Chy9G161530 (gene) Cucumber (hystrix) v1

Overview
NameChy9G161530
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionPectinesterase
LocationchrH09: 3888532 .. 3890410 (-)
RNA-Seq ExpressionChy9G161530
SyntenyChy9G161530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCAATAAGGTGGTCTTGCCTGGAATATCCCTTCTTCTTGTTGTTGGTGTAGCACTTGCTGTTGTTGCAATAGTTAACAGCAACAAGAGTTCTGATGCTGCAACAGAGGATTTATCCCCAAAAATGAAGGCAGTTTCAACCATTTGCTCTAATGCTAATTATCAACAGGAATGCCAAAATACACTGAGCAATGCTGCTCATAATGCCTCATCAGATGACCCAAAAGAGTATGTTAAAGCAGCAATTCTTGCCACTATTGAAGAGGTTAAGAAGGGGTATAATTTGACTGATGGATTTATGATTGAGGCTGCTAACAATCGTAGCATTAAAATGGGTGTTGAGGATTGCCGGGACTTGTTGCAATTTGCTATTGATCAGTTGCAAGCATCTTATTCTACCGTTGGTGAACCTGATTTGCATACCAATGCCGATCGTGTCGCAGATATCAAGAATTGGCTAACCTCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAAGAATTTGATCCTCAGCTTAGACAGAAGATGCAAGATGGTCTTGATGATGCCGGCAAACTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGATATTCTTGCAAGCTTTGGCTTGCAATTGAAGGTAATTCTGCTAAATCACTTACTAATTAATCTCCACTACCTTACTTCCATTAAATATATTACAATTTTGTTGGCAGGCTCAACCAAGCGGTCGCAGGCTTCTTGGAACAACAGAGGTAGATAAAGACGGATACCCAACATGGTTGACAGGGGCAGACAGGAAGTTGCTCGCATCAAGAGGAGGAGGCGCTAAAGTGAAACCAAACGCAGTGGTGGCTAAGGACGGTAGCGGACAGTTCAAAACCATTTCTGCTGCATTGGCTGCATATCCAAAGGCCTTAAGAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAAGGAATACGTCCATATCACTAAAGACATGAAAAACATTTTCATGTATGGAGATGGCCCAAAGAAAACCATCGTCACCGGTAACAAGAGCAACCGTGGTGGCTTCACCACCCAAGATACTGCCACTTTCAGTAAGTGAATAAATTTACCACATGACAAGTATGATCGGACAATTTGATGAAATCACTCACTATTTTTCTTTCAACTTACACGTATATTTATACTTGTAGTTGCCATTGGAGAAGGATTCCTTTGCAAATCGATGGGATTCCAAAACACAGCCGGTCCTGAAGGCCACCAAGCAGTAGCTCTTAGAGTCCAGTCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACTCAACGTCAATTCTACCGTAACTGTGTCATATCAGGCACTGTCGATTTCATCTTTGGCGATTCAACCACCGTCATCCAAAACTCCTTGATCATCGTTCGTCGCCCCATGGACAACCAACAGAACACCGTCACAGCCAATGGTCGCGCAGATCCCAAGGAAGTCTCAGGCTTGGTGATCCACAACTGCAGAATCGTCCCAGAACAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGGAATATGCAAGAACTGTGATCATGGAGTCTACTTTGGGTGACTTCATTCAGCCAGTTGGATACATGCCTTGGTCTGGAAATTTTGCATTGGAGACATGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTGTTACCAACAGGAGAGTGAGATGGAAGGGTGTTAAAGTGATCGGAAGAAATGAAGCCATGCAATTCACTGCTGGATCTTTTCTTCAAGGAAGAGCTTGGCTTCCAACCACTGGTGGCCCATATCTATTGGGATTGAAAGGCTAA

mRNA sequence

ATGGTCAATAAGGTGGTCTTGCCTGGAATATCCCTTCTTCTTGTTGTTGGTGTAGCACTTGCTGTTGTTGCAATAGTTAACAGCAACAAGAGTTCTGATGCTGCAACAGAGGATTTATCCCCAAAAATGAAGGCAGTTTCAACCATTTGCTCTAATGCTAATTATCAACAGGAATGCCAAAATACACTGAGCAATGCTGCTCATAATGCCTCATCAGATGACCCAAAAGAGTATGTTAAAGCAGCAATTCTTGCCACTATTGAAGAGGTTAAGAAGGGGTATAATTTGACTGATGGATTTATGATTGAGGCTGCTAACAATCGTAGCATTAAAATGGGTGTTGAGGATTGCCGGGACTTGTTGCAATTTGCTATTGATCAGTTGCAAGCATCTTATTCTACCGTTGGTGAACCTGATTTGCATACCAATGCCGATCGTGTCGCAGATATCAAGAATTGGCTAACCTCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAAGAATTTGATCCTCAGCTTAGACAGAAGATGCAAGATGGTCTTGATGATGCCGGCAAACTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGATATTCTTGCAAGCTTTGGCTTGCAATTGAAGGCTCAACCAAGCGGTCGCAGGCTTCTTGGAACAACAGAGGTAGATAAAGACGGATACCCAACATGGTTGACAGGGGCAGACAGGAAGTTGCTCGCATCAAGAGGAGGAGGCGCTAAAGTGAAACCAAACGCAGTGGTGGCTAAGGACGGTAGCGGACAGTTCAAAACCATTTCTGCTGCATTGGCTGCATATCCAAAGGCCTTAAGAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAAGGAATACGTCCATATCACTAAAGACATGAAAAACATTTTCATGTATGGAGATGGCCCAAAGAAAACCATCGTCACCGGTAACAAGAGCAACCGTGGTGGCTTCACCACCCAAGATACTGCCACTTTCATTGCCATTGGAGAAGGATTCCTTTGCAAATCGATGGGATTCCAAAACACAGCCGGTCCTGAAGGCCACCAAGCAGTAGCTCTTAGAGTCCAGTCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACTCAACGTCAATTCTACCGTAACTGTGTCATATCAGGCACTGTCGATTTCATCTTTGGCGATTCAACCACCGTCATCCAAAACTCCTTGATCATCGTTCGTCGCCCCATGGACAACCAACAGAACACCGTCACAGCCAATGGTCGCGCAGATCCCAAGGAAGTCTCAGGCTTGGTGATCCACAACTGCAGAATCGTCCCAGAACAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGGAATATGCAAGAACTGTGATCATGGAGTCTACTTTGGGTGACTTCATTCAGCCAGTTGGATACATGCCTTGGTCTGGAAATTTTGCATTGGAGACATGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTGTTACCAACAGGAGAGTGAGATGGAAGGGTGTTAAAGTGATCGGAAGAAATGAAGCCATGCAATTCACTGCTGGATCTTTTCTTCAAGGAAGAGCTTGGCTTCCAACCACTGGTGGCCCATATCTATTGGGATTGAAAGGCTAA

Coding sequence (CDS)

ATGGTCAATAAGGTGGTCTTGCCTGGAATATCCCTTCTTCTTGTTGTTGGTGTAGCACTTGCTGTTGTTGCAATAGTTAACAGCAACAAGAGTTCTGATGCTGCAACAGAGGATTTATCCCCAAAAATGAAGGCAGTTTCAACCATTTGCTCTAATGCTAATTATCAACAGGAATGCCAAAATACACTGAGCAATGCTGCTCATAATGCCTCATCAGATGACCCAAAAGAGTATGTTAAAGCAGCAATTCTTGCCACTATTGAAGAGGTTAAGAAGGGGTATAATTTGACTGATGGATTTATGATTGAGGCTGCTAACAATCGTAGCATTAAAATGGGTGTTGAGGATTGCCGGGACTTGTTGCAATTTGCTATTGATCAGTTGCAAGCATCTTATTCTACCGTTGGTGAACCTGATTTGCATACCAATGCCGATCGTGTCGCAGATATCAAGAATTGGCTAACCTCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAAGAATTTGATCCTCAGCTTAGACAGAAGATGCAAGATGGTCTTGATGATGCCGGCAAACTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGATATTCTTGCAAGCTTTGGCTTGCAATTGAAGGCTCAACCAAGCGGTCGCAGGCTTCTTGGAACAACAGAGGTAGATAAAGACGGATACCCAACATGGTTGACAGGGGCAGACAGGAAGTTGCTCGCATCAAGAGGAGGAGGCGCTAAAGTGAAACCAAACGCAGTGGTGGCTAAGGACGGTAGCGGACAGTTCAAAACCATTTCTGCTGCATTGGCTGCATATCCAAAGGCCTTAAGAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATAAGGAATACGTCCATATCACTAAAGACATGAAAAACATTTTCATGTATGGAGATGGCCCAAAGAAAACCATCGTCACCGGTAACAAGAGCAACCGTGGTGGCTTCACCACCCAAGATACTGCCACTTTCATTGCCATTGGAGAAGGATTCCTTTGCAAATCGATGGGATTCCAAAACACAGCCGGTCCTGAAGGCCACCAAGCAGTAGCTCTTAGAGTCCAGTCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACTCAACGTCAATTCTACCGTAACTGTGTCATATCAGGCACTGTCGATTTCATCTTTGGCGATTCAACCACCGTCATCCAAAACTCCTTGATCATCGTTCGTCGCCCCATGGACAACCAACAGAACACCGTCACAGCCAATGGTCGCGCAGATCCCAAGGAAGTCTCAGGCTTGGTGATCCACAACTGCAGAATCGTCCCAGAACAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGGAATATGCAAGAACTGTGATCATGGAGTCTACTTTGGGTGACTTCATTCAGCCAGTTGGATACATGCCTTGGTCTGGAAATTTTGCATTGGAGACATGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTGTTACCAACAGGAGAGTGAGATGGAAGGGTGTTAAAGTGATCGGAAGAAATGAAGCCATGCAATTCACTGCTGGATCTTTTCTTCAAGGAAGAGCTTGGCTTCCAACCACTGGTGGCCCATATCTATTGGGATTGAAAGGCTAA

Protein sequence

MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQNTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQDGLDDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSFLQGRAWLPTTGGPYLLGLKG*
Homology
BLAST of Chy9G161530 vs. ExPASy Swiss-Prot
Match: O80722 (Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.7e-127
Identity = 266/606 (43.89%), Postives = 365/606 (60.23%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQ 60
           M+ KVV+   S+LL+VGVA+ VVA +  NK+ DA   +LSP+MKAV  IC + + +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFI--NKNGDA---NLSPQMKAVQGICQSTSDKASCV 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIE-----AANNRSIKMGVE 120
            TL        S+DP + +KA +LAT +E+ K  N T    +      + NN+++   ++
Sbjct: 61  KTL----EPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAV---LD 120

Query: 121 DCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQL 180
            C+ +  +A++ L      +GE DL     ++  +K WL  V +YQ  CLD +EE D  L
Sbjct: 121 YCKRVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--L 180

Query: 181 RQKMQDGLDDAGKLTSNALAIVDAVSDILASFGLQLK----------------------- 240
           R+ + +G+ ++  LT+NA+ I   V   +A    ++                        
Sbjct: 181 RKAIGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPV 240

Query: 241 AQPSG--RRLLGTTEVDKDGYPTWLTGADRKLLA--------SRGGGAKVKPNAVVAKDG 300
           A P G  RRLL   ++D+ G PTW++GADRKL+A         RGGGA+V+ N VVAKDG
Sbjct: 241 ADPDGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDG 300

Query: 301 SGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNK 360
           SGQFKT+  A+ A P+  RGR +IY+KAG+Y+E V I K   NIFM+GDG +KT+++ N+
Sbjct: 301 SGQFKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNR 360

Query: 361 S--NRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDG 420
           S     G TT  +AT     EGF+ K MGF+NTAGP GHQA A+RV  DR+  FNCR DG
Sbjct: 361 SVALSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDG 420

Query: 421 YQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADP 480
           YQDTLYV   RQFYRNCV+SGTVDFIFG S TVIQN+LI+VR+    Q NTVTA+G    
Sbjct: 421 YQDTLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELG 480

Query: 481 KEVS-GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQPVGYMPW 540
             +  G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + +GD I+P G+  W
Sbjct: 481 LGMKIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIW 540

Query: 541 SGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSFLQGRAWLPTTGG 566
            G    ++C Y+EY NRGPGA  NRRV W  V      E   FTA ++L    W+     
Sbjct: 541 DGESFHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANV 588

BLAST of Chy9G161530 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 7.8e-127
Identity = 227/562 (40.39%), Postives = 351/562 (62.46%), Query Frame = 0

Query: 4   KVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQNTL 63
           + V+  IS +L++ + +AV   V+ NKS +A  E+++  +KA+  +C+  +Y++ C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  SNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDLLQF 123
              A + S  DP E VK A  AT++++      +   MIE   +   KM ++ C++L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQ-TMIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQDGL 183
           AI +L  S+  +G+ + H   + +  ++ WL++ IS++Q+CLDG +       + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 DDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGADRKLL 243
             A +LT N LA+V  +S+ L    +    + + RRLL         +P+W+    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQI---PEMNSRRLL------SQEFPSWMDARARRLL 254

Query: 244 ASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHITKDM 303
                 ++VKP+ VVA+DGSGQ+KTI+ AL   PK     +V+++K GIYKEYV + + M
Sbjct: 255 --NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 314

Query: 304 KNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVA 363
            ++   GDGP KT+++G+KS + G TT  TAT   +G+ F+ K++ F+NTAG   HQAVA
Sbjct: 315 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 374

Query: 364 LRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRR 423
           +RV +D S F+NC+ DGYQDTLY  + RQFYR+C ISGT+DF+FGD+  V QN  ++VR+
Sbjct: 375 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 434

Query: 424 PMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIM 483
           P+ NQ   +TA+GR DP+E +G V+  C IV E    A + +  T+LGRPWKEY+RT+IM
Sbjct: 435 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 494

Query: 484 ESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFT 543
            + + DF+ P G+ PW G F L T  Y E  N GPGA   +RV W G+K +   E ++FT
Sbjct: 495 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 554

Query: 544 AGSFLQGRAWLPTTGGPYLLGL 566
              ++QG AW+P  G PY+LGL
Sbjct: 555 PAQYIQGDAWIPGKGVPYILGL 562

BLAST of Chy9G161530 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 449.1 bits (1154), Expect = 7.3e-125
Identity = 223/558 (39.96%), Postives = 343/558 (61.47%), Query Frame = 0

Query: 10  ISLLLVVGVALAVVAIVNSNK--SSDAATEDLSPKMKAVSTICSNANYQQECQNTLSNAA 69
           IS +L++ + +AV   V+ NK         +++  +KAV  +C+  +Y++ C++TL    
Sbjct: 20  ISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLIKNG 79

Query: 70  HNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDLLQFAIDQ 129
            N +  DP E VK A   T++++      +   M E   +   +M ++ C++L+ +A+D+
Sbjct: 80  KNTT--DPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDYALDE 139

Query: 130 LQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQDGLDDAG 189
           L  S+  +G+ + H   + + +++ WL++ IS++++CL+G +       + M+  L  A 
Sbjct: 140 LSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALKTAI 199

Query: 190 KLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGADRKLLASRG 249
           +LT N LAI+  +S+ +    +Q+    S RRLL       +G+P+W+    RKLL +  
Sbjct: 200 ELTHNGLAIISEMSNFVGQ--MQIPGLNS-RRLLA------EGFPSWVDQRGRKLLQAAA 259

Query: 250 GGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHITKDMKNIF 309
             + VKP+ VVA+DGSGQ+KTI+ AL   PK     +V+++KAG+YKEYV + K M ++ 
Sbjct: 260 AYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLV 319

Query: 310 MYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQ 369
             GDGP KTI++GNK+ + G TT  TAT   +G  F+ K++GF+NTAG   HQAVA+RVQ
Sbjct: 320 FIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQ 379

Query: 370 SDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDN 429
           SD S FFNCR DGYQDTLY  + RQF+R+C ISGT+DF+FGD+  V QN  ++VR+P+ N
Sbjct: 380 SDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPN 439

Query: 430 QQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTL 489
           Q   +TA+GR DP+E +G V   C I  E    A +     +LGRPWKEY+RT+IM + +
Sbjct: 440 QACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFI 499

Query: 490 GDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSF 549
            DF+QP G+ PW G+F L+T  Y E  N GPG+    RV W G+K +   + ++FT   +
Sbjct: 500 PDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQY 559

Query: 550 LQGRAWLPTTGGPYLLGL 566
           +QG  W+P  G PY  GL
Sbjct: 560 IQGDDWIPGKGVPYTTGL 565

BLAST of Chy9G161530 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 2.1e-124
Identity = 251/598 (41.97%), Postives = 362/598 (60.54%), Query Frame = 0

Query: 4   KVVLPGISLLLV----VGVALAVVAIVN---------SNKSSDAATEDLSPKM------- 63
           +++L  +S+L+V    +G A A V   N         +NKS D+ T+  SP         
Sbjct: 24  RIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSAA 83

Query: 64  ---------KAVSTICSNANYQQECQNTLSN-AAHNASSDDPKEYVKAAILATIEEVKKG 123
                    K + T+C++  Y+  CQNTL N    +    DP+  +K+AI+A  +++ + 
Sbjct: 84  QTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQV 143

Query: 124 YNLTDGFMIEAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNW 183
           +        E   N+  K  +  C+ L+  A ++L  S   + + +++  A  V D+ +W
Sbjct: 144 FKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSW 203

Query: 184 LTSVISYQQSCLDGLEEFDPQLRQKMQDGLDDAGKLTSNALAIVDAVSDILASF-----G 243
           L++V+SYQ++C+DG EE   +L+ +++   + +  LTSN+LA++ ++   L+S       
Sbjct: 204 LSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKTR 263

Query: 244 LQLKAQPSGRRLLGTTEVDKDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKT 303
           L L+A+ S +        + D   +WL+  +R++L +    A +KPNA VAKDGSG F T
Sbjct: 264 LLLEARSSAK--------ETDHITSWLSNKERRMLKAVDVKA-LKPNATVAKDGSGNFTT 323

Query: 304 ISAALAAYPKALRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGF 363
           I+AAL A P   +GRY IY+K GIY E V I K   N+ M GDG +KTIVTGNKS+    
Sbjct: 324 INAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKI 383

Query: 364 TTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQ 423
            T  TATF+A GEGF+ +SMGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  
Sbjct: 384 RTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAY 443

Query: 424 TQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVI 483
           T RQ+YR+CVI GTVDFIFGD+  + QN  I +R+ +  Q+NTVTA GR D  + +G VI
Sbjct: 444 THRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVI 503

Query: 484 HNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQPVGYMPW-SGNFALET 543
           HNC + P + L   + +  ++LGRPWK ++RTV+MEST+ D I PVG++ W   +FA++T
Sbjct: 504 HNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDT 563

Query: 544 CLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSFLQGRAWLPTTGGPYLLGL 566
             Y EY N GP   T  RV+W G +V+ + EAM+FT G FLQG  W+   G P  LGL
Sbjct: 564 LSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of Chy9G161530 vs. ExPASy Swiss-Prot
Match: Q5MFV8 (Pectinesterase 5 OS=Arabidopsis thaliana OX=3702 GN=PME5 PE=1 SV=2)

HSP 1 Score: 447.6 bits (1150), Expect = 2.1e-124
Identity = 265/614 (43.16%), Postives = 368/614 (59.93%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQ 60
           M+ KVV+   S+LL+VGVA+ VVA +  NK+ DA   +LSP+MKAV  IC   + +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAYI--NKNGDA---NLSPQMKAVRGICEATSDKASCV 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNL---TDGFMIE--AANNRSIKMGVE 120
            TL        SDDP + +KA +LAT + + +  N    T+G +    + NN+++   ++
Sbjct: 61  KTL----EPVKSDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAV---LD 120

Query: 121 DCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQL 180
            C+ +  +A++ L      +GE DL+    ++  +K WLT V +YQ  CLD +EE D  L
Sbjct: 121 YCKKVFMYALEDLSTIVEEMGE-DLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEEDD--L 180

Query: 181 RQKMQDGLDDAGKLTSNALAIVDAVSDILASFGLQLK----------------------- 240
           R+ + +G+  +  LTSNA+ I   V   +A   L+++                       
Sbjct: 181 RKTIGEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKG 240

Query: 241 ----------AQPSG--RRLLGTTEVDKDGYPTWLTGADRKLLASRG-----GGAKVKPN 300
                     A P G  RRLL   ++D+ G PTW++GADRKL+   G     GGA+++  
Sbjct: 241 TPPVADDSPVADPDGPARRLL--EDIDETGIPTWVSGADRKLMTKAGRGSNDGGARIRAT 300

Query: 301 AVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKK 360
            VVAKDGSGQFKT+  A+ A P+   GR +I++KAGIY+E V I K   NIFM+GDG +K
Sbjct: 301 FVVAKDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARK 360

Query: 361 TIVTGNKSNR--GGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAF 420
           T+++ N+S +   G TT  + T     EGF+ K +GF+NTAGP GHQAVA+RV  DR+  
Sbjct: 361 TVISYNRSVKLSPGTTTSLSGTVQVESEGFMAKWIGFKNTAGPMGHQAVAIRVNGDRAVI 420

Query: 421 FNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVT 480
           FNCR DGYQDTLYV   RQFYRN V+SGTVDFIFG S TVIQNSLI+VR+    Q NTVT
Sbjct: 421 FNCRFDGYQDTLYVNNGRQFYRNIVVSGTVDFIFGKSATVIQNSLIVVRKGNKGQFNTVT 480

Query: 481 ANGRADPKEVS-GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQ 540
           A+G      +  G+V+ NCRIVP++KL AER  + ++LGRPWK+++ TVI+ S +GD I+
Sbjct: 481 ADGNEKGLAMKIGIVLQNCRIVPDKKLAAERLIVESYLGRPWKKFSTTVIINSEIGDVIR 540

Query: 541 PVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVI-GRNEAMQFTAGSFLQGR 566
           P G+  W G    ++C Y+EY NRGPGA+TNRRV W  VK+     E   FT  ++L   
Sbjct: 541 PEGWKIWDGESFHKSCRYVEYNNRGPGAITNRRVNW--VKIARSAAEVNDFTVANWLGPI 595

BLAST of Chy9G161530 vs. ExPASy TrEMBL
Match: A0A5A7U9J8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100 PE=3 SV=1)

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 553/566 (97.70%), Postives = 562/566 (99.29%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQ 60
           MVNKV+LPGISL+LVVGVALAVVAIV+SNKSSDAATEDLSPKMKAVSTIC+NANYQQECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYV+AAIL+TIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180
           LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQL+QKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGADR 240
           DGL DAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEV KDGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHIT 300
           KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIY EYVHIT
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTT+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGSFLQGRAWLPTTGGPYLLGLK 567
           QFTAG FLQGRAWLPTTGGPYLLGLK
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLK 566

BLAST of Chy9G161530 vs. ExPASy TrEMBL
Match: A0A1S3CR27 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 552/566 (97.53%), Postives = 562/566 (99.29%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQ 60
           MVNKV+LPGI+L+LVVGVALAVVAIV+SNKSSDAATEDLSPKMKAVSTIC+NANYQQECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYV+AAIL+TIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180
           LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQL+QKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGADR 240
           DGL DAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEV KDGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHIT 300
           KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIY EYVHIT
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTT+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGSFLQGRAWLPTTGGPYLLGLK 567
           QFTAG FLQGRAWLPTTGGPYLLGLK
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLK 566

BLAST of Chy9G161530 vs. ExPASy TrEMBL
Match: A0A5A7U7W4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090 PE=3 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 1.1e-277
Identity = 478/566 (84.45%), Postives = 512/566 (90.46%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQ 60
           MV KVV+  ++ LLVVGV LAV A+VN N SSD  T D+SPKMKAVS+ICSN +YQQECQ
Sbjct: 1   MVAKVVM-AVTSLLVVGVVLAVFAMVNKNHSSD-ETADMSPKMKAVSSICSNTDYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
            TL N  HN+S DDPKE++KAAI ATIEE+KKGYNLTD  M+EAANN SIKM V+DCRDL
Sbjct: 61  TTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180
           LQ AID+L ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQL+QKMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGADR 240
            GLD A KLTSNALAIVDAVSDIL S GLQLK QPSGRRLLGTTEVD DGYPTWLTGADR
Sbjct: 181 GGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHIT 300
           KLLA++ GG +VKPNAVVAKDGSGQ+KTI+AALAAYPKAL+GRYVIYVKAGIY EY+ +T
Sbjct: 241 KLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKN+FMYGDGP+KTIVTG KSNR G  TQ+TATF AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCV+SGTVDFIFGDS T+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIME+TLGDFIQP GYMPWSG+FAL TC Y EYGNRGPGA T RRVRWKGV+VIGRNEAM
Sbjct: 481 VIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAM 540

Query: 541 QFTAGSFLQGRAWLPTTGGPYLLGLK 567
           QFTAG FL GRAWLP TGGPYLLGLK
Sbjct: 541 QFTAGPFLLGRAWLPATGGPYLLGLK 564

BLAST of Chy9G161530 vs. ExPASy TrEMBL
Match: A0A1S3CR40 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503798 PE=3 SV=1)

HSP 1 Score: 961.8 bits (2485), Expect = 1.2e-276
Identity = 472/557 (84.74%), Postives = 506/557 (90.84%), Query Frame = 0

Query: 10  ISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQNTLSNAAHN 69
           ++ LLVVGV LAV A+VN N SSD  T D+SPKMKAVS+ICSN +YQQECQ TL N  HN
Sbjct: 3   VTSLLVVGVVLAVFAMVNKNHSSD-ETADMSPKMKAVSSICSNTDYQQECQTTLGNVGHN 62

Query: 70  ASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDLLQFAIDQLQ 129
           +S DDPKE++KAAI ATIEE+KKGYNLTD  M+EAANN SIKM V+DCRDLLQ AID+L 
Sbjct: 63  SSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDELH 122

Query: 130 ASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQDGLDDAGKL 189
           ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQL+QKMQ GLD A KL
Sbjct: 123 ASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAKL 182

Query: 190 TSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGADRKLLASRGGG 249
           TSNALAIVDAVSDIL S GLQLK QPSGRRLLGTTEVD DG+PTWLTGADRKLLA++ GG
Sbjct: 183 TSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADRKLLAAKRGG 242

Query: 250 AKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHITKDMKNIFMY 309
            +VKPNAVVAKDGSGQ+KTI+AALAAYPKAL+GRYVIYVKAGIY EY+ +TKDMKN+FMY
Sbjct: 243 VRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFMY 302

Query: 310 GDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSD 369
           GDGP+KTIVTG KSNR G  TQ+TATF AIGEGFLCKSMGF NTAGPEGHQAVALRVQSD
Sbjct: 303 GDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSD 362

Query: 370 RSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQ 429
           RSAFFNCRMDGYQDTLYVQT RQFYRNCV+SGTVDFIFGDS T+IQNSLIIVRRPMDNQQ
Sbjct: 363 RSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQQ 422

Query: 430 NTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGD 489
           NTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIME+TLGD
Sbjct: 423 NTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLGD 482

Query: 490 FIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSFLQ 549
           FIQP GYMPWSG+FAL TC Y EYGNRGPGA T RRVRWKGV+VIGRNEAMQFTAG FL 
Sbjct: 483 FIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAGPFLL 542

Query: 550 GRAWLPTTGGPYLLGLK 567
           GRAWLP TGGPYLLGLK
Sbjct: 543 GRAWLPATGGPYLLGLK 558

BLAST of Chy9G161530 vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 961.4 bits (2484), Expect = 1.6e-276
Identity = 473/566 (83.57%), Postives = 514/566 (90.81%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQ 60
           MV KVV+ GISL+LVVGVALAVVAIVN + SSD  T +LSPKMKAV++ICSNA+YQ ECQ
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSD-ETANLSPKMKAVASICSNADYQPECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
            TL +  +N+S +DPKE++KAAI +TIEE+KKGYNLTD  M+EAANN +IKM V+DC+DL
Sbjct: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180
           LQ AID+L ASYSTVG+PDLHTN DR+ADIKNWLT+VISYQQSCLDGLEEFDPQL+QKMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGADR 240
           DGLD AGKLTSNALAIV AVSDIL   GLQLK QPSGRRLLGTTEVD D +PTWLTG+DR
Sbjct: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHIT 300
           KLLA++ GG +VKPN VVAKDGSGQ+KTI AALAAYPKAL+GRYVIYVKAG+Y E + +T
Sbjct: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKNIFMYGDGP+KTIVTG KSNR G TTQ+TA+F AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS T+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIMESTLGDFIQP GYMPWSGNFAL TC Y EYGNRGPGA TNRRVRWKGV+VIGRNEAM
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540

Query: 541 QFTAGSFLQGRAWLPTTGGPYLLGLK 567
           QFTAG FL G+AWLP TGGPYLLGLK
Sbjct: 541 QFTAGPFLLGKAWLPGTGGPYLLGLK 565

BLAST of Chy9G161530 vs. NCBI nr
Match: KAA0050275.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98132.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1103 bits (2853), Expect = 0.0
Identity = 553/566 (97.70%), Postives = 562/566 (99.29%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQ 60
           MVNKV+LPGISL+LVVGVALAVVAIV+SNKSSDAATEDLSPKMKAVSTIC+NANYQQECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYV+AAIL+TIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180
           LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQL+QKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGADR 240
           DGL DAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEV KDGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHIT 300
           KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIY EYVHIT
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTT+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGSFLQGRAWLPTTGGPYLLGLK 566
           QFTAG FLQGRAWLPTTGGPYLLGLK
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLK 566

BLAST of Chy9G161530 vs. NCBI nr
Match: XP_008466374.1 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1102 bits (2850), Expect = 0.0
Identity = 552/566 (97.53%), Postives = 562/566 (99.29%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQ 60
           MVNKV+LPGI+L+LVVGVALAVVAIV+SNKSSDAATEDLSPKMKAVSTIC+NANYQQECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYV+AAIL+TIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180
           LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQL+QKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGADR 240
           DGL DAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEV KDGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHIT 300
           KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIY EYVHIT
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTT+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGSFLQGRAWLPTTGGPYLLGLK 566
           QFTAG FLQGRAWLPTTGGPYLLGLK
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLK 566

BLAST of Chy9G161530 vs. NCBI nr
Match: XP_038875878.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 1002 bits (2591), Expect = 0.0
Identity = 501/567 (88.36%), Postives = 533/567 (94.00%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQ 60
           MVNKVVLPGISL+LVVGVALAVVA+VN N +S   TE+LSPKMKAVSTICSN NYQ+ECQ
Sbjct: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNNST--TENLSPKMKAVSTICSNTNYQEECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
           NTL+N AHNASSDDPKEYVKAAILAT+EEVKKGYNLTDGFMIEAANN SIKMGVEDC+DL
Sbjct: 61  NTLTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180
           LQFAI QLQASY+TVGEPDLHTNADRVADIKNWLT+VISYQQSCLDGL EFDP L+QKMQ
Sbjct: 121 LQFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQ 180

Query: 181 DGLDDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGADR 240
           DGLD A KLTSNALAIVD VSDIL +FGLQ K QPSGR+LLGT EV  DG+PTW++GADR
Sbjct: 181 DGLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHIT 300
           KLLASRGGG +VKPNAVVAKDGSGQFKTI AALAAYPKAL+GRYVIYVKAGIY EYV +T
Sbjct: 241 KLLASRGGG-RVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDM NIFMYGDGPK+TIVTG +SNR G+TTQDTATFIAIG GFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420
           AVALRVQSD+SAFFNCRMDGYQDTLYVQT+RQFYRNCVISGTVDFIFGDSTT+IQNSLII
Sbjct: 361 AVALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPT+LGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIME+TLGDFI+P GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGVKVI R EA+
Sbjct: 481 VIMETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEAL 540

Query: 541 QFTAGSFLQGRAWLPTTGGPYLLGLKG 567
           +FTAGSFLQG +WLP TGGPYLLGLKG
Sbjct: 541 KFTAGSFLQGGSWLPGTGGPYLLGLKG 564

BLAST of Chy9G161530 vs. NCBI nr
Match: XP_038875879.1 (LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida])

HSP 1 Score: 959 bits (2480), Expect = 0.0
Identity = 477/567 (84.13%), Postives = 519/567 (91.53%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLS-PKMKAVSTICSNANYQQEC 60
           MV KVV+ GISL+LVVGVALAVVA+V  N +SD    DLS PKMKAVSTICS  +YQQEC
Sbjct: 1   MVGKVVVSGISLILVVGVALAVVAMVTKNNNSDV---DLSSPKMKAVSTICSTTDYQQEC 60

Query: 61  QNTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRD 120
           QNTL+N A NAS DDPKEY KAA+LATI+E+ KGYNLT   M+EAANN SIKM V+DC+D
Sbjct: 61  QNTLTNVAQNASVDDPKEYAKAAVLATIQEITKGYNLTADLMVEAANNASIKMSVDDCKD 120

Query: 121 LLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKM 180
           LLQ AID+L ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQL+QKM
Sbjct: 121 LLQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKM 180

Query: 181 QDGLDDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGAD 240
           QDGLD AGKLTSNALAIV AVSDIL +FGLQLK QPSGRRLL TTE+  DG+PTW++GAD
Sbjct: 181 QDGLDVAGKLTSNALAIVGAVSDILKNFGLQLKVQPSGRRLLETTEIGSDGFPTWMSGAD 240

Query: 241 RKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHI 300
           RKLLASRGGGA+VKPNAVVAKDGSGQ+K+I AALAAYPKALRGRYVIYVKAGIY EY+ +
Sbjct: 241 RKLLASRGGGARVKPNAVVAKDGSGQYKSIGAALAAYPKALRGRYVIYVKAGIYDEYITV 300

Query: 301 TKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGH 360
           TKDMKN+FMYGDGP+KTIVTG K+NR G TTQ+TA+F AIGEGFLCKSMGF+NTAGPEGH
Sbjct: 301 TKDMKNVFMYGDGPRKTIVTGRKNNRDGITTQNTASFSAIGEGFLCKSMGFRNTAGPEGH 360

Query: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLI 420
           QAVALRVQSDRSAF+NCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS+T+IQNSLI
Sbjct: 361 QAVALRVQSDRSAFYNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYAR 480
           IVRRPMDNQ+NTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YAR
Sbjct: 421 IVRRPMDNQKNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEA 540
           TVIME+TLGDFIQP GYMPWSGNFALETC Y EYGNRGPGA TNRRVRWKGV+VIGRNEA
Sbjct: 481 TVIMETTLGDFIQPDGYMPWSGNFALETCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEA 540

Query: 541 MQFTAGSFLQGRAWLPTTGGPYLLGLK 566
           +Q+TAG FL GRAWLP TGGPYLLGLK
Sbjct: 541 LQYTAGPFLMGRAWLPATGGPYLLGLK 564

BLAST of Chy9G161530 vs. NCBI nr
Match: KAA0050276.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98131.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 957 bits (2473), Expect = 0.0
Identity = 478/566 (84.45%), Postives = 512/566 (90.46%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQ 60
           MV KVV+  ++ LLVVGV LAV A+VN N SSD  T D+SPKMKAVS+ICSN +YQQECQ
Sbjct: 1   MVAKVVM-AVTSLLVVGVVLAVFAMVNKNHSSDE-TADMSPKMKAVSSICSNTDYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
            TL N  HN+S DDPKE++KAAI ATIEE+KKGYNLTD  M+EAANN SIKM V+DCRDL
Sbjct: 61  TTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180
           LQ AID+L ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQL+QKMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGADR 240
            GLD A KLTSNALAIVDAVSDIL S GLQLK QPSGRRLLGTTEVD DGYPTWLTGADR
Sbjct: 181 GGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHIT 300
           KLLA++ GG +VKPNAVVAKDGSGQ+KTI+AALAAYPKAL+GRYVIYVKAGIY EY+ +T
Sbjct: 241 KLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKN+FMYGDGP+KTIVTG KSNR G  TQ+TATF AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCV+SGTVDFIFGDS T+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIME+TLGDFIQP GYMPWSG+FAL TC Y EYGNRGPGA T RRVRWKGV+VIGRNEAM
Sbjct: 481 VIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAM 540

Query: 541 QFTAGSFLQGRAWLPTTGGPYLLGLK 566
           QFTAG FL GRAWLP TGGPYLLGLK
Sbjct: 541 QFTAGPFLLGRAWLPATGGPYLLGLK 564

BLAST of Chy9G161530 vs. TAIR 10
Match: AT2G47030.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 457.2 bits (1175), Expect = 1.9e-128
Identity = 266/606 (43.89%), Postives = 365/606 (60.23%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQ 60
           M+ KVV+   S+LL+VGVA+ VVA +  NK+ DA   +LSP+MKAV  IC + + +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFI--NKNGDA---NLSPQMKAVQGICQSTSDKASCV 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIE-----AANNRSIKMGVE 120
            TL        S+DP + +KA +LAT +E+ K  N T    +      + NN+++   ++
Sbjct: 61  KTL----EPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAV---LD 120

Query: 121 DCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQL 180
            C+ +  +A++ L      +GE DL     ++  +K WL  V +YQ  CLD +EE D  L
Sbjct: 121 YCKRVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--L 180

Query: 181 RQKMQDGLDDAGKLTSNALAIVDAVSDILASFGLQLK----------------------- 240
           R+ + +G+ ++  LT+NA+ I   V   +A    ++                        
Sbjct: 181 RKAIGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPV 240

Query: 241 AQPSG--RRLLGTTEVDKDGYPTWLTGADRKLLA--------SRGGGAKVKPNAVVAKDG 300
           A P G  RRLL   ++D+ G PTW++GADRKL+A         RGGGA+V+ N VVAKDG
Sbjct: 241 ADPDGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDG 300

Query: 301 SGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNK 360
           SGQFKT+  A+ A P+  RGR +IY+KAG+Y+E V I K   NIFM+GDG +KT+++ N+
Sbjct: 301 SGQFKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNR 360

Query: 361 S--NRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDG 420
           S     G TT  +AT     EGF+ K MGF+NTAGP GHQA A+RV  DR+  FNCR DG
Sbjct: 361 SVALSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDG 420

Query: 421 YQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADP 480
           YQDTLYV   RQFYRNCV+SGTVDFIFG S TVIQN+LI+VR+    Q NTVTA+G    
Sbjct: 421 YQDTLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELG 480

Query: 481 KEVS-GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQPVGYMPW 540
             +  G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + +GD I+P G+  W
Sbjct: 481 LGMKIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIW 540

Query: 541 SGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSFLQGRAWLPTTGG 566
            G    ++C Y+EY NRGPGA  NRRV W  V      E   FTA ++L    W+     
Sbjct: 541 DGESFHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANV 588

BLAST of Chy9G161530 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 455.7 bits (1171), Expect = 5.5e-128
Identity = 227/562 (40.39%), Postives = 351/562 (62.46%), Query Frame = 0

Query: 4   KVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQNTL 63
           + V+  IS +L++ + +AV   V+ NKS +A  E+++  +KA+  +C+  +Y++ C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  SNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDLLQF 123
              A + S  DP E VK A  AT++++      +   MIE   +   KM ++ C++L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQ-TMIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQDGL 183
           AI +L  S+  +G+ + H   + +  ++ WL++ IS++Q+CLDG +       + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 DDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGADRKLL 243
             A +LT N LA+V  +S+ L    +    + + RRLL         +P+W+    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQI---PEMNSRRLL------SQEFPSWMDARARRLL 254

Query: 244 ASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHITKDM 303
                 ++VKP+ VVA+DGSGQ+KTI+ AL   PK     +V+++K GIYKEYV + + M
Sbjct: 255 --NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 314

Query: 304 KNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVA 363
            ++   GDGP KT+++G+KS + G TT  TAT   +G+ F+ K++ F+NTAG   HQAVA
Sbjct: 315 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 374

Query: 364 LRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRR 423
           +RV +D S F+NC+ DGYQDTLY  + RQFYR+C ISGT+DF+FGD+  V QN  ++VR+
Sbjct: 375 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 434

Query: 424 PMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIM 483
           P+ NQ   +TA+GR DP+E +G V+  C IV E    A + +  T+LGRPWKEY+RT+IM
Sbjct: 435 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 494

Query: 484 ESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFT 543
            + + DF+ P G+ PW G F L T  Y E  N GPGA   +RV W G+K +   E ++FT
Sbjct: 495 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 554

Query: 544 AGSFLQGRAWLPTTGGPYLLGL 566
              ++QG AW+P  G PY+LGL
Sbjct: 555 PAQYIQGDAWIPGKGVPYILGL 562

BLAST of Chy9G161530 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 449.1 bits (1154), Expect = 5.2e-126
Identity = 223/558 (39.96%), Postives = 343/558 (61.47%), Query Frame = 0

Query: 10  ISLLLVVGVALAVVAIVNSNK--SSDAATEDLSPKMKAVSTICSNANYQQECQNTLSNAA 69
           IS +L++ + +AV   V+ NK         +++  +KAV  +C+  +Y++ C++TL    
Sbjct: 20  ISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLIKNG 79

Query: 70  HNASSDDPKEYVKAAILATIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDLLQFAIDQ 129
            N +  DP E VK A   T++++      +   M E   +   +M ++ C++L+ +A+D+
Sbjct: 80  KNTT--DPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDYALDE 139

Query: 130 LQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQDGLDDAG 189
           L  S+  +G+ + H   + + +++ WL++ IS++++CL+G +       + M+  L  A 
Sbjct: 140 LSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALKTAI 199

Query: 190 KLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDKDGYPTWLTGADRKLLASRG 249
           +LT N LAI+  +S+ +    +Q+    S RRLL       +G+P+W+    RKLL +  
Sbjct: 200 ELTHNGLAIISEMSNFVGQ--MQIPGLNS-RRLLA------EGFPSWVDQRGRKLLQAAA 259

Query: 250 GGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHITKDMKNIF 309
             + VKP+ VVA+DGSGQ+KTI+ AL   PK     +V+++KAG+YKEYV + K M ++ 
Sbjct: 260 AYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLV 319

Query: 310 MYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQ 369
             GDGP KTI++GNK+ + G TT  TAT   +G  F+ K++GF+NTAG   HQAVA+RVQ
Sbjct: 320 FIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQ 379

Query: 370 SDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDN 429
           SD S FFNCR DGYQDTLY  + RQF+R+C ISGT+DF+FGD+  V QN  ++VR+P+ N
Sbjct: 380 SDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPN 439

Query: 430 QQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTL 489
           Q   +TA+GR DP+E +G V   C I  E    A +     +LGRPWKEY+RT+IM + +
Sbjct: 440 QACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFI 499

Query: 490 GDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSF 549
            DF+QP G+ PW G+F L+T  Y E  N GPG+    RV W G+K +   + ++FT   +
Sbjct: 500 PDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQY 559

Query: 550 LQGRAWLPTTGGPYLLGL 566
           +QG  W+P  G PY  GL
Sbjct: 560 IQGDDWIPGKGVPYTTGL 565

BLAST of Chy9G161530 vs. TAIR 10
Match: AT2G47040.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 447.6 bits (1150), Expect = 1.5e-125
Identity = 265/614 (43.16%), Postives = 368/614 (59.93%), Query Frame = 0

Query: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDAATEDLSPKMKAVSTICSNANYQQECQ 60
           M+ KVV+   S+LL+VGVA+ VVA +  NK+ DA   +LSP+MKAV  IC   + +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAYI--NKNGDA---NLSPQMKAVRGICEATSDKASCV 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNL---TDGFMIE--AANNRSIKMGVE 120
            TL        SDDP + +KA +LAT + + +  N    T+G +    + NN+++   ++
Sbjct: 61  KTL----EPVKSDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAV---LD 120

Query: 121 DCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQL 180
            C+ +  +A++ L      +GE DL+    ++  +K WLT V +YQ  CLD +EE D  L
Sbjct: 121 YCKKVFMYALEDLSTIVEEMGE-DLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEEDD--L 180

Query: 181 RQKMQDGLDDAGKLTSNALAIVDAVSDILASFGLQLK----------------------- 240
           R+ + +G+  +  LTSNA+ I   V   +A   L+++                       
Sbjct: 181 RKTIGEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKG 240

Query: 241 ----------AQPSG--RRLLGTTEVDKDGYPTWLTGADRKLLASRG-----GGAKVKPN 300
                     A P G  RRLL   ++D+ G PTW++GADRKL+   G     GGA+++  
Sbjct: 241 TPPVADDSPVADPDGPARRLL--EDIDETGIPTWVSGADRKLMTKAGRGSNDGGARIRAT 300

Query: 301 AVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKK 360
            VVAKDGSGQFKT+  A+ A P+   GR +I++KAGIY+E V I K   NIFM+GDG +K
Sbjct: 301 FVVAKDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARK 360

Query: 361 TIVTGNKSNR--GGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAF 420
           T+++ N+S +   G TT  + T     EGF+ K +GF+NTAGP GHQAVA+RV  DR+  
Sbjct: 361 TVISYNRSVKLSPGTTTSLSGTVQVESEGFMAKWIGFKNTAGPMGHQAVAIRVNGDRAVI 420

Query: 421 FNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVT 480
           FNCR DGYQDTLYV   RQFYRN V+SGTVDFIFG S TVIQNSLI+VR+    Q NTVT
Sbjct: 421 FNCRFDGYQDTLYVNNGRQFYRNIVVSGTVDFIFGKSATVIQNSLIVVRKGNKGQFNTVT 480

Query: 481 ANGRADPKEVS-GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQ 540
           A+G      +  G+V+ NCRIVP++KL AER  + ++LGRPWK+++ TVI+ S +GD I+
Sbjct: 481 ADGNEKGLAMKIGIVLQNCRIVPDKKLAAERLIVESYLGRPWKKFSTTVIINSEIGDVIR 540

Query: 541 PVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVI-GRNEAMQFTAGSFLQGR 566
           P G+  W G    ++C Y+EY NRGPGA+TNRRV W  VK+     E   FT  ++L   
Sbjct: 541 PEGWKIWDGESFHKSCRYVEYNNRGPGAITNRRVNW--VKIARSAAEVNDFTVANWLGPI 595

BLAST of Chy9G161530 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 447.6 bits (1150), Expect = 1.5e-125
Identity = 251/598 (41.97%), Postives = 362/598 (60.54%), Query Frame = 0

Query: 4   KVVLPGISLLLV----VGVALAVVAIVN---------SNKSSDAATEDLSPKM------- 63
           +++L  +S+L+V    +G A A V   N         +NKS D+ T+  SP         
Sbjct: 24  RIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSAA 83

Query: 64  ---------KAVSTICSNANYQQECQNTLSN-AAHNASSDDPKEYVKAAILATIEEVKKG 123
                    K + T+C++  Y+  CQNTL N    +    DP+  +K+AI+A  +++ + 
Sbjct: 84  QTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQV 143

Query: 124 YNLTDGFMIEAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNW 183
           +        E   N+  K  +  C+ L+  A ++L  S   + + +++  A  V D+ +W
Sbjct: 144 FKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSW 203

Query: 184 LTSVISYQQSCLDGLEEFDPQLRQKMQDGLDDAGKLTSNALAIVDAVSDILASF-----G 243
           L++V+SYQ++C+DG EE   +L+ +++   + +  LTSN+LA++ ++   L+S       
Sbjct: 204 LSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKTR 263

Query: 244 LQLKAQPSGRRLLGTTEVDKDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKT 303
           L L+A+ S +        + D   +WL+  +R++L +    A +KPNA VAKDGSG F T
Sbjct: 264 LLLEARSSAK--------ETDHITSWLSNKERRMLKAVDVKA-LKPNATVAKDGSGNFTT 323

Query: 304 ISAALAAYPKALRGRYVIYVKAGIYKEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGF 363
           I+AAL A P   +GRY IY+K GIY E V I K   N+ M GDG +KTIVTGNKS+    
Sbjct: 324 INAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKI 383

Query: 364 TTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQ 423
            T  TATF+A GEGF+ +SMGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  
Sbjct: 384 RTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAY 443

Query: 424 TQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVI 483
           T RQ+YR+CVI GTVDFIFGD+  + QN  I +R+ +  Q+NTVTA GR D  + +G VI
Sbjct: 444 THRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVI 503

Query: 484 HNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQPVGYMPW-SGNFALET 543
           HNC + P + L   + +  ++LGRPWK ++RTV+MEST+ D I PVG++ W   +FA++T
Sbjct: 504 HNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDT 563

Query: 544 CLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGSFLQGRAWLPTTGGPYLLGL 566
             Y EY N GP   T  RV+W G +V+ + EAM+FT G FLQG  W+   G P  LGL
Sbjct: 564 LSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O807222.7e-12743.89Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1[more]
Q3E8Z87.8e-12740.39Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q8GX867.3e-12539.96Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q9SMY62.1e-12441.97Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3... [more]
Q5MFV82.1e-12443.16Pectinesterase 5 OS=Arabidopsis thaliana OX=3702 GN=PME5 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7U9J80.0e+0097.70Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100... [more]
A0A1S3CR270.0e+0097.53Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1[more]
A0A5A7U7W41.1e-27784.45Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090... [more]
A0A1S3CR401.2e-27684.74Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503798 PE=3 SV=1[more]
A0A0A0KLN21.6e-27683.57Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAA0050275.10.097.70pectinesterase-like [Cucumis melo var. makuwa] >TYJ98132.1 pectinesterase-like [... [more]
XP_008466374.10.097.53PREDICTED: pectinesterase-like [Cucumis melo][more]
XP_038875878.10.088.36pectinesterase-like [Benincasa hispida][more]
XP_038875879.10.084.13LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida][more]
KAA0050276.10.084.45pectinesterase-like [Cucumis melo var. makuwa] >TYJ98131.1 pectinesterase-like [... [more]
Match NameE-valueIdentityDescription
AT2G47030.11.9e-12843.89Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.15.5e-12840.39Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.15.2e-12639.96Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47040.11.5e-12543.16Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33230.11.5e-12541.97Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 41..196
e-value: 1.2E-32
score: 124.5
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 20..198
e-value: 1.5E-17
score: 61.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 45..196
e-value: 7.3E-24
score: 84.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 43..201
e-value: 3.0E-29
score: 103.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 44..200
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 255..550
e-value: 9.0E-131
score: 435.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 249..565
e-value: 3.0E-111
score: 373.8
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 4..565
NoneNo IPR availablePANTHERPTHR31707:SF164PECTINESTERASE 4-RELATEDcoord: 4..565
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 48..201
e-value: 1.09298E-46
score: 158.756
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 399..408
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 252..564

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy9G161530.1Chy9G161530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity