Chy8G156220 (gene) Cucumber (hystrix) v1

Overview
NameChy8G156220
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionS-adenosyl-L-methionine-dependent methyltransferases superfamily protein
LocationchrH08: 17374391 .. 17375812 (-)
RNA-Seq ExpressionChy8G156220
SyntenyChy8G156220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAAGCTGTTCCATACCAAAGGCAGACTAGGAAGAACAATGGGTTGTCTTCAAATTCTATTAATAGGACTTGTTATCATCATGACCACATTGAGTCTCTTCAAGTTCTACTCAGCTGGATTTATCTTTCACAAGGATGATGTTTGCCGATACTTCTACACCACAACGAGGCACGTCTACGAAGAAACGTTCGATGCAAATGCGTTATCCAACCGAGTCGAAGAAGTTCTAGATCAGCTAGAAAGTTTACAAGAGAAACTCGAATTCGCAGTCGAAGAAATGGGAAAGAACAAGCAAGTGTTGAACAATGGGAAGATCACAATGATTGAGCATAAAAAGTACTTAGAAGATGAAGTGATTAAGCCTCTTTATAATGCTCATATTGCTTTAAGGCAAATAAGATTACCAAAAGTTAAAAAAAATGGTCAGAATTCTTCTGCTATGAAAGAAGAGCCTTTGATCAACAATTTTGTGGTTGAGGAAATAAGAAAATATATTTCTCCAAAGAAAAGTAGAATTGGGAAACTCAATATGTATATGACTGAGAATATATACAACACAATTGGACATGCTTGTGTTCTGTATAGGAAAGAATTGGAAGATTATATGGATTATGATATTGGCTCATATTGTAAAGATGATTGGAATTTAGCTCAGAAACTTATGCTCAACGGTTGCGATCCGTTGCCTCGTCGACGGTGCTTGACAAGAGCGTCGAAGGTCTACCAAAAGCCGTATCCATTGAACGAATCGCTATGGAAGATGCCGGACGATCGGAACGTAAGATGGAGCACCTATCAATGTAGGAACTTTTCATGCTTGTCTAGCAACAATCCCAAAAAGGGATACAACAAATGTAGTGGTTGTTTTGAAATGGAGAAAGAGAAGCTCAAATGGGTCATTAGCAATAGCTCTCTCGCAGTCGATTTTGCGATATCGGATGTATTAGCGATCAAGCCGGGGGAGATTCGGATCGGGTTGGATTTTGGGATCAGCACGGGGAGTTTCGCAGCGAGAATGAGAGAAGAAAATGTGACAATTGTGACAACAGCTTTGAACCTTGGAGCTCCTTTCAATGAATTTATTGCTTTAAGAGGTTTGTTTCCACTGTATTTGACATTAAACCAAAGACTTCCTCTGTTTGATAACACAATGGATTTGATTCACACAACTGGTTTCATGGATGGTTGGCTAGATATGTTATTGTTGGATTTCATACTTTTCGATTGGGATCGTGTTCTACGACCGGGAGGATTGTTATGGATTGACAGATTCTTTTGCGATCGAAAGGATCTCGACGATTACATGTACATGTTTCTGCAGTTTAGATACAAGAAACATAAATGGAGTATTGCTCCTAAATCAAGAAATGAAGTCTACCTTTCTGCATTGTTGGAAAAACCTCCAAGGGCAATATGA

mRNA sequence

ATGAAGAAGCTGTTCCATACCAAAGGCAGACTAGGAAGAACAATGGGTTGTCTTCAAATTCTATTAATAGGACTTGTTATCATCATGACCACATTGAGTCTCTTCAAGTTCTACTCAGCTGGATTTATCTTTCACAAGGATGATGTTTGCCGATACTTCTACACCACAACGAGGCACGTCTACGAAGAAACGTTCGATGCAAATGCGTTATCCAACCGAGTCGAAGAAGTTCTAGATCAGCTAGAAAGTTTACAAGAGAAACTCGAATTCGCAGTCGAAGAAATGGGAAAGAACAAGCAAGTGTTGAACAATGGGAAGATCACAATGATTGAGCATAAAAAGTACTTAGAAGATGAAGTGATTAAGCCTCTTTATAATGCTCATATTGCTTTAAGGCAAATAAGATTACCAAAAGTTAAAAAAAATGGTCAGAATTCTTCTGCTATGAAAGAAGAGCCTTTGATCAACAATTTTGTGGTTGAGGAAATAAGAAAATATATTTCTCCAAAGAAAAGTAGAATTGGGAAACTCAATATGTATATGACTGAGAATATATACAACACAATTGGACATGCTTGTGTTCTGTATAGGAAAGAATTGGAAGATTATATGGATTATGATATTGGCTCATATTGTAAAGATGATTGGAATTTAGCTCAGAAACTTATGCTCAACGGTTGCGATCCGTTGCCTCGTCGACGGTGCTTGACAAGAGCGTCGAAGGTCTACCAAAAGCCGTATCCATTGAACGAATCGCTATGGAAGATGCCGGACGATCGGAACGTAAGATGGAGCACCTATCAATGTAGGAACTTTTCATGCTTGTCTAGCAACAATCCCAAAAAGGGATACAACAAATGTAGTGGTTGTTTTGAAATGGAGAAAGAGAAGCTCAAATGGGTCATTAGCAATAGCTCTCTCGCAGTCGATTTTGCGATATCGGATGTATTAGCGATCAAGCCGGGGGAGATTCGGATCGGGTTGGATTTTGGGATCAGCACGGGGAGTTTCGCAGCGAGAATGAGAGAAGAAAATGTGACAATTGTGACAACAGCTTTGAACCTTGGAGCTCCTTTCAATGAATTTATTGCTTTAAGAGGTTTGTTTCCACTGTATTTGACATTAAACCAAAGACTTCCTCTGTTTGATAACACAATGGATTTGATTCACACAACTGGTTTCATGGATGGTTGGCTAGATATGTTATTGTTGGATTTCATACTTTTCGATTGGGATCGTGTTCTACGACCGGGAGGATTGTTATGGATTGACAGATTCTTTTGCGATCGAAAGGATCTCGACGATTACATGTACATGTTTCTGCAGTTTAGATACAAGAAACATAAATGGAGTATTGCTCCTAAATCAAGAAATGAAGTCTACCTTTCTGCATTGTTGGAAAAACCTCCAAGGGCAATATGA

Coding sequence (CDS)

ATGAAGAAGCTGTTCCATACCAAAGGCAGACTAGGAAGAACAATGGGTTGTCTTCAAATTCTATTAATAGGACTTGTTATCATCATGACCACATTGAGTCTCTTCAAGTTCTACTCAGCTGGATTTATCTTTCACAAGGATGATGTTTGCCGATACTTCTACACCACAACGAGGCACGTCTACGAAGAAACGTTCGATGCAAATGCGTTATCCAACCGAGTCGAAGAAGTTCTAGATCAGCTAGAAAGTTTACAAGAGAAACTCGAATTCGCAGTCGAAGAAATGGGAAAGAACAAGCAAGTGTTGAACAATGGGAAGATCACAATGATTGAGCATAAAAAGTACTTAGAAGATGAAGTGATTAAGCCTCTTTATAATGCTCATATTGCTTTAAGGCAAATAAGATTACCAAAAGTTAAAAAAAATGGTCAGAATTCTTCTGCTATGAAAGAAGAGCCTTTGATCAACAATTTTGTGGTTGAGGAAATAAGAAAATATATTTCTCCAAAGAAAAGTAGAATTGGGAAACTCAATATGTATATGACTGAGAATATATACAACACAATTGGACATGCTTGTGTTCTGTATAGGAAAGAATTGGAAGATTATATGGATTATGATATTGGCTCATATTGTAAAGATGATTGGAATTTAGCTCAGAAACTTATGCTCAACGGTTGCGATCCGTTGCCTCGTCGACGGTGCTTGACAAGAGCGTCGAAGGTCTACCAAAAGCCGTATCCATTGAACGAATCGCTATGGAAGATGCCGGACGATCGGAACGTAAGATGGAGCACCTATCAATGTAGGAACTTTTCATGCTTGTCTAGCAACAATCCCAAAAAGGGATACAACAAATGTAGTGGTTGTTTTGAAATGGAGAAAGAGAAGCTCAAATGGGTCATTAGCAATAGCTCTCTCGCAGTCGATTTTGCGATATCGGATGTATTAGCGATCAAGCCGGGGGAGATTCGGATCGGGTTGGATTTTGGGATCAGCACGGGGAGTTTCGCAGCGAGAATGAGAGAAGAAAATGTGACAATTGTGACAACAGCTTTGAACCTTGGAGCTCCTTTCAATGAATTTATTGCTTTAAGAGGTTTGTTTCCACTGTATTTGACATTAAACCAAAGACTTCCTCTGTTTGATAACACAATGGATTTGATTCACACAACTGGTTTCATGGATGGTTGGCTAGATATGTTATTGTTGGATTTCATACTTTTCGATTGGGATCGTGTTCTACGACCGGGAGGATTGTTATGGATTGACAGATTCTTTTGCGATCGAAAGGATCTCGACGATTACATGTACATGTTTCTGCAGTTTAGATACAAGAAACATAAATGGAGTATTGCTCCTAAATCAAGAAATGAAGTCTACCTTTCTGCATTGTTGGAAAAACCTCCAAGGGCAATATGA

Protein sequence

MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHVYEETFDANALSNRVEEVLDQLESLQEKLEFAVEEMGKNKQVLNNGKITMIEHKKYLEDEVIKPLYNAHIALRQIRLPKVKKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRASKVYQKPYPLNESLWKMPDDRNVRWSTYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKLKWVISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFNEFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSRNEVYLSALLEKPPRAI*
Homology
BLAST of Chy8G156220 vs. ExPASy Swiss-Prot
Match: Q8RWB7 (Probable methyltransferase At1g29790 OS=Arabidopsis thaliana OX=3702 GN=CLSC5 PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.1e-53
Identity = 119/288 (41.32%), Postives = 168/288 (58.33%), Query Frame = 0

Query: 189 IGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRASKVYQKPYP 248
           I  AC  Y   L +YM+Y   S C  D +L +KL+L GC PLPRRRC +R         P
Sbjct: 105 IASACHNYPDLLHEYMNYTPFSLCPSDTDLVEKLILRGCHPLPRRRCFSRT--------P 164

Query: 249 LNESLWKMPDDRNVRWSTYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKLKWVISNSSLA 308
            N S  K   + NV WS Y C++F CL +     G       F++  EK K   S     
Sbjct: 165 RNPSDSK--PESNVLWSYYSCKSFDCLITKFSDLG-------FDLSLEKSKSQFSAYKSE 224

Query: 309 VDFAISDVLAIKPGE---IRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFNEFIAL 368
           +D  IS +L I       +R+G+D G  TGSFAA M+  NVT++TT +N  AP++E +A+
Sbjct: 225 LDLPISQLLQIAKSANSVLRLGIDVGGGTGSFAAAMKARNVTVLTTTMNFNAPYSEAVAM 284

Query: 369 RGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDR 428
           RGL PL++ L QRLP+FD  +DL+     ++ W+ + +++F  FD DR+LR GG LW+DR
Sbjct: 285 RGLVPLHVPLQQRLPVFDGVVDLVRCGRAVNRWIPVTVMEFFFFDLDRILRGGGYLWLDR 344

Query: 429 FFCDRKDLDD-YMYMFLQFRYKKHKWSIAPKS---RNEVYLSALLEKP 470
           FF  + DL++ Y  M  +  YKK KW++A K+     EV+L+ALL+KP
Sbjct: 345 FFSKKVDLENVYAPMIGKLGYKKVKWAVANKADSKHGEVFLTALLQKP 375

BLAST of Chy8G156220 vs. ExPASy TrEMBL
Match: A0A5D3CEF4 (Putative Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003900 PE=3 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 8.7e-268
Identity = 457/473 (96.62%), Postives = 462/473 (97.67%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60
           MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV
Sbjct: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60

Query: 61  YEETFDANALSNRVEEVLDQLESLQEKLEFAVEEMGKNKQVLNNGKITMIEHKKYLEDEV 120
           YEETFDA ALSNRVEEVLDQLESLQEKLE AVEEM KNKQVLNNG I MIEHKKYLEDEV
Sbjct: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120

Query: 121 IKPLYNAHIALRQIRLPKVKKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           IKPLYNAHIALRQIRLPKV KNGQNSS MKEEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240
           MTENIYNTIGHACVLY+KELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS
Sbjct: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240

Query: 241 KVYQKPYPLNESLWKMPDDRNVRWSTYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKLKW 300
           KVYQKPYPLNESLWK PDDRNVRWS YQCRNFSCLSS NPK+GYNKCSGCFE+EKEKLKW
Sbjct: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEIEKEKLKW 300

Query: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360
           VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN
Sbjct: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGLFPLY TLNQRLPLFDNTMDLIHT+GFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSRNEVYLSALLEKPPRAI 474
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKS+NEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 473

BLAST of Chy8G156220 vs. ExPASy TrEMBL
Match: A0A1S3BQC0 (uncharacterized protein LOC103492170 OS=Cucumis melo OX=3656 GN=LOC103492170 PE=3 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 8.7e-268
Identity = 457/473 (96.62%), Postives = 462/473 (97.67%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60
           MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV
Sbjct: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60

Query: 61  YEETFDANALSNRVEEVLDQLESLQEKLEFAVEEMGKNKQVLNNGKITMIEHKKYLEDEV 120
           YEETFDA ALSNRVEEVLDQLESLQEKLE AVEEM KNKQVLNNG I MIEHKKYLEDEV
Sbjct: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120

Query: 121 IKPLYNAHIALRQIRLPKVKKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           IKPLYNAHIALRQIRLPKV KNGQNSS MKEEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240
           MTENIYNTIGHACVLY+KELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS
Sbjct: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240

Query: 241 KVYQKPYPLNESLWKMPDDRNVRWSTYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKLKW 300
           KVYQKPYPLNESLWK PDDRNVRWS YQCRNFSCLSS NPK+GYNKCSGCFE+EKEKLKW
Sbjct: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEIEKEKLKW 300

Query: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360
           VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN
Sbjct: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGLFPLY TLNQRLPLFDNTMDLIHT+GFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSRNEVYLSALLEKPPRAI 474
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKS+NEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 473

BLAST of Chy8G156220 vs. ExPASy TrEMBL
Match: A0A6J1CY71 (uncharacterized protein LOC111015349 OS=Momordica charantia OX=3673 GN=LOC111015349 PE=3 SV=1)

HSP 1 Score: 845.1 bits (2182), Expect = 1.4e-241
Identity = 411/473 (86.89%), Postives = 444/473 (93.87%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60
           M+KL HTK RLGRTMG LQILL GLVI++TTLSLFKFYSAGF FHK+DVCRYFY TT+HV
Sbjct: 1   MRKLIHTKCRLGRTMGWLQILLGGLVILITTLSLFKFYSAGFFFHKEDVCRYFY-TTKHV 60

Query: 61  YEETFDANALSNRVEEVLDQLESLQEKLEFAVEEMGKNKQVLNNGKITMIEHKKYLEDEV 120
           YE +FD  ALS+RVEEVLDQL+ LQ KLE AVEEM KNKQVL+NG IT +EHKKYLE+EV
Sbjct: 61  YEASFDVKALSDRVEEVLDQLDGLQAKLESAVEEMEKNKQVLSNGSITRLEHKKYLEEEV 120

Query: 121 IKPLYNAHIALRQIRLPKVKKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           I+PLYNAHIALRQIRLPKV+K    +S M+EEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IRPLYNAHIALRQIRLPKVEKIDDQNSNMQEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240
           MTE IYNTIGHACVL+RKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCL RAS
Sbjct: 181 MTEKIYNTIGHACVLHRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS 240

Query: 241 KVYQKPYPLNESLWKMPDDRNVRWSTYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKLKW 300
           KVYQ+PYP+NESLWK+PDDRNVRWS YQCRNFSCLSS NP++GYNKCSGCFE+EKEK KW
Sbjct: 241 KVYQRPYPVNESLWKLPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFELEKEKRKW 300

Query: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360
           V +NSSL+VDF +SDVLA+K GEIR+GLDFGIS+GSFAARM+E+NVTIVTTALNLGAPFN
Sbjct: 301 V-TNSSLSVDFVLSDVLALKAGEIRVGLDFGISSGSFAARMKEQNVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGL PLY+TLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSRNEVYLSALLEKPPRAI 474
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKW+IAPKS+NEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 471

BLAST of Chy8G156220 vs. ExPASy TrEMBL
Match: A0A6J1I1D3 (uncharacterized protein LOC111468555 OS=Cucurbita maxima OX=3661 GN=LOC111468555 PE=3 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 2.5e-238
Identity = 408/475 (85.89%), Postives = 440/475 (92.63%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFY--TTTR 60
           M+KL HTK R+GRTMGCLQILL GLVI +TTLSLFKFYSAGFIFHK+DVCRYFY  TTT+
Sbjct: 1   MRKLIHTKWRVGRTMGCLQILLGGLVITITTLSLFKFYSAGFIFHKEDVCRYFYTTTTTK 60

Query: 61  HVYEETFDANALSNRVEEVLDQLESLQEKLEFAVEEMGKNKQVLNNGKITMIEHKKYLED 120
            +YE +FDA ALS+RVEEVLDQLESLQ+KLE  VEEM ++KQ L+NG +  IEHKKYLE 
Sbjct: 61  QIYEASFDAKALSDRVEEVLDQLESLQDKLESVVEEMERDKQALSNGNMRRIEHKKYLEM 120

Query: 121 EVIKPLYNAHIALRQIRLPKVKKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLN 180
           EVIKPLYNAHI LRQIRLPKV+K  +NSS MKEEPLINNFVVEEIRKYI+PKKSR GK+N
Sbjct: 121 EVIKPLYNAHIGLRQIRLPKVEKMNENSS-MKEEPLINNFVVEEIRKYITPKKSRTGKVN 180

Query: 181 MYMTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTR 240
           MYMTE+IYNTIGHACVL RKELE+YM YDIGSYCKDDWN+ QKLMLNGCDPLPRRRCLTR
Sbjct: 181 MYMTESIYNTIGHACVLVRKELEEYMSYDIGSYCKDDWNMGQKLMLNGCDPLPRRRCLTR 240

Query: 241 ASKVYQKPYPLNESLWKMPDDRNVRWSTYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKL 300
           ASKVYQKPYP+NESLWK PDDRNVRWS YQCRNFSCLSS NPK+GYNKCSGCFEMEKEK+
Sbjct: 241 ASKVYQKPYPVNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEMEKEKV 300

Query: 301 KWVISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAP 360
           KWV  NSS+AVDF ISDVL +K GEIR+GLDFGISTGSF ARMREENVTIVTTALNLGAP
Sbjct: 301 KWV-RNSSVAVDFRISDVLGMKAGEIRVGLDFGISTGSFGARMREENVTIVTTALNLGAP 360

Query: 361 FNEFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPG 420
           FNEFIALRGL PLY+TLNQRLPLFDNTMDLIHTTGFMDGW+DMLLLDFILFDWDRVLRPG
Sbjct: 361 FNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWVDMLLLDFILFDWDRVLRPG 420

Query: 421 GLLWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSRNEVYLSALLEKPPRAI 474
           GLLWIDRFFCDRKDLDDY+YMFLQFRYKKHKW+IAPKS+NEVYLSA+LEKPPRAI
Sbjct: 421 GLLWIDRFFCDRKDLDDYIYMFLQFRYKKHKWAIAPKSKNEVYLSAVLEKPPRAI 473

BLAST of Chy8G156220 vs. ExPASy TrEMBL
Match: A0A6J1EZZ0 (uncharacterized protein LOC111440913 OS=Cucurbita moschata OX=3662 GN=LOC111440913 PE=3 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 1.8e-236
Identity = 408/478 (85.36%), Postives = 440/478 (92.05%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFY-----T 60
           M+KL HTK R+GRTMGCLQILL GLVI +TTLSLFKFYSAGFIFHK+DVCRYFY     T
Sbjct: 1   MRKLIHTKWRVGRTMGCLQILLGGLVITITTLSLFKFYSAGFIFHKEDVCRYFYTPPTTT 60

Query: 61  TTRHVYEETFDANALSNRVEEVLDQLESLQEKLEFAVEEMGKNKQVLNNGKITMIEHKKY 120
           TT+ VYE +FDA ALS+RVEEVLDQLESLQ+KLE AVEEM ++KQ L+NG +  IEHKKY
Sbjct: 61  TTKQVYEASFDAKALSDRVEEVLDQLESLQDKLESAVEEMERDKQALSNGNMRRIEHKKY 120

Query: 121 LEDEVIKPLYNAHIALRQIRLPKVKKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIG 180
           LE EVIKPLYNAHIALRQIRLPKV+K  +N S +KEEPLINNFVVEEIRKYI+PKKSR G
Sbjct: 121 LEMEVIKPLYNAHIALRQIRLPKVEKVKENLS-VKEEPLINNFVVEEIRKYITPKKSRTG 180

Query: 181 KLNMYMTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRC 240
           K+NMYMTE+IYNTIGHACVL RKELE+YM YDIGSYCKDDWN+ QKLMLNGCDPLPRRRC
Sbjct: 181 KVNMYMTESIYNTIGHACVLVRKELEEYMSYDIGSYCKDDWNVGQKLMLNGCDPLPRRRC 240

Query: 241 LTRASKVYQKPYPLNESLWKMPDDRNVRWSTYQCRNFSCLSSNNPKKGYNKCSGCFEMEK 300
           LTRASKVYQKPY +NESLWK PDDRNVRWS YQCRNFSCLSS NPK+GYNKCSGCFEMEK
Sbjct: 241 LTRASKVYQKPYAVNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEMEK 300

Query: 301 EKLKWVISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNL 360
           EK+KWV  NSS+AVDF ISDVL +K GEIR+GLDFGISTGSF ARMREENVTIVTTALNL
Sbjct: 301 EKVKWV-RNSSVAVDFRISDVLGMKAGEIRVGLDFGISTGSFGARMREENVTIVTTALNL 360

Query: 361 GAPFNEFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVL 420
           GAPFNEFIALRGL PLY+TLNQRLPLFDNTMDLIHTTGFMDGW+DMLLLDFILFDWDRVL
Sbjct: 361 GAPFNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWVDMLLLDFILFDWDRVL 420

Query: 421 RPGGLLWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSRNEVYLSALLEKPPRAI 474
           RPGGLLWIDRFFCDRKDLDDY+YMFLQFRYKKHKW+IAPKS+NEVYLSA+LEKPPRAI
Sbjct: 421 RPGGLLWIDRFFCDRKDLDDYIYMFLQFRYKKHKWAIAPKSKNEVYLSAVLEKPPRAI 476

BLAST of Chy8G156220 vs. NCBI nr
Match: XP_011659893.1 (uncharacterized protein LOC101207467 [Cucumis sativus] >KAE8653367.1 hypothetical protein Csa_006971 [Cucumis sativus])

HSP 1 Score: 946 bits (2445), Expect = 0.0
Identity = 466/473 (98.52%), Postives = 468/473 (98.94%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60
           MKKLFHTKGRLGRTMG LQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRH 
Sbjct: 1   MKKLFHTKGRLGRTMGWLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHA 60

Query: 61  YEETFDANALSNRVEEVLDQLESLQEKLEFAVEEMGKNKQVLNNGKITMIEHKKYLEDEV 120
           YEETFDA ALSNRVEEVLDQLESLQEKLEF VEEMGKNKQVLNNGKITMIEHKKYLEDEV
Sbjct: 61  YEETFDAKALSNRVEEVLDQLESLQEKLEFTVEEMGKNKQVLNNGKITMIEHKKYLEDEV 120

Query: 121 IKPLYNAHIALRQIRLPKVKKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           IKPLYNAHIALRQIRLPKV+KNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IKPLYNAHIALRQIRLPKVEKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240
           MTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS
Sbjct: 181 MTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240

Query: 241 KVYQKPYPLNESLWKMPDDRNVRWSTYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKLKW 300
           KVYQKPYPLNESLWKMPDDRNVRWS YQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKLKW
Sbjct: 241 KVYQKPYPLNESLWKMPDDRNVRWSNYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKLKW 300

Query: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360
           VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN
Sbjct: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSRNEVYLSALLEKPPRAI 473
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKS+NEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 473

BLAST of Chy8G156220 vs. NCBI nr
Match: XP_008450643.1 (PREDICTED: uncharacterized protein LOC103492170 [Cucumis melo] >KAA0050891.1 putative Methyltransferase [Cucumis melo var. makuwa] >TYK10243.1 putative Methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 928 bits (2399), Expect = 0.0
Identity = 457/473 (96.62%), Postives = 462/473 (97.67%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60
           MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV
Sbjct: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60

Query: 61  YEETFDANALSNRVEEVLDQLESLQEKLEFAVEEMGKNKQVLNNGKITMIEHKKYLEDEV 120
           YEETFDA ALSNRVEEVLDQLESLQEKLE AVEEM KNKQVLNNG I MIEHKKYLEDEV
Sbjct: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120

Query: 121 IKPLYNAHIALRQIRLPKVKKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           IKPLYNAHIALRQIRLPKV KNGQNSS MKEEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240
           MTENIYNTIGHACVLY+KELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS
Sbjct: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240

Query: 241 KVYQKPYPLNESLWKMPDDRNVRWSTYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKLKW 300
           KVYQKPYPLNESLWK PDDRNVRWS YQCRNFSCLSS NPK+GYNKCSGCFE+EKEKLKW
Sbjct: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEIEKEKLKW 300

Query: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360
           VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN
Sbjct: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGLFPLY TLNQRLPLFDNTMDLIHT+GFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSRNEVYLSALLEKPPRAI 473
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKS+NEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 473

BLAST of Chy8G156220 vs. NCBI nr
Match: XP_038880508.1 (uncharacterized protein LOC120072172 [Benincasa hispida])

HSP 1 Score: 887 bits (2293), Expect = 0.0
Identity = 437/468 (93.38%), Postives = 452/468 (96.58%), Query Frame = 0

Query: 6   HTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHVYEETF 65
             KGRLGRTMG LQILL GLVII+TTLSLFKFYSAGFIFHKDDVCRYFYTTTRHVYEE+F
Sbjct: 2   RNKGRLGRTMGWLQILLGGLVIIITTLSLFKFYSAGFIFHKDDVCRYFYTTTRHVYEESF 61

Query: 66  DANALSNRVEEVLDQLESLQEKLEFAVEEMGKNKQVLNNGKITMIEHKKYLEDEVIKPLY 125
           DA ALS+RVEEVLDQLESLQEKLE AVEEMGKNKQVLNNG IT IEHKKYLE+EVIKPLY
Sbjct: 62  DAKALSDRVEEVLDQLESLQEKLESAVEEMGKNKQVLNNGNITRIEHKKYLEEEVIKPLY 121

Query: 126 NAHIALRQIRLPKVKKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTENI 185
           NAHIALRQIRLPKV+KNGQN S +KEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTENI
Sbjct: 122 NAHIALRQIRLPKVEKNGQNYSTVKEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTENI 181

Query: 186 YNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRASKVYQK 245
           YNTIGHACVL+RKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRASKVYQK
Sbjct: 182 YNTIGHACVLHRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRASKVYQK 241

Query: 246 PYPLNESLWKMPDDRNVRWSTYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKLKWVISNS 305
           PYP+NESLWK PDDRNVRWS YQCRNFSCLSS NPK+GYNKCSGCFE++KEKLKWVI NS
Sbjct: 242 PYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEIDKEKLKWVI-NS 301

Query: 306 SLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFNEFIAL 365
           SLAVDF ISDVLAIKPGEIRIGLDFGISTGSFAARMRE+NVTIVTTALNLGAPFNEFIAL
Sbjct: 302 SLAVDFTISDVLAIKPGEIRIGLDFGISTGSFAARMREQNVTIVTTALNLGAPFNEFIAL 361

Query: 366 RGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDR 425
           RGL PLY+TLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDR
Sbjct: 362 RGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDR 421

Query: 426 FFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSRNEVYLSALLEKPPRAI 473
           FFCDRKDLDDYMYMFLQFRYKKHKW+IAPKS+NEVYLSALLEKPPRAI
Sbjct: 422 FFCDRKDLDDYMYMFLQFRYKKHKWTIAPKSKNEVYLSALLEKPPRAI 468

BLAST of Chy8G156220 vs. NCBI nr
Match: XP_022146046.1 (uncharacterized protein LOC111015349 [Momordica charantia])

HSP 1 Score: 841 bits (2173), Expect = 5.76e-306
Identity = 411/473 (86.89%), Postives = 444/473 (93.87%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60
           M+KL HTK RLGRTMG LQILL GLVI++TTLSLFKFYSAGF FHK+DVCRYFYTT +HV
Sbjct: 1   MRKLIHTKCRLGRTMGWLQILLGGLVILITTLSLFKFYSAGFFFHKEDVCRYFYTT-KHV 60

Query: 61  YEETFDANALSNRVEEVLDQLESLQEKLEFAVEEMGKNKQVLNNGKITMIEHKKYLEDEV 120
           YE +FD  ALS+RVEEVLDQL+ LQ KLE AVEEM KNKQVL+NG IT +EHKKYLE+EV
Sbjct: 61  YEASFDVKALSDRVEEVLDQLDGLQAKLESAVEEMEKNKQVLSNGSITRLEHKKYLEEEV 120

Query: 121 IKPLYNAHIALRQIRLPKVKKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           I+PLYNAHIALRQIRLPKV+K    +S M+EEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IRPLYNAHIALRQIRLPKVEKIDDQNSNMQEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240
           MTE IYNTIGHACVL+RKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCL RAS
Sbjct: 181 MTEKIYNTIGHACVLHRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS 240

Query: 241 KVYQKPYPLNESLWKMPDDRNVRWSTYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKLKW 300
           KVYQ+PYP+NESLWK+PDDRNVRWS YQCRNFSCLSS NP++GYNKCSGCFE+EKEK KW
Sbjct: 241 KVYQRPYPVNESLWKLPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFELEKEKRKW 300

Query: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360
           V +NSSL+VDF +SDVLA+K GEIR+GLDFGIS+GSFAARM+E+NVTIVTTALNLGAPFN
Sbjct: 301 V-TNSSLSVDFVLSDVLALKAGEIRVGLDFGISSGSFAARMKEQNVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGL PLY+TLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSRNEVYLSALLEKPPRAI 473
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKW+IAPKS+NEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 471

BLAST of Chy8G156220 vs. NCBI nr
Match: XP_022969573.1 (uncharacterized protein LOC111468555 [Cucurbita maxima])

HSP 1 Score: 830 bits (2145), Expect = 1.14e-301
Identity = 408/475 (85.89%), Postives = 440/475 (92.63%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTT--R 60
           M+KL HTK R+GRTMGCLQILL GLVI +TTLSLFKFYSAGFIFHK+DVCRYFYTTT  +
Sbjct: 1   MRKLIHTKWRVGRTMGCLQILLGGLVITITTLSLFKFYSAGFIFHKEDVCRYFYTTTTTK 60

Query: 61  HVYEETFDANALSNRVEEVLDQLESLQEKLEFAVEEMGKNKQVLNNGKITMIEHKKYLED 120
            +YE +FDA ALS+RVEEVLDQLESLQ+KLE  VEEM ++KQ L+NG +  IEHKKYLE 
Sbjct: 61  QIYEASFDAKALSDRVEEVLDQLESLQDKLESVVEEMERDKQALSNGNMRRIEHKKYLEM 120

Query: 121 EVIKPLYNAHIALRQIRLPKVKKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLN 180
           EVIKPLYNAHI LRQIRLPKV+K  +NSS MKEEPLINNFVVEEIRKYI+PKKSR GK+N
Sbjct: 121 EVIKPLYNAHIGLRQIRLPKVEKMNENSS-MKEEPLINNFVVEEIRKYITPKKSRTGKVN 180

Query: 181 MYMTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTR 240
           MYMTE+IYNTIGHACVL RKELE+YM YDIGSYCKDDWN+ QKLMLNGCDPLPRRRCLTR
Sbjct: 181 MYMTESIYNTIGHACVLVRKELEEYMSYDIGSYCKDDWNMGQKLMLNGCDPLPRRRCLTR 240

Query: 241 ASKVYQKPYPLNESLWKMPDDRNVRWSTYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKL 300
           ASKVYQKPYP+NESLWK PDDRNVRWS YQCRNFSCLSS NPK+GYNKCSGCFEMEKEK+
Sbjct: 241 ASKVYQKPYPVNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEMEKEKV 300

Query: 301 KWVISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAP 360
           KWV  NSS+AVDF ISDVL +K GEIR+GLDFGISTGSF ARMREENVTIVTTALNLGAP
Sbjct: 301 KWV-RNSSVAVDFRISDVLGMKAGEIRVGLDFGISTGSFGARMREENVTIVTTALNLGAP 360

Query: 361 FNEFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPG 420
           FNEFIALRGL PLY+TLNQRLPLFDNTMDLIHTTGFMDGW+DMLLLDFILFDWDRVLRPG
Sbjct: 361 FNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWVDMLLLDFILFDWDRVLRPG 420

Query: 421 GLLWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSRNEVYLSALLEKPPRAI 473
           GLLWIDRFFCDRKDLDDY+YMFLQFRYKKHKW+IAPKS+NEVYLSA+LEKPPRAI
Sbjct: 421 GLLWIDRFFCDRKDLDDYIYMFLQFRYKKHKWAIAPKSKNEVYLSAVLEKPPRAI 473

BLAST of Chy8G156220 vs. TAIR 10
Match: AT3G05390.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion; EXPRESSED IN: 15 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF248, methyltransferase putative (InterPro:IPR004159); BEST Arabidopsis thaliana protein match is: S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (TAIR:AT4G01240.1); Has 507 Blast hits to 498 proteins in 33 species: Archae - 4; Bacteria - 8; Metazoa - 0; Fungi - 0; Plants - 493; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 672.2 bits (1733), Expect = 3.1e-193
Identity = 326/465 (70.11%), Postives = 385/465 (82.80%), Query Frame = 0

Query: 10  RLGRTMGCLQILLIGLVIIMTTLS-LFKFYSAGFIFHKDDVCRYFYTTTRHVYEETFDAN 69
           RLGR M  LQ++L GL++I  +LS L++F+S G+    +D C   Y T + V  E FD  
Sbjct: 10  RLGRLMAGLQVIL-GLLVITVSLSCLYRFHSVGYFLQNEDTCNNIY-TIKEVSSEGFDLK 69

Query: 70  ALSNRVEEVLDQLESLQEKLEFAVEEMGKNKQVLNNGKITMIEHKKYLEDEVIKPLYNAH 129
           AL +RV+EVL+++++L EKLE  V++M K+K           E KK+LEDEV+KP Y AH
Sbjct: 70  ALHDRVDEVLEKMDNLYEKLEKTVKDMEKSKDGSKK------EMKKFLEDEVMKPFYYAH 129

Query: 130 IALRQIRLPKVKKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTENIYNT 189
           I LRQIRLP  K  G  +S  KEEPLIN F++EEIR+YI+PK++R+GK+NM+ TE +YNT
Sbjct: 130 IGLRQIRLP--KPEGIRNSTEKEEPLINKFLIEEIRQYITPKENRVGKINMFGTERVYNT 189

Query: 190 IGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRASKVYQKPYP 249
           IGHAC L + ELE YMDYD+G+YC DDWNLAQKLMLNGCDPLPRRRCLTRAS  YQKPYP
Sbjct: 190 IGHACALMKMELEKYMDYDVGAYCDDDWNLAQKLMLNGCDPLPRRRCLTRASMTYQKPYP 249

Query: 250 LNESLWKMPDDRNVRWSTYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKLKWVISNSSLA 309
           +NESLWK+PDDRNVRW  YQCRNF+CLSS NPK+GY KCSGCFEMEKEK KWV  NSSL 
Sbjct: 250 INESLWKLPDDRNVRWGNYQCRNFACLSSKNPKRGYTKCSGCFEMEKEKDKWV-KNSSLL 309

Query: 310 VDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFNEFIALRGL 369
           VDF I DVL +KP EIRIGLD+G+ TG+FAARMRE+NVTIVTTALNLGAPFNE IALRGL
Sbjct: 310 VDFMIEDVLRVKPSEIRIGLDYGVGTGTFAARMREKNVTIVTTALNLGAPFNEMIALRGL 369

Query: 370 FPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDRFFC 429
            PLY++LNQRLP FDNTMD+IHTTG MDGW+D+LL+DF+L+DWDRVLRPGGLLWIDRFFC
Sbjct: 370 IPLYISLNQRLPFFDNTMDMIHTTGLMDGWIDLLLMDFVLYDWDRVLRPGGLLWIDRFFC 429

Query: 430 DRKDLDDYMYMFLQFRYKKHKWSIAPKSRNEVYLSALLEKPPRAI 474
            +KDLDDYMYMFLQFRYKKHKW+I+PKS++EVYLSALLEKPPRAI
Sbjct: 430 KKKDLDDYMYMFLQFRYKKHKWAISPKSKDEVYLSALLEKPPRAI 463

BLAST of Chy8G156220 vs. TAIR 10
Match: AT4G01240.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 425.2 bits (1092), Expect = 6.7e-119
Identity = 215/459 (46.84%), Postives = 298/459 (64.92%), Query Frame = 0

Query: 25  LVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHVYEETFDANALSNRVEEVLDQLESL 84
           L  +++  +L KF     +   D  C Y  +     Y      + +   +E  + ++   
Sbjct: 207 LAFLISVATLAKFNYITPLRFSDRFCNYHGSFDGDNYN---IGSQMRKTIETAIFKIHQE 266

Query: 85  QEKLEFAVEEMGKNKQVLNNGKITMIEHKKYLEDEVIKPLYNAHIALRQIRLPKVKKNGQ 144
            + L+            +++   +M  H  +L D V+  + + H     + LP  ++   
Sbjct: 267 MDDLKALEANSSTPPSSVSSASGSMFRHVAFLAD-VLSLVQSVH-----MELPSFEERSV 326

Query: 145 NSSAMKEE--PLINNFVVEEIRKYISPKKSRIGKLNMYMTENIYNTIGHACVLYRKELED 204
               +K++      +F+ EEI+KYI  K +R+GK N       + +IGHAC   +K+LE+
Sbjct: 327 ADHPLKQQKGDPGEHFMREEIKKYIKIKPNRLGKQNFMGANGTFTSIGHACFAMKKDLEE 386

Query: 205 YMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRASKVYQKPYPLNESLWKMPDDRNV 264
           YMDYD+G  C DDW LAQKLM++GCDPLPRRRC +R  ++Y KP+P+NESLWK+PD+RNV
Sbjct: 387 YMDYDVGEICNDDWRLAQKLMVHGCDPLPRRRCFSRGPQLYHKPFPINESLWKLPDNRNV 446

Query: 265 RWSTYQCRNFSCLSSN-NPKKGYNKCSGCFEM-EKEKLKW-----VISNSSLAVDFAISD 324
           RW  Y+C+NF+CL+SN   +KG+ KC+ CF +   E  +W     +   ++   DF+I++
Sbjct: 447 RWGQYKCKNFACLASNTTARKGFFKCTDCFNLTHHESPRWLNRGEIDPETNQTADFSIAE 506

Query: 325 VLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFNEFIALRGLFPLYLTL 384
           VL IKPGEIRIGLDF I TG+FAARMRE+NVTIV+  +NLGAPFNE IALRGL PLYLT+
Sbjct: 507 VLEIKPGEIRIGLDFSIGTGTFAARMREQNVTIVSATINLGAPFNEMIALRGLVPLYLTV 566

Query: 385 NQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDRFFCDRKDLDD 444
           NQRLP FD+T+D+IHTT F+DGW+D++LLDF+LFDWDRVLRPGGLLWID FFC ++D+ D
Sbjct: 567 NQRLPFFDSTLDMIHTTRFLDGWIDLILLDFVLFDWDRVLRPGGLLWIDGFFCLKEDVSD 626

Query: 445 YMYMFLQFRYKKHKWSIAP---KSRNEVYLSALLEKPPR 472
           YM  F   RY+KHKW + P   K   EV+ SA+LEKPPR
Sbjct: 627 YMEAFKALRYRKHKWVVVPKKDKDDKEVFFSAVLEKPPR 656

BLAST of Chy8G156220 vs. TAIR 10
Match: AT3G27230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 5.9e-67
Identity = 133/315 (42.22%), Postives = 191/315 (60.63%), Query Frame = 0

Query: 159 VVEEIRKYISPKKSRIGKLNMYMTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNL 218
           +V E++ ++   +  +GK +      + +++GH+C      L  YM Y +   C DDW+L
Sbjct: 102 IVPELKLFLQRHQLPLGKDSRTGITEMVSSVGHSCGKSTDLLSQYMSYKVFDRCPDDWSL 161

Query: 219 AQKLMLNGCDPLPRRRCLTRASKVYQKPYPLNESLWKMPDDRNVRWSTYQCRNFSCLSSN 278
            QKL+L  C+PLPRRRCL +  +  Q      +SLW+   +++V WS   C++F CL   
Sbjct: 162 GQKLILRACEPLPRRRCLAKTVQ-KQDLSKSPDSLWRSVSNKSVNWSGLGCKSFDCLKG- 221

Query: 279 NPKKGYNKCSGCFEMEKEKLKWVISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFA 338
             KK   +C GCF++  EK ++V        DF I DVL +  G+IRIG D    +G+FA
Sbjct: 222 --KKLSKECVGCFDLGVEKDRFVKVKGK--NDFLIDDVLGLGSGKIRIGFDISGGSGTFA 281

Query: 339 ARMREENVTIVTTALNLGAPFNEFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGW 398
           ARM E+NVT++T  LN GAPF+EFIA RGLFPL+L+L+ R P  DN  DLIH +  +D  
Sbjct: 282 ARMAEKNVTVITNTLNNGAPFSEFIAARGLFPLFLSLDHRFPFLDNVFDLIHASSGLDVE 341

Query: 399 LDMLLLDFILFDWDRVLRPGGLLWIDRFFC-DRKDLDDYMYMFLQFRYKKHKWSIAPKSR 458
                L+F++FD DRVL+P GL W+D F+C + +   +   M  +F YKK KW I  K+ 
Sbjct: 342 GKAEKLEFLMFDLDRVLKPRGLFWLDNFYCANDEKKKELTRMIERFGYKKLKWVIGEKAD 401

Query: 459 NEVYLSALLEKPPRA 473
            +VYLSA+L+KP RA
Sbjct: 402 AQVYLSAVLQKPVRA 410

BLAST of Chy8G156220 vs. TAIR 10
Match: AT5G40830.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 250.0 bits (637), Expect = 3.8e-66
Identity = 133/322 (41.30%), Postives = 197/322 (61.18%), Query Frame = 0

Query: 159 VVEEIRKYISPKKSRIGKLNMYMTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNL 218
           V +E++ ++   +  +GK +      +  ++GH+C +    L  YM Y++   C DDW+L
Sbjct: 102 VPQELKLFLQQHQLPLGKDSRTGITQMVASVGHSCEMSLDLLSQYMSYNVFEKCPDDWSL 161

Query: 219 AQKLMLNGCDPLPRRRCLTRASKVYQKP----YPLNESLWKMPDDRNVRWSTYQCRNFSC 278
           AQKL+L  C+PLPRRRCL   +K   KP    +P  +SLW+   + +V WS   C++F C
Sbjct: 162 AQKLILRACEPLPRRRCL---AKTVHKPGLALFP--DSLWRPVGNSSVNWSGLGCKSFEC 221

Query: 279 LSSNNPKKGYNKCSGCFEM--EKEKLKWVISNSSLAVDFAISDVLAIKPGEIRIGLDFGI 338
           L     KK    C GCF++    EK ++V  N     DF I DVL +  G+IRIG D   
Sbjct: 222 LKG---KKLSRDCVGCFDLATSHEKDRFVKVNGK--TDFLIDDVLDLGDGKIRIGFDISS 281

Query: 339 STGSFAARMREENVTIVTTALNLGAPFNEFIALRGLFPLYLTLNQRLPLFDNTMDLIHTT 398
            +G+FAARM E+NV I++  LN+ APF+EFIA RG+FPL+++L+QRLP +DN  DLIH +
Sbjct: 282 GSGTFAARMAEKNVNIISNTLNIDAPFSEFIAARGIFPLFMSLDQRLPFYDNVFDLIHAS 341

Query: 399 GFMDGWLDML--LLDFILFDWDRVLRPGGLLWIDRFFCDRKDLDDYMYMFLQ-FRYKKHK 458
             +D  +      L+F++FD DR+L+PGGL W+D F+C   +    +   ++ F YKK K
Sbjct: 342 NGLDLAVSNKPEKLEFLMFDLDRILKPGGLFWLDNFYCGNDEKKRVLTRLIERFGYKKLK 401

Query: 459 WSIAPKSRNEVYLSALLEKPPR 472
           W +  K+  EV+LSA+L+KP R
Sbjct: 402 WVVGEKTDAEVFLSAVLQKPAR 413

BLAST of Chy8G156220 vs. TAIR 10
Match: AT5G40830.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 250.0 bits (637), Expect = 3.8e-66
Identity = 133/322 (41.30%), Postives = 197/322 (61.18%), Query Frame = 0

Query: 159 VVEEIRKYISPKKSRIGKLNMYMTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNL 218
           V +E++ ++   +  +GK +      +  ++GH+C +    L  YM Y++   C DDW+L
Sbjct: 102 VPQELKLFLQQHQLPLGKDSRTGITQMVASVGHSCEMSLDLLSQYMSYNVFEKCPDDWSL 161

Query: 219 AQKLMLNGCDPLPRRRCLTRASKVYQKP----YPLNESLWKMPDDRNVRWSTYQCRNFSC 278
           AQKL+L  C+PLPRRRCL   +K   KP    +P  +SLW+   + +V WS   C++F C
Sbjct: 162 AQKLILRACEPLPRRRCL---AKTVHKPGLALFP--DSLWRPVGNSSVNWSGLGCKSFEC 221

Query: 279 LSSNNPKKGYNKCSGCFEM--EKEKLKWVISNSSLAVDFAISDVLAIKPGEIRIGLDFGI 338
           L     KK    C GCF++    EK ++V  N     DF I DVL +  G+IRIG D   
Sbjct: 222 LKG---KKLSRDCVGCFDLATSHEKDRFVKVNGK--TDFLIDDVLDLGDGKIRIGFDISS 281

Query: 339 STGSFAARMREENVTIVTTALNLGAPFNEFIALRGLFPLYLTLNQRLPLFDNTMDLIHTT 398
            +G+FAARM E+NV I++  LN+ APF+EFIA RG+FPL+++L+QRLP +DN  DLIH +
Sbjct: 282 GSGTFAARMAEKNVNIISNTLNIDAPFSEFIAARGIFPLFMSLDQRLPFYDNVFDLIHAS 341

Query: 399 GFMDGWLDML--LLDFILFDWDRVLRPGGLLWIDRFFCDRKDLDDYMYMFLQ-FRYKKHK 458
             +D  +      L+F++FD DR+L+PGGL W+D F+C   +    +   ++ F YKK K
Sbjct: 342 NGLDLAVSNKPEKLEFLMFDLDRILKPGGLFWLDNFYCGNDEKKRVLTRLIERFGYKKLK 401

Query: 459 WSIAPKSRNEVYLSALLEKPPR 472
           W +  K+  EV+LSA+L+KP R
Sbjct: 402 WVVGEKTDAEVFLSAVLQKPAR 413

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RWB72.1e-5341.32Probable methyltransferase At1g29790 OS=Arabidopsis thaliana OX=3702 GN=CLSC5 PE... [more]
Match NameE-valueIdentityDescription
A0A5D3CEF48.7e-26896.62Putative Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BQC08.7e-26896.62uncharacterized protein LOC103492170 OS=Cucumis melo OX=3656 GN=LOC103492170 PE=... [more]
A0A6J1CY711.4e-24186.89uncharacterized protein LOC111015349 OS=Momordica charantia OX=3673 GN=LOC111015... [more]
A0A6J1I1D32.5e-23885.89uncharacterized protein LOC111468555 OS=Cucurbita maxima OX=3661 GN=LOC111468555... [more]
A0A6J1EZZ01.8e-23685.36uncharacterized protein LOC111440913 OS=Cucurbita moschata OX=3662 GN=LOC1114409... [more]
Match NameE-valueIdentityDescription
XP_011659893.10.098.52uncharacterized protein LOC101207467 [Cucumis sativus] >KAE8653367.1 hypothetica... [more]
XP_008450643.10.096.62PREDICTED: uncharacterized protein LOC103492170 [Cucumis melo] >KAA0050891.1 put... [more]
XP_038880508.10.093.38uncharacterized protein LOC120072172 [Benincasa hispida][more]
XP_022146046.15.76e-30686.89uncharacterized protein LOC111015349 [Momordica charantia][more]
XP_022969573.11.14e-30185.89uncharacterized protein LOC111468555 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G05390.13.1e-19370.11FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT4G01240.16.7e-11946.84S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G27230.15.9e-6742.22S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G40830.13.8e-6641.30S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G40830.23.8e-6641.30S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 67..101
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 292..467
e-value: 5.3E-6
score: 27.8
NoneNo IPR availablePANTHERPTHR44067S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN-RELATEDcoord: 8..472
NoneNo IPR availablePANTHERPTHR44067:SF9F22F7.17 PROTEINcoord: 8..472
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePFAMPF03141Methyltransf_29coord: 316..423
e-value: 4.4E-6
score: 25.5
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 309..452

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy8G156220.1Chy8G156220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0008168 methyltransferase activity