Chy8G155320 (gene) Cucumber (hystrix) v1

BLAST of Chy8G155320 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 3.9e-95
Identity = 180/348 (51.72%), Postives = 235/348 (67.53%), Query Frame = 0

Query: 11  GSAGGFYGNLRHHHHRHRHRSHLSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPG 70
           G   G  G+ ++HH       +  R TKLFVFGDSY DTGNI   FSS+ +FPYGITFPG
Sbjct: 22  GEINGVEGSNQNHH------LYPFRPTKLFVFGDSYADTGNIKKAFSSSWKFPYGITFPG 81

Query: 71  KPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPL 130
           KP+GRFSDGRV TDF AK +G+KSPIP+  +   G++RL + G+NFA+GGTGVFNT  PL
Sbjct: 82  KPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRL-QYGMNFAYGGTGVFNTQTPL 141

Query: 131 PNMTTQIDLFEQLRHDESGLSNRDVHLSLALVSVSGNDYSFYLATNGSTQGLKPFINSVV 190
           PNMTTQID+F+ +          ++  S+ALVSV+GNDYS ++A N        FI  VV
Sbjct: 142 PNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIALNRPASEFPAFIKQVV 201

Query: 191 NQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLKQAV 250
           +Q  ++LRRI  LGVKKI V  L PLGCLP FT   S+++CN+T N+ V  HN LL+Q V
Sbjct: 202 DQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNETQNALVNLHNNLLQQVV 261

Query: 251 DKLNKQITKQQHSSSSSSSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGLLKFKTPLKPC 310
            KLN + TKQ          S   ILD+Y+AFL++ + + S  GS R     F++PLKPC
Sbjct: 262 AKLNNE-TKQ----------STFIILDLYNAFLTVFKNKGSNPGSTR-----FESPLKPC 321

Query: 311 CFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGWALAFS 359
           C GVS  + CGSVDE+G +K+++C++PK+AFFWD +HPT+ GW   +S
Sbjct: 322 CVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYS 346

BLAST of Chy8G155320 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.4e-83
Identity = 160/348 (45.98%), Postives = 221/348 (63.51%), Query Frame = 0

Query: 12  SAGGFYGNLRHHHHRHRHRSHLSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGK 71
           S   F+      + +H +   L R  +LFVFGDSY DTGNI    S + + PYGITFP K
Sbjct: 14  SFSSFFLGAESDYPQHSNLKRL-RPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQK 73

Query: 72  PSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTL-VPL 131
           PSGRFSDGRV TDF A++LG+KSPIP++ +   G+ERL   G+N+A+GGTGVF T   PL
Sbjct: 74  PSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLL-YGMNYAYGGTGVFKTKDNPL 133

Query: 132 PNMTTQIDLFEQLRHDESGLSNRDVHLSLALVSVSGNDYSFYLATNGSTQGLKPFINSVV 191
           PNMTTQID F+++    +  S  D+  SLALVSV+GNDY+ +LA       L  F+  VV
Sbjct: 134 PNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPLTELPAFMKQVV 193

Query: 192 NQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLKQAV 251
           +QI ++  RI +LGV KIV+  + PLGCLP  T   S+++CN T N+    HN+LL +A+
Sbjct: 194 DQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLHKAI 253

Query: 252 DKLNKQITKQQHSSSSSSSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGLLKFKTPLKPC 311
            +LN +           +  S   +LD Y+AFL++ + +    G  R G      PLKPC
Sbjct: 254 ARLNNE-----------TKPSTFVVLDHYNAFLTVFKNKGPEPGVSRFG-----NPLKPC 313

Query: 312 CFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGWALAFS 359
           C GV+S ++C +VDE+G +K+++C DPK+AFFWD  HP++ GW   +S
Sbjct: 314 CVGVNSSYDCSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYS 343

BLAST of Chy8G155320 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 240.0 bits (611), Expect = 4.3e-62
Identity = 135/331 (40.79%), Postives = 195/331 (58.91%), Query Frame = 0

Query: 31  SHLSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHL 90
           S  ++  KLFVFG+SY DTGN + P + + + PYGITFPGKPSGR+SDG   TDF AK L
Sbjct: 27  SQANKNPKLFVFGNSYADTGN-MKPTALSWKLPYGITFPGKPSGRYSDGLTATDFLAKQL 86

Query: 91  GVKSPIPFSIRSEVGEERLK-ESGINFAFGGTGVFNTLVP-LPNMTTQIDLFEQLRHDES 150
           G K  +P+  R+  G++++K   G+NFAFGG+ VF++ V   PN++TQ+     L     
Sbjct: 87  GAK--LPYLWRTH-GKKKVKLNRGMNFAFGGSEVFDSPVDRSPNISTQVGFLVNLALARR 146

Query: 151 GLS-NRDVHLSLALVSVSGNDYSFYLATNGSTQGLKPFINSVVNQIMIDLRRIRRLGVKK 210
             + + D+  S AL+S SG DY  ++  N +      F+  +V  I   L  +  L  K 
Sbjct: 147 VYTIDGDLASSYALLSYSGTDYYGFIDQNPNMAAYPAFVEFIVEDIQYSLGIMNGLKFKN 206

Query: 211 IVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLKQAVDKLNKQITKQQHSSSSS 270
           I VT L PLGCLP  T   S++ CN++ +  V+ HN  LK+AV KLNK+           
Sbjct: 207 IAVTSLHPLGCLPRVTVASSFRSCNESYSDLVRLHNESLKKAVAKLNKE-------DKFR 266

Query: 271 SSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGLLKFKTPLKPCCFGVSSGFECGSVDEQG 330
           +   +  I+D++ AF++I++         + G  +FK+PLKPCC G     +C  +D +G
Sbjct: 267 TKGDRFVIVDLHKAFMTILE---------KKGNKRFKSPLKPCCEG-----DCARMDMKG 326

Query: 331 NRKFVLCNDPKSAFFWDSVHPTQTGWALAFS 359
            +K+ LCNDPKSAFFWD ++PTQ GW   +S
Sbjct: 327 AKKYTLCNDPKSAFFWDEINPTQEGWRSIYS 332

BLAST of Chy8G155320 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 8.1e-61
Identity = 131/327 (40.06%), Postives = 192/327 (58.72%), Query Frame = 0

Query: 38  KLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIP 97
           KLFVFGDSY DTGN     + A   PYGITFPGKPSGR+ DG + TDF  K LG +SP  
Sbjct: 14  KLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAESPYL 73

Query: 98  FSIRSEVGEERLKESGINFAFGGTGVFNTL--VPLPNMTTQIDLFEQLRHDESGLSNRDV 157
           +      G ++  + G+NFAFGG+ + ++    P PN+T Q++    L    +G    D+
Sbjct: 74  YRTH---GRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDL--VLAGRVYGDI 133

Query: 158 HLS-LALVSVSGNDYSFYLATNGSTQGLKPFINSVVNQIMIDLRRIRRLGVKKIVVTGLG 217
             S ++L+S +G DY +Y+  N    GLK  +  VV+ + +++  +  L  KKI VT L 
Sbjct: 134 TPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIAVTSLQ 193

Query: 218 PLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLKQAVDKLNKQ---ITKQQHSSSSSSSSS 277
           P+GCLP +T+  S+K CN++ ++ V+ HN LLK+ V KLN+Q   + K+QH         
Sbjct: 194 PIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQH--------- 253

Query: 278 KIFILDVYDAFLSIIQGRDSGSGSGRVGLLKFKTPLKPCCFGVSSGFECGSVDEQGNRKF 337
             FI+D+++AF+++++ +         G  +FK P+K CC G      CG     G + +
Sbjct: 254 -FFIIDIHNAFMTVMKNK---------GSKRFKNPMKSCCEGY-----CGR-SSDGGKLY 309

Query: 338 VLCNDPKSAFFWDSVHPTQTGWALAFS 359
            LC+DPKS FFWD+VHPTQ GW   +S
Sbjct: 314 TLCDDPKSFFFWDAVHPTQEGWRSIYS 309

BLAST of Chy8G155320 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 235.0 bits (598), Expect = 1.4e-60
Identity = 135/324 (41.67%), Postives = 185/324 (57.10%), Query Frame = 0

Query: 37  TKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPI 96
           TKLFVFGDSY DTGN  F    + +FP GITFPG P+GRFSDGRV TD+ AK++GV++PI
Sbjct: 46  TKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRTPI 105

Query: 97  PFSIRSEVGEERLK-ESGINFAFGGTGVFNTLVPL-PNMTTQIDLFEQLRHDESGLSNRD 156
            +    + G  RL  + G+NFA+GG G F T+  L P  + QID FEQL    +  S  D
Sbjct: 106 TYK-WGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLM-RNVYSPAD 165

Query: 157 VHLSLALVSVSGNDYSFYLATNGSTQGLKPFINSVVNQIMIDLRRIRRLGVKKIVVTGLG 216
           ++ S+A  S+ GNDY  Y   NGS +G       VV QI++D++RI+ LGV+K++V    
Sbjct: 166 LNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVALSP 225

Query: 217 PLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLKQAVDKLNKQITKQQHSSSSSSSSSKIF 276
           P  CLP    P   K C+ T ++    HN LL++ + KLN            +++     
Sbjct: 226 PQKCLPKLVTP---KGCD-TNDTSTYLHNSLLRKGLIKLN--------DKEINNNDKSFM 285

Query: 277 ILDVYDAFLSIIQGRDSGSGSGRVGLLKFKTPLKPCCFGVSSGFECGSVDEQGNRKFVLC 336
            LD+Y+AF++I + +      G  G+  F  P K CC     G  CG     G + + LC
Sbjct: 286 TLDLYNAFVTIFKNK------GVSGVSTFPDPFKACC-ATKRGTFCGDRSLSGKKLYTLC 345

Query: 337 NDPKSAFFWDSVHPTQTGWALAFS 359
           +DPKS FFWD+VH +  GW   FS
Sbjct: 346 DDPKSFFFWDNVHISDQGWRSVFS 348

BLAST of Chy8G155320 vs. ExPASy TrEMBL
Match: A0A1S3CJ29 (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500972 PE=3 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.4e-180
Identity = 322/363 (88.71%), Postives = 335/363 (92.29%), Query Frame = 0

Query: 7   KTGQGSAGGFYGNLRHHHHRHRHRSHLSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGI 66
           KTGQGSAGG+Y N      RH HRSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGI
Sbjct: 1   KTGQGSAGGYYSN------RH-HRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGI 60

Query: 67  TFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNT 126
           TFPGKPSGRFSDGRVLTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T
Sbjct: 61  TFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDT 120

Query: 127 LVPLPNMTTQIDLFEQLRHDESGLSNRDVHLSLALVSVSGNDYSFYLATNGSTQGLKPFI 186
            VPLPNMTTQIDLFEQLRH ESGLSNRDVHLS+ALVSVSGNDYSFYLATNGS QGLKPFI
Sbjct: 121 SVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFI 180

Query: 187 NSVVNQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQFHNFLL 246
           NSVVNQ+MIDL+RI+RLGVKKIVVTGLGPLGCLPIFTAPFS+KQCNQTINSFVQFHNFLL
Sbjct: 181 NSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLL 240

Query: 247 KQAVDKLNKQITKQQH-----SSSSSSSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGLL 306
           KQAVDKLNKQIT QQH     SSSSSSSSSKIFILDVYDAFLS+IQGR    GSGRVGLL
Sbjct: 241 KQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGR----GSGRVGLL 300

Query: 307 KFKTPLKPCCFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGWALAFSSFT 365
           KFKTPLKPCCFGVSSGF CGSVDE+GN+KFVLCNDPKSAFFWDSVHPTQTGW+LAFSSFT
Sbjct: 301 KFKTPLKPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFT 352

BLAST of Chy8G155320 vs. ExPASy TrEMBL
Match: A0A5A7SLZ4 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001230 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.6e-179
Identity = 320/362 (88.40%), Postives = 334/362 (92.27%), Query Frame = 0

Query: 8   TGQGSAGGFYGNLRHHHHRHRHRSHLSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGIT 67
           +GQGSAGG+Y N      RH HRSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGIT
Sbjct: 20  SGQGSAGGYYSN------RH-HRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGIT 79

Query: 68  FPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTL 127
           FPGKPSGRFSDGRVLTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T 
Sbjct: 80  FPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTS 139

Query: 128 VPLPNMTTQIDLFEQLRHDESGLSNRDVHLSLALVSVSGNDYSFYLATNGSTQGLKPFIN 187
           VPLPNMTTQIDLFEQLRH ESGLSNRDVHLS+ALVSVSGNDYSFYLATNGS QGLKPFIN
Sbjct: 140 VPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFIN 199

Query: 188 SVVNQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLK 247
           SVVNQ+MIDL+RI+RLGVKKIVVTGLGPLGCLPIFTAPFS+KQCNQTINSFVQFHNFLLK
Sbjct: 200 SVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLK 259

Query: 248 QAVDKLNKQITKQQH-----SSSSSSSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGLLK 307
           QAVDKLNKQIT QQH     SSSSSSSSSKIFILDVYDAFLS+IQGR    GSGRVGLLK
Sbjct: 260 QAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGR----GSGRVGLLK 319

Query: 308 FKTPLKPCCFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGWALAFSSFTS 365
           FKTPLKPCCFGVSSGF CGSVDE+GN+KFVLCNDPKSAFFWDSVHPTQTGW+LAFSSFTS
Sbjct: 320 FKTPLKPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTS 370

BLAST of Chy8G155320 vs. ExPASy TrEMBL
Match: A0A5D3C686 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001210 PE=3 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 1.7e-178
Identity = 319/360 (88.61%), Postives = 332/360 (92.22%), Query Frame = 0

Query: 10  QGSAGGFYGNLRHHHHRHRHRSHLSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFP 69
           QGSAGG+Y N      RH HRSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGITFP
Sbjct: 5   QGSAGGYYSN------RH-HRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFP 64

Query: 70  GKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVP 129
           GKPSGRFSDGRVLTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T VP
Sbjct: 65  GKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVP 124

Query: 130 LPNMTTQIDLFEQLRHDESGLSNRDVHLSLALVSVSGNDYSFYLATNGSTQGLKPFINSV 189
           LPNMTTQIDLFEQLRH ESGLSNRDVHLS+ALVSVSGNDYSFYLATNGS QGLKPFINSV
Sbjct: 125 LPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSV 184

Query: 190 VNQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLKQA 249
           VNQ+MIDL+RI+RLGVKKIVVTGLGPLGCLPIFTAPFS+KQCNQTINSFVQFHNFLLKQA
Sbjct: 185 VNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQA 244

Query: 250 VDKLNKQITKQQH-----SSSSSSSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGLLKFK 309
           VDKLNKQIT QQH     SSSSSSSSSKIFILDVYDAFLS+IQGR    GSGRVGLLKFK
Sbjct: 245 VDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGR----GSGRVGLLKFK 304

Query: 310 TPLKPCCFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGWALAFSSFTSFL 365
           TPLKPCCFGVSSGF CGSVDE+GN+KFVLCNDPKSAFFWDSVHPTQTGW+LAFSSFTS L
Sbjct: 305 TPLKPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL 353

BLAST of Chy8G155320 vs. ExPASy TrEMBL
Match: A0A6J1ECT1 (GDSL esterase/lipase At5g03610-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.8e-135
Identity = 241/356 (67.70%), Postives = 278/356 (78.09%), Query Frame = 0

Query: 8   TGQGSAGGFYGNLRHHHHRHRHRSHLSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGIT 67
           +G+GSAGG Y    HHHHR RH  H  R TKLFVFGDSY DTGNILFP S A QFPYGIT
Sbjct: 19  SGRGSAGGVY---NHHHHRRRHLRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGIT 78

Query: 68  FPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTL 127
           FPGKP GRFSDGRVLTDFAA+H+G+KSPIPFS+R+ VG  R KE+G+NFAFGGTGVF+T 
Sbjct: 79  FPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTE 138

Query: 128 VPLPNMTTQIDLFEQLRHDESGLSNRDVHLSLALVSVSGNDYSFYLATNGSTQGLKPFIN 187
            PLPNMTTQID F+QLRH +S  +N D   S+ALVS SGNDY+FY A N S QGLKPFI 
Sbjct: 139 YPLPNMTTQIDFFQQLRHGDSIFTNTDAQSSVALVSASGNDYAFYFAMNSSRQGLKPFIM 198

Query: 188 SVVNQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLK 247
           SVV+QI+++L+RI  LGVKKI VTGLGPLGCLP+FTAP SYKQCN+TINS VQFHN LLK
Sbjct: 199 SVVSQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKQCNETINSLVQFHNLLLK 258

Query: 248 QAVDKLNKQITKQQHSSSSSSSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGLLKFKTPL 307
           +AV KLN++          +  SSK+F++DVY AF+SII+GR       R    +FKTPL
Sbjct: 259 EAVKKLNQE----------TKHSSKVFVVDVYGAFMSIIEGRK------RYNNGEFKTPL 318

Query: 308 KPCCFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGWALAFSSFTSF 364
           KPCC+GV SGF CGSVD +G + FVLC D K+AFFWDSVHPTQ GWA A S+F SF
Sbjct: 319 KPCCYGVGSGFSCGSVDGEGKKMFVLCEDQKAAFFWDSVHPTQQGWAAAISAFKSF 355

BLAST of Chy8G155320 vs. ExPASy TrEMBL
Match: A0A6J1EIS2 (GDSL esterase/lipase At5g03610-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.8e-135
Identity = 241/356 (67.70%), Postives = 278/356 (78.09%), Query Frame = 0

Query: 8   TGQGSAGGFYGNLRHHHHRHRHRSHLSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGIT 67
           +G+GSAGG Y    HHHHR RH  H  R TKLFVFGDSY DTGNILFP S A QFPYGIT
Sbjct: 20  SGRGSAGGVY---NHHHHRRRHLRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGIT 79

Query: 68  FPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTL 127
           FPGKP GRFSDGRVLTDFAA+H+G+KSPIPFS+R+ VG  R KE+G+NFAFGGTGVF+T 
Sbjct: 80  FPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTE 139

Query: 128 VPLPNMTTQIDLFEQLRHDESGLSNRDVHLSLALVSVSGNDYSFYLATNGSTQGLKPFIN 187
            PLPNMTTQID F+QLRH +S  +N D   S+ALVS SGNDY+FY A N S QGLKPFI 
Sbjct: 140 YPLPNMTTQIDFFQQLRHGDSIFTNTDAQSSVALVSASGNDYAFYFAMNSSRQGLKPFIM 199

Query: 188 SVVNQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLK 247
           SVV+QI+++L+RI  LGVKKI VTGLGPLGCLP+FTAP SYKQCN+TINS VQFHN LLK
Sbjct: 200 SVVSQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKQCNETINSLVQFHNLLLK 259

Query: 248 QAVDKLNKQITKQQHSSSSSSSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGLLKFKTPL 307
           +AV KLN++          +  SSK+F++DVY AF+SII+GR       R    +FKTPL
Sbjct: 260 EAVKKLNQE----------TKHSSKVFVVDVYGAFMSIIEGRK------RYNNGEFKTPL 319

Query: 308 KPCCFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGWALAFSSFTSF 364
           KPCC+GV SGF CGSVD +G + FVLC D K+AFFWDSVHPTQ GWA A S+F SF
Sbjct: 320 KPCCYGVGSGFSCGSVDGEGKKMFVLCEDQKAAFFWDSVHPTQQGWAAAISAFKSF 356

BLAST of Chy8G155320 vs. NCBI nr
Match: XP_004143379.2 (GDSL esterase/lipase At5g03610 [Cucumis sativus] >KGN48533.2 hypothetical protein Csa_003217 [Cucumis sativus])

HSP 1 Score: 657 bits (1696), Expect = 7.04e-237
Identity = 337/358 (94.13%), Postives = 341/358 (95.25%), Query Frame = 0

Query: 8   TGQGSAGGFYGNLRHHHHRHRHRSHLSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGIT 67
           +GQGSAGGFYGNLRH     RHRSH SRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGIT
Sbjct: 20  SGQGSAGGFYGNLRH-----RHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGIT 79

Query: 68  FPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTL 127
           FPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTL
Sbjct: 80  FPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTL 139

Query: 128 VPLPNMTTQIDLFEQLRHDESGL-SNRDVHLSLALVSVSGNDYSFYLATNGSTQGLKPFI 187
           VPLPNMTTQIDLFEQLR DESGL SNRDVHLSLALVSVSGNDYSFYLATNGS QGLKPFI
Sbjct: 140 VPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFI 199

Query: 188 NSVVNQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQFHNFLL 247
           NSVVNQIM+DLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFS+KQCNQTINSFVQFHNFLL
Sbjct: 200 NSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLL 259

Query: 248 KQAVDKLNKQITKQQHSSSSSSSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGLLKFKTP 307
           KQAVDKLNKQITKQQH SSSSSSS KIFILDVYDAFLSIIQGR    GSGRVGLLKFKTP
Sbjct: 260 KQAVDKLNKQITKQQHYSSSSSSS-KIFILDVYDAFLSIIQGR----GSGRVGLLKFKTP 319

Query: 308 LKPCCFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGWALAFSSFTSFL 364
           LKPCCFGVSS FECGSVDEQGN+KFVLCNDPKSAFFWDSVHPTQTGWA AFSSFTSFL
Sbjct: 320 LKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQTGWAHAFSSFTSFL 367

BLAST of Chy8G155320 vs. NCBI nr
Match: XP_008462669.1 (PREDICTED: GDSL esterase/lipase At5g03610-like, partial [Cucumis melo])

HSP 1 Score: 633 bits (1633), Expect = 1.60e-227
Identity = 322/363 (88.71%), Postives = 335/363 (92.29%), Query Frame = 0

Query: 7   KTGQGSAGGFYGNLRHHHHRHRHRSHLSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGI 66
           KTGQGSAGG+Y N RHH      RSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGI
Sbjct: 1   KTGQGSAGGYYSN-RHH------RSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGI 60

Query: 67  TFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNT 126
           TFPGKPSGRFSDGRVLTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T
Sbjct: 61  TFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDT 120

Query: 127 LVPLPNMTTQIDLFEQLRHDESGLSNRDVHLSLALVSVSGNDYSFYLATNGSTQGLKPFI 186
            VPLPNMTTQIDLFEQLRH ESGLSNRDVHLS+ALVSVSGNDYSFYLATNGS QGLKPFI
Sbjct: 121 SVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFI 180

Query: 187 NSVVNQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQFHNFLL 246
           NSVVNQ+MIDL+RI+RLGVKKIVVTGLGPLGCLPIFTAPFS+KQCNQTINSFVQFHNFLL
Sbjct: 181 NSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLL 240

Query: 247 KQAVDKLNKQITKQQHS-----SSSSSSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGLL 306
           KQAVDKLNKQIT QQHS     SSSSSSSSKIFILDVYDAFLS+IQGR    GSGRVGLL
Sbjct: 241 KQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGR----GSGRVGLL 300

Query: 307 KFKTPLKPCCFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGWALAFSSFT 364
           KFKTPLKPCCFGVSSGF CGSVDE+GN+KFVLCNDPKSAFFWDSVHPTQTGW+LAFSSFT
Sbjct: 301 KFKTPLKPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFT 352

BLAST of Chy8G155320 vs. NCBI nr
Match: KAA0025271.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 630 bits (1624), Expect = 7.33e-226
Identity = 320/362 (88.40%), Postives = 334/362 (92.27%), Query Frame = 0

Query: 8   TGQGSAGGFYGNLRHHHHRHRHRSHLSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGIT 67
           +GQGSAGG+Y N RHH      RSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGIT
Sbjct: 20  SGQGSAGGYYSN-RHH------RSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGIT 79

Query: 68  FPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTL 127
           FPGKPSGRFSDGRVLTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T 
Sbjct: 80  FPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTS 139

Query: 128 VPLPNMTTQIDLFEQLRHDESGLSNRDVHLSLALVSVSGNDYSFYLATNGSTQGLKPFIN 187
           VPLPNMTTQIDLFEQLRH ESGLSNRDVHLS+ALVSVSGNDYSFYLATNGS QGLKPFIN
Sbjct: 140 VPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFIN 199

Query: 188 SVVNQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLK 247
           SVVNQ+MIDL+RI+RLGVKKIVVTGLGPLGCLPIFTAPFS+KQCNQTINSFVQFHNFLLK
Sbjct: 200 SVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLK 259

Query: 248 QAVDKLNKQITKQQHS-----SSSSSSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGLLK 307
           QAVDKLNKQIT QQHS     SSSSSSSSKIFILDVYDAFLS+IQGR    GSGRVGLLK
Sbjct: 260 QAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGR----GSGRVGLLK 319

Query: 308 FKTPLKPCCFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGWALAFSSFTS 364
           FKTPLKPCCFGVSSGF CGSVDE+GN+KFVLCNDPKSAFFWDSVHPTQTGW+LAFSSFTS
Sbjct: 320 FKTPLKPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTS 370

BLAST of Chy8G155320 vs. NCBI nr
Match: TYK07397.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 627 bits (1617), Expect = 4.55e-225
Identity = 319/360 (88.61%), Postives = 332/360 (92.22%), Query Frame = 0

Query: 10  QGSAGGFYGNLRHHHHRHRHRSHLSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFP 69
           QGSAGG+Y N RHH      RSH  R TKLFVFGDSYVDTGNIL PFSSA+QFPYGITFP
Sbjct: 5   QGSAGGYYSN-RHH------RSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFP 64

Query: 70  GKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVP 129
           GKPSGRFSDGRVLTDFA KHLGVKSPIPFSIR+EVGEERLKESGINFAFGGTGVF+T VP
Sbjct: 65  GKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVP 124

Query: 130 LPNMTTQIDLFEQLRHDESGLSNRDVHLSLALVSVSGNDYSFYLATNGSTQGLKPFINSV 189
           LPNMTTQIDLFEQLRH ESGLSNRDVHLS+ALVSVSGNDYSFYLATNGS QGLKPFINSV
Sbjct: 125 LPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSV 184

Query: 190 VNQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLKQA 249
           VNQ+MIDL+RI+RLGVKKIVVTGLGPLGCLPIFTAPFS+KQCNQTINSFVQFHNFLLKQA
Sbjct: 185 VNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQA 244

Query: 250 VDKLNKQITKQQHS-----SSSSSSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGLLKFK 309
           VDKLNKQIT QQHS     SSSSSSSSKIFILDVYDAFLS+IQGR    GSGRVGLLKFK
Sbjct: 245 VDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGR----GSGRVGLLKFK 304

Query: 310 TPLKPCCFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGWALAFSSFTSFL 364
           TPLKPCCFGVSSGF CGSVDE+GN+KFVLCNDPKSAFFWDSVHPTQTGW+LAFSSFTS L
Sbjct: 305 TPLKPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL 353

BLAST of Chy8G155320 vs. NCBI nr
Match: XP_038883594.1 (GDSL esterase/lipase At5g03610-like [Benincasa hispida])

HSP 1 Score: 564 bits (1454), Expect = 4.38e-200
Identity = 289/363 (79.61%), Postives = 310/363 (85.40%), Query Frame = 0

Query: 1   MRLIWRKTGQGSAGGFYGNLRHHHHRHRHRSHLSRGTKLFVFGDSYVDTGNILFPFSSAE 60
           M  ++   G  SAG +YG+    HH H HR H  R TKLFVFGDSYVDTGNIL PFSSA+
Sbjct: 15  MISLFSGQGSASAGRYYGD----HHHHHHRRHRLRPTKLFVFGDSYVDTGNILLPFSSAQ 74

Query: 61  QFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGG 120
           QFPYGITFPGKPSGRFSDGRVLTDF AKHLGVKSPIPFSIRSEVG ERLKESGINFAFGG
Sbjct: 75  QFPYGITFPGKPSGRFSDGRVLTDFLAKHLGVKSPIPFSIRSEVGVERLKESGINFAFGG 134

Query: 121 TGVFNTLVPLPNMTTQIDLFEQLRHDESGLSNRDVHLSLALVSVSGNDYSFYLATNGSTQ 180
           TGVF+T VPLPNMTTQID F+ LRH ES  SN D   S+ALVSVSGNDYSFYLATNGS Q
Sbjct: 135 TGVFDTSVPLPNMTTQIDFFQHLRHVESVFSNGDAQHSVALVSVSGNDYSFYLATNGSAQ 194

Query: 181 GLKPFINSVVNQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQ 240
           GLKPFINSVVNQI+IDL+RIRRLGVKKIVVTGLGPLGCLPIFTAPFS+KQCNQTINSFV+
Sbjct: 195 GLKPFINSVVNQIVIDLKRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVR 254

Query: 241 FHNFLLKQAVDKLNKQITKQQHSSSSSSSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGL 300
           FHNFLL QAV KLNK+ TK       +SSSSKIF+LDVYDAFLSIIQ R    G     +
Sbjct: 255 FHNFLLNQAVAKLNKE-TKH------ASSSSKIFVLDVYDAFLSIIQSRSKKPGV----V 314

Query: 301 LKFKTPLKPCCFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGWALAFSSF 360
           L+FKTPLKPCCFGVSSGFECGSVDEQGN+KFVLCN+PKSAFFWDSVHPTQTGW++AFSSF
Sbjct: 315 LEFKTPLKPCCFGVSSGFECGSVDEQGNKKFVLCNNPKSAFFWDSVHPTQTGWSVAFSSF 362

Query: 361 TSF 363
            SF
Sbjct: 375 KSF 362

BLAST of Chy8G155320 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 349.7 bits (896), Expect = 2.7e-96
Identity = 180/348 (51.72%), Postives = 235/348 (67.53%), Query Frame = 0

Query: 11  GSAGGFYGNLRHHHHRHRHRSHLSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPG 70
           G   G  G+ ++HH       +  R TKLFVFGDSY DTGNI   FSS+ +FPYGITFPG
Sbjct: 22  GEINGVEGSNQNHH------LYPFRPTKLFVFGDSYADTGNIKKAFSSSWKFPYGITFPG 81

Query: 71  KPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPL 130
           KP+GRFSDGRV TDF AK +G+KSPIP+  +   G++RL + G+NFA+GGTGVFNT  PL
Sbjct: 82  KPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRL-QYGMNFAYGGTGVFNTQTPL 141

Query: 131 PNMTTQIDLFEQLRHDESGLSNRDVHLSLALVSVSGNDYSFYLATNGSTQGLKPFINSVV 190
           PNMTTQID+F+ +          ++  S+ALVSV+GNDYS ++A N        FI  VV
Sbjct: 142 PNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIALNRPASEFPAFIKQVV 201

Query: 191 NQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLKQAV 250
           +Q  ++LRRI  LGVKKI V  L PLGCLP FT   S+++CN+T N+ V  HN LL+Q V
Sbjct: 202 DQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNETQNALVNLHNNLLQQVV 261

Query: 251 DKLNKQITKQQHSSSSSSSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGLLKFKTPLKPC 310
            KLN + TKQ          S   ILD+Y+AFL++ + + S  GS R     F++PLKPC
Sbjct: 262 AKLNNE-TKQ----------STFIILDLYNAFLTVFKNKGSNPGSTR-----FESPLKPC 321

Query: 311 CFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGWALAFS 359
           C GVS  + CGSVDE+G +K+++C++PK+AFFWD +HPT+ GW   +S
Sbjct: 322 CVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYS 346

BLAST of Chy8G155320 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 310.1 bits (793), Expect = 2.4e-84
Identity = 160/348 (45.98%), Postives = 221/348 (63.51%), Query Frame = 0

Query: 12  SAGGFYGNLRHHHHRHRHRSHLSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGK 71
           S   F+      + +H +   L R  +LFVFGDSY DTGNI    S + + PYGITFP K
Sbjct: 14  SFSSFFLGAESDYPQHSNLKRL-RPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQK 73

Query: 72  PSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTL-VPL 131
           PSGRFSDGRV TDF A++LG+KSPIP++ +   G+ERL   G+N+A+GGTGVF T   PL
Sbjct: 74  PSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLL-YGMNYAYGGTGVFKTKDNPL 133

Query: 132 PNMTTQIDLFEQLRHDESGLSNRDVHLSLALVSVSGNDYSFYLATNGSTQGLKPFINSVV 191
           PNMTTQID F+++    +  S  D+  SLALVSV+GNDY+ +LA       L  F+  VV
Sbjct: 134 PNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPLTELPAFMKQVV 193

Query: 192 NQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLKQAV 251
           +QI ++  RI +LGV KIV+  + PLGCLP  T   S+++CN T N+    HN+LL +A+
Sbjct: 194 DQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLHKAI 253

Query: 252 DKLNKQITKQQHSSSSSSSSSKIFILDVYDAFLSIIQGRDSGSGSGRVGLLKFKTPLKPC 311
            +LN +           +  S   +LD Y+AFL++ + +    G  R G      PLKPC
Sbjct: 254 ARLNNE-----------TKPSTFVVLDHYNAFLTVFKNKGPEPGVSRFG-----NPLKPC 313

Query: 312 CFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGWALAFS 359
           C GV+S ++C +VDE+G +K+++C DPK+AFFWD  HP++ GW   +S
Sbjct: 314 CVGVNSSYDCSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYS 343

BLAST of Chy8G155320 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 235.7 bits (600), Expect = 5.8e-62
Identity = 131/327 (40.06%), Postives = 192/327 (58.72%), Query Frame = 0

Query: 38  KLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIP 97
           KLFVFGDSY DTGN     + A   PYGITFPGKPSGR+ DG + TDF  K LG +SP  
Sbjct: 14  KLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAESPYL 73

Query: 98  FSIRSEVGEERLKESGINFAFGGTGVFNTL--VPLPNMTTQIDLFEQLRHDESGLSNRDV 157
           +      G ++  + G+NFAFGG+ + ++    P PN+T Q++    L    +G    D+
Sbjct: 74  YRTH---GRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDL--VLAGRVYGDI 133

Query: 158 HLS-LALVSVSGNDYSFYLATNGSTQGLKPFINSVVNQIMIDLRRIRRLGVKKIVVTGLG 217
             S ++L+S +G DY +Y+  N    GLK  +  VV+ + +++  +  L  KKI VT L 
Sbjct: 134 TPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIAVTSLQ 193

Query: 218 PLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLKQAVDKLNKQ---ITKQQHSSSSSSSSS 277
           P+GCLP +T+  S+K CN++ ++ V+ HN LLK+ V KLN+Q   + K+QH         
Sbjct: 194 PIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQH--------- 253

Query: 278 KIFILDVYDAFLSIIQGRDSGSGSGRVGLLKFKTPLKPCCFGVSSGFECGSVDEQGNRKF 337
             FI+D+++AF+++++ +         G  +FK P+K CC G      CG     G + +
Sbjct: 254 -FFIIDIHNAFMTVMKNK---------GSKRFKNPMKSCCEGY-----CGR-SSDGGKLY 309

Query: 338 VLCNDPKSAFFWDSVHPTQTGWALAFS 359
            LC+DPKS FFWD+VHPTQ GW   +S
Sbjct: 314 TLCDDPKSFFFWDAVHPTQEGWRSIYS 309

BLAST of Chy8G155320 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 235.0 bits (598), Expect = 9.9e-62
Identity = 135/324 (41.67%), Postives = 185/324 (57.10%), Query Frame = 0

Query: 37  TKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPI 96
           TKLFVFGDSY DTGN  F    + +FP GITFPG P+GRFSDGRV TD+ AK++GV++PI
Sbjct: 46  TKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRTPI 105

Query: 97  PFSIRSEVGEERLK-ESGINFAFGGTGVFNTLVPL-PNMTTQIDLFEQLRHDESGLSNRD 156
            +    + G  RL  + G+NFA+GG G F T+  L P  + QID FEQL    +  S  D
Sbjct: 106 TYK-WGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLM-RNVYSPAD 165

Query: 157 VHLSLALVSVSGNDYSFYLATNGSTQGLKPFINSVVNQIMIDLRRIRRLGVKKIVVTGLG 216
           ++ S+A  S+ GNDY  Y   NGS +G       VV QI++D++RI+ LGV+K++V    
Sbjct: 166 LNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVALSP 225

Query: 217 PLGCLPIFTAPFSYKQCNQTINSFVQFHNFLLKQAVDKLNKQITKQQHSSSSSSSSSKIF 276
           P  CLP    P   K C+ T ++    HN LL++ + KLN            +++     
Sbjct: 226 PQKCLPKLVTP---KGCD-TNDTSTYLHNSLLRKGLIKLN--------DKEINNNDKSFM 285

Query: 277 ILDVYDAFLSIIQGRDSGSGSGRVGLLKFKTPLKPCCFGVSSGFECGSVDEQGNRKFVLC 336
            LD+Y+AF++I + +      G  G+  F  P K CC     G  CG     G + + LC
Sbjct: 286 TLDLYNAFVTIFKNK------GVSGVSTFPDPFKACC-ATKRGTFCGDRSLSGKKLYTLC 345

Query: 337 NDPKSAFFWDSVHPTQTGWALAFS 359
           +DPKS FFWD+VH +  GW   FS
Sbjct: 346 DDPKSFFFWDNVHISDQGWRSVFS 348

BLAST of Chy8G155320 vs. TAIR 10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 138.3 bits (347), Expect = 1.3e-32
Identity = 92/305 (30.16%), Postives = 137/305 (44.92%), Query Frame = 0

Query: 55  PFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGI 114
           P + + + PYGITFPGKPSGR+SDG   TDF              + SEV +  +  S  
Sbjct: 3   PTALSWKLPYGITFPGKPSGRYSDGLTATDF--------------LGSEVFDSPVDRS-- 62

Query: 115 NFAFGGTGVFNTLVPLPNMTTQIDLFEQLRHDESGLS-NRDVHLSLALVSVSGNDYSFYL 174
                           PN++TQ+     L       + + D+  S AL+S SG DY  ++
Sbjct: 63  ----------------PNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFI 122

Query: 175 ATNGSTQGLKPFINSVVNQIMIDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSYKQCNQ 234
             N +      F+  +V  I                                     CN+
Sbjct: 123 DQNPNMAAYPAFVEFIVEDI------------------------------------HCNE 182

Query: 235 TINSFVQFHNFLLKQAVDKLNKQITKQQHSSSSSSSSSKIFILDVYDAFLSIIQGRDSGS 294
           + +  V+ HN  LK+AV KLNK+           +   +  I+D++ AF++I++      
Sbjct: 183 SYSDLVRLHNESLKKAVAKLNKE-------DKFRTKGDRFVIVDLHKAFMTILE------ 218

Query: 295 GSGRVGLLKFKTPLKPCCFGVSSGFECGSVDEQGNRKFVLCNDPKSAFFWDSVHPTQTGW 354
              + G  +FK+PLKPCC G     +C  +D +G +K+ LCNDPKSAFFWD ++PTQ GW
Sbjct: 243 ---KKGNKRFKSPLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGW 218

Query: 355 ALAFS 359
              +S
Sbjct: 303 RSIYS 218