Chy9G164020 (gene) Cucumber (hystrix) v1

Overview


Name	Chy9G164020
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Pectinesterase
Location	chrH09: 9034791 .. 9037345 (-)
RNA-Seq Expression	Chy9G164020
Synteny	Chy9G164020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCATCCATCCATTTGGATGGAAGAGAGCCACAGACAGGAGTTCAACTGTTTTTGTCTTTTCCTCCTTTTTTAAGGTATTTTTTCTTTGTTTTAGTATGTTAATTTTAAACAAGAAAAATCAAATTCTTATGCTCTCAATAAAGTAAAATAGTGTGCATTATTATTATCCTGCCATATATCTTTTTTAAACACACATTTTAACACACATGTATATACAACGAACTGTATTTTCCATATATACTGTATGCAGTTTTGTCCTCCAAATATAAATTCTTTAAAAAGTGTGCATCTTTATAACCTTTCCATTGATATGTATTTGTAAATTTTTTATAGACATATCGACCAAATATATGTATTACATTATGCTAAAGTAATAATGATACACAAACTATATATATATTGTGATTACAAACAACAATAAATGAGAGACAAAAATCTAATGAAAATTGACAGCAAAATGAAGATGGTGAACAATAGTAAAAAGGAGTTACTAGAATCTAAAAAAATGGTAAAAAAAAAAAAAGATAAACAAAATACAATAATGGAGAAGACAAACAAATGTGCAAGAGATGACTGAAATTGGGTAAATGGAGAAGAAATGTGAGTTCAAGTAAAATATAAATGGAGAAAAGATTAAATTGTCAACTTAAGAAAAAAGTGGGGAAAATATTAAATGCATTTTATGCAAAAAACTGAAATTTTTTCTGCCTTTCTTCCATAGCGTTTTAAAAAGAGGCTATTTCTTGTAGTTATTTTGTAGTGGAAACAAATTGTGTGAAGTAAAGTCACTAAAATTGTGATAATTTCCAAAATAATAACAAAAAAACCACAAAATGTGGGTAATTTTTTCAATTAAAAAGAACATTTAGAGGAGAAAATGGTAAGAAAACATACCTAAATGAAAATAAAAATAAACTGTTGTGAAAAATAACTATTCTTAACACATAATAGTTAATTTATTTTAATAATAATGAACCAAATTTAATAGAAAAATGCTGTTTAATGACATATTCATTAATTTTGATGATTTAAATAAAAAAAAATGTATCCATTGATTGCATGCGTAAATCCTCGATCTAGCCTTTTTTTTCCTTTTAAAGATATATTTTAGTTTACTACATCAATTAGTATTAAACCAAATGAATTTTTAATGAACCATCCATTCTCTATAAATACAAGGGCTTCCTAGTCCCTATTTTCACATATCCTTTTTGCATTCAATAATATTTTGTCTATTACTTTCAACTTAACAAACATGACAATATTCACACTTAAAATGCTCTTTATAGTTTATTTGTTAGTTGTTGTTATTGTTGAGTTTTCTGATTGCTTTAGTTTCAATTTCACAGTGTCTTTAGATGGTACAGGAAATTTTGTTAAAATTAACGATGCTATAGCAGCAGCACCCAATTTTAGTATGACAAGATTTTACATTCATGTAAAACCTGGAACTTACAAGGAGATTATTGAAGTTCCATATGAGAAAACTTGTATTGCTTTAATTGGAGATGATTCTTCTACTACCATCATCGTTAACAATCGAAGTAATGGCACTGGATCCTCCACGGCATCTTCTGCGACTCTCAGTAAGCTTCAATTATCCCAACCCTCATTTTAACAAGGGCTTATTTGGTAATTATTTTTTTTGTTTTTAATTTTTAAAAATTAGATCTATATATAAACACCATTTTACCTTTAATTTTCTTGTTTTATTTAAAAAACCATTCGTTTTCAAACACTTGAAAATTAAACTCTCTTAAGAAAACAACAGGTAAGAAGTTGAAGAAAAAAATATAGGTTTAATTTTTAAAAATCAAATTGTTAGTAAATTTTATAATTTATTACTCAAACAATGTTTGATTTTGGAAGCTGTAAACGGAGCAAACTTCATGGCTCAGTTCTTGACCTTTCAAAACACTGCTGGACCAAATGAAGGCCAAGCAATTGCTCTTCTTGACCAAGCAACACACACAGCTTACTACAAGTGTGTGTTTTTGGGTTATCAAGATACTCTCTATGCTGGAGCACTTCCCCAGTTCTTCAAAGAATGCGATATCTATGGAAGCGTAGACTTCATTTTTGGAAATGGGCTTGTTGTATTCCAAGACTGTAATATATACGCTCGATTATTTGACGTTCAAATTACCGTAACAGCACAATCGAAACCAAGTTTAAGTGCTCTGAGCGGCTTCATCTTCCAAAATTGCAACGTGACTGTGTCGCCAGAGATAGCATCAAGTAAGGACAAGGTAACGGTGTTTCTTGGACGACCATGGAGAATGTACTCTATGGTTGTTTTCATTGATTCATTTCTTGACAATGTGGTTCAACCTAAAGGTTGGTTGGAGTGGCCGGGAGTGCCAGAGAATTTATTGTATTATGCAGAATACAATAACAGTGGTGGAGGAGCCAACACTTCTCAAAGGGTTAATTGGCCTGGTTTTCATGTGTTAAATAACGTAAAAGAAGTTGCAACTTTTACTGTTGAAATATTTATTAATGGGACTCAATGGTTGCCTCAAACTGGCATACCATTTAGAGCTGGTTTTTAG

mRNA sequence

ATGCCATCCATCCATTTGGATGGAAGAGAGCCACAGACAGGAGTTCAACTGTTTTTGTCTTTTCCTCCTTTTTTAAGTTTCAATTTCACAGTGTCTTTAGATGGTACAGGAAATTTTGTTAAAATTAACGATGCTATAGCAGCAGCACCCAATTTTAGTATGACAAGATTTTACATTCATGTAAAACCTGGAACTTACAAGGAGATTATTGAAGTTCCATATGAGAAAACTTGTATTGCTTTAATTGGAGATGATTCTTCTACTACCATCATCGTTAACAATCGAAGTAATGGCACTGGATCCTCCACGGCATCTTCTGCGACTCTCACTGTAAACGGAGCAAACTTCATGGCTCAGTTCTTGACCTTTCAAAACACTGCTGGACCAAATGAAGGCCAAGCAATTGCTCTTCTTGACCAAGCAACACACACAGCTTACTACAAGTGTGTGTTTTTGGGTTATCAAGATACTCTCTATGCTGGAGCACTTCCCCAGTTCTTCAAAGAATGCGATATCTATGGAAGCGTAGACTTCATTTTTGGAAATGGGCTTGTTGTATTCCAAGACTGTAATATATACGCTCGATTATTTGACGTTCAAATTACCGTAACAGCACAATCGAAACCAAGTTTAAGTGCTCTGAGCGGCTTCATCTTCCAAAATTGCAACGTGACTGTGTCGCCAGAGATAGCATCAAGTAAGGACAAGGTAACGGTGTTTCTTGGACGACCATGGAGAATGTACTCTATGGTTGTTTTCATTGATTCATTTCTTGACAATGTGGTTCAACCTAAAGGTTGGTTGGAGTGGCCGGGAGTGCCAGAGAATTTATTGTATTATGCAGAATACAATAACAGTGGTGGAGGAGCCAACACTTCTCAAAGGGTTAATTGGCCTGGTTTTCATGTGTTAAATAACGTAAAAGAAGTTGCAACTTTTACTGTTGAAATATTTATTAATGGGACTCAATGGTTGCCTCAAACTGGCATACCATTTAGAGCTGGTTTTTAG

Coding sequence (CDS)

ATGCCATCCATCCATTTGGATGGAAGAGAGCCACAGACAGGAGTTCAACTGTTTTTGTCTTTTCCTCCTTTTTTAAGTTTCAATTTCACAGTGTCTTTAGATGGTACAGGAAATTTTGTTAAAATTAACGATGCTATAGCAGCAGCACCCAATTTTAGTATGACAAGATTTTACATTCATGTAAAACCTGGAACTTACAAGGAGATTATTGAAGTTCCATATGAGAAAACTTGTATTGCTTTAATTGGAGATGATTCTTCTACTACCATCATCGTTAACAATCGAAGTAATGGCACTGGATCCTCCACGGCATCTTCTGCGACTCTCACTGTAAACGGAGCAAACTTCATGGCTCAGTTCTTGACCTTTCAAAACACTGCTGGACCAAATGAAGGCCAAGCAATTGCTCTTCTTGACCAAGCAACACACACAGCTTACTACAAGTGTGTGTTTTTGGGTTATCAAGATACTCTCTATGCTGGAGCACTTCCCCAGTTCTTCAAAGAATGCGATATCTATGGAAGCGTAGACTTCATTTTTGGAAATGGGCTTGTTGTATTCCAAGACTGTAATATATACGCTCGATTATTTGACGTTCAAATTACCGTAACAGCACAATCGAAACCAAGTTTAAGTGCTCTGAGCGGCTTCATCTTCCAAAATTGCAACGTGACTGTGTCGCCAGAGATAGCATCAAGTAAGGACAAGGTAACGGTGTTTCTTGGACGACCATGGAGAATGTACTCTATGGTTGTTTTCATTGATTCATTTCTTGACAATGTGGTTCAACCTAAAGGTTGGTTGGAGTGGCCGGGAGTGCCAGAGAATTTATTGTATTATGCAGAATACAATAACAGTGGTGGAGGAGCCAACACTTCTCAAAGGGTTAATTGGCCTGGTTTTCATGTGTTAAATAACGTAAAAGAAGTTGCAACTTTTACTGTTGAAATATTTATTAATGGGACTCAATGGTTGCCTCAAACTGGCATACCATTTAGAGCTGGTTTTTAG

Protein sequence

MPSIHLDGREPQTGVQLFLSFPPFLSFNFTVSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGTQWLPQTGIPFRAGF*

Homology

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BLAST of Chy9G164020 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.2e-79
Identity = 147/314 (46.82%), Postives = 200/314 (63.69%), Query Frame = 0


Query: 26  SFNFTVSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDD 85
           ++N +V++DGTGNF  INDA+ AAPN S TRF I++K G Y E +E+P +KT I  IGD 
Sbjct: 219 NYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDG 278

Query: 86  SSTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTA 145
              T+I  NRS   G ST  + T+ V G  ++A+ ++F N+AGP + QA+A    + H+A
Sbjct: 279 IGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSA 338

Query: 146 YYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDV--QITV 205
           +Y+C F GYQDTLY  +  QF++ECDIYG++DFIFGN  VVFQ+ ++YAR  +   +I  
Sbjct: 339 FYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAF 398

Query: 206 TAQSKPSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQ 265
           TAQS+      +G    NC +  +P++   K+    +LGRPWR YS  V I SF+D+++ 
Sbjct: 399 TAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIH 458

Query: 266 PKGWLEW-PGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGT 325
           P GWLE         LYY EY N G GAN ++RV WPGF  + N  E   FTV  FI+G+
Sbjct: 459 PAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGS 518

Query: 326 QWLPQTGIPFRAGF 337
            WL  TGIPF  GF
Sbjct: 519 TWLNSTGIPFSLGF 532

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BLAST of Chy9G164020 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 6.8e-78
Identity = 145/310 (46.77%), Postives = 201/310 (64.84%), Query Frame = 0


Query: 28  NFTVSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDDSS 87
           N  V+ DG+GN   I +A+AAA     +R+ I++K GTY E IEV  +   I  +GD   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGIG 261

Query: 88  TTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTAYY 147
            TII  ++S G G++T  SAT+ V G NF+A+ +T +NTAGPN  QA+AL   +  + +Y
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFY 321

Query: 148 KCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQI-TVTAQ 207
           +C F GYQDTLY  +  QF++ECDIYG+VDFIFGN  VV Q+CNI+AR    +  T+TAQ
Sbjct: 322 RCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQ 381

Query: 208 SKPSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKG 267
            +   +  +G I  NC VT + ++   +  V  FLGRPW+ YS  V+I +FLD+++ P G
Sbjct: 382 GRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAG 441

Query: 268 WLEWPG-VPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGTQWL 327
           W+EW G    N LYYAEY N+G G++T+ RV W G+HVL +  +V+ FTV  FI G  WL
Sbjct: 442 WMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWL 501

Query: 328 PQTGIPFRAG 336
           P T +PF +G
Sbjct: 502 PATNVPFTSG 509

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BLAST of Chy9G164020 vs. ExPASy Swiss-Prot
Match: O48711 (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PME12 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 9.8e-77
Identity = 148/313 (47.28%), Postives = 197/313 (62.94%), Query Frame = 0


Query: 26  SFNFTVSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDD 85
           S +  V+ DGTGNF  IN+AI+ APN S  R  I+VK G Y E I++P  KT I LIGD 
Sbjct: 235 SESLVVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDG 294

Query: 86  SSTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTA 145
           S  T I  NRS G G +T  SATL V+G  F+A+ +   NTAGP + QA+AL   A   A
Sbjct: 295 SDVTFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVA 354

Query: 146 YYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARL-FDVQITV- 205
            Y+CV  GYQDTLY  +  QF++ECDIYG++D+IFGN  VVFQ CNI ++L    Q TV 
Sbjct: 355 LYRCVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVI 414

Query: 206 TAQSKPSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQ 265
           TAQS+ +    +G   QNC++  S ++ +S +KV  +LGRPWR +S  V ++S++D  + 
Sbjct: 415 TAQSRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFID 474

Query: 266 PKGWLEWPGVPE-NLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGT 325
             GW +W G    + LYY EYNN+G G+ T +RVNWPGFH++   ++   FT   FI G 
Sbjct: 475 GSGWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIM-GYEDAFNFTATEFITGD 534

Query: 326 QWLPQTGIPFRAG 336
            WL  T  P+  G
Sbjct: 535 GWLGSTSFPYDNG 546

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BLAST of Chy9G164020 vs. ExPASy Swiss-Prot
Match: Q9SMY7 (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3702 GN=PME44 PE=2 SV=2)

HSP 1 Score: 285.8 bits (730), Expect = 6.3e-76
Identity = 140/313 (44.73%), Postives = 200/313 (63.90%), Query Frame = 0


Query: 26  SFNFTVSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDD 85
           +++ +V+LDGTGNF KI DAI  AP++S TRF I++K G Y E +E+  +K  I ++GD 
Sbjct: 210 TYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDG 269

Query: 86  SSTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTA 145
              T+I  NRS   G +T  SAT  V+G  F+A+ +TFQNTAGP + QA+AL   +  + 
Sbjct: 270 IDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSV 329

Query: 146 YYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYAR--LFDVQITV 205
           +++C   GYQDTLY   + QF++EC I G+VDFIFG+G VVFQ+C I A+  L + + T+
Sbjct: 330 FFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNTI 389

Query: 206 TAQSKPSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQ 265
           TAQ +  ++  SGF  Q  N++   ++    +    +LGRPW++YS  VFI + + +VV+
Sbjct: 390 TAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVVR 449

Query: 266 PKGWLEW-PGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGT 325
           P+GWLEW      + L+Y E+ N G G+  S RV WPG+HV NN  +   FTV  FI G 
Sbjct: 450 PEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKGN 509

Query: 326 QWLPQTGIPFRAG 336
            WLP TG+ F  G
Sbjct: 510 LWLPSTGVTFSDG 522

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BLAST of Chy9G164020 vs. ExPASy Swiss-Prot
Match: Q43062 (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 1.8e-75
Identity = 140/316 (44.30%), Postives = 200/316 (63.29%), Query Frame = 0


Query: 25  LSFNFTVSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGD 84
           +S +  V+ DGTGNF  + DA+ AAP++SM R+ I++K GTYKE +E+  +K  + +IGD
Sbjct: 205 VSVDAIVAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGD 264

Query: 85  DSSTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHT 144
               TII  NRS   G +T  SAT  V+G  F+A+ +TF+NTAGP + QA+AL   +  +
Sbjct: 265 GMDATIISGNRSFVDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLS 324

Query: 145 AYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYAR--LFDVQIT 204
            +Y+C   GYQDTLY   + QF+++C I G+VDFIFG+  VVFQ+C I A+  L + + +
Sbjct: 325 VFYRCNIRGYQDTLYTHTMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNS 384

Query: 205 VTAQSKPSLSALSGFIFQNCNVTVSPEI-ASSKDKVTVFLGRPWRMYSMVVFIDSFLDNV 264
           +TAQ +   +  +G   Q CN+T   ++ A+S +    +LGRPW++YS  V + SFL NV
Sbjct: 385 ITAQGRKDPNEPTGISIQFCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNV 444

Query: 265 VQPKGWLEWPG-VPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFIN 324
           ++P+GWLEW G    N L+Y EY N G GA    RV WPG+ V N   +   +TV  FI 
Sbjct: 445 IRPEGWLEWNGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIE 504

Query: 325 GTQWLPQTGIPFRAGF 337
           G  WLP TG+ + A F
Sbjct: 505 GNLWLPSTGVKYTAEF 520

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BLAST of Chy9G164020 vs. ExPASy TrEMBL
Match: A0A0A0L1M3 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.1e-174
Identity = 301/322 (93.48%), Postives = 309/322 (95.96%), Query Frame = 0


Query: 15  VQLFLSFPPFLSFNFTVSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPY 74
           V + + F    SFN TVSLDGT NF+KINDAIAAAPNFS TRFYIHVKPGTY+EIIEVPY
Sbjct: 9   VVVIVEFSDCFSFNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPY 68

Query: 75  EKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQA 134
           EKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNG+NFMAQFLTFQNTAGPNEGQA
Sbjct: 69  EKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQA 128

Query: 135 IALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYA 194
           IALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYA
Sbjct: 129 IALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYA 188

Query: 195 RLFDVQITVTAQSKPSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFI 254
           RLFDVQITVTAQSKPSL++LSGFIFQNC VTVSPEIASSKDKVTVFLGRPWRMYSMVVFI
Sbjct: 189 RLFDVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWRMYSMVVFI 248

Query: 255 DSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFT 314
           DSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPG+H LNN KEVATFT
Sbjct: 249 DSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFT 308

Query: 315 VEIFINGTQWLPQTGIPFRAGF 337
           VEIFINGTQWLPQTGIPFRAGF
Sbjct: 309 VEIFINGTQWLPQTGIPFRAGF 330

[Close]

BLAST of Chy9G164020 vs. ExPASy TrEMBL
Match: A0A1S3CQ52 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503419 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.9e-164
Identity = 283/306 (92.48%), Postives = 298/306 (97.39%), Query Frame = 0


Query: 31  VSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDDSSTTI 90
           +SLDGTGNFVK+NDAIAAAPNFSMTRFYIHVKPGTY+EIIEVP EKT IALIGDDSSTTI
Sbjct: 19  LSLDGTGNFVKVNDAIAAAPNFSMTRFYIHVKPGTYQEIIEVPSEKTFIALIGDDSSTTI 78

Query: 91  IVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 150
           IVNNRSNGTGSSTASSATLTV G NFMAQFLTFQN+AGPNEGQAIA+LDQA HTAYY+CV
Sbjct: 79  IVNNRSNGTGSSTASSATLTVYGENFMAQFLTFQNSAGPNEGQAIAVLDQAKHTAYYRCV 138

Query: 151 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 210
           FLGYQDTLYAG++PQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK S
Sbjct: 139 FLGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKQS 198

Query: 211 LSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 270
           LSA SGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLD+VVQPKGWLEW
Sbjct: 199 LSAPSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWLEW 258

Query: 271 PGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGTQWLPQTGI 330
           PGVPEN+LYYAEYNNSGGGANTSQRVNWPG+HVL+N +EVATF+VE+FINGTQWLPQTGI
Sbjct: 259 PGVPENVLYYAEYNNSGGGANTSQRVNWPGYHVLDNAREVATFSVEMFINGTQWLPQTGI 318

Query: 331 PFRAGF 337
           PFRAGF
Sbjct: 319 PFRAGF 324

[Close]

BLAST of Chy9G164020 vs. ExPASy TrEMBL
Match: A0A5D3C2R6 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00440 PE=4 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 6.4e-164
Identity = 283/306 (92.48%), Postives = 298/306 (97.39%), Query Frame = 0


Query: 31  VSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDDSSTTI 90
           VSLDGTGNFVK+NDAIAAAPNFSMTRFYIHVKPGTY+EIIEVP EKT IALIGDDSSTTI
Sbjct: 12  VSLDGTGNFVKVNDAIAAAPNFSMTRFYIHVKPGTYQEIIEVPSEKTFIALIGDDSSTTI 71

Query: 91  IVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 150
           IVNNRSNGTGSSTASSATLTV G NFMA+FLTFQN+AGPNEGQAIA+LDQA HTAYY+CV
Sbjct: 72  IVNNRSNGTGSSTASSATLTVYGENFMARFLTFQNSAGPNEGQAIAVLDQAKHTAYYRCV 131

Query: 151 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 210
           FLGYQDTLYAG++PQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK S
Sbjct: 132 FLGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKQS 191

Query: 211 LSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 270
           LSA SGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLD+VVQPKGWLEW
Sbjct: 192 LSAPSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWLEW 251

Query: 271 PGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGTQWLPQTGI 330
           PGVPEN+LYYAEYNNSGGGANTSQRVNWPG+HVL+N +EVATF+VE+FINGTQWLPQTGI
Sbjct: 252 PGVPENVLYYAEYNNSGGGANTSQRVNWPGYHVLDNAREVATFSVEMFINGTQWLPQTGI 311

Query: 331 PFRAGF 337
           PFRAGF
Sbjct: 312 PFRAGF 317

[Close]

BLAST of Chy9G164020 vs. ExPASy TrEMBL
Match: A0A6J1KPG6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496427 PE=4 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 8.7e-145
Identity = 250/318 (78.62%), Postives = 278/318 (87.42%), Query Frame = 0


Query: 18  FLSFPPFLSFNFTVSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKT 77
           FL F     FN TVSLDGTGNFVKI+DAI AAPNF+ TRFYIH+KPG Y E IE+P +KT
Sbjct: 18  FLKFSSTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFYIHIKPGIYNEHIEIPSQKT 77

Query: 78  CIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIAL 137
            I+LIGD++STTIIV+NRS  TG STA+SATLTVNGANFMA+FLTFQN+AGP  GQA+A+
Sbjct: 78  FISLIGDNASTTIIVDNRSKATGFSTANSATLTVNGANFMAKFLTFQNSAGPKMGQAVAV 137

Query: 138 LDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLF 197
           LDQA HTAYYKCVFLGYQDTLYA A+PQFFKECDIYG+VDFIFGNGLVVFQDCNIYARL 
Sbjct: 138 LDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVVFQDCNIYARLV 197

Query: 198 DVQITVTAQSKPSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSF 257
           D+Q TVTAQSK +L   SGF+FQNC VTVSPEIASS+DKV VFLGRPWR YSMV F++SF
Sbjct: 198 DIQSTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRPWRAYSMVAFVESF 257

Query: 258 LDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEI 317
           LDNVVQPKGWLEW GVP NLLYY EYNN GGGANTSQRV+WPGFHVL+++ +VA FTVE 
Sbjct: 258 LDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVLHSLTDVAKFTVEN 317

Query: 318 FINGTQWLPQTGIPFRAG 336
           FINGT WLPQT IPFR+G
Sbjct: 318 FINGTTWLPQTTIPFRSG 335

[Close]

BLAST of Chy9G164020 vs. ExPASy TrEMBL
Match: A0A6J1GIG0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454459 PE=4 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 4.3e-144
Identity = 248/318 (77.99%), Postives = 277/318 (87.11%), Query Frame = 0


Query: 18  FLSFPPFLSFNFTVSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKT 77
           FL F     FN TVSLDGTGNFVKI+DAI AAPNF+ TRFYIH+KPG Y E IEVP++KT
Sbjct: 18  FLKFSNTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFYIHIKPGIYNEHIEVPFQKT 77

Query: 78  CIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIAL 137
            I+LIGD++STTIIV+NRS   G ST++SATLTVNGANFMA+FLTFQN+AGP  GQA+A+
Sbjct: 78  FISLIGDNASTTIIVDNRSKAAGFSTSNSATLTVNGANFMAKFLTFQNSAGPKMGQAVAV 137

Query: 138 LDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLF 197
           LDQA HTAYYKCVFLGYQDTLYA A+PQFFKECDIYG+VDFIFGNGLVVFQDCNIYARL 
Sbjct: 138 LDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVVFQDCNIYARLV 197

Query: 198 DVQITVTAQSKPSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSF 257
           D+  TVTAQSK +L   SGF+FQNC VTVSPEIASS+DKV VFLGRPWR YSMV F++SF
Sbjct: 198 DIHTTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRPWRAYSMVAFVESF 257

Query: 258 LDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEI 317
           LDNVVQPKGWLEW GVP NLLYY EYNN GGGANTSQRV+WPGFHVL+++ +VA FTVE 
Sbjct: 258 LDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVLHSLTDVANFTVEN 317

Query: 318 FINGTQWLPQTGIPFRAG 336
           FINGT WLP+T IPFRAG
Sbjct: 318 FINGTTWLPETTIPFRAG 335

[Close]

BLAST of Chy9G164020 vs. NCBI nr
Match: XP_011654361.2 (probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 613 bits (1582), Expect = 1.76e-220
Identity = 299/322 (92.86%), Postives = 307/322 (95.34%), Query Frame = 0


Query: 15  VQLFLSFPPFLSFNFTVSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPY 74
           V + + F    SFN TVSLDGT NF+KINDAIAAAPNFS TRFYIHVKPGTY+EIIEVPY
Sbjct: 16  VVVIVEFSDCFSFNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPY 75

Query: 75  EKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQA 134
           EKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNG+NFMAQFLTFQNTAGPNEGQA
Sbjct: 76  EKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNTAGPNEGQA 135

Query: 135 IALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYA 194
           IALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYA
Sbjct: 136 IALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYA 195

Query: 195 RLFDVQITVTAQSKPSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFI 254
           RLFDVQITVTAQSKPSL++LSGFIFQNCNVTVSPEIA SKD VTVFLGRPWRMYSMVVFI
Sbjct: 196 RLFDVQITVTAQSKPSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFI 255

Query: 255 DSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFT 314
           DSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPG+H LNN KEVATFT
Sbjct: 256 DSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFT 315

Query: 315 VEIFINGTQWLPQTGIPFRAGF 336
           VE FINGTQWLPQTGIPFRAGF
Sbjct: 316 VETFINGTQWLPQTGIPFRAGF 337

[Close]

BLAST of Chy9G164020 vs. NCBI nr
Match: XP_031741746.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus] >KAE8648510.1 hypothetical protein Csa_009140 [Cucumis sativus])

HSP 1 Score: 612 bits (1577), Expect = 3.73e-220
Identity = 298/306 (97.39%), Postives = 302/306 (98.69%), Query Frame = 0


Query: 31  VSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDDSSTTI 90
           +SLDGTGNFVKINDAIAAAPNFS TRFYIHVKPGTYKEIIEVPYEKTCIALIGDD+STTI
Sbjct: 5   LSLDGTGNFVKINDAIAAAPNFSTTRFYIHVKPGTYKEIIEVPYEKTCIALIGDDASTTI 64

Query: 91  IVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 150
           IVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIA+LDQA HTAYYKCV
Sbjct: 65  IVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIAVLDQAKHTAYYKCV 124

Query: 151 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 210
           FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS
Sbjct: 125 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 184

Query: 211 LSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 270
           LSALSGFIFQNC VTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW
Sbjct: 185 LSALSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 244

Query: 271 PGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGTQWLPQTGI 330
           PGVPENLLYYAEYNNSGGGANTSQRVNWPG+HVLNN KEVATFTVEIFINGTQWLPQTGI
Sbjct: 245 PGVPENLLYYAEYNNSGGGANTSQRVNWPGYHVLNNAKEVATFTVEIFINGTQWLPQTGI 304

Query: 331 PFRAGF 336
           PFRAGF
Sbjct: 305 PFRAGF 310

[Close]

BLAST of Chy9G164020 vs. NCBI nr
Match: XP_031745525.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 599 bits (1545), Expect = 7.67e-215
Identity = 293/322 (90.99%), Postives = 300/322 (93.17%), Query Frame = 0


Query: 15  VQLFLSFPPFLSFNFTVSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPY 74
           V + + F    SFN TVSLDGT NF+KINDAIAAAPNFS TRFYIHVKPGTY+EIIEVPY
Sbjct: 16  VVVIVEFSDCFSFNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTYQEIIEVPY 75

Query: 75  EKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQA 134
           EKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNG       LTFQNTAGPNEGQA
Sbjct: 76  EKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGIKLHGSVLTFQNTAGPNEGQA 135

Query: 135 IALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYA 194
           IALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYA
Sbjct: 136 IALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYA 195

Query: 195 RLFDVQITVTAQSKPSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFI 254
           RLFDVQITVTAQSKPSL++LSGFIFQNCNVTVSPEIA SKD VTVFLGRPWRMYSMVVFI
Sbjct: 196 RLFDVQITVTAQSKPSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVFLGRPWRMYSMVVFI 255

Query: 255 DSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFT 314
           DSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPG+H LNN KEVATFT
Sbjct: 256 DSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPGYHALNNAKEVATFT 315

Query: 315 VEIFINGTQWLPQTGIPFRAGF 336
           VE FINGTQWLPQTGIPFRAGF
Sbjct: 316 VETFINGTQWLPQTGIPFRAGF 337

[Close]

BLAST of Chy9G164020 vs. NCBI nr
Match: XP_008465826.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo])

HSP 1 Score: 583 bits (1503), Expect = 1.19e-208
Identity = 283/306 (92.48%), Postives = 298/306 (97.39%), Query Frame = 0


Query: 31  VSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDDSSTTI 90
           +SLDGTGNFVK+NDAIAAAPNFSMTRFYIHVKPGTY+EIIEVP EKT IALIGDDSSTTI
Sbjct: 19  LSLDGTGNFVKVNDAIAAAPNFSMTRFYIHVKPGTYQEIIEVPSEKTFIALIGDDSSTTI 78

Query: 91  IVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 150
           IVNNRSNGTGSSTASSATLTV G NFMAQFLTFQN+AGPNEGQAIA+LDQA HTAYY+CV
Sbjct: 79  IVNNRSNGTGSSTASSATLTVYGENFMAQFLTFQNSAGPNEGQAIAVLDQAKHTAYYRCV 138

Query: 151 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 210
           FLGYQDTLYAG++PQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK S
Sbjct: 139 FLGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKQS 198

Query: 211 LSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 270
           LSA SGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLD+VVQPKGWLEW
Sbjct: 199 LSAPSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWLEW 258

Query: 271 PGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGTQWLPQTGI 330
           PGVPEN+LYYAEYNNSGGGANTSQRVNWPG+HVL+N +EVATF+VE+FINGTQWLPQTGI
Sbjct: 259 PGVPENVLYYAEYNNSGGGANTSQRVNWPGYHVLDNAREVATFSVEMFINGTQWLPQTGI 318

Query: 331 PFRAGF 336
           PFRAGF
Sbjct: 319 PFRAGF 324

[Close]

BLAST of Chy9G164020 vs. NCBI nr
Match: KAA0040551.1 (putative pectinesterase/pectinesterase inhibitor 39 [Cucumis melo var. makuwa] >TYK05594.1 putative pectinesterase/pectinesterase inhibitor 39 [Cucumis melo var. makuwa])

HSP 1 Score: 583 bits (1502), Expect = 1.30e-208
Identity = 283/306 (92.48%), Postives = 298/306 (97.39%), Query Frame = 0


Query: 31  VSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDDSSTTI 90
           VSLDGTGNFVK+NDAIAAAPNFSMTRFYIHVKPGTY+EIIEVP EKT IALIGDDSSTTI
Sbjct: 12  VSLDGTGNFVKVNDAIAAAPNFSMTRFYIHVKPGTYQEIIEVPSEKTFIALIGDDSSTTI 71

Query: 91  IVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 150
           IVNNRSNGTGSSTASSATLTV G NFMA+FLTFQN+AGPNEGQAIA+LDQA HTAYY+CV
Sbjct: 72  IVNNRSNGTGSSTASSATLTVYGENFMARFLTFQNSAGPNEGQAIAVLDQAKHTAYYRCV 131

Query: 151 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKPS 210
           FLGYQDTLYAG++PQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSK S
Sbjct: 132 FLGYQDTLYAGSVPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDVQITVTAQSKQS 191

Query: 211 LSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWLEW 270
           LSA SGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLD+VVQPKGWLEW
Sbjct: 192 LSAPSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDSVVQPKGWLEW 251

Query: 271 PGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGTQWLPQTGI 330
           PGVPEN+LYYAEYNNSGGGANTSQRVNWPG+HVL+N +EVATF+VE+FINGTQWLPQTGI
Sbjct: 252 PGVPENVLYYAEYNNSGGGANTSQRVNWPGYHVLDNAREVATFSVEMFINGTQWLPQTGI 311

Query: 331 PFRAGF 336
           PFRAGF
Sbjct: 312 PFRAGF 317

[Close]

BLAST of Chy9G164020 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 298.1 bits (762), Expect = 8.8e-81
Identity = 147/314 (46.82%), Postives = 200/314 (63.69%), Query Frame = 0


Query: 26  SFNFTVSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDD 85
           ++N +V++DGTGNF  INDA+ AAPN S TRF I++K G Y E +E+P +KT I  IGD 
Sbjct: 219 NYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDG 278

Query: 86  SSTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTA 145
              T+I  NRS   G ST  + T+ V G  ++A+ ++F N+AGP + QA+A    + H+A
Sbjct: 279 IGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSA 338

Query: 146 YYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARLFDV--QITV 205
           +Y+C F GYQDTLY  +  QF++ECDIYG++DFIFGN  VVFQ+ ++YAR  +   +I  
Sbjct: 339 FYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAF 398

Query: 206 TAQSKPSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQ 265
           TAQS+      +G    NC +  +P++   K+    +LGRPWR YS  V I SF+D+++ 
Sbjct: 399 TAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIH 458

Query: 266 PKGWLEW-PGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGT 325
           P GWLE         LYY EY N G GAN ++RV WPGF  + N  E   FTV  FI+G+
Sbjct: 459 PAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGS 518

Query: 326 QWLPQTGIPFRAGF 337
            WL  TGIPF  GF
Sbjct: 519 TWLNSTGIPFSLGF 532

[Close]

BLAST of Chy9G164020 vs. TAIR 10
Match: AT2G26440.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 288.5 bits (737), Expect = 6.9e-78
Identity = 148/313 (47.28%), Postives = 197/313 (62.94%), Query Frame = 0


Query: 26  SFNFTVSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDD 85
           S +  V+ DGTGNF  IN+AI+ APN S  R  I+VK G Y E I++P  KT I LIGD 
Sbjct: 235 SESLVVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDG 294

Query: 86  SSTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTA 145
           S  T I  NRS G G +T  SATL V+G  F+A+ +   NTAGP + QA+AL   A   A
Sbjct: 295 SDVTFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVA 354

Query: 146 YYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYARL-FDVQITV- 205
            Y+CV  GYQDTLY  +  QF++ECDIYG++D+IFGN  VVFQ CNI ++L    Q TV 
Sbjct: 355 LYRCVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVI 414

Query: 206 TAQSKPSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQ 265
           TAQS+ +    +G   QNC++  S ++ +S +KV  +LGRPWR +S  V ++S++D  + 
Sbjct: 415 TAQSRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFID 474

Query: 266 PKGWLEWPGVPE-NLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGT 325
             GW +W G    + LYY EYNN+G G+ T +RVNWPGFH++   ++   FT   FI G 
Sbjct: 475 GSGWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIM-GYEDAFNFTATEFITGD 534

Query: 326 QWLPQTGIPFRAG 336
            WL  T  P+  G
Sbjct: 535 GWLGSTSFPYDNG 546

[Close]

BLAST of Chy9G164020 vs. TAIR 10
Match: AT4G33220.1 (pectin methylesterase 44 )

HSP 1 Score: 285.8 bits (730), Expect = 4.5e-77
Identity = 140/313 (44.73%), Postives = 200/313 (63.90%), Query Frame = 0


Query: 26  SFNFTVSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDD 85
           +++ +V+LDGTGNF KI DAI  AP++S TRF I++K G Y E +E+  +K  I ++GD 
Sbjct: 210 TYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDG 269

Query: 86  SSTTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTA 145
              T+I  NRS   G +T  SAT  V+G  F+A+ +TFQNTAGP + QA+AL   +  + 
Sbjct: 270 IDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSV 329

Query: 146 YYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYAR--LFDVQITV 205
           +++C   GYQDTLY   + QF++EC I G+VDFIFG+G VVFQ+C I A+  L + + T+
Sbjct: 330 FFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNTI 389

Query: 206 TAQSKPSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQ 265
           TAQ +  ++  SGF  Q  N++   ++    +    +LGRPW++YS  VFI + + +VV+
Sbjct: 390 TAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVVR 449

Query: 266 PKGWLEW-PGVPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGT 325
           P+GWLEW      + L+Y E+ N G G+  S RV WPG+HV NN  +   FTV  FI G 
Sbjct: 450 PEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKGN 509

Query: 326 QWLPQTGIPFRAG 336
            WLP TG+ F  G
Sbjct: 510 LWLPSTGVTFSDG 522

[Close]

BLAST of Chy9G164020 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 282.3 bits (721), Expect = 5.0e-76
Identity = 139/308 (45.13%), Postives = 192/308 (62.34%), Query Frame = 0


Query: 31  VSLDGTGNFVKINDAIAAAPNFSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDDSSTTI 90
           V+ DGTGNF  I+DA+ AAP++S  R+ IHVK G Y E +E+  +K  I ++GD    T+
Sbjct: 217 VAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGIDATV 276

Query: 91  IVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTAYYKCV 150
           I  NRS   G +T  SAT  V+G  F+A+ +TFQNTAGP + QA+A+        +Y+C 
Sbjct: 277 ITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCA 336

Query: 151 FLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYAR--LFDVQITVTAQSK 210
             GYQDTLYA ++ QFF+EC I G+VDFIFG+   VFQ C I A+  L + + ++TAQ +
Sbjct: 337 MRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGR 396

Query: 211 PSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQPKGWL 270
              +  +GF  Q  N+    ++  + +    +LGRPW++YS  VF+ +++ + + P GWL
Sbjct: 397 KDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWL 456

Query: 271 EWPG-VPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGTQWLPQ 330
           EW G    + LYY EY NSG GA+  +RV WPG+HVLN   E   FTV   I G  WLP 
Sbjct: 457 EWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPS 516

Query: 331 TGIPFRAG 336
           TGI F AG
Sbjct: 517 TGITFIAG 524

[Close]

BLAST of Chy9G164020 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 278.1 bits (710), Expect = 9.4e-75
Identity = 141/312 (45.19%), Postives = 196/312 (62.82%), Query Frame = 0


Query: 30  TVSLDGTGNFVKINDAIAAAPN---FSMTRFYIHVKPGTYKEIIEVPYEKTCIALIGDDS 89
           TVS DGTGNF  I  A+AAAPN    S   F I+V  G Y+E I +   K  + +IGD  
Sbjct: 266 TVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGI 325

Query: 90  STTIIVNNRSNGTGSSTASSATLTVNGANFMAQFLTFQNTAGPNEGQAIALLDQATHTAY 149
           + T++  NRS   G +T +SAT  V   NF+A  +TF+NTAGP + QA+AL   A  + +
Sbjct: 326 NQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIF 385

Query: 150 YKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVVFQDCNIYAR--LFDVQITVT 209
           Y C F  YQDTLY  +L QF++ECD+YG+VDFIFGN  VVFQ+CN+Y R  + +    +T
Sbjct: 386 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAIT 445

Query: 210 AQSKPSLSALSGFIFQNCNVTVSPEIASSKDKVTVFLGRPWRMYSMVVFIDSFLDNVVQP 269
           AQ +   +  +G   QNC +  + ++ SS   V  +LGRPW+ YS  V++ S++D  V+P
Sbjct: 446 AQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEP 505

Query: 270 KGWLEWPG-VPENLLYYAEYNNSGGGANTSQRVNWPGFHVLNNVKEVATFTVEIFINGTQ 329
            GW EW G    + LYYAEYNN+G G+NT+ RV WPG+HV+N+  + A FTV        
Sbjct: 506 VGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINST-DAANFTVTGLFIEAD 565

Query: 330 WLPQTGIPFRAG 336
           W+ +TG+P+ +G
Sbjct: 566 WIWKTGVPYTSG 576

The following BLAST results are available for this feature:

BLAST of Chy9G164020 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81415	1.2e-79	46.82	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3...	[more]
O04887	6.8e-78	46.77	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O48711	9.8e-77	47.28	Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3...	[more]
Q9SMY7	6.3e-76	44.73	Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3...	[more]
Q43062	1.8e-75	44.30	Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=...	[more]

BLAST of Chy9G164020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1M3	3.1e-174	93.48	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G260430 PE=4 SV=1	[more]
A0A1S3CQ52	4.9e-164	92.48	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503419 PE=4 SV=1	[more]
A0A5D3C2R6	6.4e-164	92.48	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00440 ...	[more]
A0A6J1KPG6	8.7e-145	78.62	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496427 PE=4 SV=1	[more]
A0A6J1GIG0	4.3e-144	77.99	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454459 PE=4 SV=1	[more]

BLAST of Chy9G164020 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654361.2	1.76e-220	92.86	probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus]	[more]
XP_031741746.1	3.73e-220	97.39	probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus] >KAE864851...	[more]
XP_031745525.1	7.67e-215	90.99	probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus]	[more]
XP_008465826.1	1.19e-208	92.48	PREDICTED: probable pectinesterase/pectinesterase inhibitor 39 [Cucumis melo]	[more]
KAA0040551.1	1.30e-208	92.48	putative pectinesterase/pectinesterase inhibitor 39 [Cucumis melo var. makuwa] >...	[more]

BLAST of Chy9G164020 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02300.1	8.8e-81	46.82	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G26440.1	6.9e-78	47.28	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G33220.1	4.5e-77	44.73	pectin methylesterase 44	[more]
AT3G43270.1	5.0e-76	45.13	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G02810.1	9.4e-75	45.19	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 29..321 e-value: 3.1E-102 score: 341.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 25..336 e-value: 4.7E-108 score: 363.3
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 27..334
None	No IPR available	PANTHER	PTHR31707:SF149	PECTINESTERASE/PECTINESTERASE INHIBITOR 17-RELATED	coord: 27..334
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 53..72
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 172..181
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 25..334

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy9G164020.1	Chy9G164020.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity