Chy9G159640 (gene) Cucumber (hystrix) v1

Overview


Name	Chy9G159640
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	E3 ubiquitin-protein ligase RING1-like
Location	chrH09: 2041208 .. 2041918 (-)
RNA-Seq Expression	Chy9G159640
Synteny	Chy9G159640

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGATTCCGATTCTTCTCCCTCTCCTTCACCGCCACCGCCTCCGCCTCCTCCTCCGCGGACCCTTTCTATGCTTCTTCCTTTTCTCCTTGGCCTTGCTCGAACTACCACCGGATCCAACCTCAACCAGATCGTCGTTCTCGACCATGCTGCGCGAACGGTTGTCGTTTTCGAAGGACACGTTCTTGATTCTCTTCTTCGTGACTTATCGGAGAAATCTGGGCCGTTGCCGGCGTCCAAGGCTTCGATCGATGCGATGCCTCGAGTTGGGGTTACGGAATTGGGGATGGATTGTGCGATTTGCTTGGATGGATTTGAAGTGGACGAGGAAGCTAGGGAGATGCCGTGTAAACATATGTATCATTCTGATTGTATTGAGAAATGGTTGAATGTTCGTGGAACCTGTCCGGTTTGTCGGTTCGTGATGCCGGTGGATGATCGGATAAAGGACGGTGGTGGTGGTGATGAAGAAGAAGAGGAAGATATGGAAGGTGGAGAAGGTGGTGAAGGACGAGTGGTTGTAGTTAATTTGTGGGTTAACGATGGTGAACCGATGGATGTTGAGTCGGGATTGGAACATGGATCAGAGGATTCCGATTCAGAATCACAAGATGAAATTATGGTTGAAGTTGATGGCTCATCAACACAAGAACAAGATCCAAGTTCTGAAGATTCTTCTTCATTTTCATTTTCGTCTTTTTTCAACTAG

mRNA sequence

ATGTCTGATTCCGATTCTTCTCCCTCTCCTTCACCGCCACCGCCTCCGCCTCCTCCTCCGCGGACCCTTTCTATGCTTCTTCCTTTTCTCCTTGGCCTTGCTCGAACTACCACCGGATCCAACCTCAACCAGATCGTCGTTCTCGACCATGCTGCGCGAACGGTTGTCGTTTTCGAAGGACACGTTCTTGATTCTCTTCTTCGTGACTTATCGGAGAAATCTGGGCCGTTGCCGGCGTCCAAGGCTTCGATCGATGCGATGCCTCGAGTTGGGGTTACGGAATTGGGGATGGATTGTGCGATTTGCTTGGATGGATTTGAAGTGGACGAGGAAGCTAGGGAGATGCCGTGTAAACATATGTATCATTCTGATTGTATTGAGAAATGGTTGAATGTTCGTGGAACCTGTCCGGTTTGTCGGTTCGTGATGCCGGTGGATGATCGGATAAAGGACGGTGGTGGTGGTGATGAAGAAGAAGAGGAAGATATGGAAGGTGGAGAAGGTGGTGAAGGACGAGTGGTTGTAGTTAATTTGTGGGTTAACGATGGTGAACCGATGGATGTTGAGTCGGGATTGGAACATGGATCAGAGGATTCCGATTCAGAATCACAAGATGAAATTATGGTTGAAGTTGATGGCTCATCAACACAAGAACAAGATCCAAGTTCTGAAGATTCTTCTTCATTTTCATTTTCGTCTTTTTTCAACTAG

Coding sequence (CDS)

ATGTCTGATTCCGATTCTTCTCCCTCTCCTTCACCGCCACCGCCTCCGCCTCCTCCTCCGCGGACCCTTTCTATGCTTCTTCCTTTTCTCCTTGGCCTTGCTCGAACTACCACCGGATCCAACCTCAACCAGATCGTCGTTCTCGACCATGCTGCGCGAACGGTTGTCGTTTTCGAAGGACACGTTCTTGATTCTCTTCTTCGTGACTTATCGGAGAAATCTGGGCCGTTGCCGGCGTCCAAGGCTTCGATCGATGCGATGCCTCGAGTTGGGGTTACGGAATTGGGGATGGATTGTGCGATTTGCTTGGATGGATTTGAAGTGGACGAGGAAGCTAGGGAGATGCCGTGTAAACATATGTATCATTCTGATTGTATTGAGAAATGGTTGAATGTTCGTGGAACCTGTCCGGTTTGTCGGTTCGTGATGCCGGTGGATGATCGGATAAAGGACGGTGGTGGTGGTGATGAAGAAGAAGAGGAAGATATGGAAGGTGGAGAAGGTGGTGAAGGACGAGTGGTTGTAGTTAATTTGTGGGTTAACGATGGTGAACCGATGGATGTTGAGTCGGGATTGGAACATGGATCAGAGGATTCCGATTCAGAATCACAAGATGAAATTATGGTTGAAGTTGATGGCTCATCAACACAAGAACAAGATCCAAGTTCTGAAGATTCTTCTTCATTTTCATTTTCGTCTTTTTTCAACTAG

Protein sequence

MSDSDSSPSPSPPPPPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEGHVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHMYHSDCIEKWLNVRGTCPVCRFVMPVDDRIKDGGGGDEEEEEDMEGGEGGEGRVVVVNLWVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN*

Homology

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BLAST of Chy9G159640 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 6.7e-24
Identity = 71/189 (37.57%), Postives = 102/189 (53.97%), Query Frame = 0


Query: 21  RTLSMLLPFLLGLARTTT--------GSNLNQIVVLDHAARTVVVFEGHV-LDSLLRDL- 80
           R L + LPFLLG +   +         S+  +I++++   + ++V EG   ++ LLR L 
Sbjct: 24  RDLYLFLPFLLGFSDQESSNGDDDDVASSRERIILVNPFTQGMIVLEGSSGMNPLLRSLL 83

Query: 81  -SEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHMYHSDCIEKW 140
            S + G  PASKASIDAMP V +     +C ICL+ ++ +E  +EMPCKH +H  CIEKW
Sbjct: 84  ESREEGRPPASKASIDAMPIVEIDGCEGECVICLEEWKSEETVKEMPCKHRFHGGCIEKW 143

Query: 141 LNVRGTCPVCRFVMPVDDRIKDGGGGDEEEEEDMEGGEGGEGRVVVVNLWVNDGEPMDVE 199
           L   G+CPVCR+ MPVD        GDE       G +  +G  + V    NDG  +   
Sbjct: 144 LGFHGSCPVCRYEMPVD--------GDE------IGKKRNDGNEIWVRFSFNDGRRIRDF 198

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BLAST of Chy9G159640 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 8.5e-19
Identity = 44/103 (42.72%), Postives = 64/103 (62.14%), Query Frame = 0


Query: 57  VFEGHVLDSLLRDLSE---------KSGPLPASKASIDAMPRVGV----TELGMDCAICL 116
           +  G   + LL  LS+         +SG  PASK++I+++PRV +    T+   +CA+C 
Sbjct: 160 ILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDCHTKAEANCAVCT 219

Query: 117 DGFEVDEEAREMPCKHMYHSDCIEKWLNVRGTCPVCRFVMPVD 147
           + FE   E REMPCKH++H DCI  WL++R +CPVCRF +P D
Sbjct: 220 EVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELPSD 262

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BLAST of Chy9G159640 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.5e-18
Identity = 50/132 (37.88%), Postives = 76/132 (57.58%), Query Frame = 0


Query: 58  FEGHVLDSLLRDLSE----KSGPLPASKASIDAMPRVGVTELGMD-----CAICLDGFEV 117
           F G  L+ L++ L+E    + G  PASK++I+A+P V +T+  ++     CA+C+D FE 
Sbjct: 174 FIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEE 233

Query: 118 DEEAREMPCKHMYHSDCIEKWLNVRGTCPVCRFVMPVDD-----RIKDGGGGDEEEEEDM 175
             EA++MPCKH+YH DC+  WL +  +CPVCR  +P DD     R++   G     + D 
Sbjct: 234 GTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTSGGNDGDN 293

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BLAST of Chy9G159640 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.5e-18
Identity = 53/146 (36.30%), Postives = 82/146 (56.16%), Query Frame = 0


Query: 57  VFEGHVLDSLLRDLSE---------KSGPLPASKASIDAMPRVGVTELGM----DCAICL 116
           +  G   + LL  LS+         +SG  PASK++I+++PRV +++  +    +CA+C 
Sbjct: 144 ILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCHIGSEANCAVCT 203

Query: 117 DGFEVDEEAREMPCKHMYHSDCIEKWLNVRGTCPVCRFVMPVDDRIKDGGGGDEEEEEDM 176
           + FE + EAREMPCKH++H DCI  WL++R +CPVCRF +P +   +     +  EE++ 
Sbjct: 204 EIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRR----SNNNEEDNA 263

Query: 177 EGG-----EGGEGRVVVVNLWVNDGE 185
            G       GG   V   N  + DGE
Sbjct: 264 VGMTIWRLPGGGFAVGRFNAAMRDGE 285

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BLAST of Chy9G159640 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 9.4e-18
Identity = 48/125 (38.40%), Postives = 72/125 (57.60%), Query Frame = 0


Query: 58  FEGHVLDSLLRDLSE----KSGPLPASKASIDAMPRVGVTELGM-----DCAICLDGFEV 117
           F G  L+ L++ L+E    + G  PASK++IDA+P V VT+  +      CA+C+D FE 
Sbjct: 166 FFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFED 225

Query: 118 DEEAREMPCKHMYHSDCIEKWLNVRGTCPVCRFVMPVDDRIKDGGGGDEEEEEDMEGGEG 174
             + ++MPCKH++H DC+  WL +  +CPVCRF +P DD         E   +  +G   
Sbjct: 226 GSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDD------PDYENRSQGSQGSGD 284

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BLAST of Chy9G159640 vs. ExPASy TrEMBL
Match: A0A5A7T7P7 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00210 PE=4 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 3.5e-116
Identity = 226/238 (94.96%), Postives = 227/238 (95.38%), Query Frame = 0


Query: 1   MSDSDSSPSPSPPPPPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60
           MSDSDSSPSPS    PPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG
Sbjct: 1   MSDSDSSPSPS----PPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60

Query: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHM 120
           HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELG DCAICLDGFEV EEAREMPCKHM
Sbjct: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGTDCAICLDGFEVAEEAREMPCKHM 120

Query: 121 YHSDCIEKWLNVRGTCPVCRFVMPVDDRIKDG--GGGDEEEEEDMEGGEGGEGRVVVVNL 180
           YHSDCIEKWLNVRGTCPVCRFVMPVDDR KDG  GGGD EE+EDMEGGE GEGRVVVVNL
Sbjct: 121 YHSDCIEKWLNVRGTCPVCRFVMPVDDRRKDGGVGGGDVEEDEDMEGGESGEGRVVVVNL 180

Query: 181 WVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN 237
           WVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 WVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN 234

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BLAST of Chy9G159640 vs. ExPASy TrEMBL
Match: A0A0A0KS87 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G636570 PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 6.0e-116
Identity = 230/244 (94.26%), Postives = 230/244 (94.26%), Query Frame = 0


Query: 1   MSDSDSSPSPSPPPPPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60
           MSDSDSSPSPS    PPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG
Sbjct: 1   MSDSDSSPSPS----PPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60

Query: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHM 120
           HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHM
Sbjct: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHM 120

Query: 121 YHSDCIEKWLNVRGTCPVCRFVMPVDDRIKD---GGGGDEEEEEDM---EGGEGGEGRVV 180
           YHSDCIEKWLNVRGTCPVCRF MPVDDR KD   GGGGDEEEEEDM   EGGEGGEGRVV
Sbjct: 121 YHSDCIEKWLNVRGTCPVCRFAMPVDDRRKDGGGGGGGDEEEEEDMEGGEGGEGGEGRVV 180

Query: 181 VVNLWVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSST--QEQDPSSEDSSSFSFS 237
           VVNLWVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSST  QEQDPSSEDSSSFSFS
Sbjct: 181 VVNLWVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQEQDPSSEDSSSFSFS 240

[Close]

BLAST of Chy9G159640 vs. ExPASy TrEMBL
Match: A0A1S3CC03 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103498739 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.4e-109
Identity = 212/224 (94.64%), Postives = 213/224 (95.09%), Query Frame = 0


Query: 1   MSDSDSSPSPSPPPPPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60
           MSDSDSSPSPS    PPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG
Sbjct: 1   MSDSDSSPSPS----PPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60

Query: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHM 120
           HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELG DCAICLDGFEV EEAREMPCKHM
Sbjct: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGTDCAICLDGFEVAEEAREMPCKHM 120

Query: 121 YHSDCIEKWLNVRGTCPVCRFVMPVDDRIKDG--GGGDEEEEEDMEGGEGGEGRVVVVNL 180
           YHSDCIEKWLNVRGTCPVCRFVMPVDDR KDG  GGGD EE+EDMEGGE GEGRVVVVNL
Sbjct: 121 YHSDCIEKWLNVRGTCPVCRFVMPVDDRRKDGGVGGGDVEEDEDMEGGESGEGRVVVVNL 180

Query: 181 WVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPS 223
           WVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPS
Sbjct: 181 WVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPS 220

[Close]

BLAST of Chy9G159640 vs. ExPASy TrEMBL
Match: A0A6J1JYB9 (E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita maxima OX=3661 GN=LOC111489408 PE=4 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.4e-96
Identity = 198/239 (82.85%), Postives = 208/239 (87.03%), Query Frame = 0


Query: 1   MSDSDSSPSPSPPPPPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60
           MSDSDSS   S PPP  PPPR+LSMLLPFLLGLARTT GS+LNQIVVLDHAARTVVVFEG
Sbjct: 1   MSDSDSS---SSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEG 60

Query: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHM 120
           HVL+S LRDLSEKSGPLPASKASIDAMPRVG+TELGMDCAICLDGFEV  EAREMPCKHM
Sbjct: 61  HVLESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHM 120

Query: 121 YHSDCIEKWLNVRGTCPVCRFVMPVDDRIKDGGGGDEEEEEDMEGGEGGEGRVVVVNLWV 180
           YHSDCIEKWL VRGTCP+CRF MPVDD+      G ++++EDM   EG EGRVVVVNLWV
Sbjct: 121 YHSDCIEKWLRVRGTCPICRFTMPVDDQ-----EGKKDDDEDM---EGDEGRVVVVNLWV 180

Query: 181 NDGEPMD---VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN 237
           NDGEPMD   VESGL HGS DSDSES DEI VEVD SSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 NDGEPMDVESVESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

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BLAST of Chy9G159640 vs. ExPASy TrEMBL
Match: A0A6J1FLT0 (E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita moschata OX=3662 GN=LOC111445129 PE=4 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.7e-94
Identity = 195/239 (81.59%), Postives = 206/239 (86.19%), Query Frame = 0


Query: 1   MSDSDSSPSPSPPPPPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60
           MSDSDSS   S PPP  PPPR+LSMLLPFLLGLARTT GS+LNQIVVLDHAARTVVVFEG
Sbjct: 1   MSDSDSS---SSPPPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEG 60

Query: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHM 120
            VL+S LRDLSEKSGPLPASKASIDAMPRVG+TELGMDCAICLDGFEV  EAREMPCKH+
Sbjct: 61  RVLESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHL 120

Query: 121 YHSDCIEKWLNVRGTCPVCRFVMPVDDRIKDGGGGDEEEEEDMEGGEGGEGRVVVVNLWV 180
           YHSDCIEKWL VRGTCP+CRF MPVDD+      G ++++EDM   EG E RVVVVNLWV
Sbjct: 121 YHSDCIEKWLRVRGTCPICRFTMPVDDQ-----EGKKDDDEDM---EGDEDRVVVVNLWV 180

Query: 181 NDGEPMD---VESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN 237
           NDGEPMD   VESGL HGS DSDSES DEI VEVD SSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 NDGEPMDVESVESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

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BLAST of Chy9G159640 vs. NCBI nr
Match: KAA0039492.1 (E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK15247.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 428 bits (1100), Expect = 2.08e-150
Identity = 226/238 (94.96%), Postives = 227/238 (95.38%), Query Frame = 0


Query: 1   MSDSDSSPSPSPPPPPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60
           MSDSDSSPSPS    PPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG
Sbjct: 1   MSDSDSSPSPS----PPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60

Query: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHM 120
           HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELG DCAICLDGFEV EEAREMPCKHM
Sbjct: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGTDCAICLDGFEVAEEAREMPCKHM 120

Query: 121 YHSDCIEKWLNVRGTCPVCRFVMPVDDRIKDGG--GGDEEEEEDMEGGEGGEGRVVVVNL 180
           YHSDCIEKWLNVRGTCPVCRFVMPVDDR KDGG  GGD EE+EDMEGGE GEGRVVVVNL
Sbjct: 121 YHSDCIEKWLNVRGTCPVCRFVMPVDDRRKDGGVGGGDVEEDEDMEGGESGEGRVVVVNL 180

Query: 181 WVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN 236
           WVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 WVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN 234

[Close]

BLAST of Chy9G159640 vs. NCBI nr
Match: XP_011656279.1 (E3 ubiquitin-protein ligase MPSR1 [Cucumis sativus] >KGN52465.1 hypothetical protein Csa_008626 [Cucumis sativus])

HSP 1 Score: 427 bits (1098), Expect = 5.20e-150
Identity = 230/244 (94.26%), Postives = 230/244 (94.26%), Query Frame = 0


Query: 1   MSDSDSSPSPSPPPPPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60
           MSDSDSSPSPS    PPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG
Sbjct: 1   MSDSDSSPSPS----PPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60

Query: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHM 120
           HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHM
Sbjct: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHM 120

Query: 121 YHSDCIEKWLNVRGTCPVCRFVMPVDDRIKDGGGG---DEEEEEDMEGGEGGEG---RVV 180
           YHSDCIEKWLNVRGTCPVCRF MPVDDR KDGGGG   DEEEEEDMEGGEGGEG   RVV
Sbjct: 121 YHSDCIEKWLNVRGTCPVCRFAMPVDDRRKDGGGGGGGDEEEEEDMEGGEGGEGGEGRVV 180

Query: 181 VVNLWVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQ--DPSSEDSSSFSFS 236
           VVNLWVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQ  DPSSEDSSSFSFS
Sbjct: 181 VVNLWVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQEQDPSSEDSSSFSFS 240

[Close]

BLAST of Chy9G159640 vs. NCBI nr
Match: XP_008459699.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo])

HSP 1 Score: 405 bits (1040), Expect = 2.25e-141
Identity = 212/224 (94.64%), Postives = 213/224 (95.09%), Query Frame = 0


Query: 1   MSDSDSSPSPSPPPPPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60
           MSDSDSSPSPS    PPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG
Sbjct: 1   MSDSDSSPSPS----PPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60

Query: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHM 120
           HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELG DCAICLDGFEV EEAREMPCKHM
Sbjct: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGTDCAICLDGFEVAEEAREMPCKHM 120

Query: 121 YHSDCIEKWLNVRGTCPVCRFVMPVDDRIKDGG--GGDEEEEEDMEGGEGGEGRVVVVNL 180
           YHSDCIEKWLNVRGTCPVCRFVMPVDDR KDGG  GGD EE+EDMEGGE GEGRVVVVNL
Sbjct: 121 YHSDCIEKWLNVRGTCPVCRFVMPVDDRRKDGGVGGGDVEEDEDMEGGESGEGRVVVVNL 180

Query: 181 WVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPS 222
           WVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPS
Sbjct: 181 WVNDGEPMDVESGLEHGSEDSDSESQDEIMVEVDGSSTQEQDPS 220

[Close]

BLAST of Chy9G159640 vs. NCBI nr
Match: XP_022993365.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita maxima])

HSP 1 Score: 362 bits (930), Expect = 1.33e-124
Identity = 198/239 (82.85%), Postives = 208/239 (87.03%), Query Frame = 0


Query: 1   MSDSDSSPSPSPPPPPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60
           MSDSDSS SP   PP  PPPR+LSMLLPFLLGLARTT GS+LNQIVVLDHAARTVVVFEG
Sbjct: 1   MSDSDSSSSP---PPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEG 60

Query: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHM 120
           HVL+S LRDLSEKSGPLPASKASIDAMPRVG+TELGMDCAICLDGFEV  EAREMPCKHM
Sbjct: 61  HVLESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHM 120

Query: 121 YHSDCIEKWLNVRGTCPVCRFVMPVDDRIKDGGGGDEEEEEDMEGGEGGEGRVVVVNLWV 180
           YHSDCIEKWL VRGTCP+CRF MPVDD+      G ++++EDMEG EG   RVVVVNLWV
Sbjct: 121 YHSDCIEKWLRVRGTCPICRFTMPVDDQE-----GKKDDDEDMEGDEG---RVVVVNLWV 180

Query: 181 NDGEPMDVES---GLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN 236
           NDGEPMDVES   GL HGS DSDSES DEI VEVD SSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 NDGEPMDVESVESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

[Close]

BLAST of Chy9G159640 vs. NCBI nr
Match: XP_023551536.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 361 bits (927), Expect = 3.82e-124
Identity = 197/239 (82.43%), Postives = 208/239 (87.03%), Query Frame = 0


Query: 1   MSDSDSSPSPSPPPPPPPPPRTLSMLLPFLLGLARTTTGSNLNQIVVLDHAARTVVVFEG 60
           MSDSDSS SP   PP  PPPR+LSMLLPFLLGLARTT GS+LNQIVVLDHAARTVVVFEG
Sbjct: 1   MSDSDSSSSP---PPSLPPPRSLSMLLPFLLGLARTTPGSHLNQIVVLDHAARTVVVFEG 60

Query: 61  HVLDSLLRDLSEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHM 120
           HVL+S LRDLSEKSGPLPASKASIDAMPRVG+TELGMDCAICLDGFEV  EAREMPCKH+
Sbjct: 61  HVLESFLRDLSEKSGPLPASKASIDAMPRVGITELGMDCAICLDGFEVGAEAREMPCKHL 120

Query: 121 YHSDCIEKWLNVRGTCPVCRFVMPVDDRIKDGGGGDEEEEEDMEGGEGGEGRVVVVNLWV 180
           YHSDCIEKWL VRGTCP+CRF MPVDD+      G ++++EDMEG EG   RVVVVNLWV
Sbjct: 121 YHSDCIEKWLRVRGTCPICRFTMPVDDQE-----GKKDDDEDMEGDEG---RVVVVNLWV 180

Query: 181 NDGEPMDVES---GLEHGSEDSDSESQDEIMVEVDGSSTQEQDPSSEDSSSFSFSSFFN 236
           NDGEPMDVES   GL HGS DSDSES DEI VEVD SSTQEQDPSSEDSSSFSFSSFFN
Sbjct: 181 NDGEPMDVESVESGLGHGSGDSDSESHDEITVEVDASSTQEQDPSSEDSSSFSFSSFFN 228

[Close]

BLAST of Chy9G159640 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 112.5 bits (280), Expect = 4.8e-25
Identity = 71/189 (37.57%), Postives = 102/189 (53.97%), Query Frame = 0


Query: 21  RTLSMLLPFLLGLARTTT--------GSNLNQIVVLDHAARTVVVFEGHV-LDSLLRDL- 80
           R L + LPFLLG +   +         S+  +I++++   + ++V EG   ++ LLR L 
Sbjct: 24  RDLYLFLPFLLGFSDQESSNGDDDDVASSRERIILVNPFTQGMIVLEGSSGMNPLLRSLL 83

Query: 81  -SEKSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAREMPCKHMYHSDCIEKW 140
            S + G  PASKASIDAMP V +     +C ICL+ ++ +E  +EMPCKH +H  CIEKW
Sbjct: 84  ESREEGRPPASKASIDAMPIVEIDGCEGECVICLEEWKSEETVKEMPCKHRFHGGCIEKW 143

Query: 141 LNVRGTCPVCRFVMPVDDRIKDGGGGDEEEEEDMEGGEGGEGRVVVVNLWVNDGEPMDVE 199
           L   G+CPVCR+ MPVD        GDE       G +  +G  + V    NDG  +   
Sbjct: 144 LGFHGSCPVCRYEMPVD--------GDE------IGKKRNDGNEIWVRFSFNDGRRIRDF 198

[Close]

BLAST of Chy9G159640 vs. TAIR 10
Match: AT4G26400.2 (RING/U-box superfamily protein )

HSP 1 Score: 97.1 bits (240), Expect = 2.1e-20
Identity = 44/97 (45.36%), Postives = 60/97 (61.86%), Query Frame = 0


Query: 58  FEGHVLDSLLRDLSE----KSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAR 117
           F G  LD LL  L++    + G LPA K  +D +P V ++E  + C+ICLD F+   EA+
Sbjct: 197 FVGSSLDHLLEHLADNDSIRHGSLPARKEVVDNLPTVKISE-SLQCSICLDDFDKGSEAK 256

Query: 118 EMPCKHMYHSDCIEKWLNVRGTCPVCRFVMPVDDRIK 151
           EMPCKH +H  CI  WL +  +CPVCR+ +P DD  K
Sbjct: 257 EMPCKHKFHIRCIVPWLELHSSCPVCRYELPPDDETK 292

[Close]

BLAST of Chy9G159640 vs. TAIR 10
Match: AT4G26400.1 (RING/U-box superfamily protein )

HSP 1 Score: 97.1 bits (240), Expect = 2.1e-20
Identity = 44/97 (45.36%), Postives = 60/97 (61.86%), Query Frame = 0


Query: 58  FEGHVLDSLLRDLSE----KSGPLPASKASIDAMPRVGVTELGMDCAICLDGFEVDEEAR 117
           F G  LD LL  L++    + G LPA K  +D +P V ++E  + C+ICLD F+   EA+
Sbjct: 197 FVGSSLDHLLEHLADNDSIRHGSLPARKEVVDNLPTVKISE-SLQCSICLDDFDKGSEAK 256

Query: 118 EMPCKHMYHSDCIEKWLNVRGTCPVCRFVMPVDDRIK 151
           EMPCKH +H  CI  WL +  +CPVCR+ +P DD  K
Sbjct: 257 EMPCKHKFHIRCIVPWLELHSSCPVCRYELPPDDETK 292

[Close]

BLAST of Chy9G159640 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 95.5 bits (236), Expect = 6.1e-20
Identity = 44/103 (42.72%), Postives = 64/103 (62.14%), Query Frame = 0


Query: 57  VFEGHVLDSLLRDLSE---------KSGPLPASKASIDAMPRVGV----TELGMDCAICL 116
           +  G   + LL  LS+         +SG  PASK++I+++PRV +    T+   +CA+C 
Sbjct: 160 ILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDCHTKAEANCAVCT 219

Query: 117 DGFEVDEEAREMPCKHMYHSDCIEKWLNVRGTCPVCRFVMPVD 147
           + FE   E REMPCKH++H DCI  WL++R +CPVCRF +P D
Sbjct: 220 EVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELPSD 262

[Close]

BLAST of Chy9G159640 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 94.0 bits (232), Expect = 1.8e-19
Identity = 53/146 (36.30%), Postives = 82/146 (56.16%), Query Frame = 0


Query: 57  VFEGHVLDSLLRDLSE---------KSGPLPASKASIDAMPRVGVTELGM----DCAICL 116
           +  G   + LL  LS+         +SG  PASK++I+++PRV +++  +    +CA+C 
Sbjct: 144 ILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCHIGSEANCAVCT 203

Query: 117 DGFEVDEEAREMPCKHMYHSDCIEKWLNVRGTCPVCRFVMPVDDRIKDGGGGDEEEEEDM 176
           + FE + EAREMPCKH++H DCI  WL++R +CPVCRF +P +   +     +  EE++ 
Sbjct: 204 EIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRR----SNNNEEDNA 263

Query: 177 EGG-----EGGEGRVVVVNLWVNDGE 185
            G       GG   V   N  + DGE
Sbjct: 264 VGMTIWRLPGGGFAVGRFNAAMRDGE 285

The following BLAST results are available for this feature:

BLAST of Chy9G159640 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LQX2	6.7e-24	37.57	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
Q9SNB6	8.5e-19	42.72	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
P0CH30	1.5e-18	37.88	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q940T5	2.5e-18	36.30	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q8LPN7	9.4e-18	38.40	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]

BLAST of Chy9G159640 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T7P7	3.5e-116	94.96	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0KS87	6.0e-116	94.26	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G636570 P...	[more]
A0A1S3CC03	2.4e-109	94.64	E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103498739 P...	[more]
A0A6J1JYB9	1.4e-96	82.85	E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita maxima OX=3661 GN=LOC111489...	[more]
A0A6J1FLT0	1.7e-94	81.59	E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of Chy9G159640 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0039492.1	2.08e-150	94.96	E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK15247.1 E3...	[more]
XP_011656279.1	5.20e-150	94.26	E3 ubiquitin-protein ligase MPSR1 [Cucumis sativus] >KGN52465.1 hypothetical pro...	[more]
XP_008459699.1	2.25e-141	94.64	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo]	[more]
XP_022993365.1	1.33e-124	82.85	E3 ubiquitin-protein ligase RNF181-like [Cucurbita maxima]	[more]
XP_023551536.1	3.82e-124	82.43	E3 ubiquitin-protein ligase RNF181-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Chy9G159640 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26800.1	4.8e-25	37.57	RING/U-box superfamily protein	[more]
AT4G26400.2	2.1e-20	45.36	RING/U-box superfamily protein	[more]
AT4G26400.1	2.1e-20	45.36	RING/U-box superfamily protein	[more]
AT3G46620.1	6.1e-20	42.72	zinc finger (C3HC4-type RING finger) family protein	[more]
AT5G59550.1	1.8e-19	36.30	zinc finger (C3HC4-type RING finger) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 98..140 e-value: 0.002 score: 13.2
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 99..139 e-value: 1.0E-8 score: 45.0
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 98..140 e-value: 4.8E-14 score: 52.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 99..140 score: 12.563043
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 67..145 e-value: 7.4E-21 score: 75.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 7..21
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 147..171
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 184..236
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 208..236
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 44..150
None	No IPR available	PANTHER	PTHR15710:SF132	E3 UBIQUITIN-PROTEIN LIGASE MPSR1	coord: 44..150
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 98..146

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy9G159640.1	Chy9G159640.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding