Chy8G156120 (gene) Cucumber (hystrix) v1

Overview
NameChy8G156120
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionIsoaspartyl peptidase/L-asparaginase
LocationchrH08: 17323386 .. 17326396 (+)
RNA-Seq ExpressionChy8G156120
SyntenyChy8G156120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATGGGCAATTGCTCTTCACGGCGGAGCCGGCGACATTCCCCTCTCATTACCATCGGACCGCCGCCAACCACGAGAGGAAACCCTCCGCCGTTGCCTGCAAATCGGCGTTCAAGCTCTCAAATCTCAGAAGTCTCCTTTGGATGTTGTTGAACTTGTGGTGAGAAATTTTTACTCTGTTTTTTTTTTTTTCGTTGGTGGACTCTGCGTTAAATATGAGCTTTAGAATCTAATGATTTAGTGTGTGGAATTTTCAAGTTTGTCTTGATTTAGTTCGAGATGGAGTGTTGAATTTGTAGTGATTTGGTCTCTAATGGGAGAAATTTTGATGAGTGATGGGGTTTTGTAATTAGTGACCAAATACAATTTTTCAAGGCTAGGATAATGGATTGTTACTGATTTTAATCCAGGGATTAATTTTTTTACATTTCTGAGGGTTTGATCAATGGGAATATTAAATTACAATGGCTTTGAACTAAATAAACTCTTCTGTTTTATGTCTTTTAGCTTTCTTTTCTTGGACTTTTGAAGTTTTTCCATGTTCTTTCTTTTCTGTTTCCTTTTCTTTCCGCCTGCTGAAACTCAGTTTATGTGGGTCCTTGCTTTTCTGCAAATGGGAAATTGTGTATGCTTTCATTTGGGGATTTGAGTTTTGATGTTATAACCTAGGCTAAGAAGCACATACACTGACATGCGAACAGACACTAATAAGCACAGACGCTGACATGCAAACAGACACTCATACAGCCACATGCCAATTTTTTTTAAAAAGTAGAACACTGCAAGTGGGACATGCTTTTTTTTAGCACAAGAATAAATATTTGTGTGCATACTTGACATATTGTGAAATATGCACTTATACATTTAACGAAAGAAAAAATTAGGAAGATAAAAGTGGAAGGACAAATAACAGAAATGAGAAAAGAAAAAAGAAAAAATATAGTAAGAGAAGTTGCCAGCAGACGTGAATCTTGAAGTTGAAGTCGTCGTTGTTGATAAAGTTAGAAAGAAGAATCGGGCAGCACAGTGTTTTTTTTAAAAAATATTCCTGAAAAATCCAGAATGTGAACTAAGTGACTTGAGTCTTAGGGTGAAGTGTTTTACTTTACAAAAAGTGTCCATAGTTTCATTTCAATGGGCTTAAAATGCCTTTTAAATGGGTTGTCATTGCCAATAGTAGGCTTTGTTTCATTTGTTTTAATTCCTTTTCTTTTTTATTTTTGTAGATGGTGTGTCCTATCGTGTTAGAAAGCGAAATAGTAATAATAAAATAATAGAGACACCAGTTGGCGTGTCGGACACATCACTGACGCTCGGCCATTTTAGGGGTGTTGGTCCTTTATAGGTTATAACCAAACATTTGCTTTTTAAGTTTTAACAAGAATGTTTTTTCAGCTCATTAATTAATGACTTCTTAGAGTGGAAATGGATTATTGATGGGAAATTAGAGAAACTACTGAAGGGGTGTTTGCCCTAAGGTTTAATCAGTCATTACTGAAATTTTGGGACGGAAAGGTTTAATTGTGATAGTTAAAAAAATTTGGATCGTATAAGTTCTTGGAATATATATTCTTAGTAAAAGATAACGACAGACTTAGGTGAGTTTCATAAGTCTATATATATTTTATCTTGTATAATTTATTTTGACTTGATATAAGATAGTTCCGTGTTGAATTTGTTCTTGTACAGGTACGAGAACTCGAAAATTGTCCACATTTCAATGCAGGTAGGGGGTCTGTCTTAACAACCAAGGGAACCGTAGAAATGGAAGCTAGCATCATGGACAGTACGAAGAGATGTGGAGCTGTTTCGGGTCTCACCACTGTCGTTAATCCCATATCTTTAGCTAGACTTGTTATGGAGAAAACTCCTCATATATATCTAGCATTTGATGGAGCTGAAGCTTTTGCAAGGGAACAGGTTGGGAATTTCTATCCCTCTTTCTATGTTACTGTAGAATTAATTCTCTTTTTATATATTATATATTATTGCGCAATTATAGATAGATTCATTGTGATTCATGACTGTTTTTTATTTGGATCAATTTTTCTGAAAAATGTCAAGGGAGTGGAGACCATTGATTCAAGCTACTTTATCACACCACAGAACATTGAAAGGCTACAACAAGCTAAAGAGGCAAACCGAGTGCAGGTTTTCTGCTTATCTTTTATCCCAATACATGAATTAACTGCTCTCTTTTTAAGCCTCTCTAAACTTACACGTATGTTATATTGTTAAGCTAGATCAATTGTGCCAACTTCTAGGCATTTTCACATGGTTTTAAATATTATTTCGATAAAAATATTGAGATTTTAATTTTATAAAAGTGTTAAGAGTGATATTTATAAGGAATTTAGGATAAAAAAGTGTAGAAGTGAGACAAATTGGAATTCTACACTTTTTAAATGAGTTAAATGTTATTAATACTACATTTACGTTTTGTTTATATACTTAATATTTTGTTGGCTTCTAAGGTGTAATAGAAATGTTGACATATCAGTGTTGACTTTCTGTTTTTAACCCTTGTAGATTGATTATACGCAACCACTTCCACAAGCCACAGAACAAGAAACTGAGAATCCAAATGGTGATAGTCAAATAGGAACTGTTGGATGTGTGGCTGTTGATAACCATGGAAATTTAGCTTCTGCAACTTCCACTGGTGGATTAGTGAACAAAATGGTTGGTAGGATTGGTGACACTCCGTTAATAGGAGCTGGCACATATGCTAACCATCTCTGTGCAATTTCTGCAACCGGAAAGGGTGAATCCATCATACACAGTACCGTGGCGAGGGATGTGGCAGCTCTAATGGAGTTCAAGGGCCTGTCTCTAGAAGAAGCAGCTGCCTCTGTTGTTGAAAGTGTTCCTAAAGGCAATGTCGGTTTGATCGCCGTATCTGCCTCTGGCGAAGTTGCGATGCCTTTTAATACGACTGGAATGTTTCGAGCTTGTGCAACTGAAGATGGTTATTCAGAGATTTCCATATGGTCTTCTAAACATGATTGA

mRNA sequence

ATGGGATGGGCAATTGCTCTTCACGGCGGAGCCGGCGACATTCCCCTCTCATTACCATCGGACCGCCGCCAACCACGAGAGGAAACCCTCCGCCGTTGCCTGCAAATCGGCGTTCAAGCTCTCAAATCTCAGAAGTCTCCTTTGGATGTTGTTGAACTTGTGGTACGAGAACTCGAAAATTGTCCACATTTCAATGCAGGTAGGGGGTCTGTCTTAACAACCAAGGGAACCGTAGAAATGGAAGCTAGCATCATGGACAGTACGAAGAGATGTGGAGCTGTTTCGGGTCTCACCACTGTCGTTAATCCCATATCTTTAGCTAGACTTGTTATGGAGAAAACTCCTCATATATATCTAGCATTTGATGGAGCTGAAGCTTTTGCAAGGGAACAGGGAGTGGAGACCATTGATTCAAGCTACTTTATCACACCACAGAACATTGAAAGGCTACAACAAGCTAAAGAGGCAAACCGAGTGCAGATTGATTATACGCAACCACTTCCACAAGCCACAGAACAAGAAACTGAGAATCCAAATGGTGATAGTCAAATAGGAACTGTTGGATGTGTGGCTGTTGATAACCATGGAAATTTAGCTTCTGCAACTTCCACTGGTGGATTAGTGAACAAAATGGTTGGTAGGATTGGTGACACTCCGTTAATAGGAGCTGGCACATATGCTAACCATCTCTGTGCAATTTCTGCAACCGGAAAGGGTGAATCCATCATACACAGTACCGTGGCGAGGGATGTGGCAGCTCTAATGGAGTTCAAGGGCCTGTCTCTAGAAGAAGCAGCTGCCTCTGTTGTTGAAAGTGTTCCTAAAGGCAATGTCGGTTTGATCGCCGTATCTGCCTCTGGCGAAGTTGCGATGCCTTTTAATACGACTGGAATGTTTCGAGCTTGTGCAACTGAAGATGGTTATTCAGAGATTTCCATATGGTCTTCTAAACATGATTGA

Coding sequence (CDS)

ATGGGATGGGCAATTGCTCTTCACGGCGGAGCCGGCGACATTCCCCTCTCATTACCATCGGACCGCCGCCAACCACGAGAGGAAACCCTCCGCCGTTGCCTGCAAATCGGCGTTCAAGCTCTCAAATCTCAGAAGTCTCCTTTGGATGTTGTTGAACTTGTGGTACGAGAACTCGAAAATTGTCCACATTTCAATGCAGGTAGGGGGTCTGTCTTAACAACCAAGGGAACCGTAGAAATGGAAGCTAGCATCATGGACAGTACGAAGAGATGTGGAGCTGTTTCGGGTCTCACCACTGTCGTTAATCCCATATCTTTAGCTAGACTTGTTATGGAGAAAACTCCTCATATATATCTAGCATTTGATGGAGCTGAAGCTTTTGCAAGGGAACAGGGAGTGGAGACCATTGATTCAAGCTACTTTATCACACCACAGAACATTGAAAGGCTACAACAAGCTAAAGAGGCAAACCGAGTGCAGATTGATTATACGCAACCACTTCCACAAGCCACAGAACAAGAAACTGAGAATCCAAATGGTGATAGTCAAATAGGAACTGTTGGATGTGTGGCTGTTGATAACCATGGAAATTTAGCTTCTGCAACTTCCACTGGTGGATTAGTGAACAAAATGGTTGGTAGGATTGGTGACACTCCGTTAATAGGAGCTGGCACATATGCTAACCATCTCTGTGCAATTTCTGCAACCGGAAAGGGTGAATCCATCATACACAGTACCGTGGCGAGGGATGTGGCAGCTCTAATGGAGTTCAAGGGCCTGTCTCTAGAAGAAGCAGCTGCCTCTGTTGTTGAAAGTGTTCCTAAAGGCAATGTCGGTTTGATCGCCGTATCTGCCTCTGGCGAAGTTGCGATGCCTTTTAATACGACTGGAATGTTTCGAGCTTGTGCAACTGAAGATGGTTATTCAGAGATTTCCATATGGTCTTCTAAACATGATTGA

Protein sequence

MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELENCPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPNGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGESIIHSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFRACATEDGYSEISIWSSKHD*
Homology
[Close]
BLAST of Chy8G156120 vs. ExPASy Swiss-Prot
Match: P30364 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.2e-135
Identity = 255/320 (79.69%), Postives = 281/320 (87.81%), Query Frame = 0 

Query: 2   GWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SLP +RRQPREE LR CLQIGV+ALKSQK PLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRQPREEGLRHCLQIGVEALKSQKPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTKGTVEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
            HFNAG GSVLT  GTVEMEASIMD  T +CGAVSGL+TV+NPISLARLVM+KTPHIYLA
Sbjct: 63  QHFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDKTPHIYLA 122

Query: 122 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQ---PLPQATEQETEN 181
           F GA+ FA++QGVET+DSS+FIT +N+ERL+ A EANRVQ+DY+Q   P P   + E E 
Sbjct: 123 FQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQDDAEKEL 182

Query: 182 P--NGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISA 241
           P  NGDSQIGTVGCVAVD+HGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SA
Sbjct: 183 PLANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGESIIHSTVARDVAALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFN 301
           TGKGE+II +TVARDVAALMEFKGLSL+EAA  VV E  PKG VGLIAVSA+GE+AMPFN
Sbjct: 243 TGKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEISIW 315
           TTGMFRA ATEDGYSEI+IW
Sbjct: 303 TTGMFRASATEDGYSEIAIW 322

[Close]
BLAST of Chy8G156120 vs. ExPASy Swiss-Prot
Match: Q9ZSD6 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 4.4e-135
Identity = 250/320 (78.12%), Postives = 280/320 (87.50%), Query Frame = 0 

Query: 2   GWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SLP +RR+PREE LR CLQIGV+ALK+QK PLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGVEALKAQKPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTKGTVEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
            HFNAG GSVLT  GTVEMEASIMD +T +CGAVSGL+TV+NPISLARLVM+KTPHIYLA
Sbjct: 63  EHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDKTPHIYLA 122

Query: 122 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQ-----PLPQATEQET 181
           F GA+ FA++QGVET+DSS+ IT +N+ERL+ A EANRVQ+DY+Q     P+    E+E 
Sbjct: 123 FQGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVKDDAEKEL 182

Query: 182 ENPNGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISA 241
              NGDSQIGTVGCVAVD+HGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SA
Sbjct: 183 PLTNGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGESIIHSTVARDVAALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFN 301
           TGKGE II +TVARDVAALMEFKGLSL+EAA  V+ E  PKG VGLIAVSA+GE+AMPFN
Sbjct: 243 TGKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEISIW 315
           TTGMFRACATEDGYSEI+IW
Sbjct: 303 TTGMFRACATEDGYSEIAIW 322

[Close]
BLAST of Chy8G156120 vs. ExPASy Swiss-Prot
Match: P50287 (Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08100 PE=1 SV=2)

HSP 1 Score: 481.1 bits (1237), Expect = 9.7e-135
Identity = 250/316 (79.11%), Postives = 274/316 (86.71%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELEN 60
           +GWAIALHGGAGDIP+ LP +RR PRE  LR CL +G+ ALKS K PLDV ELVVRELEN
Sbjct: 2   VGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELEN 61

Query: 61  CPHFNAGRGSVLTTKGTVEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120
            P FNAG+GSVLT +GTVEMEASIMD  TKRCGAVSGLTTVVNPISLARLVMEKTPHIYL
Sbjct: 62  HPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 121

Query: 121 AFDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPN 180
           AFD AEAFAR  GVET+DSS+FITP+NI RL+QAKE NRVQ+DYT P P+  +       
Sbjct: 122 AFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPKVPDN-----C 181

Query: 181 GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKG 240
           GDSQIGTVGCVAVD+ GNLASATSTGG VNKMVGRIGDTP+IGAGTYANHLCAISATGKG
Sbjct: 182 GDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKG 241

Query: 241 ESIIHSTVARDVAALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFNTTGM 300
           E II  TVARDVAALME+KGLSL EAAA VV +SVP+G+ GL+AVSA+GEV MPFNTTGM
Sbjct: 242 EDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGM 301

Query: 301 FRACATEDGYSEISIW 315
           FRACA+EDGYSEI+IW
Sbjct: 302 FRACASEDGYSEIAIW 312

[Close]
BLAST of Chy8G156120 vs. ExPASy Swiss-Prot
Match: P50288 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 1.8e-133
Identity = 247/320 (77.19%), Postives = 277/320 (86.56%), Query Frame = 0 

Query: 2   GWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SLP +RR+PREE LR CLQIG++ALK+Q  PLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGIEALKAQNPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTKGTVEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
            HFNAG GSVLT  GTVEMEASIMD +T +CGAVSGL TV+NPISLAR VM+KTPHI+LA
Sbjct: 63  QHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDKTPHIFLA 122

Query: 122 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQID-----YTQPLPQATEQET 181
           F GA+   ++QGVET+DSS+FIT +N+ERL+ A EANRVQ+D     YTQP+    E+E 
Sbjct: 123 FQGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQDDAEKEL 182

Query: 182 ENPNGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISA 241
              NGDSQIGTVGCVAVD+HGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SA
Sbjct: 183 PLANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGESIIHSTVARDVAALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFN 301
           TGKGE+II +TVARDVAALMEFKGLSL+EAA  VV E  PKG VGLIAVSA+GE+AMPFN
Sbjct: 243 TGKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEISIW 315
           TTGMFRACATEDGYSEI+IW
Sbjct: 303 TTGMFRACATEDGYSEIAIW 322

[Close]
BLAST of Chy8G156120 vs. ExPASy Swiss-Prot
Match: P30362 (Isoaspartyl peptidase/L-asparaginase (Fragment) OS=Lupinus arboreus OX=3872 PE=2 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 7.7e-124
Identity = 236/303 (77.89%), Postives = 264/303 (87.13%), Query Frame = 0 

Query: 19  PSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELENCPHFNAGRGSVLTTKGTV 78
           P +RR+PREE LR CLQIGV+ALK++KSPLDVVELVVRELEN  HFNAG GSVLT  GTV
Sbjct: 1   PPERRKPREEGLRHCLQIGVEALKARKSPLDVVELVVRELENNEHFNAGIGSVLTNSGTV 60

Query: 79  EMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDGAEAFAREQGVETID 138
           EMEASIMD  + +CGAVSGL+TV+NPISLARLVMEKTPH+YLAF GA+ FA++QGVET+D
Sbjct: 61  EMEASIMDGKSMKCGAVSGLSTVLNPISLARLVMEKTPHMYLAFQGAQDFAKQQGVETVD 120

Query: 139 SSYFITPQNIERLQQAKEANRVQID-----YTQPLPQATEQETENPNGDSQIGTVGCVAV 198
           SS+FIT +N+ERL+ A EANRVQID     YTQP+    E+E    NGDSQIGTVGCVAV
Sbjct: 121 SSHFITAENVERLKLAIEANRVQIDYSQYNYTQPVQDDAEKELPVANGDSQIGTVGCVAV 180

Query: 199 DNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGESIIHSTVARDVA 258
           D+ GNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SATGKGE+II +TVARDVA
Sbjct: 181 DSQGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSATGKGEAIIQATVARDVA 240

Query: 259 ALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFNTTGMFRACATEDGYSEI 315
           ALMEFKGLSL+EAA  VV E  PKG VGLIAVSA+GE+AMPFNTTGMFRACATEDG SEI
Sbjct: 241 ALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAGEIAMPFNTTGMFRACATEDGNSEI 300

[Close]
BLAST of Chy8G156120 vs. ExPASy TrEMBL
Match: A0A5A7UBC6 (Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00060 PE=4 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 6.1e-172
Identity = 313/319 (98.12%), Postives = 315/319 (98.75%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQK PLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPNG 180
           FDGAE FAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETEN NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 SIIHSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300
           +II STVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEISIWSSKHD 320
           ACATEDGYSEISIWSSKHD
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

[Close]
BLAST of Chy8G156120 vs. ExPASy TrEMBL
Match: A0A5D3CEE4 (Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003800 PE=4 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.3e-171
Identity = 312/319 (97.81%), Postives = 314/319 (98.43%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQK PLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPNG 180
           FDGAE FAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETEN NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DS IGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCAISATGKGE
Sbjct: 181 DSHIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 SIIHSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300
           +II STVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEISIWSSKHD 320
           ACATEDGYSEISIWSSKHD
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

[Close]
BLAST of Chy8G156120 vs. ExPASy TrEMBL
Match: A0A0A0LW19 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570100 PE=4 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.3e-171
Identity = 313/319 (98.12%), Postives = 314/319 (98.43%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQK PLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTK TVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPNG 180
           FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENP+G
Sbjct: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240

Query: 241 SIIHSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300
           SII STVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMP  TTGMFR
Sbjct: 241 SIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPLTTTGMFR 300

Query: 301 ACATEDGYSEISIWSSKHD 320
           ACATEDGYSEISIWSSKHD
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

[Close]
BLAST of Chy8G156120 vs. ExPASy TrEMBL
Match: A0A1S3BPR4 (isoaspartyl peptidase/L-asparaginase OS=Cucumis melo OX=3656 GN=LOC103492179 PE=4 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.3e-171
Identity = 312/319 (97.81%), Postives = 314/319 (98.43%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQK PLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPNG 180
           FDGAE FAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETEN NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DS IGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCAISATGKGE
Sbjct: 181 DSHIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 SIIHSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300
           +II STVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEISIWSSKHD 320
           ACATEDGYSEISIWSSKHD
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

[Close]
BLAST of Chy8G156120 vs. ExPASy TrEMBL
Match: A0A6J1F0F1 (isoaspartyl peptidase/L-asparaginase 1 OS=Cucurbita moschata OX=3662 GN=LOC111441019 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.2e-156
Identity = 289/316 (91.46%), Postives = 295/316 (93.35%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSL  DRRQPREETLRRCLQIGVQALK QK PLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTT GTVEMEASIMD  KRCGAVSGLTTVVN ISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPNG 180
           FDGAEAFAREQGVET D SYFITPQNIERL+QAKEAN VQIDYTQP PQAT QETE  NG
Sbjct: 121 FDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQETEIGNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYANHLCA+SATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240

Query: 241 SIIHSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300
           +II  TVARDVAALMEFK LSLEEAAA  VESVPKGNVGLIAVSASG+VAMPFNTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKDLSLEEAAACAVESVPKGNVGLIAVSASGDVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEISIWSS 317
           ACATEDGYSEI+IW S
Sbjct: 301 ACATEDGYSEIAIWPS 316

[Close]
BLAST of Chy8G156120 vs. NCBI nr
Match: KAA0050881.1 (isoaspartyl peptidase/L-asparaginase [Cucumis melo var. makuwa])

HSP 1 Score: 611 bits (1576), Expect = 3.74e-220
Identity = 313/319 (98.12%), Postives = 315/319 (98.75%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQK PLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPNG 180
           FDGAE FAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETEN NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 SIIHSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300
           +II STVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEISIWSSKHD 319
           ACATEDGYSEISIWSSKHD
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

[Close]
BLAST of Chy8G156120 vs. NCBI nr
Match: XP_004135623.1 (isoaspartyl peptidase/L-asparaginase 1 [Cucumis sativus] >KGN66085.1 hypothetical protein Csa_007301 [Cucumis sativus])

HSP 1 Score: 609 bits (1571), Expect = 2.17e-219
Identity = 313/319 (98.12%), Postives = 314/319 (98.43%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQK PLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTK TVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPNG 180
           FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENP+G
Sbjct: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240

Query: 241 SIIHSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300
           SII STVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMP  TTGMFR
Sbjct: 241 SIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPLTTTGMFR 300

Query: 301 ACATEDGYSEISIWSSKHD 319
           ACATEDGYSEISIWSSKHD
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

[Close]
BLAST of Chy8G156120 vs. NCBI nr
Match: XP_008450657.1 (PREDICTED: isoaspartyl peptidase/L-asparaginase [Cucumis melo] >TYK10233.1 isoaspartyl peptidase/L-asparaginase [Cucumis melo var. makuwa])

HSP 1 Score: 609 bits (1571), Expect = 2.17e-219
Identity = 312/319 (97.81%), Postives = 314/319 (98.43%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQK PLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPNG 180
           FDGAE FAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETEN NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DS IGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCAISATGKGE
Sbjct: 181 DSHIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 SIIHSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300
           +II STVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEISIWSSKHD 319
           ACATEDGYSEISIWSSKHD
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

[Close]
BLAST of Chy8G156120 vs. NCBI nr
Match: XP_038878291.1 (isoaspartyl peptidase/L-asparaginase isoform X1 [Benincasa hispida])

HSP 1 Score: 588 bits (1515), Expect = 7.42e-211
Identity = 299/319 (93.73%), Postives = 310/319 (97.18%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SLP +RRQPREETLRRCLQIGVQALKSQK PLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGT+EMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPNG 180
           FDGAEAFAREQGVET +SS+FITPQNIERL+QAKEANRVQIDYTQPLPQATEQETEN NG
Sbjct: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240

Query: 241 SIIHSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300
           +II  TVARDVAALMEFKGLSLEEA A VVESVPKGNVGL+AVSASGEVA+P+NTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300

Query: 301 ACATEDGYSEISIWSSKHD 319
           ACATEDGYSEI+IWSSK+D
Sbjct: 301 ACATEDGYSEIAIWSSKND 319

[Close]
BLAST of Chy8G156120 vs. NCBI nr
Match: XP_023530268.1 (isoaspartyl peptidase/L-asparaginase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563 bits (1451), Expect = 4.35e-201
Identity = 290/317 (91.48%), Postives = 296/317 (93.38%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSL  DRRQPREETLRRCLQIGVQALK QK P+DVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTT GTVEMEASIMD  KRCGAVSGLTTVVN ISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPNG 180
           FDGAEAFAREQGVET D SYFITPQNIERL+QAKEAN VQIDYTQP PQAT QETE  NG
Sbjct: 121 FDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATGQETEIGNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYANHLCA+SATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240

Query: 241 SIIHSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300
           +II  TVARDVAALMEFKGLSLEEAAA  VESVPKGNVGLIAVSASGEVAMPFNTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEISIWSSK 317
           ACATEDGYSEI+IW S 
Sbjct: 301 ACATEDGYSEIAIWPSN 317

[Close]
BLAST of Chy8G156120 vs. TAIR 10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 481.1 bits (1237), Expect = 6.9e-136
Identity = 250/316 (79.11%), Postives = 274/316 (86.71%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELEN 60
           +GWAIALHGGAGDIP+ LP +RR PRE  LR CL +G+ ALKS K PLDV ELVVRELEN
Sbjct: 2   VGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELEN 61

Query: 61  CPHFNAGRGSVLTTKGTVEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120
            P FNAG+GSVLT +GTVEMEASIMD  TKRCGAVSGLTTVVNPISLARLVMEKTPHIYL
Sbjct: 62  HPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 121

Query: 121 AFDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPN 180
           AFD AEAFAR  GVET+DSS+FITP+NI RL+QAKE NRVQ+DYT P P+  +       
Sbjct: 122 AFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPKVPDN-----C 181

Query: 181 GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKG 240
           GDSQIGTVGCVAVD+ GNLASATSTGG VNKMVGRIGDTP+IGAGTYANHLCAISATGKG
Sbjct: 182 GDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKG 241

Query: 241 ESIIHSTVARDVAALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFNTTGM 300
           E II  TVARDVAALME+KGLSL EAAA VV +SVP+G+ GL+AVSA+GEV MPFNTTGM
Sbjct: 242 EDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGM 301

Query: 301 FRACATEDGYSEISIW 315
           FRACA+EDGYSEI+IW
Sbjct: 302 FRACASEDGYSEIAIW 312

[Close]
BLAST of Chy8G156120 vs. TAIR 10
Match: AT5G08100.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 361.7 bits (927), Expect = 6.1e-100
Identity = 192/237 (81.01%), Postives = 209/237 (88.19%), Query Frame = 0 

Query: 80  MEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDGAEAFAREQGVETIDS 139
           MEASIMD  TKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFD AEAFAR  GVET+DS
Sbjct: 1   MEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDS 60

Query: 140 SYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPNGDSQIGTVGCVAVDNHGNL 199
           S+FITP+NI RL+QAKE NRVQ+DYT P P+  +       GDSQIGTVGCVAVD+ GNL
Sbjct: 61  SHFITPENIARLKQAKEFNRVQLDYTVPSPKVPDN-----CGDSQIGTVGCVAVDSAGNL 120

Query: 200 ASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGESIIHSTVARDVAALMEFK 259
           ASATSTGG VNKMVGRIGDTP+IGAGTYANHLCAISATGKGE II  TVARDVAALME+K
Sbjct: 121 ASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKGEDIIRGTVARDVAALMEYK 180

Query: 260 GLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFNTTGMFRACATEDGYSEISIW 315
           GLSL EAAA VV +SVP+G+ GL+AVSA+GEV MPFNTTGMFRACA+EDGYSEI+IW
Sbjct: 181 GLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGMFRACASEDGYSEIAIW 232

[Close]
BLAST of Chy8G156120 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 357.5 bits (916), Expect = 1.2e-98
Identity = 190/323 (58.82%), Postives = 243/323 (75.23%), Query Frame = 0 

Query: 2   GWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELENC 61
           GWAIA+HGGAG  P +LP++R++  ++ L RCL +G+ AL+S  S +DVVELV+RELE  
Sbjct: 3   GWAIAVHGGAGIDP-NLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELETD 62

Query: 62  PHFNAGRGSVLTTKGTVEMEASIMDSTK-RCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
           P FN+GRGS LT KGTVEMEASIMD TK RCGAVSG+TTV NPISLARLVM+K+PH YLA
Sbjct: 63  PLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYLA 122

Query: 122 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDY-TQPLPQATEQETENPN 181
           F GAE FAR+QGVE +D+ YF+T  N+  L+ AKEAN +  DY   P+  A    T++P 
Sbjct: 123 FSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSPI 182

Query: 182 GDSQI-------GTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCA 241
             + +        TVGCV VD  G+ A+ TSTGGL+NKM+GRIGD+PLIGAGTYA+  C 
Sbjct: 183 QMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFCG 242

Query: 242 ISATGKGESIIHSTVARDVAALMEFKGLSLEEAAASVVE-SVPKGNVGLIAVSASGEVAM 301
           +S TG+GE+II +T+ARDV+A+ME+KGL+L+EA   V++  + +G  GLIAVS  GEV  
Sbjct: 243 VSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVVC 302

Query: 302 PFNTTGMFRACATEDGYSEISIW 315
            FN+ GMFR CATEDG+ E++IW
Sbjct: 303 GFNSNGMFRGCATEDGFMEVAIW 324

[Close]
BLAST of Chy8G156120 vs. TAIR 10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 85.1 bits (209), Expect = 1.1e-16
Identity = 87/335 (25.97%), Postives = 140/335 (41.79%), Query Frame = 0 

Query: 5   IALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELENCPHF 64
           +A+H GAG       ++ +  R    R CL       +     +D V   ++ LE+ P  
Sbjct: 15  VAVHVGAG---YHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 65  NAGRGSVLTTKGTVEMEASIMD-STKRCGAVSGLTTVVNPISLARLVME----------K 124
           NAGRGS LT  G VE +AS+MD  +   G V  +  V N I +A L+++          +
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKEQITGSTLLGR 134

Query: 125 TPHIYLAFDGAEAFAREQ-----GVETIDSSYFITPQNIERLQQAK----EANRVQIDYT 184
            P + L  +GA  + + +     G  T    + +T +   + ++ K    E     I   
Sbjct: 135 IPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSA 194

Query: 185 QPLPQATE-QETENPN--------GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRI 244
           +  P+ TE  ET   N         D  + TVG + VDN G++A  +S+GG+  K+ GR+
Sbjct: 195 EEHPRGTENNETCEENVSSCAAADEDKIMDTVGVICVDNEGHIACGSSSGGIAMKISGRV 254

Query: 245 GDTPLIGAGTYAN--------HLCAISATGKGESIIHSTVARDVAALMEFKGLSLEEAAA 293
           G     G+G +A+         L     +G GE ++    AR+    +         AA 
Sbjct: 255 GLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAM 314

[Close]
BLAST of Chy8G156120 vs. TAIR 10
Match: AT4G00590.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 78.6 bits (192), Expect = 1.0e-14
Identity = 85/326 (26.07%), Postives = 137/326 (42.02%), Query Frame = 0 

Query: 5   IALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKSPLDVVELVVRELENCPHF 64
           +A+H GAG       ++ +  R    R CL       +     +D V   ++ LE+ P  
Sbjct: 15  VAVHVGAG---YHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 65  NAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPIS--LARLVMEKTPHIYLAFD 124
           NAGRGS LT  G VE +AS+MD     G   G+  V + +S  L++L++       L  +
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDS--GMFGGVGAVPDQVSEMLSKLLL----CWLLVGE 134

Query: 125 GAEAFAREQ-----GVETIDSSYFITPQNIERLQQAK----EANRVQIDYTQPLPQATE- 184
           GA  + + +     G  T    + +T +   + ++ K    E     I   +  P+ TE 
Sbjct: 135 GARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSAEEHPRGTEN 194

Query: 185 QETENPN--------GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAG 244
            ET   N         D  + TVG + VDN G++A  +S+GG+  K+ GR+G     G+G
Sbjct: 195 NETCEENVSSCAAADEDKIMDTVGVICVDNEGHIACGSSSGGIAMKISGRVGLAATYGSG 254

Query: 245 TYAN--------HLCAISATGKGESIIHSTVARDVAALMEFKGLSLEEAAASVVESV--- 293
            +A+         L     +G GE ++    AR+    +         AA  V+ SV   
Sbjct: 255 CWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAMKVLRSVMHQ 314

The following BLAST results are available for this feature:
BLAST of Chy8G156120 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P303641.2e-13579.69Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1[more]
Q9ZSD64.4e-13578.13Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1[more]
P502879.7e-13579.11Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08... [more]
P502881.8e-13377.19Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1[more]
P303627.7e-12477.89Isoaspartyl peptidase/L-asparaginase (Fragment) OS=Lupinus arboreus OX=3872 PE=2... [more]
BLAST of Chy8G156120 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7UBC66.1e-17298.12Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5D3CEE42.3e-17197.81Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A0A0LW192.3e-17198.12Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570100 PE=4 SV=1[more]
A0A1S3BPR42.3e-17197.81isoaspartyl peptidase/L-asparaginase OS=Cucumis melo OX=3656 GN=LOC103492179 PE=... [more]
A0A6J1F0F11.2e-15691.46isoaspartyl peptidase/L-asparaginase 1 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
BLAST of Chy8G156120 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0050881.13.74e-22098.12isoaspartyl peptidase/L-asparaginase [Cucumis melo var. makuwa][more]
XP_004135623.12.17e-21998.12isoaspartyl peptidase/L-asparaginase 1 [Cucumis sativus] >KGN66085.1 hypothetica... [more]
XP_008450657.12.17e-21997.81PREDICTED: isoaspartyl peptidase/L-asparaginase [Cucumis melo] >TYK10233.1 isoas... [more]
XP_038878291.17.42e-21193.73isoaspartyl peptidase/L-asparaginase isoform X1 [Benincasa hispida][more]
XP_023530268.14.35e-20191.48isoaspartyl peptidase/L-asparaginase 1 [Cucurbita pepo subsp. pepo][more]
BLAST of Chy8G156120 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G08100.16.9e-13679.11N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G08100.26.1e-10081.01N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT3G16150.11.2e-9858.82N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G00590.11.1e-1625.97N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G00590.21.0e-1426.07N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000246Peptidase T2, asparaginase 2PFAMPF01112Asparaginase_2coord: 3..314
e-value: 4.8E-113
score: 377.3
IPR000246Peptidase T2, asparaginase 2PANTHERPTHR10188L-ASPARAGINASEcoord: 1..314
NoneNo IPR availableGENE3D3.60.20.30(Glycosyl)asparaginasecoord: 186..317
e-value: 2.0E-44
score: 152.9
NoneNo IPR availablePIRSRPIRSR600246-2PIRSR600246-2coord: 2..318
e-value: 7.0E-108
score: 358.4
NoneNo IPR availablePIRSRPIRSR600246-1PIRSR600246-1coord: 2..318
e-value: 7.0E-108
score: 358.4
NoneNo IPR availablePIRSRPIRSR600246-3PIRSR600246-3coord: 2..318
e-value: 7.0E-108
score: 358.4
NoneNo IPR availablePANTHERPTHR10188:SF33ISOASPARTYL PEPTIDASE/L-ASPARAGINASEcoord: 1..314
NoneNo IPR availableCDDcd04701Asparaginase_2coord: 4..307
e-value: 9.38358E-137
score: 386.432
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 3..313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy8G156120.1Chy8G156120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016540 protein autoprocessing
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004067 asparaginase activity
molecular_function GO:0008798 beta-aspartyl-peptidase activity
molecular_function GO:0016787 hydrolase activity