Homology
BLAST of Chy9G166410 vs. ExPASy Swiss-Prot
Match:
Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)
HSP 1 Score: 308.1 bits (788), Expect = 1.3e-82
Identity = 159/385 (41.30%), Postives = 235/385 (61.04%), Query Frame = 0
Query: 8 HDQLSLVFSPQCGDQWKNLKSICK-------------------------------GSIVE 67
H + S+V+S +C D WK L+ +C+ G +V+
Sbjct: 107 HVENSIVWS-ECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVK 166
Query: 68 IKDFVFTSVFNTLSNLILSMDLLDFVG--DGFNGIKGPFKKMMNLGLTPNLADFYPLLRR 127
I + VF ++ N NLI S D+ D G +K K++++G + N AD++P++ +
Sbjct: 167 IVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGK 226
Query: 128 FDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSAS-SDFLDVLIQSGFSDQQINAL 187
DL G R+ A + +++ +WG+++KERR KGS S +DF+DVL+ +G DQ+INAL
Sbjct: 227 LDLFGQRRAVAEVLQQIYDVWGVMLKERRG---TKGSESKNDFVDVLLNAGLDDQKINAL 286
Query: 188 IVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQYLWSC 247
++EL AGT+T+ +T+EWA++E+ K +++K+R E+ V +N + ES++ L YL +
Sbjct: 287 LMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAF 346
Query: 248 VKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQR 307
VKETLRLHPP P LLPR A C VM Y+IPK+ I VN W IGRDP W+D F P+R
Sbjct: 347 VKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPER 406
Query: 308 FDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDLS 359
F ++VD+KG D+ +PFGGGRRICPGLP+A LI+ATL+ NFEWSLP G S
Sbjct: 407 F---LSSDVDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTS 466
BLAST of Chy9G166410 vs. ExPASy Swiss-Prot
Match:
O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)
HSP 1 Score: 303.9 bits (777), Expect = 2.4e-81
Identity = 157/387 (40.57%), Postives = 232/387 (59.95%), Query Frame = 0
Query: 8 HDQLSLVFSPQCGDQWKNLKSICK-------------------------------GSIVE 67
H + S+V+S C + WKNL+ +C+ G V+
Sbjct: 106 HVENSIVWS-DCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVK 165
Query: 68 IKDFVFTSVFNTLSNLILSMDLLDFVGD---GFNGIKGPFKKMMNLGLTPNLADFYPLLR 127
I + +F ++ N NLI S ++ + +GD G + K +M+ LG + N AD++P+L
Sbjct: 166 IVEVIFGTLVNIFGNLIFSQNIFE-LGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLG 225
Query: 128 RFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKG-SASSDFLDVLIQSGFSDQQINA 187
+FDL G RK+ A + ++++WG +++ER+ G + +DF+DV + SG +D QINA
Sbjct: 226 KFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINA 285
Query: 188 LIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQYLWS 247
L++EL AGT+T+ +T+EWAM+E+ K+ I K+R EI+ V E + ES+ L YL +
Sbjct: 286 LLMELFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEA 345
Query: 248 CVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQ 307
VKETLRLHPP P LLPR A C ++ Y+IPKD I VN WGIGRDP W D TF+P+
Sbjct: 346 TVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPE 405
Query: 308 RFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDL 360
RF ++VDF+G D+ +PFG GRRICPGLP+A + L++AT + N +W LPNG +
Sbjct: 406 RF---LNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSV 465
BLAST of Chy9G166410 vs. ExPASy Swiss-Prot
Match:
O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)
HSP 1 Score: 300.4 bits (768), Expect = 2.7e-80
Identity = 155/386 (40.16%), Postives = 229/386 (59.33%), Query Frame = 0
Query: 8 HDQLSLVFSPQCGDQWKNLKSICK-------------------------------GSIVE 67
H + S+V+S C + WKNL+ +C+ G V+
Sbjct: 107 HVENSIVWS-DCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVK 166
Query: 68 IKDFVFTSVFNTLSNLILSMDL--LDFVGDGFNGIKGPFKKMMNLGLTPNLADFYPLLRR 127
I + +F ++ N NLI S ++ L G + K +M+ LG + N AD++P+L +
Sbjct: 167 IVEVIFGTLVNIFGNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGK 226
Query: 128 FDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKG-SASSDFLDVLIQSGFSDQQINAL 187
FDL G RK+ A + ++++WG +++ER+ G + +DF+DV + SG +D QINAL
Sbjct: 227 FDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINAL 286
Query: 188 IVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQYLWSC 247
++EL AGT+T+ +T+EWAM+E+ K+ I K+R E++ V E + ES+ L YL +
Sbjct: 287 LMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEAT 346
Query: 248 VKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQR 307
VKETLRLHPP P LLPR A C ++ Y+IPKD I VN WGIGRDP W D TF+P+R
Sbjct: 347 VKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPER 406
Query: 308 FDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDLS 360
F ++VDF+G D+ +PFG GRRICPGLP+A + L++AT + N +W LPNG +
Sbjct: 407 F---LNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVD 466
BLAST of Chy9G166410 vs. ExPASy Swiss-Prot
Match:
Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)
HSP 1 Score: 299.7 bits (766), Expect = 4.5e-80
Identity = 151/385 (39.22%), Postives = 230/385 (59.74%), Query Frame = 0
Query: 8 HDQLSLVFSPQCGDQWKNLKSICK-------------------------------GSIVE 67
H + S+V+S +C + WK L+ +C+ G+ V+
Sbjct: 101 HVENSIVWS-ECNETWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVK 160
Query: 68 IKDFVFTSVFNTLSNLILSMDLLDFVGDGFNG---IKGPFKKMMNLGLTPNLADFYPLLR 127
I + VF ++ N NLI S ++ +GD +G +K +M+ LG + N AD++P L
Sbjct: 161 IAEVVFGTLVNIFGNLIFSQNIFK-LGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLG 220
Query: 128 RFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQINAL 187
+FDL G K A + ++S+WG ++KER+ S +DF+++L+ SG DQQINAL
Sbjct: 221 KFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINAL 280
Query: 188 IVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQYLWSC 247
++E+ AGT+T+ +T+EWA+SE+ K+ + +R E+ V + P+ ES+I + YL +
Sbjct: 281 LMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAF 340
Query: 248 VKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQR 307
VKETLRLHP P LLPR A C+V+ Y+IPK+ I VN WGIGRDP W D F+P+R
Sbjct: 341 VKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPER 400
Query: 308 FDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDLS 359
F +++DFKG D+ +PFG GRRICPG+P+A + LI+++L+ NF+W P G D S
Sbjct: 401 F---LNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPS 460
BLAST of Chy9G166410 vs. ExPASy Swiss-Prot
Match:
A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)
HSP 1 Score: 287.0 bits (733), Expect = 3.0e-76
Identity = 151/384 (39.32%), Postives = 225/384 (58.59%), Query Frame = 0
Query: 8 HDQLSLVFSPQCGDQWKNLKSICK-------------------------------GSIVE 67
H + SLV+S C D WK L+ I + G +V+
Sbjct: 110 HIKYSLVWS-DCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVK 169
Query: 68 IKDFVFTSVFNTLSNLILSMDLLDFVGDGFN--GIKGPFKKMMNLGLTPNLADFYPLLRR 127
I FVF ++ N L N++ S D+ + GD + GI+ ++M+ +G PN+A+FYP L
Sbjct: 170 ISQFVFGTLLNILGNVVFSKDVFVY-GDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEE 229
Query: 128 FDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQINALI 187
DLQGL+KK V +W +KER+ + S D LDVL+ + F+D QINAL
Sbjct: 230 LDLQGLKKKCDERFIRVMKMWEGTVKERK---ANRNEESKDMLDVLLANDFNDAQINALF 289
Query: 188 VELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQYLWSCV 247
+E G++T++ T+EW ++E++K + KVR+E+ +V + I ES++ +L YL +C+
Sbjct: 290 LETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACI 349
Query: 248 KETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQRF 307
KE +RLHP APFLLPR A CEVMGY+IPK+S + VN + IGRDP W+D TF P+RF
Sbjct: 350 KEAMRLHPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERF 409
Query: 308 DVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDLSK 359
++VDF G Y+++PFG GRR C G+P+A +PLI+ +L+HN+++ LP G
Sbjct: 410 ---LESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPED 469
BLAST of Chy9G166410 vs. ExPASy TrEMBL
Match:
A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)
HSP 1 Score: 675.6 bits (1742), Expect = 1.1e-190
Identity = 334/393 (84.99%), Postives = 349/393 (88.80%), Query Frame = 0
Query: 1 MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
MTPDRALHDQ SLVFSP+CGDQWKNL+S+CK
Sbjct: 107 MTPDRALHDQHSLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLES 166
Query: 61 --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
GS+VE+KDFVFTSVFNTLSNLI SMDLLDFVGDGF GIKGPFKKMM+LGLTPNLADFY
Sbjct: 167 KQGSVVEVKDFVFTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFY 226
Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
PLLRRFDLQGL+ KA IY NEV SLWGILIKERREIHC++GSAS+DFLDVLIQSGF DQQ
Sbjct: 227 PLLRRFDLQGLKNKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQ 286
Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKEN-PIDESNISKLQ 240
INALI+ELLSAGTDTTTTTVEWAMSEILKDKDILNKVREE+KKVTKEN PIDESNIS+LQ
Sbjct: 287 INALIIELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQ 346
Query: 241 YLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300
YLW CVKETLRLHPPAPFLLPRLAP DCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQT
Sbjct: 347 YLWRCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQT 406
Query: 301 FNPQRFDVGCGN-NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 360
FNPQRFDVGCGN NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP
Sbjct: 407 FNPQRFDVGCGNDNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 466
BLAST of Chy9G166410 vs. ExPASy TrEMBL
Match:
A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)
HSP 1 Score: 674.9 bits (1740), Expect = 1.9e-190
Identity = 333/393 (84.73%), Postives = 349/393 (88.80%), Query Frame = 0
Query: 1 MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
MTPDRALHDQ SLVFSP+CGDQWKNL+S+CK
Sbjct: 107 MTPDRALHDQHSLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLES 166
Query: 61 --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
GS+VE+KDFVFTSVFNTLSNLI SMDL+DFVGDGF GIKGPFKKMM+LGLTPNLADFY
Sbjct: 167 KQGSVVEVKDFVFTSVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFY 226
Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
PLLRRFDLQGL+ KA IY NEV SLWGILIKERREIHC++GSAS+DFLDVLIQSGF DQQ
Sbjct: 227 PLLRRFDLQGLKNKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQ 286
Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKEN-PIDESNISKLQ 240
INALI+ELLSAGTDTTTTTVEWAMSEILKDKDILNKVREE+KKVTKEN PIDESNIS+LQ
Sbjct: 287 INALIIELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQ 346
Query: 241 YLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300
YLW CVKETLRLHPPAPFLLPRLAP DCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQT
Sbjct: 347 YLWRCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQT 406
Query: 301 FNPQRFDVGCGN-NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 360
FNPQRFDVGCGN NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP
Sbjct: 407 FNPQRFDVGCGNDNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 466
BLAST of Chy9G166410 vs. ExPASy TrEMBL
Match:
A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)
HSP 1 Score: 549.3 bits (1414), Expect = 1.2e-152
Identity = 272/389 (69.92%), Postives = 307/389 (78.92%), Query Frame = 0
Query: 1 MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
MTPDR LHD SL+FSP+CGD+WKNL+SIC+
Sbjct: 104 MTPDRPLHDNCSLLFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQS 163
Query: 61 --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
GS+VEIK+ VF +VFNTLSN I S DL+ FVGDGFNGIKGPFK M++LGL PNLADFY
Sbjct: 164 KQGSVVEIKELVFITVFNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFY 223
Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
PLLR FDLQGLRKKAAIY N+V S W ILIKERR IH + SA+SDFLDVLIQSGF+D+Q
Sbjct: 224 PLLRCFDLQGLRKKAAIYKNQVDSSWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQ 283
Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQY 240
I+ LIVE+LSAGTDTTTTTVEWAM+E+LK++DI+N+VREE KK IDES +S+L Y
Sbjct: 284 ISYLIVEVLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHY 343
Query: 241 LWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF 300
LW CVKETLRLHPPAPFLLPR AP CE+MGYS+PK +MIFVNVWGIGRDPSIWED Q+F
Sbjct: 344 LWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSF 403
Query: 301 NPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNG 359
P+RFD GC + D KG+DYR+LPFGGGRRICPGLPMA VQVPLILATLIHNFEW LPN
Sbjct: 404 KPERFD-GC--DADLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPND 463
BLAST of Chy9G166410 vs. ExPASy TrEMBL
Match:
A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)
HSP 1 Score: 544.7 bits (1402), Expect = 3.0e-151
Identity = 268/389 (68.89%), Postives = 306/389 (78.66%), Query Frame = 0
Query: 1 MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
MTPDR LHD SL+FSP+CGD+WKNL+SIC+
Sbjct: 104 MTPDRPLHDNCSLLFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQS 163
Query: 61 --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
GS+VEIK+ VF +VFNTLSN I S DL+ FVGDGFNGIKG FK +++LGL PNLADFY
Sbjct: 164 KQGSVVEIKELVFITVFNTLSNFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFY 223
Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
PLLR FDLQGLRKKAAIY N+V S W ILIKERR IH + SA+SDFLDVLI+SGF+D+Q
Sbjct: 224 PLLRSFDLQGLRKKAAIYKNQVDSSWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQ 283
Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQY 240
I+ LIVE+LSAGTDTTTTTVEWAM+E+LK++DI+N+VREE KK IDES +S+L Y
Sbjct: 284 ISYLIVEVLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHY 343
Query: 241 LWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF 300
LW CVKETLRLHPPAPFLLPR AP CE+MGYS+PKD+MIFVNVWGIGRDPSIWED +F
Sbjct: 344 LWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSF 403
Query: 301 NPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNG 359
P+RFD G + D KG+DYR+LPFGGGRRICPGLPMA VQVPLILATLIHNFEW LPN
Sbjct: 404 KPERFD---GFDADLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPND 463
BLAST of Chy9G166410 vs. ExPASy TrEMBL
Match:
A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)
HSP 1 Score: 539.7 bits (1389), Expect = 9.6e-150
Identity = 266/390 (68.21%), Postives = 305/390 (78.21%), Query Frame = 0
Query: 1 MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
M PDRALHD SLVFSP+CGD+WKNL+SIC+
Sbjct: 103 MIPDRALHDNCSLVFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQS 162
Query: 61 --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
GS+VEIK+FVF +VFNTLSN I S DL+ FVGDGFNGI+G FK +++ L PNLADFY
Sbjct: 163 KQGSVVEIKEFVFITVFNTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFY 222
Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
PLLR FDLQGLRKKAAIY N++ S W ILIKERR IH + +SDFLDVLIQSGF+D+Q
Sbjct: 223 PLLRCFDLQGLRKKAAIYKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQ 282
Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQY 240
IN LIVE+LSAGTDTTTTTVEWAM+E+LK++DI+N+VREE KK + IDES +S+L Y
Sbjct: 283 INYLIVEVLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHY 342
Query: 241 LWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF 300
LW CVKETLRLHPPAPFLLPR AP CE+MGYS+PK +MI VNVWGIGRDPSIWED Q+F
Sbjct: 343 LWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSF 402
Query: 301 NPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNG 360
P+RFDV C + D KG+DYR+LPFGGGRR+CPGLPMA VQVPLILATLIHNFEW LPN
Sbjct: 403 KPERFDV-C--DADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPND 462
BLAST of Chy9G166410 vs. NCBI nr
Match:
XP_031741123.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])
HSP 1 Score: 675 bits (1741), Expect = 1.09e-241
Identity = 334/391 (85.42%), Postives = 348/391 (89.00%), Query Frame = 0
Query: 1 MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
MTPDRAL++Q SLVFSP+CGD WKNL+SICK
Sbjct: 109 MTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLES 168
Query: 61 --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
GS+V+IKDFVFTSVFNTLSNLI SMDLLDFVGDGFNGIKG FKKMM+LGLTPNLADFY
Sbjct: 169 KQGSVVKIKDFVFTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFY 228
Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
PLLRRFDLQGLRKKAAIYMNEV SLWGILIKERREIHC++GSASSDFLDVLIQSGFSDQQ
Sbjct: 229 PLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQ 288
Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENPIDESNISKLQY 240
IN LI ELLSAGTDTTTTTVEWAMSEILKDK ILNKVR+EIKKVTKE+PIDESNISKLQY
Sbjct: 289 INYLINELLSAGTDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESPIDESNISKLQY 348
Query: 241 LWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF 300
LWSCVKETLRLHPPAPFLLPRLAP DCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF
Sbjct: 349 LWSCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF 408
Query: 301 NPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNG 360
NP+RFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPNG
Sbjct: 409 NPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNG 468
BLAST of Chy9G166410 vs. NCBI nr
Match:
XP_008467223.1 (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])
HSP 1 Score: 674 bits (1740), Expect = 1.54e-241
Identity = 334/393 (84.99%), Postives = 349/393 (88.80%), Query Frame = 0
Query: 1 MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
MTPDRALHDQ SLVFSP+CGDQWKNL+S+CK
Sbjct: 107 MTPDRALHDQHSLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLES 166
Query: 61 --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
GS+VE+KDFVFTSVFNTLSNLI SMDLLDFVGDGF GIKGPFKKMM+LGLTPNLADFY
Sbjct: 167 KQGSVVEVKDFVFTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFY 226
Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
PLLRRFDLQGL+ KA IY NEV SLWGILIKERREIHC++GSAS+DFLDVLIQSGF DQQ
Sbjct: 227 PLLRRFDLQGLKNKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQ 286
Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKEN-PIDESNISKLQ 240
INALI+ELLSAGTDTTTTTVEWAMSEILKDKDILNKVREE+KKVTKEN PIDESNIS+LQ
Sbjct: 287 INALIIELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQ 346
Query: 241 YLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300
YLW CVKETLRLHPPAPFLLPRLAP DCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQT
Sbjct: 347 YLWRCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQT 406
Query: 301 FNPQRFDVGCGN-NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 360
FNPQRFDVGCGN NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP
Sbjct: 407 FNPQRFDVGCGNDNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 466
BLAST of Chy9G166410 vs. NCBI nr
Match:
KAA0055170.1 (putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. makuwa])
HSP 1 Score: 674 bits (1738), Expect = 3.11e-241
Identity = 333/393 (84.73%), Postives = 349/393 (88.80%), Query Frame = 0
Query: 1 MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
MTPDRALHDQ SLVFSP+CGDQWKNL+S+CK
Sbjct: 107 MTPDRALHDQHSLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLES 166
Query: 61 --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
GS+VE+KDFVFTSVFNTLSNLI SMDL+DFVGDGF GIKGPFKKMM+LGLTPNLADFY
Sbjct: 167 KQGSVVEVKDFVFTSVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFY 226
Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
PLLRRFDLQGL+ KA IY NEV SLWGILIKERREIHC++GSAS+DFLDVLIQSGF DQQ
Sbjct: 227 PLLRRFDLQGLKNKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQ 286
Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKEN-PIDESNISKLQ 240
INALI+ELLSAGTDTTTTTVEWAMSEILKDKDILNKVREE+KKVTKEN PIDESNIS+LQ
Sbjct: 287 INALIIELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQ 346
Query: 241 YLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300
YLW CVKETLRLHPPAPFLLPRLAP DCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQT
Sbjct: 347 YLWRCVKETLRLHPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQT 406
Query: 301 FNPQRFDVGCGN-NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 360
FNPQRFDVGCGN NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP
Sbjct: 407 FNPQRFDVGCGNDNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP 466
BLAST of Chy9G166410 vs. NCBI nr
Match:
KAE8647950.1 (hypothetical protein Csa_000545 [Cucumis sativus])
HSP 1 Score: 671 bits (1730), Expect = 5.32e-240
Identity = 333/392 (84.95%), Postives = 347/392 (88.52%), Query Frame = 0
Query: 1 MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
MTPDRAL++Q SLVFSP+CGD WKNL+SICK
Sbjct: 109 MTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLES 168
Query: 61 --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
GS+V+IKDFVFTSVFNTLSNLI SMDLLDFVGDGFNGIKG FKKMM+LGLTPNLADFY
Sbjct: 169 KQGSVVKIKDFVFTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFY 228
Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
PLLRRFDLQGLRKKAAIYMNEV SLWGILIKERREIHC++GSASSDFLDVLIQSGFSDQQ
Sbjct: 229 PLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQ 288
Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENP-IDESNISKLQ 240
IN LI ELL A TDTTTTTVEWAMSEILKDKDILNK REEIKKV KE+P IDESNISKLQ
Sbjct: 289 INYLINELLFAATDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQ 348
Query: 241 YLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300
YLWSCVKETLRLHPP PFLLPRLAP DCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT
Sbjct: 349 YLWSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 408
Query: 301 FNPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPN 360
FNP+RFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN
Sbjct: 409 FNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPN 468
BLAST of Chy9G166410 vs. NCBI nr
Match:
XP_031741076.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])
HSP 1 Score: 658 bits (1697), Expect = 8.04e-235
Identity = 325/392 (82.91%), Postives = 342/392 (87.24%), Query Frame = 0
Query: 1 MTPDRALHDQLSLVFSPQCGDQWKNLKSICK----------------------------- 60
+TPDRALHDQ SLVFS +CGD WK L+SICK
Sbjct: 119 ITPDRALHDQHSLVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVES 178
Query: 61 --GSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFY 120
GS+VEIKDFVFTS FN LSNLI SMDLLDFVGDGFNGIKGPFKKMM+LGLTPNLADFY
Sbjct: 179 KQGSVVEIKDFVFTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFY 238
Query: 121 PLLRRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQQ 180
PLLRRFDLQGL +K IY NEV+S+WGILIKERREI C+KGSAS+DFLDVLIQSGFSDQQ
Sbjct: 239 PLLRRFDLQGLIRKTVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQ 298
Query: 181 INALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKENP-IDESNISKLQ 240
I+ALI+ELL A TDTTTTTVEWAMSEILKDKDILNK REEIKKV KE+P IDESNISKLQ
Sbjct: 299 IDALIIELLFAATDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQ 358
Query: 241 YLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300
YLWSCVKETLRLHPP PFLLPRLAP DCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT
Sbjct: 359 YLWSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 418
Query: 301 FNPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPN 360
FNP+RFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN
Sbjct: 419 FNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPN 478
BLAST of Chy9G166410 vs. TAIR 10
Match:
AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )
HSP 1 Score: 259.6 bits (662), Expect = 3.7e-69
Identity = 133/339 (39.23%), Postives = 208/339 (61.36%), Query Frame = 0
Query: 35 VEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFK--KMMNLGLTPNLADFYPLL 94
+++ + F FN + NL+ S DLLD D G + + K+M PN+ADF+PLL
Sbjct: 175 IDVGRYFFLMAFNLIGNLMFSRDLLD--PDSKRGSEFFYHTGKVMEFAGKPNVADFFPLL 234
Query: 95 RRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGS--ASSDFLDVLIQ-------- 154
R D QG+R+K ++ + + G I+ER E+ ++ S + D+LDVL++
Sbjct: 235 RFLDPQGIRRKTQFHVEKAFEIAGEFIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVD 294
Query: 155 ---SGFSDQQINALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKEN-- 214
S FS + IN ++ E+ +AGTDTTT+T+EWA++E+L + L K++ E++ K +
Sbjct: 295 EEPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQ 354
Query: 215 PIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPTDCEVMG-YSIPKDSMIFVNVWGI 274
+ E ++ L YL + + ETLRLHPP PFL+P A + C + Y+IPK++ + VNVW I
Sbjct: 355 KLQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAI 414
Query: 275 GRDPSIWEDSQTFNPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILA 334
GRDP W D F P+RF + + DFKG DY +LPFG GRR+CP LP+A +PL +
Sbjct: 415 GRDPKTWIDPIMFKPERF-ISDPDARDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIG 474
Query: 335 TLIHNFEWSLPNGEDLSKLDLNGRLGVTLQKYETLKLIP 356
+++ +F+W+L NG + ++D+ R+G+TL+K L+ IP
Sbjct: 475 SMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAIP 510
BLAST of Chy9G166410 vs. TAIR 10
Match:
AT3G52970.2 (cytochrome P450, family 76, subfamily G, polypeptide 1 )
HSP 1 Score: 259.6 bits (662), Expect = 3.7e-69
Identity = 133/339 (39.23%), Postives = 208/339 (61.36%), Query Frame = 0
Query: 35 VEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFK--KMMNLGLTPNLADFYPLL 94
+++ + F FN + NL+ S DLLD D G + + K+M PN+ADF+PLL
Sbjct: 189 IDVGRYFFLMAFNLIGNLMFSRDLLD--PDSKRGSEFFYHTGKVMEFAGKPNVADFFPLL 248
Query: 95 RRFDLQGLRKKAAIYMNEVHSLWGILIKERREIHCKKGS--ASSDFLDVLIQ-------- 154
R D QG+R+K ++ + + G I+ER E+ ++ S + D+LDVL++
Sbjct: 249 RFLDPQGIRRKTQFHVEKAFEIAGEFIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVD 308
Query: 155 ---SGFSDQQINALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKEN-- 214
S FS + IN ++ E+ +AGTDTTT+T+EWA++E+L + L K++ E++ K +
Sbjct: 309 EEPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQ 368
Query: 215 PIDESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPTDCEVMG-YSIPKDSMIFVNVWGI 274
+ E ++ L YL + + ETLRLHPP PFL+P A + C + Y+IPK++ + VNVW I
Sbjct: 369 KLQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAI 428
Query: 275 GRDPSIWEDSQTFNPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILA 334
GRDP W D F P+RF + + DFKG DY +LPFG GRR+CP LP+A +PL +
Sbjct: 429 GRDPKTWIDPIMFKPERF-ISDPDARDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIG 488
Query: 335 TLIHNFEWSLPNGEDLSKLDLNGRLGVTLQKYETLKLIP 356
+++ +F+W+L NG + ++D+ R+G+TL+K L+ IP
Sbjct: 489 SMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAIP 524
BLAST of Chy9G166410 vs. TAIR 10
Match:
AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )
HSP 1 Score: 256.5 bits (654), Expect = 3.1e-68
Identity = 150/377 (39.79%), Postives = 217/377 (57.56%), Query Frame = 0
Query: 14 VFSPQCGDQWKNLK-------------SICKGSIVEIKDFVFTSVFNTLSNLILSMDLLD 73
+FSPQC K L+ S + V+I F + N +SN++ S++L
Sbjct: 142 LFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISHVSFVTALNIISNILFSVNLGS 201
Query: 74 FVGDGFNGIKGPFKKMM-----NLGLTPNLADFYPLLRRFDLQG----LRKKAAIYMNEV 133
+ D N F++M+ ++G P+LA+F+P +R DLQG +R+ + +
Sbjct: 202 Y--DSKN--SSAFQEMVIGYQESIG-NPDLANFFPFMRFLDLQGNSKKMRESSGRLLQVF 261
Query: 134 HSLWGILIKERREIHCKKGSASSDFLDVLIQSGFSDQ------QINALIVELLSAGTDTT 193
+ I E+ +K +S DFLDVLI D+ +I L++++ AGTDT
Sbjct: 262 REFYDARIVEKSSRSVEKDVSSKDFLDVLIDLQQGDETEINIDEIEHLLLDMFVAGTDTN 321
Query: 194 TTTVEWAMSEILKDKDILNKVREEIKKVTKEN-PIDESNISKLQYLWSCVKETLRLHPPA 253
++TVEWAM+E+L + + KV++EI V +N ES+ISKL YL + VKET RLHP A
Sbjct: 322 SSTVEWAMAELLGNPKTMTKVQDEINHVIGQNGDFQESDISKLPYLKAVVKETFRLHPAA 381
Query: 254 PFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQRFDVGCGNNVDF 313
PFLL R A T+ E++G+++ KDS + VNVW IGRDP +WE+ F P+RF G +D
Sbjct: 382 PFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWENPTHFEPERF---LGKEIDV 441
Query: 314 KGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDLSKLDLNGRLGVT 361
KG DY PFG GRRICPGLP+A+ V L+LA+L++ FEW LPNG LD+ G+T
Sbjct: 442 KGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLPNGVGSEDLDMEETFGLT 501
BLAST of Chy9G166410 vs. TAIR 10
Match:
AT1G33730.1 (cytochrome P450, family 76, subfamily C, polypeptide 5 )
HSP 1 Score: 255.8 bits (652), Expect = 5.3e-68
Identity = 133/332 (40.06%), Postives = 199/332 (59.94%), Query Frame = 0
Query: 31 KGSIVEIKDFVFTSVFNTLSNLILSMDLLDFVGDGFNGIKGPFKKMMNLGLTPNLADFYP 90
+G V+I F + N +SN++ S++L + + + M P++++F+P
Sbjct: 36 RGEAVDISRASFVTALNIISNILFSVNLGSYDSKNSSAFQEMVIGYMESIGNPDVSNFFP 95
Query: 91 LLRRFDLQGLRKKAAIYMNEV----HSLWGILIKERREIHCKKGSASSDFLDVLI----- 150
+R DLQG KK Y ++ + I E +K +S DFLD LI
Sbjct: 96 FMRLLDLQGNSKKMKEYSGKLLQVFREFYDARILENSSRIDEKDVSSRDFLDALIDLQQG 155
Query: 151 -QSGFSDQQINALIVELLSAGTDTTTTTVEWAMSEILKDKDILNKVREEIKKVTKEN-PI 210
+S + +I L++++ AGTDT ++TVEWAM+E+L + + KV++EI +V ++N +
Sbjct: 156 DESEINIDEIEHLLLDMFLAGTDTNSSTVEWAMTELLGNPKTMTKVQDEINRVIRQNGDV 215
Query: 211 DESNISKLQYLWSCVKETLRLHPPAPFLLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRD 270
ES+ISKL YL + +KET RLHP APFLLPR A D +++G+ +PKDS + VNVW IGRD
Sbjct: 216 QESHISKLPYLQAVIKETFRLHPAAPFLLPRKAERDVDILGFHVPKDSHVLVNVWAIGRD 275
Query: 271 PSIWEDSQTFNPQRFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLI 330
P++WE+ F P+RF G ++D KG +Y PFG GRRICPGLP+A+ V L+LA+L+
Sbjct: 276 PNVWENPTQFEPERF---LGKDIDVKGTNYELTPFGAGRRICPGLPLALKTVHLMLASLL 335
Query: 331 HNFEWSLPNGEDLSKLDLNGRLGVTLQKYETL 352
+ FEW LPNG LD+ G+T+ K L
Sbjct: 336 YTFEWKLPNGVGSEDLDMGETFGLTVHKTNPL 364
BLAST of Chy9G166410 vs. TAIR 10
Match:
AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )
HSP 1 Score: 255.8 bits (652), Expect = 5.3e-68
Identity = 140/372 (37.63%), Postives = 219/372 (58.87%), Query Frame = 0
Query: 11 LSLVFSPQCGDQWKNLK-------------SICKGSIVEIKDFVFTSVFNTLSNLILSMD 70
++L+FSPQ + K L+ S + V+I +T+V N +SN++ S+D
Sbjct: 139 VTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNIISNILFSVD 198
Query: 71 LLDFVGDGFNGIKGPFKKMMNLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVHSLW-G 130
L + N + M P+ A+++P + DLQG RK + ++ G
Sbjct: 199 LGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTERLFRVFRG 258
Query: 131 IL---IKERREIHCKKGSASSDFLDVLI------QSGFSDQQINALIVELLSAGTDTTTT 190
+ I E+ + K ++ DFLD L+ ++ + I L++++ +AGTDT+++
Sbjct: 259 FMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLLLDMFTAGTDTSSS 318
Query: 191 TVEWAMSEILKDKDILNKVREEIKKVTKENP-IDESNISKLQYLWSCVKETLRLHPPAPF 250
T+EWAM+E+L++ + K + E+ +V +N + ES+IS L YL + VKET RLHP AP
Sbjct: 319 TLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETFRLHPAAPL 378
Query: 251 LLPRLAPTDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPQRFDVGCGNNVDFKG 310
L+PR A +D EV+G+ +PKD+ + VNVW IGRDPS+WE+ F P+RF G ++D KG
Sbjct: 379 LVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERF---MGKDIDVKG 438
Query: 311 YDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNGEDLSKLDLNGRLGVTLQ 359
DY PFGGGRRICPGLP+A+ V L+LA+L+++F+W LPNG LD++ G+TL
Sbjct: 439 RDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITLH 498
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9FXW4 | 1.3e-82 | 41.30 | Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3... | [more] |
O64899 | 2.4e-81 | 40.57 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif... | [more] |
O64900 | 2.7e-80 | 40.16 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3... | [more] |
Q9SP06 | 4.5e-80 | 39.22 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum... | [more] |
A8CDR5 | 3.0e-76 | 39.32 | Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3CT14 | 1.1e-190 | 84.99 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656... | [more] |
A0A5A7UIU3 | 1.9e-190 | 84.73 | Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma... | [more] |
A0A6J1ELA6 | 1.2e-152 | 69.92 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... | [more] |
A0A6J1I4V7 | 3.0e-151 | 68.89 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=... | [more] |
A0A6J1ER52 | 9.6e-150 | 68.21 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... | [more] |
Match Name | E-value | Identity | Description | |
XP_031741123.1 | 1.09e-241 | 85.42 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus] | [more] |
XP_008467223.1 | 1.54e-241 | 84.99 | PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis mel... | [more] |
KAA0055170.1 | 3.11e-241 | 84.73 | putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. maku... | [more] |
KAE8647950.1 | 5.32e-240 | 84.95 | hypothetical protein Csa_000545 [Cucumis sativus] | [more] |
XP_031741076.1 | 8.04e-235 | 82.91 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus] | [more] |
Match Name | E-value | Identity | Description | |
AT3G52970.1 | 3.7e-69 | 39.23 | cytochrome P450, family 76, subfamily G, polypeptide 1 | [more] |
AT3G52970.2 | 3.7e-69 | 39.23 | cytochrome P450, family 76, subfamily G, polypeptide 1 | [more] |
AT1G33720.1 | 3.1e-68 | 39.79 | cytochrome P450, family 76, subfamily C, polypeptide 6 | [more] |
AT1G33730.1 | 5.3e-68 | 40.06 | cytochrome P450, family 76, subfamily C, polypeptide 5 | [more] |
AT2G45550.1 | 5.3e-68 | 37.63 | cytochrome P450, family 76, subfamily C, polypeptide 4 | [more] |