Chy8G154840 (gene) Cucumber (hystrix) v1

Overview
NameChy8G154840
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionE3 ubiquitin-protein ligase RZFP34-like
LocationchrH08: 16182841 .. 16184914 (-)
RNA-Seq ExpressionChy8G154840
SyntenyChy8G154840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGATGGAGTGCGAAATGGAGGCTTGGATTTCCCCAAATGGCGACATGTCATCGATCGATCTCGGATATGGCAGCTTCGGGTAGTTCATCATTCTTAAACAGTGTCTAAATTGATTTCGATATGACTATTAGGGTTATTTTCGTTGAGGTTTTGCCTGGGAGATTTCTTATTTATTTGGTTTATAATTTGAAGGTGTTCTCATTATAGAAGGAGGTGTAAGATTAGAGCTCCTTGTTGCAATGAAATATTTGATTGCAGACATTGTCACAATGAAGCTAAGGTTGGCTTCTGATTGATTGTTATTTTCGTATGTGTGTATATATATATATATTGAGAAATTGGATTCATGAAGCTTCCCATTGATGCTCTTTATGCAGAATTCATTAGATGTGGATCCTCTCTTACGCCACGACATTCCTCGCCAGGATATGGAAAAAGTAAGTTTCCTTTCAAGTATCAATTAAGCTTCATGTTCCACTTAGGTTCTGAATTCATGTTTTATCCTTGGTTTTTTTATTCAGATCAGTTTCAAGTTTAATTTTGCTAGGATCTTAAAAACGTTTAGGAGGAGATTGCATAACAAAGAAATAGAGAGAGTATTTAGAAAGACCTTTAAATTTTCTATAAATATAAATTCTAATTCATGAGTTCTTGTTTTCTATCTCTCTACAGGTCATCTGTTCCTTGTGTGGCACAGAACAAGATGTAAGTTCTTTCCTATAAAGCCTCCTTCACCAAAAAATATGGGGAAGACAACTAACTATCTTTTCTTACTTCTTTTAGGTGCGACAATATTGTATTCAATGTGGAGTTTGTATGGGGAAGTACTTTTGCGCAAAGTGCAAGTTCTTTGATGACGATGTAGGCTTCTATCAACATCATCTAATTAACTTCGATCTATATTATTAGTTTCCTGATCTTAAATTTTTTGTTGACTTGAAACTGTTTTGCTAGGTTTCAAAGAAGCAGTACCATTGTAATGAATGTGGTATTTGCAGGTCCAAACCATTTCCTTGAACTTCTTTGTATATTATTTATCTTCCTTTCTCCGTGGCTTTCGCAATTTATTAGATACAGTTTTATTCCCTAAAGATTTCTTTCTTTCTCGCTTTGCAGAGTCGGAGGTGAGGACAACTTTTTCCACTGCAAAAAGTGTGGTAAGAACTTCGGAACAATTTTTATGATTCAACTTCTTAGAGCTCTCAACTTTTCCAGCCACTCAATTCGGAAGTTTCTTCATCTTTTTAGGTTGTTGCTATTCGAAACTGATGGAGAACTCCCACAATTGTGTGGAAAAGGCAATGCACCACAATTGTCCTGTTTGCTTTGAGGTGAAATTTCAAAACATGAAATTATTTGGTGATGTTTATATATGTCATTTATTAATATGCAATGTTCTATGGCAATAACATAATCAACCATCCGTTCCTGCAGTTTCTTTTCGATACAATGAGCGACATTTCCGTTTTGCCATGTGGACACACTATTCATTTAGAATGTGTGAAGGAGATGGAGCAACGTTTACAGTTAGGTCATCTCTTCTTTTTGCTACGTAAAATCCTTTTTGTTCTTTACGATCATTTAAATGAATTACCAAAACCATTGCTTTGTATCTGCAGATACACATGCCCTGTTTGCTCAAAATCCTATTGCGATATGTCGAGCGTATGGGAGAAACTCGATCATGAGGTTCGAACCCTAATAAACTAACCAAAGTAATAACAAATGACAACATTCACATGATCCTAACATATGTTCACTTAAAATCTCAGGTTGCATCAACGCCGATGCCACAAATGTACCAACATAAAATGGTAAGTACTGGGTGTAAACTGTAAAACAACGTGTGGTTTCTCAAAAGAAACTGCTGATTTTTTTTTTATGAACTGAAAATTCTTCTTCTTGTATTATGATCAGGTTTGGATTCTTTGCAATGACTGCAGTGAGATATCAGAGGTTAATTATCACATTGTGGCACACAAGTGCCTCAAATGCAAGTCTTATAATACAAGGCTGACCCAAGGAGGGCCTTCTTCATGTTCACCAAGGATTGCTCAAATTGTGAGATAA

mRNA sequence

ATGATGATGGAGTGCGAAATGGAGGCTTGGATTTCCCCAAATGGCGACATGTCATCGATCGATCTCGGATATGGCAGCTTCGGGTGTTCTCATTATAGAAGGAGGTGTAAGATTAGAGCTCCTTGTTGCAATGAAATATTTGATTGCAGACATTGTCACAATGAAGCTAAGAATTCATTAGATGTGGATCCTCTCTTACGCCACGACATTCCTCGCCAGGATATGGAAAAAGTCATCTGTTCCTTGTGTGGCACAGAACAAGATGTGCGACAATATTGTATTCAATGTGGAGTTTGTATGGGGAAGTACTTTTGCGCAAAGTGCAAGTTCTTTGATGACGATGTTTCAAAGAAGCAGTACCATTGTAATGAATGTGGTATTTGCAGAGTCGGAGGTGAGGACAACTTTTTCCACTGCAAAAAGTGTGGTTGTTGCTATTCGAAACTGATGGAGAACTCCCACAATTGTGTGGAAAAGGCAATGCACCACAATTGTCCTGTTTGCTTTGAGTTTCTTTTCGATACAATGAGCGACATTTCCGTTTTGCCATGTGGACACACTATTCATTTAGAATGTGTGAAGGAGATGGAGCAACGTTTACAGTTAGGTCATCTCTTCTTTTTGCTACGTAAAATCCTTTTTGTTCTTTACGATCATTTAAATGAATTACCAAAACCATTGCTTTGTATCTGCAGATACACATGCCCTGTTTGCTCAAAATCCTATTGCGATATGTCGAGCGTATGGGAGAAACTCGATCATGAGGTTGCATCAACGCCGATGCCACAAATGTACCAACATAAAATGGTTTGGATTCTTTGCAATGACTGCAGTGAGATATCAGAGGTTAATTATCACATTGTGGCACACAAGTGCCTCAAATGCAAGTCTTATAATACAAGGCTGACCCAAGGAGGGCCTTCTTCATGTTCACCAAGGATTGCTCAAATTGTGAGATAA

Coding sequence (CDS)

ATGATGATGGAGTGCGAAATGGAGGCTTGGATTTCCCCAAATGGCGACATGTCATCGATCGATCTCGGATATGGCAGCTTCGGGTGTTCTCATTATAGAAGGAGGTGTAAGATTAGAGCTCCTTGTTGCAATGAAATATTTGATTGCAGACATTGTCACAATGAAGCTAAGAATTCATTAGATGTGGATCCTCTCTTACGCCACGACATTCCTCGCCAGGATATGGAAAAAGTCATCTGTTCCTTGTGTGGCACAGAACAAGATGTGCGACAATATTGTATTCAATGTGGAGTTTGTATGGGGAAGTACTTTTGCGCAAAGTGCAAGTTCTTTGATGACGATGTTTCAAAGAAGCAGTACCATTGTAATGAATGTGGTATTTGCAGAGTCGGAGGTGAGGACAACTTTTTCCACTGCAAAAAGTGTGGTTGTTGCTATTCGAAACTGATGGAGAACTCCCACAATTGTGTGGAAAAGGCAATGCACCACAATTGTCCTGTTTGCTTTGAGTTTCTTTTCGATACAATGAGCGACATTTCCGTTTTGCCATGTGGACACACTATTCATTTAGAATGTGTGAAGGAGATGGAGCAACGTTTACAGTTAGGTCATCTCTTCTTTTTGCTACGTAAAATCCTTTTTGTTCTTTACGATCATTTAAATGAATTACCAAAACCATTGCTTTGTATCTGCAGATACACATGCCCTGTTTGCTCAAAATCCTATTGCGATATGTCGAGCGTATGGGAGAAACTCGATCATGAGGTTGCATCAACGCCGATGCCACAAATGTACCAACATAAAATGGTTTGGATTCTTTGCAATGACTGCAGTGAGATATCAGAGGTTAATTATCACATTGTGGCACACAAGTGCCTCAAATGCAAGTCTTATAATACAAGGCTGACCCAAGGAGGGCCTTCTTCATGTTCACCAAGGATTGCTCAAATTGTGAGATAA

Protein sequence

MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISVLPCGHTIHLECVKEMEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTRLTQGGPSSCSPRIAQIVR*
Homology
BLAST of Chy8G154840 vs. ExPASy Swiss-Prot
Match: Q9FFB6 (E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=1 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.2e-118
Identity = 188/301 (62.46%), Postives = 228/301 (75.75%), Query Frame = 0

Query: 20  IDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRQDMEKVI 79
           +++G G +GCSHYRRRCKIRAPCC+EIFDCRHCHNEAK+SL ++   RH++PR ++ KVI
Sbjct: 18  MEIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRHELPRHEVSKVI 77

Query: 80  CSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEDNFFHC 139
           CSLC TEQDV+Q C  CGVCMGKYFC+KCKFFDDD+SKKQYHC+ECGICR GGE+NFFHC
Sbjct: 78  CSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGICRTGGEENFFHC 137

Query: 140 KKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISVLPCGHTIHLECVKEMEQR 199
           K+C CCYSK+ME+ H CVE AMHHNCPVCFE+LFD+  DI+VL CGHT+HLEC K+M   
Sbjct: 138 KRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTMHLECTKDMG-- 197

Query: 200 LQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSKSYCDMSSVWEKLDHEVAST 259
                           L++             RYTCPVCSKS CDMS++W+KLD EVA+ 
Sbjct: 198 ----------------LHN-------------RYTCPVCSKSICDMSNLWKKLDEEVAAY 257

Query: 260 PMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTRLTQGGPS--SCSPRIAQI 319
           PMP+MY++KMVWILCNDC   + V +H++AHKC  C SYNTR TQ G    SCS  + Q+
Sbjct: 258 PMPKMYENKMVWILCNDCGSNTNVRFHLIAHKCSSCGSYNTRQTQRGSDSHSCSSGMPQV 287

BLAST of Chy8G154840 vs. ExPASy Swiss-Prot
Match: Q5JL96 (Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=39947 GN=RZFP34 PE=2 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 8.3e-110
Identity = 168/286 (58.74%), Postives = 213/286 (74.48%), Query Frame = 0

Query: 16  DMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRQDM 75
           D   ++ G   +GC+HYRRRC+IRAPCCNEIFDCRHCHNE KNS+ +D + RH++PR ++
Sbjct: 48  DYEKLEEGIMQYGCAHYRRRCRIRAPCCNEIFDCRHCHNETKNSIKIDAVKRHELPRHEV 107

Query: 76  EKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEDN 135
           ++VICSLCGTEQ+VRQ CI CGVCMGKYFC  CK FDDDVSK+QYHCN CGICR+GG++N
Sbjct: 108 QQVICSLCGTEQEVRQVCISCGVCMGKYFCEVCKLFDDDVSKQQYHCNGCGICRIGGKEN 167

Query: 136 FFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISVLPCGHTIHLECVKE 195
           FFHC KCGCCYS +++NSH CVE AMHH+CP+CFE+LF++ +D+SVLPCGHTIH++C++E
Sbjct: 168 FFHCSKCGCCYSIVLKNSHACVEGAMHHDCPICFEYLFESTNDVSVLPCGHTIHVKCLRE 227

Query: 196 MEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSKSYCDMSSVWEKLDHE 255
           ME+                                C++ CP+CSKS CDMS  WE+LD E
Sbjct: 228 MEEH-------------------------------CQFACPLCSKSVCDMSKAWERLDEE 287

Query: 256 VASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTR 302
           +A+  +     +KMV ILCNDC   SEV +H++AHKC KCKSYNTR
Sbjct: 288 LAT--ISDTCDNKMVRILCNDCGATSEVQFHLIAHKCQKCKSYNTR 300

BLAST of Chy8G154840 vs. ExPASy Swiss-Prot
Match: Q8VZK0 (E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 2.7e-92
Identity = 148/295 (50.17%), Postives = 200/295 (67.80%), Query Frame = 0

Query: 7   MEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLL 66
           MEA  SPN     +  G   FGC HY+RRC+IRAPCCNE+FDCRHCHNE+ ++L  +   
Sbjct: 1   MEA--SPN---DRLHFGKMGFGCKHYKRRCQIRAPCCNEVFDCRHCHNESTSTLR-NIYD 60

Query: 67  RHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECG 126
           RHD+ RQD+++VICS+C TEQ   Q C  CGV MG+YFC+ C F+DDD  K+Q+HC++CG
Sbjct: 61  RHDLVRQDVKQVICSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCG 120

Query: 127 ICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISVLPCGH 186
           ICRVGG +NFFHCKKCG CY+  + N+H CVE +M H+CP+C+E+LFD++ D +V+ CGH
Sbjct: 121 ICRVGGRENFFHCKKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGH 180

Query: 187 TIHLECVKEMEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSKSYCDMS 246
           T+H+EC  EM +R                                ++ CP+CS+S  DMS
Sbjct: 181 TMHVECYNEMIKR-------------------------------DKFCCPICSRSVIDMS 240

Query: 247 SVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTR 302
             W++LD E+ +T MP  Y+ K VWILCNDC++ +EV++HI+  KC  C+SYNTR
Sbjct: 241 KTWQRLDEEIEATAMPSDYRDKKVWILCNDCNDTTEVHFHIIGQKCGHCRSYNTR 258

BLAST of Chy8G154840 vs. ExPASy Swiss-Prot
Match: Q10LI1 (E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SRFP1 PE=1 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.4e-77
Identity = 130/285 (45.61%), Postives = 173/285 (60.70%), Query Frame = 0

Query: 16  DMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRQDM 75
           D S    G   FGC HYRRRC+IRAPCCN++F CRHCHNE+           H++ R  +
Sbjct: 5   DASRHGFGRMGFGCKHYRRRCRIRAPCCNDVFHCRHCHNESTKD-------GHELDRHAV 64

Query: 76  EKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEDN 135
           E VIC +C TEQ V Q C  CGVCMG+YFC+ CKFFDDDV ++ +HC +CGICRVGG+DN
Sbjct: 65  ESVICLVCDTEQPVAQVCYNCGVCMGEYFCSACKFFDDDVDREHFHCQDCGICRVGGKDN 124

Query: 136 FFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISVLPCGHTIHLECVKE 195
           FFHC+KCG CYS  + + H C+E +M +NCP+C+E+LFD++ + SVL CGHT+HL+C  E
Sbjct: 125 FFHCEKCGSCYSVSLRDKHCCIENSMKNNCPICYEYLFDSLRETSVLRCGHTMHLQCFHE 184

Query: 196 MEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSKSYCDMSSVWEKLDHE 255
           M     L H                           +++CP+CS    DM      LD E
Sbjct: 185 M-----LKH--------------------------DKFSCPICSMPIFDMDKFLRALDAE 244

Query: 256 VASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 301
           + +  +   Y  K  WI+CNDC + ++V   +  HKC  C+S+NT
Sbjct: 245 IEANMLHIDYMGK-GWIVCNDCRDTTQVYARVAGHKCCHCQSHNT 250

BLAST of Chy8G154840 vs. ExPASy Swiss-Prot
Match: Q96PM5 (RING finger and CHY zinc finger domain-containing protein 1 OS=Homo sapiens OX=9606 GN=RCHY1 PE=1 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 3.7e-57
Identity = 108/281 (38.43%), Postives = 147/281 (52.31%), Query Frame = 0

Query: 25  GSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRQDMEKVICSLCG 84
           G  GC HY R C ++APCC++++ CR CH+  ++         H + R  +++V C  C 
Sbjct: 16  GQRGCEHYDRGCLLKAPCCDKLYTCRLCHDNNED---------HQLDRFKVKEVQCINCE 75

Query: 85  TEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEDNFFHCKKCGC 144
             Q  +Q C +C    G+Y+C  C  FD D  KKQYHC  CGICR+G +++FFHC KC  
Sbjct: 76  KIQHAQQTCEECSTLFGEYYCDICHLFDKD--KKQYHCENCGICRIGPKEDFFHCLKCNL 135

Query: 145 CYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISVLPCGHTIHLECVKEMEQRLQLGH 204
           C +  ++  H C+E     NCP+C E +  +     VLPCGH +H  C +EM   L+ G 
Sbjct: 136 CLAMNLQGRHKCIENVSRQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEM---LKEG- 195

Query: 205 LFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQM 264
                                       Y CP+C  S  DM+  W +LD EVA TPMP  
Sbjct: 196 ----------------------------YRCPLCMHSALDMTRYWRQLDDEVAQTPMPSE 253

Query: 265 YQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTRLTQG 306
           YQ+  V ILCNDC+  S V +HI+  KC  C+SYNT    G
Sbjct: 256 YQNMTVDILCNDCNGRSTVQFHILGMKCKICESYNTAQAGG 253

BLAST of Chy8G154840 vs. ExPASy TrEMBL
Match: A0A0A0KIG3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G448100 PE=4 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.4e-176
Identity = 288/319 (90.28%), Postives = 288/319 (90.28%), Query Frame = 0

Query: 1   MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL 60
           MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL
Sbjct: 1   MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL 60

Query: 61  DVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQY 120
           DVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQY
Sbjct: 61  DVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQY 120

Query: 121 HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS 180
           HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS
Sbjct: 121 HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS 180

Query: 181 VLPCGHTIHLECVKEMEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSK 240
           VLPCGHTIHLECVKEMEQRLQ                               YTCPVCSK
Sbjct: 181 VLPCGHTIHLECVKEMEQRLQ-------------------------------YTCPVCSK 240

Query: 241 SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 300
           SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT
Sbjct: 241 SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 288

Query: 301 RLTQGGPSSCSPRIAQIVR 320
           RLTQGGPSSCSPRIAQIVR
Sbjct: 301 RLTQGGPSSCSPRIAQIVR 288

BLAST of Chy8G154840 vs. ExPASy TrEMBL
Match: A0A5A7V9A0 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold827G00080 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 2.9e-174
Identity = 285/318 (89.62%), Postives = 285/318 (89.62%), Query Frame = 0

Query: 2   MMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 61
           MMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD
Sbjct: 1   MMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60

Query: 62  VDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 121
           VDPLLRHDIPR DMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYH
Sbjct: 61  VDPLLRHDIPRHDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120

Query: 122 CNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISV 181
           CNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISV
Sbjct: 121 CNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISV 180

Query: 182 LPCGHTIHLECVKEMEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSKS 241
           LPCGHTIHLECVKEMEQRLQ                               YTCPVCSKS
Sbjct: 181 LPCGHTIHLECVKEMEQRLQ-------------------------------YTCPVCSKS 240

Query: 242 YCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTR 301
           YCDMSSVWEKLDHEVASTPMPQMYQ KMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTR
Sbjct: 241 YCDMSSVWEKLDHEVASTPMPQMYQDKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTR 287

Query: 302 LTQGGPSSCSPRIAQIVR 320
           LTQGGPSSCSPRIAQIVR
Sbjct: 301 LTQGGPSSCSPRIAQIVR 287

BLAST of Chy8G154840 vs. ExPASy TrEMBL
Match: A0A1S3CHL2 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo OX=3656 GN=LOC103500836 PE=4 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 5.0e-174
Identity = 285/319 (89.34%), Postives = 285/319 (89.34%), Query Frame = 0

Query: 1   MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL 60
           MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL
Sbjct: 1   MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL 60

Query: 61  DVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQY 120
            VDPLLRHDIPR DMEKVICSLCGTEQDVRQYCIQC VCMGKYFCAKCKFFDDDVSKKQY
Sbjct: 61  GVDPLLRHDIPRHDMEKVICSLCGTEQDVRQYCIQCEVCMGKYFCAKCKFFDDDVSKKQY 120

Query: 121 HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS 180
           HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS
Sbjct: 121 HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS 180

Query: 181 VLPCGHTIHLECVKEMEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSK 240
           VLPCGHTIHLECVKEMEQRLQ                               YTCPVCSK
Sbjct: 181 VLPCGHTIHLECVKEMEQRLQ-------------------------------YTCPVCSK 240

Query: 241 SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 300
           SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT
Sbjct: 241 SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 288

Query: 301 RLTQGGPSSCSPRIAQIVR 320
           RLTQGGPSSCSPRIAQIVR
Sbjct: 301 RLTQGGPSSCSPRIAQIVR 288

BLAST of Chy8G154840 vs. ExPASy TrEMBL
Match: A0A6J1I9L5 (E3 ubiquitin-protein ligase RZFP34-like OS=Cucurbita maxima OX=3661 GN=LOC111471282 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.6e-164
Identity = 270/319 (84.64%), Postives = 278/319 (87.15%), Query Frame = 0

Query: 1   MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL 60
           MM+E  MEAWIS NG+ S IDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL
Sbjct: 1   MMVESGMEAWISTNGEKSLIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL 60

Query: 61  DVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQY 120
           DVDP LRHDIPR D+EKVICSLC TEQDV+Q CIQCGVCMGKYFC+KCKFFDDDVSKKQY
Sbjct: 61  DVDPRLRHDIPRHDIEKVICSLCDTEQDVQQRCIQCGVCMGKYFCSKCKFFDDDVSKKQY 120

Query: 121 HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS 180
           HCNECGICRVGGE+NFFHCKKCGCCYSKLMENSH+CVEKAMHHNCPVCFEFLFDTMSDIS
Sbjct: 121 HCNECGICRVGGEENFFHCKKCGCCYSKLMENSHSCVEKAMHHNCPVCFEFLFDTMSDIS 180

Query: 181 VLPCGHTIHLECVKEMEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSK 240
           VLPCGHTIHLECVKEMEQRLQ                               YTCPVCSK
Sbjct: 181 VLPCGHTIHLECVKEMEQRLQ-------------------------------YTCPVCSK 240

Query: 241 SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 300
           SYCDMSSVWEKLDHEVASTPMPQMY HKMVWILCNDCSEISEVNYHIVAHKC+KCKSYNT
Sbjct: 241 SYCDMSSVWEKLDHEVASTPMPQMYLHKMVWILCNDCSEISEVNYHIVAHKCVKCKSYNT 288

Query: 301 RLTQGGPSSCSPRIAQIVR 320
           RLTQGGPSSCSPRIAQIVR
Sbjct: 301 RLTQGGPSSCSPRIAQIVR 288

BLAST of Chy8G154840 vs. ExPASy TrEMBL
Match: A0A6J1HG84 (E3 ubiquitin-protein ligase RZFP34-like OS=Cucurbita moschata OX=3662 GN=LOC111463216 PE=4 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.1e-164
Identity = 268/319 (84.01%), Postives = 280/319 (87.77%), Query Frame = 0

Query: 1   MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL 60
           M++E  MEAWIS NG+ S IDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL
Sbjct: 1   MIVESGMEAWISTNGEKSLIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL 60

Query: 61  DVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQY 120
           DVDP LRHDIPR D+EKVICSLC TEQDV+Q+CIQCGVCMGKYFC+KCKFFDDDVSKKQY
Sbjct: 61  DVDPRLRHDIPRHDIEKVICSLCDTEQDVQQHCIQCGVCMGKYFCSKCKFFDDDVSKKQY 120

Query: 121 HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS 180
           HCNECGICRVGGE+NFFHCKKCGCCYSKLMENSH+CVEKAMHHNCPVCFEFLFDT+SDIS
Sbjct: 121 HCNECGICRVGGEENFFHCKKCGCCYSKLMENSHSCVEKAMHHNCPVCFEFLFDTISDIS 180

Query: 181 VLPCGHTIHLECVKEMEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSK 240
           VLPCGHTIHLECVKEMEQRLQ                               YTCPVCSK
Sbjct: 181 VLPCGHTIHLECVKEMEQRLQ-------------------------------YTCPVCSK 240

Query: 241 SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 300
           SYCDMSSVWEKLDHEVASTP+PQMYQHKMVWILCNDCSEISEVNYHIVAHKC+KCKSYNT
Sbjct: 241 SYCDMSSVWEKLDHEVASTPIPQMYQHKMVWILCNDCSEISEVNYHIVAHKCVKCKSYNT 288

Query: 301 RLTQGGPSSCSPRIAQIVR 320
           RLTQGGPSSCSPRIAQIVR
Sbjct: 301 RLTQGGPSSCSPRIAQIVR 288

BLAST of Chy8G154840 vs. NCBI nr
Match: XP_004143281.1 (E3 ubiquitin-protein ligase RZFP34 isoform X1 [Cucumis sativus] >XP_011657675.1 E3 ubiquitin-protein ligase RZFP34 isoform X1 [Cucumis sativus] >KGN48202.1 hypothetical protein Csa_003793 [Cucumis sativus])

HSP 1 Score: 620 bits (1598), Expect = 5.21e-224
Identity = 288/319 (90.28%), Postives = 288/319 (90.28%), Query Frame = 0

Query: 1   MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL 60
           MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL
Sbjct: 1   MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL 60

Query: 61  DVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQY 120
           DVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQY
Sbjct: 61  DVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQY 120

Query: 121 HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS 180
           HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS
Sbjct: 121 HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS 180

Query: 181 VLPCGHTIHLECVKEMEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSK 240
           VLPCGHTIHLECVKEMEQRLQ                               YTCPVCSK
Sbjct: 181 VLPCGHTIHLECVKEMEQRLQ-------------------------------YTCPVCSK 240

Query: 241 SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 300
           SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT
Sbjct: 241 SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 288

Query: 301 RLTQGGPSSCSPRIAQIVR 319
           RLTQGGPSSCSPRIAQIVR
Sbjct: 301 RLTQGGPSSCSPRIAQIVR 288

BLAST of Chy8G154840 vs. NCBI nr
Match: KAA0063794.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo var. makuwa])

HSP 1 Score: 613 bits (1580), Expect = 2.79e-221
Identity = 285/318 (89.62%), Postives = 285/318 (89.62%), Query Frame = 0

Query: 2   MMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 61
           MMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD
Sbjct: 1   MMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLD 60

Query: 62  VDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 121
           VDPLLRHDIPR DMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYH
Sbjct: 61  VDPLLRHDIPRHDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYH 120

Query: 122 CNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISV 181
           CNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISV
Sbjct: 121 CNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISV 180

Query: 182 LPCGHTIHLECVKEMEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSKS 241
           LPCGHTIHLECVKEMEQRLQ                               YTCPVCSKS
Sbjct: 181 LPCGHTIHLECVKEMEQRLQ-------------------------------YTCPVCSKS 240

Query: 242 YCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTR 301
           YCDMSSVWEKLDHEVASTPMPQMYQ KMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTR
Sbjct: 241 YCDMSSVWEKLDHEVASTPMPQMYQDKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTR 287

Query: 302 LTQGGPSSCSPRIAQIVR 319
           LTQGGPSSCSPRIAQIVR
Sbjct: 301 LTQGGPSSCSPRIAQIVR 287

BLAST of Chy8G154840 vs. NCBI nr
Match: XP_008462499.1 (PREDICTED: E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo] >XP_008462500.1 PREDICTED: E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo])

HSP 1 Score: 612 bits (1578), Expect = 5.84e-221
Identity = 285/319 (89.34%), Postives = 285/319 (89.34%), Query Frame = 0

Query: 1   MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL 60
           MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL
Sbjct: 1   MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL 60

Query: 61  DVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQY 120
            VDPLLRHDIPR DMEKVICSLCGTEQDVRQYCIQC VCMGKYFCAKCKFFDDDVSKKQY
Sbjct: 61  GVDPLLRHDIPRHDMEKVICSLCGTEQDVRQYCIQCEVCMGKYFCAKCKFFDDDVSKKQY 120

Query: 121 HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS 180
           HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS
Sbjct: 121 HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS 180

Query: 181 VLPCGHTIHLECVKEMEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSK 240
           VLPCGHTIHLECVKEMEQRLQ                               YTCPVCSK
Sbjct: 181 VLPCGHTIHLECVKEMEQRLQ-------------------------------YTCPVCSK 240

Query: 241 SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 300
           SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT
Sbjct: 241 SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 288

Query: 301 RLTQGGPSSCSPRIAQIVR 319
           RLTQGGPSSCSPRIAQIVR
Sbjct: 301 RLTQGGPSSCSPRIAQIVR 288

BLAST of Chy8G154840 vs. NCBI nr
Match: XP_031743612.1 (E3 ubiquitin-protein ligase RZFP34 isoform X2 [Cucumis sativus] >XP_031743613.1 E3 ubiquitin-protein ligase RZFP34 isoform X2 [Cucumis sativus])

HSP 1 Score: 601 bits (1550), Expect = 9.00e-217
Identity = 283/319 (88.71%), Postives = 283/319 (88.71%), Query Frame = 0

Query: 1   MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL 60
           MMMECEMEAWISPNGDMSSIDLGYGSFG     RRCKIRAPCCNEIFDCRHCHNEAKNSL
Sbjct: 1   MMMECEMEAWISPNGDMSSIDLGYGSFG-----RRCKIRAPCCNEIFDCRHCHNEAKNSL 60

Query: 61  DVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQY 120
           DVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQY
Sbjct: 61  DVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQY 120

Query: 121 HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS 180
           HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS
Sbjct: 121 HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS 180

Query: 181 VLPCGHTIHLECVKEMEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSK 240
           VLPCGHTIHLECVKEMEQRLQ                               YTCPVCSK
Sbjct: 181 VLPCGHTIHLECVKEMEQRLQ-------------------------------YTCPVCSK 240

Query: 241 SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 300
           SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT
Sbjct: 241 SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 283

Query: 301 RLTQGGPSSCSPRIAQIVR 319
           RLTQGGPSSCSPRIAQIVR
Sbjct: 301 RLTQGGPSSCSPRIAQIVR 283

BLAST of Chy8G154840 vs. NCBI nr
Match: XP_038882721.1 (E3 ubiquitin-protein ligase RZFP34-like [Benincasa hispida] >XP_038882722.1 E3 ubiquitin-protein ligase RZFP34-like [Benincasa hispida])

HSP 1 Score: 595 bits (1534), Expect = 2.98e-214
Identity = 277/319 (86.83%), Postives = 280/319 (87.77%), Query Frame = 0

Query: 1   MMMECEMEAWISPNGDMSSIDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSL 60
           MM EC MEAWISPNGD S IDLGYGSFGCSHYRRRCKIRAPCCNEIF CRHCHNEAKNSL
Sbjct: 1   MMTECGMEAWISPNGDKSLIDLGYGSFGCSHYRRRCKIRAPCCNEIFYCRHCHNEAKNSL 60

Query: 61  DVDPLLRHDIPRQDMEKVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQY 120
           DVDPLLRHDIPR DMEKVICSLCGTEQDVRQ+CIQCGVCMG+YFCAKCKFFDDDVSKKQY
Sbjct: 61  DVDPLLRHDIPRHDMEKVICSLCGTEQDVRQHCIQCGVCMGRYFCAKCKFFDDDVSKKQY 120

Query: 121 HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS 180
           HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS
Sbjct: 121 HCNECGICRVGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDIS 180

Query: 181 VLPCGHTIHLECVKEMEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSK 240
           VLPCGHTIHLECVKEMEQRLQ                               YTCPVCSK
Sbjct: 181 VLPCGHTIHLECVKEMEQRLQ-------------------------------YTCPVCSK 240

Query: 241 SYCDMSSVWEKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 300
           SYCDMSSVWEKLDHEVASTPMPQ+YQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT
Sbjct: 241 SYCDMSSVWEKLDHEVASTPMPQIYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNT 288

Query: 301 RLTQGGPSSCSPRIAQIVR 319
           RLTQGGPSSCS RI QIVR
Sbjct: 301 RLTQGGPSSCSQRIPQIVR 288

BLAST of Chy8G154840 vs. TAIR 10
Match: AT5G22920.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 426.8 bits (1096), Expect = 1.5e-119
Identity = 188/301 (62.46%), Postives = 228/301 (75.75%), Query Frame = 0

Query: 20  IDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRQDMEKVI 79
           +++G G +GCSHYRRRCKIRAPCC+EIFDCRHCHNEAK+SL ++   RH++PR ++ KVI
Sbjct: 18  MEIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRHELPRHEVSKVI 77

Query: 80  CSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEDNFFHC 139
           CSLC TEQDV+Q C  CGVCMGKYFC+KCKFFDDD+SKKQYHC+ECGICR GGE+NFFHC
Sbjct: 78  CSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGICRTGGEENFFHC 137

Query: 140 KKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISVLPCGHTIHLECVKEMEQR 199
           K+C CCYSK+ME+ H CVE AMHHNCPVCFE+LFD+  DI+VL CGHT+HLEC K+M   
Sbjct: 138 KRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTMHLECTKDMG-- 197

Query: 200 LQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSKSYCDMSSVWEKLDHEVAST 259
                           L++             RYTCPVCSKS CDMS++W+KLD EVA+ 
Sbjct: 198 ----------------LHN-------------RYTCPVCSKSICDMSNLWKKLDEEVAAY 257

Query: 260 PMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTRLTQGGPS--SCSPRIAQI 319
           PMP+MY++KMVWILCNDC   + V +H++AHKC  C SYNTR TQ G    SCS  + Q+
Sbjct: 258 PMPKMYENKMVWILCNDCGSNTNVRFHLIAHKCSSCGSYNTRQTQRGSDSHSCSSGMPQV 287

BLAST of Chy8G154840 vs. TAIR 10
Match: AT5G25560.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 396.7 bits (1018), Expect = 1.7e-110
Identity = 172/286 (60.14%), Postives = 206/286 (72.03%), Query Frame = 0

Query: 20  IDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRQDMEKVI 79
           +D G   +GC HYRRRC IRAPCCNEIF C HCH EAKN+++VD   RHDIPR  +E+VI
Sbjct: 54  LDRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQVI 113

Query: 80  CSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEDNFFHC 139
           C LCGTEQ+V Q CI CGVCMGKYFC  CK +DDD SKKQYHC+ CGICR+GG +NFFHC
Sbjct: 114 CLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRIGGRENFFHC 173

Query: 140 KKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISVLPCGHTIHLECVKEMEQR 199
            KCGCCYS L++N H CVE AMHH+CP+CFEFLF++ +D++VLPCGHTIH +C++EM   
Sbjct: 174 YKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQKCLEEMRDH 233

Query: 200 LQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSKSYCDMSSVWEKLDHEVAST 259
            Q                               Y CP+CSKS CDMS VWEK D E+A+T
Sbjct: 234 YQ-------------------------------YACPLCSKSVCDMSKVWEKFDMEIAAT 293

Query: 260 PMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTRLTQG 306
           PMP+ YQ++MV ILCNDC + +EV YH+VA KC  CKSYNTR T+G
Sbjct: 294 PMPEPYQNRMVQILCNDCGKKAEVQYHVVAQKCPNCKSYNTRQTRG 308

BLAST of Chy8G154840 vs. TAIR 10
Match: AT5G25560.3 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 388.7 bits (997), Expect = 4.7e-108
Identity = 172/296 (58.11%), Postives = 206/296 (69.59%), Query Frame = 0

Query: 20  IDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRQDME--- 79
           +D G   +GC HYRRRC IRAPCCNEIF C HCH EAKN+++VD   RHDIPR  +E   
Sbjct: 54  LDRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQLT 113

Query: 80  -------KVICSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICR 139
                  +VIC LCGTEQ+V Q CI CGVCMGKYFC  CK +DDD SKKQYHC+ CGICR
Sbjct: 114 RPLSSSSQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICR 173

Query: 140 VGGEDNFFHCKKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISVLPCGHTIH 199
           +GG +NFFHC KCGCCYS L++N H CVE AMHH+CP+CFEFLF++ +D++VLPCGHTIH
Sbjct: 174 IGGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIH 233

Query: 200 LECVKEMEQRLQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSKSYCDMSSVW 259
            +C++EM    Q                               Y CP+CSKS CDMS VW
Sbjct: 234 QKCLEEMRDHYQ-------------------------------YACPLCSKSVCDMSKVW 293

Query: 260 EKLDHEVASTPMPQMYQHKMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTRLTQG 306
           EK D E+A+TPMP+ YQ++MV ILCNDC + +EV YH+VA KC  CKSYNTR T+G
Sbjct: 294 EKFDMEIAATPMPEPYQNRMVQILCNDCGKKAEVQYHVVAQKCPNCKSYNTRQTRG 318

BLAST of Chy8G154840 vs. TAIR 10
Match: AT5G25560.4 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 382.5 bits (981), Expect = 3.4e-106
Identity = 167/278 (60.07%), Postives = 199/278 (71.58%), Query Frame = 0

Query: 28  GCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRQDMEKVICSLCGTEQ 87
           G   Y RRC IRAPCCNEIF C HCH EAKN+++VD   RHDIPR  +E+VIC LCGTEQ
Sbjct: 57  GLMEYGRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQVICLLCGTEQ 116

Query: 88  DVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEDNFFHCKKCGCCYS 147
           +V Q CI CGVCMGKYFC  CK +DDD SKKQYHC+ CGICR+GG +NFFHC KCGCCYS
Sbjct: 117 EVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRIGGRENFFHCYKCGCCYS 176

Query: 148 KLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISVLPCGHTIHLECVKEMEQRLQLGHLFF 207
            L++N H CVE AMHH+CP+CFEFLF++ +D++VLPCGHTIH +C++EM    Q      
Sbjct: 177 ILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQKCLEEMRDHYQ------ 236

Query: 208 LLRKILFVLYDHLNELPKPLLCICRYTCPVCSKSYCDMSSVWEKLDHEVASTPMPQMYQH 267
                                    Y CP+CSKS CDMS VWEK D E+A+TPMP+ YQ+
Sbjct: 237 -------------------------YACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQN 296

Query: 268 KMVWILCNDCSEISEVNYHIVAHKCLKCKSYNTRLTQG 306
           +MV ILCNDC + +EV YH+VA KC  CKSYNTR T+G
Sbjct: 297 RMVQILCNDCGKKAEVQYHVVAQKCPNCKSYNTRQTRG 303

BLAST of Chy8G154840 vs. TAIR 10
Match: AT5G25560.2 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 345.1 bits (884), Expect = 5.9e-95
Identity = 149/251 (59.36%), Postives = 179/251 (71.31%), Query Frame = 0

Query: 20  IDLGYGSFGCSHYRRRCKIRAPCCNEIFDCRHCHNEAKNSLDVDPLLRHDIPRQDMEKVI 79
           +D G   +GC HYRRRC IRAPCCNEIF C HCH EAKN+++VD   RHDIPR  +E+VI
Sbjct: 54  LDRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQVI 113

Query: 80  CSLCGTEQDVRQYCIQCGVCMGKYFCAKCKFFDDDVSKKQYHCNECGICRVGGEDNFFHC 139
           C LCGTEQ+V Q CI CGVCMGKYFC  CK +DDD SKKQYHC+ CGICR+GG +NFFHC
Sbjct: 114 CLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRIGGRENFFHC 173

Query: 140 KKCGCCYSKLMENSHNCVEKAMHHNCPVCFEFLFDTMSDISVLPCGHTIHLECVKEMEQR 199
            KCGCCYS L++N H CVE AMHH+CP+CFEFLF++ +D++VLPCGHTIH +C++EM   
Sbjct: 174 YKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQKCLEEMRDH 233

Query: 200 LQLGHLFFLLRKILFVLYDHLNELPKPLLCICRYTCPVCSKSYCDMSSVWEKLDHEVAST 259
            Q                               Y CP+CSKS CDMS VWEK D E+A+T
Sbjct: 234 YQ-------------------------------YACPLCSKSVCDMSKVWEKFDMEIAAT 273

Query: 260 PMPQMYQHKMV 271
           PMP+ YQ++MV
Sbjct: 294 PMPEPYQNRMV 273

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FFB62.2e-11862.46E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=... [more]
Q5JL968.3e-11058.74Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=3... [more]
Q8VZK02.7e-9250.17E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 ... [more]
Q10LI11.4e-7745.61E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SR... [more]
Q96PM53.7e-5738.43RING finger and CHY zinc finger domain-containing protein 1 OS=Homo sapiens OX=9... [more]
Match NameE-valueIdentityDescription
A0A0A0KIG32.4e-17690.28Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G448100 PE=4 SV=1[more]
A0A5A7V9A02.9e-17489.62E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CHL25.0e-17489.34E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo OX=3656 GN=LOC103500836 P... [more]
A0A6J1I9L51.6e-16484.64E3 ubiquitin-protein ligase RZFP34-like OS=Cucurbita maxima OX=3661 GN=LOC111471... [more]
A0A6J1HG842.1e-16484.01E3 ubiquitin-protein ligase RZFP34-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
XP_004143281.15.21e-22490.28E3 ubiquitin-protein ligase RZFP34 isoform X1 [Cucumis sativus] >XP_011657675.1 ... [more]
KAA0063794.12.79e-22189.62E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo var. makuwa][more]
XP_008462499.15.84e-22189.34PREDICTED: E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo] >XP_008462500.1... [more]
XP_031743612.19.00e-21788.71E3 ubiquitin-protein ligase RZFP34 isoform X2 [Cucumis sativus] >XP_031743613.1 ... [more]
XP_038882721.12.98e-21486.83E3 ubiquitin-protein ligase RZFP34-like [Benincasa hispida] >XP_038882722.1 E3 u... [more]
Match NameE-valueIdentityDescription
AT5G22920.11.5e-11962.46CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.11.7e-11060.14CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.34.7e-10858.11CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.43.4e-10660.07CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.25.9e-9559.36CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 165..238
e-value: 1.3E-4
score: 31.3
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 165..239
score: 9.686208
IPR008913Zinc finger, CHY-typePFAMPF05495zf-CHYcoord: 29..110
e-value: 1.9E-18
score: 66.7
IPR008913Zinc finger, CHY-typePROSITEPS51266ZF_CHYcoord: 22..98
score: 24.270576
IPR027370RING-type zinc-finger, LisH dimerisation motifPFAMPF13445zf-RING_UBOXcoord: 165..199
e-value: 9.6E-7
score: 28.7
NoneNo IPR availableGENE3D2.20.28.10coord: 269..308
e-value: 1.1E-17
score: 64.9
NoneNo IPR availablePANTHERPTHR21319RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1coord: 3..201
coord: 230..317
NoneNo IPR availablePANTHERPTHR21319:SF54E3 UBIQUITIN-PROTEIN LIGASE RZFP34coord: 3..201
NoneNo IPR availablePANTHERPTHR21319:SF54E3 UBIQUITIN-PROTEIN LIGASE RZFP34coord: 230..317
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 164..197
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 145..268
e-value: 9.5E-9
score: 36.7
IPR039512RCHY1, zinc-ribbonPFAMPF14599zinc_ribbon_6coord: 243..301
e-value: 2.2E-25
score: 88.4
IPR017921Zinc finger, CTCHY-typePROSITEPS51270ZF_CTCHYcoord: 100..164
score: 34.232605
IPR037274Zinc finger, CHY-type superfamilySUPERFAMILY161219CHY zinc finger-likecoord: 26..100
IPR037275Zinc finger, CTCHY-type superfamilySUPERFAMILY161245Zinc hairpin stackcoord: 101..158

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy8G154840.1Chy8G154840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding