFS-PPSLTALSAVNRVTSTSVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  SPNQHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERNGRLLN 110
            P+QHEAAGDDR  +DEG D  K T E+GGPKGPEPTRYGDWERNGR  +
Sbjct: 61  LPDQHEAAGDDRDSEDEGADVNKATGEVGGPKGPEPTRYGDWERNGRCYD 109

[Close]
BLAST of Chy9G171480 vs. NCBI nr
Match: XP_022957978.1 (succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita moschata])

HSP 1 Score: 149 bits (376), Expect = 6.14e-44
Identity = 81/110 (73.64%), Postives = 92/110 (83.64%), Query Frame = 0 

Query: 1   MSSNLSRLFSSPPTLSALPALSRLTFNSLSRFVSSSSNQQPSRLEQGVSSEEQREAGKGS 60
           MSSNL+RLFS PP+L+AL A++R T  S+SR VSSS+NQQPSRLEQGV  EEQREAGKGS
Sbjct: 1   MSSNLTRLFS-PPSLTALSAVNRATSISVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  SPNQHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERNGRLLN 110
            P+QHEAAGDDR  +DEG D  K T E+GGPKGPEPTRYGDWERNGR  +
Sbjct: 61  LPDQHEAAGDDRDSEDEGADVNKATGEVGGPKGPEPTRYGDWERNGRCYD 109

[Close]
BLAST of Chy9G171480 vs. NCBI nr
Match: XP_023532848.1 (succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 148 bits (373), Expect = 1.76e-43
Identity = 80/110 (72.73%), Postives = 92/110 (83.64%), Query Frame = 0 

Query: 1   MSSNLSRLFSSPPTLSALPALSRLTFNSLSRFVSSSSNQQPSRLEQGVSSEEQREAGKGS 60
           MSSNL+RLFS PP+L++L A++R T  S+SR VSSS+NQQPSRLEQGV  EEQREAGKGS
Sbjct: 1   MSSNLTRLFS-PPSLTSLSAVNRATSISVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  SPNQHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERNGRLLN 110
            P+QHEAAGDDR  +DEG D  K T E+GGPKGPEPTRYGDWERNGR  +
Sbjct: 61  LPDQHEAAGDDRDSEDEGADVNKATGEVGGPKGPEPTRYGDWERNGRCYD 109

[Close]
BLAST of Chy9G171480 vs. TAIR 10
Match: AT5G67490.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1674 (InterPro:IPR012875); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 41.6 bits (96), Expect = 5.3e-04
Identity = 31/85 (36.47%), Postives = 42/85 (49.41%), Query Frame = 0 

Query: 29  LSRFVSSSSNQQPSR------LEQGVSSEEQREAGKGSSPNQHEAAGDDRGGQDEGVDTE 88
           +SRF+SS +   P        L + V  ++  +        + E      GG+    D  
Sbjct: 24  VSRFLSSGTPPPPQAPSPNQDLNRDVKPDQNLQQNLQMQKEEEEEGEGGGGGEFVNED-- 83

Query: 89  KMTREIGGPKGPEPTRYGDWERNGR 108
             T EIGGP+GPEPTRYGDWE+ GR
Sbjct: 84  --TGEIGGPRGPEPTRYGDWEQRGR 104

The following BLAST results are available for this feature:
BLAST of Chy9G171480 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8BTE03.2e-0667.74Succinate dehydrogenase assembly factor 4, mitochondrial OS=Mus musculus OX=1009... [more]
Q5VUM17.2e-0664.52Succinate dehydrogenase assembly factor 4, mitochondrial OS=Homo sapiens OX=9606... [more]
Q9VLU62.1e-0575.00Succinate dehydrogenase assembly factor 4, mitochondrial OS=Drosophila melanogas... [more]
BLAST of Chy9G171480 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KQ661.9e-4696.26Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucumis sativus OX=3... [more]
A0A5A7TMD31.6e-4088.79Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucumis melo var. ma... [more]
A0A6J1K7224.2e-3375.70Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucurbita maxima OX=... [more]
A0A6J1H1T71.6e-3275.70Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucurbita moschata O... [more]
A0A540K2K11.1e-0947.71Succinate dehydrogenase assembly factor 4, mitochondrial OS=Malus baccata OX=106... [more]
BLAST of Chy9G171480 vs. NCBI nr
Match NameE-valueIdentityDescription
KGN49871.14.64e-6296.26hypothetical protein Csa_000062 [Cucumis sativus][more]
KAA0042555.11.95e-5486.36succinate dehydrogenase assembly factor 4 [Cucumis melo var. makuwa] >TYK05959.1... [more]
XP_022995844.11.06e-4473.64succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita maxima][more]
XP_022957978.16.14e-4473.64succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita moschata][more]
XP_023532848.11.76e-4372.73succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita pepo subsp. ... [more]
BLAST of Chy9G171480 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G67490.15.3e-0436.47unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1674... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012875Succinate dehydrogenase assembly factor 4PFAMPF07896DUF1674coord: 80..107
e-value: 5.8E-12
score: 45.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..120
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..89
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..120
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..57
NoneNo IPR availablePANTHERPTHR28524SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 4, MITOCHONDRIALcoord: 1..108

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy9G171480.1Chy9G171480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034553 mitochondrial respiratory chain complex II assembly
cellular_component GO:0005739 mitochondrion