Chy9G168470 (gene) Cucumber (hystrix) v1

Overview
NameChy9G168470
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Description7-ethoxycoumarin O-deethylase-like
LocationchrH09: 13555284 .. 13557617 (+)
RNA-Seq ExpressionChy9G168470
SyntenyChy9G168470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAATCTCAGCTCTCTTCTTCTTCATTCTCCCCTCATTTTGGTCTACACATTCCAATGAAAACAACCAAATTTCAATCTCCATTCTCTTAGTCTTCCTCCTCCTCTTTCTATTCTGGTTCCGACCCAAATTCTGGCGTCCCTGTTTGCCTCCAGGTCCCTGTGGCCTGCCACTGGTCGGTTACCTTCCATTTTTATCAGGCAATCCCCACCACAAATTAACCCATTTGGCTAAAATCTACGGCCCTGTTTTCAAGCTCCGTCTTGGGCCAAAGCTCTGCGTCGTTCTCACTTCGCCTGCTTTCATCAAAGAAGTCTTCCACCACCAAGAAACTCTCTTTCCCAATCGAAACACCACCGTTTGTGCTCTTCTCTCCAGCTCCGGCCGCTCCGGCATTGTCTTTGGCCAGGATGGGGAGGATTGGAAGAAGCTAAGAAAAATCTTCGTTCGGAAAATGATTAGCAAGTCCAACCTCGACGCGTCCTACTGTGTTCGAAGACAAGAGGTGAGAATCGAATCGGTGGGAACACAAATAGACATTGGAAAGGTGAGTTTCCTTGCGACAATGAAATCAGTTATAGCAATGACATGGGGCGATTCGGGGCGGGTGATGGGAGAGGATGGGGGTTGGTTTGGAAGTGAAGTTTAGGGAAATGATGGTTGAACTGATGGTGTTGCTTGGAAGTCGGAATGTATCGGATGTTTTTCCGGTGTTGGGTCGGTTTGATTTGCAGGGAATTGGAAGGAGGACGAAGAAGGTGATGGGTGTGTTGGATGGGATTCTGAATTCAGTCATTGAAGAGCAGAGGAAGATGGGAGGAAATGGTGGGGGAAAGGAGGGTACTTGCAGTTGTTGTTGGAGCTTCAGGATAATGAAGATAATTCAGAGTGCATTACGAACGACCAACTCAAAGCTTTGCTATTGGTATGATTTTCTTGGAAAAAATGCATCTCATCTTTATAAATAATAATTATAATAATTACAGTAAAAAACCATTCATGCATCAAAACTCCATTAAATAAGCGCATAAGCATTTAATTTCTAGCAATTTGATGCGAAACATTTCCGAGATGGAGCCAAAAAAAAAAAGCCTCCATACATATAATAGAAGCAGATCTAATAGAACAGCCAACAACGTTTAAACAAACTTGAAATTTGGGATTTGAATTCTACTCTGATGCTTTTTTTTCTCTTTCCTCTTTACTTTTAATCCATTTTTTGTTGTTTCTTTTTTCTTAAAATTCAAAATAACGAAACCAAATTTTATTGTTTCTTTTTATTTTACAATTTTTTGTTGCTTAAAACTTTTTAATATAACCAGATAAATTTCATTTTTATTTTTTCTCATATATATGTGTTCATGTTTGTATAAATTTTTTTATTATAAAATTTGTTTATAATAAAATGTACACCTATCTATCTCGTGTTTCACATTTTTCCACCCTAGAAAAATAATTCTTTTAAAATATTTGACTAACTTTTATTTTTGTATGCTAGAGAGATGAGAAGCACATAAGATAGTTGTATCTTAGATGTACTCAAGTTTTTTCTTTCGAAATTATATAAAAATATTATTTTTAAATTTCAAGAACTAAAAGTATTCCTAATTTTGTTTGGTTATTATAGTTTAAAACCATTATCTTTTGATTTGTTATGTTATTTTTCTGTAATACACAGGACATTGTGATTGGTGGAACAGAAACAACATCGACTACAATTGAGTGGGCAATGGCAGAATTAATGCAACATCCAGATGCAATGAAGAAAGTGAAAGAAGAACTAAAGGAAGTGATAGGTTTAAACGCAATAGTGGAAGAATTTCACTTCTCCAAATTATGCTATTTAAATGCTGTGATTAAAGAAACACTTCGCTTACATCCAGCTATCTTTCTATTAGTGCCTCGAACTCTTACTTCCTCGACCACTCTTGGAGGGTACTACATCCCAAAAGATTCAACCATCTACTTCAAACTTTGGGGCATTCAAAGAGATCCTACAATTTGGGATAATCCCTCAAAATTCATGCCAGAAAGGTTCGTAAAAAGTAACGAAGAAGAATGTGCTGGAGAATTTGAATTGGGTGATAGTAATAATAATGCCATGGAGTTTTACCCATTTGGATATGGCAAAAGATCATGTGCAGGGATCGCATTGGCCGAGAGGATGCTGATGTTCATATTGGCATCTTTGCTGCATTCTTTCGAGTGGGAATTGCCTAAGGATTCAGTGATTGATTTTAAAGAGAAATTTGGAATTGTCAATAAGAAGTTGAACCCTTTGGTTGCTATTCCTACGCCAACACTCTCCAATTCGGACCTTTACTTGGCCTAA

mRNA sequence

ATGTCAATCTCAGCTCTCTTCTTCTTCATTCTCCCCTCATTTTGGTCTACACATTCCAATGAAAACAACCAAATTTCAATCTCCATTCTCTTAGTCTTCCTCCTCCTCTTTCTATTCTGGTTCCGACCCAAATTCTGGCGTCCCTGTTTGCCTCCAGGTCCCTGTGGCCTGCCACTGGTCGGTTACCTTCCATTTTTATCAGGCAATCCCCACCACAAATTAACCCATTTGGCTAAAATCTACGGCCCTGTTTTCAAGCTCCGTCTTGGGCCAAAGCTCTGCGTCGTTCTCACTTCGCCTGCTTTCATCAAAGAAGTCTTCCACCACCAAGAAACTCTCTTTCCCAATCGAAACACCACCGTTTGTGCTCTTCTCTCCAGCTCCGGCCGCTCCGGCATTGTCTTTGGCCAGGATGGGGAGGATTGGAAGAAGCTAAGAAAAATCTTCGTTCGGAAAATGATTAGCAAGTCCAACCTCGACGCGTCCTACTGTGTTCGAAGACAAGAGGTGAGAATCGAATCGGTGGGAACACAAATAGACATTGGAAAGGTGAGTTTCCTTGCGACAATGAAATCAGTTATAGCAATGACATGGGGCGATTCGGGGCGGGTGATGGGAGAGGATGGGGAGGAAGATGGGAGGAAATGGTGGGGGAAAGGAGGGTACTTGCAGTTGTTGTTGGAGCTTCAGGATAATGAAGATAATTCAGAGTGCATTACGAACGACCAACTCAAAGCTTTGCTATTGGACATTGTGATTGGTGGAACAGAAACAACATCGACTACAATTGAGTGGGCAATGGCAGAATTAATGCAACATCCAGATGCAATGAAGAAAGTGAAAGAAGAACTAAAGGAAGTGATAGGTTTAAACGCAATAGTGGAAGAATTTCACTTCTCCAAATTATGCTATTTAAATGCTGTGATTAAAGAAACACTTCGCTTACATCCAGCTATCTTTCTATTAGTGCCTCGAACTCTTACTTCCTCGACCACTCTTGGAGGGTACTACATCCCAAAAGATTCAACCATCTACTTCAAACTTTGGGGCATTCAAAGAGATCCTACAATTTGGGATAATCCCTCAAAATTCATGCCAGAAAGGTTCGTAAAAAGTAACGAAGAAGAATGTGCTGGAGAATTTGAATTGGGTGATAGTAATAATAATGCCATGGAGTTTTACCCATTTGGATATGGCAAAAGATCATGTGCAGGGATCGCATTGGCCGAGAGGATGCTGATGTTCATATTGGCATCTTTGCTGCATTCTTTCGAGTGGGAATTGCCTAAGGATTCAGTGATTGATTTTAAAGAGAAATTTGGAATTGTCAATAAGAAGTTGAACCCTTTGGTTGCTATTCCTACGCCAACACTCTCCAATTCGGACCTTTACTTGGCCTAA

Coding sequence (CDS)

ATGTCAATCTCAGCTCTCTTCTTCTTCATTCTCCCCTCATTTTGGTCTACACATTCCAATGAAAACAACCAAATTTCAATCTCCATTCTCTTAGTCTTCCTCCTCCTCTTTCTATTCTGGTTCCGACCCAAATTCTGGCGTCCCTGTTTGCCTCCAGGTCCCTGTGGCCTGCCACTGGTCGGTTACCTTCCATTTTTATCAGGCAATCCCCACCACAAATTAACCCATTTGGCTAAAATCTACGGCCCTGTTTTCAAGCTCCGTCTTGGGCCAAAGCTCTGCGTCGTTCTCACTTCGCCTGCTTTCATCAAAGAAGTCTTCCACCACCAAGAAACTCTCTTTCCCAATCGAAACACCACCGTTTGTGCTCTTCTCTCCAGCTCCGGCCGCTCCGGCATTGTCTTTGGCCAGGATGGGGAGGATTGGAAGAAGCTAAGAAAAATCTTCGTTCGGAAAATGATTAGCAAGTCCAACCTCGACGCGTCCTACTGTGTTCGAAGACAAGAGGTGAGAATCGAATCGGTGGGAACACAAATAGACATTGGAAAGGTGAGTTTCCTTGCGACAATGAAATCAGTTATAGCAATGACATGGGGCGATTCGGGGCGGGTGATGGGAGAGGATGGGGAGGAAGATGGGAGGAAATGGTGGGGGAAAGGAGGGTACTTGCAGTTGTTGTTGGAGCTTCAGGATAATGAAGATAATTCAGAGTGCATTACGAACGACCAACTCAAAGCTTTGCTATTGGACATTGTGATTGGTGGAACAGAAACAACATCGACTACAATTGAGTGGGCAATGGCAGAATTAATGCAACATCCAGATGCAATGAAGAAAGTGAAAGAAGAACTAAAGGAAGTGATAGGTTTAAACGCAATAGTGGAAGAATTTCACTTCTCCAAATTATGCTATTTAAATGCTGTGATTAAAGAAACACTTCGCTTACATCCAGCTATCTTTCTATTAGTGCCTCGAACTCTTACTTCCTCGACCACTCTTGGAGGGTACTACATCCCAAAAGATTCAACCATCTACTTCAAACTTTGGGGCATTCAAAGAGATCCTACAATTTGGGATAATCCCTCAAAATTCATGCCAGAAAGGTTCGTAAAAAGTAACGAAGAAGAATGTGCTGGAGAATTTGAATTGGGTGATAGTAATAATAATGCCATGGAGTTTTACCCATTTGGATATGGCAAAAGATCATGTGCAGGGATCGCATTGGCCGAGAGGATGCTGATGTTCATATTGGCATCTTTGCTGCATTCTTTCGAGTGGGAATTGCCTAAGGATTCAGTGATTGATTTTAAAGAGAAATTTGGAATTGTCAATAAGAAGTTGAACCCTTTGGTTGCTATTCCTACGCCAACACTCTCCAATTCGGACCTTTACTTGGCCTAA

Protein sequence

MSISALFFFILPSFWSTHSNENNQISISILLVFLLLFLFWFRPKFWRPCLPPGPCGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHHQETLFPNRNTTVCALLSSSGRSGIVFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVRIESVGTQIDIGKVSFLATMKSVIAMTWGDSGRVMGEDGEEDGRKWWGKGGYLQLLLELQDNEDNSECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLNAIVEEFHFSKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGIQRDPTIWDNPSKFMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWELPKDSVIDFKEKFGIVNKKLNPLVAIPTPTLSNSDLYLA*
Homology
BLAST of Chy9G168470 vs. ExPASy Swiss-Prot
Match: A0A0S1TPC7 (Carnosic acid synthase OS=Salvia pomifera OX=396869 GN=CYP76AK6 PE=1 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 8.2e-66
Identity = 159/478 (33.26%), Postives = 233/478 (48.74%), Query Frame = 0

Query: 50  LPPGPCGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHH 109
           LPPGP  LP++G +  L  NPH  L HLAK YGP+  L+LG +  VV++SP   +EV   
Sbjct: 32  LPPGPPRLPIIGNMLQLGQNPHKSLAHLAKTYGPLMSLKLGNQFVVVVSSPEMAREVLQR 91

Query: 110 QETLFPNRNTTVCALLSSSGRSGI-VFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQ 169
              +F    T +   +   G   + +       WKK+RKI   K+ S   L A+  VRR+
Sbjct: 92  HGLVFSRPFTPIAVQILGHGEVSMNMLPATSPIWKKIRKIAREKLFSNQALHATRAVRRE 151

Query: 170 EVR-----IESVGTQIDIGKVSF-------LATMKSVIAMTWGDSG-------------- 229
            +R     +      +++G+ +F        AT+ SV    + DS               
Sbjct: 152 RLRKLADYVGRCSGAMNVGEATFTTMSNLMFATLFSVEITQYADSDSDSGVNKKFREHVN 211

Query: 230 ---RVMGEDGEED----------------------------------------GRKWWGK 289
              R MG     D                                           +  K
Sbjct: 212 AITRYMGVPNIADFFPIFAPFDPQGLRRKLTYHLGSLLELVQSLIEQRLRARNAATYRKK 271

Query: 290 GGYLQLLLELQDNEDNSECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKK 349
             +L++LL+L + ++    ++ +++K L +D++I G++T++ T EWAM EL+ HPD + K
Sbjct: 272 DDFLEMLLDLSEGDEYD--LSVNEIKHLCVDLIIAGSDTSAATTEWAMVELLLHPDKLAK 331

Query: 350 VKEELKEVIGLNAIVEEFHFSKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIP 409
           +K ELK V+G  +I+EE   SKL YL A +KE LR HPA  LL P      T L GY IP
Sbjct: 332 LKAELKSVVGDKSIIEESDISKLPYLQATVKEVLRYHPAAPLLAPHLAEEETQLNGYIIP 391

Query: 410 KDSTIYFKLWGIQRDPTIWDNPSKFMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYG 455
           K++ I+   W I RDP+IW NP  F PERF+ ++ + C   FEL           PFG G
Sbjct: 392 KNTKIFINDWTISRDPSIWKNPEMFEPERFLNNDIDFCGQHFEL----------IPFGSG 451

BLAST of Chy9G168470 vs. ExPASy Swiss-Prot
Match: D1MI46 (Geraniol 8-hydroxylase OS=Swertia mussotii OX=137888 GN=CYP76B10 PE=1 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.0e-63
Identity = 159/475 (33.47%), Postives = 238/475 (50.11%), Query Frame = 0

Query: 50  LPPGPCGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHH 109
           LPPGP  LPL+G L  L   PH  L  LAK +GP+  L+LG    +V+TS    KEV   
Sbjct: 32  LPPGPSPLPLIGNLHLLGDQPHKSLAKLAKKHGPIMGLQLGQVTTIVVTSSGMAKEVLQK 91

Query: 110 QETLFPNRNTTVCALLSSSGRSGIVFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQE 169
           Q+  F +R+           +  +++      W+ LRK     M S + LDA+  +R ++
Sbjct: 92  QDLAFSSRSIPNAIHAHDQYKYSVIWLPVASRWRGLRKALNSNMFSGNRLDANQHLRSRK 151

Query: 170 V--------RIESVGTQIDIGKVSFLATMKSV--------IAMTWGDSGR--------VM 229
           V        +    G  ID+G+ +F  ++  +        +   + DS +        VM
Sbjct: 152 VQELIAYCRKSSQTGDAIDVGRAAFRTSLNLLSNTMFSKDLTDPYSDSAKEFKDLVWNVM 211

Query: 230 GEDGE---------------EDGRK----WWGK-----GGYLQLLLE------------- 289
            E G+               +  RK     +GK     GG +   L+             
Sbjct: 212 VEAGKPNLVDYFPLLDKVDPQGIRKRMTIHFGKILELFGGLIDERLQQKKAKGVNDDVLD 271

Query: 290 --LQDNEDNSECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEELKE 349
             L  +E++ E I    ++ + LD+ + GT+TTS+T+EWAM+E++++P+ MK  + EL +
Sbjct: 272 VLLTTSEESPEEIDRTHIQRMCLDLFVAGTDTTSSTLEWAMSEMLKNPEKMKAAQAELAQ 331

Query: 350 VIGLNAIVEEFHFSKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYF 409
           VIG    VEE   ++L YL   IKETLR+HP + LL+PR       + GY +PK+S +  
Sbjct: 332 VIGKGKAVEEADLARLPYLRCAIKETLRIHPPVPLLIPRRTEQEVEVCGYTVPKNSQVLV 391

Query: 410 KLWGIQRDPTIWDNPSKFMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYGKRSCAGI 457
            +W I RD  IW +P  F PERF++S  E    +FEL           PFG G+R C G+
Sbjct: 392 NVWAISRDDAIWKDPLSFKPERFLESELEMRGKDFEL----------IPFGAGRRICPGL 451

BLAST of Chy9G168470 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.2e-63
Identity = 160/501 (31.94%), Postives = 242/501 (48.30%), Query Frame = 0

Query: 28  SILLVFLLLFLFWFRPKFWRPCLPPGPCGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKL 87
           ++LL   +++L + R     P LPPGP G P++G LP L   PH  L  L+K YGP+F+L
Sbjct: 11  TLLLSSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKPHQTLDALSKQYGPLFRL 70

Query: 88  RLGPKLCVVLTSPAFIKEVFHHQETLFPNRNTTVCALLSSSGRSGIVFGQDGEDWKKLRK 147
           RLG    VV +S A   +     +  F NR     A   +     +VF   G  W+ LRK
Sbjct: 71  RLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPRWRMLRK 130

Query: 148 IFVRKMISKSNLDASYCVRRQEV--------RIESVGTQIDIGK-VSFLATMKSVIAMT- 207
           +    + S   LD    VR+ EV        R    G  +++GK ++  AT     AM  
Sbjct: 131 LCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCATNALARAMLG 190

Query: 208 -----------------------------------------WGDSGRVMGEDGE------ 267
                                                    W D   V+G+         
Sbjct: 191 RRVFADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKRLHRRYD 250

Query: 268 -------EDGRKWWGKGGYLQLLLELQDNEDNSEC-ITNDQLKALLLDIVIGGTETTSTT 327
                  E+ +     G  L +L+ L++ +   E  + N  +KALLL++   GT+T+S+T
Sbjct: 251 AFLDRVIEENQANAKSGDLLSVLIRLKEADAEGEIKLNNTDIKALLLNLFTAGTDTSSST 310

Query: 328 IEWAMAELMQHPDAMKKVKEELKEVIGLNAIVEEFHFSKLCYLNAVIKETLRLHPAIFLL 387
           +EW +AEL++HPD ++K + EL  VIG + +V E     L YL AV+KET RLHP+  L 
Sbjct: 311 VEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPYLQAVVKETFRLHPSTPLS 370

Query: 388 VPRTLTSSTTLGGYYIPKDSTIYFKLWGIQRDPTIWDNPSKFMPERFVKSNEEECAGEFE 447
           +PR  +    + GY IPK +T+   +W I RD  +W++P +F P RF+        GE E
Sbjct: 371 LPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEFRPSRFLP------GGERE 430

Query: 448 LGDSNNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWELPKD---SVIDFKEK 461
             D   N  E  PFG G+R CAG++L  RM+ F+ A+++H+++W LPK      +D +E 
Sbjct: 431 HVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPKGQECQKLDMEEA 490

BLAST of Chy9G168470 vs. ExPASy Swiss-Prot
Match: A0A1D8QMD1 (Carnosic acid synthase OS=Salvia fruticosa OX=268906 GN=CYP76AK6 PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 5.0e-63
Identity = 153/478 (32.01%), Postives = 230/478 (48.12%), Query Frame = 0

Query: 50  LPPGPCGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHH 109
           LPPGP  LP++G +  L  NPH  L HLAK YGP+  L+LG +  VV++SP   +EV   
Sbjct: 32  LPPGPPRLPIIGNMLQLGQNPHKSLAHLAKTYGPLMSLKLGNQFVVVVSSPEMAREVLQR 91

Query: 110 QETLFPNRNTTVCALLSSSGRSGI-VFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQ 169
              +F      +   +   G   + +       WKKLRKI   K+ S   L  +  VR +
Sbjct: 92  HGLVFSTPCAPIAVQILGHGEVSMNMLPATSPIWKKLRKIAREKLFSNQALHDTRAVRWE 151

Query: 170 EVR-----IESVGTQIDIGKVSF-------LATMKSVIAMTWGDSGRVMGEDGE------ 229
            +R     +      +++G+ +F        +T+ SV    + DS    G + +      
Sbjct: 152 RLRKLADYVGRCSGAMNVGEATFTTMSNLMFSTLFSVEITQYADSDSDSGVNKKFREHVN 211

Query: 230 ---------------------------------------------------EDGRKWWGK 289
                                                               +   +  K
Sbjct: 212 AITRYIGVPNIADFFPIFAPFDPQGLRRKLTYHFGSLLELVQSLIEQRLRARNAATYRKK 271

Query: 290 GGYLQLLLELQDNEDNSECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKK 349
             +L++LL+L + ++    ++ +++K L +D++I G++T++ T EWAM EL+ HPD + K
Sbjct: 272 DDFLEMLLDLSEGDEYD--LSVNEIKHLCVDLIIAGSDTSAATTEWAMVELLLHPDKLAK 331

Query: 350 VKEELKEVIGLNAIVEEFHFSKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIP 409
           +K ELK V+G  +I+EE   SKL YL A +KE LR HPA  LL P      T L GY IP
Sbjct: 332 LKAELKSVVGDKSIIEESDISKLPYLQATVKEVLRYHPAAPLLAPHLAEEETQLNGYIIP 391

Query: 410 KDSTIYFKLWGIQRDPTIWDNPSKFMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYG 455
           K++ I+   W I RDP+IW NP  F PERF+ ++ + C   FEL           PFG G
Sbjct: 392 KNTKIFINDWTISRDPSIWKNPEMFEPERFLDNDIDFCGQHFEL----------IPFGSG 451

BLAST of Chy9G168470 vs. ExPASy Swiss-Prot
Match: Q8VWZ7 (Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 5.5e-62
Identity = 149/476 (31.30%), Postives = 235/476 (49.37%), Query Frame = 0

Query: 50  LPPGPCGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHH 109
           LPPGP  LP +G L  L   PH  L  L+K +GP+  L+LG    +V++S    KEV   
Sbjct: 30  LPPGPSPLPFIGSLHLLGDQPHKSLAKLSKKHGPIMSLKLGQITTIVISSSTMAKEVLQK 89

Query: 110 QETLFPNRNTTVCALLSSSGRSGIVFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQE 169
           Q+  F +R+        +  +  +V+      W+ LRK+    + S + LDA+  +R ++
Sbjct: 90  QDLAFSSRSVPNALHAHNQFKFSVVWLPVASRWRSLRKVLNSNIFSGNRLDANQHLRTRK 149

Query: 170 V--------RIESVGTQIDIGKVSFLATMKSV--------IAMTWGDSGR--------VM 229
           V        +    G  +D+G+ +F  ++  +        +   + DS +        +M
Sbjct: 150 VQELIAYCRKNSQSGEAVDVGRAAFRTSLNLLSNLIFSKDLTDPYSDSAKEFKDLVWNIM 209

Query: 230 GEDGE-----------------------------------------EDGRKWWGKGGYLQ 289
            E G+                                         E  R    K   L 
Sbjct: 210 VEAGKPNLVDFFPLLEKVDPQGIRHRMTIHFGEVLKLFGGLVNERLEQRRSKGEKNDVLD 269

Query: 290 LLLELQDNEDNSECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEEL 349
           +L  L  ++++ E I    ++ + LD+ + GT+TTS+T+EWAM+E++++PD MKK ++EL
Sbjct: 270 VL--LTTSQESPEEIDRTHIERMCLDLFVAGTDTTSSTLEWAMSEMLKNPDKMKKTQDEL 329

Query: 350 KEVIGLNAIVEEFHFSKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTI 409
            +VIG    +EE   ++L YL  V+KETLR+HP +  L+PR +  S  + GY +PK S +
Sbjct: 330 AQVIGRGKTIEESDINRLPYLRCVMKETLRIHPPVPFLIPRKVEQSVEVCGYNVPKGSQV 389

Query: 410 YFKLWGIQRDPTIWDNPSKFMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYGKRSCA 456
               W I RD T+WD+   F PERF++S  +    +FEL           PFG G+R C 
Sbjct: 390 LVNAWAIGRDETVWDDALAFKPERFMESELDIRGRDFEL----------IPFGAGRRICP 449

BLAST of Chy9G168470 vs. ExPASy TrEMBL
Match: A0A1S3ATF6 (7-ethoxycoumarin O-deethylase-like OS=Cucumis melo OX=3656 GN=LOC103482655 PE=3 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.1e-219
Identity = 401/535 (74.95%), Postives = 424/535 (79.25%), Query Frame = 0

Query: 1   MSISA-LFFFILPSFWSTHSNENNQISISILLVFLLLFLFWFRPKFWRPCLPPGPCGLPL 60
           MSISA L F ILPSFWST+ NENNQISISILLVFL+L LF FRPKFWRPCLPPGPCGLPL
Sbjct: 32  MSISADLSFSILPSFWSTYFNENNQISISILLVFLVLTLFRFRPKFWRPCLPPGPCGLPL 91

Query: 61  VGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHHQETLFPNRNT 120
           VGYLPFLSGNPHHKLTHLAKIYGPV+KLRLGPKLC+VL SPA +KE FHHQETLFPNR+T
Sbjct: 92  VGYLPFLSGNPHHKLTHLAKIYGPVYKLRLGPKLCIVLNSPASVKEAFHHQETLFPNRDT 151

Query: 121 TVCALLSSSGRSGIVFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVR------IE 180
           TVCALLS  G SGIVF QD  DWKKLRKIF RKMISKSNLDASY VRRQEVR       E
Sbjct: 152 TVCALLSGYGGSGIVFAQDEGDWKKLRKIFTRKMISKSNLDASYFVRRQEVRKVIKGVFE 211

Query: 181 SVGTQIDIGKVSFLATMKSVIAMTWGDSGRVMGEDGE----------------------- 240
           SVGTQIDIGKVSF ATMKSV+AMTWGDSGR+MGEDG                        
Sbjct: 212 SVGTQIDIGKVSFHATMKSVLAMTWGDSGRLMGEDGVGLEEKFREMMDELMVLLGSPNVS 271

Query: 241 ---------------------------------EDGRKWW------GKGGYLQLLLELQD 300
                                            E+ RK        GKGGYLQLLLELQD
Sbjct: 272 DVFPVLGRFDLQGIGRRTKKVMGVLDGILNFSIEEQRKMGGKGNGEGKGGYLQLLLELQD 331

Query: 301 NEDNSECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLN 360
           NEDNSECITNDQLKALLLDIVIGGTETT+TT+EWAMAELMQHP+ +KKVKEELK+VIGLN
Sbjct: 332 NEDNSECITNDQLKALLLDIVIGGTETTATTVEWAMAELMQHPNTIKKVKEELKKVIGLN 391

Query: 361 AIVEEFHFSKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGI 420
           A+VEEFHFSKLCYLNAVIKETLRLHP IFLLVPR+LTSSTTLGGYYIPKDST+YF LW I
Sbjct: 392 AVVEEFHFSKLCYLNAVIKETLRLHPPIFLLVPRSLTSSTTLGGYYIPKDSTLYFNLWAI 451

Query: 421 QRDPTIWDNPSKFMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYGKRSCAGIALAER 467
           QRDP  WDNPSKFMPERF+ +N             N+NAMEFYPFGYGK+SCAG+ALAER
Sbjct: 452 QRDPKTWDNPSKFMPERFINNN-------------NDNAMEFYPFGYGKKSCAGVALAER 511

BLAST of Chy9G168470 vs. ExPASy TrEMBL
Match: A0A5A7THS0 (7-ethoxycoumarin O-deethylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003640 PE=3 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 3.3e-219
Identity = 402/535 (75.14%), Postives = 422/535 (78.88%), Query Frame = 0

Query: 1   MSISA-LFFFILPSFWSTHSNENNQISISILLVFLLLFLFWFRPKFWRPCLPPGPCGLPL 60
           MSISA L F ILPSFWST+ NENNQISISILLVFL+L LF FRPKFWRPCLPPGPCGLPL
Sbjct: 1   MSISADLSFSILPSFWSTYFNENNQISISILLVFLVLTLFRFRPKFWRPCLPPGPCGLPL 60

Query: 61  VGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHHQETLFPNRNT 120
           VGYLPFLSGNPHHKLTHLAKIYGPV+KLRLGPKLC+VL SPA +KE FHHQETLFPNR+T
Sbjct: 61  VGYLPFLSGNPHHKLTHLAKIYGPVYKLRLGPKLCIVLNSPASVKEAFHHQETLFPNRDT 120

Query: 121 TVCALLSSSGRSGIVFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVR------IE 180
           TVCALLS  G SGIVF QD  DWKKLRKIF RKMISKSNLDASY VRRQEVR       E
Sbjct: 121 TVCALLSGYGGSGIVFAQDEGDWKKLRKIFTRKMISKSNLDASYFVRRQEVRKVIKGVFE 180

Query: 181 SVGTQIDIGKVSFLATMKSVIAMTWGDSGRVMGEDGE----------------------- 240
           SVGTQIDIGKVSF ATMKSV+AMTWGDSGR+MGEDG                        
Sbjct: 181 SVGTQIDIGKVSFHATMKSVLAMTWGDSGRLMGEDGVGLEEKFREMMDELMVLLGSPNVS 240

Query: 241 ---------------------------------EDGRKWW------GKGGYLQLLLELQD 300
                                            E+ RK        GKGGYLQLLLELQD
Sbjct: 241 DVFPVLGRFDLQGIGRRTKKVMGVLDGILNFAIEEQRKMGGKGNGEGKGGYLQLLLELQD 300

Query: 301 NEDNSECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLN 360
           NEDNSECITNDQLKALLLDIVIGGTETT+TTIEW MAELMQHP+ +KKVKEELK+VIGLN
Sbjct: 301 NEDNSECITNDQLKALLLDIVIGGTETTATTIEWTMAELMQHPNTIKKVKEELKKVIGLN 360

Query: 361 AIVEEFHFSKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGI 420
           A VEEFHFSKLCYLNAVIKETLRLHP IFLLVPR+LTSSTTLGGYYIPKDST+YF LW I
Sbjct: 361 AAVEEFHFSKLCYLNAVIKETLRLHPPIFLLVPRSLTSSTTLGGYYIPKDSTLYFNLWAI 420

Query: 421 QRDPTIWDNPSKFMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYGKRSCAGIALAER 467
           QRDP  WDNPSKFMPERF+ +N             N+NAMEFYPFGYGKRSCAG+ALAER
Sbjct: 421 QRDPKTWDNPSKFMPERFINNN-------------NDNAMEFYPFGYGKRSCAGVALAER 480

BLAST of Chy9G168470 vs. ExPASy TrEMBL
Match: A0A6J1DW75 (7-ethoxycoumarin O-deethylase-like OS=Momordica charantia OX=3673 GN=LOC111023676 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 5.4e-153
Identity = 302/530 (56.98%), Postives = 358/530 (67.55%), Query Frame = 0

Query: 1   MSISALFFFILPSFWST-HSNENNQISISILLVFLLLF-LFWFR----PKFWRPCLPPGP 60
           +S+  +FFF   SFW+   SNE+ QISI++L +F+L+F LFWFR    PK   P  PP P
Sbjct: 23  VSVPFVFFF---SFWTVDSSNEDKQISIAVLSIFVLIFALFWFRRSGEPKLRLPPSPPSP 82

Query: 61  CGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHHQETLF 120
            GLPL+GYLPFLS N H     LA IY PVFKLRLG KLCVVLTSP+ + E  H Q+T+F
Sbjct: 83  RGLPLLGYLPFLSLNLHRTFADLAGIYSPVFKLRLGTKLCVVLTSPSAVNEALHRQDTVF 142

Query: 121 PNRNTTVCALLSSSGRSGIVFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVR--- 180
            NR+ TVCALL+S G S IV   DG DWKKLRKIF RKM+SKSNL+ASYCVRR+EVR   
Sbjct: 143 ANRDPTVCALLASYGGSDIVITSDGADWKKLRKIFARKMLSKSNLEASYCVRRREVREAI 202

Query: 181 ---IESVGTQIDIGKVSFLATMKSVIAMTWGDSGRVMGEDGEEDGRKWWGK--------- 240
               ES GT ID+G+V FLAT+KSV+AM +G +GR +G D E D R+  G+         
Sbjct: 203 KGVFESAGTPIDVGEVGFLATLKSVMAMIFGGAGRRIGGDLEGDLREKMGELMVLLGTPN 262

Query: 241 --------------------------------------------GGYLQLLLELQDNEDN 300
                                                       GG+LQ LLEL D +D 
Sbjct: 263 VSDIFPALGGFDLQGIGRRTKEVMLMIDEILDLAIEEQKVGENGGGFLQWLLELNDTQDC 322

Query: 301 SECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLNAIVE 360
           S+ IT +QLKALL+DIV+GGT+TTSTTIEWAMAELMQH + M+KV EEL +V+GLN +VE
Sbjct: 323 SDSITTNQLKALLVDIVVGGTDTTSTTIEWAMAELMQHRNIMEKVTEELTQVVGLNQMVE 382

Query: 361 EFHFSKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGIQRDP 420
           EFH SKLC+LNAVIKETLRLHP + LLVPR    ++TLGGY IPK S IYF +W IQRDP
Sbjct: 383 EFHLSKLCFLNAVIKETLRLHPPLPLLVPRIAAQTSTLGGYSIPKGSAIYFNIWAIQRDP 442

Query: 421 TIWDNPSKFMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYGKRSCAGIALAERMLMF 465
            +WDNP KFMP RF+         E E  D   N MEF PFG G+RSC GI LAERML+F
Sbjct: 443 KVWDNPLKFMPRRFL--------NEPEGYDFTGNCMEFCPFGSGRRSCVGIPLAERMLVF 502

BLAST of Chy9G168470 vs. ExPASy TrEMBL
Match: A0A5A7TDF0 (7-ethoxycoumarin O-deethylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00010 PE=3 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 8.6e-151
Identity = 290/521 (55.66%), Postives = 345/521 (66.22%), Query Frame = 0

Query: 11  LPSFWSTHSNENNQISISILLVFLLLF-LFWFRPKFWRPCLPPGPCGLPLVGYLPFLSGN 70
           L SFWS HSN+ NQIS  IL +FL +F L W R KF RP LPPGP GLPLVGYLPFLS N
Sbjct: 3   LISFWSVHSNKLNQISTPILFIFLFIFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSPN 62

Query: 71  PHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHHQETLFPNRNTTVCALLSSSG 130
            HH    LAK+YG + KL LG KLC+VLTSP+ + E   HQET+F  R+TTV ALLSS G
Sbjct: 63  LHHNFADLAKVYGSILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYG 122

Query: 131 RSGIVFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVR------IESVGTQIDIGK 190
            S IVF QD  +WKKLRKIF RKM+SKSNLDASY +RRQEVR       ES GT IDIGK
Sbjct: 123 GSDIVFTQDENNWKKLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGK 182

Query: 191 VSFLATMKSVIAMTWGDSGRVMGEDGE--------------------------------- 250
           + F+A +KSV+AMTWG SG ++G DG                                  
Sbjct: 183 LGFVAALKSVMAMTWGGSGGLIGVDGTDLDAKFREAVDEMMVLLATPNLSDLFPVLGRFD 242

Query: 251 -----------------------EDGRKWWGKG----GYLQLLLELQDNEDNSECITNDQ 310
                                  E+ RK  G G    G+LQ LLE+ + ED+SE IT + 
Sbjct: 243 LQGVVRKMKKVMCVFDEIFNSAIEEQRKRGGNGMENRGFLQFLLEIMEGEDSSESITENN 302

Query: 311 LKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLNAIVEEFHFSKLC 370
           LKALL+DIVIGGT+TTSTT+EWA++EL++ P+ M KV EEL +V+GLN +VEEFH  KL 
Sbjct: 303 LKALLMDIVIGGTDTTSTTVEWAISELIKQPNTMNKVIEELTKVVGLNQMVEEFHLPKLN 362

Query: 371 YLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGIQRDPTIWDNPSK 430
           +L+AV+KETLRLHP + LLVPRT + +TT G Y IPK STIYF +W IQRDP +WDNP  
Sbjct: 363 FLDAVVKETLRLHPPLPLLVPRTSSQTTTFGEYTIPKGSTIYFNIWAIQRDPKVWDNPLN 422

Query: 431 FMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHS 465
           F PERF+  + E       L D   N +EF PFG GK+SCAGI LA+R+L+ ILASLLH 
Sbjct: 423 FEPERFLNESSE------NLYDFTGNRIEFCPFGSGKKSCAGIPLAKRLLVLILASLLHG 482

BLAST of Chy9G168470 vs. ExPASy TrEMBL
Match: A0A1S3ATA1 (7-ethoxycoumarin O-deethylase-like OS=Cucumis melo OX=3656 GN=LOC103482652 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 2.8e-149
Identity = 288/521 (55.28%), Postives = 343/521 (65.83%), Query Frame = 0

Query: 11  LPSFWSTHSNENNQISISILLVFLLLF-LFWFRPKFWRPCLPPGPCGLPLVGYLPFLSGN 70
           L SFWS  SN+ NQIS  IL +FL +F L W R KF RP LPPGP GLPLVGYLPFLS N
Sbjct: 3   LISFWSVDSNKLNQISTPILFIFLFIFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSPN 62

Query: 71  PHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHHQETLFPNRNTTVCALLSSSG 130
            HH    LAK+YG + KL LG KLC+VLTSP+ + E   HQET+F  R+TTV ALLSS G
Sbjct: 63  LHHNFADLAKVYGSILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYG 122

Query: 131 RSGIVFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVR------IESVGTQIDIGK 190
            S IVF QD  +WKKLRKIF RKM+SKSNLDASY +RRQEVR       ES GT IDIGK
Sbjct: 123 GSDIVFTQDENNWKKLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGK 182

Query: 191 VSFLATMKSVIAMTWGDSGRVMGEDGE--------------------------------- 250
           + F+A +KSV+AMTWG SG ++G DG                                  
Sbjct: 183 LGFVAALKSVMAMTWGGSGGLIGVDGTDLDAKFREAVDEMMVLLATPNLSDLFPVLGRFD 242

Query: 251 -----------------------EDGRKWWGKG----GYLQLLLELQDNEDNSECITNDQ 310
                                  E+ RK  G G    G+LQ LLE+ + ED+SE IT + 
Sbjct: 243 LQGVVRKMKKVMCVFDEIFNSAIEEQRKRGGNGMENRGFLQFLLEIMEGEDSSESITENN 302

Query: 311 LKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLNAIVEEFHFSKLC 370
           LKALL+DIVIGGT+TTSTT+EWA++EL++ P+ M KV EEL +V+GLN +VEEFH  KL 
Sbjct: 303 LKALLMDIVIGGTDTTSTTVEWAISELIKQPNTMNKVIEELTKVVGLNQMVEEFHLPKLN 362

Query: 371 YLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGIQRDPTIWDNPSK 430
           +L+AV+KETLRLHP + LLVPRT + +TT G Y IPK STIYF +W IQRDP +WDNP  
Sbjct: 363 FLDAVVKETLRLHPPLPLLVPRTSSQTTTFGEYTIPKGSTIYFNIWAIQRDPKVWDNPLN 422

Query: 431 FMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHS 465
           F PERF+  + E       L D   N +EF PFG GK+SCAGI LA+R+L+ ILASLLH 
Sbjct: 423 FEPERFLNESSE------NLYDFTGNRIEFCPFGSGKKSCAGIPLAKRLLVLILASLLHG 482

BLAST of Chy9G168470 vs. NCBI nr
Match: XP_004147609.1 (flavonoid 3'-monooxygenase CYP75B137 [Cucumis sativus] >KGN49714.2 hypothetical protein Csa_000041 [Cucumis sativus])

HSP 1 Score: 802 bits (2072), Expect = 9.56e-290
Identity = 421/531 (79.28%), Postives = 430/531 (80.98%), Query Frame = 0

Query: 3   ISALFFFILPSFWSTHSNENNQISISILLVFLLLF-LFWFRPKFWRPCLPPGPCGLPLVG 62
           +S L F  L  FWS  S+  NQIS  IL +FL +F L W R KF RP LPPGP GLPLVG
Sbjct: 1   MSLLSFPFLFLFWSVDSDNQNQISTLILFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVG 60

Query: 63  YLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHHQETLFPNRNTTV 122
           YLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKE FHHQETLFPNRNTTV
Sbjct: 61  YLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTV 120

Query: 123 CALLSSSGRSGIVFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVR------IESV 182
           CALLSS G SGIVF QDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVR       ESV
Sbjct: 121 CALLSSYGCSGIVFAQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESV 180

Query: 183 GTQIDIGKVSFLATMKSVIAMTWGDSGRVMGEDGE------------------------- 242
           GTQIDIGKVSFLATMKSV+AMTWGDSGRVMGEDG                          
Sbjct: 181 GTQIDIGKVSFLATMKSVVAMTWGDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDV 240

Query: 243 -------------------------------EDGRKWWG----KGGYLQLLLELQDNEDN 302
                                          E+ RK  G    KGGYLQLLLELQDNEDN
Sbjct: 241 FPVLGRFDLQGIGRRTKKVMGVLDGILNSAIEEQRKMGGNGGGKGGYLQLLLELQDNEDN 300

Query: 303 SECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLNAIVE 362
           SECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPD MKKVKEELK+VIGLNAIVE
Sbjct: 301 SECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVE 360

Query: 363 EFHFSKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGIQRDP 422
           EFHF KLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYF LWGIQRDP
Sbjct: 361 EFHFPKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDP 420

Query: 423 TIWDNPSKFMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYGKRSCAGIALAERMLMF 466
           TIWDNP KFMPERFVKSNEEECAG+FELGDSNN AMEFYPFGYGKRSCAGIALAERMLMF
Sbjct: 421 TIWDNPLKFMPERFVKSNEEECAGQFELGDSNN-AMEFYPFGYGKRSCAGIALAERMLMF 480

BLAST of Chy9G168470 vs. NCBI nr
Match: XP_008437145.1 (PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo])

HSP 1 Score: 771 bits (1991), Expect = 4.76e-277
Identity = 401/535 (74.95%), Postives = 424/535 (79.25%), Query Frame = 0

Query: 1   MSISA-LFFFILPSFWSTHSNENNQISISILLVFLLLFLFWFRPKFWRPCLPPGPCGLPL 60
           MSISA L F ILPSFWST+ NENNQISISILLVFL+L LF FRPKFWRPCLPPGPCGLPL
Sbjct: 32  MSISADLSFSILPSFWSTYFNENNQISISILLVFLVLTLFRFRPKFWRPCLPPGPCGLPL 91

Query: 61  VGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHHQETLFPNRNT 120
           VGYLPFLSGNPHHKLTHLAKIYGPV+KLRLGPKLC+VL SPA +KE FHHQETLFPNR+T
Sbjct: 92  VGYLPFLSGNPHHKLTHLAKIYGPVYKLRLGPKLCIVLNSPASVKEAFHHQETLFPNRDT 151

Query: 121 TVCALLSSSGRSGIVFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVR------IE 180
           TVCALLS  G SGIVF QD  DWKKLRKIF RKMISKSNLDASY VRRQEVR       E
Sbjct: 152 TVCALLSGYGGSGIVFAQDEGDWKKLRKIFTRKMISKSNLDASYFVRRQEVRKVIKGVFE 211

Query: 181 SVGTQIDIGKVSFLATMKSVIAMTWGDSGRVMGEDGE----------------------- 240
           SVGTQIDIGKVSF ATMKSV+AMTWGDSGR+MGEDG                        
Sbjct: 212 SVGTQIDIGKVSFHATMKSVLAMTWGDSGRLMGEDGVGLEEKFREMMDELMVLLGSPNVS 271

Query: 241 ---------------------------------EDGRKWWGKG------GYLQLLLELQD 300
                                            E+ RK  GKG      GYLQLLLELQD
Sbjct: 272 DVFPVLGRFDLQGIGRRTKKVMGVLDGILNFSIEEQRKMGGKGNGEGKGGYLQLLLELQD 331

Query: 301 NEDNSECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLN 360
           NEDNSECITNDQLKALLLDIVIGGTETT+TT+EWAMAELMQHP+ +KKVKEELK+VIGLN
Sbjct: 332 NEDNSECITNDQLKALLLDIVIGGTETTATTVEWAMAELMQHPNTIKKVKEELKKVIGLN 391

Query: 361 AIVEEFHFSKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGI 420
           A+VEEFHFSKLCYLNAVIKETLRLHP IFLLVPR+LTSSTTLGGYYIPKDST+YF LW I
Sbjct: 392 AVVEEFHFSKLCYLNAVIKETLRLHPPIFLLVPRSLTSSTTLGGYYIPKDSTLYFNLWAI 451

Query: 421 QRDPTIWDNPSKFMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYGKRSCAGIALAER 466
           QRDP  WDNPSKFMPERF+ +N             N+NAMEFYPFGYGK+SCAG+ALAER
Sbjct: 452 QRDPKTWDNPSKFMPERFINNN-------------NDNAMEFYPFGYGKKSCAGVALAER 511

BLAST of Chy9G168470 vs. NCBI nr
Match: KAA0042853.1 (7-ethoxycoumarin O-deethylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 769 bits (1987), Expect = 6.18e-277
Identity = 402/535 (75.14%), Postives = 422/535 (78.88%), Query Frame = 0

Query: 1   MSISA-LFFFILPSFWSTHSNENNQISISILLVFLLLFLFWFRPKFWRPCLPPGPCGLPL 60
           MSISA L F ILPSFWST+ NENNQISISILLVFL+L LF FRPKFWRPCLPPGPCGLPL
Sbjct: 1   MSISADLSFSILPSFWSTYFNENNQISISILLVFLVLTLFRFRPKFWRPCLPPGPCGLPL 60

Query: 61  VGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHHQETLFPNRNT 120
           VGYLPFLSGNPHHKLTHLAKIYGPV+KLRLGPKLC+VL SPA +KE FHHQETLFPNR+T
Sbjct: 61  VGYLPFLSGNPHHKLTHLAKIYGPVYKLRLGPKLCIVLNSPASVKEAFHHQETLFPNRDT 120

Query: 121 TVCALLSSSGRSGIVFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVR------IE 180
           TVCALLS  G SGIVF QD  DWKKLRKIF RKMISKSNLDASY VRRQEVR       E
Sbjct: 121 TVCALLSGYGGSGIVFAQDEGDWKKLRKIFTRKMISKSNLDASYFVRRQEVRKVIKGVFE 180

Query: 181 SVGTQIDIGKVSFLATMKSVIAMTWGDSGRVMGEDGE----------------------- 240
           SVGTQIDIGKVSF ATMKSV+AMTWGDSGR+MGEDG                        
Sbjct: 181 SVGTQIDIGKVSFHATMKSVLAMTWGDSGRLMGEDGVGLEEKFREMMDELMVLLGSPNVS 240

Query: 241 ---------------------------------EDGRKWWGKG------GYLQLLLELQD 300
                                            E+ RK  GKG      GYLQLLLELQD
Sbjct: 241 DVFPVLGRFDLQGIGRRTKKVMGVLDGILNFAIEEQRKMGGKGNGEGKGGYLQLLLELQD 300

Query: 301 NEDNSECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLN 360
           NEDNSECITNDQLKALLLDIVIGGTETT+TTIEW MAELMQHP+ +KKVKEELK+VIGLN
Sbjct: 301 NEDNSECITNDQLKALLLDIVIGGTETTATTIEWTMAELMQHPNTIKKVKEELKKVIGLN 360

Query: 361 AIVEEFHFSKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGI 420
           A VEEFHFSKLCYLNAVIKETLRLHP IFLLVPR+LTSSTTLGGYYIPKDST+YF LW I
Sbjct: 361 AAVEEFHFSKLCYLNAVIKETLRLHPPIFLLVPRSLTSSTTLGGYYIPKDSTLYFNLWAI 420

Query: 421 QRDPTIWDNPSKFMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYGKRSCAGIALAER 466
           QRDP  WDNPSKFMPERF+ +N             N+NAMEFYPFGYGKRSCAG+ALAER
Sbjct: 421 QRDPKTWDNPSKFMPERFINNN-------------NDNAMEFYPFGYGKRSCAGVALAER 480

BLAST of Chy9G168470 vs. NCBI nr
Match: XP_038875602.1 (labd-13Z-ene-9,15,16-triol synthase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 682 bits (1759), Expect = 3.48e-242
Identity = 364/533 (68.29%), Postives = 392/533 (73.55%), Query Frame = 0

Query: 1   MSISALFFFILPSFWSTHSNENNQISISILLVFLLLFLFWFRPKFWRPCLPPGPCGLPLV 60
           MSISA F F L SFWS  SNE+ Q+S SILLV L   LFWFRPK  RP LPPGP GLPLV
Sbjct: 1   MSISAPFSFFLTSFWSVDSNEDKQLSFSILLVVLTFALFWFRPKLRRPSLPPGPRGLPLV 60

Query: 61  GYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHHQETLFPNRNTT 120
           GYLPFLS NPHHKLTHLA IYGPVFKLRLG KL +VLTSP  IKE F HQE L PNR+T+
Sbjct: 61  GYLPFLSANPHHKLTHLADIYGPVFKLRLGTKLSIVLTSPDSIKEAFRHQEALLPNRDTS 120

Query: 121 VCALLSSSGRSGIVFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVR------IES 180
           VCA LSS G SGIVF QD  DWKKLRKIFVRKM+SKSNLDASY VRRQEVR       E+
Sbjct: 121 VCAFLSSYGGSGIVFTQDDGDWKKLRKIFVRKMLSKSNLDASYSVRRQEVRKVIKGVFET 180

Query: 181 VGTQIDIGKVSFLATMKSVIAMTWGDSGRVMGEDGE------------------------ 240
             TQI+I KV FLA +KSV+AMTWGDSGR++GEDG                         
Sbjct: 181 ARTQIEIRKVGFLAALKSVMAMTWGDSGRLIGEDGVDLEVKFREVMDELVVLLGTPNVSD 240

Query: 241 --------------------------------EDGRKWWG----KGGYLQLLLELQDNED 300
                                           E+ RK  G    KGGYLQ+LLELQDNED
Sbjct: 241 FFPVLGRFDLQGIAKRTKKVMRACDEILNSAIEEQRKMGGNGVEKGGYLQMLLELQDNED 300

Query: 301 NSECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLNAIV 360
           +SECIT+DQLKALLLDIVIGGTETT+TTIEWAMAELMQHP+ MKKVKEELKEVIGLNA+ 
Sbjct: 301 SSECITDDQLKALLLDIVIGGTETTATTIEWAMAELMQHPNTMKKVKEELKEVIGLNAVA 360

Query: 361 EEFHFSKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGIQRD 420
           EEFHFSKLCYLNAVIKETLRLHP IF LVPR LTS+TT GGY IP DSTIYF +W IQRD
Sbjct: 361 EEFHFSKLCYLNAVIKETLRLHPPIFFLVPRILTSTTTFGGYSIPTDSTIYFNIWAIQRD 420

Query: 421 PTIWDNPSKFMPERFVKSNEEECAGEFE-LGDSNNNAMEFYPFGYGKRSCAGIALAERML 466
              W+NP  FMPERF+ +    CA   E +GDS   AMEF PFGYGKRSCAGI LAERML
Sbjct: 421 SKAWNNPLNFMPERFLNN----CAENDEFIGDS---AMEFCPFGYGKRSCAGIPLAERML 480

BLAST of Chy9G168470 vs. NCBI nr
Match: XP_023550541.1 (ferruginol synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 608 bits (1568), Expect = 3.20e-213
Identity = 333/532 (62.59%), Postives = 374/532 (70.30%), Query Frame = 0

Query: 1   MSISALFFFILPSFWSTHSNENNQISISILLVFLLLF-LFWFRPKFWRPCLPPGPCGLPL 60
           MSISA     L SFWS  S+E  QIS +I LV ++ F LFWFR K  RP LPPGP GLPL
Sbjct: 1   MSISAF----LCSFWSLDSDEVKQISTAIFLVSVVTFALFWFRSKVRRPSLPPGPRGLPL 60

Query: 61  VGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHHQETLFPNRNT 120
           VGYLPFL  N H     LA+IYG VFKLR+G KLCVVL SP+ I E F H ET+FPNR+T
Sbjct: 61  VGYLPFLLRNIHRTFADLAEIYGAVFKLRIGNKLCVVLNSPSSINEAFRHHETVFPNRDT 120

Query: 121 TVCALLSSSGRSGIVFGQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVR------IE 180
           TVCALL S   SGIVF QDG+DWKKLRKIFVRKM+SKS LDASY VRRQEVR       E
Sbjct: 121 TVCALLCSYDGSGIVFTQDGDDWKKLRKIFVRKMLSKSFLDASYSVRRQEVRKVIKGVFE 180

Query: 181 SVGTQIDIGKVSFLATMKSVIAMTWGDSGRVMGEDGE----------------------- 240
           S GT IDIGKV FLATMKSV+AMTWGDSGR++GEDG                        
Sbjct: 181 SAGTPIDIGKVGFLATMKSVLAMTWGDSGRLIGEDGIDLDVKFRAVMDELAVLLGTPNLS 240

Query: 241 ---------------------------------EDGRKWWG----KGGYLQLLLELQDNE 300
                                            E+ RK  G    KGGYLQLLLEL+D++
Sbjct: 241 DIFPILGWFDLQGIASRTKKAMRVCDEILNTAIEEQRKTGGNGVVKGGYLQLLLELEDDQ 300

Query: 301 DNSECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLNAI 360
           DNS  IT+DQLKALLLD+VIGGT+TT TTIEWAMAELMQHP+ MKKVKEEL+EV+GL+A 
Sbjct: 301 DNSNPITDDQLKALLLDMVIGGTKTTVTTIEWAMAELMQHPNTMKKVKEELREVVGLDAA 360

Query: 361 VEEFHFSKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGIQR 420
            EE H  KL +LNAV+KET+RLHP IFLLVPR+LTS+ TL GY IPK S +Y  +W I R
Sbjct: 361 AEESHMPKLSFLNAVVKETIRLHPPIFLLVPRSLTSTITLEGYSIPKGSAMYCNIWAILR 420

Query: 421 DPTIWDNPSKFMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYGKRSCAGIALAERML 465
           DP +W+NP KFMPERF+     E  G++ELG S   AMEF PFGYGKRSC GI LAERML
Sbjct: 421 DPKVWENPLKFMPERFLN----EGGGKYELGGS---AMEFSPFGYGKRSCPGIPLAERML 480

BLAST of Chy9G168470 vs. TAIR 10
Match: AT4G12300.1 (cytochrome P450, family 706, subfamily A, polypeptide 4 )

HSP 1 Score: 350.9 bits (899), Expect = 1.6e-96
Identity = 195/505 (38.61%), Postives = 290/505 (57.43%), Query Frame = 0

Query: 29  ILLVFLLLFLFWFRPKFWRPCLPPGPCGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLR 88
           ++L  +   L++   +  +P LPPGP GLP+VG LPFL  + H    +LA+ +GP+FKL 
Sbjct: 21  VILTTVFSILWYIFKRSPQPSLPPGPRGLPIVGNLPFLDPDLHTYFANLAQSHGPIFKLN 80

Query: 89  LGPKLCVVLTSPAFIKEVFHHQETLFPNRNTTVCALLSSSGRSGIVFGQDGEDWKKLRKI 148
           LG KL +V+ SP+  +E+   Q+  F NR+  +    ++ G   IV+   G +W++LRKI
Sbjct: 81  LGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAATYGGIDIVWTPYGAEWRQLRKI 140

Query: 149 FVRKMISKSNLDASYCVRRQEVRIES--------VGTQIDIGKVSFLATMKSVIAMTWGD 208
            V K++S+  LD+ Y +RR+EVR  +          + + +G   FL  M   + M WG 
Sbjct: 141 CVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSPVKVGDQLFLTMMNLTMNMLWGG 200

Query: 209 S--------------------GRVMGEDGEEDGRKWWGK--------------------- 268
           S                     R++ E    D   W  +                     
Sbjct: 201 SVKAEEMESVGTEFKGVISEITRLLSEPNVSDFFPWLARFDLQGLVKRMGVCARELDAVL 260

Query: 269 ------------------GGYLQLLLELQDNEDNSEC-ITNDQLKALLLDIVIGGTETTS 328
                               +LQ L++L+D E +SE  IT + +KALL D+V+GGT+T++
Sbjct: 261 DRAIEQMKPLRGRDDDEVKDFLQYLMKLKDQEGDSEVPITINHVKALLTDMVVGGTDTST 320

Query: 329 TTIEWAMAELMQHPDAMKKVKEELKEVIGLNAIVEEFHFSKLCYLNAVIKETLRLHPAIF 388
            TIE+AMAELM +P+ +K+ +EEL EV+G + IVEE H ++L Y+ A++KETLRLHP + 
Sbjct: 321 NTIEFAMAELMSNPELIKRAQEELDEVVGKDNIVEESHITRLPYILAIMKETLRLHPTLP 380

Query: 389 LLVPRTLTSSTTLGGYYIPKDSTIYFKLWGIQRDPTIWDNPSKFMPERFVKSNEEECAGE 448
           LLVP     +T +GGY IPKD+ I+  +W IQRDP +W+NP++F PERF+ +N  +  G 
Sbjct: 381 LLVPHRPAENTVVGGYTIPKDTKIFVNVWSIQRDPNVWENPTEFRPERFLDNNSCDFTGA 440

Query: 449 FELGDSNNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWELPKDSVIDFKEKF 466
                       ++PFG G+R CAG+ALAERM+++ LA+LLHSF+W++P+  V+D KEKF
Sbjct: 441 ---------NYSYFPFGSGRRICAGVALAERMVLYTLATLLHSFDWKIPEGHVLDLKEKF 500

BLAST of Chy9G168470 vs. TAIR 10
Match: AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6 )

HSP 1 Score: 340.1 bits (871), Expect = 2.8e-93
Identity = 194/517 (37.52%), Postives = 298/517 (57.64%), Query Frame = 0

Query: 20  NENNQISISILLVFLLLFLFWFR-PKFWRPCLPPGPCGLPLVGYLPFLSGNPHHKLTHLA 79
           N  N    +IL++  +  + W+   +  +P LPPGP GLP+VG LPFL  + H   T LA
Sbjct: 11  NTINLTPYAILILIAIFSILWYLFKRSPQPHLPPGPRGLPIVGNLPFLDPDLHTYFTKLA 70

Query: 80  KIYGPVFKLRLGPKLCVVLTSPAFIKEVFHHQETLFPNRNTTVCALLSSSGRSGIVFGQD 139
           + YGP+FKL LG KL VV+ +P+  +E+   Q+  F N +  + A   + G   +V+   
Sbjct: 71  ESYGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWLPY 130

Query: 140 GEDWKKLRKIFVRKMISKSNLDASYCVRRQEVRIES--------VGTQIDIGKVSFLATM 199
           G +W+ LRK+ V K++S   L++ Y +RR+E+R  +          + +++G+  FL  M
Sbjct: 131 GAEWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLTMM 190

Query: 200 KSVIAMTWGDS--------------------GRVMGEDGEEDG----------------- 259
              + M WG S                     R++GE    D                  
Sbjct: 191 NLTMNMLWGGSVKAEEMESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGLVKKMH 250

Query: 260 --------------------RKWWGKGG----YLQLLLELQDNEDNSEC-ITNDQLKALL 319
                               R   G  G    +LQ L++L+D E +SE  IT + +KA+L
Sbjct: 251 VCARELDAILDRAIEQMQLLRTRDGDDGECKDFLQHLMKLKDQEADSEVPITVNHVKAVL 310

Query: 320 LDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLNAIVEEFHFSKLCYLNAV 379
           +D+V+GGT+T++ TIE+AMAEL++ P+ MK+ ++EL EV+G + I+EE H ++L +++A+
Sbjct: 311 VDLVVGGTDTSTNTIEFAMAELIRKPELMKRAQQELDEVVGKDNIIEESHITRLPFISAI 370

Query: 380 IKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGIQRDPTIWDNPSKFMPER 439
           +KETLRL+P I LLVP   + +  +GGY IPK++ I+  +W IQRDP +W+ P++F PER
Sbjct: 371 MKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPER 430

Query: 440 FVKSNEEECAGEFELGDSNNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWEL 466
           F+  +++ C       D       + PFG G+R CAGIALAERM+++ LA+LLHSF+W++
Sbjct: 431 FL--DKKSC-------DFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWKI 490

BLAST of Chy9G168470 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 327.8 bits (839), Expect = 1.4e-89
Identity = 189/508 (37.20%), Postives = 285/508 (56.10%), Query Frame = 0

Query: 29  ILLVFLLLFLFWFRPKFWRPCLPPGPCGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLR 88
           ++L      L++   +  +P LPPGP GLP+VG LPFL  + H   T LA+ +GP+FKL 
Sbjct: 23  LILTATFSILWYIFKRSPQPPLPPGPRGLPIVGNLPFLDPDLHTYFTKLAQSHGPIFKLN 82

Query: 89  LGPKLCVVLTSPAFIKEVFHHQETLFPNRNTTVCALLSSSGRSGIVFGQDGEDWKKLRKI 148
           LG KL VV+ SP+   E+   Q+  F N +  + A   + G   +V+   G +W+ LRK+
Sbjct: 83  LGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRMLRKV 142

Query: 149 FVRKMISKSNLDASYCVRRQEVRIES--------VGTQIDIGKVSFLATMKSVIAMTWGD 208
              K+ S+  LD+ Y +RR+E+R  +          + +++G+  FL  M  ++ M WG 
Sbjct: 143 CAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMNLMMNMLWG- 202

Query: 209 SGRVMGEDGEEDGRKWWG------------------------------------------ 268
            G V  ED E  G ++ G                                          
Sbjct: 203 -GSVKAEDMESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVKKMHLYARDLDA 262

Query: 269 --------------KGG-------YLQLLLELQDNEDNSEC-ITNDQLKALLLDIVIGGT 328
                         + G       +LQ L++L+D E +S+  IT + +KA+L+D+V+GGT
Sbjct: 263 ILDRAIEQMQRLRSRDGDDGECKDFLQHLMKLRDQEADSDVPITMNHVKAVLMDMVVGGT 322

Query: 329 ETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLNAIVEEFHFSKLCYLNAVIKETLRLH 388
           E+++ TIE+ MAEL+ +P+ M++ ++EL EV+G + IVEE H + L Y+ AV+KETLRL+
Sbjct: 323 ESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITSLPYILAVLKETLRLY 382

Query: 389 PAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGIQRDPTIWDNPSKFMPERFVKSNEEE 448
           P I LLVP   + +  +GGY IPK++ I+  +W IQRDP +W+ P++F PERF+  +++ 
Sbjct: 383 PTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPERFL--DKKS 442

Query: 449 CAGEFELGDSNNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWELPKDSVIDF 465
           C       D       + PFG G+R CAGIALAERM+++ LA+LLHSF+W +P   V+D 
Sbjct: 443 C-------DFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWTIPDGHVLDL 502

BLAST of Chy9G168470 vs. TAIR 10
Match: AT4G12330.1 (cytochrome P450, family 706, subfamily A, polypeptide 7 )

HSP 1 Score: 310.5 bits (794), Expect = 2.4e-84
Identity = 182/525 (34.67%), Postives = 284/525 (54.10%), Query Frame = 0

Query: 17  THSNENNQISISILLVFLLLFLFWF-----RPKFWRPCLPPGPCGLPLVGYLPFLSGNPH 76
           T  N N+     +L   ++  +FW+     + K   P LPPGP GLP+VG LPFL    H
Sbjct: 9   TIQNRNDHFDF-VLATIVISSVFWYIWVYVKSKRLFPPLPPGPRGLPIVGNLPFLHPELH 68

Query: 77  HKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEVFHHQETLFPNRNTTVCALLSSSGRS 136
                LA+ +GPVFKL LG KL +V+TS    +++    + +F N +  V   LS+ G  
Sbjct: 69  TYFHSLAQKHGPVFKLWLGAKLTIVITSSEATRDILRTNDVIFANDDVPVAGSLSTYGGV 128

Query: 137 GIVFGQDGEDWKKLRKIFVRKMISKSNLDAS--YCVRRQEVR--------IESVGTQIDI 196
            IV+   G +W  LRKI + KM+S + LD++    +RRQE R            G  +++
Sbjct: 129 DIVWSPYGPEWPMLRKICINKMLSNATLDSNSFSALRRQETRRTVRYLADRARAGLAVNV 188

Query: 197 GKVSFLATMKSVIAMTWGDS---------------------------------------- 256
           G+  F+  +  V  M WG++                                        
Sbjct: 189 GEQIFVTILNVVTQMLWGETVADDEEREKVGAEFLELITEIIDVVGKPNVSDFFPVLSRF 248

Query: 257 --------------------GRVMGED-GEEDGRKWWGKGGYLQLLLELQDNEDNSECIT 316
                                R++ +  G + G K  G G +L +LL  +D ++N   ++
Sbjct: 249 DLQGLAKRVRRSAQRMDRMFDRIISQRMGMDKGSKGNG-GDFLMVLLNAKDEDEN---MS 308

Query: 317 NDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLNAIVEEFHFS 376
            + +KALL+D+V+GGT+T+  TIE+AMAEL+   + MK+ ++EL +V+G N IVEE H +
Sbjct: 309 MNHVKALLMDMVLGGTDTSLNTIEFAMAELINKLEIMKRAQQELDKVVGKNNIVEEKHIT 368

Query: 377 KLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGIQRDPTIWDN 436
           KL Y+ +++KETLRLHPA+ LL+PR  + +T +GGY IP DS ++  +W I R+P +W+N
Sbjct: 369 KLPYILSIMKETLRLHPALPLLIPRCPSETTVIGGYTIPNDSKVFINVWAIHRNPNVWEN 428

Query: 437 PSKFMPERFVKSNEEECAGEFELGDSNNNAMEFYPFGYGKRSCAGIALAERMLMFILASL 466
           P +F P+RF+              D + N   ++PFG G+R CAG+A+AE+++++ LA+L
Sbjct: 429 PLEFNPDRFLDKGY----------DFSGNDYSYFPFGSGRRICAGMAMAEKVVLYNLATL 488

BLAST of Chy9G168470 vs. TAIR 10
Match: AT5G44620.1 (cytochrome P450, family 706, subfamily A, polypeptide 3 )

HSP 1 Score: 303.9 bits (777), Expect = 2.2e-82
Identity = 174/513 (33.92%), Postives = 273/513 (53.22%), Query Frame = 0

Query: 27  ISILLVFLLLFLFWFRPKFWR--PCLPPGPCGLPLVGYLPFLSGNPHHKLTHLAKIYGPV 86
           ++++++  L +  W   K  R  P LPPGP GLP++G LPFL    H     LAK +GP+
Sbjct: 21  LTVIVISTLCWCLWLYAKCKRRSPPLPPGPWGLPIIGNLPFLQPELHTYFQGLAKKHGPI 80

Query: 87  FKLRLGPKLCVVLTSPAFIKEVFHHQETLFPNRNTTVCALLSSSGRSGIVFGQDGEDWKK 146
           FKL LG KL +V+TS    +E+    + +F N +      +++ G + I++   G  W+ 
Sbjct: 81  FKLWLGAKLTIVVTSSEVAQEILKTNDIIFANHDVPAVGPVNTYGGTEIIWSPYGPKWRM 140

Query: 147 LRKIFVRKMISKSNLDASYCVRRQEVR--------IESVGTQIDIGKVSFLATMKSVIAM 206
           LRK+ V +++  + LD+S  +RR+E R           VG+ +++G+  FL  +  V  M
Sbjct: 141 LRKLCVNRILRNAMLDSSTDLRRRETRQTVRYLADQARVGSPVNLGEQIFLMMLNVVTQM 200

Query: 207 TWG-----DSGRVMGED------------------------------------------- 266
            WG     +   V+G +                                           
Sbjct: 201 LWGTTVKEEEREVVGAEFLEVIREMNDLLLVPNISDFFPVLSRFDLQGLAKRMRRPAQRM 260

Query: 267 ----------------GEEDGRKWWGKGGYLQLLLELQDNEDNSECITNDQLKALLLDIV 326
                              DGR       +L +LL+++D E     +T + +KA+L+D+V
Sbjct: 261 DQMFDRIINQRLGMDRDSSDGR----AVDFLDVLLKVKDEEAEKTKLTMNDVKAVLMDMV 320

Query: 327 IGGTETTSTTIEWAMAELMQHPDAMKKVKEELKEVIGLNAIVEEFHFSKLCYLNAVIKET 386
           +GGT+T+   IE+AMAEL+ +PD MK+ ++E+ +V+G   +VEE H SKL Y+ A++KET
Sbjct: 321 LGGTDTSLHVIEFAMAELLHNPDIMKRAQQEVDKVVGKEKVVEESHISKLPYILAIMKET 380

Query: 387 LRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFKLWGIQRDPTIWDNPSKFMPERFVKS 446
           LRLH    LLVPR  + +T +GG+ IPKDS I+   W I R+P +W+NP KF P+RF+  
Sbjct: 381 LRLHTVAPLLVPRRPSQTTVVGGFTIPKDSKIFINAWAIHRNPNVWENPLKFDPDRFLDM 440

Query: 447 NEEECAGEFELGDSNNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWELPKDS 466
           +           D   N   + PFG G+R C G+A+ ER++++ LA+ LHSF+W++P+  
Sbjct: 441 SY----------DFKGNDFNYLPFGSGRRICVGMAMGERVVLYNLATFLHSFDWKIPQGE 500

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A0S1TPC78.2e-6633.26Carnosic acid synthase OS=Salvia pomifera OX=396869 GN=CYP76AK6 PE=1 SV=1[more]
D1MI461.0e-6333.47Geraniol 8-hydroxylase OS=Swertia mussotii OX=137888 GN=CYP76B10 PE=1 SV=1[more]
A0A4D6Q4152.2e-6331.94Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN... [more]
A0A1D8QMD15.0e-6332.01Carnosic acid synthase OS=Salvia fruticosa OX=268906 GN=CYP76AK6 PE=1 SV=1[more]
Q8VWZ75.5e-6231.30Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3ATF61.1e-21974.957-ethoxycoumarin O-deethylase-like OS=Cucumis melo OX=3656 GN=LOC103482655 PE=3 ... [more]
A0A5A7THS03.3e-21975.147-ethoxycoumarin O-deethylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A6J1DW755.4e-15356.987-ethoxycoumarin O-deethylase-like OS=Momordica charantia OX=3673 GN=LOC11102367... [more]
A0A5A7TDF08.6e-15155.667-ethoxycoumarin O-deethylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3ATA12.8e-14955.287-ethoxycoumarin O-deethylase-like OS=Cucumis melo OX=3656 GN=LOC103482652 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_004147609.19.56e-29079.28flavonoid 3'-monooxygenase CYP75B137 [Cucumis sativus] >KGN49714.2 hypothetical ... [more]
XP_008437145.14.76e-27774.95PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo][more]
KAA0042853.16.18e-27775.147-ethoxycoumarin O-deethylase-like [Cucumis melo var. makuwa][more]
XP_038875602.13.48e-24268.29labd-13Z-ene-9,15,16-triol synthase, chloroplastic-like [Benincasa hispida][more]
XP_023550541.13.20e-21362.59ferruginol synthase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G12300.11.6e-9638.61cytochrome P450, family 706, subfamily A, polypeptide 4 [more]
AT4G12320.12.8e-9337.52cytochrome P450, family 706, subfamily A, polypeptide 6 [more]
AT4G12310.11.4e-8937.20cytochrome P450, family 706, subfamily A, polypeptide 5 [more]
AT4G12330.12.4e-8434.67cytochrome P450, family 706, subfamily A, polypeptide 7 [more]
AT5G44620.12.2e-8233.92cytochrome P450, family 706, subfamily A, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 78..97
score: 44.38
coord: 306..324
score: 39.58
coord: 347..371
score: 36.6
coord: 403..426
score: 34.19
IPR001128Cytochrome P450PRINTSPR00385P450coord: 307..318
score: 51.43
coord: 403..414
score: 35.27
coord: 394..403
score: 57.92
coord: 254..271
score: 40.12
IPR001128Cytochrome P450PFAMPF00067p450coord: 51..159
e-value: 6.2E-17
score: 61.5
coord: 223..448
e-value: 7.4E-68
score: 229.4
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 46..210
e-value: 2.0E-23
score: 84.8
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 211..451
e-value: 5.3E-71
score: 241.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 51..456
NoneNo IPR availablePANTHERPTHR47951OS08G0547900 PROTEINcoord: 17..202
coord: 213..464
NoneNo IPR availablePANTHERPTHR47951:SF2P450, PUTATIVE-RELATEDcoord: 17..202
NoneNo IPR availablePANTHERPTHR47951:SF2P450, PUTATIVE-RELATEDcoord: 213..464
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 396..405

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy9G168470.1Chy9G168470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen