Chy8G154980 (gene) Cucumber (hystrix) v1

Overview


Name	Chy8G154980
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Threonine dehydratase
Location	chrH08: 16338647 .. 16340578 (+)
RNA-Seq Expression	Chy8G154980
Synteny	Chy8G154980

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATTGGACGTCAAATGAGAGGTCCATTACATGCAATTTTTGTCCCGGTTGGGGGTGGCGGTCTTCTAGCCGGTGTTGCTTCATTTTACAAGCTAGTTTTTCCGAAGGTAAAAGCTTAGTCGTTCCTGTCTGTACTAAACATTTAGAAAAGTAATTAATTCCAAACAGACTCTTGCTATATTTCTACCTGTAATACACAGGTAAAGATTATTGGGGTGGAGCCAAATGATGCAAATTCCATGGCATCTGCATTACATAATGATCAAGTAGTAAAATTAGAAACAGTTGGAACTTTTGTAGATGGTGTGGCTGTTAAACAAGTTGGGGATGAAAATTTTCGAATTAGTAGAGAACTCATTGATGGTATTGTTCTTGTTGATAAAGACGCCATAGCCGCTTGCATAAAGGTTTTTACCTTTCTCTTCTTACGCTATTTTGTTTTGACCCCTGCTGATCCCCTGACATCGGTTTCCTTAAAAAAAAATGATAATTAACAAGTGTTTTGTTGTTCATATAATTAATAGGATATGTTTGAGGACACAATATAATTAATAGGATATGTTTGAGGACACAAGGAGCATATTGGAACCAGCAGGGGCACTTTCAATTGCAGGAGCAAAAGCATATTGTCAACATAATAAAATAAAAGGAGTGAAGGCAGTAACAAGTGGTGCAAATATGAACTTTGATCAATTAGGTAGCATTGCTGATAATGCTGATTCTGGTAATCAAACTGAGGCTACTTTTGCAACTATACTCCCTGAAAAACCTGGAAGCTTAAGAACATTTTCTGACTTGGTAATTTTTCATTTTATTTCAATCTTTAATTAATTACATTAAATATCAATTAGTTTTAATCTTCATATATATCATCATTTTTATTTAATTACATTGATAATATAATTGATATTCAAAATATGCAGATGGGATCAAGAAGCGTTACCGAATTTAAGTACCGATTCAACTCCGAAAAGGATGCAATTGTGCTTTATAGGTAAATTAATTATTTAAAGTTTGATTTGTTTGTGTTGTTAATTATTTTTAATTTCATCATGTTGGAAGTATTAGCAAATAAAGTATAGTACAATATGCAAAAGAATCTGCTAAGTAATAAAATTTAGATTCAACACACGTGATTATATGATAGTCTATATTATTAATAGTAATATTGGACTTTATTAATAATATATATAGAGTTCTGTCGCTAATAGGATCAGACTTTATTTGTTACACTTATAATTAATAATTAAGTTGTGTGATTTTTTGTTGGTAGTGTTGGGGTGAAGGTGGCATCAGAGCTGGGAGAAGTGAAGAAAAAGACAGAATCTTCACCATTTGAAACTTATGATCTCACAAAGAATGAACTTGTTAAGGATCACTTGCGTTACATGGTAATTAATTAATTAATTATTATTATTGCTCACCCTTTTTGCTGTGGCCTATTGTTATATTTGAGCTTTTAATTACATTCTAGAACTATAAGTTTTAGTGTCTAAGATTGGTCTCTCTAACTTACTTAAGTTTTGCAGATGGGAGGTAGATCAAGCATTCCAAATGAAGTTTTCTATCGTTTTACCCTCCCAAAAACATCAGGAGCTCTATTACAATTCCTAGATGCTTTCAGTCCACGTTGGAACATTAGCTTAATTCACTATCGTCGACAGGTATAACGCTATGACCTTTAATTAATTGTTAAAGTATTGATATATATAACTAAATTTAGGATCACATAATTTAATTTGCTAAGTGAAGTATCAAATATATGTTTGGTGTTTGTGCAGGGTATAATTAGTGCAGATGTATTGGTTGGGCTTCAGATTGAAGGATCAGAAGAGGCTGAATTCCATGAAAGTGCTAGAAAGCTTGGATTTGATTATGTTGTTGTTGCTAATGATCCAGCTTCAAAGCTTCTAACACAAGTAGTGTAA

mRNA sequence

ATGGAAATTGGACGTCAAATGAGAGGTCCATTACATGCAATTTTTGTCCCGGTTGGGGGTGGCGGTCTTCTAGCCGGTGTTGCTTCATTTTACAAGCTAGTTTTTCCGAAGGTAAAGATTATTGGGGTGGAGCCAAATGATGCAAATTCCATGGCATCTGCATTACATAATGATCAAGTAGTAAAATTAGAAACAGTTGGAACTTTTGTAGATGGTGTGGCTGTTAAACAAGTTGGGGATGAAAATTTTCGAATTAGTAGAGAACTCATTGATGGTATTGTTCTTGTTGATAAAGACGCCATAGCCGCTTGCATAAAGGATATGTTTGAGGACACAAGGAGCATATTGGAACCAGCAGGGGCACTTTCAATTGCAGGAGCAAAAGCATATTGTCAACATAATAAAATAAAAGGAGTGAAGGCAGTAACAAGTGGTGCAAATATGAACTTTGATCAATTAGGTAGCATTGCTGATAATGCTGATTCTGGTAATCAAACTGAGGCTACTTTTGCAACTATACTCCCTGAAAAACCTGGAAGCTTAAGAACATTTTCTGACTTGATGGGATCAAGAAGCGTTACCGAATTTAAGTACCGATTCAACTCCGAAAAGGATGCAATTGTGCTTTATAGTGTTGGGGTGAAGGTGGCATCAGAGCTGGGAGAAGTGAAGAAAAAGACAGAATCTTCACCATTTGAAACTTATGATCTCACAAAGAATGAACTTGTTAAGGATCACTTGCGTTACATGATGGGAGGTAGATCAAGCATTCCAAATGAAGTTTTCTATCGTTTTACCCTCCCAAAAACATCAGGAGCTCTATTACAATTCCTAGATGCTTTCAGTCCACGTTGGAACATTAGCTTAATTCACTATCGTCGACAGGGTATAATTAGTGCAGATGTATTGGTTGGGCTTCAGATTGAAGGATCAGAAGAGGCTGAATTCCATGAAAGTGCTAGAAAGCTTGGATTTGATTATGTTGTTGTTGCTAATGATCCAGCTTCAAAGCTTCTAACACAAGTAGTGTAA

Coding sequence (CDS)

ATGGAAATTGGACGTCAAATGAGAGGTCCATTACATGCAATTTTTGTCCCGGTTGGGGGTGGCGGTCTTCTAGCCGGTGTTGCTTCATTTTACAAGCTAGTTTTTCCGAAGGTAAAGATTATTGGGGTGGAGCCAAATGATGCAAATTCCATGGCATCTGCATTACATAATGATCAAGTAGTAAAATTAGAAACAGTTGGAACTTTTGTAGATGGTGTGGCTGTTAAACAAGTTGGGGATGAAAATTTTCGAATTAGTAGAGAACTCATTGATGGTATTGTTCTTGTTGATAAAGACGCCATAGCCGCTTGCATAAAGGATATGTTTGAGGACACAAGGAGCATATTGGAACCAGCAGGGGCACTTTCAATTGCAGGAGCAAAAGCATATTGTCAACATAATAAAATAAAAGGAGTGAAGGCAGTAACAAGTGGTGCAAATATGAACTTTGATCAATTAGGTAGCATTGCTGATAATGCTGATTCTGGTAATCAAACTGAGGCTACTTTTGCAACTATACTCCCTGAAAAACCTGGAAGCTTAAGAACATTTTCTGACTTGATGGGATCAAGAAGCGTTACCGAATTTAAGTACCGATTCAACTCCGAAAAGGATGCAATTGTGCTTTATAGTGTTGGGGTGAAGGTGGCATCAGAGCTGGGAGAAGTGAAGAAAAAGACAGAATCTTCACCATTTGAAACTTATGATCTCACAAAGAATGAACTTGTTAAGGATCACTTGCGTTACATGATGGGAGGTAGATCAAGCATTCCAAATGAAGTTTTCTATCGTTTTACCCTCCCAAAAACATCAGGAGCTCTATTACAATTCCTAGATGCTTTCAGTCCACGTTGGAACATTAGCTTAATTCACTATCGTCGACAGGGTATAATTAGTGCAGATGTATTGGTTGGGCTTCAGATTGAAGGATCAGAAGAGGCTGAATTCCATGAAAGTGCTAGAAAGCTTGGATTTGATTATGTTGTTGTTGCTAATGATCCAGCTTCAAAGCTTCTAACACAAGTAGTGTAA

Protein sequence

MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQVVKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAGALSIAGAKAYCQHNKIKGVKAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKPGSLRTFSDLMGSRSVTEFKYRFNSEKDAIVLYSVGVKVASELGEVKKKTESSPFETYDLTKNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGIISADVLVGLQIEGSEEAEFHESARKLGFDYVVVANDPASKLLTQVV*

Homology

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BLAST of Chy8G154980 vs. ExPASy Swiss-Prot
Match: Q9ZSS6 (Threonine dehydratase biosynthetic, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OMR1 PE=1 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.1e-112
Identity = 203/341 (59.53%), Postives = 266/341 (78.01%), Query Frame = 0


Query: 1   MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQV 60
           MEI RQ +GPLHAIFVPVGGGGL+AG+A++ K V P+VKIIGVEP DAN+MA +LH+ + 
Sbjct: 250 MEITRQAKGPLHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPADANAMALSLHHGER 309

Query: 61  VKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 120
           V L+ VG F DGVAVK+VG+E FRISR L+DG+VLV +DAI A IKDMFE+ R+ILEPAG
Sbjct: 310 VILDQVGGFADGVAVKEVGEETFRISRNLMDGVVLVTRDAICASIKDMFEEKRNILEPAG 369

Query: 121 ALSIAGAKAYCQHNKIKGVK--AVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 180
           AL++AGA+AYC++  +K V   A+TSGANMNFD+L  + + A+ G Q EA  AT++PEKP
Sbjct: 370 ALALAGAEAYCKYYGLKDVNVVAITSGANMNFDKLRIVTELANVGRQQEAVLATLMPEKP 429

Query: 181 GSLRTFSDLMGSRSVTEFKYRFNSEKDAIVLYSVGVKVASELGEVKKKTESSPFETYDLT 240
           GS + F +L+G  +++EFKYR +SEK+A+VLYSVGV  A EL  ++K+ ESS  +T +LT
Sbjct: 430 GSFKQFCELVGPMNISEFKYRCSSEKEAVVLYSVGVHTAGELKALQKRMESSQLKTVNLT 489

Query: 241 KNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGII 300
            ++LVKDHLRY+MGGRS++ +EV  RFT P+  GAL+ FLD+FSPRWNI+L HYR QG  
Sbjct: 490 TSDLVKDHLRYLMGGRSTVGDEVLCRFTFPERPGALMNFLDSFSPRWNITLFHYRGQGET 549

Query: 301 SADVLVGLQIEGSEEAEFHESARKLGFDYVVVANDPASKLL 340
            A+VLVG+Q+   E  EF   A+ LG+DY +V++D   KLL
Sbjct: 550 GANVLVGIQVPEQEMEEFKNRAKALGYDYFLVSDDDYFKLL 590

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BLAST of Chy8G154980 vs. ExPASy Swiss-Prot
Match: A0FKE6 (Threonine dehydratase 1 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4081 GN=TD1 PE=1 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 4.6e-106
Identity = 192/342 (56.14%), Postives = 260/342 (76.02%), Query Frame = 0


Query: 1   MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQV 60
           MEI RQ++  +HAIFVPVGGGGL+AG+A++ K V P +KIIGVEP DAN++A +LH+ Q 
Sbjct: 263 MEINRQLKDNIHAIFVPVGGGGLIAGIAAYLKRVAPDIKIIGVEPLDANALALSLHHGQR 322

Query: 61  VKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 120
           V L+ VG F DGVAVK VG+E +R+  ELIDG+VLV +DAI A IKDMFE+ RSILEPAG
Sbjct: 323 VMLDQVGGFADGVAVKVVGEETYRLCEELIDGVVLVGRDAICASIKDMFEEKRSILEPAG 382

Query: 121 ALSIAGAKAYCQHNKIKG--VKAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 180
           AL++AGA+AYC++  +KG  V A+TSGANMNFD+L  + + AD G Q EA  AT +PE P
Sbjct: 383 ALALAGAEAYCKYYGLKGENVVAITSGANMNFDRLRLVTELADVGRQREAVLATFMPEDP 442

Query: 181 GSLRTFSDLMGSRSVTEFKYRFNSEKD-AIVLYSVGVKVASELGEVKKKTESSPFETYDL 240
           GS + F++++G  ++TEFKYR+NS+K+ A+VLYSVG+    EL  + ++ ES+  +T +L
Sbjct: 443 GSFKKFAEMVGPMNITEFKYRYNSDKERALVLYSVGLHTILELEGMVERMESADLQTINL 502

Query: 241 TKNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGI 300
           T N+LVKDHLR++MGGR+++ NE+  RFT P+  GAL++FLDAFSPRWNISL HYR QG 
Sbjct: 503 TDNDLVKDHLRHLMGGRTNVHNELLCRFTFPEKPGALMKFLDAFSPRWNISLFHYRAQGD 562

Query: 301 ISADVLVGLQIEGSEEAEFHESARKLGFDYVVVANDPASKLL 340
             A+VLVG+Q+   E  EF   A  LG++Y + + + A +L+
Sbjct: 563 TGANVLVGIQVPPDEVVEFEGRADSLGYEYAMESLNEAYQLI 604

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BLAST of Chy8G154980 vs. ExPASy Swiss-Prot
Match: Q9AXU4 (Threonine dehydratase OS=Nicotiana attenuata OX=49451 GN=TD PE=1 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 2.1e-98
Identity = 183/343 (53.35%), Postives = 248/343 (72.30%), Query Frame = 0


Query: 2   EIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQVV 61
           EI RQ++  +HA+FVPVGGGGL+AGVA+++K V P  KIIGVEP  A+SM  +L++ + V
Sbjct: 260 EINRQLK-DIHAVFVPVGGGGLIAGVAAYFKRVAPHTKIIGVEPFGASSMTQSLYHGERV 319

Query: 62  KLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAGA 121
           KLE V  F DGVAV  VG+E FR+ ++LIDG+VLV  DAI+A +KD++++ R+ILE +GA
Sbjct: 320 KLEQVDNFADGVAVALVGEETFRLCKDLIDGMVLVSNDAISAAVKDVYDEGRNILETSGA 379

Query: 122 LSIAGAKAYCQHNKIKG--VKAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKPG 181
           L+IAGA+AYC++  IKG  V A+ SGANM+F +L  + D AD G Q EA  AT +PE+PG
Sbjct: 380 LAIAGAEAYCKYYNIKGENVVAIASGANMDFSKLKLVVDLADIGGQREALLATFMPEEPG 439

Query: 182 SLRTFSDLMGSRSVTEFKYRFNS-EKDAIVLYSVGVKVASELGEVKKKTESSPFETYDLT 241
           S + F +L+G  ++TEFKYR+NS  K A+VLYSVGV   S+L  + ++ +SS   T +LT
Sbjct: 440 SFKKFCELVGPMNITEFKYRYNSGRKQALVLYSVGVNTKSDLESMLERMKSSQLNTVNLT 499

Query: 242 KNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGII 301
            N LVK+HLR++MGGRS   NE+F +F  P+  GAL +FLDAFSPRWNISL HYR QG +
Sbjct: 500 NNNLVKEHLRHLMGGRSEPSNEIFCQFIFPEKPGALRKFLDAFSPRWNISLFHYREQGEL 559

Query: 302 SADVLVGLQIEGSEEAEFHESARKLGFDYVVVANDPASKLLTQ 342
            A VLVG Q+   E  EF   A  LG+ Y + + + AS+L+ +
Sbjct: 560 DASVLVGFQVPKGEIEEFRVQANNLGYSYEIESLNEASQLIME 601

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BLAST of Chy8G154980 vs. ExPASy Swiss-Prot
Match: P53607 (L-threonine dehydratase biosynthetic IlvA OS=Burkholderia multivorans (strain ATCC 17616 / 249) OX=395019 GN=ilvA PE=3 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.5e-88
Identity = 165/341 (48.39%), Postives = 232/341 (68.04%), Query Frame = 0


Query: 1   MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQV 60
           MEI RQ +GP+HAIFVP+GGGGL AGVA++ K V P++K+IGV+  D+ +MA +L   + 
Sbjct: 164 MEILRQHQGPIHAIFVPIGGGGLAAGVAAYVKAVRPEIKVIGVQAEDSCAMAQSLQAGKR 223

Query: 61  VKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 120
           V+L  VG F DG AVK VG+E FR+ +E +DG+V VD DA+ A IKD+F+DTRS+LEP+G
Sbjct: 224 VELAEVGLFADGTAVKLVGEETFRLCKEYLDGVVTVDTDALCAAIKDVFQDTRSVLEPSG 283

Query: 121 ALSIAGAKAYCQHNKIKG--VKAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 180
           AL++AGAK Y +   I+   + AVTSGANMNFD++  +A+ A+ G   EA FA  +PE+ 
Sbjct: 284 ALAVAGAKLYAEREGIENQTLVAVTSGANMNFDRMRFVAERAEVGEAREAVFAVTIPEER 343

Query: 181 GSLRTFSDLMGSRSVTEFKYRFNSEKDAIVLYSVGVKVASELGEVKKKTESSPFETYDLT 240
           GS + F  L+G R+VTEF YR    + A +   V ++   E  ++    ES  F+T DLT
Sbjct: 344 GSFKRFCSLVGDRNVTEFNYRIADAQSAHIFVGVQIRRRGESADIAANFESHGFKTADLT 403

Query: 241 KNELVKDHLRYMMGGRSSIP-NEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGI 300
            +EL K+H+RYM+GGRS +  +E  +RF  P+  GAL++FL + +P WNISL HYR QG 
Sbjct: 404 HDELSKEHIRYMVGGRSPLALDERLFRFEFPERPGALMKFLSSMAPDWNISLFHYRNQGA 463

Query: 301 ISADVLVGLQIEGSEEAEFHESARKLGFDYVVVANDPASKL 339
             + +LVGLQ+  ++ AEF      LG+ YV  + +PA +L
Sbjct: 464 DYSSILVGLQVPQADHAEFERFLAALGYPYVEESANPAYRL 504

[Close]

BLAST of Chy8G154980 vs. ExPASy Swiss-Prot
Match: P31212 (Threonine dehydratase biosynthetic, chloroplastic (Fragment) OS=Solanum tuberosum OX=4113 GN=TD PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 8.4e-84
Identity = 164/329 (49.85%), Postives = 228/329 (69.30%), Query Frame = 0


Query: 2   EIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQVV 61
           EI RQ++  +HA+FVPVGGGGL++GVA+++  V P  KIIGVEP  A SM  +L+    V
Sbjct: 17  EINRQLK-DIHAVFVPVGGGGLISGVAAYFTQVAPHTKIIGVEPYGAASMTLSLYEGHRV 76

Query: 62  KLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAGA 121
           KLE V TF DGVAV  VG+  F   +ELIDG+VLV  D I+A IKD++++ R+ILE +GA
Sbjct: 77  KLENVDTFADGVAVALVGEYTFAKCQELIDGMVLVRNDGISAAIKDVYDEGRNILETSGA 136

Query: 122 LSIAGAKAYCQHNKIK--GVKAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKPG 181
           ++IAGA AYC+   IK   + A+ SGANM+F +L  + + A+ G+  EA  AT + E+PG
Sbjct: 137 VAIAGAAAYCEFYNIKNENIVAIASGANMDFSKLHKVTELAELGSDNEALLATFMIEQPG 196

Query: 182 SLRTFSDLMGSRSVTEFKYRFNSE-KDAIVLYSVGVKVASELGEVKKKTESSPFETYDLT 241
           S +TF+ L+GS ++TE  YRF SE K+A+VLY V V   S+L E+ KK  SS  +T++ +
Sbjct: 197 SFKTFAKLVGSMNITEVTYRFTSERKEALVLYRVDVDEKSDLEEMIKKLNSSNMKTFNFS 256

Query: 242 KNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGII 301
            NELV +H+++++GG +SI +E+F  F  P+ +G L  FL+AFSPRWNI+L  YR QG I
Sbjct: 257 HNELVAEHIKHLVGGSASISDEIFGEFIFPEKAGTLSTFLEAFSPRWNITLCRYRDQGDI 316

Query: 302 SADVLVGLQIEGSEEAEFHESARKLGFDY 328
           + +VLVG Q+  SE  EF   A  LG+ Y
Sbjct: 317 NGNVLVGFQVPQSEMDEFKSQADGLGYPY 344

[Close]

BLAST of Chy8G154980 vs. ExPASy TrEMBL
Match: A0A0A0KHC3 (Threonine dehydratase OS=Cucumis sativus OX=3659 GN=Csa_6G448720 PE=3 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 1.7e-180
Identity = 329/345 (95.36%), Postives = 336/345 (97.39%), Query Frame = 0


Query: 1   MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQV 60
           MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFP+VKIIGVEPNDANSMASALHNDQV
Sbjct: 275 MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPEVKIIGVEPNDANSMASALHNDQV 334

Query: 61  VKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 120
           VKLETVGTF DGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG
Sbjct: 335 VKLETVGTFADGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 394

Query: 121 ALSIAGAKAYCQHNKIKGVK--AVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 180
           ALSIAGAKAYC++N IKGV   AVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP
Sbjct: 395 ALSIAGAKAYCEYNNIKGVNIVAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 454

Query: 181 GSLRTFSDLMGSRSVTEFKYRFNSEKDAIVLYSVGVKVASELGEVKKKTESSPFETYDLT 240
           GSL TFSDLMGSRSVTEFKYRFNSEK+AIVLYSVGVKVASELGEVKKK ESSPFETYDLT
Sbjct: 455 GSLITFSDLMGSRSVTEFKYRFNSEKNAIVLYSVGVKVASELGEVKKKIESSPFETYDLT 514

Query: 241 KNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGII 300
           KNELVKDHLRYMMGGRSS+PNEVFYRFTLP+ SGALLQFLDAFSPRWNISLIHYRRQGII
Sbjct: 515 KNELVKDHLRYMMGGRSSVPNEVFYRFTLPERSGALLQFLDAFSPRWNISLIHYRRQGII 574

Query: 301 SADVLVGLQIEGSEEAEFHESARKLGFDYVVVANDPASKLLTQVV 344
           SADVLVGLQIEGSEEAEFHESARKLGF YVVV+NDPASKLLTQVV
Sbjct: 575 SADVLVGLQIEGSEEAEFHESARKLGFGYVVVSNDPASKLLTQVV 619

[Close]

BLAST of Chy8G154980 vs. ExPASy TrEMBL
Match: A0A5A7SI54 (Threonine dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001690 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 4.4e-136
Identity = 256/346 (73.99%), Postives = 296/346 (85.55%), Query Frame = 0


Query: 1   MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQV 60
           MEIG QMR PL AIFVPVGGGGLLAGVASFYKLV+PKVKIIGVEP DANSMASAL+NDQV
Sbjct: 262 MEIGHQMREPLDAIFVPVGGGGLLAGVASFYKLVYPKVKIIGVEPYDANSMASALYNDQV 321

Query: 61  VKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 120
           V+L+TVGTF DGV +K+VG ENFRISREL+DGIVLVDK  +AA IK+++EDT+S+LEPAG
Sbjct: 322 VQLDTVGTFADGVDIKRVGGENFRISRELVDGIVLVDKHDMAATIKEVYEDTKSMLEPAG 381

Query: 121 ALSIAGAKAYCQHNKIKG--VKAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 180
           AL++AGAKAYC++N IKG  V A+TSGANMNFDQLGSIAD  +S    EATFATILPEKP
Sbjct: 382 ALAVAGAKAYCKYNNIKGGNVVAITSGANMNFDQLGSIADKVES----EATFATILPEKP 441

Query: 181 GSLR-TFSDLMGSRSVTEFKYRFNSEKDAIVLYSVGVKVASELGEVKKKTESSPFETYDL 240
           G+L+ T S L+GSR++TE KYR NSEKDAIV YSV +   SEL +VKK+ ESS FETYDL
Sbjct: 442 GTLKSTLSHLVGSRNITEIKYRHNSEKDAIVFYSVWLSDVSELEDVKKQIESSTFETYDL 501

Query: 241 TKNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGI 300
           T NE+ K+HLRYM+GGRS++PNEVFYRFTLP+  GAL Q LDA SPRWNISLIHYRRQG 
Sbjct: 502 TNNEVFKNHLRYMVGGRSNVPNEVFYRFTLPERPGALSQCLDALSPRWNISLIHYRRQGT 561

Query: 301 ISADVLVGLQIEGSEEAEFHESARKLGFDYVVVA-NDPASKLLTQV 343
           ISADVLVGLQ+  S+  EF+E A+KLGF+YV VA +DPASKL T +
Sbjct: 562 ISADVLVGLQVPDSDMGEFNERAKKLGFEYVAVAYDDPASKLFTYI 603

[Close]

BLAST of Chy8G154980 vs. ExPASy TrEMBL
Match: A0A5A7SJE6 (Threonine dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001670 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 8.3e-135
Identity = 251/346 (72.54%), Postives = 297/346 (85.84%), Query Frame = 0


Query: 1   MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQV 60
           MEIGRQMR PL AIFVPVGGGGLLAGVASFYKL++P+VKIIGVEP+DANSMASAL++DQ+
Sbjct: 262 MEIGRQMREPLDAIFVPVGGGGLLAGVASFYKLIYPEVKIIGVEPHDANSMASALYSDQI 321

Query: 61  VKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 120
           V++  +GTF DGV +K+VGDE FRISREL+DGIVLVDK+ IAA IK+++EDT+S+LEPAG
Sbjct: 322 VQVFDIGTFADGVDIKRVGDETFRISRELVDGIVLVDKNDIAAAIKEVYEDTKSMLEPAG 381

Query: 121 ALSIAGAKAYCQHNKIKG--VKAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 180
           AL++AGAKAYC++N IKG  V A+TSGANMNFDQLGSIAD  +S    EATFATILPEKP
Sbjct: 382 ALAVAGAKAYCKYNNIKGGNVVAITSGANMNFDQLGSIADKVES----EATFATILPEKP 441

Query: 181 GSLR-TFSDLMGSRSVTEFKYRFNSEKDAIVLYSVGVKVASELGEVKKKTESSPFETYDL 240
           G+L+ T S L+GSR++TE KYR NSEKDAIV YSV +   SEL +VKK+ ESS FETYDL
Sbjct: 442 GTLKSTLSHLVGSRNITEIKYRHNSEKDAIVFYSVWLSDVSELEDVKKQIESSTFETYDL 501

Query: 241 TKNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGI 300
           T NE+ K+HLRYM+GGRS++PNEVFYRFTLP+  GAL Q LDA SPRWNISLIHYRRQG 
Sbjct: 502 TNNEVFKNHLRYMVGGRSNVPNEVFYRFTLPERPGALSQCLDALSPRWNISLIHYRRQGT 561

Query: 301 ISADVLVGLQIEGSEEAEFHESARKLGFDYVVVA-NDPASKLLTQV 343
           ISADVLVGLQ+  S+  EF+E A+KLGF+YV VA +DPASKL T +
Sbjct: 562 ISADVLVGLQVPDSDMGEFNERAKKLGFEYVAVAYDDPASKLFTYI 603

[Close]

BLAST of Chy8G154980 vs. ExPASy TrEMBL
Match: A0A5D3C648 (Threonine dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00760 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.2e-133
Identity = 249/346 (71.97%), Postives = 296/346 (85.55%), Query Frame = 0


Query: 1   MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQV 60
           MEIGRQMR PL AIFVPVGGGGLLAGVASFYKL++P+VKIIGVEP+DANSMASAL++DQ+
Sbjct: 262 MEIGRQMREPLDAIFVPVGGGGLLAGVASFYKLIYPEVKIIGVEPHDANSMASALYSDQI 321

Query: 61  VKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 120
           V++  +GTF DGV +K+VGDE FRISREL+DGIVLVDK+ IAA IK+++EDT+S+LEPAG
Sbjct: 322 VQVFDIGTFADGVDIKRVGDETFRISRELVDGIVLVDKNDIAAAIKEVYEDTKSMLEPAG 381

Query: 121 ALSIAGAKAYCQHNKIKG--VKAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 180
           AL++AGAKAYC++N IKG  V A+TSGANMNFDQLGSIAD  +S    EATFATILPEKP
Sbjct: 382 ALAVAGAKAYCKYNNIKGGNVVAITSGANMNFDQLGSIADKVES----EATFATILPEKP 441

Query: 181 GSLR-TFSDLMGSRSVTEFKYRFNSEKDAIVLYSVGVKVASELGEVKKKTESSPFETYDL 240
           G+L+ T S L+GSR++TE KYR NSEKDA+V YSV +   SEL +VKK+ ESS FETYDL
Sbjct: 442 GTLKSTLSHLVGSRNITEIKYRHNSEKDAMVFYSVWLSDVSELEDVKKQIESSTFETYDL 501

Query: 241 TKNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGI 300
           T NE+ K+HLRYM+GGRS++PNEVFYRFTLP+  GAL Q LDA SPRWNISLIHYRRQG 
Sbjct: 502 TNNEVFKNHLRYMVGGRSNVPNEVFYRFTLPERPGALSQCLDALSPRWNISLIHYRRQGT 561

Query: 301 ISADVLVGLQIEGSEEAEFHESARKLGFDYVVVA-NDPASKLLTQV 343
           ISADVLVGLQ+  S+  EF+E A+KLGF+Y  VA +DPASKL T +
Sbjct: 562 ISADVLVGLQVPDSDMGEFNERAKKLGFEYGAVAYDDPASKLFTYI 603

[Close]

BLAST of Chy8G154980 vs. ExPASy TrEMBL
Match: A0A0A0KET0 (Threonine dehydratase OS=Cucumis sativus OX=3659 GN=Csa_6G448730 PE=3 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 8.1e-130
Identity = 243/346 (70.23%), Postives = 288/346 (83.24%), Query Frame = 0


Query: 1   MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQV 60
           MEIG QMR P+ AIFVPVGGGGL+AGVAS+YKLV+PKVKIIGVEP DANSMASAL+NDQV
Sbjct: 265 MEIGHQMREPVDAIFVPVGGGGLIAGVASYYKLVYPKVKIIGVEPYDANSMASALYNDQV 324

Query: 61  VKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 120
           V+++ VGTF DGV +K+VGDE FRISREL+DGIVLVDK  +AA IK+++EDT+S+LEPAG
Sbjct: 325 VQVQDVGTFADGVDIKRVGDETFRISRELVDGIVLVDKHEMAAAIKEVYEDTKSMLEPAG 384

Query: 121 ALSIAGAKAYCQHNKIKGVK--AVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 180
           AL++AGAKAY ++N IKGV   A+TSGANMNFDQL SIA   DS    EATFA+ILPEKP
Sbjct: 385 ALAVAGAKAYYKYNNIKGVNVVAITSGANMNFDQLSSIAGKVDS----EATFASILPEKP 444

Query: 181 GSLRT-FSDLMGSRSVTEFKYRFNSEKDAIVLYSVGVKVASELGEVKKKTESSPFETYDL 240
           GSL++  S L+GSR++TE KYR NSEKDAIV YSV +   SEL ++KK+ ESS FET+DL
Sbjct: 445 GSLKSALSHLVGSRNITEIKYRHNSEKDAIVFYSVWLSEISELEDMKKQIESSTFETFDL 504

Query: 241 TKNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGI 300
           T NE+ KDH RYM+GGRS++PNEVFYRFTLP   G+L Q LDA SPRW+ISLIHYRRQG 
Sbjct: 505 TNNEVFKDHFRYMVGGRSNVPNEVFYRFTLPDRPGSLSQCLDALSPRWDISLIHYRRQGT 564

Query: 301 ISADVLVGLQIEGSEEAEFHESARKLGFDYVVVA-NDPASKLLTQV 343
           IS DVL+GLQ+  SE  E +ESA+KLGFDY  V+ NDPASKL T +
Sbjct: 565 ISGDVLIGLQVRDSEMDELNESAKKLGFDYAAVSYNDPASKLFTNI 606

[Close]

BLAST of Chy8G154980 vs. NCBI nr
Match: XP_004143369.1 (threonine dehydratase biosynthetic, chloroplastic [Cucumis sativus] >KGN48214.1 hypothetical protein Csa_003609 [Cucumis sativus])

HSP 1 Score: 640 bits (1652), Expect = 1.34e-226
Identity = 329/345 (95.36%), Postives = 336/345 (97.39%), Query Frame = 0


Query: 1   MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQV 60
           MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFP+VKIIGVEPNDANSMASALHNDQV
Sbjct: 275 MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPEVKIIGVEPNDANSMASALHNDQV 334

Query: 61  VKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 120
           VKLETVGTF DGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG
Sbjct: 335 VKLETVGTFADGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 394

Query: 121 ALSIAGAKAYCQHNKIKGVK--AVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 180
           ALSIAGAKAYC++N IKGV   AVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP
Sbjct: 395 ALSIAGAKAYCEYNNIKGVNIVAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 454

Query: 181 GSLRTFSDLMGSRSVTEFKYRFNSEKDAIVLYSVGVKVASELGEVKKKTESSPFETYDLT 240
           GSL TFSDLMGSRSVTEFKYRFNSEK+AIVLYSVGVKVASELGEVKKK ESSPFETYDLT
Sbjct: 455 GSLITFSDLMGSRSVTEFKYRFNSEKNAIVLYSVGVKVASELGEVKKKIESSPFETYDLT 514

Query: 241 KNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGII 300
           KNELVKDHLRYMMGGRSS+PNEVFYRFTLP+ SGALLQFLDAFSPRWNISLIHYRRQGII
Sbjct: 515 KNELVKDHLRYMMGGRSSVPNEVFYRFTLPERSGALLQFLDAFSPRWNISLIHYRRQGII 574

Query: 301 SADVLVGLQIEGSEEAEFHESARKLGFDYVVVANDPASKLLTQVV 343
           SADVLVGLQIEGSEEAEFHESARKLGF YVVV+NDPASKLLTQVV
Sbjct: 575 SADVLVGLQIEGSEEAEFHESARKLGFGYVVVSNDPASKLLTQVV 619

[Close]

BLAST of Chy8G154980 vs. NCBI nr
Match: KAA0025314.1 (threonine dehydratase biosynthetic [Cucumis melo var. makuwa])

HSP 1 Score: 493 bits (1269), Expect = 9.99e-169
Identity = 256/346 (73.99%), Postives = 296/346 (85.55%), Query Frame = 0


Query: 1   MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQV 60
           MEIG QMR PL AIFVPVGGGGLLAGVASFYKLV+PKVKIIGVEP DANSMASAL+NDQV
Sbjct: 262 MEIGHQMREPLDAIFVPVGGGGLLAGVASFYKLVYPKVKIIGVEPYDANSMASALYNDQV 321

Query: 61  VKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 120
           V+L+TVGTF DGV +K+VG ENFRISREL+DGIVLVDK  +AA IK+++EDT+S+LEPAG
Sbjct: 322 VQLDTVGTFADGVDIKRVGGENFRISRELVDGIVLVDKHDMAATIKEVYEDTKSMLEPAG 381

Query: 121 ALSIAGAKAYCQHNKIKG--VKAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 180
           AL++AGAKAYC++N IKG  V A+TSGANMNFDQLGSIAD  +S    EATFATILPEKP
Sbjct: 382 ALAVAGAKAYCKYNNIKGGNVVAITSGANMNFDQLGSIADKVES----EATFATILPEKP 441

Query: 181 GSLR-TFSDLMGSRSVTEFKYRFNSEKDAIVLYSVGVKVASELGEVKKKTESSPFETYDL 240
           G+L+ T S L+GSR++TE KYR NSEKDAIV YSV +   SEL +VKK+ ESS FETYDL
Sbjct: 442 GTLKSTLSHLVGSRNITEIKYRHNSEKDAIVFYSVWLSDVSELEDVKKQIESSTFETYDL 501

Query: 241 TKNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGI 300
           T NE+ K+HLRYM+GGRS++PNEVFYRFTLP+  GAL Q LDA SPRWNISLIHYRRQG 
Sbjct: 502 TNNEVFKNHLRYMVGGRSNVPNEVFYRFTLPERPGALSQCLDALSPRWNISLIHYRRQGT 561

Query: 301 ISADVLVGLQIEGSEEAEFHESARKLGFDYVVVA-NDPASKLLTQV 342
           ISADVLVGLQ+  S+  EF+E A+KLGF+YV VA +DPASKL T +
Sbjct: 562 ISADVLVGLQVPDSDMGEFNERAKKLGFEYVAVAYDDPASKLFTYI 603

[Close]

BLAST of Chy8G154980 vs. NCBI nr
Match: KAA0025312.1 (threonine dehydratase biosynthetic [Cucumis melo var. makuwa])

HSP 1 Score: 489 bits (1258), Expect = 4.63e-167
Identity = 251/346 (72.54%), Postives = 297/346 (85.84%), Query Frame = 0


Query: 1   MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQV 60
           MEIGRQMR PL AIFVPVGGGGLLAGVASFYKL++P+VKIIGVEP+DANSMASAL++DQ+
Sbjct: 262 MEIGRQMREPLDAIFVPVGGGGLLAGVASFYKLIYPEVKIIGVEPHDANSMASALYSDQI 321

Query: 61  VKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 120
           V++  +GTF DGV +K+VGDE FRISREL+DGIVLVDK+ IAA IK+++EDT+S+LEPAG
Sbjct: 322 VQVFDIGTFADGVDIKRVGDETFRISRELVDGIVLVDKNDIAAAIKEVYEDTKSMLEPAG 381

Query: 121 ALSIAGAKAYCQHNKIKG--VKAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 180
           AL++AGAKAYC++N IKG  V A+TSGANMNFDQLGSIAD  +S    EATFATILPEKP
Sbjct: 382 ALAVAGAKAYCKYNNIKGGNVVAITSGANMNFDQLGSIADKVES----EATFATILPEKP 441

Query: 181 GSLR-TFSDLMGSRSVTEFKYRFNSEKDAIVLYSVGVKVASELGEVKKKTESSPFETYDL 240
           G+L+ T S L+GSR++TE KYR NSEKDAIV YSV +   SEL +VKK+ ESS FETYDL
Sbjct: 442 GTLKSTLSHLVGSRNITEIKYRHNSEKDAIVFYSVWLSDVSELEDVKKQIESSTFETYDL 501

Query: 241 TKNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGI 300
           T NE+ K+HLRYM+GGRS++PNEVFYRFTLP+  GAL Q LDA SPRWNISLIHYRRQG 
Sbjct: 502 TNNEVFKNHLRYMVGGRSNVPNEVFYRFTLPERPGALSQCLDALSPRWNISLIHYRRQGT 561

Query: 301 ISADVLVGLQIEGSEEAEFHESARKLGFDYVVVA-NDPASKLLTQV 342
           ISADVLVGLQ+  S+  EF+E A+KLGF+YV VA +DPASKL T +
Sbjct: 562 ISADVLVGLQVPDSDMGEFNERAKKLGFEYVAVAYDDPASKLFTYI 603

[Close]

BLAST of Chy8G154980 vs. NCBI nr
Match: TYK07357.1 (threonine dehydratase biosynthetic [Cucumis melo var. makuwa])

HSP 1 Score: 485 bits (1248), Expect = 1.51e-165
Identity = 249/346 (71.97%), Postives = 296/346 (85.55%), Query Frame = 0


Query: 1   MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQV 60
           MEIGRQMR PL AIFVPVGGGGLLAGVASFYKL++P+VKIIGVEP+DANSMASAL++DQ+
Sbjct: 262 MEIGRQMREPLDAIFVPVGGGGLLAGVASFYKLIYPEVKIIGVEPHDANSMASALYSDQI 321

Query: 61  VKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 120
           V++  +GTF DGV +K+VGDE FRISREL+DGIVLVDK+ IAA IK+++EDT+S+LEPAG
Sbjct: 322 VQVFDIGTFADGVDIKRVGDETFRISRELVDGIVLVDKNDIAAAIKEVYEDTKSMLEPAG 381

Query: 121 ALSIAGAKAYCQHNKIKG--VKAVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 180
           AL++AGAKAYC++N IKG  V A+TSGANMNFDQLGSIAD  +S    EATFATILPEKP
Sbjct: 382 ALAVAGAKAYCKYNNIKGGNVVAITSGANMNFDQLGSIADKVES----EATFATILPEKP 441

Query: 181 GSLR-TFSDLMGSRSVTEFKYRFNSEKDAIVLYSVGVKVASELGEVKKKTESSPFETYDL 240
           G+L+ T S L+GSR++TE KYR NSEKDA+V YSV +   SEL +VKK+ ESS FETYDL
Sbjct: 442 GTLKSTLSHLVGSRNITEIKYRHNSEKDAMVFYSVWLSDVSELEDVKKQIESSTFETYDL 501

Query: 241 TKNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGI 300
           T NE+ K+HLRYM+GGRS++PNEVFYRFTLP+  GAL Q LDA SPRWNISLIHYRRQG 
Sbjct: 502 TNNEVFKNHLRYMVGGRSNVPNEVFYRFTLPERPGALSQCLDALSPRWNISLIHYRRQGT 561

Query: 301 ISADVLVGLQIEGSEEAEFHESARKLGFDYVVVA-NDPASKLLTQV 342
           ISADVLVGLQ+  S+  EF+E A+KLGF+Y  VA +DPASKL T +
Sbjct: 562 ISADVLVGLQVPDSDMGEFNERAKKLGFEYGAVAYDDPASKLFTYI 603

[Close]

BLAST of Chy8G154980 vs. NCBI nr
Match: XP_004143289.1 (threonine dehydratase biosynthetic, chloroplastic [Cucumis sativus] >KGN48215.1 hypothetical protein Csa_003147 [Cucumis sativus])

HSP 1 Score: 472 bits (1215), Expect = 1.64e-160
Identity = 243/346 (70.23%), Postives = 288/346 (83.24%), Query Frame = 0


Query: 1   MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQV 60
           MEIG QMR P+ AIFVPVGGGGL+AGVAS+YKLV+PKVKIIGVEP DANSMASAL+NDQV
Sbjct: 265 MEIGHQMREPVDAIFVPVGGGGLIAGVASYYKLVYPKVKIIGVEPYDANSMASALYNDQV 324

Query: 61  VKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 120
           V+++ VGTF DGV +K+VGDE FRISREL+DGIVLVDK  +AA IK+++EDT+S+LEPAG
Sbjct: 325 VQVQDVGTFADGVDIKRVGDETFRISRELVDGIVLVDKHEMAAAIKEVYEDTKSMLEPAG 384

Query: 121 ALSIAGAKAYCQHNKIKGVK--AVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 180
           AL++AGAKAY ++N IKGV   A+TSGANMNFDQL SIA   DS    EATFA+ILPEKP
Sbjct: 385 ALAVAGAKAYYKYNNIKGVNVVAITSGANMNFDQLSSIAGKVDS----EATFASILPEKP 444

Query: 181 GSLRT-FSDLMGSRSVTEFKYRFNSEKDAIVLYSVGVKVASELGEVKKKTESSPFETYDL 240
           GSL++  S L+GSR++TE KYR NSEKDAIV YSV +   SEL ++KK+ ESS FET+DL
Sbjct: 445 GSLKSALSHLVGSRNITEIKYRHNSEKDAIVFYSVWLSEISELEDMKKQIESSTFETFDL 504

Query: 241 TKNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGI 300
           T NE+ KDH RYM+GGRS++PNEVFYRFTLP   G+L Q LDA SPRW+ISLIHYRRQG 
Sbjct: 505 TNNEVFKDHFRYMVGGRSNVPNEVFYRFTLPDRPGSLSQCLDALSPRWDISLIHYRRQGT 564

Query: 301 ISADVLVGLQIEGSEEAEFHESARKLGFDYVVVA-NDPASKLLTQV 342
           IS DVL+GLQ+  SE  E +ESA+KLGFDY  V+ NDPASKL T +
Sbjct: 565 ISGDVLIGLQVRDSEMDELNESAKKLGFDYAAVSYNDPASKLFTNI 606

[Close]

BLAST of Chy8G154980 vs. TAIR 10
Match: AT3G10050.1 (L-O-methylthreonine resistant 1 )

HSP 1 Score: 407.9 bits (1047), Expect = 8.0e-114
Identity = 203/341 (59.53%), Postives = 266/341 (78.01%), Query Frame = 0


Query: 1   MEIGRQMRGPLHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQV 60
           MEI RQ +GPLHAIFVPVGGGGL+AG+A++ K V P+VKIIGVEP DAN+MA +LH+ + 
Sbjct: 250 MEITRQAKGPLHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPADANAMALSLHHGER 309

Query: 61  VKLETVGTFVDGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAG 120
           V L+ VG F DGVAVK+VG+E FRISR L+DG+VLV +DAI A IKDMFE+ R+ILEPAG
Sbjct: 310 VILDQVGGFADGVAVKEVGEETFRISRNLMDGVVLVTRDAICASIKDMFEEKRNILEPAG 369

Query: 121 ALSIAGAKAYCQHNKIKGVK--AVTSGANMNFDQLGSIADNADSGNQTEATFATILPEKP 180
           AL++AGA+AYC++  +K V   A+TSGANMNFD+L  + + A+ G Q EA  AT++PEKP
Sbjct: 370 ALALAGAEAYCKYYGLKDVNVVAITSGANMNFDKLRIVTELANVGRQQEAVLATLMPEKP 429

Query: 181 GSLRTFSDLMGSRSVTEFKYRFNSEKDAIVLYSVGVKVASELGEVKKKTESSPFETYDLT 240
           GS + F +L+G  +++EFKYR +SEK+A+VLYSVGV  A EL  ++K+ ESS  +T +LT
Sbjct: 430 GSFKQFCELVGPMNISEFKYRCSSEKEAVVLYSVGVHTAGELKALQKRMESSQLKTVNLT 489

Query: 241 KNELVKDHLRYMMGGRSSIPNEVFYRFTLPKTSGALLQFLDAFSPRWNISLIHYRRQGII 300
            ++LVKDHLRY+MGGRS++ +EV  RFT P+  GAL+ FLD+FSPRWNI+L HYR QG  
Sbjct: 490 TSDLVKDHLRYLMGGRSTVGDEVLCRFTFPERPGALMNFLDSFSPRWNITLFHYRGQGET 549

Query: 301 SADVLVGLQIEGSEEAEFHESARKLGFDYVVVANDPASKLL 340
            A+VLVG+Q+   E  EF   A+ LG+DY +V++D   KLL
Sbjct: 550 GANVLVGIQVPEQEMEEFKNRAKALGYDYFLVSDDDYFKLL 590

[Close]

BLAST of Chy8G154980 vs. TAIR 10
Match: AT4G11640.1 (serine racemase )

HSP 1 Score: 76.6 bits (187), Expect = 4.2e-14
Identity = 48/157 (30.57%), Postives = 83/157 (52.87%), Query Frame = 0


Query: 11  LHAIFVPVGGGGLLAGVASFYKLVFPKVKIIGVEPNDANSMASALHNDQVVKLETVGTFV 70
           + AI VP+ GGGL++GVA   K + P ++II  EP  A+  A +    +++ L    T  
Sbjct: 177 IDAIVVPISGGGLISGVALAAKSIKPSIRIIAAEPKGADDAAQSKVAGKIITLPVTNTIA 236

Query: 71  DGVAVKQVGDENFRISRELIDGIVLVDKDAIAACIKDMFEDTRSILEPAGALSIAGAKAY 130
           DG+    +GD  + + R+L+D +V +++  I   +K  +E  +  +EP+GA+ +A   + 
Sbjct: 237 DGLRA-SLGDLTWPVVRDLVDDVVTLEECEIIEAMKMCYEILKVSVEPSGAIGLAAVLSN 296

Query: 131 CQHNK-----IKGVKAVTSGANMNFDQLGSIADNADS 163
              N       K +  V SG N++   LGS+ D+  S
Sbjct: 297 SFRNNPSCRDCKNIGIVLSGGNVD---LGSLWDSFKS 329

The following BLAST results are available for this feature:

BLAST of Chy8G154980 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZSS6	1.1e-112	59.53	Threonine dehydratase biosynthetic, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
A0FKE6	4.6e-106	56.14	Threonine dehydratase 1 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4...	[more]
Q9AXU4	2.1e-98	53.35	Threonine dehydratase OS=Nicotiana attenuata OX=49451 GN=TD PE=1 SV=1	[more]
P53607	1.5e-88	48.39	L-threonine dehydratase biosynthetic IlvA OS=Burkholderia multivorans (strain AT...	[more]
P31212	8.4e-84	49.85	Threonine dehydratase biosynthetic, chloroplastic (Fragment) OS=Solanum tuberosu...	[more]

BLAST of Chy8G154980 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KHC3	1.7e-180	95.36	Threonine dehydratase OS=Cucumis sativus OX=3659 GN=Csa_6G448720 PE=3 SV=1	[more]
A0A5A7SI54	4.4e-136	73.99	Threonine dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold54...	[more]
A0A5A7SJE6	8.3e-135	72.54	Threonine dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold54...	[more]
A0A5D3C648	1.2e-133	71.97	Threonine dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold20...	[more]
A0A0A0KET0	8.1e-130	70.23	Threonine dehydratase OS=Cucumis sativus OX=3659 GN=Csa_6G448730 PE=3 SV=1	[more]

BLAST of Chy8G154980 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143369.1	1.34e-226	95.36	threonine dehydratase biosynthetic, chloroplastic [Cucumis sativus] >KGN48214.1 ...	[more]
KAA0025314.1	9.99e-169	73.99	threonine dehydratase biosynthetic [Cucumis melo var. makuwa]	[more]
KAA0025312.1	4.63e-167	72.54	threonine dehydratase biosynthetic [Cucumis melo var. makuwa]	[more]
TYK07357.1	1.51e-165	71.97	threonine dehydratase biosynthetic [Cucumis melo var. makuwa]	[more]
XP_004143289.1	1.64e-160	70.23	threonine dehydratase biosynthetic, chloroplastic [Cucumis sativus] >KGN48215.1 ...	[more]

BLAST of Chy8G154980 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10050.1	8.0e-114	59.53	L-O-methylthreonine resistant 1	[more]
AT4G11640.1	4.2e-14	30.57	serine racemase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001721	Threonine dehydratase, ACT-like domain	PFAM	PF00585	Thr_dehydrat_C	coord: 254..336 e-value: 9.5E-18 score: 63.8 coord: 161..247 e-value: 2.5E-20 score: 72.1
IPR001721	Threonine dehydratase, ACT-like domain	PROSITE	PS51672	ACT_LIKE	coord: 168..239 score: 13.713627
IPR001926	Pyridoxal-phosphate dependent enzyme	PFAM	PF00291	PALP	coord: 2..135 e-value: 2.6E-27 score: 96.0
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 1..166 e-value: 1.1E-41 score: 144.6
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	SUPERFAMILY	53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 1..154
IPR038110	Threonine dehydratase, ACT-like domain superfamily	GENE3D	3.40.1020.10	Biosynthetic Threonine Deaminase; Domain 3	coord: 167..340 e-value: 3.7E-53 score: 181.7
None	No IPR available	PANTHER	PTHR48078:SF1	THREONINE DEHYDRATASE	coord: 1..341
None	No IPR available	PANTHER	PTHR48078	THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED	coord: 1..341
None	No IPR available	SUPERFAMILY	55021	ACT-like	coord: 248..338
None	No IPR available	SUPERFAMILY	55021	ACT-like	coord: 166..251

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy8G154980.1	Chy8G154980.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009097	isoleucine biosynthetic process
biological_process	GO:0006567	threonine catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004794	L-threonine ammonia-lyase activity
molecular_function	GO:0030170	pyridoxal phosphate binding