Chy9G171040 (gene) Cucumber (hystrix) v1

Overview
NameChy9G171040
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionPectin lyase-like superfamily protein
LocationchrH09: 15623808 .. 15626165 (+)
RNA-Seq ExpressionChy9G171040
SyntenyChy9G171040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCTTCCTCAAAAGCCCACAAAAACTCATGTAATTTTCTTTTCTTTTTTAATGTCTCTTTCATTCTTTTTCGTTTTTCTGGTAATTTACATGTTGATTATTGAAAATGATTAGTGGGTTTTTCTTCAATTTCCTCGAGAACAATTATCTCGAGATCGTTCAAAAATGTATCCTATGAAAGATGAAAAAGAAAAAAAACCCAAGAATCGTTTCTTTTTCTCTGTTCGATTATCTCGAGAAACAAACACATATTGAATGATCAGTTTTGCTTTTTATGGCAGGATGCGGCGGCGAGAATCTCGGCCGTGATTTTACTCGCATTGGTGAGTTTAGCCACCGTGGAAGGGAAAAGGGCGTGCCCGACTAATTATTTACAAGTACCGGCGATCAGTTGCCGGAAACACACGGCGTTGATTACGGACTTCGGCGGCGTAGGCGACGGTGTAACTTCAAATACTCAGGCGTTCCGGCGAGCGATTGAACATCTCAGCTCAATGGCAGCGGACGGCGGTGCTCAGTTGATTGTGCCACCGGGAAAATGGCTGACCGGAAGTTTTAATCTGACCAGCCATTTCACACTCTTTGTTCATAAAGATGCAAAAATTCTTGCTTCCCAGGTTTGACCTTCAAACAATATACATATCTATATATGGGAATGCATCTGTTCTAGTTTATATGTATATATAGAAATGTAATCTGTCTGATAATCCTTATGTATTTTGTAGTTTTAGTATTTTTTTGGATTAATTTGATGTTAAATATATACTATTTTTCTATTAAAAGAAAAGCATATGAATCTTATTATATAATTTGGATTCAAAAAGGTGATTTTGGAACAAACAGAAAGTGGAAATGTTCCAATGGCAAGAAAGTCAAGTTTAGTGTAGTACAAAACAGATGGTTTGTAGTGTTTGTTTTTTACTGAATTTTTCAAAGAATAAATTTGGTAATACATTATTCTAAGACTACATACTTACGTGTCTAAATCTTTTTGGGACTTTTTCTTTGAAATTAGGTCTGAGTCATGGAGGATCCATATATTCTTAAAATTGAAATTCTAATAAAAATAAAAGGGGTAGGAATTTATGGACTTTGATTGTTGGAGTCAAATAGAAAATGAGTATTGATTAAATCATGGGGGTTGGTGATTTGTTGGGGGAACTAATTGAGTTTGCATAATCCAAAGGTGGAGAATGAATCGGAATGGCCACAAGTCGCAATTCTACCATCTTATGGGATAGGAAGAGATGCTCCTGGCGGAAGATACAGCAGTCTCATCTTTGGAACCAATCTCACTGATGTCGTCATCACCGGTAACAATGGTACCATTGACGGGCAAGGAGCTTACTGGTGGGATAAGTTCCACAAAGGCGAGCTTAACTTGACACGGCCCTACCTAATTGAGATTATGTACTCCGATCAAATTCAGATTTCTGATCTAACTTTGGTCAATTCTCCCTCCTGGTTTGTCCATCCCATCTACAGTAAGAATGTGATTATCCAAGGTCTCACTATTCTTGCTCCCATCGACTCTCCCAACACCGACGGAATCGACCCAGGTTTCCGTCTTCAGACCCTTCAAATGGGTTATGGCTTTTCTTCTTCATCTTCCCTCTCCCTAATAATTCTTCTTTTTGTTATTTCACAGATTCCTGCTCCAACACTCGTATCGAAGACTGCTTCATTGTCTCCGGCGACGACTGCATTGCTGTCAAGAGCGGATGGGACCAGTACGGTATCAAATTCGGAATGCCAACTGAAGACCTCGTCATTAAACGCCTCACCTGCATTTCCCCGGATTCTGCTGGCATCGCTCTCGGCAGTGAAATGTCCGGCGGTATCCGCAACGTCAGAATCGAAAACGTCACCGGAATCAATACACAATCCGCTGTGAGAATCAAAACCGCTCGTGGACGTGGGGGTTTCGTGAAGGATATTTTCGTCCGTGGAATGTATCTATCGACGATGAAATACGTGTTCTGGATGACCGGAAACTACAAATCGCATCCTGATGACAAGTTCGACCCCGCGGCGTTGCCGGAGATTACGAACATTAATTACAGAGATGTGGTGGCGGAAAACGTTACTTACTCGGCGAGATTGGAAGGGATTTCGGGAGATCCGTTCACCAATATTTGTATTTCAAATGTTAAGATTGGACTGACGGCGAAGCCTAAAAAGTTGCAGTGGAACTGTACAGATGTTGAGGGATTCAGTAGCGATGTGGTGCCGCCGCCTTGTGCTCCTCTGGCGAAGGCGGGGAAAAGCGGCGGATGTGACTTTCCTGAGGATCAACTTCCGATTGAGAAGGTTCAGTTCAAGACTTGCTTTGTTCAAAATCTCACCGCCGCGTGA

mRNA sequence

ATGGATCTTCCTCAAAAGCCCACAAAAACTCATGATGCGGCGGCGAGAATCTCGGCCGTGATTTTACTCGCATTGGTGAGTTTAGCCACCGTGGAAGGGAAAAGGGCGTGCCCGACTAATTATTTACAAGTACCGGCGATCAGTTGCCGGAAACACACGGCGTTGATTACGGACTTCGGCGGCGTAGGCGACGGTGTAACTTCAAATACTCAGGCGTTCCGGCGAGCGATTGAACATCTCAGCTCAATGGCAGCGGACGGCGGTGCTCAGTTGATTGTGCCACCGGGAAAATGGCTGACCGGAAGTTTTAATCTGACCAGCCATTTCACACTCTTTGTTCATAAAGATGCAAAAATTCTTGCTTCCCAGGTGGAGAATGAATCGGAATGGCCACAAGTCGCAATTCTACCATCTTATGGGATAGGAAGAGATGCTCCTGGCGGAAGATACAGCAGTCTCATCTTTGGAACCAATCTCACTGATGTCGTCATCACCGGTAACAATGGTACCATTGACGGGCAAGGAGCTTACTGGTGGGATAAGTTCCACAAAGGCGAGCTTAACTTGACACGGCCCTACCTAATTGAGATTATGTACTCCGATCAAATTCAGATTTCTGATCTAACTTTGGTCAATTCTCCCTCCTGGTTTGTCCATCCCATCTACAGTAAGAATGTGATTATCCAAGGTCTCACTATTCTTGCTCCCATCGACTCTCCCAACACCGACGGAATCGACCCAGATTCCTGCTCCAACACTCGTATCGAAGACTGCTTCATTGTCTCCGGCGACGACTGCATTGCTGTCAAGAGCGGATGGGACCAGTACGGTATCAAATTCGGAATGCCAACTGAAGACCTCGTCATTAAACGCCTCACCTGCATTTCCCCGGATTCTGCTGGCATCGCTCTCGGCAGTGAAATGTCCGGCGGTATCCGCAACGTCAGAATCGAAAACGTCACCGGAATCAATACACAATCCGCTGTGAGAATCAAAACCGCTCGTGGACGTGGGGGTTTCGTGAAGGATATTTTCGTCCGTGGAATGTATCTATCGACGATGAAATACGTGTTCTGGATGACCGGAAACTACAAATCGCATCCTGATGACAAGTTCGACCCCGCGGCGTTGCCGGAGATTACGAACATTAATTACAGAGATGTGGTGGCGGAAAACGTTACTTACTCGGCGAGATTGGAAGGGATTTCGGGAGATCCGTTCACCAATATTTGTATTTCAAATGTTAAGATTGGACTGACGGCGAAGCCTAAAAAGTTGCAGTGGAACTGTACAGATGTTGAGGGATTCAGTAGCGATGTGGTGCCGCCGCCTTGTGCTCCTCTGGCGAAGGCGGGGAAAAGCGGCGGATGTGACTTTCCTGAGGATCAACTTCCGATTGAGAAGGTTCAGTTCAAGACTTGCTTTGTTCAAAATCTCACCGCCGCGTGA

Coding sequence (CDS)

ATGGATCTTCCTCAAAAGCCCACAAAAACTCATGATGCGGCGGCGAGAATCTCGGCCGTGATTTTACTCGCATTGGTGAGTTTAGCCACCGTGGAAGGGAAAAGGGCGTGCCCGACTAATTATTTACAAGTACCGGCGATCAGTTGCCGGAAACACACGGCGTTGATTACGGACTTCGGCGGCGTAGGCGACGGTGTAACTTCAAATACTCAGGCGTTCCGGCGAGCGATTGAACATCTCAGCTCAATGGCAGCGGACGGCGGTGCTCAGTTGATTGTGCCACCGGGAAAATGGCTGACCGGAAGTTTTAATCTGACCAGCCATTTCACACTCTTTGTTCATAAAGATGCAAAAATTCTTGCTTCCCAGGTGGAGAATGAATCGGAATGGCCACAAGTCGCAATTCTACCATCTTATGGGATAGGAAGAGATGCTCCTGGCGGAAGATACAGCAGTCTCATCTTTGGAACCAATCTCACTGATGTCGTCATCACCGGTAACAATGGTACCATTGACGGGCAAGGAGCTTACTGGTGGGATAAGTTCCACAAAGGCGAGCTTAACTTGACACGGCCCTACCTAATTGAGATTATGTACTCCGATCAAATTCAGATTTCTGATCTAACTTTGGTCAATTCTCCCTCCTGGTTTGTCCATCCCATCTACAGTAAGAATGTGATTATCCAAGGTCTCACTATTCTTGCTCCCATCGACTCTCCCAACACCGACGGAATCGACCCAGATTCCTGCTCCAACACTCGTATCGAAGACTGCTTCATTGTCTCCGGCGACGACTGCATTGCTGTCAAGAGCGGATGGGACCAGTACGGTATCAAATTCGGAATGCCAACTGAAGACCTCGTCATTAAACGCCTCACCTGCATTTCCCCGGATTCTGCTGGCATCGCTCTCGGCAGTGAAATGTCCGGCGGTATCCGCAACGTCAGAATCGAAAACGTCACCGGAATCAATACACAATCCGCTGTGAGAATCAAAACCGCTCGTGGACGTGGGGGTTTCGTGAAGGATATTTTCGTCCGTGGAATGTATCTATCGACGATGAAATACGTGTTCTGGATGACCGGAAACTACAAATCGCATCCTGATGACAAGTTCGACCCCGCGGCGTTGCCGGAGATTACGAACATTAATTACAGAGATGTGGTGGCGGAAAACGTTACTTACTCGGCGAGATTGGAAGGGATTTCGGGAGATCCGTTCACCAATATTTGTATTTCAAATGTTAAGATTGGACTGACGGCGAAGCCTAAAAAGTTGCAGTGGAACTGTACAGATGTTGAGGGATTCAGTAGCGATGTGGTGCCGCCGCCTTGTGCTCCTCTGGCGAAGGCGGGGAAAAGCGGCGGATGTGACTTTCCTGAGGATCAACTTCCGATTGAGAAGGTTCAGTTCAAGACTTGCTTTGTTCAAAATCTCACCGCCGCGTGA

Protein sequence

MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRACPTNYLQVPAISCRKHTALITDFGGVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQVENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWDKFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSAGIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGFSSDVVPPPCAPLAKAGKSGGCDFPEDQLPIEKVQFKTCFVQNLTAA*
Homology
BLAST of Chy9G171040 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 7.5e-179
Identity = 295/457 (64.55%), Postives = 363/457 (79.43%), Query Frame = 0

Query: 20  VILLALVSLATVEGKRACPTNYLQVPAISCRKHTALITDFGGVGDGVTSNTQAFRRAIEH 79
           V ++ L+S++  E ++      L+  AISCR H+A + DFGGVGDG T NT+AF+ A+  
Sbjct: 29  VAVVLLLSVSRGECRKGRILEALEYSAISCRAHSASLVDFGGVGDGQTLNTKAFQDAVSE 88

Query: 80  LSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQVENESEWPQVAILPSY 139
           LS   ++GGAQL VP GKWLTGSF+LTSHFTLF+H+DA +LASQ  + S+WP +  LPSY
Sbjct: 89  LSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLHRDAVLLASQ--DISQWPVIKPLPSY 148

Query: 140 GIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWDKFHKGELNLTRPYLIEIMY 199
           G GRDA  GRY+SLIFGTNLTDV+ITG+NGTIDGQG  WW +FH G+L  TRPYLIE+MY
Sbjct: 149 GRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGGLWWQRFHGGKLKYTRPYLIELMY 208

Query: 200 SDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIEDCF 259
           S  IQIS+LTL+NSPSW VHP+YS+N++IQG+TILAP+ SPNTDGI+PDSC+NTRIEDC+
Sbjct: 209 SADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAPVRSPNTDGINPDSCTNTRIEDCY 268

Query: 260 IVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSAGIALGSEMSGGIRNVRIEN 319
           IVSGDDC+AVKSGWD+YGI +GMPT+ LVI+RLTCISP SA IALGSEMSGGI++VR E+
Sbjct: 269 IVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCISPYSAVIALGSEMSGGIQDVRAED 328

Query: 320 VTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPAALPE 379
           +  IN++S +RIKT  GRGG+VKDI+VRGM + TMK+ FWMTGNY SH D+ +DP A P 
Sbjct: 329 IVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKWAFWMTGNYGSHADNHYDPKAFPV 388

Query: 380 ITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGFSSD 439
           I  INYRD+VAENV+ +ARLEGI  DPFT ICISNV I L AK KK+ W CTDVEG SS 
Sbjct: 389 IQGINYRDMVAENVSMAARLEGIPSDPFTGICISNVTIHLAAKAKKVPWTCTDVEGISSG 448

Query: 440 VVPPPCAPLAKAG--KSGGCDFPEDQLPIEKVQFKTC 475
           V P PC+ L   G  K+  C+FP + LPI+ V+ + C
Sbjct: 449 VTPTPCSTLPDQGPEKTSLCNFPAESLPIDTVELQKC 483

BLAST of Chy9G171040 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 5.0e-34
Identity = 121/403 (30.02%), Postives = 185/403 (45.91%), Query Frame = 0

Query: 56  ITDFGGVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGKW-------LTGSFNLTSH 115
           +T FG VGDGVT ++QAF +A E + S   DG  Q +VP G           GS   T  
Sbjct: 26  VTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDG--QFVVPAGMTFMLQPLKFQGSCKST-- 85

Query: 116 FTLFVHKDAKILASQVENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVITGNN 175
             +FV    K++A               PS G  +    G     I  T++  +VI G +
Sbjct: 86  -PVFVQMLGKLVA---------------PSKGNWK----GDKDQWILFTDIEGLVIEG-D 145

Query: 176 GTIDGQGAYWWDKFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVII 235
           G I+GQG+ WW+  HKG    +RP  ++    + +++S LT ++SP   +H      V I
Sbjct: 146 GEINGQGSSWWE--HKG----SRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTI 205

Query: 236 QGLTILAPIDSPNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLV 295
             L I AP  SPNTDGID  + SN  I+DC I +GDDCIA+ SG           T ++ 
Sbjct: 206 SSLRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TSNIH 265

Query: 296 IKRLTCISPDSAGIALGSEMSGG----IRNVRIENVTGINTQSAVRIKTARGRGGFVKDI 355
           I  + C      GI++GS    G    + NV ++N     T +  RIKT +G  G+ + I
Sbjct: 266 ISGIDC--GPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMI 325

Query: 356 FVRGMYLSTMKYVFWMTGNYKSHPDD--KFDPAALPEITNINYRDVVAENVT-YSARLEG 415
              G+ L  ++    +   Y     D  K   ++  E++ + + + +  + + Y      
Sbjct: 326 TFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRC 384

Query: 416 ISGDPFTNICISNVKI--GLTAKPKKLQWNCTDVEGFSSDVVP 443
               P T I + ++KI    +   +  Q  C +V G S+  VP
Sbjct: 386 SERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVP 384

BLAST of Chy9G171040 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.2e-28
Identity = 117/409 (28.61%), Postives = 176/409 (43.03%), Query Frame = 0

Query: 56  ITDFGGVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHK 115
           +  +G VGDG   +T+AF       ++      A L+VP  K    + NL        H 
Sbjct: 61  VEQYGAVGDGKHDSTEAFATT---WNAACKKASAVLLVPANKKFFVN-NLVFRGPCQPHL 120

Query: 116 DAKILASQV--ENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDG 175
             K+  + V   + + W    I   +                   LTD  + G  G IDG
Sbjct: 121 SFKVDGTIVAQPDPARWKNSKIWLQF-----------------AQLTDFNLMG-TGVIDG 180

Query: 176 QGAYWWD---KFHKGEL---NLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVI 235
           QG  WW    K   G     +  RP  I+I YS  + + +LTL+NSP + +     + V 
Sbjct: 181 QGQQWWAGQCKVVNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVK 240

Query: 236 IQGLTILAPIDSPNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDL 295
           IQGL I AP DSPNTDGID  +     IE C I +GDDCIA+ +G           + ++
Sbjct: 241 IQGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTG-----------SSNI 300

Query: 296 VIKRLTCISPDSAGIALGS----EMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKD 355
            IK L C      GI++GS         + +V +     I+TQ+ +RIKT +G  G    
Sbjct: 301 TIKDLIC--GPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASY 360

Query: 356 IFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSA-RLEGI 415
           I    + +   +    +   Y +      +  +  +I  + Y+++   + T +A +L   
Sbjct: 361 ITYENVEMINSENPILINQFYCTSASACQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCS 420

Query: 416 SGDPFTNICISNVKIGLTA-KPKKLQWNCTD--VEGFSSDVVPPPCAPL 449
              P T I +SNV + LT+ KP     +C D    GF S  + P C  L
Sbjct: 421 DSVPCTGIQLSNVSLKLTSGKPA----SCVDKNARGFYSGRLIPTCKNL 430

BLAST of Chy9G171040 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 1.0e-26
Identity = 110/408 (26.96%), Postives = 179/408 (43.87%), Query Frame = 0

Query: 56  ITDFGGVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHK 115
           +  +G VGDG   +T AF +      +  +   A  +VP  K    + NL  +     H 
Sbjct: 62  VEHYGAVGDGKHDSTDAFEKTWNAACNKLS---AVFLVPANKKFVVN-NLVFYGPCQPHF 121

Query: 116 DAKI--LASQVENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDG 175
             K+    +   + ++W    I   +                   LTD  + G  G IDG
Sbjct: 122 SFKVDGTIAAYPDPAKWKNSKIWMHF-----------------ARLTDFNLMG-TGVIDG 181

Query: 176 QGAYWWDKFHKGELNLT------RPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSK--N 235
           QG  WW    K     T      RP  I+I +S  + + +LTL NSP +  H ++ +   
Sbjct: 182 QGNRWWSDQCKTINGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEF--HLVFGECDG 241

Query: 236 VIIQGLTILAPIDSPNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTE 295
           V IQG+ I AP DSPNTDGID  +     IE C I +GDDC+AV +G           + 
Sbjct: 242 VKIQGIKIKAPRDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTG-----------SS 301

Query: 296 DLVIKRLTCISPDSAGIALGSEMSGGIRN----VRIENVTGINTQSAVRIKTARGRGGFV 355
           ++ IK LTC      G+++GS   G  R+    V ++    I+TQ+ +RIKT +G  G  
Sbjct: 302 NITIKDLTC--GPGHGMSIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLA 361

Query: 356 KDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSA-RLE 415
             I    + +   +    +   Y +      +  +  +I ++ ++++   + T +A +L 
Sbjct: 362 SHITYENVEMINAENPILINQFYCTSAAACKNQRSAVKIQDVTFKNIHGTSATTAAIQLM 421

Query: 416 GISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGFSSDVVPPPCAPL 449
                P +NI +SNV + LT+       N  +  G+ ++ + P C  L
Sbjct: 422 CSDSVPCSNIKLSNVFLKLTSGKVATCVN-KNANGYYTNPLNPSCKSL 431

BLAST of Chy9G171040 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 117.5 bits (293), Expect = 4.3e-25
Identity = 100/408 (24.51%), Postives = 169/408 (41.42%), Query Frame = 0

Query: 53  TALITDFGGVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGK-------WLTGSFNL 112
           T  ++DFG  GDG T +TQAF  A +   + +++G   L+VP G         LTG  N 
Sbjct: 67  TVSVSDFGAKGDGKTDDTQAFVNAWK--KACSSNGAVNLLVPKGNTYLLKSIQLTGPCNS 126

Query: 113 TSHFTLFVHKDAKILASQVENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVIT 172
                +F    A    S  ++ S+W                      ++F       V  
Sbjct: 127 ILTVQIFGTLSASQKRSDYKDISKW----------------------IMFDGVNNLSVDG 186

Query: 173 GNNGTIDGQGAYWWD---KFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIY 232
           G+ G +DG G  WW    K +K +     P  +    S  + + +L + N+    +    
Sbjct: 187 GDTGVVDGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEK 246

Query: 233 SKNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGM 292
             NV +  + + AP DSPNTDGI   +  N R+ +  I +GDDCI+++SG          
Sbjct: 247 CSNVQVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG---------- 306

Query: 293 PTEDLVIKRLTCISPDSAGIALGS----EMSGGIRNVRIENVTGINTQSAVRIKTARGRG 352
            ++++ I  +TC      GI++GS         +  V ++      T + VRIKT +G  
Sbjct: 307 -SQNVQINDITC--GPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGS 366

Query: 353 GFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSAR 412
           G   +I  + + +  +K    +  +Y        + +A+ ++ N+ YRD+   + + +A 
Sbjct: 367 GTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAV-QVKNVVYRDISGTSASENAI 426

Query: 413 LEGISGD-PFTNICISNVKIGLTAKPKKLQWNCTDVEGFSSDVVPPPC 446
               S + P   I +  V I      K  +  CT+        V P C
Sbjct: 427 TFNCSKNYPCQGIVLDRVNI------KGGKATCTNANVVDKGAVLPQC 430

BLAST of Chy9G171040 vs. ExPASy TrEMBL
Match: A0A0A0KNF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G140480 PE=3 SV=1)

HSP 1 Score: 969.5 bits (2505), Expect = 5.0e-279
Identity = 465/480 (96.88%), Postives = 472/480 (98.33%), Query Frame = 0

Query: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRACPTNYLQVPAISCRKHTALITDFG 60
           M LPQ PTKTHDAAARISAVILLAL+SLATVEGKR+CPTNYLQVPAISCRKHTALITDFG
Sbjct: 1   MYLPQDPTKTHDAAARISAVILLALLSLATVEGKRSCPTNYLQVPAISCRKHTALITDFG 60

Query: 61  GVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKIL 120
           GVGDGVTSNTQAFRRAIEHLSS+AADGGAQL VPPGKWLTGSFNLTSHFTLFVHKDAKIL
Sbjct: 61  GVGDGVTSNTQAFRRAIEHLSSLAADGGAQLTVPPGKWLTGSFNLTSHFTLFVHKDAKIL 120

Query: 121 ASQVENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWD 180
            SQVENESEWPQVA+LPSYG+GRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWD
Sbjct: 121 GSQVENESEWPQVALLPSYGVGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWD 180

Query: 181 KFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP 240
           KFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP
Sbjct: 181 KFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP 240

Query: 241 NTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSA 300
           NTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSA
Sbjct: 241 NTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSA 300

Query: 301 GIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM 360
           G+ALGSEMSGGIRNVRIEN TGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM
Sbjct: 301 GVALGSEMSGGIRNVRIENFTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM 360

Query: 361 TGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT 420
           TGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT
Sbjct: 361 TGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT 420

Query: 421 AKPKKLQWNCTDVEGFSSDVVPPPCAPLAKAGKSGGCDFPEDQLPIEKVQFKTCFVQNLT 480
           A PKKLQWNCTDVEGFSSDVVPPPCAPLAKAGKSGGCDFPEDQLPIE+VQ KTCFVQN T
Sbjct: 421 ATPKKLQWNCTDVEGFSSDVVPPPCAPLAKAGKSGGCDFPEDQLPIERVQLKTCFVQNPT 480

BLAST of Chy9G171040 vs. ExPASy TrEMBL
Match: A0A5D3C461 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00950 PE=3 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 4.6e-272
Identity = 454/480 (94.58%), Postives = 469/480 (97.71%), Query Frame = 0

Query: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRACPTNYLQVPAISCRKHTALITDFG 60
           MDLPQKPTKTHDAAARISAVILLALVSLATVEGKR CPTNYLQVPAI+CR+HTA++TDFG
Sbjct: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRQCPTNYLQVPAINCRQHTAVLTDFG 60

Query: 61  GVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKIL 120
           GVGDGVTSNTQAFRRAIEHLS +AA+GGAQLIVPPGKWLTGSFNLTSHFTLFVHKDA IL
Sbjct: 61  GVGDGVTSNTQAFRRAIEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATIL 120

Query: 121 ASQVENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWD 180
           ASQ  +ESEWPQVAILPSYG+GRDAPGGRYSSLI+GTNLTDVVITGNNGTIDGQG+YWWD
Sbjct: 121 ASQ--DESEWPQVAILPSYGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWD 180

Query: 181 KFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP 240
           KFHKGELNLTRPY+IEI+YSDQIQIS+LTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP
Sbjct: 181 KFHKGELNLTRPYMIEILYSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP 240

Query: 241 NTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSA 300
           NTDGIDPDSCSN RIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVI+RLTCISPDSA
Sbjct: 241 NTDGIDPDSCSNIRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSA 300

Query: 301 GIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM 360
           GIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM
Sbjct: 301 GIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM 360

Query: 361 TGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT 420
           TGNYKSHPD+KFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT
Sbjct: 361 TGNYKSHPDEKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT 420

Query: 421 AKPKKLQWNCTDVEGFSSDVVPPPCAPLAKAGKSGGCDFPEDQLPIEKVQFKTCFVQNLT 480
           AKPKKLQWNCTDVEG SSDVVPPPCAPLAKA KSGGCDFP DQLPIEKVQ KTCFVQN T
Sbjct: 421 AKPKKLQWNCTDVEGISSDVVPPPCAPLAKAAKSGGCDFPADQLPIEKVQLKTCFVQNPT 478

BLAST of Chy9G171040 vs. ExPASy TrEMBL
Match: A0A1S3AUV5 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482916 PE=3 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 4.6e-272
Identity = 454/480 (94.58%), Postives = 469/480 (97.71%), Query Frame = 0

Query: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRACPTNYLQVPAISCRKHTALITDFG 60
           MDLPQKPTKTHDAAARISAVILLALVSLATVEGKR CPTNYLQVPAI+CR+HTA++TDFG
Sbjct: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRQCPTNYLQVPAINCRQHTAVLTDFG 60

Query: 61  GVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKIL 120
           GVGDGVTSNTQAFRRAIEHLS +AA+GGAQLIVPPGKWLTGSFNLTSHFTLFVHKDA IL
Sbjct: 61  GVGDGVTSNTQAFRRAIEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATIL 120

Query: 121 ASQVENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWD 180
           ASQ  +ESEWPQVAILPSYG+GRDAPGGRYSSLI+GTNLTDVVITGNNGTIDGQG+YWWD
Sbjct: 121 ASQ--DESEWPQVAILPSYGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWD 180

Query: 181 KFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP 240
           KFHKGELNLTRPY+IEI+YSDQIQIS+LTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP
Sbjct: 181 KFHKGELNLTRPYMIEILYSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP 240

Query: 241 NTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSA 300
           NTDGIDPDSCSN RIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVI+RLTCISPDSA
Sbjct: 241 NTDGIDPDSCSNIRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSA 300

Query: 301 GIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM 360
           GIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM
Sbjct: 301 GIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM 360

Query: 361 TGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT 420
           TGNYKSHPD+KFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT
Sbjct: 361 TGNYKSHPDEKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT 420

Query: 421 AKPKKLQWNCTDVEGFSSDVVPPPCAPLAKAGKSGGCDFPEDQLPIEKVQFKTCFVQNLT 480
           AKPKKLQWNCTDVEG SSDVVPPPCAPLAKA KSGGCDFP DQLPIEKVQ KTCFVQN T
Sbjct: 421 AKPKKLQWNCTDVEGISSDVVPPPCAPLAKAAKSGGCDFPADQLPIEKVQLKTCFVQNPT 478

BLAST of Chy9G171040 vs. ExPASy TrEMBL
Match: A0A6J1K3G8 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111491343 PE=3 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 2.6e-243
Identity = 410/480 (85.42%), Postives = 442/480 (92.08%), Query Frame = 0

Query: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRACP-TNYLQVPAISCRKHTALITDF 60
           MDLP KPTKT   A RISAVILL LVSLA+V+GK+ CP ++YL+  AISCRKHTA++TDF
Sbjct: 1   MDLPHKPTKTQITAVRISAVILLVLVSLASVDGKKPCPFSSYLEFSAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKI 120
           GGVGDGVTSNT AFR AIEHLS++A DGG+QLIVPPGKWLTGSFNLTSHFTLFVHKDA+I
Sbjct: 61  GGVGDGVTSNTNAFREAIEHLSTLAIDGGSQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQVENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWW 180
           LASQ  +ESEWP V +LPSYG GRDAPGGR+SSLIFGTNLTDVVITGNNGTIDGQG+YWW
Sbjct: 121 LASQ--DESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWW 180

Query: 181 DKFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDS 240
           DKFHK E N+TRPY+IEIMYSDQIQIS+LTLVNSPSWFVHPIYS+NVIIQGLTILAPIDS
Sbjct: 181 DKFHKNEFNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDS 240

Query: 241 PNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDS 300
           PNTDGIDPDSC NTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTEDL+I+RLTCISPDS
Sbjct: 241 PNTDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDS 300

Query: 301 AGIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFW 360
           AGIALGSEMSGGIRNVRIENVT INTQSAVRIKTA GRGG+VKDIFVR M+LSTMKYVFW
Sbjct: 301 AGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFW 360

Query: 361 MTGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGL 420
           MTGNYKSHPD+KFDP ALPEI NINYRDVVAENVTYSARL+GISGDPFT+ICISNVKIGL
Sbjct: 361 MTGNYKSHPDEKFDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGL 420

Query: 421 TAKPKKLQWNCTDVEGFSSDVVPPPCAPLAK-AGKSGGCDFPEDQLPIEKVQFKTCFVQN 479
           TAKPKKLQWNCT+VEG+SSDVVP PCAPLAK    S GC FPEDQLPIE V+  TC  +N
Sbjct: 421 TAKPKKLQWNCTNVEGYSSDVVPLPCAPLAKEVVSSDGCPFPEDQLPIEDVKLMTCITKN 478

BLAST of Chy9G171040 vs. ExPASy TrEMBL
Match: A0A6J1H0R5 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111459096 PE=3 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 1.1e-241
Identity = 404/480 (84.17%), Postives = 443/480 (92.29%), Query Frame = 0

Query: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRACP-TNYLQVPAISCRKHTALITDF 60
           MDLP KPTKT   AARIS VILLALVSL +V+GK++CP ++YL+ PAISCRKHTA++TDF
Sbjct: 1   MDLPHKPTKTQITAARISVVILLALVSLGSVDGKKSCPFSSYLEFPAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKI 120
           GG+GDGVTSNT AFR A+EHLS++A DGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDA+I
Sbjct: 61  GGIGDGVTSNTNAFREAMEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQVENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWW 180
           LASQ  +ESEWP V +LPSYG GRDAP GR+SSLIFGTNLTDVVITGNNGTIDGQG+YWW
Sbjct: 121 LASQ--DESEWPLVEVLPSYGRGRDAPDGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWW 180

Query: 181 DKFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDS 240
           DKFHK E N+TRPY+IEIMYS+QIQIS+LTLVNSPSWFVHPIYS+NVIIQGLTILAPIDS
Sbjct: 181 DKFHKNEFNVTRPYMIEIMYSNQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDS 240

Query: 241 PNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDS 300
           PNTDGIDPDSC NTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTEDL+I+RLTCISPDS
Sbjct: 241 PNTDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDS 300

Query: 301 AGIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFW 360
           AGIALGSEMSGGIRNVRIENVT INTQSAVRIKTA GRGG+VKDIFVR M+LSTMKYVFW
Sbjct: 301 AGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFW 360

Query: 361 MTGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGL 420
           MTG+YKSHPD+KFDP ALPEI NINYRDVVAENVTYSARL+GISGDPFT+ICISN+KIGL
Sbjct: 361 MTGDYKSHPDEKFDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNIKIGL 420

Query: 421 TAKPKKLQWNCTDVEGFSSDVVPPPCAPLAK-AGKSGGCDFPEDQLPIEKVQFKTCFVQN 479
           TAK KKLQWNCT+VEG+SSDVVPPPCAPLAK    S GC FPED+LPIE ++  TC  +N
Sbjct: 421 TAKQKKLQWNCTNVEGYSSDVVPPPCAPLAKEVVSSDGCPFPEDRLPIEDLKLMTCVTKN 478

BLAST of Chy9G171040 vs. NCBI nr
Match: XP_011654644.1 (probable polygalacturonase [Cucumis sativus] >KGN49922.1 hypothetical protein Csa_000303 [Cucumis sativus])

HSP 1 Score: 963 bits (2489), Expect = 0.0
Identity = 465/480 (96.88%), Postives = 472/480 (98.33%), Query Frame = 0

Query: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRACPTNYLQVPAISCRKHTALITDFG 60
           M LPQ PTKTHDAAARISAVILLAL+SLATVEGKR+CPTNYLQVPAISCRKHTALITDFG
Sbjct: 1   MYLPQDPTKTHDAAARISAVILLALLSLATVEGKRSCPTNYLQVPAISCRKHTALITDFG 60

Query: 61  GVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKIL 120
           GVGDGVTSNTQAFRRAIEHLSS+AADGGAQL VPPGKWLTGSFNLTSHFTLFVHKDAKIL
Sbjct: 61  GVGDGVTSNTQAFRRAIEHLSSLAADGGAQLTVPPGKWLTGSFNLTSHFTLFVHKDAKIL 120

Query: 121 ASQVENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWD 180
            SQVENESEWPQVA+LPSYG+GRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWD
Sbjct: 121 GSQVENESEWPQVALLPSYGVGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWD 180

Query: 181 KFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP 240
           KFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP
Sbjct: 181 KFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP 240

Query: 241 NTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSA 300
           NTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSA
Sbjct: 241 NTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSA 300

Query: 301 GIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM 360
           G+ALGSEMSGGIRNVRIEN TGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM
Sbjct: 301 GVALGSEMSGGIRNVRIENFTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM 360

Query: 361 TGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT 420
           TGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT
Sbjct: 361 TGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT 420

Query: 421 AKPKKLQWNCTDVEGFSSDVVPPPCAPLAKAGKSGGCDFPEDQLPIEKVQFKTCFVQNLT 480
           A PKKLQWNCTDVEGFSSDVVPPPCAPLAKAGKSGGCDFPEDQLPIE+VQ KTCFVQN T
Sbjct: 421 ATPKKLQWNCTDVEGFSSDVVPPPCAPLAKAGKSGGCDFPEDQLPIERVQLKTCFVQNPT 480

BLAST of Chy9G171040 vs. NCBI nr
Match: XP_008437531.1 (PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042595.1 putative polygalacturonase [Cucumis melo var. makuwa] >TYK05998.1 putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 940 bits (2429), Expect = 0.0
Identity = 454/480 (94.58%), Postives = 469/480 (97.71%), Query Frame = 0

Query: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRACPTNYLQVPAISCRKHTALITDFG 60
           MDLPQKPTKTHDAAARISAVILLALVSLATVEGKR CPTNYLQVPAI+CR+HTA++TDFG
Sbjct: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRQCPTNYLQVPAINCRQHTAVLTDFG 60

Query: 61  GVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKIL 120
           GVGDGVTSNTQAFRRAIEHLS +AA+GGAQLIVPPGKWLTGSFNLTSHFTLFVHKDA IL
Sbjct: 61  GVGDGVTSNTQAFRRAIEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATIL 120

Query: 121 ASQVENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWD 180
           ASQ  +ESEWPQVAILPSYG+GRDAPGGRYSSLI+GTNLTDVVITGNNGTIDGQG+YWWD
Sbjct: 121 ASQ--DESEWPQVAILPSYGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWD 180

Query: 181 KFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP 240
           KFHKGELNLTRPY+IEI+YSDQIQIS+LTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP
Sbjct: 181 KFHKGELNLTRPYMIEILYSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSP 240

Query: 241 NTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSA 300
           NTDGIDPDSCSN RIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVI+RLTCISPDSA
Sbjct: 241 NTDGIDPDSCSNIRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSA 300

Query: 301 GIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM 360
           GIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM
Sbjct: 301 GIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWM 360

Query: 361 TGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT 420
           TGNYKSHPD+KFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT
Sbjct: 361 TGNYKSHPDEKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLT 420

Query: 421 AKPKKLQWNCTDVEGFSSDVVPPPCAPLAKAGKSGGCDFPEDQLPIEKVQFKTCFVQNLT 480
           AKPKKLQWNCTDVEG SSDVVPPPCAPLAKA KSGGCDFP DQLPIEKVQ KTCFVQN T
Sbjct: 421 AKPKKLQWNCTDVEGISSDVVPPPCAPLAKAAKSGGCDFPADQLPIEKVQLKTCFVQNPT 478

BLAST of Chy9G171040 vs. NCBI nr
Match: XP_038906749.1 (probable polygalacturonase [Benincasa hispida])

HSP 1 Score: 905 bits (2338), Expect = 0.0
Identity = 439/479 (91.65%), Postives = 459/479 (95.82%), Query Frame = 0

Query: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRACPT-NYLQVPAISCRKHTALITDF 60
           MDLP+  TK H  AARISAVI+LALVSLATVEGK+ CPT NYL+VPAISCRKHTA++TDF
Sbjct: 1   MDLPKNSTKFHVTAARISAVIILALVSLATVEGKKPCPTTNYLEVPAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKI 120
           GGVGDGVTSNT AFR+AIEHLS +AADGGAQLIVPPGKWLTGSFNLTSHFTLFVHK A I
Sbjct: 61  GGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKYATI 120

Query: 121 LASQVENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWW 180
           LASQ  +ESEWP VA+LPSYG GRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQG+YWW
Sbjct: 121 LASQ--DESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWW 180

Query: 181 DKFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDS 240
           DKFHK ELN+TRPYLIE+MYSDQIQISDLTLVNSPSWFVHPIYS+NVII+GLTILAPIDS
Sbjct: 181 DKFHKDELNVTRPYLIEMMYSDQIQISDLTLVNSPSWFVHPIYSRNVIIRGLTILAPIDS 240

Query: 241 PNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDS 300
           PNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVI+RLTCISPDS
Sbjct: 241 PNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDS 300

Query: 301 AGIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFW 360
           AGIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFW
Sbjct: 301 AGIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFW 360

Query: 361 MTGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGL 420
           MTGNYKSHPDDKFDP A+PEITNINYRDVVAENVTYSARLEGISGDPFT+ICISNVKIGL
Sbjct: 361 MTGNYKSHPDDKFDPTAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGL 420

Query: 421 TAKPKKLQWNCTDVEGFSSDVVPPPCAPLAKAGKSGGCDFPEDQLPIEKVQFKTCFVQN 478
           TAKPKKLQWNCTDVEGFSSDV+PPPCAPLAKA KSGGC FPED+LPIEKVQ KTCFVQN
Sbjct: 421 TAKPKKLQWNCTDVEGFSSDVMPPPCAPLAKAAKSGGCYFPEDELPIEKVQLKTCFVQN 477

BLAST of Chy9G171040 vs. NCBI nr
Match: XP_023532553.1 (probable polygalacturonase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 850 bits (2196), Expect = 3.77e-309
Identity = 410/480 (85.42%), Postives = 445/480 (92.71%), Query Frame = 0

Query: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRACP-TNYLQVPAISCRKHTALITDF 60
           MDLP KPTKT   AAR+SAVILLA VSL +V+GK+ CP ++YL+ PAISCRKHTA++TDF
Sbjct: 1   MDLPHKPTKTQITAARVSAVILLAFVSLGSVDGKKPCPFSSYLEFPAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKI 120
           GGVGDGVTSNT AFR A+EHLS++A DGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDA+I
Sbjct: 61  GGVGDGVTSNTNAFREAMEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQVENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWW 180
           LASQ  +ESEWP V +LPSYG GRDAPGGR+SSLIFGTNLTDVVITGNNGTIDGQG+YWW
Sbjct: 121 LASQ--DESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWW 180

Query: 181 DKFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDS 240
           DKFH+ ELN+TRPY+IEIMYSDQIQIS+LTLVNSPSWFVHPIYS+NVIIQGLTILAPIDS
Sbjct: 181 DKFHRNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDS 240

Query: 241 PNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDS 300
           PNTDGIDPDSC NTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTEDL+I+RLTCISPDS
Sbjct: 241 PNTDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDS 300

Query: 301 AGIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFW 360
           AGIALGSEMSGGIRNVRIENVT INTQSAVRIKTA GRGG+VKDIFVR M+LSTMKYVFW
Sbjct: 301 AGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFW 360

Query: 361 MTGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGL 420
           MTGNYKSHPD+KFDP ALPEI NINYRDVVAENVTYSARL+GISGDPFT+ICISNVKIGL
Sbjct: 361 MTGNYKSHPDEKFDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGL 420

Query: 421 TAKPKKLQWNCTDVEGFSSDVVPPPCAPLAK-AGKSGGCDFPEDQLPIEKVQFKTCFVQN 478
           TAKPKKLQWNCT+VEG+SSDVVPPPCAPLAK    S GC FPED+LPIE V+  TC  +N
Sbjct: 421 TAKPKKLQWNCTNVEGYSSDVVPPPCAPLAKEVVSSDGCPFPEDRLPIEDVKLMTCVTKN 478

BLAST of Chy9G171040 vs. NCBI nr
Match: XP_022996001.1 (probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 845 bits (2182), Expect = 5.12e-307
Identity = 410/480 (85.42%), Postives = 442/480 (92.08%), Query Frame = 0

Query: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRACP-TNYLQVPAISCRKHTALITDF 60
           MDLP KPTKT   A RISAVILL LVSLA+V+GK+ CP ++YL+  AISCRKHTA++TDF
Sbjct: 1   MDLPHKPTKTQITAVRISAVILLVLVSLASVDGKKPCPFSSYLEFSAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTQAFRRAIEHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKI 120
           GGVGDGVTSNT AFR AIEHLS++A DGG+QLIVPPGKWLTGSFNLTSHFTLFVHKDA+I
Sbjct: 61  GGVGDGVTSNTNAFREAIEHLSTLAIDGGSQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQVENESEWPQVAILPSYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWW 180
           LASQ  +ESEWP V +LPSYG GRDAPGGR+SSLIFGTNLTDVVITGNNGTIDGQG+YWW
Sbjct: 121 LASQ--DESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWW 180

Query: 181 DKFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDS 240
           DKFHK E N+TRPY+IEIMYSDQIQIS+LTLVNSPSWFVHPIYS+NVIIQGLTILAPIDS
Sbjct: 181 DKFHKNEFNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDS 240

Query: 241 PNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDS 300
           PNTDGIDPDSC NTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTEDL+I+RLTCISPDS
Sbjct: 241 PNTDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDS 300

Query: 301 AGIALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFW 360
           AGIALGSEMSGGIRNVRIENVT INTQSAVRIKTA GRGG+VKDIFVR M+LSTMKYVFW
Sbjct: 301 AGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFW 360

Query: 361 MTGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGL 420
           MTGNYKSHPD+KFDP ALPEI NINYRDVVAENVTYSARL+GISGDPFT+ICISNVKIGL
Sbjct: 361 MTGNYKSHPDEKFDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGL 420

Query: 421 TAKPKKLQWNCTDVEGFSSDVVPPPCAPLAK-AGKSGGCDFPEDQLPIEKVQFKTCFVQN 478
           TAKPKKLQWNCT+VEG+SSDVVP PCAPLAK    S GC FPEDQLPIE V+  TC  +N
Sbjct: 421 TAKPKKLQWNCTNVEGYSSDVVPLPCAPLAKEVVSSDGCPFPEDQLPIEDVKLMTCITKN 478

BLAST of Chy9G171040 vs. TAIR 10
Match: AT3G48950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 645.6 bits (1664), Expect = 3.2e-185
Identity = 308/463 (66.52%), Postives = 371/463 (80.13%), Query Frame = 0

Query: 20  VILLALVSLATVEGK--RACPTNYLQVPAISCRKHTALITDFGGVGDGVTSNTQAFRRAI 79
           ++ LA+     +E +  R   T+ ++  A++CRKH+A++TDFG VGDG TSNT+AFR AI
Sbjct: 9   LVFLAIFLFPAIESRSHRNSVTSKIEFSALNCRKHSAILTDFGAVGDGKTSNTKAFRNAI 68

Query: 80  EHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQVENESEWPQVAILP 139
             LS MA DGGAQL+VPPGKWLTGSFNLTSHFTLF+ + A ILASQ  +ESEWP +A LP
Sbjct: 69  SKLSQMATDGGAQLVVPPGKWLTGSFNLTSHFTLFIQRGATILASQ--DESEWPVIAPLP 128

Query: 140 SYGIGRDAPG-GRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWDKFHKGELNLTRPYLIE 199
           SYG GRD  G GR++SLI GTNLTDVVITGNNGTI+GQG YWWDKF K +  +TRPYLIE
Sbjct: 129 SYGKGRDGTGTGRFNSLISGTNLTDVVITGNNGTINGQGQYWWDKFKKKQFKITRPYLIE 188

Query: 200 IMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIE 259
           I++S  IQIS++TL++SPSW +HP+Y  +VI++ +T+LAP+  PNTDGI+PDSC+NT IE
Sbjct: 189 ILFSKNIQISNITLIDSPSWNIHPVYCNSVIVKSVTVLAPVTVPNTDGINPDSCTNTLIE 248

Query: 260 DCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSAGIALGSEMSGGIRNVR 319
           DC+IVSGDDCIAVKSGWDQYGIKFGMPT+ L I+RLTCISP SAG+ALGSEMSGGI++VR
Sbjct: 249 DCYIVSGDDCIAVKSGWDQYGIKFGMPTQQLSIRRLTCISPKSAGVALGSEMSGGIKDVR 308

Query: 320 IENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPAA 379
           IE+VT  NT+SA+RIKTA GRG +VKDI+ R + + TMKYVFWM+GNY SHPD+ FDP A
Sbjct: 309 IEDVTLTNTESAIRIKTAVGRGAYVKDIYARRITMKTMKYVFWMSGNYGSHPDEGFDPKA 368

Query: 380 LPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGF 439
           LPEITNINYRD+ AENVT SA L+GI  DPFT ICISNV I L AK KK+QWNCTDV G 
Sbjct: 369 LPEITNINYRDMTAENVTMSASLDGIDKDPFTGICISNVTIALAAKAKKMQWNCTDVAGV 428

Query: 440 SSDVVPPPCA--PLAKA-GKSGGCDFPEDQLPIEKVQFKTCFV 477
           +S V P PC+  P  KA  K+  C FP D +PIE V  K CF+
Sbjct: 429 TSRVTPEPCSLLPEKKAQAKNVDCAFPSDLIPIESVVLKKCFL 469

BLAST of Chy9G171040 vs. TAIR 10
Match: AT3G61490.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 632.5 bits (1630), Expect = 2.8e-181
Identity = 301/468 (64.32%), Postives = 369/468 (78.85%), Query Frame = 0

Query: 20  VILLALVSLATV-EGKRACPT-NYLQVPAISCRKHTALITDFGGVGDGVTSNTQAFRRAI 79
           V ++ L +L T+ +G+R   +    +  AI CR H+A IT++GGVGDG T NT+AF+ A+
Sbjct: 9   VTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAFQSAV 68

Query: 80  EHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQVENESEWPQVAILP 139
           +HLS  +++GGAQL VP GKWLTGSFNLTSHFTLF+HKDA +LA+Q  N  E+P +  LP
Sbjct: 69  DHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLN--EYPILKALP 128

Query: 140 SYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWDKFHKGELNLTRPYLIEI 199
           SYG GRDA GGR++SLIFGTNL+DV+ITGNNGTIDGQG++WW KFH G+L  TRPYLIE+
Sbjct: 129 SYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLIEL 188

Query: 200 MYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIED 259
           M+SD IQIS+LT ++SPSW +HP+YS N+I++G+TI+AP+ SPNTDGI+PDSC+NTRIED
Sbjct: 189 MFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRIED 248

Query: 260 CFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSAGIALGSEMSGGIRNVRI 319
           C+I+SGDDCIAVKSGWD+YGI FGMPT+ LVI+RLTCISP SA IALGSEMSGGI +VR 
Sbjct: 249 CYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDVRA 308

Query: 320 ENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPAAL 379
           E++T   T+S VRIKTA GRG FVK+I+V+GM L TMK+VFWMTGNYK+H D  +DP AL
Sbjct: 309 EDITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPHAL 368

Query: 380 PEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGFS 439
           PEIT INYRD+VAENV+ + RLEGISGDPFT ICISN  I + AK KK  W C+DVEG +
Sbjct: 369 PEITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEGVT 428

Query: 440 SDVVPPPCAPL--------AKAGKSGGCDFPEDQLPIEKVQFKTCFVQ 478
           S V P PC  L         K    GGCDFP D L I+ V+ KTC  Q
Sbjct: 429 SGVDPKPCDLLDGQESETTKKKMIDGGCDFPTDVLEIDNVELKTCSYQ 474

BLAST of Chy9G171040 vs. TAIR 10
Match: AT3G61490.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 632.5 bits (1630), Expect = 2.8e-181
Identity = 301/468 (64.32%), Postives = 369/468 (78.85%), Query Frame = 0

Query: 20  VILLALVSLATV-EGKRACPT-NYLQVPAISCRKHTALITDFGGVGDGVTSNTQAFRRAI 79
           V ++ L +L T+ +G+R   +    +  AI CR H+A IT++GGVGDG T NT+AF+ A+
Sbjct: 9   VTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAFQSAV 68

Query: 80  EHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQVENESEWPQVAILP 139
           +HLS  +++GGAQL VP GKWLTGSFNLTSHFTLF+HKDA +LA+Q  N  E+P +  LP
Sbjct: 69  DHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLN--EYPILKALP 128

Query: 140 SYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWDKFHKGELNLTRPYLIEI 199
           SYG GRDA GGR++SLIFGTNL+DV+ITGNNGTIDGQG++WW KFH G+L  TRPYLIE+
Sbjct: 129 SYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLIEL 188

Query: 200 MYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIED 259
           M+SD IQIS+LT ++SPSW +HP+YS N+I++G+TI+AP+ SPNTDGI+PDSC+NTRIED
Sbjct: 189 MFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRIED 248

Query: 260 CFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSAGIALGSEMSGGIRNVRI 319
           C+I+SGDDCIAVKSGWD+YGI FGMPT+ LVI+RLTCISP SA IALGSEMSGGI +VR 
Sbjct: 249 CYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDVRA 308

Query: 320 ENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPAAL 379
           E++T   T+S VRIKTA GRG FVK+I+V+GM L TMK+VFWMTGNYK+H D  +DP AL
Sbjct: 309 EDITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPHAL 368

Query: 380 PEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGFS 439
           PEIT INYRD+VAENV+ + RLEGISGDPFT ICISN  I + AK KK  W C+DVEG +
Sbjct: 369 PEITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEGVT 428

Query: 440 SDVVPPPCAPL--------AKAGKSGGCDFPEDQLPIEKVQFKTCFVQ 478
           S V P PC  L         K    GGCDFP D L I+ V+ KTC  Q
Sbjct: 429 SGVDPKPCDLLDGQESETTKKKMIDGGCDFPTDVLEIDNVELKTCSYQ 474

BLAST of Chy9G171040 vs. TAIR 10
Match: AT3G61490.3 (Pectin lyase-like superfamily protein )

HSP 1 Score: 632.5 bits (1630), Expect = 2.8e-181
Identity = 301/468 (64.32%), Postives = 369/468 (78.85%), Query Frame = 0

Query: 20  VILLALVSLATV-EGKRACPT-NYLQVPAISCRKHTALITDFGGVGDGVTSNTQAFRRAI 79
           V ++ L +L T+ +G+R   +    +  AI CR H+A IT++GGVGDG T NT+AF+ A+
Sbjct: 9   VTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAFQSAV 68

Query: 80  EHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQVENESEWPQVAILP 139
           +HLS  +++GGAQL VP GKWLTGSFNLTSHFTLF+HKDA +LA+Q  N  E+P +  LP
Sbjct: 69  DHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLN--EYPILKALP 128

Query: 140 SYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWDKFHKGELNLTRPYLIEI 199
           SYG GRDA GGR++SLIFGTNL+DV+ITGNNGTIDGQG++WW KFH G+L  TRPYLIE+
Sbjct: 129 SYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLIEL 188

Query: 200 MYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIED 259
           M+SD IQIS+LT ++SPSW +HP+YS N+I++G+TI+AP+ SPNTDGI+PDSC+NTRIED
Sbjct: 189 MFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRIED 248

Query: 260 CFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSAGIALGSEMSGGIRNVRI 319
           C+I+SGDDCIAVKSGWD+YGI FGMPT+ LVI+RLTCISP SA IALGSEMSGGI +VR 
Sbjct: 249 CYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDVRA 308

Query: 320 ENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPAAL 379
           E++T   T+S VRIKTA GRG FVK+I+V+GM L TMK+VFWMTGNYK+H D  +DP AL
Sbjct: 309 EDITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPHAL 368

Query: 380 PEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGFS 439
           PEIT INYRD+VAENV+ + RLEGISGDPFT ICISN  I + AK KK  W C+DVEG +
Sbjct: 369 PEITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEGVT 428

Query: 440 SDVVPPPCAPL--------AKAGKSGGCDFPEDQLPIEKVQFKTCFVQ 478
           S V P PC  L         K    GGCDFP D L I+ V+ KTC  Q
Sbjct: 429 SGVDPKPCDLLDGQESETTKKKMIDGGCDFPTDVLEIDNVELKTCSYQ 474

BLAST of Chy9G171040 vs. TAIR 10
Match: AT2G23900.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 620.5 bits (1599), Expect = 1.1e-177
Identity = 294/459 (64.05%), Postives = 361/459 (78.65%), Query Frame = 0

Query: 20  VILLALVSLATVEGKRAC---PTNYLQVPAISCRKHTALITDFGGVGDGVTSNTQAFRRA 79
           +++LA+ S + +E +       TN ++  A++CRKHTA++T+FG VGDG TSNT+AF+ A
Sbjct: 20  LVVLAISSFSMMEARDLASKGKTN-IEYMALNCRKHTAVLTEFGAVGDGKTSNTKAFKEA 79

Query: 80  IEHLSSMAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAKILASQVENESEWPQVAIL 139
           I  L+  AADGG QLIVPPGKWLTGSFNLTSHFTLF+ K A ILASQ  +ESE+P VA L
Sbjct: 80  ITKLAPKAADGGVQLIVPPGKWLTGSFNLTSHFTLFIQKGATILASQ--DESEYPVVAPL 139

Query: 140 PSYGIGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWDKFHKGEL-NLTRPYLI 199
           PSYG GRDA G  ++SLI GTNLTDVVITGNNGTI+GQG YWW K+  G    +TRPY I
Sbjct: 140 PSYGQGRDAAGPTFASLISGTNLTDVVITGNNGTINGQGKYWWVKYRSGGFKGITRPYTI 199

Query: 200 EIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNTRI 259
           EI++S  +QIS++T+++SP+W +HP+Y  NVI++G+TILAPIDSPNTDGI+PDSC+NT I
Sbjct: 200 EIIFSQNVQISNITIIDSPAWNIHPVYCNNVIVKGVTILAPIDSPNTDGINPDSCTNTLI 259

Query: 260 EDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSAGIALGSEMSGGIRNV 319
           EDC++VSGDDCIAVKSGWDQ+GIK GMPT+ L I+RLTCISPDSAGIALGSEMSGGI++V
Sbjct: 260 EDCYVVSGDDCIAVKSGWDQFGIKVGMPTQQLSIRRLTCISPDSAGIALGSEMSGGIKDV 319

Query: 320 RIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPA 379
           RIE++T + TQSA+RIKTA GRGG+VKDIF R   + TMKYVFWM+G Y  HP   FDP 
Sbjct: 320 RIEDITLLQTQSAIRIKTAVGRGGYVKDIFARRFTMKTMKYVFWMSGAYNQHPASGFDPK 379

Query: 380 ALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEG 439
           A+P ITNINYRD+ A+NVT  ARL+G   DPFT IC+SN+KI L A+PKKL WNCT + G
Sbjct: 380 AMPVITNINYRDMTADNVTQPARLDGFKNDPFTKICMSNIKIDLAAEPKKLLWNCTSISG 439

Query: 440 FSSDVVPPPCAPLAKAGKSGGCDFPEDQLPIEKVQFKTC 475
            SS V P PC+ L + G    C FP D++PIE V    C
Sbjct: 440 VSSKVTPKPCSLLPEKGAPVDCAFPVDKIPIESVVLNKC 475

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A7PZL37.5e-17964.55Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ... [more]
Q9LW075.0e-3430.02Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q7M1E73.2e-2828.61Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Q9FY191.0e-2626.96Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
Q8RY294.3e-2524.51Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KNF95.0e-27996.88Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G140480 PE=3 SV=1[more]
A0A5D3C4614.6e-27294.58Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3AUV54.6e-27294.58probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482916 PE=3 SV=1[more]
A0A6J1K3G82.6e-24385.42probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111491343 PE=3 SV=1[more]
A0A6J1H0R51.1e-24184.17probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111459096 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_011654644.10.096.88probable polygalacturonase [Cucumis sativus] >KGN49922.1 hypothetical protein Cs... [more]
XP_008437531.10.094.58PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042595.1 putative poly... [more]
XP_038906749.10.091.65probable polygalacturonase [Benincasa hispida][more]
XP_023532553.13.77e-30985.42probable polygalacturonase [Cucurbita pepo subsp. pepo][more]
XP_022996001.15.12e-30785.42probable polygalacturonase [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G48950.13.2e-18566.52Pectin lyase-like superfamily protein [more]
AT3G61490.12.8e-18164.32Pectin lyase-like superfamily protein [more]
AT3G61490.22.8e-18164.32Pectin lyase-like superfamily protein [more]
AT3G61490.32.8e-18164.32Pectin lyase-like superfamily protein [more]
AT2G23900.11.1e-17764.05Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 284..306
e-value: 2600.0
score: 4.2
coord: 312..333
e-value: 750.0
score: 8.1
coord: 223..249
e-value: 1100.0
score: 7.0
coord: 154..180
e-value: 5600.0
score: 1.9
coord: 250..271
e-value: 350.0
score: 10.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 36..439
e-value: 7.9E-113
score: 379.5
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 152..434
e-value: 9.6E-40
score: 136.6
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 17..475
NoneNo IPR availablePANTHERPTHR31339:SF12ENDO-POLYGALACTURONASE-LIKE PROTEINcoord: 17..475
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 45..475

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy9G171040.1Chy9G171040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity