Chy8G155010 (gene) Cucumber (hystrix) v1

Overview
NameChy8G155010
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionThreonine dehydratase
LocationchrH08: 16386530 .. 16391796 (+)
RNA-Seq ExpressionChy8G155010
SyntenyChy8G155010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGCTCTTCTTCTCAACGCACCCACTTCACACCCAATTCCTCGTAAATCTTTCCTTCCATCCCCTCCTTCCTTCTTCAATGGCTCCTCCATTTCCATTGATGACAATCGAAACAGAGTTAGACTTACCAGGCCTCCAGTCATCCTAGCCACGCTTTCAAAGCACGCCGTTGATATCCCTCCTAATTCTGTATCCAACACTTCGGTTTCGACTCCTGGAGATGCGTTGGCGCCTACCCCTTCGCCTCCTCGCAAAAGGGTATCGGCGGATTCTCTGCAGTTCCCGCCTGGCTATCTTGGCGCTGTTCCGGACCGTTCCGGTTCTGATAATGGCGAGGATAGTGTTGACGCCATGGAGTATTTGACTAGAATATTGGGTTCGAAGGTTTATGATGTGGCTATTGAATCGCCCTTGCAGCTTGCGCCGACGTTGTCTGAACGATTTGGGGTTAATATCTGGCTTAAGAGGGAGGACTTGCAGCCTGTAAGTGAATTTTCAGTAGTTTTCATTTTGATTAATCTATTCGTTTGAAAGTTGGTATTTCTTTGGTCGTTTGTTCAAGGATTCTGTGTGAACTAGTACCCATGTTTTCAATTTAAACTTTTGTTTAACATGTTGCGTTCACTGTTGAGCATTCATCGACTTCTTTTTAGAATATCAAATTGCGGCTCGACGGTCTTTTGATTTCAACATCAGATGAAGACGGCTGATTCTGCTGATAAGGACTAATGGACGATTTTGCAAAGTAGTCATGGTTTTTAACCCCCGTTTTTTTTTTTAATTTTATTTGTAGGTGTTCTCATTCAAGCTCCGAGGAGCTTATAATATGATGGCCAAACTTCCAAAAGAACAGTTGGAAAAAGGAGTTATATGCTCTTCAGCTGGAAATCATGCTCAAGGGGTTGCATTAGCGGCTAAGCGATTAAGATGTAATGCTGTTATTGCTATGCCAGTTACTACACCTGAAATTAAGGTGCAATAATTTCTTCAGCTATGGGTTCTCTCTTTTTGTCCTGGTGTTTCATGCGGATAACAATGTTGTTCGTTTTCTAATGAAAATGAGAGCTTTTAACGTTGCAGTGGCAATCAGTTCAAAAATTGGGTGCGACTGTTGTTCTTGTGGGAGATTCATACGATGAGGCACAAGCATATGCTAAAAAGCGGAGCGTAGAGGAAGGCCGCACATTCATTCCACCTTTTGATCACCCTGATGTTATAGCAGGGCAGGGTACAGTTGGGATGGAAATTGTGCGTCAAATAAAAGGTCCAGTGCATGCAATTTTTGTACCAGTTGGGGGTGGTGGTCTAATAGCTGGTATTGCTGCTTATGTTAAAAGGGTTTCTCCTGAGGTCTGTTGGTCTCTGTTGGAGTGGATTTTTGTTTTAAGCTTTCTTATACTAACATTATTTTTTTTAGTTCAACAATAAGTGGGGGTGGGTATTCGAACAGCCTTTTGATCAAGCTTATAATGTGTTTTAACCAGTTGAGCTATGCTCATTTTTTGTTATCTGTTTACAGTACCGGCTTCATTTGACCATAAGATTTTTATTAATCTTGAATGGGATGAAGTGTCAGAATTATGGGGATTCTTGATGTTATACTATTTCTTAATATATCTATCCTTCCCTCTAAAGTAAGACTTGAAATGTTATCTATTATAGAAATATTAATTGGTCTTATTTTTCACGTCTTTCATACAGGTAAAGATCATTGGGGTAGAGCCATCTGATGCAAATGCTATGGCATTATCATTATGTCATGGCCAAAGAGTGATATTGGACAAGGCTGGAGGCTTTGCTGATGGTGTAGCAGTTAAAGAAGTCGGTGAAGAGACTTTTCGCCTTTGCAAAGATCTCATGGATGGTGTAGTTCTTGTTAGCCGTGATGCTATATGTGCCTCAATAAAGGTTTGAGTTTCTTTTCTCCCTTTCATGCACATGCGGCATTGTCTTATAACATAAAAAGATTAACTTCTCATTTGTTGAAGCCATATGACATTTCCCAGTCGATCTTTATGCTTCCATAGAAAGGATCACAATGAAGATTCAATTGAATGAACGAAAGGCTATTGCTAATTTTGATATTCTTAACACCTAATGTAGATATATTCTCAAACTATAACTGGTCGTTTTCTCCTCCTTTAAATGGTATTTGTAAAAATGATGTGATTTTTCTTGTGGAACTTGTCTTCAACATTCTTCAAGCAGTGATTTTAGCAGAGGTTTTTTATGTTTGTTATTATATCACAGGATATGTTTGAGGAGAAGAGAAGCATCTTAGAACCTGCGGGTGCTCTTTCTCTTGCTGGAGCCGAGGCCTACTGCAAATATTATGGCTTGAAGGGAGAAAATGTTGTGGTAATAACCAGTGGTGCAAATATGAACTTTGACAAACTGAGTATAGTTACTGAACTTGCCAATGTTGGTCGTGAGAAAGAGGCTGTGCTCGCAACTATATTGCCGGAGACACCTGGAAGCTTTAAAAAATTTTGTGAACTGGTTTGTTTATTAGTATTTCTAAGTTTTCTTGCTAAACATCATTCTTGGTTGATTTTCATAGTCTTTAGAATGAATGCTATAAACGGGGACTTTGTATTTTCAGGTTGGGCCTATGAACATTACAGAATTCAAGTACAGATATAGTTCTGAAAAAGAAGCTGTTGTTCTTTATAGGTAAGTGCTTATCAAGCTATTGCCAATCTGAATTAAAACTTTTTTCTAGTTTTTCAGTAGCTTTATAGGTAAGTGCTTATCAAGCCATTGGCAAGGAAAATTTAGTAATAAAAAATAGTGATTTAATGAGAATATTAACACAAAAGCAAGGTAAACGGAAAGAGGAGTTTTGCTGTAGATATTTGAAAATTTTCAGATTGGTGTGTAACTTTTTCTTCTTCTTGATGCTCAAGTTGGCTCTTGAGTGCTGATTTTGGTCGTGGTTGTGGGTTTGGATATATATTTTTTATATATATTATGTTTTTATTTCATTTTGGTTTGTTTTGTTTTTTTTCTCCCTGTTTCAAGGCAGTACTTTGGTGTCATAATAAATATTATTCGGTCTTTCAATCAGGGTCATTTACAATCGTTTGTGCCAGTCAAGTGGAAGGTCTAGCCTCTTTCATGTGTTTTTTGGTATTGATTTATACCGGAAAAATCAAGTACTTTTCTATTGATTTGGGGGCTATTTAGAGAGGAAGTAGAGAGTTTTTGGTGGGGCTTGAAGGATCTTGAGAGGAGGTGTAGTCTCCTATGTGATATAATGCTTTCTCTAGGTGATTGTCAACTAAGACTCTTTGTAACTATCATTTGGGCCTTGTACATTTGGACTAGACCCCTTTTTTGTTGAGCTAATTTGGGGCAGGGGCTCTCTCTCTTGTTGGGCTGTTTTTTTGTTTGGCCTTCTATAGTGTTTCATTTCTTAATTCTTCTCAAGGAAAATGAAGTAATTTTCTAATGATGAGATCTTGAATACTTTCAATTTTGTCATTGTTGCAGACTTTTTAATATTGTTTTATTTGATAACAGTGTTTTAAAAAGCCCTTCCAGGTCCAGATGCCACACCTTTAAAAGGAAATGCTCACAAAATAAGGCGAGCATCTTTCGTGAAGCGCTGGGCATTGGGACTTTTCATTGTTCTTAGAAATAATAATAATTAGGGTTTTTCTTTTGTTATTAACTAAAAATTCAAATTGACTAAGACTCTAAGTAGCTTAAGTGGAAATTTCTTGTATTTAAGGATTTCTTTTTTCTTCATAGTTTCACTTCAACTATGTTTTCTAGTACTTTTAGATTTAGTATGCCTCAAAAATAGCTTGTATCTTTCTTTGCTACGTGTGCTTAAGTCCCAGAAGACTCACTTTATGGCGCCTTGAGCTTTAAAAAGCACTGGTTGATAATAGGCTATCAAATTTTGCTATTTTGGGTCCTATGATGCAGCTCCTTGACTGTTTTTAAGTTGGTTTCTTCCTTATCAAGATGGTTAGCTTTGTTTTATTTATCCTTCGTGTTGGATCAGTGTTGTGGGTTGGATACTGCACACGCACAACATTTTTTATCTTAAAGAAGGAAGCATATTATAATATTTGTTTTCGTCACCATGCTTTGCAGTGTTGGTATCCATATGCCTTCTGAACTAGAGGAAATGAAGGATAGGATGGAATCGTCTCAGCTTCCAACTTATAATCTCACAAAGAATGATCTTGTAAAGGATCACTTGCGTTACTTGGTAAGTATTTTTTTTCTTTTTGGCTAATGAATTTAGGAAATGGCTACGACTATTGCTTACCTTGAGGATAGTAAAGATGCTCCATCTCAGCCTGCTTATTAATGCTCTCTGTTTATTCTTTTATACGCATATTTGTTTGTGTGTGTATAATATATGTATTTTTGAGAGAATATATGTACATATGTTTTTGTCTCTATTGTAAGGGGACATCAAACATGCATAATTGGTATGTTCATGCACTTTCAATTTGAATATTGAAAAGTTAAAAATCTTTTATCATAAGAAAACAACCATATCATCATTTATAATCTGCTGCCAATTTAAAAGAACGGTTGAAAAGAGTACCAAAGTGGAATTGACCTAATTTAGGCCTGTTTGATAACAATTGGTGTTTGCTTTTTGGTTTTTGTACATTGTGCTAGTTTTCTCCGGTGCTCTTTACAATAGCTAAGGACTTAATTCTGAAGCCTTAGCTTATTTTTATATTTTTTAGTTTTAGTTTTCAAAACCAGGCTTTTGGAACTCACGGTGGAAGTATTGTTTATTAAGTTAACCTAGAGAGATTGCCGGTTATTAAGTTAACCCTATCTTCCATGTTCTTATATTAAACAAATTATTACTCTTTGCAGATGGGAGGCAAATCAAATGTCGAAAATGAAGTTCTTTGTCGTTTTATTTTCCCAGAAAGGCCGGGTGCTCTAGAGAAGTTCTTAGATGCTTTGAGTCCTCGCTGGAACATTACTTTGTTTCATTATCAAAGCAAGGTTAAAAACTGGGCTCTAAATTTTAAATTACTCTTTCATTAGGAAGACTGTTTTCAAGAACCTTCCATCAGCTAATATATTGAACATTTTTTCATCCACAGGGTGAAACTGGTGCAAATATATTAGTTGGGCTTCAGATTGAAAAACACGACATGGGCGAATTCCATGAACGTGCTCGAAGGGTCGGATACGATTACGTTGTGGTAACTGATGATGGTATCTTTCAGCTTCTGATGCATCATAGACCAACCCACAAGGCCATAGATCTCTTAAGTTGA

mRNA sequence

ATGGACGCTCTTCTTCTCAACGCACCCACTTCACACCCAATTCCTCGTAAATCTTTCCTTCCATCCCCTCCTTCCTTCTTCAATGGCTCCTCCATTTCCATTGATGACAATCGAAACAGAGTTAGACTTACCAGGCCTCCAGTCATCCTAGCCACGCTTTCAAAGCACGCCGTTGATATCCCTCCTAATTCTGTATCCAACACTTCGGTTTCGACTCCTGGAGATGCGTTGGCGCCTACCCCTTCGCCTCCTCGCAAAAGGGTATCGGCGGATTCTCTGCAGTTCCCGCCTGGCTATCTTGGCGCTGTTCCGGACCGTTCCGGTTCTGATAATGGCGAGGATAGTGTTGACGCCATGGAGTATTTGACTAGAATATTGGGTTCGAAGGTTTATGATGTGGCTATTGAATCGCCCTTGCAGCTTGCGCCGACGTTGTCTGAACGATTTGGGGTTAATATCTGGCTTAAGAGGGAGGACTTGCAGCCTGTGTTCTCATTCAAGCTCCGAGGAGCTTATAATATGATGGCCAAACTTCCAAAAGAACAGTTGGAAAAAGGAGTTATATGCTCTTCAGCTGGAAATCATGCTCAAGGGGTTGCATTAGCGGCTAAGCGATTAAGATGTAATGCTGTTATTGCTATGCCAGTTACTACACCTGAAATTAAGTGGCAATCAGTTCAAAAATTGGGTGCGACTGTTGTTCTTGTGGGAGATTCATACGATGAGGCACAAGCATATGCTAAAAAGCGGAGCGTAGAGGAAGGCCGCACATTCATTCCACCTTTTGATCACCCTGATGTTATAGCAGGGCAGGGTACAGTTGGGATGGAAATTGTGCGTCAAATAAAAGGTCCAGTGCATGCAATTTTTGTACCAGTTGGGGGTGGTGGTCTAATAGCTGGTATTGCTGCTTATGTTAAAAGGGTTTCTCCTGAGGTAAAGATCATTGGGGTAGAGCCATCTGATGCAAATGCTATGGCATTATCATTATGTCATGGCCAAAGAGTGATATTGGACAAGGCTGGAGGCTTTGCTGATGGTGTAGCAGTTAAAGAAGTCGGTGAAGAGACTTTTCGCCTTTGCAAAGATCTCATGGATGGTGTAGTTCTTGTTAGCCGTGATGCTATATGTGCCTCAATAAAGGATATGTTTGAGGAGAAGAGAAGCATCTTAGAACCTGCGGGTGCTCTTTCTCTTGCTGGAGCCGAGGCCTACTGCAAATATTATGGCTTGAAGGGAGAAAATGTTGTGGTAATAACCAGTGGTGCAAATATGAACTTTGACAAACTGAGTATAGTTACTGAACTTGCCAATGTTGGTCGTGAGAAAGAGGCTGTGCTCGCAACTATATTGCCGGAGACACCTGGAAGCTTTAAAAAATTTTGTGAACTGGTTGGGCCTATGAACATTACAGAATTCAAGTACAGATATAGTTCTGAAAAAGAAGCTGTTGTTCTTTATAGTGTTGGTATCCATATGCCTTCTGAACTAGAGGAAATGAAGGATAGGATGGAATCGTCTCAGCTTCCAACTTATAATCTCACAAAGAATGATCTTGTAAAGGATCACTTGCGTTACTTGATGGGAGGCAAATCAAATGTCGAAAATGAAGTTCTTTGTCGTTTTATTTTCCCAGAAAGGCCGGGTGCTCTAGAGAAGTTCTTAGATGCTTTGAGTCCTCGCTGGAACATTACTTTGTTTCATTATCAAAGCAAGGGTGAAACTGGTGCAAATATATTAGTTGGGCTTCAGATTGAAAAACACGACATGGGCGAATTCCATGAACGTGCTCGAAGGGTCGGATACGATTACGTTGTGGTAACTGATGATGGTATCTTTCAGCTTCTGATGCATCATAGACCAACCCACAAGGCCATAGATCTCTTAAGTTGA

Coding sequence (CDS)

ATGGACGCTCTTCTTCTCAACGCACCCACTTCACACCCAATTCCTCGTAAATCTTTCCTTCCATCCCCTCCTTCCTTCTTCAATGGCTCCTCCATTTCCATTGATGACAATCGAAACAGAGTTAGACTTACCAGGCCTCCAGTCATCCTAGCCACGCTTTCAAAGCACGCCGTTGATATCCCTCCTAATTCTGTATCCAACACTTCGGTTTCGACTCCTGGAGATGCGTTGGCGCCTACCCCTTCGCCTCCTCGCAAAAGGGTATCGGCGGATTCTCTGCAGTTCCCGCCTGGCTATCTTGGCGCTGTTCCGGACCGTTCCGGTTCTGATAATGGCGAGGATAGTGTTGACGCCATGGAGTATTTGACTAGAATATTGGGTTCGAAGGTTTATGATGTGGCTATTGAATCGCCCTTGCAGCTTGCGCCGACGTTGTCTGAACGATTTGGGGTTAATATCTGGCTTAAGAGGGAGGACTTGCAGCCTGTGTTCTCATTCAAGCTCCGAGGAGCTTATAATATGATGGCCAAACTTCCAAAAGAACAGTTGGAAAAAGGAGTTATATGCTCTTCAGCTGGAAATCATGCTCAAGGGGTTGCATTAGCGGCTAAGCGATTAAGATGTAATGCTGTTATTGCTATGCCAGTTACTACACCTGAAATTAAGTGGCAATCAGTTCAAAAATTGGGTGCGACTGTTGTTCTTGTGGGAGATTCATACGATGAGGCACAAGCATATGCTAAAAAGCGGAGCGTAGAGGAAGGCCGCACATTCATTCCACCTTTTGATCACCCTGATGTTATAGCAGGGCAGGGTACAGTTGGGATGGAAATTGTGCGTCAAATAAAAGGTCCAGTGCATGCAATTTTTGTACCAGTTGGGGGTGGTGGTCTAATAGCTGGTATTGCTGCTTATGTTAAAAGGGTTTCTCCTGAGGTAAAGATCATTGGGGTAGAGCCATCTGATGCAAATGCTATGGCATTATCATTATGTCATGGCCAAAGAGTGATATTGGACAAGGCTGGAGGCTTTGCTGATGGTGTAGCAGTTAAAGAAGTCGGTGAAGAGACTTTTCGCCTTTGCAAAGATCTCATGGATGGTGTAGTTCTTGTTAGCCGTGATGCTATATGTGCCTCAATAAAGGATATGTTTGAGGAGAAGAGAAGCATCTTAGAACCTGCGGGTGCTCTTTCTCTTGCTGGAGCCGAGGCCTACTGCAAATATTATGGCTTGAAGGGAGAAAATGTTGTGGTAATAACCAGTGGTGCAAATATGAACTTTGACAAACTGAGTATAGTTACTGAACTTGCCAATGTTGGTCGTGAGAAAGAGGCTGTGCTCGCAACTATATTGCCGGAGACACCTGGAAGCTTTAAAAAATTTTGTGAACTGGTTGGGCCTATGAACATTACAGAATTCAAGTACAGATATAGTTCTGAAAAAGAAGCTGTTGTTCTTTATAGTGTTGGTATCCATATGCCTTCTGAACTAGAGGAAATGAAGGATAGGATGGAATCGTCTCAGCTTCCAACTTATAATCTCACAAAGAATGATCTTGTAAAGGATCACTTGCGTTACTTGATGGGAGGCAAATCAAATGTCGAAAATGAAGTTCTTTGTCGTTTTATTTTCCCAGAAAGGCCGGGTGCTCTAGAGAAGTTCTTAGATGCTTTGAGTCCTCGCTGGAACATTACTTTGTTTCATTATCAAAGCAAGGGTGAAACTGGTGCAAATATATTAGTTGGGCTTCAGATTGAAAAACACGACATGGGCGAATTCCATGAACGTGCTCGAAGGGTCGGATACGATTACGTTGTGGTAACTGATGATGGTATCTTTCAGCTTCTGATGCATCATAGACCAACCCACAAGGCCATAGATCTCTTAAGTTGA

Protein sequence

MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPVILATLSKHAVDIPPNSVSNTSVSTPGDALAPTPSPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAMEYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSEKEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLCRFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKHDMGEFHERARRVGYDYVVVTDDGIFQLLMHHRPTHKAIDLLS*
Homology
BLAST of Chy8G155010 vs. ExPASy Swiss-Prot
Match: Q9ZSS6 (Threonine dehydratase biosynthetic, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OMR1 PE=1 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 3.3e-243
Identity = 425/583 (72.90%), Postives = 488/583 (83.70%), Query Frame = 0

Query: 38  RNRVRLTRPPVILAT--LSKHAVDIPPNSVSNTSVSTPGDALAPTPSPPRKRVSADSLQF 97
           R+ +     PV+  T   S+  + +   S   TS++ P     P    PR +VS +SLQ+
Sbjct: 14  RSHIHRPSKPVVGFTHFSSRSRIAVAVLSRDETSMTPP----PPKLPLPRLKVSPNSLQY 73

Query: 98  PPGYLGAVPDRSGSDNGEDSVDAMEYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWL 157
           P GYLGAVP+R+         +AMEYLT IL +KVYD+AIESPLQLA  LS+R GV ++L
Sbjct: 74  PAGYLGAVPERTNEAENGSIAEAMEYLTNILSTKVYDIAIESPLQLAKKLSKRLGVRMYL 133

Query: 158 KREDLQPVFSFKLRGAYNMMAKLPKEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMP 217
           KREDLQPVFSFKLRGAYNMM KLP +QL KGVICSSAGNHAQGVAL+A +L C AVI MP
Sbjct: 134 KREDLQPVFSFKLRGAYNMMVKLPADQLAKGVICSSAGNHAQGVALSASKLGCTAVIVMP 193

Query: 218 VTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVG 277
           VTTPEIKWQ+V+ LGATVVL GDSYD+AQA+AK R+ EEG TFIPPFDHPDVIAGQGTVG
Sbjct: 194 VTTPEIKWQAVENLGATVVLFGDSYDQAQAHAKIRAEEEGLTFIPPFDHPDVIAGQGTVG 253

Query: 278 MEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQR 337
           MEI RQ KGP+HAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEP+DANAMALSL HG+R
Sbjct: 254 MEITRQAKGPLHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPADANAMALSLHHGER 313

Query: 338 VILDKAGGFADGVAVKEVGEETFRLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAG 397
           VILD+ GGFADGVAVKEVGEETFR+ ++LMDGVVLV+RDAICASIKDMFEEKR+ILEPAG
Sbjct: 314 VILDQVGGFADGVAVKEVGEETFRISRNLMDGVVLVTRDAICASIKDMFEEKRNILEPAG 373

Query: 398 ALSLAGAEAYCKYYGLKGENVVVITSGANMNFDKLSIVTELANVGREKEAVLATILPETP 457
           AL+LAGAEAYCKYYGLK  NVV ITSGANMNFDKL IVTELANVGR++EAVLAT++PE P
Sbjct: 374 ALALAGAEAYCKYYGLKDVNVVAITSGANMNFDKLRIVTELANVGRQQEAVLATLMPEKP 433

Query: 458 GSFKKFCELVGPMNITEFKYRYSSEKEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLT 517
           GSFK+FCELVGPMNI+EFKYR SSEKEAVVLYSVG+H   EL+ ++ RMESSQL T NLT
Sbjct: 434 GSFKQFCELVGPMNISEFKYRCSSEKEAVVLYSVGVHTAGELKALQKRMESSQLKTVNLT 493

Query: 518 KNDLVKDHLRYLMGGKSNVENEVLCRFIFPERPGALEKFLDALSPRWNITLFHYQSKGET 577
            +DLVKDHLRYLMGG+S V +EVLCRF FPERPGAL  FLD+ SPRWNITLFHY+ +GET
Sbjct: 494 TSDLVKDHLRYLMGGRSTVGDEVLCRFTFPERPGALMNFLDSFSPRWNITLFHYRGQGET 553

Query: 578 GANILVGLQIEKHDMGEFHERARRVGYDYVVVTDDGIFQLLMH 619
           GAN+LVG+Q+ + +M EF  RA+ +GYDY +V+DD  F+LLMH
Sbjct: 554 GANVLVGIQVPEQEMEEFKNRAKALGYDYFLVSDDDYFKLLMH 592

BLAST of Chy8G155010 vs. ExPASy Swiss-Prot
Match: A0FKE6 (Threonine dehydratase 1 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4081 GN=TD1 PE=1 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 9.6e-235
Identity = 417/596 (69.97%), Postives = 487/596 (81.71%), Query Frame = 0

Query: 24  PSFFNGSSISIDDNRNRVRLTRPPVILATLSKHAVDIPPNSVSNTSVSTPGDALAPTPSP 83
           P F   SS+ +  +  +V  TR     A +   A +I  +  + T       A AP    
Sbjct: 18  PEFTAMSSVIVPISTVKVSGTRKSKKKALICAKATEILSSPATVTEPLKAEPAEAPV--- 77

Query: 84  PRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAMEYLTRILGSKVYDVAIESPLQLAP 143
           P  RVS  SLQ  PGYL  +P+      G   V   EYLT IL SKVYDVA E+PLQ AP
Sbjct: 78  PLLRVSPSSLQCEPGYL--LPNSPVLGTG--GVTGYEYLTNILSSKVYDVAYETPLQKAP 137

Query: 144 TLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQLEKGVICSSAGNHAQGVALAA 203
            LSER GVN+WLKREDLQPVFSFK+RGAYNMMAKLPKEQLEKGVICSSAGNHAQGVAL+A
Sbjct: 138 KLSERLGVNVWLKREDLQPVFSFKIRGAYNMMAKLPKEQLEKGVICSSAGNHAQGVALSA 197

Query: 204 KRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSVEEGRTFIPPFD 263
           +RL C+AVI MPVTTP+IKW+SV++LGATVVLVGDSYDEAQAYAKKR+  EGRTFIPPFD
Sbjct: 198 QRLGCDAVIVMPVTTPDIKWKSVKRLGATVVLVGDSYDEAQAYAKKRAESEGRTFIPPFD 257

Query: 264 HPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDA 323
           HPDVI GQGTVGMEI RQ+K  +HAIFVPVGGGGLIAGIAAY+KRV+P++KIIGVEP DA
Sbjct: 258 HPDVIVGQGTVGMEINRQLKDNIHAIFVPVGGGGLIAGIAAYLKRVAPDIKIIGVEPLDA 317

Query: 324 NAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKDLMDGVVLVSRDAICASIKDM 383
           NA+ALSL HGQRV+LD+ GGFADGVAVK VGEET+RLC++L+DGVVLV RDAICASIKDM
Sbjct: 318 NALALSLHHGQRVMLDQVGGFADGVAVKVVGEETYRLCEELIDGVVLVGRDAICASIKDM 377

Query: 384 FEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGANMNFDKLSIVTELANVGREK 443
           FEEKRSILEPAGAL+LAGAEAYCKYYGLKGENVV ITSGANMNFD+L +VTELA+VGR++
Sbjct: 378 FEEKRSILEPAGALALAGAEAYCKYYGLKGENVVAITSGANMNFDRLRLVTELADVGRQR 437

Query: 444 EAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSEKE-AVVLYSVGIHMPSELEEMKD 503
           EAVLAT +PE PGSFKKF E+VGPMNITEFKYRY+S+KE A+VLYSVG+H   ELE M +
Sbjct: 438 EAVLATFMPEDPGSFKKFAEMVGPMNITEFKYRYNSDKERALVLYSVGLHTILELEGMVE 497

Query: 504 RMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLCRFIFPERPGALEKFLDALSPRW 563
           RMES+ L T NLT NDLVKDHLR+LMGG++NV NE+LCRF FPE+PGAL KFLDA SPRW
Sbjct: 498 RMESADLQTINLTDNDLVKDHLRHLMGGRTNVHNELLCRFTFPEKPGALMKFLDAFSPRW 557

Query: 564 NITLFHYQSKGETGANILVGLQIEKHDMGEFHERARRVGYDYVVVTDDGIFQLLMH 619
           NI+LFHY+++G+TGAN+LVG+Q+   ++ EF  RA  +GY+Y + + +  +QL+MH
Sbjct: 558 NISLFHYRAQGDTGANVLVGIQVPPDEVVEFEGRADSLGYEYAMESLNEAYQLIMH 606

BLAST of Chy8G155010 vs. ExPASy Swiss-Prot
Match: Q9AXU4 (Threonine dehydratase OS=Nicotiana attenuata OX=49451 GN=TD PE=1 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 4.1e-193
Identity = 333/532 (62.59%), Postives = 420/532 (78.95%), Query Frame = 0

Query: 89  SADSLQFPPGYLGAVPDR--SGSDNGEDSVDAMEYLTRILGSKVYDVAIESPLQLAPTLS 148
           SADS+  P         +    + +G D+ +  +YL  IL S+VYDVAI+SPLQ A  LS
Sbjct: 70  SADSMPVPASLQDVEAGKLIENNPSGGDTEELFQYLVEILASRVYDVAIDSPLQNAAKLS 129

Query: 149 ERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQLEKGVICSSAGNHAQGVALAAKRL 208
           ++ GVN W+KRED+Q VFSFKLRGAYNMM KL KEQLE+GVI +SAGNHAQGVAL A+RL
Sbjct: 130 KKLGVNFWIKREDMQSVFSFKLRGAYNMMTKLSKEQLERGVITASAGNHAQGVALGAQRL 189

Query: 209 RCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSVEEGRTFIPPFDHPD 268
           +C A I MPVTTPEIK ++V+ L   VVL GD++D+AQ +A K + +EG TFIPPFDHPD
Sbjct: 190 KCTATIVMPVTTPEIKIEAVKNLDGKVVLHGDTFDKAQEHALKLAEDEGLTFIPPFDHPD 249

Query: 269 VIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDANAM 328
           VI GQGT+G EI RQ+K  +HA+FVPVGGGGLIAG+AAY KRV+P  KIIGVEP  A++M
Sbjct: 250 VIIGQGTIGTEINRQLK-DIHAVFVPVGGGGLIAGVAAYFKRVAPHTKIIGVEPFGASSM 309

Query: 329 ALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKDLMDGVVLVSRDAICASIKDMFEE 388
             SL HG+RV L++   FADGVAV  VGEETFRLCKDL+DG+VLVS DAI A++KD+++E
Sbjct: 310 TQSLYHGERVKLEQVDNFADGVAVALVGEETFRLCKDLIDGMVLVSNDAISAAVKDVYDE 369

Query: 389 KRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGANMNFDKLSIVTELANVGREKEAV 448
            R+ILE +GAL++AGAEAYCKYY +KGENVV I SGANM+F KL +V +LA++G ++EA+
Sbjct: 370 GRNILETSGALAIAGAEAYCKYYNIKGENVVAIASGANMDFSKLKLVVDLADIGGQREAL 429

Query: 449 LATILPETPGSFKKFCELVGPMNITEFKYRYSS-EKEAVVLYSVGIHMPSELEEMKDRME 508
           LAT +PE PGSFKKFCELVGPMNITEFKYRY+S  K+A+VLYSVG++  S+LE M +RM+
Sbjct: 430 LATFMPEEPGSFKKFCELVGPMNITEFKYRYNSGRKQALVLYSVGVNTKSDLESMLERMK 489

Query: 509 SSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLCRFIFPERPGALEKFLDALSPRWNIT 568
           SSQL T NLT N+LVK+HLR+LMGG+S   NE+ C+FIFPE+PGAL KFLDA SPRWNI+
Sbjct: 490 SSQLNTVNLTNNNLVKEHLRHLMGGRSEPSNEIFCQFIFPEKPGALRKFLDAFSPRWNIS 549

Query: 569 LFHYQSKGETGANILVGLQIEKHDMGEFHERARRVGYDYVVVTDDGIFQLLM 618
           LFHY+ +GE  A++LVG Q+ K ++ EF  +A  +GY Y + + +   QL+M
Sbjct: 550 LFHYREQGELDASVLVGFQVPKGEIEEFRVQANNLGYSYEIESLNEASQLIM 600

BLAST of Chy8G155010 vs. ExPASy Swiss-Prot
Match: P25306 (Threonine dehydratase 2 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4081 GN=TD2 PE=1 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 8.9e-156
Identity = 277/507 (54.64%), Postives = 371/507 (73.18%), Query Frame = 0

Query: 112 GEDSVDAMEYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGA 171
           G DS +  +YL  IL S VYDVAIESPL+LA  LS+R GVN ++KRED Q VFSFKLRGA
Sbjct: 88  GGDSDELFQYLVDILASPVYDVAIESPLELAEKLSDRLGVNFYIKREDKQRVFSFKLRGA 147

Query: 172 YNMMAKLPKEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGA 231
           YNMM+ L +E+L+KGVI +SAGNHAQGVALA +RL C A I MP TTP+IK  +V+ LG 
Sbjct: 148 YNMMSNLSREELDKGVITASAGNHAQGVALAGQRLNCVAKIVMPTTTPQIKIDAVRALGG 207

Query: 232 TVVLVGDSYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFV 291
            VVL G ++DEAQ +A + S ++G  +IPPFD P VI GQGT+G EI RQ+K  +HA+F+
Sbjct: 208 DVVLYGKTFDEAQTHALELSEKDGLKYIPPFDDPGVIKGQGTIGTEINRQLK-DIHAVFI 267

Query: 292 PVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVK 351
           PVGGGGLIAG+A + K+++P  KIIGVEP  A +M LSL  G RV L     FADGVAV 
Sbjct: 268 PVGGGGLIAGVATFFKQIAPNTKIIGVEPYGAASMTLSLHEGHRVKLSNVDTFADGVAVA 327

Query: 352 EVGEETFRLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGL 411
            VGE TF  C++L+DG+VLV+ D I A+IKD+++E R+ILE +GA+++AGA AYC++Y +
Sbjct: 328 LVGEYTFAKCQELIDGMVLVANDGISAAIKDVYDEGRNILETSGAVAIAGAAAYCEFYKI 387

Query: 412 KGENVVVITSGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNIT 471
           K EN+V I SGANM+F KL  VTELA +G  KEA+LAT + E  GSFK F  LVG +N T
Sbjct: 388 KNENIVAIASGANMDFSKLHKVTELAGLGSGKEALLATFMVEQQGSFKTFVGLVGSLNFT 447

Query: 472 EFKYRYSSE-KEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGG 531
           E  YR++SE K A++LY V +   S+LE+M + M+SS + T NL+ N+LV DHL++L+GG
Sbjct: 448 ELTYRFTSERKNALILYRVNVDKESDLEKMIEDMKSSNMTTLNLSHNELVVDHLKHLVGG 507

Query: 532 KSNVENEVLCRFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKHDM 591
            +N+ +E+   FI PE+   L+ FLDA SPRWNITL  Y+++G+  A++L+G Q+ + +M
Sbjct: 508 SANISDEIFGEFIVPEKAETLKTFLDAFSPRWNITLCRYRNQGDINASLLMGFQVPQAEM 567

Query: 592 GEFHERARRVGYDYVVVTDDGIFQLLM 618
            EF  +A ++GY Y +   +  F L++
Sbjct: 568 DEFKNQADKLGYPYELDNYNEAFNLVV 593

BLAST of Chy8G155010 vs. ExPASy Swiss-Prot
Match: P53607 (L-threonine dehydratase biosynthetic IlvA OS=Burkholderia multivorans (strain ATCC 17616 / 249) OX=395019 GN=ilvA PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 2.9e-146
Identity = 263/502 (52.39%), Postives = 355/502 (70.72%), Query Frame = 0

Query: 120 EYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLP 179
           +YL +IL ++VYDVA E+ L+ A  LS R    ++LKRED QPVFSFKLRGAYN MA +P
Sbjct: 5   DYLKKILTARVYDVAFETELEPARNLSARLRNPVYLKREDNQPVFSFKLRGAYNKMAHIP 64

Query: 180 KEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQK---LGATVVLV 239
            + L +GVI +SAGNHAQGVA +A R+   AVI +PVTTP++K  +V+     G  V+  
Sbjct: 65  ADALARGVITASAGNHAQGVAFSAARMGVKAVIVVPVTTPQVKVDAVRAHGGPGVEVIQA 124

Query: 240 GDSYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGG 299
           G+SY +A A+A K   E G TF+ PFD P VIAGQGT+ MEI+RQ +GP+HAIFVP+GGG
Sbjct: 125 GESYSDAYAHALKVQEERGLTFVHPFDDPYVIAGQGTIAMEILRQHQGPIHAIFVPIGGG 184

Query: 300 GLIAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEE 359
           GL AG+AAYVK V PE+K+IGV+  D+ AMA SL  G+RV L + G FADG AVK VGEE
Sbjct: 185 GLAAGVAAYVKAVRPEIKVIGVQAEDSCAMAQSLQAGKRVELAEVGLFADGTAVKLVGEE 244

Query: 360 TFRLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENV 419
           TFRLCK+ +DGVV V  DA+CA+IKD+F++ RS+LEP+GAL++AGA+ Y +  G++ + +
Sbjct: 245 TFRLCKEYLDGVVTVDTDALCAAIKDVFQDTRSVLEPSGALAVAGAKLYAEREGIENQTL 304

Query: 420 VVITSGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYR 479
           V +TSGANMNFD++  V E A VG  +EAV A  +PE  GSFK+FC LVG  N+TEF YR
Sbjct: 305 VAVTSGANMNFDRMRFVAERAEVGEAREAVFAVTIPEERGSFKRFCSLVGDRNVTEFNYR 364

Query: 480 YSSEKEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVE- 539
            +  + A +   V I    E  ++    ES    T +LT ++L K+H+RY++GG+S +  
Sbjct: 365 IADAQSAHIFVGVQIRRRGESADIAANFESHGFKTADLTHDELSKEHIRYMVGGRSPLAL 424

Query: 540 NEVLCRFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKHDMGEFHE 599
           +E L RF FPERPGAL KFL +++P WNI+LFHY+++G   ++ILVGLQ+ + D  EF  
Sbjct: 425 DERLFRFEFPERPGALMKFLSSMAPDWNISLFHYRNQGADYSSILVGLQVPQADHAEFER 484

Query: 600 RARRVGYDYVVVTDDGIFQLLM 618
               +GY YV  + +  ++L +
Sbjct: 485 FLAALGYPYVEESANPAYRLFL 506

BLAST of Chy8G155010 vs. ExPASy TrEMBL
Match: A0A0A0KII4 (Threonine dehydratase OS=Cucumis sativus OX=3659 GN=Csa_6G449240 PE=3 SV=1)

HSP 1 Score: 1244.2 bits (3218), Expect = 0.0e+00
Identity = 623/630 (98.89%), Postives = 626/630 (99.37%), Query Frame = 0

Query: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPVILATLSKHAVDI 60
           MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPP+ILATLSKHA+DI
Sbjct: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDI 60

Query: 61  PPNSVSNTSVSTPGDALAPTPSPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120
           PPNSVSNTSV TP DALAPTP PPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME
Sbjct: 61  PPNSVSNTSVPTPADALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120

Query: 121 YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK 180
           YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK
Sbjct: 121 YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK 180

Query: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240
           EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY
Sbjct: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240

Query: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300
           DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA
Sbjct: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300

Query: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360
           GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL
Sbjct: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360

Query: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420
           CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT
Sbjct: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420

Query: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480
           SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE
Sbjct: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480

Query: 481 KEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC 540
           KEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC
Sbjct: 481 KEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC 540

Query: 541 RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKHDMGEFHERARRV 600
           RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEK DMGEFHERARRV
Sbjct: 541 RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKPDMGEFHERARRV 600

Query: 601 GYDYVVVTDDGIFQLLMHHRPTHKAIDLLS 631
           GYDYVVVTDDGIFQLLMHHRP+HKAIDLLS
Sbjct: 601 GYDYVVVTDDGIFQLLMHHRPSHKAIDLLS 630

BLAST of Chy8G155010 vs. ExPASy TrEMBL
Match: A0A5A7SGN7 (Threonine dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00800 PE=3 SV=1)

HSP 1 Score: 1232.6 bits (3188), Expect = 0.0e+00
Identity = 617/630 (97.94%), Postives = 623/630 (98.89%), Query Frame = 0

Query: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPVILATLSKHAVDI 60
           M+ALLLNAPTSHPIPRKSFLPSPPSFFNG+SISIDDNRNRVRLTRPP I ATLSKHAVDI
Sbjct: 1   MEALLLNAPTSHPIPRKSFLPSPPSFFNGTSISIDDNRNRVRLTRPPFIQATLSKHAVDI 60

Query: 61  PPNSVSNTSVSTPGDALAPTPSPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120
           PPNSVSNTS+ TPGDALAPTP PPR+RVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME
Sbjct: 61  PPNSVSNTSIPTPGDALAPTPLPPRRRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120

Query: 121 YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK 180
           YLTRILGSKVYDVAIESPLQLAPTLSER G NIWLKREDLQPVFSFKLRGAYNMMAKLP+
Sbjct: 121 YLTRILGSKVYDVAIESPLQLAPTLSERLGNNIWLKREDLQPVFSFKLRGAYNMMAKLPQ 180

Query: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240
           EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY
Sbjct: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240

Query: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300
           DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA
Sbjct: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300

Query: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360
           GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL
Sbjct: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360

Query: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420
           CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT
Sbjct: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420

Query: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480
           SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE
Sbjct: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480

Query: 481 KEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC 540
           KEAVVLYSVGIHMPSELEEMK+RMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC
Sbjct: 481 KEAVVLYSVGIHMPSELEEMKNRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC 540

Query: 541 RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKHDMGEFHERARRV 600
           RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEK DMGEFHERARRV
Sbjct: 541 RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKPDMGEFHERARRV 600

Query: 601 GYDYVVVTDDGIFQLLMHHRPTHKAIDLLS 631
           GYDYVVVTDDGIFQLLMHHRPTHKAIDLLS
Sbjct: 601 GYDYVVVTDDGIFQLLMHHRPTHKAIDLLS 630

BLAST of Chy8G155010 vs. ExPASy TrEMBL
Match: A0A1S3CHM7 (Threonine dehydratase OS=Cucumis melo OX=3656 GN=LOC103500854 PE=3 SV=1)

HSP 1 Score: 1232.6 bits (3188), Expect = 0.0e+00
Identity = 617/630 (97.94%), Postives = 623/630 (98.89%), Query Frame = 0

Query: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPVILATLSKHAVDI 60
           M+ALLLNAPTSHPIPRKSFLPSPPSFFNG+SISIDDNRNRVRLTRPP I ATLSKHAVDI
Sbjct: 1   MEALLLNAPTSHPIPRKSFLPSPPSFFNGTSISIDDNRNRVRLTRPPFIQATLSKHAVDI 60

Query: 61  PPNSVSNTSVSTPGDALAPTPSPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120
           PPNSVSNTS+ TPGDALAPTP PPR+RVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME
Sbjct: 61  PPNSVSNTSIPTPGDALAPTPLPPRRRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120

Query: 121 YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK 180
           YLTRILGSKVYDVAIESPLQLAPTLSER G NIWLKREDLQPVFSFKLRGAYNMMAKLP+
Sbjct: 121 YLTRILGSKVYDVAIESPLQLAPTLSERLGNNIWLKREDLQPVFSFKLRGAYNMMAKLPQ 180

Query: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240
           EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY
Sbjct: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240

Query: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300
           DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA
Sbjct: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300

Query: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360
           GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL
Sbjct: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360

Query: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420
           CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT
Sbjct: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420

Query: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480
           SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE
Sbjct: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480

Query: 481 KEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC 540
           KEAVVLYSVGIHMPSELEEMK+RMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC
Sbjct: 481 KEAVVLYSVGIHMPSELEEMKNRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC 540

Query: 541 RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKHDMGEFHERARRV 600
           RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEK DMGEFHERARRV
Sbjct: 541 RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKPDMGEFHERARRV 600

Query: 601 GYDYVVVTDDGIFQLLMHHRPTHKAIDLLS 631
           GYDYVVVTDDGIFQLLMHHRPTHKAIDLLS
Sbjct: 601 GYDYVVVTDDGIFQLLMHHRPTHKAIDLLS 630

BLAST of Chy8G155010 vs. ExPASy TrEMBL
Match: A0A6J1IPE0 (Threonine dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111477608 PE=3 SV=1)

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 547/624 (87.66%), Postives = 579/624 (92.79%), Query Frame = 0

Query: 4   LLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPVILATLSKHAVDIPPN 63
           +LLNAPTSHP+ RK  L SP  F NG+SI IDDNRNR+R+TR P I ATLSKH V+  PN
Sbjct: 3   VLLNAPTSHPLSRKWSLLSPAFFLNGTSIPIDDNRNRIRVTRVPFIQATLSKHTVENLPN 62

Query: 64  SVSNTSVSTPGDALAPTPSPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAMEYLT 123
           SVSNT+V    DALA  P PPRKRV ADSLQFPPGYLGAVPDR GSD+ EDS+DAMEYLT
Sbjct: 63  SVSNTAVVALEDALAAVPLPPRKRVLADSLQFPPGYLGAVPDRLGSDDEEDSLDAMEYLT 122

Query: 124 RILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL 183
           RILGSKVYDVAIESPLQLAP LSER GVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL
Sbjct: 123 RILGSKVYDVAIESPLQLAPMLSERLGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL 182

Query: 184 EKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA 243
           E+GVICSSAGNHAQGVALAA+RLRC+AVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA
Sbjct: 183 ERGVICSSAGNHAQGVALAARRLRCDAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA 242

Query: 244 QAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIA 303
           QAYAKKRS++EGRTFIPPFDHPDVIAGQGTVGMEIVRQ+K  +HAIFVPVGGGGLIAGIA
Sbjct: 243 QAYAKKRSIDEGRTFIPPFDHPDVIAGQGTVGMEIVRQMKSKLHAIFVPVGGGGLIAGIA 302

Query: 304 AYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKD 363
           AYVKRVSPEVKIIGVEPSDANAMALSLCHGQR+IL+KAGGFADGVAVKEVGEETFRLCK 
Sbjct: 303 AYVKRVSPEVKIIGVEPSDANAMALSLCHGQRIILEKAGGFADGVAVKEVGEETFRLCKG 362

Query: 364 LMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA 423
           LMDGVVLV RDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA
Sbjct: 363 LMDGVVLVGRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA 422

Query: 424 NMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSEKEA 483
           NMNFDKL IVTELANVGRE+EAVL T LPE PGSFK+FCEL+G MNITEFKYRY+SEKEA
Sbjct: 423 NMNFDKLRIVTELANVGREQEAVLVTKLPEKPGSFKRFCELIGAMNITEFKYRYNSEKEA 482

Query: 484 VVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLCRFI 543
           VVLYSVG+HMPSELEEM +RM SSQL TYNLT NDLVKDHLRYLMGG+SNVENEVLCRFI
Sbjct: 483 VVLYSVGMHMPSELEEMMNRMNSSQLETYNLTNNDLVKDHLRYLMGGRSNVENEVLCRFI 542

Query: 544 FPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKHDMGEFHERARRVGYD 603
           FPERPGALEKFLDA SPRWNI+LFHY  +GETGANI VGLQ+EK ++GEFHERARRVGYD
Sbjct: 543 FPERPGALEKFLDAFSPRWNISLFHYHGQGETGANIFVGLQMEKPELGEFHERARRVGYD 602

Query: 604 YVVVTDDGIFQLLMHHRPTHKAID 628
           YVVVTDDGIFQLLMHHRPTHK ++
Sbjct: 603 YVVVTDDGIFQLLMHHRPTHKPLN 626

BLAST of Chy8G155010 vs. ExPASy TrEMBL
Match: A0A6J1EDB3 (Threonine dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111433135 PE=3 SV=1)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 544/624 (87.18%), Postives = 574/624 (91.99%), Query Frame = 0

Query: 4   LLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPVILATLSKHAVDIPPN 63
           +L NAPT HP+ RK  L SP    NG+SI IDDNRNR+R+ R P I ATLSKH V+  PN
Sbjct: 3   VLPNAPTPHPLSRKWSLLSPAFLLNGTSIPIDDNRNRIRVNRVPFIQATLSKHTVENLPN 62

Query: 64  SVSNTSVSTPGDALAPTPSPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAMEYLT 123
           SVSNT+V    DAL   P PPRKRV ADSLQFPPGYLGAVPDR GSD+ EDS+DAMEYLT
Sbjct: 63  SVSNTAVVALEDALVAVPLPPRKRVLADSLQFPPGYLGAVPDRLGSDDEEDSLDAMEYLT 122

Query: 124 RILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL 183
           RILGSKVYDVAIESPLQLAP LSER GVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL
Sbjct: 123 RILGSKVYDVAIESPLQLAPMLSERLGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL 182

Query: 184 EKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA 243
           E+GVICSSAGNHAQGVALAA+RLRC+AVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA
Sbjct: 183 ERGVICSSAGNHAQGVALAARRLRCDAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA 242

Query: 244 QAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIA 303
           QAYAKKRSV+EGRTFIPPFDHPDVIAGQGTVGMEIVRQ+K  +HAIFVPVGGGGLIAGIA
Sbjct: 243 QAYAKKRSVDEGRTFIPPFDHPDVIAGQGTVGMEIVRQMKSKLHAIFVPVGGGGLIAGIA 302

Query: 304 AYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKD 363
           AYVKRVSPEVKIIGVEPSDANAMALSLCHGQR+IL+KAGGFADGVAVKEVGEETFRLCK 
Sbjct: 303 AYVKRVSPEVKIIGVEPSDANAMALSLCHGQRIILEKAGGFADGVAVKEVGEETFRLCKG 362

Query: 364 LMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA 423
           LMDGVVLV RDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA
Sbjct: 363 LMDGVVLVGRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA 422

Query: 424 NMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSEKEA 483
           NMNFDKL IVTELANVGRE+EAVL T LPE PGSFK+FCEL+G MNITEFKYRY+SE+EA
Sbjct: 423 NMNFDKLRIVTELANVGREQEAVLVTKLPEKPGSFKRFCELIGAMNITEFKYRYNSEEEA 482

Query: 484 VVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLCRFI 543
           VVLYSVG+HMPSELEEM +RM SSQL TYNLT NDLVKDHLRYLMGG+SNVENEVLCRFI
Sbjct: 483 VVLYSVGMHMPSELEEMMNRMNSSQLETYNLTNNDLVKDHLRYLMGGRSNVENEVLCRFI 542

Query: 544 FPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKHDMGEFHERARRVGYD 603
           FPERPGALEKFLDA SPRWNI+LFHY  +GETGANI VGLQIEK +MGEFHERARRVGYD
Sbjct: 543 FPERPGALEKFLDAFSPRWNISLFHYHGQGETGANIFVGLQIEKPEMGEFHERARRVGYD 602

Query: 604 YVVVTDDGIFQLLMHHRPTHKAID 628
           YVVVTDDGIFQLLMHHRPTHK ++
Sbjct: 603 YVVVTDDGIFQLLMHHRPTHKPLN 626

BLAST of Chy8G155010 vs. NCBI nr
Match: XP_004143291.1 (threonine dehydratase biosynthetic, chloroplastic [Cucumis sativus] >KGN48217.1 hypothetical protein Csa_003876 [Cucumis sativus])

HSP 1 Score: 1239 bits (3206), Expect = 0.0
Identity = 623/630 (98.89%), Postives = 626/630 (99.37%), Query Frame = 0

Query: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPVILATLSKHAVDI 60
           MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPP+ILATLSKHA+DI
Sbjct: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDI 60

Query: 61  PPNSVSNTSVSTPGDALAPTPSPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120
           PPNSVSNTSV TP DALAPTP PPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME
Sbjct: 61  PPNSVSNTSVPTPADALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120

Query: 121 YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK 180
           YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK
Sbjct: 121 YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK 180

Query: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240
           EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY
Sbjct: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240

Query: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300
           DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA
Sbjct: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300

Query: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360
           GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL
Sbjct: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360

Query: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420
           CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT
Sbjct: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420

Query: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480
           SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE
Sbjct: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480

Query: 481 KEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC 540
           KEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC
Sbjct: 481 KEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC 540

Query: 541 RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKHDMGEFHERARRV 600
           RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEK DMGEFHERARRV
Sbjct: 541 RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKPDMGEFHERARRV 600

Query: 601 GYDYVVVTDDGIFQLLMHHRPTHKAIDLLS 630
           GYDYVVVTDDGIFQLLMHHRP+HKAIDLLS
Sbjct: 601 GYDYVVVTDDGIFQLLMHHRPSHKAIDLLS 630

BLAST of Chy8G155010 vs. NCBI nr
Match: XP_008462516.1 (PREDICTED: threonine dehydratase biosynthetic, chloroplastic [Cucumis melo] >KAA0025308.1 threonine dehydratase biosynthetic [Cucumis melo var. makuwa] >TYK07361.1 threonine dehydratase biosynthetic [Cucumis melo var. makuwa])

HSP 1 Score: 1228 bits (3176), Expect = 0.0
Identity = 617/630 (97.94%), Postives = 623/630 (98.89%), Query Frame = 0

Query: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPVILATLSKHAVDI 60
           M+ALLLNAPTSHPIPRKSFLPSPPSFFNG+SISIDDNRNRVRLTRPP I ATLSKHAVDI
Sbjct: 1   MEALLLNAPTSHPIPRKSFLPSPPSFFNGTSISIDDNRNRVRLTRPPFIQATLSKHAVDI 60

Query: 61  PPNSVSNTSVSTPGDALAPTPSPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120
           PPNSVSNTS+ TPGDALAPTP PPR+RVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME
Sbjct: 61  PPNSVSNTSIPTPGDALAPTPLPPRRRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120

Query: 121 YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK 180
           YLTRILGSKVYDVAIESPLQLAPTLSER G NIWLKREDLQPVFSFKLRGAYNMMAKLP+
Sbjct: 121 YLTRILGSKVYDVAIESPLQLAPTLSERLGNNIWLKREDLQPVFSFKLRGAYNMMAKLPQ 180

Query: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240
           EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY
Sbjct: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240

Query: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300
           DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA
Sbjct: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300

Query: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360
           GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL
Sbjct: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360

Query: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420
           CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT
Sbjct: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420

Query: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480
           SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE
Sbjct: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480

Query: 481 KEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC 540
           KEAVVLYSVGIHMPSELEEMK+RMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC
Sbjct: 481 KEAVVLYSVGIHMPSELEEMKNRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLC 540

Query: 541 RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKHDMGEFHERARRV 600
           RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEK DMGEFHERARRV
Sbjct: 541 RFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKPDMGEFHERARRV 600

Query: 601 GYDYVVVTDDGIFQLLMHHRPTHKAIDLLS 630
           GYDYVVVTDDGIFQLLMHHRPTHKAIDLLS
Sbjct: 601 GYDYVVVTDDGIFQLLMHHRPTHKAIDLLS 630

BLAST of Chy8G155010 vs. NCBI nr
Match: XP_038881616.1 (threonine dehydratase biosynthetic, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1162 bits (3006), Expect = 0.0
Identity = 587/632 (92.88%), Postives = 609/632 (96.36%), Query Frame = 0

Query: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNG-SSISIDDNRNRVRLTRPPVILATLSKHAVD 60
           M+ALLLNAPTSHPI R+S L S PSF NG +SIS+DDNR+R+RLTRPP I ATLSKH VD
Sbjct: 1   MEALLLNAPTSHPIARRSLLLSSPSFLNGGASISLDDNRSRIRLTRPPFIQATLSKHTVD 60

Query: 61  IPPNSVSNTSVSTPGDALAPTPSPPRKRVSADSLQFPPGYLGAVPDRSGSDN-GEDSVDA 120
           IPPNSV+NTSV T GDALA TP PPRKRVSADSLQFPPGYLGAVPDRSGSD+ GEDS++A
Sbjct: 61  IPPNSVTNTSVPTLGDALAATPLPPRKRVSADSLQFPPGYLGAVPDRSGSDDDGEDSLNA 120

Query: 121 MEYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKL 180
           MEYLTRILGSKVYDVAIESPLQLAPTLSER GVNIWLKREDLQPVFSFKLRGAYNMMAKL
Sbjct: 121 MEYLTRILGSKVYDVAIESPLQLAPTLSERLGVNIWLKREDLQPVFSFKLRGAYNMMAKL 180

Query: 181 PKEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGD 240
           PKEQL+KGVICSSAGNHAQGVALAAKRLRCNAVIAMP+TTP+IKWQSV+KLGATVVLVGD
Sbjct: 181 PKEQLDKGVICSSAGNHAQGVALAAKRLRCNAVIAMPLTTPQIKWQSVKKLGATVVLVGD 240

Query: 241 SYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGL 300
           SYDEAQAYAKKR VEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGP+HAIFVPVGGGGL
Sbjct: 241 SYDEAQAYAKKRGVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPLHAIFVPVGGGGL 300

Query: 301 IAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETF 360
           IAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHG+R+ILDKAGGFADGVAVKEVGEETF
Sbjct: 301 IAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGERIILDKAGGFADGVAVKEVGEETF 360

Query: 361 RLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVV 420
           RLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYY LKGENVVV
Sbjct: 361 RLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYDLKGENVVV 420

Query: 421 ITSGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYS 480
           ITSGANMNFDKLSIVTELANVGREKEAVLATILPE PGSFK+FCELVGPMNITEFKYRYS
Sbjct: 421 ITSGANMNFDKLSIVTELANVGREKEAVLATILPEIPGSFKRFCELVGPMNITEFKYRYS 480

Query: 481 SEKEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEV 540
           SEKEAVVLYSVGIHMPSELEEMK+RMES+QL TYNLTKNDLVKDHLRYLMGGKSNVENEV
Sbjct: 481 SEKEAVVLYSVGIHMPSELEEMKNRMESAQLETYNLTKNDLVKDHLRYLMGGKSNVENEV 540

Query: 541 LCRFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKHDMGEFHERAR 600
           LCRFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEK DM EFHER R
Sbjct: 541 LCRFIFPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKSDMSEFHERVR 600

Query: 601 RVGYDYVVVTDDGIFQLLMHHRPTHKAIDLLS 630
           RVGYDYVVVTDDGIFQLLMHHRP+HKA +LLS
Sbjct: 601 RVGYDYVVVTDDGIFQLLMHHRPSHKAANLLS 632

BLAST of Chy8G155010 vs. NCBI nr
Match: XP_022977238.1 (threonine dehydratase biosynthetic, chloroplastic-like [Cucurbita maxima] >XP_022977239.1 threonine dehydratase biosynthetic, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1091 bits (2822), Expect = 0.0
Identity = 547/624 (87.66%), Postives = 579/624 (92.79%), Query Frame = 0

Query: 4   LLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPVILATLSKHAVDIPPN 63
           +LLNAPTSHP+ RK  L SP  F NG+SI IDDNRNR+R+TR P I ATLSKH V+  PN
Sbjct: 3   VLLNAPTSHPLSRKWSLLSPAFFLNGTSIPIDDNRNRIRVTRVPFIQATLSKHTVENLPN 62

Query: 64  SVSNTSVSTPGDALAPTPSPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAMEYLT 123
           SVSNT+V    DALA  P PPRKRV ADSLQFPPGYLGAVPDR GSD+ EDS+DAMEYLT
Sbjct: 63  SVSNTAVVALEDALAAVPLPPRKRVLADSLQFPPGYLGAVPDRLGSDDEEDSLDAMEYLT 122

Query: 124 RILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL 183
           RILGSKVYDVAIESPLQLAP LSER GVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL
Sbjct: 123 RILGSKVYDVAIESPLQLAPMLSERLGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL 182

Query: 184 EKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA 243
           E+GVICSSAGNHAQGVALAA+RLRC+AVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA
Sbjct: 183 ERGVICSSAGNHAQGVALAARRLRCDAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA 242

Query: 244 QAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIA 303
           QAYAKKRS++EGRTFIPPFDHPDVIAGQGTVGMEIVRQ+K  +HAIFVPVGGGGLIAGIA
Sbjct: 243 QAYAKKRSIDEGRTFIPPFDHPDVIAGQGTVGMEIVRQMKSKLHAIFVPVGGGGLIAGIA 302

Query: 304 AYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKD 363
           AYVKRVSPEVKIIGVEPSDANAMALSLCHGQR+IL+KAGGFADGVAVKEVGEETFRLCK 
Sbjct: 303 AYVKRVSPEVKIIGVEPSDANAMALSLCHGQRIILEKAGGFADGVAVKEVGEETFRLCKG 362

Query: 364 LMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA 423
           LMDGVVLV RDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA
Sbjct: 363 LMDGVVLVGRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA 422

Query: 424 NMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSEKEA 483
           NMNFDKL IVTELANVGRE+EAVL T LPE PGSFK+FCEL+G MNITEFKYRY+SEKEA
Sbjct: 423 NMNFDKLRIVTELANVGREQEAVLVTKLPEKPGSFKRFCELIGAMNITEFKYRYNSEKEA 482

Query: 484 VVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLCRFI 543
           VVLYSVG+HMPSELEEM +RM SSQL TYNLT NDLVKDHLRYLMGG+SNVENEVLCRFI
Sbjct: 483 VVLYSVGMHMPSELEEMMNRMNSSQLETYNLTNNDLVKDHLRYLMGGRSNVENEVLCRFI 542

Query: 544 FPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKHDMGEFHERARRVGYD 603
           FPERPGALEKFLDA SPRWNI+LFHY  +GETGANI VGLQ+EK ++GEFHERARRVGYD
Sbjct: 543 FPERPGALEKFLDAFSPRWNISLFHYHGQGETGANIFVGLQMEKPELGEFHERARRVGYD 602

Query: 604 YVVVTDDGIFQLLMHHRPTHKAID 627
           YVVVTDDGIFQLLMHHRPTHK ++
Sbjct: 603 YVVVTDDGIFQLLMHHRPTHKPLN 626

BLAST of Chy8G155010 vs. NCBI nr
Match: XP_023544869.1 (threonine dehydratase biosynthetic, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1087 bits (2810), Expect = 0.0
Identity = 546/624 (87.50%), Postives = 577/624 (92.47%), Query Frame = 0

Query: 4   LLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPVILATLSKHAVDIPPN 63
           +LLNAPTSHP+ RK  L SP  F NG+SI IDDNRN +R+TR P I ATLSKH V+  PN
Sbjct: 3   VLLNAPTSHPLSRKWSLLSPAFFLNGTSIPIDDNRNGIRVTRVPFIQATLSKHTVENLPN 62

Query: 64  SVSNTSVSTPGDALAPTPSPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAMEYLT 123
           SVSNT+V    DAL   P PPRKRV ADSLQFPPGYLGAVPDR GSD+ EDS+DAMEYLT
Sbjct: 63  SVSNTAVVALEDALVAVPLPPRKRVLADSLQFPPGYLGAVPDRLGSDDEEDSLDAMEYLT 122

Query: 124 RILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL 183
           RILGSKVYDVAIESPLQLAP LSER GVNIWLKREDLQPVFSFKLRGAYNMMAKLPKE+L
Sbjct: 123 RILGSKVYDVAIESPLQLAPMLSERLGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKERL 182

Query: 184 EKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA 243
           E+GVICSSAGNHAQGVALAA+RLRC+AVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA
Sbjct: 183 ERGVICSSAGNHAQGVALAARRLRCDAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA 242

Query: 244 QAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIA 303
           QAYAKKRSV+EGRTFIPPFDHPDVIAGQGTVGMEIVRQ+K  +HAIFVPVGGGGLIAGIA
Sbjct: 243 QAYAKKRSVDEGRTFIPPFDHPDVIAGQGTVGMEIVRQMKSKLHAIFVPVGGGGLIAGIA 302

Query: 304 AYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKD 363
           AYVKRVSPEVKIIGVEPSDANAMALSLCHGQR+IL+KAGGFADGVAVKEVGEETFRLCK 
Sbjct: 303 AYVKRVSPEVKIIGVEPSDANAMALSLCHGQRIILEKAGGFADGVAVKEVGEETFRLCKG 362

Query: 364 LMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA 423
           LMDGVVLV RDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA
Sbjct: 363 LMDGVVLVGRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA 422

Query: 424 NMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSEKEA 483
           NMNFDKL IVTELANVGRE+EAVL T LPE PGSFK+FCEL+G MNITEFKYRY+SE+EA
Sbjct: 423 NMNFDKLRIVTELANVGREQEAVLVTKLPEKPGSFKRFCELIGAMNITEFKYRYNSEEEA 482

Query: 484 VVLYSVGIHMPSELEEMKDRMESSQLPTYNLTKNDLVKDHLRYLMGGKSNVENEVLCRFI 543
           VVLYSVG+HMPSELEEM +RM SSQL TYNLT NDLVKDHLRYLMGG+SNVENEVLCRFI
Sbjct: 483 VVLYSVGMHMPSELEEMMNRMNSSQLETYNLTNNDLVKDHLRYLMGGRSNVENEVLCRFI 542

Query: 544 FPERPGALEKFLDALSPRWNITLFHYQSKGETGANILVGLQIEKHDMGEFHERARRVGYD 603
           FPERPGALEKFLDA SPRWNI+LFHY  +GETGANI VGLQIEK +MGEFHERARRVGYD
Sbjct: 543 FPERPGALEKFLDAFSPRWNISLFHYHGQGETGANIFVGLQIEKPEMGEFHERARRVGYD 602

Query: 604 YVVVTDDGIFQLLMHHRPTHKAID 627
           YVVVTDDGIFQLLMHHRPTHK ++
Sbjct: 603 YVVVTDDGIFQLLMHHRPTHKPLN 626

BLAST of Chy8G155010 vs. TAIR 10
Match: AT3G10050.1 (L-O-methylthreonine resistant 1 )

HSP 1 Score: 842.4 bits (2175), Expect = 2.3e-244
Identity = 425/583 (72.90%), Postives = 488/583 (83.70%), Query Frame = 0

Query: 38  RNRVRLTRPPVILAT--LSKHAVDIPPNSVSNTSVSTPGDALAPTPSPPRKRVSADSLQF 97
           R+ +     PV+  T   S+  + +   S   TS++ P     P    PR +VS +SLQ+
Sbjct: 14  RSHIHRPSKPVVGFTHFSSRSRIAVAVLSRDETSMTPP----PPKLPLPRLKVSPNSLQY 73

Query: 98  PPGYLGAVPDRSGSDNGEDSVDAMEYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWL 157
           P GYLGAVP+R+         +AMEYLT IL +KVYD+AIESPLQLA  LS+R GV ++L
Sbjct: 74  PAGYLGAVPERTNEAENGSIAEAMEYLTNILSTKVYDIAIESPLQLAKKLSKRLGVRMYL 133

Query: 158 KREDLQPVFSFKLRGAYNMMAKLPKEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMP 217
           KREDLQPVFSFKLRGAYNMM KLP +QL KGVICSSAGNHAQGVAL+A +L C AVI MP
Sbjct: 134 KREDLQPVFSFKLRGAYNMMVKLPADQLAKGVICSSAGNHAQGVALSASKLGCTAVIVMP 193

Query: 218 VTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVG 277
           VTTPEIKWQ+V+ LGATVVL GDSYD+AQA+AK R+ EEG TFIPPFDHPDVIAGQGTVG
Sbjct: 194 VTTPEIKWQAVENLGATVVLFGDSYDQAQAHAKIRAEEEGLTFIPPFDHPDVIAGQGTVG 253

Query: 278 MEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQR 337
           MEI RQ KGP+HAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEP+DANAMALSL HG+R
Sbjct: 254 MEITRQAKGPLHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPADANAMALSLHHGER 313

Query: 338 VILDKAGGFADGVAVKEVGEETFRLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAG 397
           VILD+ GGFADGVAVKEVGEETFR+ ++LMDGVVLV+RDAICASIKDMFEEKR+ILEPAG
Sbjct: 314 VILDQVGGFADGVAVKEVGEETFRISRNLMDGVVLVTRDAICASIKDMFEEKRNILEPAG 373

Query: 398 ALSLAGAEAYCKYYGLKGENVVVITSGANMNFDKLSIVTELANVGREKEAVLATILPETP 457
           AL+LAGAEAYCKYYGLK  NVV ITSGANMNFDKL IVTELANVGR++EAVLAT++PE P
Sbjct: 374 ALALAGAEAYCKYYGLKDVNVVAITSGANMNFDKLRIVTELANVGRQQEAVLATLMPEKP 433

Query: 458 GSFKKFCELVGPMNITEFKYRYSSEKEAVVLYSVGIHMPSELEEMKDRMESSQLPTYNLT 517
           GSFK+FCELVGPMNI+EFKYR SSEKEAVVLYSVG+H   EL+ ++ RMESSQL T NLT
Sbjct: 434 GSFKQFCELVGPMNISEFKYRCSSEKEAVVLYSVGVHTAGELKALQKRMESSQLKTVNLT 493

Query: 518 KNDLVKDHLRYLMGGKSNVENEVLCRFIFPERPGALEKFLDALSPRWNITLFHYQSKGET 577
            +DLVKDHLRYLMGG+S V +EVLCRF FPERPGAL  FLD+ SPRWNITLFHY+ +GET
Sbjct: 494 TSDLVKDHLRYLMGGRSTVGDEVLCRFTFPERPGALMNFLDSFSPRWNITLFHYRGQGET 553

Query: 578 GANILVGLQIEKHDMGEFHERARRVGYDYVVVTDDGIFQLLMH 619
           GAN+LVG+Q+ + +M EF  RA+ +GYDY +V+DD  F+LLMH
Sbjct: 554 GANVLVGIQVPEQEMEEFKNRAKALGYDYFLVSDDDYFKLLMH 592

BLAST of Chy8G155010 vs. TAIR 10
Match: AT4G11640.1 (serine racemase )

HSP 1 Score: 156.0 bits (393), Expect = 1.0e-37
Identity = 96/297 (32.32%), Postives = 161/297 (54.21%), Query Frame = 0

Query: 137 SPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQLEKGVICSSAGNHA 196
           +P+  + +L+   G +++ K E LQ   +FK RGA N +  L  EQ  KGV+  S+GNHA
Sbjct: 29  TPVLTSESLNSISGRSLFFKCECLQKGGAFKFRGACNAVLSLDAEQAAKGVVTHSSGNHA 88

Query: 197 QGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSVEEGR 256
             ++LAAK     A I +P   P+ K  +V + G  V+    +    +  A K   E G 
Sbjct: 89  AALSLAAKIQGIPAYIVVPKGAPKCKVDNVIRYGGKVIWSEATMSSREEIASKVLQETGS 148

Query: 257 TFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKII 316
             I P++   +I+GQGT+ +E++ QI+  + AI VP+ GGGLI+G+A   K + P ++II
Sbjct: 149 VLIHPYNDGRIISGQGTIALELLEQIQ-EIDAIVVPISGGGLISGVALAAKSIKPSIRII 208

Query: 317 GVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKDLMDGVVLVSRDAI 376
             EP  A+  A S   G+ + L      ADG+    +G+ T+ + +DL+D VV +    I
Sbjct: 209 AAEPKGADDAAQSKVAGKIITLPVTNTIADGLRA-SLGDLTWPVVRDLVDDVVTLEECEI 268

Query: 377 CASIKDMFEEKRSILEPAGALSLAG--AEAYCKYYGLKG-ENVVVITSGANMNFDKL 431
             ++K  +E  +  +EP+GA+ LA   + ++      +  +N+ ++ SG N++   L
Sbjct: 269 IEAMKMCYEILKVSVEPSGAIGLAAVLSNSFRNNPSCRDCKNIGIVLSGGNVDLGSL 323

BLAST of Chy8G155010 vs. TAIR 10
Match: AT3G61440.1 (cysteine synthase C1 )

HSP 1 Score: 65.1 bits (157), Expect = 2.3e-10
Identity = 74/320 (23.12%), Postives = 131/320 (40.94%), Query Frame = 0

Query: 136 ESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL----EKGVICSS 195
           ++PL     ++E     +  K+E  QP  S K R A  M+A   K++L    +  +I  +
Sbjct: 59  KTPLVFLNKVTEGCEAYVAAKQEHFQPTCSIKDRPAIAMIADAEKKKLIIPGKTTLIEPT 118

Query: 196 AGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLV----GDSYDEAQAYA 255
           +GN    +A  A       ++ MP  T   +  +++  GA +VL     G      +AY 
Sbjct: 119 SGNMGISLAFMAAMKGYRIIMTMPSYTSLERRVTMRSFGAELVLTDPAKGMGGTVKKAYD 178

Query: 256 KKRSVEEGRTFIPPFDHP-DVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYV 315
              S  +       F +P +      T G EI     G V    + +G GG ++G+  Y+
Sbjct: 179 LLDSTPDA-FMCQQFANPANTQIHFDTTGPEIWEDTLGNVDIFVMGIGSGGTVSGVGRYL 238

Query: 316 KRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKDLMD 375
           K  +P VKI GVEP+++N            IL+        +    VG +   L  D+M+
Sbjct: 239 KSKNPNVKIYGVEPAESN------------ILNGGKPGPHAITGNGVGFKPEILDMDVME 298

Query: 376 GVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGANMN 435
            V+ VS +      +++  ++  ++  +   +   A    K    KG+ +V I +     
Sbjct: 299 SVLEVSSEDAIKMARELALKEGLMVGISSGANTVAAIRLAKMPENKGKLIVTIHASFGER 358

Query: 436 FDKLSIVTELANVGREKEAV 447
           +    +  EL     E + V
Sbjct: 359 YLSSVLFDELRKEAEEMKPV 365

BLAST of Chy8G155010 vs. TAIR 10
Match: AT2G43750.1 (O-acetylserine (thiol) lyase B )

HSP 1 Score: 62.0 bits (149), Expect = 2.0e-09
Identity = 45/176 (25.57%), Postives = 81/176 (46.02%), Query Frame = 0

Query: 156 KREDLQPVFSFKLRGAYNMMAKLPKEQL----EKGVICSSAGNHAQGVALAAKRLRCNAV 215
           K E ++P  S K R  Y+M+    ++ L    +  ++ S++GN   G+A  A       +
Sbjct: 105 KLEIMEPCCSVKDRIGYSMITDAEEKGLITPGKSVLVESTSGNTGIGLAFIAASKGYKLI 164

Query: 216 IAMPVTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSV---EEGRTFIPPFDHP-DV 275
           + MP +    +   ++  GA +VL   +     A  K   +         +  FD+P + 
Sbjct: 165 LTMPASMSLERRVLLRAFGAELVLTEPAKGMTGAIQKAEEILKKTPNSYMLQQFDNPANP 224

Query: 276 IAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDA 324
                T G EI    +G +  +   +G GG I G+  ++K   PE+K+IGVEP+++
Sbjct: 225 KIHYETTGPEIWEDTRGKIDILVAGIGTGGTITGVGRFIKERKPELKVIGVEPTES 280

BLAST of Chy8G155010 vs. TAIR 10
Match: AT2G43750.2 (O-acetylserine (thiol) lyase B )

HSP 1 Score: 62.0 bits (149), Expect = 2.0e-09
Identity = 45/176 (25.57%), Postives = 81/176 (46.02%), Query Frame = 0

Query: 156 KREDLQPVFSFKLRGAYNMMAKLPKEQL----EKGVICSSAGNHAQGVALAAKRLRCNAV 215
           K E ++P  S K R  Y+M+    ++ L    +  ++ S++GN   G+A  A       +
Sbjct: 105 KLEIMEPCCSVKDRIGYSMITDAEEKGLITPGKSVLVESTSGNTGIGLAFIAASKGYKLI 164

Query: 216 IAMPVTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSV---EEGRTFIPPFDHP-DV 275
           + MP +    +   ++  GA +VL   +     A  K   +         +  FD+P + 
Sbjct: 165 LTMPASMSLERRVLLRAFGAELVLTEPAKGMTGAIQKAEEILKKTPNSYMLQQFDNPANP 224

Query: 276 IAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDA 324
                T G EI    +G +  +   +G GG I G+  ++K   PE+K+IGVEP+++
Sbjct: 225 KIHYETTGPEIWEDTRGKIDILVAGIGTGGTITGVGRFIKERKPELKVIGVEPTES 280

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZSS63.3e-24372.90Threonine dehydratase biosynthetic, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
A0FKE69.6e-23569.97Threonine dehydratase 1 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4... [more]
Q9AXU44.1e-19362.59Threonine dehydratase OS=Nicotiana attenuata OX=49451 GN=TD PE=1 SV=1[more]
P253068.9e-15654.64Threonine dehydratase 2 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4... [more]
P536072.9e-14652.39L-threonine dehydratase biosynthetic IlvA OS=Burkholderia multivorans (strain AT... [more]
Match NameE-valueIdentityDescription
A0A0A0KII40.0e+0098.89Threonine dehydratase OS=Cucumis sativus OX=3659 GN=Csa_6G449240 PE=3 SV=1[more]
A0A5A7SGN70.0e+0097.94Threonine dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold20... [more]
A0A1S3CHM70.0e+0097.94Threonine dehydratase OS=Cucumis melo OX=3656 GN=LOC103500854 PE=3 SV=1[more]
A0A6J1IPE00.0e+0087.66Threonine dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111477608 PE=3 SV=1[more]
A0A6J1EDB30.0e+0087.18Threonine dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111433135 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004143291.10.098.89threonine dehydratase biosynthetic, chloroplastic [Cucumis sativus] >KGN48217.1 ... [more]
XP_008462516.10.097.94PREDICTED: threonine dehydratase biosynthetic, chloroplastic [Cucumis melo] >KAA... [more]
XP_038881616.10.092.88threonine dehydratase biosynthetic, chloroplastic-like isoform X1 [Benincasa his... [more]
XP_022977238.10.087.66threonine dehydratase biosynthetic, chloroplastic-like [Cucurbita maxima] >XP_02... [more]
XP_023544869.10.087.50threonine dehydratase biosynthetic, chloroplastic-like [Cucurbita pepo subsp. pe... [more]
Match NameE-valueIdentityDescription
AT3G10050.12.3e-24472.90L-O-methylthreonine resistant 1 [more]
AT4G11640.11.0e-3732.32serine racemase [more]
AT3G61440.12.3e-1023.13cysteine synthase C1 [more]
AT2G43750.12.0e-0925.57O-acetylserine (thiol) lyase B [more]
AT2G43750.22.0e-0925.57O-acetylserine (thiol) lyase B [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038110Threonine dehydratase, ACT-like domain superfamilyGENE3D3.40.1020.10Biosynthetic Threonine Deaminase; Domain 3coord: 444..618
e-value: 8.4E-63
score: 213.1
IPR005787Threonine dehydratase, biosyntheticTIGRFAMTIGR01124TIGR01124coord: 120..616
e-value: 1.9E-201
score: 667.9
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 138..416
e-value: 9.0E-109
score: 365.0
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 170..261
e-value: 9.0E-109
score: 365.0
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 120..472
IPR001721Threonine dehydratase, ACT-like domainPFAMPF00585Thr_dehydrat_Ccoord: 529..615
e-value: 4.7E-23
score: 80.8
coord: 435..524
e-value: 7.8E-23
score: 80.1
IPR001721Threonine dehydratase, ACT-like domainPROSITEPS51672ACT_LIKEcoord: 538..609
score: 15.676129
IPR001721Threonine dehydratase, ACT-like domainPROSITEPS51672ACT_LIKEcoord: 445..516
score: 15.598662
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 134..422
e-value: 6.2E-82
score: 275.3
NoneNo IPR availablePIRSRPIRSR006278-2PIRSR006278-2coord: 150..320
e-value: 5.6E-5
score: 20.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..76
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..91
NoneNo IPR availablePANTHERPTHR48078THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATEDcoord: 52..617
NoneNo IPR availablePANTHERPTHR48078:SF1THREONINE DEHYDRATASEcoord: 52..617
NoneNo IPR availableCDDcd01562Thr-dehydcoord: 122..426
e-value: 4.66664E-144
score: 418.429
NoneNo IPR availableCDDcd04907ACT_ThrD-I_2coord: 537..617
e-value: 4.70112E-32
score: 116.883
NoneNo IPR availableCDDcd04906ACT_ThrD-I_1coord: 444..527
e-value: 9.43277E-36
score: 127.279
NoneNo IPR availableSUPERFAMILY55021ACT-likecoord: 525..617
IPR000634Serine/threonine dehydratase, pyridoxal-phosphate-binding sitePROSITEPS00165DEHYDRATASE_SER_THRcoord: 158..171

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy8G155010.1Chy8G155010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009097 isoleucine biosynthetic process
biological_process GO:0006567 threonine catabolic process
biological_process GO:0006520 cellular amino acid metabolic process
molecular_function GO:0004794 L-threonine ammonia-lyase activity
molecular_function GO:0030170 pyridoxal phosphate binding