Chy8G154380 (gene) Cucumber (hystrix) v1

Overview
NameChy8G154380
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionaquaporin PIP2-2-like
LocationchrH08: 15840346 .. 15841472 (+)
RNA-Seq ExpressionChy8G154380
SyntenyChy8G154380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTAAGGATCTTGAAGCTGGTGGATTCGCCGTCAAGGATTACCAAGACCCACCTCCAGCTCCATTGATCGATGCCGAAGAGTTAACTCAATGGTCTTTTTACAGAGCTATCATTGCCGAGTTCGTCGCCACTCTTTTGTTCTTGTACGTCACCGTTCTCACTGTCATTGGCTACAGTAGCCAATCCGACACTAAAAACGGCGGCCAAATCTGTGGCGGCGTCGGCATTCTTGGCATTGCTTGGGCATTTGGTGGCATGATCTTTGTTCTCGTTTACTGCACTGCTGGAATTTCCGGTGCGTATGAGTTTTTTTTTGGGATCAATTGCTCTGTTTCTCTCTCTGTTCATTCTGTTTATAGAAAATTACCTTCTTTTTTTTTTTTATTGTAGGTGGCCATATTAACCCAGCGGTGACTTTTGGGTTGTTCTTGGCGCGGAAAGTGTCTTTCATTAGAGCAATTCTTTACATGGTGGCTCAATGTTTGGGCGCCATTTGTGGCTGCGCTTTGGTGAAATCATTCCAGAAGGCTCTTTACAATGAGCACGGAGGTGGAGCCAATTCTCTCGCGGATGGGTACAGCACCGGCACCGGCTTAGCCGCGGAGATAATCGGAACTTTCGTTCTTGTTTACACCGTCTTCTCCGCCACAGATCCCAAGAGAAATGCTAGAGATTCTCACGTTCCTGTAAGTTCATTCAATTCCCTAAAATTGGTTTAACCATTCACCCTCATGTCACTCGATCGACCTAACTAAATTTTGTTTCGAAATTGAAGGTTTTGGCTCCACTCCCAATTGGGTTCGCGGTGTTTATGGTTCATTTGGCCACCATTCCTATCACCGGCACCGGAATCAACCCAGCTCGAAGTTTTGGAGCTGCCGTGATCTTTAACAAAGACAAGCCATGGGATGACCAGGTAACAAAATCAATCAGTCTCTTTCAGCATTTGAACTTCGTTTTAATGTAATCCAACAATTGAAACTTATTTCTTTGTATGTGGGCAGTGGATATTTTGGGTTGGACCGTTCATTGGAGCTGCGATTGCTGCAATTTATCACCAGGTTGTGTTGAGGGCAGGAGCAGTTAAAGCTCTGGGATCGTTCAGAAGTTCGACTGCCGTATGA

mRNA sequence

ATGTCTAAGGATCTTGAAGCTGGTGGATTCGCCGTCAAGGATTACCAAGACCCACCTCCAGCTCCATTGATCGATGCCGAAGAGTTAACTCAATGGTCTTTTTACAGAGCTATCATTGCCGAGTTCGTCGCCACTCTTTTGTTCTTGTACGTCACCGTTCTCACTGTCATTGGCTACAGTAGCCAATCCGACACTAAAAACGGCGGCCAAATCTGTGGCGGCGTCGGCATTCTTGGCATTGCTTGGGCATTTGGTGGCATGATCTTTGTTCTCGTTTACTGCACTGCTGGAATTTCCGGTGGCCATATTAACCCAGCGGTGACTTTTGGGTTGTTCTTGGCGCGGAAAGTGTCTTTCATTAGAGCAATTCTTTACATGGTGGCTCAATGTTTGGGCGCCATTTGTGGCTGCGCTTTGGTGAAATCATTCCAGAAGGCTCTTTACAATGAGCACGGAGGTGGAGCCAATTCTCTCGCGGATGGGTACAGCACCGGCACCGGCTTAGCCGCGGAGATAATCGGAACTTTCGTTCTTGTTTACACCGTCTTCTCCGCCACAGATCCCAAGAGAAATGCTAGAGATTCTCACGTTCCTGTTTTGGCTCCACTCCCAATTGGGTTCGCGGTGTTTATGGTTCATTTGGCCACCATTCCTATCACCGGCACCGGAATCAACCCAGCTCGAAGTTTTGGAGCTGCCGTGATCTTTAACAAAGACAAGCCATGGGATGACCAGTGGATATTTTGGGTTGGACCGTTCATTGGAGCTGCGATTGCTGCAATTTATCACCAGGTTGTGTTGAGGGCAGGAGCAGTTAAAGCTCTGGGATCGTTCAGAAGTTCGACTGCCGTATGA

Coding sequence (CDS)

ATGTCTAAGGATCTTGAAGCTGGTGGATTCGCCGTCAAGGATTACCAAGACCCACCTCCAGCTCCATTGATCGATGCCGAAGAGTTAACTCAATGGTCTTTTTACAGAGCTATCATTGCCGAGTTCGTCGCCACTCTTTTGTTCTTGTACGTCACCGTTCTCACTGTCATTGGCTACAGTAGCCAATCCGACACTAAAAACGGCGGCCAAATCTGTGGCGGCGTCGGCATTCTTGGCATTGCTTGGGCATTTGGTGGCATGATCTTTGTTCTCGTTTACTGCACTGCTGGAATTTCCGGTGGCCATATTAACCCAGCGGTGACTTTTGGGTTGTTCTTGGCGCGGAAAGTGTCTTTCATTAGAGCAATTCTTTACATGGTGGCTCAATGTTTGGGCGCCATTTGTGGCTGCGCTTTGGTGAAATCATTCCAGAAGGCTCTTTACAATGAGCACGGAGGTGGAGCCAATTCTCTCGCGGATGGGTACAGCACCGGCACCGGCTTAGCCGCGGAGATAATCGGAACTTTCGTTCTTGTTTACACCGTCTTCTCCGCCACAGATCCCAAGAGAAATGCTAGAGATTCTCACGTTCCTGTTTTGGCTCCACTCCCAATTGGGTTCGCGGTGTTTATGGTTCATTTGGCCACCATTCCTATCACCGGCACCGGAATCAACCCAGCTCGAAGTTTTGGAGCTGCCGTGATCTTTAACAAAGACAAGCCATGGGATGACCAGTGGATATTTTGGGTTGGACCGTTCATTGGAGCTGCGATTGCTGCAATTTATCACCAGGTTGTGTTGAGGGCAGGAGCAGTTAAAGCTCTGGGATCGTTCAGAAGTTCGACTGCCGTATGA

Protein sequence

MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYSSQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSFIRAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDKPWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV*
Homology
BLAST of Chy8G154380 vs. ExPASy Swiss-Prot
Match: P43287 (Aquaporin PIP2-2 OS=Arabidopsis thaliana OX=3702 GN=PIP2-2 PE=1 SV=2)

HSP 1 Score: 481.5 bits (1238), Expect = 6.6e-135
Identity = 237/285 (83.16%), Postives = 255/285 (89.47%), Query Frame = 0

Query: 1   MSKDLEA-GGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGY 60
           M+KD+E   GF  +DY+DPPP P  DA+ELT+WS YRA+IAEFVATLLFLY+TVLTVIGY
Sbjct: 1   MAKDVEGPEGFQTRDYEDPPPTPFFDADELTKWSLYRAVIAEFVATLLFLYITVLTVIGY 60

Query: 61  SSQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSF 120
             QSDTK GG  CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFLARKVS 
Sbjct: 61  KIQSDTKAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSL 120

Query: 121 IRAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLV 180
           IRA+LYMVAQCLGAICG   VK+FQ + Y+ +GGGANSLADGY+TGTGLAAEIIGTFVLV
Sbjct: 121 IRAVLYMVAQCLGAICGVGFVKAFQSSYYDRYGGGANSLADGYNTGTGLAAEIIGTFVLV 180

Query: 181 YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKD 240
           YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI+NK 
Sbjct: 181 YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKS 240

Query: 241 KPWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 285
           KPWDD WIFWVGPFIGAAIAA YHQ VLRA   K+LGSFRS+  V
Sbjct: 241 KPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 285

BLAST of Chy8G154380 vs. ExPASy Swiss-Prot
Match: P30302 (Aquaporin PIP2-3 OS=Arabidopsis thaliana OX=3702 GN=PIP2-3 PE=1 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 5.6e-134
Identity = 238/285 (83.51%), Postives = 252/285 (88.42%), Query Frame = 0

Query: 1   MSKDLEA-GGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGY 60
           M+KD+E   GF  +DY+DPPP P  DAEELT+WS YRA+IAEFVATLLFLYVTVLTVIGY
Sbjct: 1   MAKDVEGPDGFQTRDYEDPPPTPFFDAEELTKWSLYRAVIAEFVATLLFLYVTVLTVIGY 60

Query: 61  SSQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSF 120
             QSDTK GG  CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFLARKVS 
Sbjct: 61  KIQSDTKAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSL 120

Query: 121 IRAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLV 180
           IRA+LYMVAQCLGAICG   VK+FQ + Y  +GGGAN LADGY+TGTGLAAEIIGTFVLV
Sbjct: 121 IRAVLYMVAQCLGAICGVGFVKAFQSSHYVNYGGGANFLADGYNTGTGLAAEIIGTFVLV 180

Query: 181 YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKD 240
           YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNK 
Sbjct: 181 YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKS 240

Query: 241 KPWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 285
           KPWDD WIFWVGPFIGA IAA YHQ VLRA   K+LGSFRS+  V
Sbjct: 241 KPWDDHWIFWVGPFIGATIAAFYHQFVLRASGSKSLGSFRSAANV 285

BLAST of Chy8G154380 vs. ExPASy Swiss-Prot
Match: P43286 (Aquaporin PIP2-1 OS=Arabidopsis thaliana OX=3702 GN=PIP2-1 PE=1 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 4.8e-133
Identity = 236/287 (82.23%), Postives = 253/287 (88.15%), Query Frame = 0

Query: 1   MSKDLEA---GGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVI 60
           M+KD+EA    GF  +DYQDPPPAP ID  EL +WSFYRA+IAEFVATLLFLY+TVLTVI
Sbjct: 1   MAKDVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVI 60

Query: 61  GYSSQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKV 120
           GY  QSDT  GG  CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFLARKV
Sbjct: 61  GYKIQSDTDAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 121 SFIRAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFV 180
           S  RA+LY++AQCLGAICG   VK+FQ + Y  +GGGANSLADGYSTGTGLAAEIIGTFV
Sbjct: 121 SLPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFV 180

Query: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFN 240
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI+N
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYN 240

Query: 241 KDKPWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 285
           K KPWDD WIFWVGPFIGAAIAA YHQ VLRA   K+LGSFRS+  V
Sbjct: 241 KSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 287

BLAST of Chy8G154380 vs. ExPASy Swiss-Prot
Match: Q84RL7 (Aquaporin PIP2-1 OS=Zea mays OX=4577 GN=PIP2-1 PE=1 SV=2)

HSP 1 Score: 462.6 bits (1189), Expect = 3.2e-129
Identity = 226/276 (81.88%), Postives = 244/276 (88.41%), Query Frame = 0

Query: 8   GGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYSSQSDTKN 67
           G FA KDY DPPPAPLIDA EL  WS YRA+IAEF+ATLLFLY+TV TVIGY  Q+D   
Sbjct: 14  GEFAAKDYTDPPPAPLIDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDASA 73

Query: 68  GG--QICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSFIRAILY 127
            G    CGGVG+LGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVS +RA+LY
Sbjct: 74  SGADAACGGVGVLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRALLY 133

Query: 128 MVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVYTVFSA 187
           +VAQCLGAICG  LVK+FQ A ++ +GGGANSLA GYS GTGL AEIIGTFVLVYTVFSA
Sbjct: 134 IVAQCLGAICGVGLVKAFQSAYFDRYGGGANSLASGYSRGTGLGAEIIGTFVLVYTVFSA 193

Query: 188 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDKPWDDQ 247
           TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIP+TGTGINPARS GAAVI+NKDKPWDD 
Sbjct: 194 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYNKDKPWDDH 253

Query: 248 WIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSS 282
           WIFWVGP +GAAIAA YHQ +LRAGA+KALGSFRS+
Sbjct: 254 WIFWVGPLVGAAIAAFYHQYILRAGAIKALGSFRSN 289

BLAST of Chy8G154380 vs. ExPASy Swiss-Prot
Match: Q9ATM6 (Aquaporin PIP2-4 OS=Zea mays OX=4577 GN=PIP2-4 PE=1 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 5.5e-129
Identity = 232/288 (80.56%), Postives = 250/288 (86.81%), Query Frame = 0

Query: 1   MSKDLEAGG-----FAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLT 60
           M+KD+EA G     F+ KDY DPPPAPLIDAEELTQWS YRA+IAEF+ATLLFLY+TV T
Sbjct: 1   MAKDIEASGPEAGEFSAKDYTDPPPAPLIDAEELTQWSLYRAVIAEFIATLLFLYITVAT 60

Query: 61  VIGYSSQSDTKNGG--QICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFL 120
           VIGY  Q+D    G    CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFL
Sbjct: 61  VIGYKHQTDASASGPDAACGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFL 120

Query: 121 ARKVSFIRAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEII 180
           ARKVS +RA+LY++AQCLGAICG  LVK FQ A Y  +GGGAN L+DGYS GTGLAAEII
Sbjct: 121 ARKVSLVRALLYIIAQCLGAICGVGLVKGFQSAYYVRYGGGANELSDGYSKGTGLAAEII 180

Query: 181 GTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAA 240
           GTFVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARS GAA
Sbjct: 181 GTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAA 240

Query: 241 VIFNKDKPWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSS 282
           VI+NKDK WDDQWIFWVGP IGAAIAA YHQ VLRA A K LGS+RS+
Sbjct: 241 VIYNKDKAWDDQWIFWVGPLIGAAIAAAYHQYVLRASATK-LGSYRSN 287

BLAST of Chy8G154380 vs. ExPASy TrEMBL
Match: V5RDW7 (Plasma intrinsic protein 2-3 OS=Cucumis sativus OX=3659 GN=PIP2-3 PE=2 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.1e-156
Identity = 282/284 (99.30%), Postives = 282/284 (99.30%), Query Frame = 0

Query: 1   MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60
           MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS
Sbjct: 1   MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60

Query: 61  SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSFI 120
           SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVS  
Sbjct: 61  SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLT 120

Query: 121 RAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVY 180
           RAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVY
Sbjct: 121 RAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVY 180

Query: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK 240
           TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK
Sbjct: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK 240

Query: 241 PWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 285
           PWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV
Sbjct: 241 PWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 284

BLAST of Chy8G154380 vs. ExPASy TrEMBL
Match: A0A1S3BL05 (aquaporin PIP2-2-like OS=Cucumis melo OX=3656 GN=LOC107990277 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.5e-153
Identity = 276/284 (97.18%), Postives = 280/284 (98.59%), Query Frame = 0

Query: 1   MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60
           MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS
Sbjct: 1   MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60

Query: 61  SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSFI 120
           SQSDTK+GGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVS I
Sbjct: 61  SQSDTKSGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLI 120

Query: 121 RAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVY 180
           RAILYM AQCLGAICGCALVKSFQKALYN +GGGANSLADGYSTGTGLAAEIIGTFVLVY
Sbjct: 121 RAILYMAAQCLGAICGCALVKSFQKALYNGYGGGANSLADGYSTGTGLAAEIIGTFVLVY 180

Query: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK 240
           TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI+NKDK
Sbjct: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKDK 240

Query: 241 PWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 285
            WDDQWIFWVGPFIGAAIAAIYHQ+VLRAGAVKALGSFRSSTAV
Sbjct: 241 AWDDQWIFWVGPFIGAAIAAIYHQIVLRAGAVKALGSFRSSTAV 284

BLAST of Chy8G154380 vs. ExPASy TrEMBL
Match: A0A6J1ISY0 (aquaporin PIP2-2-like OS=Cucurbita maxima OX=3661 GN=LOC111478388 PE=3 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 1.3e-152
Identity = 272/284 (95.77%), Postives = 278/284 (97.89%), Query Frame = 0

Query: 1   MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60
           MSKDLEAGGFA KDYQDPPPAPLIDA+E TQWSFYRAIIAEFVATLLFLYVTVLTVIGYS
Sbjct: 1   MSKDLEAGGFATKDYQDPPPAPLIDADEFTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60

Query: 61  SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSFI 120
           +QSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVS +
Sbjct: 61  NQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLV 120

Query: 121 RAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVY 180
           RAILYM AQCLGAICGCALVKSFQKA YNE+GGGANSLADGYSTGTGLAAEIIGTFVLVY
Sbjct: 121 RAILYMAAQCLGAICGCALVKSFQKAHYNEYGGGANSLADGYSTGTGLAAEIIGTFVLVY 180

Query: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK 240
           TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI NKDK
Sbjct: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVILNKDK 240

Query: 241 PWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 285
           PWDDQWIFWVGPFIGAAIAAIYHQ++LRAGAVKALGSFRSSTAV
Sbjct: 241 PWDDQWIFWVGPFIGAAIAAIYHQIILRAGAVKALGSFRSSTAV 284

BLAST of Chy8G154380 vs. ExPASy TrEMBL
Match: A0A6J1EJX2 (aquaporin PIP2-2-like OS=Cucurbita moschata OX=3662 GN=LOC111433285 PE=3 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 2.8e-152
Identity = 271/284 (95.42%), Postives = 278/284 (97.89%), Query Frame = 0

Query: 1   MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60
           MSKDLEAGGFA KDYQDPPPAPLIDA+E TQWSFYRAIIAEFVATLLFLYVTVLTVIGYS
Sbjct: 1   MSKDLEAGGFATKDYQDPPPAPLIDADEFTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60

Query: 61  SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSFI 120
           +QSD KNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVS +
Sbjct: 61  NQSDIKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLV 120

Query: 121 RAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVY 180
           RAILYM AQCLGAICGCALVKSFQKA YNE+GGGANSLADGYSTGTGLAAEIIGTFVLVY
Sbjct: 121 RAILYMAAQCLGAICGCALVKSFQKAHYNEYGGGANSLADGYSTGTGLAAEIIGTFVLVY 180

Query: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK 240
           TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK
Sbjct: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK 240

Query: 241 PWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 285
           PWDDQWIFWVGPFIGAAIAAIYHQ++LRAGAVKALGSFRSST+V
Sbjct: 241 PWDDQWIFWVGPFIGAAIAAIYHQIILRAGAVKALGSFRSSTSV 284

BLAST of Chy8G154380 vs. ExPASy TrEMBL
Match: A0A6J1CQG7 (aquaporin PIP2-2-like OS=Momordica charantia OX=3673 GN=LOC111013638 PE=3 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 1.8e-143
Identity = 256/284 (90.14%), Postives = 267/284 (94.01%), Query Frame = 0

Query: 1   MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60
           M+KD EAGGFA KDY DPPPAPLIDA+E TQWSFYRAIIAEF+ATLLFLYVTVLTVIGYS
Sbjct: 1   MAKDSEAGGFAAKDYHDPPPAPLIDAQEFTQWSFYRAIIAEFIATLLFLYVTVLTVIGYS 60

Query: 61  SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSFI 120
            QSDTKNGG+ICGGVGILG+AWAFGGMIFVLVYCTAGISGGHINPAVTFGL LARKVS +
Sbjct: 61  HQSDTKNGGEICGGVGILGVAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLV 120

Query: 121 RAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVY 180
           RAILYMVAQ LGAICGCALVKSFQK LY+ + GGAN LADGYSTGTGLAAEIIGTFVLVY
Sbjct: 121 RAILYMVAQSLGAICGCALVKSFQKGLYHRYNGGANQLADGYSTGTGLAAEIIGTFVLVY 180

Query: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK 240
           TVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI NK K
Sbjct: 181 TVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIINKSK 240

Query: 241 PWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 285
           PWDDQWIFWVGPFIGAAIAA YHQ +LRAGAVKALGSFRSS +V
Sbjct: 241 PWDDQWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSSHSV 284

BLAST of Chy8G154380 vs. NCBI nr
Match: NP_001292671.1 (aquaporin PIP2-2 [Cucumis sativus] >AHB33479.1 plasma intrinsic protein 2-3 [Cucumis sativus] >KGN48149.1 hypothetical protein Csa_002998 [Cucumis sativus])

HSP 1 Score: 565 bits (1456), Expect = 4.86e-203
Identity = 282/284 (99.30%), Postives = 282/284 (99.30%), Query Frame = 0

Query: 1   MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60
           MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS
Sbjct: 1   MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60

Query: 61  SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSFI 120
           SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVS  
Sbjct: 61  SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLT 120

Query: 121 RAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVY 180
           RAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVY
Sbjct: 121 RAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVY 180

Query: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK 240
           TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK
Sbjct: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK 240

Query: 241 PWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 284
           PWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV
Sbjct: 241 PWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 284

BLAST of Chy8G154380 vs. NCBI nr
Match: XP_038880916.1 (aquaporin PIP2-2-like [Benincasa hispida])

HSP 1 Score: 558 bits (1437), Expect = 3.83e-200
Identity = 276/284 (97.18%), Postives = 280/284 (98.59%), Query Frame = 0

Query: 1   MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60
           MSKDLEAGGFA KDYQDPPPAPLIDAEE TQWSFYRAIIAEFVATLLFLYVTVLTVIGYS
Sbjct: 1   MSKDLEAGGFAAKDYQDPPPAPLIDAEEFTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60

Query: 61  SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSFI 120
           SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVS +
Sbjct: 61  SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLV 120

Query: 121 RAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVY 180
           RAILYMVAQCLGAICGCALVKSFQKA YNE+GGGANSLADGYSTGTGLAAEIIGTFVLVY
Sbjct: 121 RAILYMVAQCLGAICGCALVKSFQKAHYNEYGGGANSLADGYSTGTGLAAEIIGTFVLVY 180

Query: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK 240
           TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAV+FNKDK
Sbjct: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVVFNKDK 240

Query: 241 PWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 284
           PWDDQWIFWVGPFIGAAIAAIYHQ+VLRAGAVKALGSFRSSTAV
Sbjct: 241 PWDDQWIFWVGPFIGAAIAAIYHQIVLRAGAVKALGSFRSSTAV 284

BLAST of Chy8G154380 vs. NCBI nr
Match: NP_001380708.1 (aquaporin PIP2-3 [Cucumis melo])

HSP 1 Score: 554 bits (1427), Expect = 1.28e-198
Identity = 276/284 (97.18%), Postives = 280/284 (98.59%), Query Frame = 0

Query: 1   MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60
           MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS
Sbjct: 1   MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60

Query: 61  SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSFI 120
           SQSDTK+GGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVS I
Sbjct: 61  SQSDTKSGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLI 120

Query: 121 RAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVY 180
           RAILYM AQCLGAICGCALVKSFQKALYN +GGGANSLADGYSTGTGLAAEIIGTFVLVY
Sbjct: 121 RAILYMAAQCLGAICGCALVKSFQKALYNGYGGGANSLADGYSTGTGLAAEIIGTFVLVY 180

Query: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK 240
           TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI+NKDK
Sbjct: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKDK 240

Query: 241 PWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 284
            WDDQWIFWVGPFIGAAIAAIYHQ+VLRAGAVKALGSFRSSTAV
Sbjct: 241 AWDDQWIFWVGPFIGAAIAAIYHQIVLRAGAVKALGSFRSSTAV 284

BLAST of Chy8G154380 vs. NCBI nr
Match: XP_022978378.1 (aquaporin PIP2-2-like [Cucurbita maxima])

HSP 1 Score: 551 bits (1421), Expect = 1.05e-197
Identity = 272/284 (95.77%), Postives = 278/284 (97.89%), Query Frame = 0

Query: 1   MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60
           MSKDLEAGGFA KDYQDPPPAPLIDA+E TQWSFYRAIIAEFVATLLFLYVTVLTVIGYS
Sbjct: 1   MSKDLEAGGFATKDYQDPPPAPLIDADEFTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60

Query: 61  SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSFI 120
           +QSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVS +
Sbjct: 61  NQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLV 120

Query: 121 RAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVY 180
           RAILYM AQCLGAICGCALVKSFQKA YNE+GGGANSLADGYSTGTGLAAEIIGTFVLVY
Sbjct: 121 RAILYMAAQCLGAICGCALVKSFQKAHYNEYGGGANSLADGYSTGTGLAAEIIGTFVLVY 180

Query: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK 240
           TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI NKDK
Sbjct: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVILNKDK 240

Query: 241 PWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 284
           PWDDQWIFWVGPFIGAAIAAIYHQ++LRAGAVKALGSFRSSTAV
Sbjct: 241 PWDDQWIFWVGPFIGAAIAAIYHQIILRAGAVKALGSFRSSTAV 284

BLAST of Chy8G154380 vs. NCBI nr
Match: XP_022926050.1 (aquaporin PIP2-2-like [Cucurbita moschata] >KAG6604426.1 Aquaporin PIP2-2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034572.1 Aquaporin PIP2-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 550 bits (1418), Expect = 3.02e-197
Identity = 271/284 (95.42%), Postives = 278/284 (97.89%), Query Frame = 0

Query: 1   MSKDLEAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60
           MSKDLEAGGFA KDYQDPPPAPLIDA+E TQWSFYRAIIAEFVATLLFLYVTVLTVIGYS
Sbjct: 1   MSKDLEAGGFATKDYQDPPPAPLIDADEFTQWSFYRAIIAEFVATLLFLYVTVLTVIGYS 60

Query: 61  SQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSFI 120
           +QSD KNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVS +
Sbjct: 61  NQSDIKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLV 120

Query: 121 RAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLVY 180
           RAILYM AQCLGAICGCALVKSFQKA YNE+GGGANSLADGYSTGTGLAAEIIGTFVLVY
Sbjct: 121 RAILYMAAQCLGAICGCALVKSFQKAHYNEYGGGANSLADGYSTGTGLAAEIIGTFVLVY 180

Query: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK 240
           TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK
Sbjct: 181 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKDK 240

Query: 241 PWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 284
           PWDDQWIFWVGPFIGAAIAAIYHQ++LRAGAVKALGSFRSST+V
Sbjct: 241 PWDDQWIFWVGPFIGAAIAAIYHQIILRAGAVKALGSFRSSTSV 284

BLAST of Chy8G154380 vs. TAIR 10
Match: AT2G37170.1 (plasma membrane intrinsic protein 2 )

HSP 1 Score: 481.5 bits (1238), Expect = 4.7e-136
Identity = 237/285 (83.16%), Postives = 255/285 (89.47%), Query Frame = 0

Query: 1   MSKDLEA-GGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGY 60
           M+KD+E   GF  +DY+DPPP P  DA+ELT+WS YRA+IAEFVATLLFLY+TVLTVIGY
Sbjct: 1   MAKDVEGPEGFQTRDYEDPPPTPFFDADELTKWSLYRAVIAEFVATLLFLYITVLTVIGY 60

Query: 61  SSQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSF 120
             QSDTK GG  CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFLARKVS 
Sbjct: 61  KIQSDTKAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSL 120

Query: 121 IRAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLV 180
           IRA+LYMVAQCLGAICG   VK+FQ + Y+ +GGGANSLADGY+TGTGLAAEIIGTFVLV
Sbjct: 121 IRAVLYMVAQCLGAICGVGFVKAFQSSYYDRYGGGANSLADGYNTGTGLAAEIIGTFVLV 180

Query: 181 YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKD 240
           YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI+NK 
Sbjct: 181 YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKS 240

Query: 241 KPWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 285
           KPWDD WIFWVGPFIGAAIAA YHQ VLRA   K+LGSFRS+  V
Sbjct: 241 KPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 285

BLAST of Chy8G154380 vs. TAIR 10
Match: AT2G37180.1 (Aquaporin-like superfamily protein )

HSP 1 Score: 478.4 bits (1230), Expect = 4.0e-135
Identity = 238/285 (83.51%), Postives = 252/285 (88.42%), Query Frame = 0

Query: 1   MSKDLEA-GGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVIGY 60
           M+KD+E   GF  +DY+DPPP P  DAEELT+WS YRA+IAEFVATLLFLYVTVLTVIGY
Sbjct: 1   MAKDVEGPDGFQTRDYEDPPPTPFFDAEELTKWSLYRAVIAEFVATLLFLYVTVLTVIGY 60

Query: 61  SSQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSF 120
             QSDTK GG  CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFLARKVS 
Sbjct: 61  KIQSDTKAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSL 120

Query: 121 IRAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFVLV 180
           IRA+LYMVAQCLGAICG   VK+FQ + Y  +GGGAN LADGY+TGTGLAAEIIGTFVLV
Sbjct: 121 IRAVLYMVAQCLGAICGVGFVKAFQSSHYVNYGGGANFLADGYNTGTGLAAEIIGTFVLV 180

Query: 181 YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKD 240
           YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNK 
Sbjct: 181 YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKS 240

Query: 241 KPWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 285
           KPWDD WIFWVGPFIGA IAA YHQ VLRA   K+LGSFRS+  V
Sbjct: 241 KPWDDHWIFWVGPFIGATIAAFYHQFVLRASGSKSLGSFRSAANV 285

BLAST of Chy8G154380 vs. TAIR 10
Match: AT3G53420.1 (plasma membrane intrinsic protein 2A )

HSP 1 Score: 475.3 bits (1222), Expect = 3.4e-134
Identity = 236/287 (82.23%), Postives = 253/287 (88.15%), Query Frame = 0

Query: 1   MSKDLEA---GGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVI 60
           M+KD+EA    GF  +DYQDPPPAP ID  EL +WSFYRA+IAEFVATLLFLY+TVLTVI
Sbjct: 1   MAKDVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVI 60

Query: 61  GYSSQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKV 120
           GY  QSDT  GG  CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFLARKV
Sbjct: 61  GYKIQSDTDAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 121 SFIRAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFV 180
           S  RA+LY++AQCLGAICG   VK+FQ + Y  +GGGANSLADGYSTGTGLAAEIIGTFV
Sbjct: 121 SLPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFV 180

Query: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFN 240
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI+N
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYN 240

Query: 241 KDKPWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 285
           K KPWDD WIFWVGPFIGAAIAA YHQ VLRA   K+LGSFRS+  V
Sbjct: 241 KSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 287

BLAST of Chy8G154380 vs. TAIR 10
Match: AT3G53420.2 (plasma membrane intrinsic protein 2A )

HSP 1 Score: 475.3 bits (1222), Expect = 3.4e-134
Identity = 236/287 (82.23%), Postives = 253/287 (88.15%), Query Frame = 0

Query: 1   MSKDLEA---GGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVI 60
           M+KD+EA    GF  +DYQDPPPAP ID  EL +WSFYRA+IAEFVATLLFLY+TVLTVI
Sbjct: 1   MAKDVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVI 60

Query: 61  GYSSQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKV 120
           GY  QSDT  GG  CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFLARKV
Sbjct: 61  GYKIQSDTDAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 121 SFIRAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFV 180
           S  RA+LY++AQCLGAICG   VK+FQ + Y  +GGGANSLADGYSTGTGLAAEIIGTFV
Sbjct: 121 SLPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFV 180

Query: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFN 240
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI+N
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYN 240

Query: 241 KDKPWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRSSTAV 285
           K KPWDD WIFWVGPFIGAAIAA YHQ VLRA   K+LGSFRS+  V
Sbjct: 241 KSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 287

BLAST of Chy8G154380 vs. TAIR 10
Match: AT5G60660.1 (plasma membrane intrinsic protein 2;4 )

HSP 1 Score: 456.8 bits (1174), Expect = 1.2e-128
Identity = 224/283 (79.15%), Postives = 245/283 (86.57%), Query Frame = 0

Query: 1   MSKDL---EAGGFAVKDYQDPPPAPLIDAEELTQWSFYRAIIAEFVATLLFLYVTVLTVI 60
           M+KDL   E+G  A +DY+DPPPAP  D EEL +W  YRA+IAEFVATLLFLYV++LTVI
Sbjct: 1   MAKDLDVNESGPPAARDYKDPPPAPFFDMEELRKWPLYRAVIAEFVATLLFLYVSILTVI 60

Query: 61  GYSSQSDTKNGGQICGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKV 120
           GY +Q+D   GG  CGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVT GLFLARKV
Sbjct: 61  GYKAQTDATAGGVDCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTVGLFLARKV 120

Query: 121 SFIRAILYMVAQCLGAICGCALVKSFQKALYNEHGGGANSLADGYSTGTGLAAEIIGTFV 180
           S +R +LY+VAQCLGAICGC  VK+FQ + Y  +GGGAN LADGY+ GTGL AEIIGTFV
Sbjct: 121 SLVRTVLYIVAQCLGAICGCGFVKAFQSSYYTRYGGGANELADGYNKGTGLGAEIIGTFV 180

Query: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFN 240
           LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI+N
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYN 240

Query: 241 KDKPWDDQWIFWVGPFIGAAIAAIYHQVVLRAGAVKALGSFRS 281
            +K WDDQWIFWVGP IGAA AA YHQ +LRA A+KALGSF S
Sbjct: 241 NEKAWDDQWIFWVGPMIGAAAAAFYHQFILRAAAIKALGSFGS 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P432876.6e-13583.16Aquaporin PIP2-2 OS=Arabidopsis thaliana OX=3702 GN=PIP2-2 PE=1 SV=2[more]
P303025.6e-13483.51Aquaporin PIP2-3 OS=Arabidopsis thaliana OX=3702 GN=PIP2-3 PE=1 SV=1[more]
P432864.8e-13382.23Aquaporin PIP2-1 OS=Arabidopsis thaliana OX=3702 GN=PIP2-1 PE=1 SV=1[more]
Q84RL73.2e-12981.88Aquaporin PIP2-1 OS=Zea mays OX=4577 GN=PIP2-1 PE=1 SV=2[more]
Q9ATM65.5e-12980.56Aquaporin PIP2-4 OS=Zea mays OX=4577 GN=PIP2-4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
V5RDW71.1e-15699.30Plasma intrinsic protein 2-3 OS=Cucumis sativus OX=3659 GN=PIP2-3 PE=2 SV=1[more]
A0A1S3BL052.5e-15397.18aquaporin PIP2-2-like OS=Cucumis melo OX=3656 GN=LOC107990277 PE=3 SV=1[more]
A0A6J1ISY01.3e-15295.77aquaporin PIP2-2-like OS=Cucurbita maxima OX=3661 GN=LOC111478388 PE=3 SV=1[more]
A0A6J1EJX22.8e-15295.42aquaporin PIP2-2-like OS=Cucurbita moschata OX=3662 GN=LOC111433285 PE=3 SV=1[more]
A0A6J1CQG71.8e-14390.14aquaporin PIP2-2-like OS=Momordica charantia OX=3673 GN=LOC111013638 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
NP_001292671.14.86e-20399.30aquaporin PIP2-2 [Cucumis sativus] >AHB33479.1 plasma intrinsic protein 2-3 [Cuc... [more]
XP_038880916.13.83e-20097.18aquaporin PIP2-2-like [Benincasa hispida][more]
NP_001380708.11.28e-19897.18aquaporin PIP2-3 [Cucumis melo][more]
XP_022978378.11.05e-19795.77aquaporin PIP2-2-like [Cucurbita maxima][more]
XP_022926050.13.02e-19795.42aquaporin PIP2-2-like [Cucurbita moschata] >KAG6604426.1 Aquaporin PIP2-2, parti... [more]
Match NameE-valueIdentityDescription
AT2G37170.14.7e-13683.16plasma membrane intrinsic protein 2 [more]
AT2G37180.14.0e-13583.51Aquaporin-like superfamily protein [more]
AT3G53420.13.4e-13482.23plasma membrane intrinsic protein 2A [more]
AT3G53420.23.4e-13482.23plasma membrane intrinsic protein 2A [more]
AT5G60660.11.2e-12879.15plasma membrane intrinsic protein 2;4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 206..228
score: 58.26
coord: 84..108
score: 62.61
coord: 246..266
score: 50.53
coord: 170..188
score: 54.14
coord: 121..140
score: 50.67
coord: 36..55
score: 43.81
IPR000425Major intrinsic proteinTIGRFAMTIGR00861TIGR00861coord: 40..262
e-value: 1.8E-64
score: 215.6
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 28..263
e-value: 3.3E-85
score: 285.4
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 36..266
e-value: 3.42519E-74
score: 224.825
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 21..284
e-value: 6.0E-89
score: 299.5
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 28..268
NoneNo IPR availablePANTHERPTHR45687:SF64PLASMAMEMBRANE INTRINSIC PROTEIN 25coord: 2..281
IPR034294Aquaporin transporterPANTHERPTHR45687AQUAPORIN OR AQUAGLYCEROPORIN RELATEDcoord: 2..281
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 102..110

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy8G154380.1Chy8G154380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0006833 water transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015250 water channel activity
molecular_function GO:0015267 channel activity