Chy9G161010 (gene) Cucumber (hystrix) v1

Overview
NameChy9G161010
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionPectate lyase
LocationchrH09: 3383806 .. 3388856 (+)
RNA-Seq ExpressionChy9G161010
SyntenyChy9G161010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCGTCAATTCGTTCTCCTCGCCCTTCTCTTCTCCTCCGCCGTCGTCGGCATTGTCTCTGCCGCCGAAGCCCCCGCCGCCTCCCCCGCCACCTCCCCTGCTTCCTCCCCTAAGGCCGCTGACGTTGAACCCTCCGATGGCGGAAAAACTCCTGCAGCCGCTCCTTCCAAGTCTCCCGCCGCAGCCGCTGGCGCCCCCATCGCTGCCACTCCTTCCGCTGCCGGTGCTCCCAAAGGAGCTGCTTCTGCCCCTTCAGCCAAGGCTGGAGCCCCTGAAGCTGACACCCCTGTTGCCGGTGGCCCTGCTTCTTCCCCTGCTGGCGATGAGTCTTCTTCCACTGTGGCCCCTGCTGGAGCACCTGAAGCCGATGCCGATGTGTCATCTCCACCTTCCCCAACTGGACCTGCTGCCGGACCTGCAAGCGATGCCGTTGCTGATGCACCAGCTGATTCACCAGCTGATACACCCTCAGATTCTCCGGCCAATGGTGGTTCTTTGGTGAAAGTGTCTGTTGCGGCCGTTGCTGGGATTTCGGGCATTGTTGGATTGTTTGTGTTCTAAGATTGTAATTTTTCTAAATTTAATGAGATTAATGAGATGGGTTTGAATTCTATTCGAAGAGAGATCAATGGTTTCTGTTTTTTTTTTTATTATTATATGTATTTGATGATTCTCTGAGAATACAAAAGAGGATGATCATATGATCATTTTTTATCTTTTTAAAATTCTCTTCACTCTTTCAACCTTCTTTCTCTTTTCTGGATTACTCCTTAAAACAATTTCTTTTTTCAAACTCATTCAAATCATTCAAATCTATATCCACTAAATTGTCTAATTTAAAGAAAACACAGATTGTTAATAAGATTTAAAGAATTTCTTAGTATGTTTGTTTTGATACATCATTTCTTTTTTAAAAAAATTAATTTATAACTTAGTACTTTATATTATATGATTTTTCTAATTGAATATAATATCTTACATAAGAGCTAAACGAGCTCCTTAATTTCTAGCATTTAAGTTTATTATTCTTACTATTGAAAACCATAACACAAGTCTCACCATCTTCTCTCTAAATTTCTCTCTTCAAAAATTTACTTTCATTCACAAATATTTAAATATTATATCGTAAAATTATATTTATGCAAACCTTAAACAAAATTGATTTGTACTTCTTTTTTTCTTTTCTTTTTTTTTTTTGTAGAGGAGAAATGTTTGAAAAAACAGGCAATTGAGAGAGTATCTTTCTACAACAAACTTAGACGTAATTTGTGGGATTTTGATAGTCAACAACCTCTATCATTCTCTTTTCGAACATTGGATCCAAAATTAATTCATTTTACTTGTCTCAAATTTGTAGAAAAACTTTAATTGTGTTCATAATTTCAATTTTAAATTATGAAACGAGATCTCAAACGACGTCCTACACTAATGTGTTAGGCTGCTAATAAAAAAAAATGCATTTGTTTCTTTTTCATACCTGTTAGAGTATTTAGAATTAAATTGTAATACTTTAATATTATGTTGTTATGTTAATGTTATGTAGTTAAGGTTAATTTCTGTTGTATTTAACGTTTTTTTTAAACGTTGTTCTACAGTGTGTGAAACCCTAATGGAAGATTTGTCAGCTCTCTCTACTTCTCTTTTATCTCTCTAACTCTGTAGTAGTTTAATTTTCAATGTACTTTTTTTGAAGGTCTTTATCTATAATCTTTCTACTTTTGTTTGTAACCGAATAGTGCAAACCAATCATAAAATAAACATGTTATATTCAATCACAAAATTTCATTATAATAATTTGTGGTGCTATACAATAAATATATCTAGAGTTGGTCGATGTAATGTCCATGAAAAGTACTTCAAAGCAAAAGTAGTTAATATACGATTGTATTACTTTAACTCTCAACTGACAGTTTGTATGTACATTCAATACAATAAAATAATACTTACAAGTATATCATGAGCCTACTGATATACTTTATTGACCTAATTAATATCTCAATTATATATTAATTCCAGAAAGGTAATATTTGGATGTTCATATTTATTAGTTCTTAGAGGGGTTAATTAAAATCCTCACACTATATACTTTAGGAGTCAAAAGCAGTTGCAATAATTATACTGGTCCTTTGGTGTGAATCTAAAATCAGAAATTCTCCCATTGATGGCTCTAAATCCATCAAAAGTCGCCAAGGAAAAAAAAAGTATGACCTAATTAGTTTTATCTAAAGTGTTTTAAGAAACACATAGAAGGTCTCAAACTTTAATTTGAAAGACTCCGTGAGTTTTGATAAAATATTAGAATTAAGTGTGTTATTTTATATCGAAAAGATTCACTAAAGCGAGGAGTCCATGACTTTGGTTTCAAATTGTTTTTATAAATACATTTGAAGCTTAATTGCGCTAACTCTAACTAGTTAATTTTTTTTTTTAATCATCTCATTTGAAATTGTGTGGAGGAAAAAGTTATGAGTGGAACAAATGCATTTATGTTCGTATTGTTTTAGGATTAGGAAAAGATTATTATTTATTTGTGATGGTATTTTCTAGTTTGAGTGGTCGTTCAAAAGCTTTTTATAGAGTGTGCTTAATAATTGAGATAATGAAAAGATTTATTCCATGTGATTGTAACAATTTCACACCTTATAAGGATTTATTTCTATAGTGAGTTGAAATTTGAAGATTTTCACATAAATTCTTGTGTTTCTTTTTTTGTTACTTACTTTAAGTCTCTATACTTAGAATCTCACATCCAACAATAATTCTTGCGTTTCTCACTTTGGTATAGAAAATATATACAGTAAAAATGTTCATACGTTAAAATCTACATCCAACAGTTATTATGAGATCGTTCCCACCATAATAGCACAAAATAACAATGTGAAACTATCAACTATATTGCGACGATGATTCGAGAGGAAGACATGAGAGAAAAAGGAAACAAAAAGGAAAAAGTCAAAGTGAATTGACTTAAGTGTGGTTTGAAGATTGAAGAGAGAGGGTTCAAAAAACCTGAAATTACGATAATGTTAGAGACCCCACAGATGTAATTTATGGTACAATCACGCCTCTACTATCGGCTTTATATTTCTATATAAATCACATATATCAATCCCCATTTTCCCTAAACTCTAAAACCAATTTTTCAATCTTTCTTTTTTCTTTTGTTTAATTTTGAACTCTCTAATGGCGGCTTCTTCTTCTTCCTCTCTTTACTTTTTGTTGCTTCTTTTATCTCTTTCTTTCTTCTTGTCTATGACTGCTCCAATACCTTCCAATTCCTCCTTACCTTTTGTTCATCAACGTCCTCATCTCGTCGTTGAAGAAGTTCAAAGGTATATACATTTTTGTTATGTACATTGGTTCTCATACATATTACATCTACTCTTGTTTTAGTTCTTGTACTTTTAAATCATTTAATTTTTCGGTCATGCATGCATTTTTATAACACAAATCTTTTATGTAACAAAGTTTTAGGGTTAAACTTCACTCACTTGTGAAAATAAATTTATATGGGAAGAGTAGTTGTTATGATGAGTAAATTTTAATTTGTGTTATTATTTTGTAGATCCAATTAAAATAAACTCTTTTTAAAAGAGTAAAACATGACAAGTAGACACCTTAAAAGGTGGGAATGTAATGTGACAAAGGAACAAACGTTGAAAGTTTACTATTTTAAAGAATGAGAACCTAACATATAGAACCAAACCAAAACTGTATTCAAAATTTTGGGGAAAATACTTTTTAGTGTATCTATGTTTTGATATACATTTGGTAATTGAATTAGTTATTATTTTTGGTTGTAAAATAAATTTGTGGTTACTGATGGCGGCACGCAGGAGCATAAATGGATCAAGAAGAAACTTGGGGTATTTATCATGTGGAACAGGAAACCCAATAGACGATTGTTGGAGATGCGATTCCAATTGGGAAACGAACCGTAAACGCTTAGCCGATTGCGCCATCGGCTTCGGCAAAAACGCAATCGGCGGCAAAAACGGCCGTTTCTACGTAGTCACCGACTCCGGGAACGACGACCCACTGAACCCCCGTCCGGGCACTCTCCGCCACGCCGTCATCCAAAACGAACCCTTATGGATCATCTTCAAGCGCGACATGGTAATCAAACTCGCCCAAGAACTGGTCATGAACTCCTTCAAAACCATCGACGGACGTGGCGCCAGCGTCCACTTGGCAGGTGGCCCTTGTATTAAAATCCACTACGCCACAAATATAATCATCCACGGCATCCATATTCACGATTGCAAGAGAGGTGGGAATGCCAACATACGAGACTCCCCACAGCATGCTGGCTGGTGGACTGCATCTGACGGTGATGGTGTCTCAATTTTAGGTGGTAAACAAATCTGGATCGATCATTGCTCTTTGTCTAATTGTAATGATGGGTTGATTGACGCCATTCATGGATCAACGGCTATTACTATATCCAATAACTATATGACACATCATGATAAGGTTATGTTATTGGGACATAGTGATTCTTTCACCCAAGATAAAAATATGCAAGTTACTATTGCTTTTAACCATTTTGGTGAAGGACTTGTCCAACGAATGCCAAGGTAAATTTAACTTTAACTACTACAAAATTTCATATCCCTTTTCATCATCTCTACAATATTACTTTCATTCTCAATTAAAAAAGATTGAAACCTATATGCTATATGTGTAGATGTAGACATGGATATTTCCATGTAGTGAACAACGATTACACTCATTGGGAAATGTATGCCATCGGAGGGAGTGCTTCGCCCACGATATACAGCCAAGGCAATAGATTCGTGGCACCTTATAATCGATTTCGAAAGGAGGTGACAAAGCACGAGGACGCACCCGAGAGCGAGTGGCGCCATTGGAACTGGCGTTCTGAGGGTGACTTGTTGTTGAATGGCGCCTATTTTCGCCAGTCAGGCGCGGGTGCCTCCTCTAGCTATGCACGAGCTTATAGTCTCAGTGCTAGACCCTCGTCTCTTGTTGGGGATATCACTATTACTGCGGGTGCTCTCAATTGTCGCAGAGGTTCTCGTTGCTAA

mRNA sequence

ATGGCCCGTCAATTCGTTCTCCTCGCCCTTCTCTTCTCCTCCGCCGTCGTCGGCATTGTCTCTGCCGCCGAAGCCCCCGCCGCCTCCCCCGCCACCTCCCCTGCTTCCTCCCCTAAGGCCGCTGACGTTGAACCCTCCGATGGCGGAAAAACTCCTGCAGCCGCTCCTTCCAAGTCTCCCGCCGCAGCCGCTGGCGCCCCCATCGCTGCCACTCCTTCCGCTGCCGGTGCTCCCAAAGGAGCTGCTTCTGCCCCTTCAGCCAAGGCTGGAGCCCCTGAAGCTGACACCCCTGTTGCCGGTGGCCCTGCTTCTTCCCCTGCTGGCGATGAGTCTTCTTCCACTGTGGCCCCTGCTGGAGCACCTGAAGCCGATGCCGATGTGTCATCTCCACCTTCCCCAACTGGACCTGCTGCCGGACCTGCAAGCGATGCCGTTGCTGATGCACCAGCTGATTCACCAGCTGATACACCCTCAGATTCTCCGGCCAATGGTGGTTCTTTGGTGAAAGTGTCTGTTGCGGCCGTTGCTGGGATTTCGGGCATTGTTGGATTGAGCATAAATGGATCAAGAAGAAACTTGGGGTATTTATCATGTGGAACAGGAAACCCAATAGACGATTGTTGGAGATGCGATTCCAATTGGGAAACGAACCGTAAACGCTTAGCCGATTGCGCCATCGGCTTCGGCAAAAACGCAATCGGCGGCAAAAACGGCCGTTTCTACGTAGTCACCGACTCCGGGAACGACGACCCACTGAACCCCCGTCCGGGCACTCTCCGCCACGCCGTCATCCAAAACGAACCCTTATGGATCATCTTCAAGCGCGACATGGTAATCAAACTCGCCCAAGAACTGGTCATGAACTCCTTCAAAACCATCGACGGACGTGGCGCCAGCGTCCACTTGGCAGGTGGCCCTTGTATTAAAATCCACTACGCCACAAATATAATCATCCACGGCATCCATATTCACGATTGCAAGAGAGGTGGGAATGCCAACATACGAGACTCCCCACAGCATGCTGGCTGGTGGACTGCATCTGACGGTGATGGTGTCTCAATTTTAGGTGGTAAACAAATCTGGATCGATCATTGCTCTTTGTCTAATTGTAATGATGGGTTGATTGACGCCATTCATGGATCAACGGCTATTACTATATCCAATAACTATATGACACATCATGATAAGGTTATGTTATTGGGACATAGTGATTCTTTCACCCAAGATAAAAATATGCAAGTTACTATTGCTTTTAACCATTTTGGTGAAGGACTTGTCCAACGAATGCCAAGATGTAGACATGGATATTTCCATGTAGTGAACAACGATTACACTCATTGGGAAATGTATGCCATCGGAGGGAGTGCTTCGCCCACGATATACAGCCAAGGCAATAGATTCGTGGCACCTTATAATCGATTTCGAAAGGAGGTGACAAAGCACGAGGACGCACCCGAGAGCGAGTGGCGCCATTGGAACTGGCGTTCTGAGGGTGACTTGTTGTTGAATGGCGCCTATTTTCGCCAGTCAGGCGCGGGTGCCTCCTCTAGCTATGCACGAGCTTATAGTCTCAGTGCTAGACCCTCGTCTCTTGTTGGGGATATCACTATTACTGCGGGTGCTCTCAATTGTCGCAGAGGTTCTCGTTGCTAA

Coding sequence (CDS)

ATGGCCCGTCAATTCGTTCTCCTCGCCCTTCTCTTCTCCTCCGCCGTCGTCGGCATTGTCTCTGCCGCCGAAGCCCCCGCCGCCTCCCCCGCCACCTCCCCTGCTTCCTCCCCTAAGGCCGCTGACGTTGAACCCTCCGATGGCGGAAAAACTCCTGCAGCCGCTCCTTCCAAGTCTCCCGCCGCAGCCGCTGGCGCCCCCATCGCTGCCACTCCTTCCGCTGCCGGTGCTCCCAAAGGAGCTGCTTCTGCCCCTTCAGCCAAGGCTGGAGCCCCTGAAGCTGACACCCCTGTTGCCGGTGGCCCTGCTTCTTCCCCTGCTGGCGATGAGTCTTCTTCCACTGTGGCCCCTGCTGGAGCACCTGAAGCCGATGCCGATGTGTCATCTCCACCTTCCCCAACTGGACCTGCTGCCGGACCTGCAAGCGATGCCGTTGCTGATGCACCAGCTGATTCACCAGCTGATACACCCTCAGATTCTCCGGCCAATGGTGGTTCTTTGGTGAAAGTGTCTGTTGCGGCCGTTGCTGGGATTTCGGGCATTGTTGGATTGAGCATAAATGGATCAAGAAGAAACTTGGGGTATTTATCATGTGGAACAGGAAACCCAATAGACGATTGTTGGAGATGCGATTCCAATTGGGAAACGAACCGTAAACGCTTAGCCGATTGCGCCATCGGCTTCGGCAAAAACGCAATCGGCGGCAAAAACGGCCGTTTCTACGTAGTCACCGACTCCGGGAACGACGACCCACTGAACCCCCGTCCGGGCACTCTCCGCCACGCCGTCATCCAAAACGAACCCTTATGGATCATCTTCAAGCGCGACATGGTAATCAAACTCGCCCAAGAACTGGTCATGAACTCCTTCAAAACCATCGACGGACGTGGCGCCAGCGTCCACTTGGCAGGTGGCCCTTGTATTAAAATCCACTACGCCACAAATATAATCATCCACGGCATCCATATTCACGATTGCAAGAGAGGTGGGAATGCCAACATACGAGACTCCCCACAGCATGCTGGCTGGTGGACTGCATCTGACGGTGATGGTGTCTCAATTTTAGGTGGTAAACAAATCTGGATCGATCATTGCTCTTTGTCTAATTGTAATGATGGGTTGATTGACGCCATTCATGGATCAACGGCTATTACTATATCCAATAACTATATGACACATCATGATAAGGTTATGTTATTGGGACATAGTGATTCTTTCACCCAAGATAAAAATATGCAAGTTACTATTGCTTTTAACCATTTTGGTGAAGGACTTGTCCAACGAATGCCAAGATGTAGACATGGATATTTCCATGTAGTGAACAACGATTACACTCATTGGGAAATGTATGCCATCGGAGGGAGTGCTTCGCCCACGATATACAGCCAAGGCAATAGATTCGTGGCACCTTATAATCGATTTCGAAAGGAGGTGACAAAGCACGAGGACGCACCCGAGAGCGAGTGGCGCCATTGGAACTGGCGTTCTGAGGGTGACTTGTTGTTGAATGGCGCCTATTTTCGCCAGTCAGGCGCGGGTGCCTCCTCTAGCTATGCACGAGCTTATAGTCTCAGTGCTAGACCCTCGTCTCTTGTTGGGGATATCACTATTACTGCGGGTGCTCTCAATTGTCGCAGAGGTTCTCGTTGCTAA

Protein sequence

MARQFVLLALLFSSAVVGIVSAAEAPAASPATSPASSPKAADVEPSDGGKTPAAAPSKSPAAAAGAPIAATPSAAGAPKGAASAPSAKAGAPEADTPVAGGPASSPAGDESSSTVAPAGAPEADADVSSPPSPTGPAAGPASDAVADAPADSPADTPSDSPANGGSLVKVSVAAVAGISGIVGLSINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVTDSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDAPESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNCRRGSRC*
Homology
BLAST of Chy9G161010 vs. ExPASy Swiss-Prot
Match: O24554 (Pectate lyase OS=Zinnia violacea OX=34245 PE=1 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 1.4e-189
Identity = 305/366 (83.33%), Postives = 333/366 (90.98%), Query Frame = 0

Query: 185 SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT 244
           SIN SRRNLGYLSCGTGNPIDDCWRCD NW  NR+RLADCAIGFGKNA+GG+NGR YVVT
Sbjct: 36  SINASRRNLGYLSCGTGNPIDDCWRCDPNWANNRQRLADCAIGFGKNAMGGRNGRIYVVT 95

Query: 245 DSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAG 304
           D GNDDP+NP PGTLR+AVIQ+EPLWIIFKRDMVI+L QELVMNS KTIDGRG +VH+  
Sbjct: 96  DPGNDDPVNPVPGTLRYAVIQDEPLWIIFKRDMVIQLRQELVMNSHKTIDGRGVNVHIGN 155

Query: 305 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH 364
           GPCI IHYA+NIIIHGIHIHDCK+ GN NIR+SP H+GWWT SDGDG+SI   K IWIDH
Sbjct: 156 GPCITIHYASNIIIHGIHIHDCKQAGNGNIRNSPHHSGWWTQSDGDGISIFASKDIWIDH 215

Query: 365 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG 424
            SLSNC+DGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDS+TQDKNMQVTIAFNHFGEG
Sbjct: 216 NSLSNCHDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEG 275

Query: 425 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA 484
           LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRF+AP  RF KEVTKHE+A
Sbjct: 276 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFLAPNTRFDKEVTKHENA 335

Query: 485 PESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNC 544
           PESEW++WNWRSEGDL+LNGAYFR+SG  A+SS+ARA SLS RPS+LV  +T +AGAL C
Sbjct: 336 PESEWKNWNWRSEGDLMLNGAYFRESGGRAASSFARASSLSGRPSTLVASMTRSAGALVC 395

Query: 545 RRGSRC 551
           R+GSRC
Sbjct: 396 RKGSRC 401

BLAST of Chy9G161010 vs. ExPASy Swiss-Prot
Match: Q9LTZ0 (Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2)

HSP 1 Score: 658.3 bits (1697), Expect = 7.7e-188
Identity = 301/364 (82.69%), Postives = 326/364 (89.56%), Query Frame = 0

Query: 187 NGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVTDS 246
           N SRR+L YLSC TGNPIDDCWRCD NWETNR+RLADCAIGFGKNAIGG+ GR YVVTD 
Sbjct: 49  NASRRSLAYLSCRTGNPIDDCWRCDPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDP 108

Query: 247 GNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAGGP 306
            NDDP+NPRPGTLR+AV Q EPLWIIFKRDMVI+L +EL++ SFKTIDGRG+SVH+  GP
Sbjct: 109 ANDDPVNPRPGTLRYAVTQEEPLWIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGP 168

Query: 307 CIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDHCS 366
           C+KIHYATNIIIHGI+IHDCK G    I+D P H GWW  SDGD V+I GGK +WIDHCS
Sbjct: 169 CLKIHYATNIIIHGINIHDCKPGSGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCS 228

Query: 367 LSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEGLV 426
           LSNC+DGLIDAIHGSTAITISNN+MTHHDKVMLLGHSDS+TQDKNMQVTIAFNHFGEGLV
Sbjct: 229 LSNCDDGLIDAIHGSTAITISNNHMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLV 288

Query: 427 QRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDAPE 486
           QRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRF+AP  RF KEVTKHEDAPE
Sbjct: 289 QRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPE 348

Query: 487 SEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNCRR 546
           S+WR WNWRSEGD+LLNGAYFR+SGA A S+YARA SLSARPSSLVG IT TAG L+CRR
Sbjct: 349 SKWRDWNWRSEGDMLLNGAYFRESGAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRR 408

Query: 547 GSRC 551
           G RC
Sbjct: 409 GRRC 412

BLAST of Chy9G161010 vs. ExPASy Swiss-Prot
Match: Q9C5M8 (Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2)

HSP 1 Score: 639.8 bits (1649), Expect = 2.8e-182
Identity = 293/369 (79.40%), Postives = 330/369 (89.43%), Query Frame = 0

Query: 185 SINGS---RRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFY 244
           SIN S   RR LGYLSC TGNPIDDCWRCD +WE +R+RLADCAIGFGKNAIGG++GR Y
Sbjct: 40  SINASVAGRRKLGYLSCTTGNPIDDCWRCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIY 99

Query: 245 VVTDSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVH 304
           VVTDSGND+P++P+PGTLRHAV+Q+EPLWIIF+RDM I+L +EL+MNSFKTIDGRGASVH
Sbjct: 100 VVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKEELIMNSFKTIDGRGASVH 159

Query: 305 LAGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIW 364
           ++GGPCI I Y TNIIIHGIHIHDCK+GGNA +R SP+H GW T SDGDGVSI GG  +W
Sbjct: 160 ISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVW 219

Query: 365 IDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHF 424
           +DHCS SNC DGLIDAI GSTAIT+SNN+MTHHDKVMLLGHSD++++DKNMQVTIAFNHF
Sbjct: 220 VDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHF 279

Query: 425 GEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKH 484
           GEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTI SQGNRF+AP  RF KEVTKH
Sbjct: 280 GEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPNIRFSKEVTKH 339

Query: 485 EDAPESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGA 544
           EDAPESEW+ WNWRS GDLLLNGA+F  SG  ASSSYA+A SL A+PSSLVG +T T+GA
Sbjct: 340 EDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASSLGAKPSSLVGPLTSTSGA 399

Query: 545 LNCRRGSRC 551
           LNCR+GSRC
Sbjct: 400 LNCRKGSRC 408

BLAST of Chy9G161010 vs. ExPASy Swiss-Prot
Match: P24396 (Probable pectate lyase P18 OS=Solanum lycopersicum OX=4081 GN=9612 PE=2 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 9.1e-181
Identity = 298/370 (80.54%), Postives = 326/370 (88.11%), Query Frame = 0

Query: 185 SINGS--RRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYV 244
           SIN S  RRNLGYLSCG+GNPID                + CAIGFGKNAIGGKNGR YV
Sbjct: 35  SINASLTRRNLGYLSCGSGNPIDRLLAMQPQLGKKSPAFSYCAIGFGKNAIGGKNGRIYV 94

Query: 245 VTDSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHL 304
           VTDSGNDDP+NP+PGTLRHAVIQ+EPLWIIFKRDMVI+L QELVMNS+KTIDGRGASVH+
Sbjct: 95  VTDSGNDDPVNPKPGTLRHAVIQDEPLWIIFKRDMVIQLKQELVMNSYKTIDGRGASVHI 154

Query: 305 AGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWI 364
           +GGPCI IH+ +NIIIHGI+IHDCK+ GN NIRDSP H+GWW  SDGDG+SI GGK IW+
Sbjct: 155 SGGPCITIHHTSNIIIHGINIHDCKQSGNGNIRDSPNHSGWWDVSDGDGISIFGGKNIWV 214

Query: 365 DHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFG 424
           DHCSLSNC+DGLIDAIHGSTAITISNNY THHDKVMLLGHSDSFTQDK MQVT+AFNHFG
Sbjct: 215 DHCSLSNCHDGLIDAIHGSTAITISNNYFTHHDKVMLLGHSDSFTQDKGMQVTVAFNHFG 274

Query: 425 EGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHE 484
           EGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTI SQGNRF+AP  ++RKEVTKHE
Sbjct: 275 EGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAAPTINSQGNRFLAPNEKYRKEVTKHE 334

Query: 485 DAPESEWRHWNWRSEGDLLLNGAYFRQSGAGASSS--YARAYSLSARPSSLVGDITITAG 544
           DAPES+WR WNWRSEGDL+LNGAYFRQ+GAGASSS  YARA SLSARPSSLVG IT  AG
Sbjct: 335 DAPESQWRSWNWRSEGDLMLNGAYFRQTGAGASSSSTYARASSLSARPSSLVGSITTNAG 394

Query: 545 ALNCRRGSRC 551
            +NC++GSRC
Sbjct: 395 PVNCKKGSRC 404

BLAST of Chy9G161010 vs. ExPASy Swiss-Prot
Match: Q93Z25 (Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 1.5e-180
Identity = 289/364 (79.40%), Postives = 326/364 (89.56%), Query Frame = 0

Query: 189 SRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVTDSGN 248
           SRR LG+ SCG+GNPIDDCWRCD +WE NRKRLADC IGFGKNAIGG++G  YVVTD GN
Sbjct: 69  SRRKLGFFSCGSGNPIDDCWRCDKDWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGN 128

Query: 249 DDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAGGPCI 308
           DDP+NPRPGTLR+AVIQ+EPLWIIFKRDM I+L +EL+MNSFKT+DGRGASVH++GGPCI
Sbjct: 129 DDPVNPRPGTLRYAVIQDEPLWIIFKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCI 188

Query: 309 KIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDHCSLS 368
            I Y TNIIIHG+HIHDCK+GGN  +RDSP+H G+ T SDGDGVSI GG  +W+DHCSLS
Sbjct: 189 TIQYVTNIIIHGLHIHDCKQGGNTYVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLS 248

Query: 369 NCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEGLVQR 428
           NCNDGLIDAI GSTAITISNNY+THH+KVMLLGHSD++ QDKNMQVTIAFNHFGEGLVQR
Sbjct: 249 NCNDGLIDAIRGSTAITISNNYLTHHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQR 308

Query: 429 MPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDAPESE 488
           MPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTI SQGNRF+AP +   KEVTKHEDAPE E
Sbjct: 309 MPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDE 368

Query: 489 WRHWNWRSEGDLLLNGAYFRQSGAG--ASSSYARAYSLSARPSSLVGDITITAGALNCRR 548
           WR+WNWRSEGDLLLNGA+F  SGAG   SSSY++A SL+ARPSS VG+ITI +GAL+C+R
Sbjct: 369 WRNWNWRSEGDLLLNGAFFTYSGAGPAKSSSYSKASSLAARPSSHVGEITIASGALSCKR 428

Query: 549 GSRC 551
           GS C
Sbjct: 429 GSHC 432

BLAST of Chy9G161010 vs. ExPASy TrEMBL
Match: A0A0A0KNX3 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_5G517820 PE=3 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 4.9e-222
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0

Query: 185 SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT 244
           SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT
Sbjct: 53  SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT 112

Query: 245 DSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAG 304
           DSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAG
Sbjct: 113 DSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAG 172

Query: 305 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH 364
           GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH
Sbjct: 173 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH 232

Query: 365 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG 424
           CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG
Sbjct: 233 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG 292

Query: 425 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA 484
           LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA
Sbjct: 293 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA 352

Query: 485 PESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNC 544
           PESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNC
Sbjct: 353 PESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNC 412

Query: 545 RRGSRC 551
           RRGSRC
Sbjct: 413 RRGSRC 418

BLAST of Chy9G161010 vs. ExPASy TrEMBL
Match: A0A5D3CGC7 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold249G00560 PE=3 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 9.6e-218
Identity = 357/366 (97.54%), Postives = 363/366 (99.18%), Query Frame = 0

Query: 185 SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT 244
           SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT
Sbjct: 29  SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT 88

Query: 245 DSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAG 304
           D+ +DDPLNPRPGTLRHAVIQNEPLWIIFKRDMVI+L QELVMNSFKTIDGRGASVH+AG
Sbjct: 89  DARDDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIQLEQELVMNSFKTIDGRGASVHVAG 148

Query: 305 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH 364
           GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH
Sbjct: 149 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH 208

Query: 365 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG 424
           CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG
Sbjct: 209 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG 268

Query: 425 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA 484
           LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA
Sbjct: 269 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA 328

Query: 485 PESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNC 544
           PESEWRHWNWRSEGD+LLNGAYFRQSGAGA SSYARAYSLSARPSSLVGDIT+TAGALNC
Sbjct: 329 PESEWRHWNWRSEGDMLLNGAYFRQSGAGARSSYARAYSLSARPSSLVGDITLTAGALNC 388

Query: 545 RRGSRC 551
           RRGSRC
Sbjct: 389 RRGSRC 394

BLAST of Chy9G161010 vs. ExPASy TrEMBL
Match: A0A1S3C8C5 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103498129 PE=3 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 4.8e-217
Identity = 356/366 (97.27%), Postives = 363/366 (99.18%), Query Frame = 0

Query: 185 SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT 244
           SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT
Sbjct: 52  SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT 111

Query: 245 DSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAG 304
           D+ +DDPLNPRPGTLRHAVIQNEPLWIIFKRDMVI+L QELVMNSFKTIDGRGASVH+AG
Sbjct: 112 DARDDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIQLEQELVMNSFKTIDGRGASVHVAG 171

Query: 305 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH 364
           GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH
Sbjct: 172 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH 231

Query: 365 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG 424
           CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG
Sbjct: 232 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG 291

Query: 425 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA 484
           LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA
Sbjct: 292 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA 351

Query: 485 PESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNC 544
           PESEWR+WNWRSEGD+LLNGAYFRQSGAGA SSYARAYSLSARPSSLVGDIT+TAGALNC
Sbjct: 352 PESEWRYWNWRSEGDMLLNGAYFRQSGAGARSSYARAYSLSARPSSLVGDITLTAGALNC 411

Query: 545 RRGSRC 551
           RRGSRC
Sbjct: 412 RRGSRC 417

BLAST of Chy9G161010 vs. ExPASy TrEMBL
Match: A0A6J1H707 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111460762 PE=3 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 2.6e-207
Identity = 340/368 (92.39%), Postives = 354/368 (96.20%), Query Frame = 0

Query: 185 SINGS--RRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYV 244
           SIN S  RRNLGYLSC TGNPIDDCWRCD NWETNRKRLADCAIGFGKNAIGG+NGRFYV
Sbjct: 90  SINESRTRRNLGYLSCRTGNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYV 149

Query: 245 VTDSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHL 304
           VTD+G+DDPLNPRPGTLRHAVIQNEPLWIIFKRDMVI+L QELVMNSFKTIDGRGASVH+
Sbjct: 150 VTDAGDDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHV 209

Query: 305 AGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWI 364
           AGGPCI IHYATNIIIHGIHIHDCKRGGN NIRDSPQH+GWWT SDGDGVSI GGK IW+
Sbjct: 210 AGGPCITIHYATNIIIHGIHIHDCKRGGNGNIRDSPQHSGWWTISDGDGVSIFGGKNIWV 269

Query: 365 DHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFG 424
           DHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDS+TQDKNMQVTIAFNHFG
Sbjct: 270 DHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFG 329

Query: 425 EGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHE 484
           EGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRF+AP  R+RKEVTKHE
Sbjct: 330 EGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFLAPDVRYRKEVTKHE 389

Query: 485 DAPESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGAL 544
           DAPESEWRHWNWRSEGD++LNGAYFRQSGAGA+SSYARAYSLSARPSSLVG IT+TAGAL
Sbjct: 390 DAPESEWRHWNWRSEGDMMLNGAYFRQSGAGAASSYARAYSLSARPSSLVGSITVTAGAL 449

Query: 545 NCRRGSRC 551
           NCRRGSRC
Sbjct: 450 NCRRGSRC 457

BLAST of Chy9G161010 vs. ExPASy TrEMBL
Match: A0A6J1JPY5 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111487839 PE=3 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.7e-206
Identity = 338/368 (91.85%), Postives = 354/368 (96.20%), Query Frame = 0

Query: 185 SINGS--RRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYV 244
           SIN S  RRNLGYLSC TGNPIDDCWRCD NWETNRKRLADCAIGFGKNAIGG+NGRFY+
Sbjct: 56  SINESRIRRNLGYLSCRTGNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYM 115

Query: 245 VTDSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHL 304
           VTD+G+DDPLNPRPGTLRHAVIQNEPLWIIFKRDMVI+L QELVMNSFKTIDGRGASVH+
Sbjct: 116 VTDAGDDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHV 175

Query: 305 AGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWI 364
           AGGPCI IHYATNIIIHGIHIHDCKRGGN NIRDSPQH+GWWT SDGDGVSI GGK IW+
Sbjct: 176 AGGPCITIHYATNIIIHGIHIHDCKRGGNGNIRDSPQHSGWWTISDGDGVSIFGGKNIWV 235

Query: 365 DHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFG 424
           DHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDS+TQDKNMQVTIAFNHFG
Sbjct: 236 DHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFG 295

Query: 425 EGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHE 484
           EGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRF+AP  R++KEVTKHE
Sbjct: 296 EGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFLAPDVRYQKEVTKHE 355

Query: 485 DAPESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGAL 544
           DAPESEWRHWNWRSEGD++LNGAYFRQSGAGA+SSYARAYSLSARPSSLVG IT+TAGAL
Sbjct: 356 DAPESEWRHWNWRSEGDMMLNGAYFRQSGAGAASSYARAYSLSARPSSLVGSITVTAGAL 415

Query: 545 NCRRGSRC 551
           NCRRGSRC
Sbjct: 416 NCRRGSRC 423

BLAST of Chy9G161010 vs. NCBI nr
Match: XP_004142773.1 (pectate lyase [Cucumis sativus] >KGN51335.1 hypothetical protein Csa_008191 [Cucumis sativus])

HSP 1 Score: 776 bits (2003), Expect = 1.24e-279
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0

Query: 185 SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT 244
           SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT
Sbjct: 53  SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT 112

Query: 245 DSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAG 304
           DSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAG
Sbjct: 113 DSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAG 172

Query: 305 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH 364
           GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH
Sbjct: 173 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH 232

Query: 365 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG 424
           CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG
Sbjct: 233 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG 292

Query: 425 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA 484
           LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA
Sbjct: 293 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA 352

Query: 485 PESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNC 544
           PESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNC
Sbjct: 353 PESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNC 412

Query: 545 RRGSRC 550
           RRGSRC
Sbjct: 413 RRGSRC 418

BLAST of Chy9G161010 vs. NCBI nr
Match: KAA0037617.1 (pectate lyase-like [Cucumis melo var. makuwa] >TYK09359.1 pectate lyase-like [Cucumis melo var. makuwa])

HSP 1 Score: 761 bits (1966), Expect = 2.15e-274
Identity = 357/366 (97.54%), Postives = 363/366 (99.18%), Query Frame = 0

Query: 185 SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT 244
           SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT
Sbjct: 29  SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT 88

Query: 245 DSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAG 304
           D+ +DDPLNPRPGTLRHAVIQNEPLWIIFKRDMVI+L QELVMNSFKTIDGRGASVH+AG
Sbjct: 89  DARDDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIQLEQELVMNSFKTIDGRGASVHVAG 148

Query: 305 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH 364
           GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH
Sbjct: 149 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH 208

Query: 365 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG 424
           CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG
Sbjct: 209 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG 268

Query: 425 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA 484
           LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA
Sbjct: 269 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA 328

Query: 485 PESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNC 544
           PESEWRHWNWRSEGD+LLNGAYFRQSGAGA SSYARAYSLSARPSSLVGDIT+TAGALNC
Sbjct: 329 PESEWRHWNWRSEGDMLLNGAYFRQSGAGARSSYARAYSLSARPSSLVGDITLTAGALNC 388

Query: 545 RRGSRC 550
           RRGSRC
Sbjct: 389 RRGSRC 394

BLAST of Chy9G161010 vs. NCBI nr
Match: XP_008458848.1 (PREDICTED: pectate lyase-like [Cucumis melo])

HSP 1 Score: 759 bits (1960), Expect = 4.18e-273
Identity = 356/366 (97.27%), Postives = 363/366 (99.18%), Query Frame = 0

Query: 185 SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT 244
           SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT
Sbjct: 52  SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT 111

Query: 245 DSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAG 304
           D+ +DDPLNPRPGTLRHAVIQNEPLWIIFKRDMVI+L QELVMNSFKTIDGRGASVH+AG
Sbjct: 112 DARDDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIQLEQELVMNSFKTIDGRGASVHVAG 171

Query: 305 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH 364
           GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH
Sbjct: 172 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH 231

Query: 365 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG 424
           CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG
Sbjct: 232 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG 291

Query: 425 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA 484
           LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA
Sbjct: 292 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA 351

Query: 485 PESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNC 544
           PESEWR+WNWRSEGD+LLNGAYFRQSGAGA SSYARAYSLSARPSSLVGDIT+TAGALNC
Sbjct: 352 PESEWRYWNWRSEGDMLLNGAYFRQSGAGARSSYARAYSLSARPSSLVGDITLTAGALNC 411

Query: 545 RRGSRC 550
           RRGSRC
Sbjct: 412 RRGSRC 417

BLAST of Chy9G161010 vs. NCBI nr
Match: XP_038890725.1 (pectate lyase-like [Benincasa hispida])

HSP 1 Score: 741 bits (1912), Expect = 7.84e-266
Identity = 343/366 (93.72%), Postives = 357/366 (97.54%), Query Frame = 0

Query: 185 SINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVT 244
           SIN SRRNLGY SCGTGNPIDDCWRCDSNW TNRKRLADCAIGFGKNAIGGKNGRFYVVT
Sbjct: 50  SINASRRNLGYFSCGTGNPIDDCWRCDSNWATNRKRLADCAIGFGKNAIGGKNGRFYVVT 109

Query: 245 DSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAG 304
           D+G+DDP+NPRPGTLRHAVIQNEPLWIIFKRDMVI+L QELVMNSFKTIDGRGASVH+AG
Sbjct: 110 DAGDDDPVNPRPGTLRHAVIQNEPLWIIFKRDMVIQLTQELVMNSFKTIDGRGASVHVAG 169

Query: 305 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDH 364
           GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQH+GWWTASDGDGVSILGG++IWIDH
Sbjct: 170 GPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHSGWWTASDGDGVSILGGREIWIDH 229

Query: 365 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEG 424
           CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDS+ QDKNMQVTIAFNHFGEG
Sbjct: 230 CSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYAQDKNMQVTIAFNHFGEG 289

Query: 425 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDA 484
           LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRF APYNRFRKEVTKHEDA
Sbjct: 290 LVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFAAPYNRFRKEVTKHEDA 349

Query: 485 PESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNC 544
           PESEWRHWNWRSEGD++LNGAYFRQSGAGA+SSYARA SLSARPSSLV  IT+TAGALNC
Sbjct: 350 PESEWRHWNWRSEGDMMLNGAYFRQSGAGAASSYARANSLSARPSSLVSSITVTAGALNC 409

Query: 545 RRGSRC 550
           R+GSRC
Sbjct: 410 RKGSRC 415

BLAST of Chy9G161010 vs. NCBI nr
Match: KAG6602397.1 (putative pectate lyase 11, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033073.1 putative pectate lyase 11, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 727 bits (1876), Expect = 1.62e-260
Identity = 340/368 (92.39%), Postives = 354/368 (96.20%), Query Frame = 0

Query: 185 SINGSR--RNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYV 244
           SIN SR  RNLGYLSC TGNPIDDCWRCD NWETNRKRLADCAIGFGKNAIGG+NGRFYV
Sbjct: 38  SINESRTRRNLGYLSCRTGNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYV 97

Query: 245 VTDSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHL 304
           VTD+G+DDPLNPRPGTLRHAVIQNEPLWIIFKRDMVI+L QELVMNSFKTIDGRGASVH+
Sbjct: 98  VTDAGDDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHV 157

Query: 305 AGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWI 364
           AGGPCI IHYATNIIIHGIHIHDCKRGGN NIRDSPQH+GWWT SDGDGVSI GGK IW+
Sbjct: 158 AGGPCITIHYATNIIIHGIHIHDCKRGGNGNIRDSPQHSGWWTISDGDGVSIFGGKNIWV 217

Query: 365 DHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFG 424
           DHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDS+TQDKNMQVTIAFNHFG
Sbjct: 218 DHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFG 277

Query: 425 EGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHE 484
           EGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRF+AP  R+RKEVTKHE
Sbjct: 278 EGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFLAPDVRYRKEVTKHE 337

Query: 485 DAPESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGAL 544
           DAPESEWRHWNWRSEGD++LNGAYFRQSGAGA+SSYARAYSLSARPSSLVG IT+TAGAL
Sbjct: 338 DAPESEWRHWNWRSEGDMMLNGAYFRQSGAGAASSYARAYSLSARPSSLVGSITVTAGAL 397

Query: 545 NCRRGSRC 550
           NCRRGSRC
Sbjct: 398 NCRRGSRC 405

BLAST of Chy9G161010 vs. TAIR 10
Match: AT3G27400.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 658.3 bits (1697), Expect = 5.4e-189
Identity = 301/364 (82.69%), Postives = 326/364 (89.56%), Query Frame = 0

Query: 187 NGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVTDS 246
           N SRR+L YLSC TGNPIDDCWRCD NWETNR+RLADCAIGFGKNAIGG+ GR YVVTD 
Sbjct: 49  NASRRSLAYLSCRTGNPIDDCWRCDPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDP 108

Query: 247 GNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAGGP 306
            NDDP+NPRPGTLR+AV Q EPLWIIFKRDMVI+L +EL++ SFKTIDGRG+SVH+  GP
Sbjct: 109 ANDDPVNPRPGTLRYAVTQEEPLWIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGP 168

Query: 307 CIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDHCS 366
           C+KIHYATNIIIHGI+IHDCK G    I+D P H GWW  SDGD V+I GGK +WIDHCS
Sbjct: 169 CLKIHYATNIIIHGINIHDCKPGSGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCS 228

Query: 367 LSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEGLV 426
           LSNC+DGLIDAIHGSTAITISNN+MTHHDKVMLLGHSDS+TQDKNMQVTIAFNHFGEGLV
Sbjct: 229 LSNCDDGLIDAIHGSTAITISNNHMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLV 288

Query: 427 QRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDAPE 486
           QRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRF+AP  RF KEVTKHEDAPE
Sbjct: 289 QRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPE 348

Query: 487 SEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNCRR 546
           S+WR WNWRSEGD+LLNGAYFR+SGA A S+YARA SLSARPSSLVG IT TAG L+CRR
Sbjct: 349 SKWRDWNWRSEGDMLLNGAYFRESGAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRR 408

Query: 547 GSRC 551
           G RC
Sbjct: 409 GRRC 412

BLAST of Chy9G161010 vs. TAIR 10
Match: AT4G24780.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 639.8 bits (1649), Expect = 2.0e-183
Identity = 293/369 (79.40%), Postives = 330/369 (89.43%), Query Frame = 0

Query: 185 SINGS---RRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFY 244
           SIN S   RR LGYLSC TGNPIDDCWRCD +WE +R+RLADCAIGFGKNAIGG++GR Y
Sbjct: 40  SINASVAGRRKLGYLSCTTGNPIDDCWRCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIY 99

Query: 245 VVTDSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVH 304
           VVTDSGND+P++P+PGTLRHAV+Q+EPLWIIF+RDM I+L +EL+MNSFKTIDGRGASVH
Sbjct: 100 VVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKEELIMNSFKTIDGRGASVH 159

Query: 305 LAGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIW 364
           ++GGPCI I Y TNIIIHGIHIHDCK+GGNA +R SP+H GW T SDGDGVSI GG  +W
Sbjct: 160 ISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVW 219

Query: 365 IDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHF 424
           +DHCS SNC DGLIDAI GSTAIT+SNN+MTHHDKVMLLGHSD++++DKNMQVTIAFNHF
Sbjct: 220 VDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHF 279

Query: 425 GEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKH 484
           GEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTI SQGNRF+AP  RF KEVTKH
Sbjct: 280 GEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPNIRFSKEVTKH 339

Query: 485 EDAPESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGA 544
           EDAPESEW+ WNWRS GDLLLNGA+F  SG  ASSSYA+A SL A+PSSLVG +T T+GA
Sbjct: 340 EDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASSLGAKPSSLVGPLTSTSGA 399

Query: 545 LNCRRGSRC 551
           LNCR+GSRC
Sbjct: 400 LNCRKGSRC 408

BLAST of Chy9G161010 vs. TAIR 10
Match: AT4G24780.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 639.8 bits (1649), Expect = 2.0e-183
Identity = 293/369 (79.40%), Postives = 330/369 (89.43%), Query Frame = 0

Query: 185 SINGS---RRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFY 244
           SIN S   RR LGYLSC TGNPIDDCWRCD +WE +R+RLADCAIGFGKNAIGG++GR Y
Sbjct: 40  SINASVAGRRKLGYLSCTTGNPIDDCWRCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIY 99

Query: 245 VVTDSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVH 304
           VVTDSGND+P++P+PGTLRHAV+Q+EPLWIIF+RDM I+L +EL+MNSFKTIDGRGASVH
Sbjct: 100 VVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKEELIMNSFKTIDGRGASVH 159

Query: 305 LAGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIW 364
           ++GGPCI I Y TNIIIHGIHIHDCK+GGNA +R SP+H GW T SDGDGVSI GG  +W
Sbjct: 160 ISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVW 219

Query: 365 IDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHF 424
           +DHCS SNC DGLIDAI GSTAIT+SNN+MTHHDKVMLLGHSD++++DKNMQVTIAFNHF
Sbjct: 220 VDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHF 279

Query: 425 GEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKH 484
           GEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTI SQGNRF+AP  RF KEVTKH
Sbjct: 280 GEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPNIRFSKEVTKH 339

Query: 485 EDAPESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGA 544
           EDAPESEW+ WNWRS GDLLLNGA+F  SG  ASSSYA+A SL A+PSSLVG +T T+GA
Sbjct: 340 EDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASSLGAKPSSLVGPLTSTSGA 399

Query: 545 LNCRRGSRC 551
           LNCR+GSRC
Sbjct: 400 LNCRKGSRC 408

BLAST of Chy9G161010 vs. TAIR 10
Match: AT5G63180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 634.0 bits (1634), Expect = 1.1e-181
Identity = 289/364 (79.40%), Postives = 326/364 (89.56%), Query Frame = 0

Query: 189 SRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVTDSGN 248
           SRR LG+ SCG+GNPIDDCWRCD +WE NRKRLADC IGFGKNAIGG++G  YVVTD GN
Sbjct: 69  SRRKLGFFSCGSGNPIDDCWRCDKDWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGN 128

Query: 249 DDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAGGPCI 308
           DDP+NPRPGTLR+AVIQ+EPLWIIFKRDM I+L +EL+MNSFKT+DGRGASVH++GGPCI
Sbjct: 129 DDPVNPRPGTLRYAVIQDEPLWIIFKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCI 188

Query: 309 KIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDHCSLS 368
            I Y TNIIIHG+HIHDCK+GGN  +RDSP+H G+ T SDGDGVSI GG  +W+DHCSLS
Sbjct: 189 TIQYVTNIIIHGLHIHDCKQGGNTYVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLS 248

Query: 369 NCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEGLVQR 428
           NCNDGLIDAI GSTAITISNNY+THH+KVMLLGHSD++ QDKNMQVTIAFNHFGEGLVQR
Sbjct: 249 NCNDGLIDAIRGSTAITISNNYLTHHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQR 308

Query: 429 MPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDAPESE 488
           MPRCRHGYFHVVNNDYTHWEMYAIGGSA+PTI SQGNRF+AP +   KEVTKHEDAPE E
Sbjct: 309 MPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDE 368

Query: 489 WRHWNWRSEGDLLLNGAYFRQSGAG--ASSSYARAYSLSARPSSLVGDITITAGALNCRR 548
           WR+WNWRSEGDLLLNGA+F  SGAG   SSSY++A SL+ARPSS VG+ITI +GAL+C+R
Sbjct: 369 WRNWNWRSEGDLLLNGAFFTYSGAGPAKSSSYSKASSLAARPSSHVGEITIASGALSCKR 428

Query: 549 GSRC 551
           GS C
Sbjct: 429 GSHC 432

BLAST of Chy9G161010 vs. TAIR 10
Match: AT1G67750.1 (Pectate lyase family protein )

HSP 1 Score: 629.0 bits (1621), Expect = 3.5e-180
Identity = 290/369 (78.59%), Postives = 324/369 (87.80%), Query Frame = 0

Query: 182 VGLSINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFY 241
           V   IN SRRNLG LSCGTGNPIDDCWRCD  WE NR+RLADCAIGFGK+AIGG++G+ Y
Sbjct: 40  VNEKINASRRNLGVLSCGTGNPIDDCWRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIY 99

Query: 242 VVTDSGNDDPLNPRPGTLRHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVH 301
           VVTDS + D +NP+PGTLRHAVIQ+EPLWIIF RDMVIKL +EL+MNSFKTIDGRGASVH
Sbjct: 100 VVTDSSDKDVVNPKPGTLRHAVIQDEPLWIIFARDMVIKLKEELIMNSFKTIDGRGASVH 159

Query: 302 LAGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIW 361
           +AGG CI + Y TNIIIHG++IHDCKR GNA +RDSP H GW TASDGD VSI GG  +W
Sbjct: 160 IAGGACITVQYVTNIIIHGVNIHDCKRKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVW 219

Query: 362 IDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHF 421
           +DHCSLSNC DGLIDAIHGSTAITISNNY++HH+KVMLLGHSDS+T+DKNMQVTIAFNHF
Sbjct: 220 VDHCSLSNCADGLIDAIHGSTAITISNNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHF 279

Query: 422 GEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKH 481
           GEGLVQRMPRCRHGYFHVVNNDYTHW+MYAIGGSA+PTI SQGNRF+AP +   KEVTK+
Sbjct: 280 GEGLVQRMPRCRHGYFHVVNNDYTHWQMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKY 339

Query: 482 EDAPESEWRHWNWRSEGDLLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGA 541
           EDAP S+W+ WNWRSEGDL LNGA+F  SG GASSSYA+A SLSARPSSLV  +T  AGA
Sbjct: 340 EDAPRSKWKKWNWRSEGDLFLNGAFFTPSGGGASSSYAKASSLSARPSSLVASVTSNAGA 399

Query: 542 LNCRRGSRC 551
           L CR+GSRC
Sbjct: 400 LFCRKGSRC 408

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O245541.4e-18983.33Pectate lyase OS=Zinnia violacea OX=34245 PE=1 SV=1[more]
Q9LTZ07.7e-18882.69Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2[more]
Q9C5M82.8e-18279.40Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2[more]
P243969.1e-18180.54Probable pectate lyase P18 OS=Solanum lycopersicum OX=4081 GN=9612 PE=2 SV=1[more]
Q93Z251.5e-18079.40Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KNX34.9e-222100.00Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_5G517820 PE=3 SV=1[more]
A0A5D3CGC79.6e-21897.54Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold249G00560 ... [more]
A0A1S3C8C54.8e-21797.27Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103498129 PE=3 SV=1[more]
A0A6J1H7072.6e-20792.39Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111460762 PE=3 SV=1[more]
A0A6J1JPY51.7e-20691.85Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111487839 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004142773.11.24e-279100.00pectate lyase [Cucumis sativus] >KGN51335.1 hypothetical protein Csa_008191 [Cuc... [more]
KAA0037617.12.15e-27497.54pectate lyase-like [Cucumis melo var. makuwa] >TYK09359.1 pectate lyase-like [Cu... [more]
XP_008458848.14.18e-27397.27PREDICTED: pectate lyase-like [Cucumis melo][more]
XP_038890725.17.84e-26693.72pectate lyase-like [Benincasa hispida][more]
KAG6602397.11.62e-26092.39putative pectate lyase 11, partial [Cucurbita argyrosperma subsp. sororia] >KAG7... [more]
Match NameE-valueIdentityDescription
AT3G27400.15.4e-18982.69Pectin lyase-like superfamily protein [more]
AT4G24780.12.0e-18379.40Pectin lyase-like superfamily protein [more]
AT4G24780.22.0e-18379.40Pectin lyase-like superfamily protein [more]
AT5G63180.11.1e-18179.40Pectin lyase-like superfamily protein [more]
AT1G67750.13.5e-18078.59Pectate lyase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 248..273
score: 66.3
coord: 521..544
score: 39.68
coord: 492..516
score: 54.86
coord: 348..369
score: 59.09
coord: 224..241
score: 62.7
coord: 284..300
score: 71.43
coord: 428..447
score: 78.1
coord: 450..469
score: 77.86
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 276..473
e-value: 1.3E-103
score: 360.2
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 285..467
e-value: 3.0E-20
score: 72.7
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 201..543
e-value: 5.1E-115
score: 386.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..163
NoneNo IPR availablePANTHERPTHR31683:SF147PECTATE LYASEcoord: 184..547
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 184..547
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 183..544

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy9G161010.1Chy9G161010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity