Chy9G166410 (gene) Cucumber (hystrix) v1

Overview
NameChy9G166410
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionCytochrome P450 76A2
LocationchrH09: 12052701 .. 12054908 (-)
RNA-Seq ExpressionChy9G166410
SyntenyChy9G166410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCCCTGATAGAGCTCTCCACGATCAGCTCTCTCTTGTTTTCTCCCCTCAATGTGGGGACCAATGGAAGAATTTGAAGTCAATTTGTAAAGTGAACTTGTTTACAGCTAAAGAAATTGAATCACAGGCTATTTTGAGAGAGAAGAAGATGCAGGAGTTAGTGTAATTTGTGGAATCAAAACAGGGATCTATTGTGGAGATTAAAGATTTTGTTTTTACCTCTGTTTTCAATACACTTTCTAACTTGATTTTATCGATGGATTTGCTTGATTTTGTTGGTGATGGGTTTAACGGGATTAAAGGGCCTTTCAAGAAAATGATGAATTTGGGTTTGACTCCAAATTTGGCTGATTTTTATCCATTGTTGAGAAGGTTTGATCTTCAAGGTCTTAGAAAGAAAGCTGCCATTTACATGAATGAAGTTCATTCTTTGTGGGGGATTCTTATTAAAGAAAGAAGAGAAATTCATTGTAAAAAAGGTTCAGCTTCAAGTGATTTCTTGGATGTCTTGATTCAAAGTGGATTTAGTGATCAACAAATCAATGCCTTGATTGTTGTAAGTACAGTTAAATCACTCTTTTGACCACTTTGAAATACCATGCTAGTCTTCGTCATTTTTTTGACGTCGAGTCGGACATCTTTCAAGAATATGTTTACTCTAATTTCTTTAAGTGAAGATTGGTTAAACTTGAGTTTTTTTTAGACAACTCGATATGTGATGTGTTAGTGTCGAATGACTACTTAGATTGTGTCATTTTTTGAATTAGCGAAATTTTTGGAATCAAAACAAAGAAATGTTGTCGAATCTCTTTTGAGTTTTTTTTAGACAACTCGATATGTGAATTTTGAAAACAATGAAAATAAACATTTTTGTTTATGTCACATACTACTTAGATTGTGTTTGATATTTTCTTTGTTCCTCTCACTTGCCCAACCTCCTTTCCTTCCTTTTACTTCCACTTGTGCTTATCGTTACTGTTTGACTTTTATCACCCATTTCAACAAATCTTGTGCTTGAGCTAAGGCAACCGAGGAATCACTCATCACTCACTTTGATAAACTCTCATGTTTGTTTAATCGTGAGTGAGCGGTTTTGAGAGAGGAACACAAGAAGTTGTTTTAATGTAAGGAAAGAGAAACATGAACGCGAGGATTTGGGAGGGAGAAGCTCGGGTGAGAGGTACATAGAAAGAAGTAAGTGCGATTAAGTGGTATGTGAGAAAGAAAAAAATATTAATATATTCATTGTTCTCAAAATCCAAGTTCACTTTGATAAACTTGAAAAATAAATCAAGTCATTCATGAAAAAAAAAATTGAAAAAAAGACGTTGAACTTCATGGACACATGTTAGAGGATTTACACAATGAAAAAATGGGCAGGTTTCACATTCTTTATAATATATATTTCAGTTATTACTCTTCTTATTTTCAATTAAACTCACATATATCTGATACAAAATTACAGGAAGCCAATAAGTCTCCAACTTTTGGTGCATATTATGATGATAGTGAGATCTCTTCATTTTGAGACAGATAGATTTATAATCAAATATATCTAATGCATTATTAATTATTACTACTTTTGCAGGAATTGCTATCTGCAGGCACAGACACTACTACAACAACAGTGGAATGGGCAATGTCAGAGATTCTAAAAGACAAAGACATACTAAACAAAGTAAGAGAGGAAATAAAGAAGGTAACAAAGGAAAACCCCATTGATGAATCCAACATTTCTAAGCTTCAATATCTATGGAGTTGTGTGAAAGAAACCCTAAGACTACACCCACCTGCCCCATTTCTTCTTCCACGTTTAGCTCCAACGGACTGTGAAGTTATGGGATACTCAATCCCAAAAGACAGTATGATTTTTGTCAATGTTTGGGGAATTGGGAGAGACCCTTCAATTTGGGAAGATTCTCAAACGTTCAATCCTCAAAGATTTGATGTTGGTTGTGGTAATAATGTGGATTTTAAAGGGTATGATTATAGGTATTTGCCATTTGGTGGAGGAAGAAGAATTTGCCCTGGTCTGCCAATGGCCATTGTGCAAGTGCCTTTGATTTTAGCCACATTAATTCACAACTTTGAATGGTCACTTCCTAATGGTGAAGATTTATCAAAGTTGGATTTGAATGGAAGGCTTGGTGTAACATTGCAAAAGTATGAAACTCTTAAACTTATTCCTAAAAGGAGGGTTATTTGA

mRNA sequence

ATGACCCCTGATAGAGCTCTCCACGATCAGCTCTCTCTTGTTTTCTCCCCTCAATGTGGGGACCAATGGAAGAATTTGAAGTCAATTTGTAAAGGATCTATTGTGGAGATTAAAGATTTTGTTTTTACCTCTGTTTTCAATACACTTTCTAACTTGATTTTATCGATGGATTTGCTTGATTTTGTTGGTGATGGGTTTAACGGGATTAAAGGGCCTTTCAAGAAAATGATGAATTTGGGTTTGACTCCAAATTTGGCTGATTTTTATCCATTGTTGAGAAGGTTTGATCTTCAAGGTCTTAGAAAGAAAGCTGCCATTTACATGAATGAAGTTCATTCTTTGTGGGGGATTCTTATTAAAGAAAGAAGAGAAATTCATTGTAAAAAAGGTTCAGCTTCAAGTGATTTCTTGGATGTCTTGATTCAAAGTGGATTTAGTGATCAACAAATCAATGCCTTGATTGTTGAATTGCTATCTGCAGGCACAGACACTACTACAACAACAGTGGAATGGGCAATGTCAGAGATTCTAAAAGACAAAGACATACTAAACAAAGTAAGAGAGGAAATAAAGAAGGTAACAAAGGAAAACCCCATTGATGAATCCAACATTTCTAAGCTTCAATATCTATGGAGTTGTGTGAAAGAAACCCTAAGACTACACCCACCTGCCCCATTTCTTCTTCCACGTTTAGCTCCAACGGACTGTGAAGTTATGGGATACTCAATCCCAAAAGACAGTATGATTTTTGTCAATGTTTGGGGAATTGGGAGAGACCCTTCAATTTGGGAAGATTCTCAAACGTTCAATCCTCAAAGATTTGATGTTGGTTGTGGTAATAATGTGGATTTTAAAGGGTATGATTATAGGTATTTGCCATTTGGTGGAGGAAGAAGAATTTGCCCTGGTCTGCCAATGGCCATTGTGCAAGTGCCTTTGATTTTAGCCACATTAATTCACAACTTTGAATGGTCACTTCCTAATGGTGAAGATTTATCAAAGTTGGATTTGAATGGAAGGCTTGGTGTAACATTGCAAAAGTATGAAACTCTTAAACTTATTCCTAAAAGGAGGGTTATTTGA

Coding sequence (CDS)

ATGACCCCTGATAGAGCTCTCCACGATCAGCTCTCTCTTGTTTTCTCCCCTCAATGTGGGGACCAATGGAAGAATTTGAAGTCAATTTGTAAAGGATCTATTGTGGAGATTAAAGATTTTGTTTTTACCTCTGTTTTCAATACACTTTCTAACTTGATTTTATCGATGGATTTGCTTGATTTTGTTGGTGATGGGTTTAACGGGATTAAAGGGCCTTTCAAGAAAATGATGAATTTGGGTTTGACTCCAAATTTGGCTGATTTTTATCCATTGTTGAGAAGGTTTGATCTTCAAGGTCTTAGAAAGAAAGCTGCCATTTACATGAATGAAGTTCATTCTTTGTGGGGGATTCTTATTAAAGAAAGAAGAGAAATTCATTGTAAAAAAGGTTCAGCTTCAAGTGATTTCTTGGATGTCTTGATTCAAAGTGGATTTAGTGATCAACAAATCAATGCCTTGATTGTTGAATTGCTATCTGCAGGCACAGACACTACTACAACAACAGTGGAATGGGCAATGTCAGAGATTCTAAAAGACAAAGACATACTAAACAAAGTAAGAGAGGAAATAAAGAAGGTAACAAAGGAAAACCCCATTGATGAATCCAACATTTCTAAGCTTCAATATCTATGGAGTTGTGTGAAAGAAACCCTAAGACTACACCCACCTGCCCCATTTCTTCTTCCACGTTTAGCTCCAACGGACTGTGAAGTTATGGGATACTCAATCCCAAAAGACAGTATGATTTTTGTCAATGTTTGGGGAATTGGGAGAGACCCTTCAATTTGGGAAGATTCTCAAACGTTCAATCCTCAAAGATTTGATGTTGGTTGTGGTAATAATGTGGATTTTAAAGGGTATGATTATAGGTATTTGCCATTTGGTGGAGGAAGAAGAATTTGCCCTGGTCTGCCAATGGCCATTGTGCAAGTGCCTTTGATTTTAGCCACATTAATTCACAACTTTGAATGGTCACTTCCTAATGGTGAAGATTTATCAAAGTTGGATTTGAATGGAAGGCTTGGTGTAACATTGCAAAAGTATGAAACTCTTAAACTTATTCCTAAAAGGAGGGTTATTTGA

Protein sequence

MTPDRALHDQLSLVFSPQCGDQWKNLKSICKGSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQINALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDLSKLDLNGRLGVTLQKYETLKLIPKRRVI*
Homology
BLAST of Chy9G166410 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.3e-82
Identity = 159/385 (41.30%), Postives = 235/385 (61.04%), Query Frame = 0

Query: 8   HDQLSLVFSPQCGDQWKNLKSICK-------------------------------GSIVE 67
           H + S+V+S +C D WK L+ +C+                               G +V+
Sbjct: 107 HVENSIVWS-ECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVK 166

Query: 68  IKDFVFTSVFNTLSNLILSMDLLDFVG--DGFNGIKGPFKKMMNLGLTPNLADFYPLLRR 127
           I + VF ++ N   NLI S D+ D      G   +K    K++++G + N AD++P++ +
Sbjct: 167 IVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGK 226

Query: 128 FDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSAS-SDFLDVLIQSGFSDQQINAL 187
            DL G R+  A  + +++ +WG+++KERR     KGS S +DF+DVL+ +G  DQ+INAL
Sbjct: 227 LDLFGQRRAVAEVLQQIYDVWGVMLKERRG---TKGSESKNDFVDVLLNAGLDDQKINAL 286

Query: 188 IVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQYLWSC 247
           ++EL  AGT+T+ +T+EWA++E+ K   +++K+R E+  V  +N + ES++  L YL + 
Sbjct: 287 LMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAF 346

Query: 248 VKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQR 307
           VKETLRLHPP P LLPR A   C VM Y+IPK+  I VN W IGRDP  W+D   F P+R
Sbjct: 347 VKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPER 406

Query: 308 FDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDLS 359
           F     ++VD+KG D+  +PFGGGRRICPGLP+A     LI+ATL+ NFEWSLP G   S
Sbjct: 407 F---LSSDVDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTS 466

BLAST of Chy9G166410 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.4e-81
Identity = 157/387 (40.57%), Postives = 232/387 (59.95%), Query Frame = 0

Query: 8   HDQLSLVFSPQCGDQWKNLKSICK-------------------------------GSIVE 67
           H + S+V+S  C + WKNL+ +C+                               G  V+
Sbjct: 106 HVENSIVWS-DCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVK 165

Query: 68  IKDFVFTSVFNTLSNLILSMDLLDFVGD---GFNGIKGPFKKMMNLGLTPNLADFYPLLR 127
           I + +F ++ N   NLI S ++ + +GD   G +  K    +M+ LG + N AD++P+L 
Sbjct: 166 IVEVIFGTLVNIFGNLIFSQNIFE-LGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLG 225

Query: 128 RFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKG-SASSDFLDVLIQSGFSDQQINA 187
           +FDL G RK+ A  +  ++++WG +++ER+      G  + +DF+DV + SG +D QINA
Sbjct: 226 KFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINA 285

Query: 188 LIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQYLWS 247
           L++EL  AGT+T+ +T+EWAM+E+ K+  I  K+R EI+ V  E  + ES+   L YL +
Sbjct: 286 LLMELFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEA 345

Query: 248 CVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQ 307
            VKETLRLHPP P LLPR A   C ++ Y+IPKD  I VN WGIGRDP  W D  TF+P+
Sbjct: 346 TVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPE 405

Query: 308 RFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDL 360
           RF     ++VDF+G D+  +PFG GRRICPGLP+A   + L++AT + N +W LPNG  +
Sbjct: 406 RF---LNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSV 465

BLAST of Chy9G166410 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.7e-80
Identity = 155/386 (40.16%), Postives = 229/386 (59.33%), Query Frame = 0

Query: 8   HDQLSLVFSPQCGDQWKNLKSICK-------------------------------GSIVE 67
           H + S+V+S  C + WKNL+ +C+                               G  V+
Sbjct: 107 HVENSIVWS-DCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVK 166

Query: 68  IKDFVFTSVFNTLSNLILSMDL--LDFVGDGFNGIKGPFKKMMNLGLTPNLADFYPLLRR 127
           I + +F ++ N   NLI S ++  L     G +  K    +M+ LG + N AD++P+L +
Sbjct: 167 IVEVIFGTLVNIFGNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGK 226

Query: 128 FDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKG-SASSDFLDVLIQSGFSDQQINAL 187
           FDL G RK+ A  +  ++++WG +++ER+      G  + +DF+DV + SG +D QINAL
Sbjct: 227 FDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINAL 286

Query: 188 IVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQYLWSC 247
           ++EL  AGT+T+ +T+EWAM+E+ K+  I  K+R E++ V  E  + ES+   L YL + 
Sbjct: 287 LMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEAT 346

Query: 248 VKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQR 307
           VKETLRLHPP P LLPR A   C ++ Y+IPKD  I VN WGIGRDP  W D  TF+P+R
Sbjct: 347 VKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPER 406

Query: 308 FDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDLS 360
           F     ++VDF+G D+  +PFG GRRICPGLP+A   + L++AT + N +W LPNG  + 
Sbjct: 407 F---LNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVD 466

BLAST of Chy9G166410 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 4.5e-80
Identity = 151/385 (39.22%), Postives = 230/385 (59.74%), Query Frame = 0

Query: 8   HDQLSLVFSPQCGDQWKNLKSICK-------------------------------GSIVE 67
           H + S+V+S +C + WK L+ +C+                               G+ V+
Sbjct: 101 HVENSIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVK 160

Query: 68  IKDFVFTSVFNTLSNLILSMDLLDFVGDGFNG---IKGPFKKMMNLGLTPNLADFYPLLR 127
           I + VF ++ N   NLI S ++   +GD  +G   +K    +M+ LG + N AD++P L 
Sbjct: 161 IAEVVFGTLVNIFGNLIFSQNIFK-LGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLG 220

Query: 128 RFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQINAL 187
           +FDL G  K  A  +  ++S+WG ++KER+       S  +DF+++L+ SG  DQQINAL
Sbjct: 221 KFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINAL 280

Query: 188 IVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQYLWSC 247
           ++E+  AGT+T+ +T+EWA+SE+ K+  +   +R E+  V  + P+ ES+I  + YL + 
Sbjct: 281 LMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAF 340

Query: 248 VKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQR 307
           VKETLRLHP  P LLPR A   C+V+ Y+IPK+  I VN WGIGRDP  W D   F+P+R
Sbjct: 341 VKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPER 400

Query: 308 FDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDLS 359
           F     +++DFKG D+  +PFG GRRICPG+P+A   + LI+++L+ NF+W  P G D S
Sbjct: 401 F---LNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPS 460

BLAST of Chy9G166410 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.0e-76
Identity = 151/384 (39.32%), Postives = 225/384 (58.59%), Query Frame = 0

Query: 8   HDQLSLVFSPQCGDQWKNLKSICK-------------------------------GSIVE 67
           H + SLV+S  C D WK L+ I +                               G +V+
Sbjct: 110 HIKYSLVWS-DCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVK 169

Query: 68  IKDFVFTSVFNTLSNLILSMDLLDFVGDGFN--GIKGPFKKMMNLGLTPNLADFYPLLRR 127
           I  FVF ++ N L N++ S D+  + GD  +  GI+   ++M+ +G  PN+A+FYP L  
Sbjct: 170 ISQFVFGTLLNILGNVVFSKDVFVY-GDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEE 229

Query: 128 FDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQINALI 187
            DLQGL+KK       V  +W   +KER+     +   S D LDVL+ + F+D QINAL 
Sbjct: 230 LDLQGLKKKCDERFIRVMKMWEGTVKERK---ANRNEESKDMLDVLLANDFNDAQINALF 289

Query: 188 VELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQYLWSCV 247
           +E    G++T++ T+EW ++E++K    + KVR+E+ +V   + I ES++ +L YL +C+
Sbjct: 290 LETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACI 349

Query: 248 KETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQRF 307
           KE +RLHP APFLLPR A   CEVMGY+IPK+S + VN + IGRDP  W+D  TF P+RF
Sbjct: 350 KEAMRLHPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERF 409

Query: 308 DVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDLSK 359
                ++VDF G  Y+++PFG GRR C G+P+A   +PLI+ +L+HN+++ LP G     
Sbjct: 410 ---LESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPED 469

BLAST of Chy9G166410 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.1e-190
Identity = 334/393 (84.99%), Postives = 349/393 (88.80%), Query Frame = 0

Query: 1   MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
           MTPDRALHDQ SLVFSP+CGDQWKNL+S+CK                             
Sbjct: 107 MTPDRALHDQHSLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLES 166

Query: 61  --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
             GS+VE+KDFVFTSVFNTLSNLI SMDLLDFVGDGF GIKGPFKKMM+LGLTPNLADFY
Sbjct: 167 KQGSVVEVKDFVFTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFY 226

Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
           PLLRRFDLQGL+ KA IY NEV SLWGILIKERREIHC++GSAS+DFLDVLIQSGF DQQ
Sbjct: 227 PLLRRFDLQGLKNKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQ 286

Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKEN-PIDESNISKLQ 240
           INALI+ELLSAGTDTTTTTVEWAMSEILKDKDILNKVREE+KKVTKEN PIDESNIS+LQ
Sbjct: 287 INALIIELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQ 346

Query: 241 YLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300
           YLW CVKETLRLHPPAPFLLPRLAP DCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQT
Sbjct: 347 YLWRCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQT 406

Query: 301 FNPQRFDVGCGN-NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 360
           FNPQRFDVGCGN NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP
Sbjct: 407 FNPQRFDVGCGNDNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 466

BLAST of Chy9G166410 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 1.9e-190
Identity = 333/393 (84.73%), Postives = 349/393 (88.80%), Query Frame = 0

Query: 1   MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
           MTPDRALHDQ SLVFSP+CGDQWKNL+S+CK                             
Sbjct: 107 MTPDRALHDQHSLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLES 166

Query: 61  --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
             GS+VE+KDFVFTSVFNTLSNLI SMDL+DFVGDGF GIKGPFKKMM+LGLTPNLADFY
Sbjct: 167 KQGSVVEVKDFVFTSVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFY 226

Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
           PLLRRFDLQGL+ KA IY NEV SLWGILIKERREIHC++GSAS+DFLDVLIQSGF DQQ
Sbjct: 227 PLLRRFDLQGLKNKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQ 286

Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKEN-PIDESNISKLQ 240
           INALI+ELLSAGTDTTTTTVEWAMSEILKDKDILNKVREE+KKVTKEN PIDESNIS+LQ
Sbjct: 287 INALIIELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQ 346

Query: 241 YLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300
           YLW CVKETLRLHPPAPFLLPRLAP DCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQT
Sbjct: 347 YLWRCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQT 406

Query: 301 FNPQRFDVGCGN-NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 360
           FNPQRFDVGCGN NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP
Sbjct: 407 FNPQRFDVGCGNDNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 466

BLAST of Chy9G166410 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.2e-152
Identity = 272/389 (69.92%), Postives = 307/389 (78.92%), Query Frame = 0

Query: 1   MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
           MTPDR LHD  SL+FSP+CGD+WKNL+SIC+                             
Sbjct: 104 MTPDRPLHDNCSLLFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQS 163

Query: 61  --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
             GS+VEIK+ VF +VFNTLSN I S DL+ FVGDGFNGIKGPFK M++LGL PNLADFY
Sbjct: 164 KQGSVVEIKELVFITVFNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFY 223

Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
           PLLR FDLQGLRKKAAIY N+V S W ILIKERR IH +  SA+SDFLDVLIQSGF+D+Q
Sbjct: 224 PLLRCFDLQGLRKKAAIYKNQVDSSWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQ 283

Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQY 240
           I+ LIVE+LSAGTDTTTTTVEWAM+E+LK++DI+N+VREE KK      IDES +S+L Y
Sbjct: 284 ISYLIVEVLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHY 343

Query: 241 LWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF 300
           LW CVKETLRLHPPAPFLLPR AP  CE+MGYS+PK +MIFVNVWGIGRDPSIWED Q+F
Sbjct: 344 LWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSF 403

Query: 301 NPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNG 359
            P+RFD GC  + D KG+DYR+LPFGGGRRICPGLPMA VQVPLILATLIHNFEW LPN 
Sbjct: 404 KPERFD-GC--DADLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPND 463

BLAST of Chy9G166410 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 3.0e-151
Identity = 268/389 (68.89%), Postives = 306/389 (78.66%), Query Frame = 0

Query: 1   MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
           MTPDR LHD  SL+FSP+CGD+WKNL+SIC+                             
Sbjct: 104 MTPDRPLHDNCSLLFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQS 163

Query: 61  --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
             GS+VEIK+ VF +VFNTLSN I S DL+ FVGDGFNGIKG FK +++LGL PNLADFY
Sbjct: 164 KQGSVVEIKELVFITVFNTLSNFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFY 223

Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
           PLLR FDLQGLRKKAAIY N+V S W ILIKERR IH +  SA+SDFLDVLI+SGF+D+Q
Sbjct: 224 PLLRSFDLQGLRKKAAIYKNQVDSSWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQ 283

Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQY 240
           I+ LIVE+LSAGTDTTTTTVEWAM+E+LK++DI+N+VREE KK      IDES +S+L Y
Sbjct: 284 ISYLIVEVLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHY 343

Query: 241 LWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF 300
           LW CVKETLRLHPPAPFLLPR AP  CE+MGYS+PKD+MIFVNVWGIGRDPSIWED  +F
Sbjct: 344 LWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSF 403

Query: 301 NPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNG 359
            P+RFD   G + D KG+DYR+LPFGGGRRICPGLPMA VQVPLILATLIHNFEW LPN 
Sbjct: 404 KPERFD---GFDADLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPND 463

BLAST of Chy9G166410 vs. ExPASy TrEMBL
Match: A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 9.6e-150
Identity = 266/390 (68.21%), Postives = 305/390 (78.21%), Query Frame = 0

Query: 1   MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
           M PDRALHD  SLVFSP+CGD+WKNL+SIC+                             
Sbjct: 103 MIPDRALHDNCSLVFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQS 162

Query: 61  --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
             GS+VEIK+FVF +VFNTLSN I S DL+ FVGDGFNGI+G FK +++  L PNLADFY
Sbjct: 163 KQGSVVEIKEFVFITVFNTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFY 222

Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
           PLLR FDLQGLRKKAAIY N++ S W ILIKERR IH +    +SDFLDVLIQSGF+D+Q
Sbjct: 223 PLLRCFDLQGLRKKAAIYKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQ 282

Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQY 240
           IN LIVE+LSAGTDTTTTTVEWAM+E+LK++DI+N+VREE KK    + IDES +S+L Y
Sbjct: 283 INYLIVEVLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHY 342

Query: 241 LWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF 300
           LW CVKETLRLHPPAPFLLPR AP  CE+MGYS+PK +MI VNVWGIGRDPSIWED Q+F
Sbjct: 343 LWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSF 402

Query: 301 NPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNG 360
            P+RFDV C  + D KG+DYR+LPFGGGRR+CPGLPMA VQVPLILATLIHNFEW LPN 
Sbjct: 403 KPERFDV-C--DADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPND 462

BLAST of Chy9G166410 vs. NCBI nr
Match: XP_031741123.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 675 bits (1741), Expect = 1.09e-241
Identity = 334/391 (85.42%), Postives = 348/391 (89.00%), Query Frame = 0

Query: 1   MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
           MTPDRAL++Q SLVFSP+CGD WKNL+SICK                             
Sbjct: 109 MTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLES 168

Query: 61  --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
             GS+V+IKDFVFTSVFNTLSNLI SMDLLDFVGDGFNGIKG FKKMM+LGLTPNLADFY
Sbjct: 169 KQGSVVKIKDFVFTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFY 228

Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
           PLLRRFDLQGLRKKAAIYMNEV SLWGILIKERREIHC++GSASSDFLDVLIQSGFSDQQ
Sbjct: 229 PLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQ 288

Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQY 240
           IN LI ELLSAGTDTTTTTVEWAMSEILKDK ILNKVR+EIKKVTKE+PIDESNISKLQY
Sbjct: 289 INYLINELLSAGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQY 348

Query: 241 LWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF 300
           LWSCVKETLRLHPPAPFLLPRLAP DCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF
Sbjct: 349 LWSCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF 408

Query: 301 NPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNG 360
           NP+RFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPNG
Sbjct: 409 NPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNG 468

BLAST of Chy9G166410 vs. NCBI nr
Match: XP_008467223.1 (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])

HSP 1 Score: 674 bits (1740), Expect = 1.54e-241
Identity = 334/393 (84.99%), Postives = 349/393 (88.80%), Query Frame = 0

Query: 1   MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
           MTPDRALHDQ SLVFSP+CGDQWKNL+S+CK                             
Sbjct: 107 MTPDRALHDQHSLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLES 166

Query: 61  --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
             GS+VE+KDFVFTSVFNTLSNLI SMDLLDFVGDGF GIKGPFKKMM+LGLTPNLADFY
Sbjct: 167 KQGSVVEVKDFVFTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFY 226

Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
           PLLRRFDLQGL+ KA IY NEV SLWGILIKERREIHC++GSAS+DFLDVLIQSGF DQQ
Sbjct: 227 PLLRRFDLQGLKNKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQ 286

Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKEN-PIDESNISKLQ 240
           INALI+ELLSAGTDTTTTTVEWAMSEILKDKDILNKVREE+KKVTKEN PIDESNIS+LQ
Sbjct: 287 INALIIELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQ 346

Query: 241 YLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300
           YLW CVKETLRLHPPAPFLLPRLAP DCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQT
Sbjct: 347 YLWRCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQT 406

Query: 301 FNPQRFDVGCGN-NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 360
           FNPQRFDVGCGN NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP
Sbjct: 407 FNPQRFDVGCGNDNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 466

BLAST of Chy9G166410 vs. NCBI nr
Match: KAA0055170.1 (putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. makuwa])

HSP 1 Score: 674 bits (1738), Expect = 3.11e-241
Identity = 333/393 (84.73%), Postives = 349/393 (88.80%), Query Frame = 0

Query: 1   MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
           MTPDRALHDQ SLVFSP+CGDQWKNL+S+CK                             
Sbjct: 107 MTPDRALHDQHSLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLES 166

Query: 61  --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
             GS+VE+KDFVFTSVFNTLSNLI SMDL+DFVGDGF GIKGPFKKMM+LGLTPNLADFY
Sbjct: 167 KQGSVVEVKDFVFTSVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFY 226

Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
           PLLRRFDLQGL+ KA IY NEV SLWGILIKERREIHC++GSAS+DFLDVLIQSGF DQQ
Sbjct: 227 PLLRRFDLQGLKNKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQ 286

Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKEN-PIDESNISKLQ 240
           INALI+ELLSAGTDTTTTTVEWAMSEILKDKDILNKVREE+KKVTKEN PIDESNIS+LQ
Sbjct: 287 INALIIELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQ 346

Query: 241 YLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300
           YLW CVKETLRLHPPAPFLLPRLAP DCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQT
Sbjct: 347 YLWRCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQT 406

Query: 301 FNPQRFDVGCGN-NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 360
           FNPQRFDVGCGN NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP
Sbjct: 407 FNPQRFDVGCGNDNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 466

BLAST of Chy9G166410 vs. NCBI nr
Match: KAE8647950.1 (hypothetical protein Csa_000545 [Cucumis sativus])

HSP 1 Score: 671 bits (1730), Expect = 5.32e-240
Identity = 333/392 (84.95%), Postives = 347/392 (88.52%), Query Frame = 0

Query: 1   MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
           MTPDRAL++Q SLVFSP+CGD WKNL+SICK                             
Sbjct: 109 MTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLES 168

Query: 61  --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
             GS+V+IKDFVFTSVFNTLSNLI SMDLLDFVGDGFNGIKG FKKMM+LGLTPNLADFY
Sbjct: 169 KQGSVVKIKDFVFTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFY 228

Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
           PLLRRFDLQGLRKKAAIYMNEV SLWGILIKERREIHC++GSASSDFLDVLIQSGFSDQQ
Sbjct: 229 PLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQ 288

Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENP-IDESNISKLQ 240
           IN LI ELL A TDTTTTTVEWAMSEILKDKDILNK REEIKKV KE+P IDESNISKLQ
Sbjct: 289 INYLINELLFAATDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQ 348

Query: 241 YLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300
           YLWSCVKETLRLHPP PFLLPRLAP DCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT
Sbjct: 349 YLWSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 408

Query: 301 FNPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPN 360
           FNP+RFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN
Sbjct: 409 FNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPN 468

BLAST of Chy9G166410 vs. NCBI nr
Match: XP_031741076.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 658 bits (1697), Expect = 8.04e-235
Identity = 325/392 (82.91%), Postives = 342/392 (87.24%), Query Frame = 0

Query: 1   MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
           +TPDRALHDQ SLVFS +CGD WK L+SICK                             
Sbjct: 119 ITPDRALHDQHSLVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVES 178

Query: 61  --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
             GS+VEIKDFVFTS FN LSNLI SMDLLDFVGDGFNGIKGPFKKMM+LGLTPNLADFY
Sbjct: 179 KQGSVVEIKDFVFTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFY 238

Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
           PLLRRFDLQGL +K  IY NEV+S+WGILIKERREI C+KGSAS+DFLDVLIQSGFSDQQ
Sbjct: 239 PLLRRFDLQGLIRKTVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQ 298

Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENP-IDESNISKLQ 240
           I+ALI+ELL A TDTTTTTVEWAMSEILKDKDILNK REEIKKV KE+P IDESNISKLQ
Sbjct: 299 IDALIIELLFAATDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQ 358

Query: 241 YLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300
           YLWSCVKETLRLHPP PFLLPRLAP DCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT
Sbjct: 359 YLWSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 418

Query: 301 FNPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPN 360
           FNP+RFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN
Sbjct: 419 FNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPN 478

BLAST of Chy9G166410 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 259.6 bits (662), Expect = 3.7e-69
Identity = 133/339 (39.23%), Postives = 208/339 (61.36%), Query Frame = 0

Query: 35  VEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFK--KMMNLGLTPNLADFYPLL 94
           +++  + F   FN + NL+ S DLLD   D   G +  +   K+M     PN+ADF+PLL
Sbjct: 175 IDVGRYFFLMAFNLIGNLMFSRDLLD--PDSKRGSEFFYHTGKVMEFAGKPNVADFFPLL 234

Query: 95  RRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGS--ASSDFLDVLIQ-------- 154
           R  D QG+R+K   ++ +   + G  I+ER E+  ++ S   + D+LDVL++        
Sbjct: 235 RFLDPQGIRRKTQFHVEKAFEIAGEFIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVD 294

Query: 155 ---SGFSDQQINALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKEN-- 214
              S FS + IN ++ E+ +AGTDTTT+T+EWA++E+L +   L K++ E++   K +  
Sbjct: 295 EEPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQ 354

Query: 215 PIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPTDCEVMG-YSIPKDSMIFVNVWGI 274
            + E ++  L YL + + ETLRLHPP PFL+P  A + C +   Y+IPK++ + VNVW I
Sbjct: 355 KLQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAI 414

Query: 275 GRDPSIWEDSQTFNPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILA 334
           GRDP  W D   F P+RF +   +  DFKG DY +LPFG GRR+CP LP+A   +PL + 
Sbjct: 415 GRDPKTWIDPIMFKPERF-ISDPDARDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIG 474

Query: 335 TLIHNFEWSLPNGEDLSKLDLNGRLGVTLQKYETLKLIP 356
           +++ +F+W+L NG +  ++D+  R+G+TL+K   L+ IP
Sbjct: 475 SMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAIP 510

BLAST of Chy9G166410 vs. TAIR 10
Match: AT3G52970.2 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 259.6 bits (662), Expect = 3.7e-69
Identity = 133/339 (39.23%), Postives = 208/339 (61.36%), Query Frame = 0

Query: 35  VEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFK--KMMNLGLTPNLADFYPLL 94
           +++  + F   FN + NL+ S DLLD   D   G +  +   K+M     PN+ADF+PLL
Sbjct: 189 IDVGRYFFLMAFNLIGNLMFSRDLLD--PDSKRGSEFFYHTGKVMEFAGKPNVADFFPLL 248

Query: 95  RRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGS--ASSDFLDVLIQ-------- 154
           R  D QG+R+K   ++ +   + G  I+ER E+  ++ S   + D+LDVL++        
Sbjct: 249 RFLDPQGIRRKTQFHVEKAFEIAGEFIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVD 308

Query: 155 ---SGFSDQQINALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKEN-- 214
              S FS + IN ++ E+ +AGTDTTT+T+EWA++E+L +   L K++ E++   K +  
Sbjct: 309 EEPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQ 368

Query: 215 PIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPTDCEVMG-YSIPKDSMIFVNVWGI 274
            + E ++  L YL + + ETLRLHPP PFL+P  A + C +   Y+IPK++ + VNVW I
Sbjct: 369 KLQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAI 428

Query: 275 GRDPSIWEDSQTFNPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILA 334
           GRDP  W D   F P+RF +   +  DFKG DY +LPFG GRR+CP LP+A   +PL + 
Sbjct: 429 GRDPKTWIDPIMFKPERF-ISDPDARDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIG 488

Query: 335 TLIHNFEWSLPNGEDLSKLDLNGRLGVTLQKYETLKLIP 356
           +++ +F+W+L NG +  ++D+  R+G+TL+K   L+ IP
Sbjct: 489 SMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAIP 524

BLAST of Chy9G166410 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 256.5 bits (654), Expect = 3.1e-68
Identity = 150/377 (39.79%), Postives = 217/377 (57.56%), Query Frame = 0

Query: 14  VFSPQCGDQWKNLK-------------SICKGSIVEIKDFVFTSVFNTLSNLILSMDLLD 73
           +FSPQC    K L+             S  +   V+I    F +  N +SN++ S++L  
Sbjct: 142 LFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISHVSFVTALNIISNILFSVNLGS 201

Query: 74  FVGDGFNGIKGPFKKMM-----NLGLTPNLADFYPLLRRFDLQG----LRKKAAIYMNEV 133
           +  D  N     F++M+     ++G  P+LA+F+P +R  DLQG    +R+ +   +   
Sbjct: 202 Y--DSKN--SSAFQEMVIGYQESIG-NPDLANFFPFMRFLDLQGNSKKMRESSGRLLQVF 261

Query: 134 HSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQ------QINALIVELLSAGTDTT 193
              +   I E+     +K  +S DFLDVLI     D+      +I  L++++  AGTDT 
Sbjct: 262 REFYDARIVEKSSRSVEKDVSSKDFLDVLIDLQQGDETEINIDEIEHLLLDMFVAGTDTN 321

Query: 194 TTTVEWAMSEILKDKDILNKVREEIKKVTKEN-PIDESNISKLQYLWSCVKETLRLHPPA 253
           ++TVEWAM+E+L +   + KV++EI  V  +N    ES+ISKL YL + VKET RLHP A
Sbjct: 322 SSTVEWAMAELLGNPKTMTKVQDEINHVIGQNGDFQESDISKLPYLKAVVKETFRLHPAA 381

Query: 254 PFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQRFDVGCGNNVDF 313
           PFLL R A T+ E++G+++ KDS + VNVW IGRDP +WE+   F P+RF    G  +D 
Sbjct: 382 PFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWENPTHFEPERF---LGKEIDV 441

Query: 314 KGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDLSKLDLNGRLGVT 361
           KG DY   PFG GRRICPGLP+A+  V L+LA+L++ FEW LPNG     LD+    G+T
Sbjct: 442 KGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLPNGVGSEDLDMEETFGLT 501

BLAST of Chy9G166410 vs. TAIR 10
Match: AT1G33730.1 (cytochrome P450, family 76, subfamily C, polypeptide 5 )

HSP 1 Score: 255.8 bits (652), Expect = 5.3e-68
Identity = 133/332 (40.06%), Postives = 199/332 (59.94%), Query Frame = 0

Query: 31  KGSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFYP 90
           +G  V+I    F +  N +SN++ S++L  +     +  +      M     P++++F+P
Sbjct: 36  RGEAVDISRASFVTALNIISNILFSVNLGSYDSKNSSAFQEMVIGYMESIGNPDVSNFFP 95

Query: 91  LLRRFDLQGLRKKAAIYMNEV----HSLWGILIKERREIHCKKGSASSDFLDVLI----- 150
            +R  DLQG  KK   Y  ++       +   I E      +K  +S DFLD LI     
Sbjct: 96  FMRLLDLQGNSKKMKEYSGKLLQVFREFYDARILENSSRIDEKDVSSRDFLDALIDLQQG 155

Query: 151 -QSGFSDQQINALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKEN-PI 210
            +S  +  +I  L++++  AGTDT ++TVEWAM+E+L +   + KV++EI +V ++N  +
Sbjct: 156 DESEINIDEIEHLLLDMFLAGTDTNSSTVEWAMTELLGNPKTMTKVQDEINRVIRQNGDV 215

Query: 211 DESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRD 270
            ES+ISKL YL + +KET RLHP APFLLPR A  D +++G+ +PKDS + VNVW IGRD
Sbjct: 216 QESHISKLPYLQAVIKETFRLHPAAPFLLPRKAERDVDILGFHVPKDSHVLVNVWAIGRD 275

Query: 271 PSIWEDSQTFNPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLI 330
           P++WE+   F P+RF    G ++D KG +Y   PFG GRRICPGLP+A+  V L+LA+L+
Sbjct: 276 PNVWENPTQFEPERF---LGKDIDVKGTNYELTPFGAGRRICPGLPLALKTVHLMLASLL 335

Query: 331 HNFEWSLPNGEDLSKLDLNGRLGVTLQKYETL 352
           + FEW LPNG     LD+    G+T+ K   L
Sbjct: 336 YTFEWKLPNGVGSEDLDMGETFGLTVHKTNPL 364

BLAST of Chy9G166410 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 255.8 bits (652), Expect = 5.3e-68
Identity = 140/372 (37.63%), Postives = 219/372 (58.87%), Query Frame = 0

Query: 11  LSLVFSPQCGDQWKNLK-------------SICKGSIVEIKDFVFTSVFNTLSNLILSMD 70
           ++L+FSPQ  +  K L+             S  +   V+I    +T+V N +SN++ S+D
Sbjct: 139 VTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNIISNILFSVD 198

Query: 71  LLDFVGDGFNGIKGPFKKMMNLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVHSLW-G 130
           L  +     N  +      M     P+ A+++P +   DLQG RK        +  ++ G
Sbjct: 199 LGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTERLFRVFRG 258

Query: 131 IL---IKERREIHCKKGSASSDFLDVLI------QSGFSDQQINALIVELLSAGTDTTTT 190
            +   I E+   +  K  ++ DFLD L+      ++   +  I  L++++ +AGTDT+++
Sbjct: 259 FMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLLLDMFTAGTDTSSS 318

Query: 191 TVEWAMSEILKDKDILNKVREEIKKVTKENP-IDESNISKLQYLWSCVKETLRLHPPAPF 250
           T+EWAM+E+L++   + K + E+ +V  +N  + ES+IS L YL + VKET RLHP AP 
Sbjct: 319 TLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETFRLHPAAPL 378

Query: 251 LLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQRFDVGCGNNVDFKG 310
           L+PR A +D EV+G+ +PKD+ + VNVW IGRDPS+WE+   F P+RF    G ++D KG
Sbjct: 379 LVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERF---MGKDIDVKG 438

Query: 311 YDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDLSKLDLNGRLGVTLQ 359
            DY   PFGGGRRICPGLP+A+  V L+LA+L+++F+W LPNG     LD++   G+TL 
Sbjct: 439 RDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITLH 498

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FXW41.3e-8241.30Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3... [more]
O648992.4e-8140.57(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif... [more]
O649002.7e-8040.16(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3... [more]
Q9SP064.5e-8039.22(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum... [more]
A8CDR53.0e-7639.32Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CT141.1e-19084.99probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656... [more]
A0A5A7UIU31.9e-19084.73Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma... [more]
A0A6J1ELA61.2e-15269.92probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... [more]
A0A6J1I4V73.0e-15168.89probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=... [more]
A0A6J1ER529.6e-15068.21probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... [more]
Match NameE-valueIdentityDescription
XP_031741123.11.09e-24185.42probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus][more]
XP_008467223.11.54e-24184.99PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis mel... [more]
KAA0055170.13.11e-24184.73putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. maku... [more]
KAE8647950.15.32e-24084.95hypothetical protein Csa_000545 [Cucumis sativus][more]
XP_031741076.18.04e-23582.91probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT3G52970.13.7e-6939.23cytochrome P450, family 76, subfamily G, polypeptide 1 [more]
AT3G52970.23.7e-6939.23cytochrome P450, family 76, subfamily G, polypeptide 1 [more]
AT1G33720.13.1e-6839.79cytochrome P450, family 76, subfamily C, polypeptide 6 [more]
AT1G33730.15.3e-6840.06cytochrome P450, family 76, subfamily C, polypeptide 5 [more]
AT2G45550.15.3e-6837.63cytochrome P450, family 76, subfamily C, polypeptide 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 301..312
score: 28.57
coord: 160..177
score: 36.59
coord: 212..223
score: 40.07
coord: 292..301
score: 60.95
IPR001128Cytochrome P450PFAMPF00067p450coord: 33..342
e-value: 8.3E-74
score: 249.0
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 211..229
score: 32.67
coord: 149..166
score: 31.98
coord: 301..324
score: 31.51
coord: 252..276
score: 31.9
coord: 291..301
score: 55.82
coord: 169..195
score: 26.2
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 2..359
e-value: 3.1E-82
score: 278.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 22..358
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 31..356
NoneNo IPR availablePANTHERPTHR47956:SF23(S)-N-METHYLCOCLAURINE 3'-HYDROXYLASE ISOZYME 1-LIKEcoord: 31..356
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 294..303

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy9G166410.1Chy9G166410.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen