Chy9G157410 (gene) Cucumber (hystrix) v1

Overview


Name	Chy9G157410
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	E3 ubiquitin-protein ligase
Location	chrH09: 262655 .. 263325 (+)
RNA-Seq Expression	Chy9G157410
Synteny	Chy9G157410

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGATGATGATAAAACCACAACTAATTGCAATATCTGTACAGACGACAAAGCCTTGGTGAACATCTTCACAAACCACGAGTGCTCCCATTCCTTCTGCAAAGATTGCATCTCTAATCATATCACAGCCAAATTGGAAGATAACATAGCCAACGTCAAGTGTCCACAGCCAGGCTGCGAGGCATTGCTACACCCGGATGTCTGTCATTCCTTCGTCCCCCAAAACGTACTTGATCGCTGGGGAGATGTGTTGTGCGAGGCCTTCATTCTAGGGAAACATAGACTGATCTATTGCCCCTTCATGGACTGCTCGGTGGCGCTTATCGATGACGGAGACGAGGCAACGAAGGAAGCAGAGTGTCCGAGCTGTAACAGAATGTTCTGCGCCAAGTGTAATGTGGTGTGGCATGGGGGGGGGTAGAATGCGAGGAGTTTCAAAAGCTGTGTTTGGAGGAAAAGGAAAGAGATGATCATTTGTTGGCAATAAAGTTGGCGGAGCAGGAGAATTGGAAGCGATGCCCACATTGTAGGACTTGCGTGGAGATGATTGAGGGTTGCCCTTATATAATTTGTAGGTGTGGAACTAAATTTTGCTATAGTTGTGGAGCTAAGTGGGGTGGCAGTCATGCTTGTCCCGAATCTGCTGAGGCACGGAAGTACCATAAATAA

mRNA sequence

ATGGCAGATGATGATAAAACCACAACTAATTGCAATATCTGTACAGACGACAAAGCCTTGGTGAACATCTTCACAAACCACGAGTGCTCCCATTCCTTCTGCAAAGATTGCATCTCTAATCATATCACAGCCAAATTGGAAGATAACATAGCCAACGTCAAGTGTCCACAGCCAGGCTGCGAGGCATTGCTACACCCGGATGTCTGTCATTCCTTCGTCCCCCAAAACGTACTTGATCGCTGGGGAGATGTGTTGTGCGAGGCCTTCATTCTAGGGAAACATAGACTGATCTATTGCCCCTTCATGGACTGCTCGGTGGCGCTTATCGATGACGGAGACGAGGCAACGAAGGAAGCAGAGTGTCCGAGCTGTAACAGAATGTTCTGCGCCAAGTGTAATGTGGTGTGGCATGGGGGGGGGTGTGGAACTAAATTTTGCTATAGTTGTGGAGCTAAGTGGGGTGGCAGTCATGCTTGTCCCGAATCTGCTGAGGCACGGAAGTACCATAAATAA

Coding sequence (CDS)

ATGGCAGATGATGATAAAACCACAACTAATTGCAATATCTGTACAGACGACAAAGCCTTGGTGAACATCTTCACAAACCACGAGTGCTCCCATTCCTTCTGCAAAGATTGCATCTCTAATCATATCACAGCCAAATTGGAAGATAACATAGCCAACGTCAAGTGTCCACAGCCAGGCTGCGAGGCATTGCTACACCCGGATGTCTGTCATTCCTTCGTCCCCCAAAACGTACTTGATCGCTGGGGAGATGTGTTGTGCGAGGCCTTCATTCTAGGGAAACATAGACTGATCTATTGCCCCTTCATGGACTGCTCGGTGGCGCTTATCGATGACGGAGACGAGGCAACGAAGGAAGCAGAGTGTCCGAGCTGTAACAGAATGTTCTGCGCCAAGTGTAATGTGGTGTGGCATGGGGGGGGGTGTGGAACTAAATTTTGCTATAGTTGTGGAGCTAAGTGGGGTGGCAGTCATGCTTGTCCCGAATCTGCTGAGGCACGGAAGTACCATAAATAA

Protein sequence

MADDDKTTTNCNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLEDNIANVKCPQPGCEALLHPDVCHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAECPSCNRMFCAKCNVVWHGGGCGTKFCYSCGAKWGGSHACPESAEARKYHK*

Homology

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BLAST of Chy9G157410 vs. ExPASy Swiss-Prot
Match: Q9LVX0 (Probable E3 ubiquitin-protein ligase ARI3 OS=Arabidopsis thaliana OX=3702 GN=ARI3 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 6.6e-13
Identity = 42/145 (28.97%), Postives = 62/145 (42.76%), Query Frame = 0


Query: 6   KTTTNCNICTDDKALVNIFTNHECSHSFCKDCISNHITAKL-EDNIANVKCPQPGCEALL 65
           K T  C++C +D    N+ T  EC H FC DC   H T K+ E     + C    C+A+ 
Sbjct: 116 KKTMKCDVCMEDDLPSNVMTRMECGHRFCNDCWIGHFTVKINEGESKRILCMAHECKAIC 175

Query: 66  HPDVCHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAECPSC 125
             DV    V   + DR+   L E+++   + + +CP                 +  C S 
Sbjct: 176 DEDVVRKLVSPELADRYDRFLIESYVEDNNMVKWCP----------------SKPHCGSA 235

Query: 126 NRMFCAKCNVVWHGGGCGTKFCYSC 150
            R      +VV  G  CG +FC+SC
Sbjct: 236 IRKIEDGHDVVEVGCSCGLQFCFSC 244

[Close]

BLAST of Chy9G157410 vs. ExPASy Swiss-Prot
Match: Q9SKC4 (Probable E3 ubiquitin-protein ligase ARI10 OS=Arabidopsis thaliana OX=3702 GN=ARI10 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.6e-09
Identity = 47/212 (22.17%), Postives = 77/212 (36.32%), Query Frame = 0


Query: 11  CNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLED--NIANVKCPQPGCEALLHPDV 70
           C IC +      I  +  C H +CK C + +IT K+ED      VKCP+P C A++  D+
Sbjct: 121 CGICFESYTRKEI-ASVSCGHPYCKTCWTGYITTKIEDGPGCLRVKCPEPSCYAVVGQDM 180

Query: 71  CHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAECPSCNRMF 130
                 +   D++      +++    ++ +CP   C  A ++ G+ +  +  C  C+  F
Sbjct: 181 IDEVTEKKDKDKYYRYFLRSYVEDGKKMKWCPSPGCECA-VEFGESSGYDVAC-LCSYRF 240

Query: 131 CAKC-------------------------NVVW------------------HG------- 171
           C  C                         N  W                  HG       
Sbjct: 241 CWNCSEDAHSPVDCETVSKWIFKNQDESENKNWILANSKPCPKCKRPIEKSHGCNHMTCS 300

[Close]

BLAST of Chy9G157410 vs. ExPASy Swiss-Prot
Match: A2VEA3 (E3 ubiquitin-protein ligase ARIH1 OS=Bos taurus OX=9913 GN=ARIH1 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 5.8e-09
Identity = 35/117 (29.91%), Postives = 51/117 (43.59%), Query Frame = 0


Query: 24  FTNHECSHSFCKDCISNHITAKL--EDNIANVKCPQPGCEALLHPD-VCHSFVPQNVLDR 83
           FT  EC H FC  C S ++T K+  E     + CP  GC+ L+  + V        V  +
Sbjct: 196 FTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLK 255

Query: 84  WGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAECPSCNRMFCAKCNVVWH 138
           +  ++  +F+     L +CP  DC   +     +A K   C  C R FC  C   WH
Sbjct: 256 YQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDA-KPVRC-KCGRQFCFNCGENWH 310

[Close]

BLAST of Chy9G157410 vs. ExPASy Swiss-Prot
Match: Q9Y4X5 (E3 ubiquitin-protein ligase ARIH1 OS=Homo sapiens OX=9606 GN=ARIH1 PE=1 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 5.8e-09
Identity = 35/117 (29.91%), Postives = 51/117 (43.59%), Query Frame = 0


Query: 24  FTNHECSHSFCKDCISNHITAKL--EDNIANVKCPQPGCEALLHPD-VCHSFVPQNVLDR 83
           FT  EC H FC  C S ++T K+  E     + CP  GC+ L+  + V        V  +
Sbjct: 198 FTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLK 257

Query: 84  WGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAECPSCNRMFCAKCNVVWH 138
           +  ++  +F+     L +CP  DC   +     +A K   C  C R FC  C   WH
Sbjct: 258 YQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDA-KPVRC-KCGRQFCFNCGENWH 312

[Close]

BLAST of Chy9G157410 vs. ExPASy Swiss-Prot
Match: Q9Z1K5 (E3 ubiquitin-protein ligase ARIH1 OS=Mus musculus OX=10090 GN=Arih1 PE=1 SV=3)

HSP 1 Score: 62.4 bits (150), Expect = 5.8e-09
Identity = 35/117 (29.91%), Postives = 51/117 (43.59%), Query Frame = 0


Query: 24  FTNHECSHSFCKDCISNHITAKL--EDNIANVKCPQPGCEALLHPD-VCHSFVPQNVLDR 83
           FT  EC H FC  C S ++T K+  E     + CP  GC+ L+  + V        V  +
Sbjct: 196 FTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLK 255

Query: 84  WGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAECPSCNRMFCAKCNVVWH 138
           +  ++  +F+     L +CP  DC   +     +A K   C  C R FC  C   WH
Sbjct: 256 YQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDA-KPVRC-KCGRQFCFNCGENWH 310

[Close]

BLAST of Chy9G157410 vs. ExPASy TrEMBL
Match: A0A0A0KSV5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G650600 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.5e-89
Identity = 161/222 (72.52%), Postives = 164/222 (73.87%), Query Frame = 0


Query: 1   MADDDKTTTNCNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLEDNIANVKCPQPGC 60
           MADDDKTTTNCNICTDDKALVN+FTNHECSHSFCKDCISNHI AKLEDNIANVKCPQPGC
Sbjct: 1   MADDDKTTTNCNICTDDKALVNMFTNHECSHSFCKDCISNHIAAKLEDNIANVKCPQPGC 60

Query: 61  EALLHPDVCHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAE 120
           EA+LHPDVCHSFVP+NVLDRWG VLCEAFILG HRLIYCPFMDCSVALIDDGDEATKEAE
Sbjct: 61  EAVLHPDVCHSFVPKNVLDRWGYVLCEAFILGNHRLIYCPFMDCSVALIDDGDEATKEAE 120

Query: 121 CPSCNRMFCAKCNVVWHGG----------------------------------------- 171
           CP CNRMFCAKCNVVWHGG                                         
Sbjct: 121 CPGCNRMFCAKCNVVWHGGVECEEFQKLCLEEKERDDHLLAIKLAEQENWKRCPHCRTYV 180

[Close]

BLAST of Chy9G157410 vs. ExPASy TrEMBL
Match: A0A5A7TCD1 (Putative E3 ubiquitin-protein ligase RNF217 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00360 PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.4e-82
Identity = 147/221 (66.52%), Postives = 155/221 (70.14%), Query Frame = 0


Query: 1   MADDDKTTTNCNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLEDNIANVKCPQPGC 60
           MADDDKTTT CNICTDDKA +N+FTNHECSHSFCKDCIS+HI AKLEDNIANVKCPQ  C
Sbjct: 1   MADDDKTTTTCNICTDDKASMNMFTNHECSHSFCKDCISSHIAAKLEDNIANVKCPQTDC 60

Query: 61  EALLHPDVCHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAE 120
           +ALLHPD+CHSF+P+N+ DRWGDVLCEAFILG HRLIYCPFMDCSVALIDDGDE  KEAE
Sbjct: 61  QALLHPDICHSFIPKNLFDRWGDVLCEAFILGNHRLIYCPFMDCSVALIDDGDEPAKEAE 120

Query: 121 CPSCNRMFCAKCNVVWHGG----------------------------------------- 171
           C  CNRMFCAKCNV WH G                                         
Sbjct: 121 CTRCNRMFCAKCNVKWHAGVECEEFQKLCLEENGRDDLLAMKLAEQQNWKRCPHCRIYVE 180

[Close]

BLAST of Chy9G157410 vs. ExPASy TrEMBL
Match: A0A6J1GX07 (probable E3 ubiquitin-protein ligase ARI10 OS=Cucurbita moschata OX=3662 GN=LOC111457924 PE=3 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 4.4e-44
Identity = 95/216 (43.98%), Postives = 121/216 (56.02%), Query Frame = 0


Query: 7   TTTNCNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLEDNIANVKCPQPGCEALLHP 66
           +TT C ICTD K+   +FT+  CSH+FC DCIS HI AKLED++A VKCP+P C A+L P
Sbjct: 70  STTLCTICTDTKSASEMFTSTGCSHTFCADCISKHIAAKLEDSMA-VKCPEPKCAAVLEP 129

Query: 67  DVCHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAECPSCNR 126
           ++C SFVP+ VL+RWGD L +A +L K +++YCPF DCS  ++D+ D   +  ECP C R
Sbjct: 130 EMCDSFVPKEVLERWGDALLKAMLL-KWKMVYCPFKDCSAGMVDEED--MEAVECPKCWR 189

Query: 127 MFCAKCNVVWHG-------------GG--------------------------------- 171
           MFCAKC V WHG             GG                                 
Sbjct: 190 MFCAKCGVGWHGDMNCTEFQRLLKNGGVDREEEMTMKLADERKWKRCPHCKILVEKIEGC 249

[Close]

BLAST of Chy9G157410 vs. ExPASy TrEMBL
Match: A0A6J1DGR7 (E3 ubiquitin-protein ligase RNF144A-like OS=Momordica charantia OX=3673 GN=LOC111020341 PE=3 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 9.8e-44
Identity = 87/133 (65.41%), Postives = 100/133 (75.19%), Query Frame = 0


Query: 8   TTNCNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLEDNIANVKCPQPGCEALLHPD 67
           T+ C ICTD K+   +FT+  CSHSFC DCIS HI AKLEDNIA VKCP+P CE+ L P 
Sbjct: 84  TSLCIICTDTKSAAEMFTSSACSHSFCADCISKHIAAKLEDNIA-VKCPEPKCESTLEPW 143

Query: 68  VCHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEA--TKEAECPSCN 127
           +C SFVP+ VL+RWGD LCEA ILG  R+ YCPF+DCS A+IDDG EA     AECPSC 
Sbjct: 144 MCDSFVPREVLERWGDALCEAIILGIPRM-YCPFVDCSAAMIDDGGEAATVTAAECPSCR 203

Query: 128 RMFCAKCNVVWHG 139
           RMFCA+C V WHG
Sbjct: 204 RMFCAQCKVGWHG 214

[Close]

BLAST of Chy9G157410 vs. ExPASy TrEMBL
Match: A0A6J1IU95 (probable E3 ubiquitin-protein ligase ARI5 OS=Cucurbita maxima OX=3661 GN=LOC111479983 PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 2.0e-41
Identity = 91/214 (42.52%), Postives = 116/214 (54.21%), Query Frame = 0


Query: 9   TNCNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLEDNIANVKCPQPGCEALLHPDV 68
           T C ICTD K+   +FT+  CSH+FC DCIS HI AKL D++A VKCP+P C A+L P++
Sbjct: 71  TLCTICTDTKSASEMFTSTGCSHTFCADCISKHIAAKLVDSLA-VKCPEPKCAAVLKPEM 130

Query: 69  CHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAECPSCNRMF 128
           C SFVP+ VL+RWGD L +A +L K +++YCPF DCS  ++D+ D   +  ECP C RMF
Sbjct: 131 CDSFVPKEVLERWGDALLKAMLL-KWKMVYCPFKDCSAGMVDEEDR--EAVECPKCWRMF 190

Query: 129 CAKCNVVWH-------------GGG----------------------------------- 171
           CAKC V WH              GG                                   
Sbjct: 191 CAKCGVGWHADMNCTEFQRLLKNGGVDREEEMTMKLADERKWKRCPHCKILVEKILGCLH 250

[Close]

BLAST of Chy9G157410 vs. NCBI nr
Match: XP_004141646.1 (probable E3 ubiquitin-protein ligase ARI3 [Cucumis sativus])

HSP 1 Score: 334 bits (856), Expect = 1.43e-114
Identity = 161/222 (72.52%), Postives = 164/222 (73.87%), Query Frame = 0


Query: 1   MADDDKTTTNCNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLEDNIANVKCPQPGC 60
           MADDDKTTTNCNICTDDKALVN+FTNHECSHSFCKDCISNHI AKLEDNIANVKCPQPGC
Sbjct: 1   MADDDKTTTNCNICTDDKALVNMFTNHECSHSFCKDCISNHIAAKLEDNIANVKCPQPGC 60

Query: 61  EALLHPDVCHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAE 120
           EA+LHPDVCHSFVP+NVLDRWG VLCEAFILG HRLIYCPFMDCSVALIDDGDEATKEAE
Sbjct: 61  EAVLHPDVCHSFVPKNVLDRWGYVLCEAFILGNHRLIYCPFMDCSVALIDDGDEATKEAE 120

Query: 121 CPSCNRMFCAKCNVVWHGG----------------------------------------- 170
           CP CNRMFCAKCNVVWHGG                                         
Sbjct: 121 CPGCNRMFCAKCNVVWHGGVECEEFQKLCLEEKERDDHLLAIKLAEQENWKRCPHCRTYV 180

[Close]

BLAST of Chy9G157410 vs. NCBI nr
Match: KAE8649011.1 (hypothetical protein Csa_009107 [Cucumis sativus])

HSP 1 Score: 324 bits (831), Expect = 1.03e-109
Identity = 157/218 (72.02%), Postives = 160/218 (73.39%), Query Frame = 0


Query: 1   MADDDKTTTNCNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLEDNIANVKCPQPGC 60
           MADDDKTTTNCNICTDDKALVN+FTNHECSHSFCKDCISNHI AKLEDNIANVKCPQPGC
Sbjct: 12  MADDDKTTTNCNICTDDKALVNMFTNHECSHSFCKDCISNHIAAKLEDNIANVKCPQPGC 71

Query: 61  EALLHPDVCHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAE 120
           EA+LHPDVCHSFVP+NVLDRWG VLCEAFILG HRLIYCPFMDCSVALIDDGDEATKEAE
Sbjct: 72  EAVLHPDVCHSFVPKNVLDRWGYVLCEAFILGNHRLIYCPFMDCSVALIDDGDEATKEAE 131

Query: 121 CPSCNRMFCAKCNVVWHGG----------------------------------------- 166
           CP CNRMFCAKCNVVWHGG                                         
Sbjct: 132 CPGCNRMFCAKCNVVWHGGVECEEFQKLCLEEKERDDHLLAIKLAEQENWKRCPHCRTYV 191

[Close]

BLAST of Chy9G157410 vs. NCBI nr
Match: KAA0039247.1 (putative E3 ubiquitin-protein ligase RNF217 isoform X2 [Cucumis melo var. makuwa] >TYK00433.1 putative E3 ubiquitin-protein ligase RNF217 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 310 bits (794), Expect = 3.86e-105
Identity = 147/221 (66.52%), Postives = 155/221 (70.14%), Query Frame = 0


Query: 1   MADDDKTTTNCNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLEDNIANVKCPQPGC 60
           MADDDKTTT CNICTDDKA +N+FTNHECSHSFCKDCIS+HI AKLEDNIANVKCPQ  C
Sbjct: 1   MADDDKTTTTCNICTDDKASMNMFTNHECSHSFCKDCISSHIAAKLEDNIANVKCPQTDC 60

Query: 61  EALLHPDVCHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAE 120
           +ALLHPD+CHSF+P+N+ DRWGDVLCEAFILG HRLIYCPFMDCSVALIDDGDE  KEAE
Sbjct: 61  QALLHPDICHSFIPKNLFDRWGDVLCEAFILGNHRLIYCPFMDCSVALIDDGDEPAKEAE 120

Query: 121 CPSCNRMFCAKCNVVWHGG----------------------------------------- 170
           C  CNRMFCAKCNV WH G                                         
Sbjct: 121 CTRCNRMFCAKCNVKWHAGVECEEFQKLCLEENGRDDLLAMKLAEQQNWKRCPHCRIYVE 180

[Close]

BLAST of Chy9G157410 vs. NCBI nr
Match: XP_022956145.1 (probable E3 ubiquitin-protein ligase ARI10 [Cucurbita moschata])

HSP 1 Score: 183 bits (464), Expect = 3.42e-54
Identity = 95/215 (44.19%), Postives = 120/215 (55.81%), Query Frame = 0


Query: 8   TTNCNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLEDNIANVKCPQPGCEALLHPD 67
           TT C ICTD K+   +FT+  CSH+FC DCIS HI AKLED++A VKCP+P C A+L P+
Sbjct: 71  TTLCTICTDTKSASEMFTSTGCSHTFCADCISKHIAAKLEDSMA-VKCPEPKCAAVLEPE 130

Query: 68  VCHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAECPSCNRM 127
           +C SFVP+ VL+RWGD L +A +L K +++YCPF DCS  ++D+ D   +  ECP C RM
Sbjct: 131 MCDSFVPKEVLERWGDALLKAMLL-KWKMVYCPFKDCSAGMVDEED--MEAVECPKCWRM 190

Query: 128 FCAKCNVVWHG-------------GG---------------------------------- 170
           FCAKC V WHG             GG                                  
Sbjct: 191 FCAKCGVGWHGDMNCTEFQRLLKNGGVDREEEMTMKLADERKWKRCPHCKILVEKIEGCL 250

[Close]

BLAST of Chy9G157410 vs. NCBI nr
Match: XP_022152679.1 (E3 ubiquitin-protein ligase RNF144A-like [Momordica charantia])

HSP 1 Score: 183 bits (465), Expect = 4.74e-54
Identity = 86/130 (66.15%), Postives = 98/130 (75.38%), Query Frame = 0


Query: 11  CNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLEDNIANVKCPQPGCEALLHPDVCH 70
           C ICTD K+   +FT+  CSHSFC DCIS HI AKLEDNIA VKCP+P CE+ L P +C 
Sbjct: 87  CIICTDTKSAAEMFTSSACSHSFCADCISKHIAAKLEDNIA-VKCPEPKCESTLEPWMCD 146

Query: 71  SFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATK--EAECPSCNRMF 130
           SFVP+ VL+RWGD LCEA ILG  R+ YCPF+DCS A+IDDG EA     AECPSC RMF
Sbjct: 147 SFVPREVLERWGDALCEAIILGIPRM-YCPFVDCSAAMIDDGGEAATVTAAECPSCRRMF 206

Query: 131 CAKCNVVWHG 138
           CA+C V WHG
Sbjct: 207 CAQCKVGWHG 214

[Close]

BLAST of Chy9G157410 vs. TAIR 10
Match: AT3G53690.1 (RING/U-box superfamily protein )

HSP 1 Score: 146.0 bits (367), Expect = 2.8e-35
Identity = 59/138 (42.75%), Postives = 87/138 (63.04%), Query Frame = 0


Query: 2   ADDDKTTT-NCNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLEDNIANVKCPQPGC 61
           +   KT T +C IC D K+++  F    CSH +C DC+S +I AKL+DNI +++CP  GC
Sbjct: 105 SSSSKTATFDCEICVDSKSIIESFRIGGCSHFYCNDCVSKYIAAKLQDNILSIECPVSGC 164

Query: 62  EALLHPDVCHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCS-VALIDDGDEATKEA 121
              L PD C   +P+ V DRWGD LCEA ++ + +  YCP+ DCS +  +++ +   K++
Sbjct: 165 SGRLEPDQCRQILPKEVFDRWGDALCEAVVM-RSKKFYCPYKDCSALVFLEESEVKMKDS 224

Query: 122 ECPSCNRMFCAKCNVVWH 138
           ECP C+RM C +C   WH
Sbjct: 225 ECPHCHRMVCVECGTQWH 241

[Close]

BLAST of Chy9G157410 vs. TAIR 10
Match: AT3G14250.1 (RING/U-box superfamily protein )

HSP 1 Score: 132.1 bits (331), Expect = 4.2e-31
Identity = 63/206 (30.58%), Postives = 92/206 (44.66%), Query Frame = 0


Query: 11  CNICTDDKALVNIFT-NHECSHSFCKDCISNHITAKLEDNIANVKCPQPGCEALLHPDVC 70
           C IC D+K   +IF     C+H++C DC   ++  K+++N + +KCP   C  L+ P  C
Sbjct: 95  CMICMDEKPSSDIFRGTTNCTHAYCTDCTVRYVATKIKENASRIKCPDVECTRLIEPYTC 154

Query: 71  HSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALI--DDGDEATKEAECPSCNRM 130
              +P++V DRW  +LCE+ I    +  YCPF DCS  ++  ++GD    + EC SC+R+
Sbjct: 155 RDLIPKDVFDRWEKILCESLISSWDK-FYCPFKDCSAMMVNNENGDANVTQTECRSCHRL 214

Query: 131 FCAKCNVVWHGG------------------------------------------------ 160
           FC +C V WH G                                                
Sbjct: 215 FCVQCKVTWHAGIGCDEFQRFGNTKKKSSDEDDALLIQMAKNKQWRRCPSCKFYVDKVEG 274

[Close]

BLAST of Chy9G157410 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 76.3 bits (186), Expect = 2.7e-14
Identity = 44/146 (30.14%), Postives = 62/146 (42.47%), Query Frame = 0


Query: 11  CNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLEDNIANVKCPQPGCEALLHPDVCH 70
           C IC +D     +F+  +C H FC  C+  H+  KL   +A  KCP  GC++ L  D C 
Sbjct: 301 CAICFNDIVAEGMFSVDKCRHRFCFQCVKQHVEVKLLHGMA-PKCPHDGCKSELVIDACG 360

Query: 71  SFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDG-DEATKE----------A 130
             +   +   W   L E  I    R +YCP+  CS  +      E+ K            
Sbjct: 361 KLLTPKLSKLWQQRLQENAIPVTER-VYCPYPRCSALMSKTKISESAKSLLSLYPKSGVR 420

Query: 131 ECPSCNRMFCAKCNVVWHGGGCGTKF 146
            C  C  +FC  C V WHG    T++
Sbjct: 421 RCVECRGLFCVDCKVPWHGNLSCTEY 444

[Close]

BLAST of Chy9G157410 vs. TAIR 10
Match: AT3G27710.1 (RING/U-box superfamily protein )

HSP 1 Score: 75.5 bits (184), Expect = 4.7e-14
Identity = 42/145 (28.97%), Postives = 62/145 (42.76%), Query Frame = 0


Query: 6   KTTTNCNICTDDKALVNIFTNHECSHSFCKDCISNHITAKL-EDNIANVKCPQPGCEALL 65
           K T  C++C +D    N+ T  EC H FC DC   H T K+ E     + C    C+A+ 
Sbjct: 116 KKTMKCDVCMEDDLPSNVMTRMECGHRFCNDCWIGHFTVKINEGESKRILCMAHECKAIC 175

Query: 66  HPDVCHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAECPSC 125
             DV    V   + DR+   L E+++   + + +CP                 +  C S 
Sbjct: 176 DEDVVRKLVSPELADRYDRFLIESYVEDNNMVKWCP----------------SKPHCGSA 235

Query: 126 NRMFCAKCNVVWHGGGCGTKFCYSC 150
            R      +VV  G  CG +FC+SC
Sbjct: 236 IRKIEDGHDVVEVGCSCGLQFCFSC 244

[Close]

BLAST of Chy9G157410 vs. TAIR 10
Match: AT5G37560.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 4.0e-13
Identity = 51/212 (24.06%), Postives = 77/212 (36.32%), Query Frame = 0


Query: 2   ADDDKTTTNCNICTDDKALVNIFTNHECSHSFCKDCISNHITAKLEDNIANVKCPQPGCE 61
           A  +K  T C+IC+D     ++  N +C H  C  C+   +  KL   I    C + GC+
Sbjct: 173 ATQEKPLT-CSICSDKTDAEHMLLNDKCLHRHCFSCVKQQVKVKLRSGIV-PPCLEDGCK 232

Query: 62  ALLHPDVCHSFVPQNVLDRWGDVLCEAFILGKHRLIYCPFMDCSVALIDDGDEATKEAE- 121
           + L  + C   +   +++ W   + E  I    + IYCP+  CS+ +     E ++EAE 
Sbjct: 233 SELTLESCSMVLTPKLIEMWKRKMEEDLIPDAEK-IYCPYRSCSMLM--SKTELSREAEQ 292

Query: 122 -----CPSCNRMFCAKCNVVWHGG------------------------------------ 158
                C  C+ +FC  C V WH                                      
Sbjct: 293 SNVRACIKCSELFCIDCKVPWHSDLSCADYKRIHSERLVNDMMLKVLANDQMWRQCSECK 352

The following BLAST results are available for this feature:

BLAST of Chy9G157410 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVX0	6.6e-13	28.97	Probable E3 ubiquitin-protein ligase ARI3 OS=Arabidopsis thaliana OX=3702 GN=ARI...	[more]
Q9SKC4	2.6e-09	22.17	Probable E3 ubiquitin-protein ligase ARI10 OS=Arabidopsis thaliana OX=3702 GN=AR...	[more]
A2VEA3	5.8e-09	29.91	E3 ubiquitin-protein ligase ARIH1 OS=Bos taurus OX=9913 GN=ARIH1 PE=2 SV=1	[more]
Q9Y4X5	5.8e-09	29.91	E3 ubiquitin-protein ligase ARIH1 OS=Homo sapiens OX=9606 GN=ARIH1 PE=1 SV=2	[more]
Q9Z1K5	5.8e-09	29.91	E3 ubiquitin-protein ligase ARIH1 OS=Mus musculus OX=10090 GN=Arih1 PE=1 SV=3	[more]

BLAST of Chy9G157410 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KSV5	1.5e-89	72.52	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G650600 PE=3 SV=1	[more]
A0A5A7TCD1	2.4e-82	66.52	Putative E3 ubiquitin-protein ligase RNF217 isoform X2 OS=Cucumis melo var. maku...	[more]
A0A6J1GX07	4.4e-44	43.98	probable E3 ubiquitin-protein ligase ARI10 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1DGR7	9.8e-44	65.41	E3 ubiquitin-protein ligase RNF144A-like OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1IU95	2.0e-41	42.52	probable E3 ubiquitin-protein ligase ARI5 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of Chy9G157410 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141646.1	1.43e-114	72.52	probable E3 ubiquitin-protein ligase ARI3 [Cucumis sativus]	[more]
KAE8649011.1	1.03e-109	72.02	hypothetical protein Csa_009107 [Cucumis sativus]	[more]
KAA0039247.1	3.86e-105	66.52	putative E3 ubiquitin-protein ligase RNF217 isoform X2 [Cucumis melo var. makuwa...	[more]
XP_022956145.1	3.42e-54	44.19	probable E3 ubiquitin-protein ligase ARI10 [Cucurbita moschata]	[more]
XP_022152679.1	4.74e-54	66.15	E3 ubiquitin-protein ligase RNF144A-like [Momordica charantia]	[more]

BLAST of Chy9G157410 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53690.1	2.8e-35	42.75	RING/U-box superfamily protein	[more]
AT3G14250.1	4.2e-31	30.58	RING/U-box superfamily protein	[more]
AT5G60250.1	2.7e-14	30.14	zinc finger (C3HC4-type RING finger) family protein	[more]
AT3G27710.1	4.7e-14	28.97	RING/U-box superfamily protein	[more]
AT5G37560.1	4.0e-13	24.06	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002867	IBR domain	SMART	SM00647	ibrneu5	coord: 79..141 e-value: 6.1E-11 score: 52.4
IPR002867	IBR domain	PFAM	PF01485	IBR	coord: 84..139 e-value: 1.8E-9 score: 37.6
None	No IPR available	GENE3D	2.20.25.20		coord: 97..145 e-value: 5.3E-6 score: 28.4
None	No IPR available	PANTHER	PTHR11685:SF309	RBR-TYPE E3 UBIQUITIN TRANSFERASE	coord: 8..141
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 5..88
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 81..138
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 5..90 e-value: 2.6E-21 score: 77.3
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 11..56 e-value: 1.3E-5 score: 24.9
IPR031127	E3 ubiquitin ligase RBR family	PANTHER	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING	coord: 8..141
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 29..38
IPR044066	TRIAD supradomain	PROSITE	PS51873	TRIAD	coord: 7..170 score: 16.293961
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 11..56 score: 9.789479

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy9G157410.1	Chy9G157410.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity