Chy8G156470 (gene) Cucumber (hystrix) v1

Overview


Name	Chy8G156470
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	pectinesterase inhibitor-like
Location	chrH08: 17531991 .. 17532557 (+)
RNA-Seq Expression	Chy8G156470
Synteny	Chy8G156470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGTCTACTGCCATTGCCGTAGCCTTTTTAGTCATTTCACTTTGCCAGTTCCAAATACTTGCTCAGACTCCACAAACGCCAACCACCTCTGGAGGCAATGATCTTATCTCCAAAACCTGCAGTAGCACACCTTACAGTGAAATGTGTAAAACAATCTTACAATCATCTCCCAACAGCAAAGGAGCAGATTTATATGGCCTAGCACAAATTGTAATGAACATTGCAGCAGATAATGTTAGCAACATATATGAAAACATAAACCTATTACAAAATGGCACATCAGTAGACAGTTTCTTGGATTCGTGTTTGACAGATTGTTTGGAGAGCTTTCAGGACGCTATTGATCAAATTGAAGATTCAGTAACAGCATTGGAATTCAAAGCCTATAATGATGTTAAGACATGGATTTCAGCTGCCATGAGTGATGCAGCTACATGTGATTTGGGGTTCAAAGAAAAACAAGGCTATCAATCTCCAATTGCACAAATGACCTCAGTTTTTGACCAGATTTGCAGCATTATCCTGGCCATTAATCAACTCTTAAGCCAAGGGAATACTAACTAA

mRNA sequence

ATGAAGTCTACTGCCATTGCCGTAGCCTTTTTAGTCATTTCACTTTGCCAGTTCCAAATACTTGCTCAGACTCCACAAACGCCAACCACCTCTGGAGGCAATGATCTTATCTCCAAAACCTGCAGTAGCACACCTTACAGTGAAATGTGTAAAACAATCTTACAATCATCTCCCAACAGCAAAGGAGCAGATTTATATGGCCTAGCACAAATTGTAATGAACATTGCAGCAGATAATGTTAGCAACATATATGAAAACATAAACCTATTACAAAATGGCACATCAGTAGACAGTTTCTTGGATTCGTGTTTGACAGATTGTTTGGAGAGCTTTCAGGACGCTATTGATCAAATTGAAGATTCAGTAACAGCATTGGAATTCAAAGCCTATAATGATGTTAAGACATGGATTTCAGCTGCCATGAGTGATGCAGCTACATGTGATTTGGGGTTCAAAGAAAAACAAGGCTATCAATCTCCAATTGCACAAATGACCTCAGTTTTTGACCAGATTTGCAGCATTATCCTGGCCATTAATCAACTCTTAAGCCAAGGGAATACTAACTAA

Coding sequence (CDS)

ATGAAGTCTACTGCCATTGCCGTAGCCTTTTTAGTCATTTCACTTTGCCAGTTCCAAATACTTGCTCAGACTCCACAAACGCCAACCACCTCTGGAGGCAATGATCTTATCTCCAAAACCTGCAGTAGCACACCTTACAGTGAAATGTGTAAAACAATCTTACAATCATCTCCCAACAGCAAAGGAGCAGATTTATATGGCCTAGCACAAATTGTAATGAACATTGCAGCAGATAATGTTAGCAACATATATGAAAACATAAACCTATTACAAAATGGCACATCAGTAGACAGTTTCTTGGATTCGTGTTTGACAGATTGTTTGGAGAGCTTTCAGGACGCTATTGATCAAATTGAAGATTCAGTAACAGCATTGGAATTCAAAGCCTATAATGATGTTAAGACATGGATTTCAGCTGCCATGAGTGATGCAGCTACATGTGATTTGGGGTTCAAAGAAAAACAAGGCTATCAATCTCCAATTGCACAAATGACCTCAGTTTTTGACCAGATTTGCAGCATTATCCTGGCCATTAATCAACTCTTAAGCCAAGGGAATACTAACTAA

Protein sequence

MKSTAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLISKTCSSTPYSEMCKTILQSSPNSKGADLYGLAQIVMNIAADNVSNIYENINLLQNGTSVDSFLDSCLTDCLESFQDAIDQIEDSVTALEFKAYNDVKTWISAAMSDAATCDLGFKEKQGYQSPIAQMTSVFDQICSIILAINQLLSQGNTN*

Homology

[Close]

BLAST of Chy8G156470 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.3e-13
Identity = 55/178 (30.90%), Postives = 84/178 (47.19%), Query Frame = 0


Query: 11  LVISLCQFQILAQTPQTPTTSGGNDLISKTC------SSTPYSEMCKTILQSSPNSKGAD 70
           L  SLC F +++            D++  TC      S     + C   L + P S  AD
Sbjct: 3   LSFSLCIFFLISA-----------DIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSAD 62

Query: 71  LYGLAQIVMNIAADNVSNIYENINLLQNGTSVDSFLDSCLTDCLESFQDAIDQIEDSVTA 130
           L GL  I  N+A    S I   I  +   + VD  L   L DC+  + DA   +++++  
Sbjct: 63  LQGLGVISANLAIQQGSKIQTFIGRILK-SKVDPALKKYLNDCVGLYADAKSSVQEAIAD 122

Query: 131 LEFKAYNDVKTWISAAMSDAATCDLGFKEKQGYQSPIAQMTSVFDQICSIILAINQLL 183
            + K Y      +SAA+ D+ TC+ GFKEK+G  SP+ +    + Q+ +I LAI +LL
Sbjct: 123 FKSKDYASANVKMSAALDDSVTCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKLL 168

[Close]

BLAST of Chy8G156470 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.3e-13
Identity = 50/154 (32.47%), Postives = 77/154 (50.00%), Query Frame = 0


Query: 35  DLISKTC------SSTPYSEMCKTILQSSPNSKGADLYGLAQIVMNIAADNVSNIYENIN 94
           D++  TC      S     + C   L + P S  ADL GL  I  N+A  + S I   I 
Sbjct: 25  DIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGVISANLAIQHGSKIQTFIG 84

Query: 95  LLQNGTSVDSFLDSCLTDCLESFQDAIDQIEDSVTALEFKAYNDVKTWISAAMSDAATCD 154
            +   + VD  L   L DC+  + DA   +++++   + K Y      +SAA+ D+ TC+
Sbjct: 85  RILK-SKVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCE 144

Query: 155 LGFKEKQGYQSPIAQMTSVFDQICSIILAINQLL 183
            GFKEK+G  SP+ +    + Q+ +I LAI +LL
Sbjct: 145 DGFKEKKGIVSPVTKENKDYVQLTAISLAITKLL 177

[Close]

BLAST of Chy8G156470 vs. ExPASy Swiss-Prot
Match: Q9SB38 (Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 8.3e-09
Identity = 45/161 (27.95%), Postives = 77/161 (47.83%), Query Frame = 0


Query: 10  FLVISLCQFQIL--AQTPQTPTTSGG-----NDLISKTCSSTPYSEMCKTILQSSPNSKG 69
           F+V+SL     +  +  P+T  T  G        +   C+ST Y  MC   L S  ++  
Sbjct: 4   FVVLSLTLMVFINSSNFPKTAATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSSTIK 63

Query: 70  ADLYGLAQIVMNIAADNVSNIYENI-NLLQNGTSVDSFLDSCLTDCLESFQDAIDQIEDS 129
           +D   L    +N+   +  N    + NLLQ   +  S   S L DC++  +D ID+++ +
Sbjct: 64  SDPIKLCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDELKQA 123

Query: 130 VTALEF---------KAYNDVKTWISAAMSDAATCDLGFKE 154
           V  +++         +   +VKTW+S+A++D  TC  GF+E
Sbjct: 124 VAEMKYVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEE 164

[Close]

BLAST of Chy8G156470 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.0e-06
Identity = 56/193 (29.02%), Postives = 85/193 (44.04%), Query Frame = 0


Query: 2   KSTAIAVAFLVISLCQFQILA-------QTPQTPTTSGGNDLISKTCSSTPYSEMCKTIL 61
           K     +A LV+ +  F I A          +   T      IS+TCS + Y  +C   L
Sbjct: 33  KLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTL 92

Query: 62  QSSPNSKGADLYGLAQIVMNIAADNVSN-IY--ENINLLQNGTSVDSFLDSCLTDCLESF 121
              P S  AD   L  I  N      S  +Y    I   Q    V S  DSCL + L+  
Sbjct: 93  LDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQMPPRVRSAYDSCL-ELLDDS 152

Query: 122 QDAIDQIEDSVTALE-FKAYNDVKTWISAAMSDAATCDLGFKEKQGYQSPIAQMTSVFDQ 181
            DA+ +   SV  +   ++++DV TW+S+AM++  TC  GF E +G      Q   V DQ
Sbjct: 153 VDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDGFDEIEG------QGGEVKDQ 212

Query: 182 ICSIILAINQLLS 184
           +   +  +++++S
Sbjct: 213 VIGAVKDLSEMVS 218

[Close]

BLAST of Chy8G156470 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.7e-06
Identity = 36/146 (24.66%), Postives = 66/146 (45.21%), Query Frame = 0


Query: 37  ISKTCSSTPYSEMCKTILQSSPNSKGADLYGLAQIVMNIAADNVSNIYENINLLQNGTSV 96
           I   C        C + ++S+P + GADL  LA I    A  + S  +  I  L   T+ 
Sbjct: 30  IKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVK-TAT 89

Query: 97  DSFLDSCLTDCLESFQDAIDQIEDSVTALEFKAYNDVKTWISAAMSDAATCDLGFKEKQG 156
           +  +    T C++ ++ AI  + D+  +L       +   +SAAM   +TC+    + + 
Sbjct: 90  NPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQDMADFKV 149

Query: 157 YQSPIAQMTSVFDQICSIILAINQLL 183
             S + + +  F  IC I+L I+ ++
Sbjct: 150 DPSAV-KNSGDFQNICGIVLVISNMM 173

[Close]

BLAST of Chy8G156470 vs. ExPASy TrEMBL
Match: A0A0A0M1H8 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569235 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 3.3e-93
Identity = 179/188 (95.21%), Postives = 183/188 (97.34%), Query Frame = 0


Query: 1   MKSTAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLISKTCSSTPYSEMCKTILQSSPNS 60
           MKS AIAVAFLV+SLCQFQILAQTPQTPTTSGGNDLISKTCSST YSEMCKTILQSSPNS
Sbjct: 1   MKSIAIAVAFLVVSLCQFQILAQTPQTPTTSGGNDLISKTCSSTSYSEMCKTILQSSPNS 60

Query: 61  KGADLYGLAQIVMNIAADNVSNIYENINLLQNGTSVDSFLDSCLTDCLESFQDAIDQIED 120
           KGADLYGLAQIVMN+AADNVS+IYENIN LQNGTSVDSFLDSCLTDCLESFQDAIDQIED
Sbjct: 61  KGADLYGLAQIVMNVAADNVSSIYENINQLQNGTSVDSFLDSCLTDCLESFQDAIDQIED 120

Query: 121 SVTALEFKAYNDVKTWISAAMSDAATCDLGFKEKQGYQSPIAQMTSVFDQICSIILAINQ 180
           SVTALEFKAYNDVKTWISAAMSD ATCD GFKEKQGYQSPIAQMTSVFDQICSIIL+INQ
Sbjct: 121 SVTALEFKAYNDVKTWISAAMSDVATCDSGFKEKQGYQSPIAQMTSVFDQICSIILSINQ 180

Query: 181 LLSQGNTN 189
           LLSQGNTN
Sbjct: 181 LLSQGNTN 188

[Close]

BLAST of Chy8G156470 vs. ExPASy TrEMBL
Match: A0A5A7UBK3 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004170 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 3.4e-90
Identity = 172/188 (91.49%), Postives = 182/188 (96.81%), Query Frame = 0


Query: 1   MKSTAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLISKTCSSTPYSEMCKTILQSSPNS 60
           MKS AIAVAFLVISLCQFQILAQTPQTPTTSGGNDLI+KTCSSTP+ EMCKTILQSSPNS
Sbjct: 1   MKSIAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLITKTCSSTPHVEMCKTILQSSPNS 60

Query: 61  KGADLYGLAQIVMNIAADNVSNIYENINLLQNGTSVDSFLDSCLTDCLESFQDAIDQIED 120
           KGADLYGLAQIVMNIAA NVSNIYE+IN LQNGT+VDSFLDSCLTDCLES+QDAIDQIED
Sbjct: 61  KGADLYGLAQIVMNIAASNVSNIYESINQLQNGTTVDSFLDSCLTDCLESYQDAIDQIED 120

Query: 121 SVTALEFKAYNDVKTWISAAMSDAATCDLGFKEKQGYQSPIAQMTSVFDQICSIILAINQ 180
           SVTALEFKAYNDV TW+SAAMSDAATCD+GFKEKQGYQSPIAQMT+VFDQIC IILAIN+
Sbjct: 121 SVTALEFKAYNDVNTWVSAAMSDAATCDMGFKEKQGYQSPIAQMTTVFDQICKIILAINK 180

Query: 181 LLSQGNTN 189
           LLSQGN+N
Sbjct: 181 LLSQGNSN 188

[Close]

BLAST of Chy8G156470 vs. ExPASy TrEMBL
Match: A0A1S3BQL1 (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103492143 PE=4 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.6e-71
Identity = 141/155 (90.97%), Postives = 149/155 (96.13%), Query Frame = 0


Query: 1   MKSTAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLISKTCSSTPYSEMCKTILQSSPNS 60
           MKS AIAVAFLVISLCQFQILAQTPQTPTTSGGNDLI+KTCSSTP+ EMCKTILQSSPNS
Sbjct: 1   MKSIAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLITKTCSSTPHVEMCKTILQSSPNS 60

Query: 61  KGADLYGLAQIVMNIAADNVSNIYENINLLQNGTSVDSFLDSCLTDCLESFQDAIDQIED 120
           KGADLYGLAQIVMNIAA NVSNIYE+IN LQNGT+VDSFLDSCLTDCLES+QDAIDQIED
Sbjct: 61  KGADLYGLAQIVMNIAASNVSNIYESINQLQNGTTVDSFLDSCLTDCLESYQDAIDQIED 120

Query: 121 SVTALEFKAYNDVKTWISAAMSDAATCDLGFKEKQ 156
           SVTALEFKAYNDV TW+SAAMSDAATCD+GFKE +
Sbjct: 121 SVTALEFKAYNDVNTWVSAAMSDAATCDMGFKENK 155

[Close]

BLAST of Chy8G156470 vs. ExPASy TrEMBL
Match: A0A6J1C1M8 (putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111007563 PE=4 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.9e-65
Identity = 132/187 (70.59%), Postives = 155/187 (82.89%), Query Frame = 0


Query: 1   MKSTAIAVAFLVISLCQFQILAQ--TPQTPTTS--GGNDLISKTCSSTPYSEMCKTILQS 60
           M   +I V  LV+SLCQFQILA     Q P  S  GGN+LI+KTC+STPY + C +ILQS
Sbjct: 1   MNPISILVPILVLSLCQFQILADQLQQQQPAASGGGGNELITKTCTSTPYKDFCISILQS 60

Query: 61  SPNSKGADLYGLAQIVMNIAADNVSNIYENINLLQNGTSVDSFLDSCLTDCLESFQDAID 120
           SP S GADLYGLA+I+M  AA NVS+IYE+IN LQN +S D+F++ CL DCLESFQDAID
Sbjct: 61  SPKSNGADLYGLAEILMETAAQNVSSIYESINHLQNDSSPDAFMEQCLADCLESFQDAID 120

Query: 121 QIEDSVTALEFKAYNDVKTWISAAMSDAATCDLGFKEKQGYQSPIAQMTSVFDQICSIIL 180
           QIEDSVTALEFKAYND+KTW+SAAMSDAATCD GFK++QGYQSP+AQMT+VFDQICSIIL
Sbjct: 121 QIEDSVTALEFKAYNDIKTWVSAAMSDAATCDQGFKDRQGYQSPVAQMTAVFDQICSIIL 180

Query: 181 AINQLLS 184
           A+  LL+
Sbjct: 181 AVTHLLA 187

[Close]

BLAST of Chy8G156470 vs. ExPASy TrEMBL
Match: A0A6J1I2M6 (pectinesterase inhibitor 7-like OS=Cucurbita maxima OX=3661 GN=LOC111469035 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 3.1e-59
Identity = 118/189 (62.43%), Postives = 147/189 (77.78%), Query Frame = 0


Query: 1   MKSTAIAVAFL-VISLCQFQILAQTPQTPTTSGGNDLISKTCSSTPYSEMCKTILQSSPN 60
           MK  ++AVA L V+SLC FQ            G N+LI+K CS T Y+++CK ILQ++PN
Sbjct: 1   MKPISVAVAVLVVVSLCSFQ----------AQGDNELITKACSRTAYNDLCKAILQAAPN 60

Query: 61  SKGADLYGLAQIVMNIAADNVSNIYENINLLQNGTSVDSFLDSCLTDCLESFQDAIDQIE 120
           S  ADLYGL Q  ++IAA NVSNIY+ I+ LQN +SVDSF+D CLTDCLE++QDA DQIE
Sbjct: 61  SNDADLYGLTQFALSIAAQNVSNIYQTIDKLQNDSSVDSFMDECLTDCLENYQDATDQIE 120

Query: 121 DSVTALEFKAYNDVKTWISAAMSDAATCDLGFKEKQGYQSPIAQMTSVFDQICSIILAIN 180
           DSVTALEFKA+NDV+TW++AAMSD ATCDLGFK+K GYQSP+ QM++VFDQICSI+L  N
Sbjct: 121 DSVTALEFKAFNDVRTWVAAAMSDVATCDLGFKDKPGYQSPVHQMSAVFDQICSIVLTFN 179

Query: 181 QLLSQGNTN 189
             L +GN +
Sbjct: 181 HCLIEGNVH 179

[Close]

BLAST of Chy8G156470 vs. NCBI nr
Match: XP_011659828.1 (putative invertase inhibitor [Cucumis sativus] >KGN66046.1 hypothetical protein Csa_007292 [Cucumis sativus])

HSP 1 Score: 349 bits (895), Expect = 1.03e-120
Identity = 179/188 (95.21%), Postives = 183/188 (97.34%), Query Frame = 0


Query: 1   MKSTAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLISKTCSSTPYSEMCKTILQSSPNS 60
           MKS AIAVAFLV+SLCQFQILAQTPQTPTTSGGNDLISKTCSST YSEMCKTILQSSPNS
Sbjct: 1   MKSIAIAVAFLVVSLCQFQILAQTPQTPTTSGGNDLISKTCSSTSYSEMCKTILQSSPNS 60

Query: 61  KGADLYGLAQIVMNIAADNVSNIYENINLLQNGTSVDSFLDSCLTDCLESFQDAIDQIED 120
           KGADLYGLAQIVMN+AADNVS+IYENIN LQNGTSVDSFLDSCLTDCLESFQDAIDQIED
Sbjct: 61  KGADLYGLAQIVMNVAADNVSSIYENINQLQNGTSVDSFLDSCLTDCLESFQDAIDQIED 120

Query: 121 SVTALEFKAYNDVKTWISAAMSDAATCDLGFKEKQGYQSPIAQMTSVFDQICSIILAINQ 180
           SVTALEFKAYNDVKTWISAAMSD ATCD GFKEKQGYQSPIAQMTSVFDQICSIIL+INQ
Sbjct: 121 SVTALEFKAYNDVKTWISAAMSDVATCDSGFKEKQGYQSPIAQMTSVFDQICSIILSINQ 180

Query: 181 LLSQGNTN 188
           LLSQGNTN
Sbjct: 181 LLSQGNTN 188

[Close]

BLAST of Chy8G156470 vs. NCBI nr
Match: KAA0050919.1 (putative invertase inhibitor [Cucumis melo var. makuwa] >TYK10267.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 339 bits (869), Expect = 9.43e-117
Identity = 172/188 (91.49%), Postives = 182/188 (96.81%), Query Frame = 0


Query: 1   MKSTAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLISKTCSSTPYSEMCKTILQSSPNS 60
           MKS AIAVAFLVISLCQFQILAQTPQTPTTSGGNDLI+KTCSSTP+ EMCKTILQSSPNS
Sbjct: 1   MKSIAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLITKTCSSTPHVEMCKTILQSSPNS 60

Query: 61  KGADLYGLAQIVMNIAADNVSNIYENINLLQNGTSVDSFLDSCLTDCLESFQDAIDQIED 120
           KGADLYGLAQIVMNIAA NVSNIYE+IN LQNGT+VDSFLDSCLTDCLES+QDAIDQIED
Sbjct: 61  KGADLYGLAQIVMNIAASNVSNIYESINQLQNGTTVDSFLDSCLTDCLESYQDAIDQIED 120

Query: 121 SVTALEFKAYNDVKTWISAAMSDAATCDLGFKEKQGYQSPIAQMTSVFDQICSIILAINQ 180
           SVTALEFKAYNDV TW+SAAMSDAATCD+GFKEKQGYQSPIAQMT+VFDQIC IILAIN+
Sbjct: 121 SVTALEFKAYNDVNTWVSAAMSDAATCDMGFKEKQGYQSPIAQMTTVFDQICKIILAINK 180

Query: 181 LLSQGNTN 188
           LLSQGN+N
Sbjct: 181 LLSQGNSN 188

[Close]

BLAST of Chy8G156470 vs. NCBI nr
Match: XP_038880337.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 307 bits (787), Expect = 2.96e-104
Identity = 153/188 (81.38%), Postives = 173/188 (92.02%), Query Frame = 0


Query: 1   MKSTAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLISKTCSSTPYSEMCKTILQSSPNS 60
           MK  +I +  LV+SLCQFQIL+QTPQTP+TSGGNDLI+KTCSSTPY++MCK+IL SSPNS
Sbjct: 1   MKPISIVMVVLVLSLCQFQILSQTPQTPSTSGGNDLITKTCSSTPYNDMCKSILLSSPNS 60

Query: 61  KGADLYGLAQIVMNIAADNVSNIYENINLLQNGTSVDSFLDSCLTDCLESFQDAIDQIED 120
            GADLYGLAQIV+ IAA+NVSNIYE+IN LQNGTSVDSFLD CLTDCLES+QDAIDQIED
Sbjct: 61  NGADLYGLAQIVIQIAAENVSNIYESINQLQNGTSVDSFLDDCLTDCLESYQDAIDQIED 120

Query: 121 SVTALEFKAYNDVKTWISAAMSDAATCDLGFKEKQGYQSPIAQMTSVFDQICSIILAINQ 180
           SVTALEFKAYNDVKTW+SAAMSDAATCD+GFKEK GYQSPIAQMT+VFDQIC+IIL++ Q
Sbjct: 121 SVTALEFKAYNDVKTWVSAAMSDAATCDMGFKEKPGYQSPIAQMTAVFDQICNIILSMTQ 180

Query: 181 LLSQGNTN 188
           LLS+   N
Sbjct: 181 LLSEEKIN 188

[Close]

BLAST of Chy8G156470 vs. NCBI nr
Match: XP_008450606.1 (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 277 bits (709), Expect = 9.58e-93
Identity = 141/155 (90.97%), Postives = 149/155 (96.13%), Query Frame = 0


Query: 1   MKSTAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLISKTCSSTPYSEMCKTILQSSPNS 60
           MKS AIAVAFLVISLCQFQILAQTPQTPTTSGGNDLI+KTCSSTP+ EMCKTILQSSPNS
Sbjct: 1   MKSIAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLITKTCSSTPHVEMCKTILQSSPNS 60

Query: 61  KGADLYGLAQIVMNIAADNVSNIYENINLLQNGTSVDSFLDSCLTDCLESFQDAIDQIED 120
           KGADLYGLAQIVMNIAA NVSNIYE+IN LQNGT+VDSFLDSCLTDCLES+QDAIDQIED
Sbjct: 61  KGADLYGLAQIVMNIAASNVSNIYESINQLQNGTTVDSFLDSCLTDCLESYQDAIDQIED 120

Query: 121 SVTALEFKAYNDVKTWISAAMSDAATCDLGFKEKQ 155
           SVTALEFKAYNDV TW+SAAMSDAATCD+GFKE +
Sbjct: 121 SVTALEFKAYNDVNTWVSAAMSDAATCDMGFKENK 155

[Close]

BLAST of Chy8G156470 vs. NCBI nr
Match: XP_022135660.1 (putative invertase inhibitor [Momordica charantia])

HSP 1 Score: 256 bits (655), Expect = 3.88e-84
Identity = 132/187 (70.59%), Postives = 155/187 (82.89%), Query Frame = 0


Query: 1   MKSTAIAVAFLVISLCQFQILAQT--PQTPTTSGG--NDLISKTCSSTPYSEMCKTILQS 60
           M   +I V  LV+SLCQFQILA     Q P  SGG  N+LI+KTC+STPY + C +ILQS
Sbjct: 1   MNPISILVPILVLSLCQFQILADQLQQQQPAASGGGGNELITKTCTSTPYKDFCISILQS 60

Query: 61  SPNSKGADLYGLAQIVMNIAADNVSNIYENINLLQNGTSVDSFLDSCLTDCLESFQDAID 120
           SP S GADLYGLA+I+M  AA NVS+IYE+IN LQN +S D+F++ CL DCLESFQDAID
Sbjct: 61  SPKSNGADLYGLAEILMETAAQNVSSIYESINHLQNDSSPDAFMEQCLADCLESFQDAID 120

Query: 121 QIEDSVTALEFKAYNDVKTWISAAMSDAATCDLGFKEKQGYQSPIAQMTSVFDQICSIIL 180
           QIEDSVTALEFKAYND+KTW+SAAMSDAATCD GFK++QGYQSP+AQMT+VFDQICSIIL
Sbjct: 121 QIEDSVTALEFKAYNDIKTWVSAAMSDAATCDQGFKDRQGYQSPVAQMTAVFDQICSIIL 180

Query: 181 AINQLLS 183
           A+  LL+
Sbjct: 181 AVTHLLA 187

[Close]

BLAST of Chy8G156470 vs. TAIR 10
Match: AT1G55770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 5.7e-13
Identity = 51/187 (27.27%), Postives = 91/187 (48.66%), Query Frame = 0


Query: 1   MKSTAIAVAFLVISLCQFQILAQTPQTPTTSGGNDLISKTCSSTPYSEMCKTILQSSPNS 60
           MK +A  V FL I L  F          T +  + +IS  C  +    +C + + S P S
Sbjct: 1   MKQSA-GVLFLCIVLISF---------VTGNANSGMISDLCKHSDDPNLCLSSITSRPES 60

Query: 61  KGADLYGLAQ----IVMNIAADNVSNIYENINLLQNGTSVDSFLDSCLTDCLESFQDAID 120
              +  G +     I ++ A+ N S     I    +   ++  ++  L DC + +QDA++
Sbjct: 61  --GEFAGTSNQIEIIAISAASANASATSSYIKQKLSNEDLEPAIEDTLEDCQKDYQDAVE 120

Query: 121 QIEDSVTALEFKAYNDVKTWISAAMSDAATCDLGFKEKQGYQSPIAQMTSVFDQICSIIL 180
           Q++DS++A+   A+ DV  W+SAA+S   +C      + G  + ++Q   VF ++C   L
Sbjct: 121 QLDDSISAMLADAHTDVDVWLSAAISAIESCGSALGSRAGNDAELSQRNEVFLKLCKNAL 175

Query: 181 AINQLLS 184
            IN++L+
Sbjct: 181 MINKMLT 175

[Close]

BLAST of Chy8G156470 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.4e-11
Identity = 45/159 (28.30%), Postives = 77/159 (48.43%), Query Frame = 0


Query: 31  SGGNDLISKTCSSTPYS------EMCKTILQSSPNSKGA-DLYGLAQIVMNIAADNVSNI 90
           + G  LI  +C     +       +C T L+++P +K A DL GL       A    + +
Sbjct: 18  ANGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAGLVMASTKNAVTKATTL 77

Query: 91  YENINLLQNGTSVDSFLDSCLTDCLESFQDAIDQIEDSVTALEFKAYNDVKTWISAAMSD 150
              ++ +  G  V+      L DCL+ + DAI  + +++  ++ + Y  VKT +SAAM  
Sbjct: 78  KGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKSRNYPTVKTVLSAAMDT 137

Query: 151 AATCDLGFKEKQGYQSPIAQMTSVFDQICSIILAINQLL 183
            +TC+ GFKE++   SP+ +      Q+  I LA   +L
Sbjct: 138 PSTCETGFKERKA-PSPVTKENDNLYQMILIPLAFTNML 175

[Close]

BLAST of Chy8G156470 vs. TAIR 10
Match: AT3G47670.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 1.8e-11
Identity = 61/203 (30.05%), Postives = 93/203 (45.81%), Query Frame = 0


Query: 2   KSTAIAVAFLVISLCQFQILAQT--------PQTPTTSGGNDLISKTCSSTPYSEMCKTI 61
           + T IA   +V  L    ++A T        P  P  S  +  +   CS T Y E C   
Sbjct: 72  RKTLIATGSVVSLLLILSVVALTAGAFTHSPPHHPPISSAS--LKSLCSVTRYPETCFNS 131

Query: 62  LQSSPNSKGADL--YGLAQIVMNIAADNVSNI---YENINLLQNGTSVDSFLDSCLTDCL 121
           L SS N   + L    + ++ + +AA  +SN+   + +IN        D   D+ + DC+
Sbjct: 132 LSSSLNESDSKLNPESILELSLRVAAKEISNLSISFRSIN--------DMPEDAAVGDCV 191

Query: 122 ESFQDAIDQIEDSVTALEFK------------AYNDVKTWISAAMSDAATCDLGFKEKQG 179
           + + DA+ Q+ DS+T +E K               DVKTWISAAM+D  TC  G +E   
Sbjct: 192 KLYTDALSQLNDSITEIERKKKKGGNNWLTEEVVGDVKTWISAAMTDGETCSDGIEEMGT 251

[Close]

BLAST of Chy8G156470 vs. TAIR 10
Match: AT4G02250.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 1.8e-11
Identity = 37/134 (27.61%), Postives = 69/134 (51.49%), Query Frame = 0


Query: 50  CKTILQSSPNSKGADLYGLAQIVMNIAADNVSNIYENINLLQNGTSVDSFLDSCLTDCLE 109
           C   L S P +  A    L  I ++IA+ N S+    I       +++  L+  L DC +
Sbjct: 26  CVASLTSRPEAATATAPKLGVIALSIASSNASDTSFYIKAKLKQKNLEPALEDTLDDCSK 85

Query: 110 SFQDAIDQIEDSVTALEFKAYNDVKTWISAAMSDAATCDLGFKEKQGYQSPIAQMTSVFD 169
           ++ DA+ Q++DS+ AL   ++ DV  W++ A+SD   C+    ++ G  + +A+  +   
Sbjct: 86  NYLDAVAQLDDSLAALMQNSFIDVDIWLNTAISDGEACENALNDRAGNDAELARRNTNLL 145

Query: 170 QICSIILAINQLLS 184
           ++C   L IN +L+
Sbjct: 146 KLCKDALLINTILT 159

[Close]

BLAST of Chy8G156470 vs. TAIR 10
Match: AT1G70720.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 4.1e-11
Identity = 53/178 (29.78%), Postives = 86/178 (48.31%), Query Frame = 0


Query: 19  QILAQTPQTPTTSGGNDLISKTCSSTPYSEMCKTILQSSPNSKGADLYGLAQIVMNIAAD 78
           QIL   P     S G+D I   C++T Y ++C + L S  NS   D   LA++ +++   
Sbjct: 20  QILTVDPPL-LPSNGSDFIRLACNTTLYPDLCFSTLSSFANSIQNDSNRLARVAISLTLH 79

Query: 79  NVSNIYENINLLQNGTSVDSFLDSCLTDCLESFQDAID-------------QIEDSVTAL 138
           N  ++   ++ LQN  + D      L DC E+ +DA+D                 S+ + 
Sbjct: 80  NTLHL---LSYLQNAYNRD-HPTPVLRDCFENLKDAVDGMRGSMKQMKELVSASGSIESF 139

Query: 139 EFKAYNDVKTWISAAMSDAATCDLGFKEKQGYQSPIAQMTSVFDQICSIILAINQLLS 184
            F+  N VKTW+SAA++D  TC  GFK+       + +  S+ D +CS +  + +L S
Sbjct: 140 RFQMSN-VKTWLSAALTDEYTCTDGFKD-------VHEDDSIKDDVCSRVDDVKKLTS 184

The following BLAST results are available for this feature:

BLAST of Chy8G156470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A9YUH4	3.3e-13	30.90	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q8GT41	4.3e-13	32.47	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
Q9SB38	8.3e-09	27.95	Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1	[more]
Q9FK05	1.0e-06	29.02	Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3...	[more]
Q9LUV1	1.7e-06	24.66	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]

BLAST of Chy8G156470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M1H8	3.3e-93	95.21	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569235 PE=4 S...	[more]
A0A5A7UBK3	3.4e-90	91.49	Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3BQL1	1.6e-71	90.97	putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103492143 PE=4 SV=1	[more]
A0A6J1C1M8	2.9e-65	70.59	putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111007563 PE=4...	[more]
A0A6J1I2M6	3.1e-59	62.43	pectinesterase inhibitor 7-like OS=Cucurbita maxima OX=3661 GN=LOC111469035 PE=4...	[more]

BLAST of Chy8G156470 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011659828.1	1.03e-120	95.21	putative invertase inhibitor [Cucumis sativus] >KGN66046.1 hypothetical protein ...	[more]
KAA0050919.1	9.43e-117	91.49	putative invertase inhibitor [Cucumis melo var. makuwa] >TYK10267.1 putative inv...	[more]
XP_038880337.1	2.96e-104	81.38	putative invertase inhibitor [Benincasa hispida]	[more]
XP_008450606.1	9.58e-93	90.97	PREDICTED: putative invertase inhibitor [Cucumis melo]	[more]
XP_022135660.1	3.88e-84	70.59	putative invertase inhibitor [Momordica charantia]	[more]

BLAST of Chy8G156470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55770.1	5.7e-13	27.27	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46940.1	1.4e-11	28.30	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47670.1	1.8e-11	30.05	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G02250.1	1.8e-11	27.61	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G70720.1	4.1e-11	29.78	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 32..178 e-value: 3.7E-30 score: 116.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 35..178 e-value: 2.0E-33 score: 115.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 10..182 e-value: 3.8E-40 score: 135.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 33..182 e-value: 5.0E-42 score: 145.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 34..183
None	No IPR available	PANTHER	PTHR31080:SF219	CELL WALL / VACUOLAR INHIBITOR OF FRUCTOSIDASE 2-LIKE	coord: 15..181
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 15..181
None	No IPR available	CDD	cd15801	PMEI-like_1	coord: 36..182 e-value: 9.239E-51 score: 158.649

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy8G156470.1	Chy8G156470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity