Chy8G152270 (gene) Cucumber (hystrix) v1

Overview
NameChy8G152270
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionPyruvate decarboxylase
LocationchrH08: 14056572 .. 14059882 (-)
RNA-Seq ExpressionChy8G152270
SyntenyChy8G152270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAATCACGATGTGGATAAATAGTTTAGGCATCCGTCAACTTTTACCAATAAATAAGCTTCAAAATGGCTATCATTTCGGAACTCAAACCAGGCCAATGGTAGCCTATAGCCCAAGGATTGAGTGTGTAACCAATGGCTCATTAGCTTCCGATAAAATTATTAGCAGCAAGAATAATGGCTCCCCATTGGTCATAGTCCCACCGCCCACCACTGCACCATCGACACTTGGCCATTACTTAGCCCGTCGACTTGTTGAAATCGGTGTGTCTGATATCTTTTCAGTCCCAGGAGACTCTAATCTGGTTTTGTTTGATTACTTTGTGGCAGAAAAAGGGTTGAACCTTGTGGGGTGTTGCAACGAGCTTAATGCTGGTTATGCAGCCGATGGTTATGCTAGGAGCCGTGGTGTTGGAGCTTGTGCTGTGACATTCACTGTTGGTAGCCTTAGTCTTATCAATGCTATTGCAGGTGCTTACTCTGAGGATCTTCCTGTGATTTGTATTGTGGGCGGACCAAACTCAAATGATTATGGAAGTAAGAAGATTCTTCATCATACAATTGGGTTGCCGGATTTCAGCCAAGAACTTCGATGCTTTCAAAATGTCACTTGTCATCAGGTAGCTAGCATTTTAAATTAAAAATATTATTCGTTTACTGAGTACCACTTGGATGCTAGGGCAAGCAATAAAAATGATTTTGGAAGCATCTAAGTTTTTCACATATTGACAATAAAAAGAAGTGACTAAATAACTTTTCTTTTTTTTCCTTTCTTTCTTGTCGTTGTGTATAGGCCATTATTGACAGTTTGGAAGATGCACAGTGGCAAATAGATAAAGCAATTTGCAAGTGTTTGGAAGAAAGCAAACCAGTTTACATTAGCATTTGTTGCAATTTGGTGGCTATTCCTCATCCTTCTTTCTCTGCCCAACCACTCATCCCCCTGTCACTTTCTCCCAAGTACATATTTTTCATCCCAACAAATATTCCAATTTAATTTAAAGGACATAGTAATTATCAAAATAATGATCAGAGATTTCTTCCCTCAACAAGCAAGCAAATTTAACTTAATGTAACCTAATGGTCACGTCTATTGCTATCTTCATTTCTCTTCATCTACATTAATAAATTTGGTAAAAATACAACAAAGTGTAAGATAAATGAAGATGACCACAAGACATATCACCCTTGCATTAGGAGGCTATAACATTCACGATTATTCCTAACTAAAAAAAGGGTAGAAGACAATATATTAGATTTCATTTGTTTGTGAAGAGTAGCTCATGACTTGGTATGGTTCATTGATTAAAAAAAAACTCAAAATCAAACTCAATTTTTTTAAAAAAGTTTAAAAATATAACCTATCATGATTAACGGATAATAATCTTCTATCCTATTGCTAATATACCTATCATACAATTAGATAAGATCTAAATCTTGCTACATTCAAATTTTTATTTGCATTGTTTATATCTACAAATGCTTAGATATAATTACTTTATTTGCAACTGTGTCAATCTAAATATACTCTTCAAACATCAAATTAAAAAAATAAGAAAGTTAATCCATGATTAATTGTTGCTTAACGATATTACAGGCAAAGTAATCAAATGGGTTTAGAGATGGCAGTGGAAAAAGCAGCTGACCTTCTAAATACAGCCATCAAGCCCGTAATGATCGGCGGAAAGAAGCTACGACCCGCCAAAGCCGAAGCAGCATTCCTAAAGCTAGCCGACTCTTGTGGCTACGCCGTCGCGGTTACACCATCAGCAAAAGGCATGTTCCCGGAGAACCACCCACATTTCATCGGAACTTACTGGGGAACAGTGAGCACCGCCTTCTGCGGGGAGACGGTGGAAATAGCGGACGCCTCAATTTTCGTGGGAGCCACTTTAGACGAACTGGAAACAGTCGGCTACTCACTTACCTACAAAAAGAACCAAGCCATAATCGTAAAACCAGATAGCGTTGTGTTTCCAAACGGGGAGAGTTATGGGGCGGTTCAAATGAAGGATTTTCTTTGGGCTTTGGGTAAACGGTTGAAGCCCAATTTGAGGGCGTATGAGAATTATCGCCGGATTTACATAGCGGAGTCGAGTCCACCGGAATCGGAGGCGGGGGAGGAATTGCGAGTAAACGTTCTTTTCAAACATATTCAGAAAATGTTGAGCAGCAACATGACTGTGATAGCGGAGACTGGTGACTCTTGGTTTCATTCTCAAAAACTTAAACTACCAAAATCATGCGGGTATTTTTCTTTTCTTATCCAATTTAAATCTTTGCTTACCAAAGCTTTGATTATATTATTTGATCTAAAATATCATTTTCATCCCTTGCTTTCAAGGGATTTCATCTCCATGCTATTTTATTTATTTATTTATTTATTTTTGTTATGCATAGAATTTTAGTTAATTTCATTTTTGTCTCTAAACAATTCATATAGTTACCAATTTTCTTTTTTAAGAAATAACTATTTTGATCTTTTAAGTTAATCAATTTTAACGAATTCATTAGATACGACTGTGATATATTTTAAAGATGGAAAAGTAACAAATGAAGGAGCAAATTAAATGAGAGTGATGAAATGTGTAGGTATGAGGTACAATTGTTGTATGCATCAATTGGATGGTCTTTGGGAGCAACTTTAGGATATGCACAAGCTGCTCCACACAAGCGTTTACTTCTTTGTATTGGTGATGGAAGTTTTCAGGTCAACTTATATATCATTAAATACATTTCTTCTATATATATTTGATTTATTATTATTGTTTATATGCTTATTCTAAAGTTCAAATTTAGATACTTTCACTTGGTTTTTAACTTTATATTAAAAATATATATAAAATGATTATATCGTGACAGATGGCTCCACAAGATGTGTCTACAATGTTAAAATTGGAACAAAAAAATATTATATTTTTGATAAATAATGGGGGATACACAATTGAAGTAGAAATTCACGATGGTCCGTACAATATTATCAATAATTGGGATTACACTGCATTTGTTGACGCTGTAAACAATCATCAAAGCAATTGTTGGACTACAAAGGTGAATTCTCCTAGTACTTTTATTTCTCATGTACACTTTCTATAAATGGTTATTTTAAAATAAAATGTTGTTTGCACGTTAGGTTCATACGGAGGAAGAGTTGGTGAATGCAATTAAGATAGCAATGAAAGATAGAAATGATTGTTTGTGTTTCATAGAGGTCATTGCTCATAGAGATGATACAAGTAAAGAGTTACTTGAGTTTGGAAGCAGAATTGCAGCTATGGGTAGTCATCCTCCAAAGCTTTTGAAAAATTGA

mRNA sequence

ATGGGAATCACGATGTGGATAAATAGTTTAGGCATCCGTCAACTTTTACCAATAAATAAGCTTCAAAATGGCTATCATTTCGGAACTCAAACCAGGCCAATGGTAGCCTATAGCCCAAGGATTGAGTGTGTAACCAATGGCTCATTAGCTTCCGATAAAATTATTAGCAGCAAGAATAATGGCTCCCCATTGGTCATAGTCCCACCGCCCACCACTGCACCATCGACACTTGGCCATTACTTAGCCCGTCGACTTGTTGAAATCGGTGTGTCTGATATCTTTTCAGTCCCAGGAGACTCTAATCTGGTTTTGTTTGATTACTTTGTGGCAGAAAAAGGGTTGAACCTTGTGGGGTGTTGCAACGAGCTTAATGCTGGTTATGCAGCCGATGGTTATGCTAGGAGCCGTGGTGTTGGAGCTTGTGCTGTGACATTCACTGTTGGTAGCCTTAGTCTTATCAATGCTATTGCAGGTGCTTACTCTGAGGATCTTCCTGTGATTTGTATTGTGGGCGGACCAAACTCAAATGATTATGGAAGTAAGAAGATTCTTCATCATACAATTGGGTTGCCGGATTTCAGCCAAGAACTTCGATGCTTTCAAAATGTCACTTGTCATCAGGCCATTATTGACAGTTTGGAAGATGCACAGTGGCAAATAGATAAAGCAATTTGCAAGTGTTTGGAAGAAAGCAAACCAGTTTACATTAGCATTTGTTGCAATTTGGTGGCTATTCCTCATCCTTCTTTCTCTGCCCAACCACTCATCCCCCTGTCACTTTCTCCCAAGCAAAGTAATCAAATGGGTTTAGAGATGGCAGTGGAAAAAGCAGCTGACCTTCTAAATACAGCCATCAAGCCCGTAATGATCGGCGGAAAGAAGCTACGACCCGCCAAAGCCGAAGCAGCATTCCTAAAGCTAGCCGACTCTTGTGGCTACGCCGTCGCGGTTACACCATCAGCAAAAGGCATGTTCCCGGAGAACCACCCACATTTCATCGGAACTTACTGGGGAACAGTGAGCACCGCCTTCTGCGGGGAGACGGTGGAAATAGCGGACGCCTCAATTTTCGTGGGAGCCACTTTAGACGAACTGGAAACAGTCGGCTACTCACTTACCTACAAAAAGAACCAAGCCATAATCGTAAAACCAGATAGCGTTGTGTTTCCAAACGGGGAGAGTTATGGGGCGGTTCAAATGAAGGATTTTCTTTGGGCTTTGGGTAAACGGTTGAAGCCCAATTTGAGGGCGTATGAGAATTATCGCCGGATTTACATAGCGGAGTCGAGTCCACCGGAATCGGAGGCGGGGGAGGAATTGCGAGTAAACGTTCTTTTCAAACATATTCAGAAAATGTTGAGCAGCAACATGACTGTGATAGCGGAGACTGGTGACTCTTGGTTTCATTCTCAAAAACTTAAACTACCAAAATCATGCGGGTATGAGGTACAATTGTTGTATGCATCAATTGGATGGTCTTTGGGAGCAACTTTAGGATATGCACAAGCTGCTCCACACAAGCGTTTACTTCTTTGTATTGGTGATGGAAGTTTTCAGATGGCTCCACAAGATGTGTCTACAATGTTAAAATTGGAACAAAAAAATATTATATTTTTGATAAATAATGGGGGATACACAATTGAAGTAGAAATTCACGATGGTCCGTACAATATTATCAATAATTGGGATTACACTGCATTTGTTGACGCTGTAAACAATCATCAAAGCAATTGTTGGACTACAAAGGTTCATACGGAGGAAGAGTTGGTGAATGCAATTAAGATAGCAATGAAAGATAGAAATGATTGTTTGTGTTTCATAGAGGTCATTGCTCATAGAGATGATACAAGTAAAGAGTTACTTGAGTTTGGAAGCAGAATTGCAGCTATGGGTAGTCATCCTCCAAAGCTTTTGAAAAATTGA

Coding sequence (CDS)

ATGGGAATCACGATGTGGATAAATAGTTTAGGCATCCGTCAACTTTTACCAATAAATAAGCTTCAAAATGGCTATCATTTCGGAACTCAAACCAGGCCAATGGTAGCCTATAGCCCAAGGATTGAGTGTGTAACCAATGGCTCATTAGCTTCCGATAAAATTATTAGCAGCAAGAATAATGGCTCCCCATTGGTCATAGTCCCACCGCCCACCACTGCACCATCGACACTTGGCCATTACTTAGCCCGTCGACTTGTTGAAATCGGTGTGTCTGATATCTTTTCAGTCCCAGGAGACTCTAATCTGGTTTTGTTTGATTACTTTGTGGCAGAAAAAGGGTTGAACCTTGTGGGGTGTTGCAACGAGCTTAATGCTGGTTATGCAGCCGATGGTTATGCTAGGAGCCGTGGTGTTGGAGCTTGTGCTGTGACATTCACTGTTGGTAGCCTTAGTCTTATCAATGCTATTGCAGGTGCTTACTCTGAGGATCTTCCTGTGATTTGTATTGTGGGCGGACCAAACTCAAATGATTATGGAAGTAAGAAGATTCTTCATCATACAATTGGGTTGCCGGATTTCAGCCAAGAACTTCGATGCTTTCAAAATGTCACTTGTCATCAGGCCATTATTGACAGTTTGGAAGATGCACAGTGGCAAATAGATAAAGCAATTTGCAAGTGTTTGGAAGAAAGCAAACCAGTTTACATTAGCATTTGTTGCAATTTGGTGGCTATTCCTCATCCTTCTTTCTCTGCCCAACCACTCATCCCCCTGTCACTTTCTCCCAAGCAAAGTAATCAAATGGGTTTAGAGATGGCAGTGGAAAAAGCAGCTGACCTTCTAAATACAGCCATCAAGCCCGTAATGATCGGCGGAAAGAAGCTACGACCCGCCAAAGCCGAAGCAGCATTCCTAAAGCTAGCCGACTCTTGTGGCTACGCCGTCGCGGTTACACCATCAGCAAAAGGCATGTTCCCGGAGAACCACCCACATTTCATCGGAACTTACTGGGGAACAGTGAGCACCGCCTTCTGCGGGGAGACGGTGGAAATAGCGGACGCCTCAATTTTCGTGGGAGCCACTTTAGACGAACTGGAAACAGTCGGCTACTCACTTACCTACAAAAAGAACCAAGCCATAATCGTAAAACCAGATAGCGTTGTGTTTCCAAACGGGGAGAGTTATGGGGCGGTTCAAATGAAGGATTTTCTTTGGGCTTTGGGTAAACGGTTGAAGCCCAATTTGAGGGCGTATGAGAATTATCGCCGGATTTACATAGCGGAGTCGAGTCCACCGGAATCGGAGGCGGGGGAGGAATTGCGAGTAAACGTTCTTTTCAAACATATTCAGAAAATGTTGAGCAGCAACATGACTGTGATAGCGGAGACTGGTGACTCTTGGTTTCATTCTCAAAAACTTAAACTACCAAAATCATGCGGGTATGAGGTACAATTGTTGTATGCATCAATTGGATGGTCTTTGGGAGCAACTTTAGGATATGCACAAGCTGCTCCACACAAGCGTTTACTTCTTTGTATTGGTGATGGAAGTTTTCAGATGGCTCCACAAGATGTGTCTACAATGTTAAAATTGGAACAAAAAAATATTATATTTTTGATAAATAATGGGGGATACACAATTGAAGTAGAAATTCACGATGGTCCGTACAATATTATCAATAATTGGGATTACACTGCATTTGTTGACGCTGTAAACAATCATCAAAGCAATTGTTGGACTACAAAGGTTCATACGGAGGAAGAGTTGGTGAATGCAATTAAGATAGCAATGAAAGATAGAAATGATTGTTTGTGTTTCATAGAGGTCATTGCTCATAGAGATGATACAAGTAAAGAGTTACTTGAGTTTGGAAGCAGAATTGCAGCTATGGGTAGTCATCCTCCAAAGCTTTTGAAAAATTGA

Protein sequence

MGITMWINSLGIRQLLPINKLQNGYHFGTQTRPMVAYSPRIECVTNGSLASDKIISSKNNGSPLVIVPPPTTAPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCCNELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICCNLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKAEAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGATLDELETVGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYENYRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCGYEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLINNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDRNDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPPKLLKN*
Homology
BLAST of Chy8G152270 vs. ExPASy Swiss-Prot
Match: A2Y5L9 (Pyruvate decarboxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 8.0e-229
Identity = 379/575 (65.91%), Postives = 457/575 (79.48%), Query Frame = 0

Query: 62  SPLVIVPPPTTAP-STLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 121
           S  VI PP T+A  +TLG +LARRLV+IG +D+F+VPGD NL L DY +AE GL L+GCC
Sbjct: 29  SNAVIHPPVTSAAGATLGRHLARRLVQIGATDVFAVPGDFNLTLLDYLIAEPGLKLIGCC 88

Query: 122 NELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS 181
           NELNAGYAADGYAR+RGVGACAVTFTVG LS++NAIAGAYSE+LPVICIVGGPNSNDYG+
Sbjct: 89  NELNAGYAADGYARARGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDYGT 148

Query: 182 KKILHHTIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICC 241
            +ILHHTIGLPDFSQELRCFQ +TC+QA+I++L+DA  QID AI   L ESKPVYIS+ C
Sbjct: 149 NRILHHTIGLPDFSQELRCFQTITCYQAVINNLDDAHEQIDTAIATALRESKPVYISVGC 208

Query: 242 NLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 301
           NL  + HP+FS +P +PL +SP+ SN+  LE AVE AAD LN A+KPVM+GG K+R AKA
Sbjct: 209 NLAGLSHPTFSREP-VPLFISPRLSNKANLEYAVEAAADFLNKAVKPVMVGGPKIRVAKA 268

Query: 302 EAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 361
           + AF  +A+S GY +AV PSAKG+ PE+HP FIGTYWG VST FC E VE ADA +F G 
Sbjct: 269 KKAFAGIAESSGYPIAVMPSAKGLVPEHHPRFIGTYWGAVSTTFCAEIVESADAYLFAGP 328

Query: 362 TLDELETVGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYEN 421
             ++  +VGYSL  K+ +A+IV+PD VV  NG ++G + M +FL AL KRL  N  AY+N
Sbjct: 329 IFNDYSSVGYSLLLKREKAVIVQPDRVVVGNGPAFGCILMTEFLDALAKRLDRNTTAYDN 388

Query: 422 YRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 481
           YRRI+I +  PP  +  E LRVN+LFKHI++MLS +  VIAETGDSWF+ QKL+LP+ CG
Sbjct: 389 YRRIFIPDREPPNGQPDEPLRVNILFKHIKEMLSGDTAVIAETGDSWFNCQKLRLPEGCG 448

Query: 482 YEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLI 541
           YE Q+ Y SIGWS+GATLGYAQAA  KR++ CIGDGSFQM  QDVSTML+  QK+IIFLI
Sbjct: 449 YEFQMQYGSIGWSVGATLGYAQAAKDKRVISCIGDGSFQMTAQDVSTMLRCGQKSIIFLI 508

Query: 542 NNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDR 601
           NNGGYTIEVEIHDGPYN+I NWDYT  +DA++N   NCWT KV TEEEL+ AI  A   +
Sbjct: 509 NNGGYTIEVEIHDGPYNVIKNWDYTGLIDAIHNSDGNCWTKKVRTEEELIEAIATATGAK 568

Query: 602 NDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPP 636
            DCLCFIE+I H+DDTSKELLE+GSR++A  S PP
Sbjct: 569 KDCLCFIEIIVHKDDTSKELLEWGSRVSAANSRPP 602

BLAST of Chy8G152270 vs. ExPASy Swiss-Prot
Match: A2XFI3 (Pyruvate decarboxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=PDC2 PE=2 SV=2)

HSP 1 Score: 793.5 bits (2048), Expect = 1.8e-228
Identity = 375/567 (66.14%), Postives = 451/567 (79.54%), Query Frame = 0

Query: 69  PPTTAPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCCNELNAGYA 128
           P +   ++LG +LARRLV++GVSD+F+VPGD NL L D+ +AE GL LVGCCNELNAGYA
Sbjct: 38  PVSAGEASLGRHLARRLVQVGVSDVFAVPGDFNLTLLDHLIAEPGLRLVGCCNELNAGYA 97

Query: 129 ADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTI 188
           ADGYAR+RGVGACAVTFTVG LS++NAIAGAYSE+LPVICI GGPNSNDYG+ +ILHHTI
Sbjct: 98  ADGYARARGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIAGGPNSNDYGTNRILHHTI 157

Query: 189 GLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICCNLVAIPHP 248
           GLPDFSQELRCFQ VTCHQA++ +LEDA  QID AI   L ESKPVY+SI CNL  +PHP
Sbjct: 158 GLPDFSQELRCFQTVTCHQAVVTNLEDAHEQIDTAIATALRESKPVYLSISCNLPGLPHP 217

Query: 249 SFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKAEAAFLKLA 308
           +FS  P +P  L+P+ SN+MGLE AVE   + LN A+KPV++GG KLR AKA  AF+ L 
Sbjct: 218 TFSRDP-VPFFLAPRLSNKMGLEAAVEATVEFLNKAVKPVLVGGPKLRVAKAGKAFVDLV 277

Query: 309 DSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGATLDELETV 368
           D+ GYA AV PSAKG+ PE HPHFIGTYWG VSTAFC E VE ADA +F G   ++  +V
Sbjct: 278 DASGYAYAVMPSAKGLVPETHPHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSV 337

Query: 369 GYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYENYRRIYIAE 428
           GYS   KK++AIIV+P+ V+  NG ++G V MK+FL  L KR+  N  AYENY+RI++ E
Sbjct: 338 GYSFLLKKDKAIIVQPERVIVGNGPAFGCVMMKEFLSELAKRVNKNTTAYENYKRIFVPE 397

Query: 429 SSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCGYEVQLLYA 488
             P ESE  E LRVNVLFKH+QKML+S+  VIAETGDSWF+ QKLKLP+ CGYE Q+ Y 
Sbjct: 398 GQPLESEPNEPLRVNVLFKHVQKMLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQYG 457

Query: 489 SIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLINNGGYTIE 548
           SIGWS+GA LGYAQ A  KR++ CIGDGSFQ+  QDVSTM++  Q +IIFLINNGGYTIE
Sbjct: 458 SIGWSVGALLGYAQGAKDKRVIACIGDGSFQVTAQDVSTMIRCAQNSIIFLINNGGYTIE 517

Query: 549 VEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDRNDCLCFIE 608
           VEIHDGPYN+I NW+YT  VDA++N +  CWT+KV  EEEL  AI +A+ ++ DCLCFIE
Sbjct: 518 VEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTSKVKCEEELTEAIGMALGEKKDCLCFIE 577

Query: 609 VIAHRDDTSKELLEFGSRIAAMGSHPP 636
           VIAH+DDTSKELLE+GSR++A  S PP
Sbjct: 578 VIAHKDDTSKELLEWGSRVSAANSRPP 603

BLAST of Chy8G152270 vs. ExPASy Swiss-Prot
Match: Q0DHF6 (Pyruvate decarboxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 2.3e-228
Identity = 379/575 (65.91%), Postives = 456/575 (79.30%), Query Frame = 0

Query: 62  SPLVIVPPPTTAP-STLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 121
           S  VI PP T+A  +TLG +LARRLV+IG +D+F+VPGD NL L DY +AE GL L+GCC
Sbjct: 29  SNAVINPPVTSAAGATLGRHLARRLVQIGATDVFAVPGDFNLTLLDYLIAEPGLKLIGCC 88

Query: 122 NELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS 181
           NELNAGYAADGYAR+RGVGACAVTFTVG LS++NAIAGAYSE+LPVICIVGGPNSNDYG+
Sbjct: 89  NELNAGYAADGYARARGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDYGT 148

Query: 182 KKILHHTIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICC 241
            +ILHHTIGLPDFSQELRCFQ +TC+QA+I++L+DA  QID AI   L ESKPVYIS+ C
Sbjct: 149 NRILHHTIGLPDFSQELRCFQTITCYQAVINNLDDAHEQIDTAIATALRESKPVYISVGC 208

Query: 242 NLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 301
           NL  + HP+FS +P +PL +SP+ SN+  LE AVE AAD LN A+KPVM+GG K+R AKA
Sbjct: 209 NLAGLSHPTFSREP-VPLFISPRLSNKANLEYAVEAAADFLNKAVKPVMVGGPKIRVAKA 268

Query: 302 EAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 361
           + AF  +A+S GY  AV PSAKG+ PE+HP FIGTYWG VST FC E VE ADA +F G 
Sbjct: 269 KKAFAGIAESSGYPFAVMPSAKGLVPEHHPRFIGTYWGAVSTTFCAEIVESADAYLFAGP 328

Query: 362 TLDELETVGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYEN 421
             ++  +VGYSL  K+ +A+IV+PD VV  NG ++G + M +FL AL KRL  N  AY+N
Sbjct: 329 IFNDYSSVGYSLLLKREKAVIVQPDRVVVGNGPAFGCILMTEFLDALAKRLDRNTTAYDN 388

Query: 422 YRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 481
           YRRI+I +  PP  +  E LRVN+LFKHI++MLS +  VIAETGDSWF+ QKL+LP+ CG
Sbjct: 389 YRRIFIPDREPPNGQPDEPLRVNILFKHIKEMLSGDTAVIAETGDSWFNCQKLRLPEGCG 448

Query: 482 YEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLI 541
           YE Q+ Y SIGWS+GATLGYAQAA  KR++ CIGDGSFQM  QDVSTML+  QK+IIFLI
Sbjct: 449 YEFQMQYGSIGWSVGATLGYAQAAKDKRVISCIGDGSFQMTAQDVSTMLRCGQKSIIFLI 508

Query: 542 NNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDR 601
           NNGGYTIEVEIHDGPYN+I NWDYT  +DA++N   NCWT KV TEEEL+ AI  A   +
Sbjct: 509 NNGGYTIEVEIHDGPYNVIKNWDYTGLIDAIHNSDGNCWTKKVRTEEELIEAIATATGAK 568

Query: 602 NDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPP 636
            DCLCFIE+I H+DDTSKELLE+GSR++A  S PP
Sbjct: 569 KDCLCFIEIIVHKDDTSKELLEWGSRVSAANSRPP 602

BLAST of Chy8G152270 vs. ExPASy Swiss-Prot
Match: Q9M040 (Pyruvate decarboxylase 4 OS=Arabidopsis thaliana OX=3702 GN=PDC4 PE=2 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 2.3e-228
Identity = 382/568 (67.25%), Postives = 447/568 (78.70%), Query Frame = 0

Query: 68  PPPTTAPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCCNELNAGY 127
           P  TT+ STLG +L+RRLV+ GV+D+FSVPGD NL L D+ +AE  LN +GCCNELNAGY
Sbjct: 34  PITTTSESTLGRHLSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEPELNNIGCCNELNAGY 93

Query: 128 AADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT 187
           AADGYARSRGVGAC VTFTVG LS++NAIAGAYSE+LPVICIVGGPNSND+G+ +ILHHT
Sbjct: 94  AADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHT 153

Query: 188 IGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICCNLVAIPH 247
           IGLPDFSQELRCFQ VTC+QA++++LEDA  QIDKAI   L+ESKPVYISI CNL A PH
Sbjct: 154 IGLPDFSQELRCFQTVTCYQAVVNNLEDAHEQIDKAIATALKESKPVYISISCNLAATPH 213

Query: 248 PSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKAEAAFLKL 307
           P+F+  P +P  L+P+ SN MGLE AVE   + LN A+KPVM+GG KLR AKA  AFL+L
Sbjct: 214 PTFARDP-VPFDLTPRMSNTMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKASEAFLEL 273

Query: 308 ADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGATLDELET 367
           AD+ GY +AV PS KG+ PENHPHFIGTYWG VST FC E VE ADA IF G   ++  +
Sbjct: 274 ADASGYPLAVMPSTKGLVPENHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSS 333

Query: 368 VGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYENYRRIYIA 427
           VGYSL  KK +AIIV PD VV  NG ++G V M DF   L KR+K N  AYENY RI++ 
Sbjct: 334 VGYSLLLKKEKAIIVHPDRVVVANGPTFGCVLMSDFFRELAKRVKRNETAYENYERIFVP 393

Query: 428 ESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCGYEVQLLY 487
           E  P + + GE LRVN +F+HIQKMLSS   VIAETGDSWF+ QKLKLPK CGYE Q+ Y
Sbjct: 394 EGKPLKCKPGEPLRVNAMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQY 453

Query: 488 ASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLINNGGYTI 547
            SIGWS+GATLGYAQA P KR+L  IGDGSFQ+  QD+STM++  QK IIFLINNGGYTI
Sbjct: 454 GSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDISTMIRNGQKAIIFLINNGGYTI 513

Query: 548 EVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDRNDCLCFI 607
           EVEIHDGPYN+I NW+YT  VDA++N +  CWTTKV  EEELV AIK A  ++ D LCFI
Sbjct: 514 EVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAIKTATTEKKDSLCFI 573

Query: 608 EVIAHRDDTSKELLEFGSRIAAMGSHPP 636
           EVI H+DDTSKELLE+GSR++A    PP
Sbjct: 574 EVIVHKDDTSKELLEWGSRVSAANGRPP 600

BLAST of Chy8G152270 vs. ExPASy Swiss-Prot
Match: O82647 (Pyruvate decarboxylase 1 OS=Arabidopsis thaliana OX=3702 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 2.6e-227
Identity = 377/561 (67.20%), Postives = 449/561 (80.04%), Query Frame = 0

Query: 75  STLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCCNELNAGYAADGYAR 134
           +TLG +LARRLV+ GV+D+FSVPGD NL L D+ +AE  LNL+GCCNELNAGYAADGYAR
Sbjct: 45  ATLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYAR 104

Query: 135 SRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLPDFS 194
           SRGVGAC VTFTVG LS++NAIAGAYSE+LP+ICIVGGPNSNDYG+ +ILHHTIGLPDFS
Sbjct: 105 SRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFS 164

Query: 195 QELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICCNLVAIPHPSFSAQP 254
           QELRCFQ VTC+QA++++L+DA  QIDKAI   L+ESKPVYIS+ CNL AIPH +FS  P
Sbjct: 165 QELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALKESKPVYISVSCNLAAIPHHTFSRDP 224

Query: 255 LIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKAEAAFLKLADSCGYA 314
            +P SL+P+ SN+MGLE AVE   + LN A+KPVM+GG KLR AKA  AF++LAD+ GYA
Sbjct: 225 -VPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKACDAFVELADASGYA 284

Query: 315 VAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGATLDELETVGYSLTY 374
           +A+ PSAKG  PE+HPHFIGTYWG VST FC E VE ADA IF G   ++  +VGYSL  
Sbjct: 285 LAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLL 344

Query: 375 KKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYENYRRIYIAESSPPES 434
           KK +AI+V+PD +   NG ++G + M DF   L KR+K N  AYENY RI++ E  P + 
Sbjct: 345 KKEKAIVVQPDRITVANGPTFGCILMSDFFRELSKRVKRNETAYENYHRIFVPEGKPLKC 404

Query: 435 EAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCGYEVQLLYASIGWSL 494
           E+ E LRVN +F+HIQKMLSS   VIAETGDSWF+ QKLKLPK CGYE Q+ Y SIGWS+
Sbjct: 405 ESREPLRVNTMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSV 464

Query: 495 GATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLINNGGYTIEVEIHDG 554
           GATLGYAQA+P KR+L  IGDGSFQ+  QD+STML+  QK IIFLINNGGYTIEVEIHDG
Sbjct: 465 GATLGYAQASPEKRVLAFIGDGSFQVTVQDISTMLRNGQKTIIFLINNGGYTIEVEIHDG 524

Query: 555 PYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDRNDCLCFIEVIAHRD 614
           PYN+I NW+YT  VDA++N + NCWT KV  EEELV AI  A  ++ DCLCFIEVI H+D
Sbjct: 525 PYNVIKNWNYTGLVDAIHNGEGNCWTAKVRYEEELVEAITTATTEKKDCLCFIEVILHKD 584

Query: 615 DTSKELLEFGSRIAAMGSHPP 636
           DTSKELLE+GSR++A  S PP
Sbjct: 585 DTSKELLEWGSRVSAANSRPP 604

BLAST of Chy8G152270 vs. ExPASy TrEMBL
Match: A0A0A0L1C6 (Pyruvate decarboxylase OS=Cucumis sativus OX=3659 GN=Csa_4G312250 PE=3 SV=1)

HSP 1 Score: 1265.8 bits (3274), Expect = 0.0e+00
Identity = 623/640 (97.34%), Postives = 630/640 (98.44%), Query Frame = 0

Query: 1   MGITMWINSLGIRQLLPINKLQNGYHFGTQTRPMVAYSPRIECVTNGSLASDKIISSKNN 60
           MGITMWINSLG+ QLL +NKL+NGYHF TQTRPMVAYSPRIE VTN SLASDKIISSKNN
Sbjct: 1   MGITMWINSLGVGQLLRMNKLENGYHFQTQTRPMVAYSPRIERVTNASLASDKIISSKNN 60

Query: 61  GSPLVIVPPPTTAPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120
           GSPLVIVPPPTTAPSTLGHYLA RLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC
Sbjct: 61  GSPLVIVPPPTTAPSTLGHYLASRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120

Query: 121 NELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS 180
           NELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS
Sbjct: 121 NELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS 180

Query: 181 KKILHHTIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICC 240
           KKILHHTIGLPDFSQELRCFQNVTC+QAIIDSLEDAQWQIDKAICKCLEESKPVYISICC
Sbjct: 181 KKILHHTIGLPDFSQELRCFQNVTCYQAIIDSLEDAQWQIDKAICKCLEESKPVYISICC 240

Query: 241 NLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300
           NLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA
Sbjct: 241 NLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300

Query: 301 EAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360
           EAAFL+LADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA
Sbjct: 301 EAAFLELADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360

Query: 361 TLDELETVGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYEN 420
            LDELETVGYSL YKKN+AIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPN RAYEN
Sbjct: 361 NLDELETVGYSLAYKKNKAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNSRAYEN 420

Query: 421 YRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 480
           YRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG
Sbjct: 421 YRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 480

Query: 481 YEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLI 540
           YEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKL QKNIIFLI
Sbjct: 481 YEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLGQKNIIFLI 540

Query: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDR 600
           NNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAI+IAMKDR
Sbjct: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIEIAMKDR 600

Query: 601 NDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPPKLLKN 641
           NDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPPKLLKN
Sbjct: 601 NDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPPKLLKN 640

BLAST of Chy8G152270 vs. ExPASy TrEMBL
Match: A0A5D3E1K9 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold669G00080 PE=3 SV=1)

HSP 1 Score: 1214.5 bits (3141), Expect = 0.0e+00
Identity = 596/640 (93.12%), Postives = 617/640 (96.41%), Query Frame = 0

Query: 1   MGITMWINSLGIRQLLPINKLQNGYHFGTQTRPMVAYSPRIECVTNGSLASDKIISSKNN 60
           MGITMWINSLGI Q+LP+NKL    HF TQTRPM AY+PRIE VTNGSLASDK+ISS NN
Sbjct: 1   MGITMWINSLGISQILPMNKLN---HFQTQTRPMAAYNPRIEHVTNGSLASDKLISSNNN 60

Query: 61  GSPLVIVPPPTTAPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120
           GSPLVIVPPP T PSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC
Sbjct: 61  GSPLVIVPPPVTVPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120

Query: 121 NELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS 180
           NELNAGYAADGYAR+RGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS
Sbjct: 121 NELNAGYAADGYARNRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS 180

Query: 181 KKILHHTIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICC 240
           KKILHHTIGLPDFSQELRCFQNVTC+QA+ID+LEDAQWQIDKAICKCLEESKPVYISICC
Sbjct: 181 KKILHHTIGLPDFSQELRCFQNVTCYQAVIDNLEDAQWQIDKAICKCLEESKPVYISICC 240

Query: 241 NLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300
           NLVAIPHPSFSA+PLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA
Sbjct: 241 NLVAIPHPSFSAKPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300

Query: 301 EAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360
           EAAFL+LA++CGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA
Sbjct: 301 EAAFLELANTCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360

Query: 361 TLDELETVGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYEN 420
           TLDELETVGYSLTYKKN+AI+VKPD VVFPNG SYG VQ+KDFL ALGKRLKPNLRAYEN
Sbjct: 361 TLDELETVGYSLTYKKNKAIMVKPDGVVFPNGASYGGVQIKDFLRALGKRLKPNLRAYEN 420

Query: 421 YRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 480
           YRRIYIAES PPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG
Sbjct: 421 YRRIYIAESGPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 480

Query: 481 YEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLI 540
           YE+QLLYASIGWSLGATLGYAQ+AP KRL+LCIGDGSFQMAPQDVSTMLKL Q NIIFLI
Sbjct: 481 YEIQLLYASIGWSLGATLGYAQSAPQKRLVLCIGDGSFQMAPQDVSTMLKLGQNNIIFLI 540

Query: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDR 600
           NNGGYTIEVEIHDGPYNIINNWDYTAFV+AVNNHQ NCWTTKVHTEEELV AI+IAM+DR
Sbjct: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVEAVNNHQGNCWTTKVHTEEELVRAIEIAMEDR 600

Query: 601 NDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPPKLLKN 641
           ND LCFIEVI HRDDTSKELLEFGSRIAAMGSHPPKLLKN
Sbjct: 601 NDYLCFIEVIVHRDDTSKELLEFGSRIAAMGSHPPKLLKN 637

BLAST of Chy8G152270 vs. ExPASy TrEMBL
Match: A0A1S3CKY9 (Pyruvate decarboxylase OS=Cucumis melo OX=3656 GN=LOC103502149 PE=3 SV=1)

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 595/640 (92.97%), Postives = 616/640 (96.25%), Query Frame = 0

Query: 1   MGITMWINSLGIRQLLPINKLQNGYHFGTQTRPMVAYSPRIECVTNGSLASDKIISSKNN 60
           MGITMWINSLGI Q+LP+NKL    HF TQTRPM AY+PRIE VTNGSLASDK+ISS NN
Sbjct: 1   MGITMWINSLGISQILPMNKLN---HFQTQTRPMAAYNPRIEHVTNGSLASDKLISSNNN 60

Query: 61  GSPLVIVPPPTTAPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120
           GSPLVIVPPP T PSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC
Sbjct: 61  GSPLVIVPPPVTVPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120

Query: 121 NELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS 180
           NELNAGYAADGYAR+RGVGACAVTFTVGSLSLI+AIAGAYSEDLPVICIVGGPNSNDYGS
Sbjct: 121 NELNAGYAADGYARNRGVGACAVTFTVGSLSLISAIAGAYSEDLPVICIVGGPNSNDYGS 180

Query: 181 KKILHHTIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICC 240
           KKILHHTIGLPDFSQELRCFQNVTC+QA+ID+LEDAQWQIDKAICKCLEESKPVYISICC
Sbjct: 181 KKILHHTIGLPDFSQELRCFQNVTCYQAVIDNLEDAQWQIDKAICKCLEESKPVYISICC 240

Query: 241 NLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300
           NLVAIPHPSFSA+PLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA
Sbjct: 241 NLVAIPHPSFSAKPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300

Query: 301 EAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360
           EAAFL+LA++CGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA
Sbjct: 301 EAAFLELANTCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360

Query: 361 TLDELETVGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYEN 420
           TLDELETVG SLTYKKN+AI+VKPD VVFPNG SYG VQMKDFL ALGKRLKPNLRAYEN
Sbjct: 361 TLDELETVGXSLTYKKNKAIMVKPDGVVFPNGASYGGVQMKDFLRALGKRLKPNLRAYEN 420

Query: 421 YRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 480
           YRRIYIAES PPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG
Sbjct: 421 YRRIYIAESGPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 480

Query: 481 YEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLI 540
           YE+QLLYASIGWSLGATLGYAQ+AP KRL+LCIGDGSFQMAPQDVSTMLKL Q NIIFLI
Sbjct: 481 YEIQLLYASIGWSLGATLGYAQSAPQKRLVLCIGDGSFQMAPQDVSTMLKLGQNNIIFLI 540

Query: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDR 600
           NNGGYTIEVEIHDGPYNIINNWDYTAFV+AVNNHQ NCWTTKVHTEEELV AI+IAM+DR
Sbjct: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVEAVNNHQGNCWTTKVHTEEELVRAIEIAMEDR 600

Query: 601 NDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPPKLLKN 641
           NDCLCF EVI HRDDTSKELLEFGSRIAAMGSHPPKLLKN
Sbjct: 601 NDCLCFTEVIVHRDDTSKELLEFGSRIAAMGSHPPKLLKN 637

BLAST of Chy8G152270 vs. ExPASy TrEMBL
Match: A0A5A7T2Y2 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold141G00360 PE=3 SV=1)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 554/640 (86.56%), Postives = 573/640 (89.53%), Query Frame = 0

Query: 1   MGITMWINSLGIRQLLPINKLQNGYHFGTQTRPMVAYSPRIECVTNGSLASDKIISSKNN 60
           MGITMWINSLGI Q+LP+NKL    HF TQTRPM AY+PRIE VTNGSLASDK+ISS NN
Sbjct: 1   MGITMWINSLGISQILPMNKLN---HFQTQTRPMAAYNPRIEHVTNGSLASDKLISSNNN 60

Query: 61  GSPLVIVPPPTTAPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120
           GSPLVIVPPP T PSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC
Sbjct: 61  GSPLVIVPPPVTVPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120

Query: 121 NELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS 180
           NELNAGYAADGYAR+RGVGACAVTFTVGSLSLI+AIAGAYSEDLPVICIVGGPNSNDYGS
Sbjct: 121 NELNAGYAADGYARNRGVGACAVTFTVGSLSLISAIAGAYSEDLPVICIVGGPNSNDYGS 180

Query: 181 KKILHHTIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICC 240
           KKILHHTIGLPDFSQELRCFQNVTC+QA+ID+LEDAQWQIDKAICKCLEESKPVYISICC
Sbjct: 181 KKILHHTIGLPDFSQELRCFQNVTCYQAVIDNLEDAQWQIDKAICKCLEESKPVYISICC 240

Query: 241 NLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300
           NLVAIPHPSFSA+PLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA
Sbjct: 241 NLVAIPHPSFSAKPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300

Query: 301 EAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360
           EAAFL+LA++CGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA
Sbjct: 301 EAAFLELANTCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360

Query: 361 TLDELETVGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYEN 420
           TLDELET                                  DFL ALGKRLKPNLRAYEN
Sbjct: 361 TLDELET----------------------------------DFLRALGKRLKPNLRAYEN 420

Query: 421 YRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 480
           YRRIYIAES PPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSW             
Sbjct: 421 YRRIYIAESGPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSW------------- 480

Query: 481 YEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLI 540
           YE+QLLYASIGWSLGATLGYAQ+AP KRL+LCIGDGSFQMAPQDVSTMLKL Q NIIFLI
Sbjct: 481 YEIQLLYASIGWSLGATLGYAQSAPQKRLVLCIGDGSFQMAPQDVSTMLKLGQNNIIFLI 540

Query: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDR 600
           NNGGYTIEVEIHDGPYNIINNWDYTAFV+AVNNHQ NCWTTKVHTEEELV AI+IAM+DR
Sbjct: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVEAVNNHQGNCWTTKVHTEEELVRAIEIAMEDR 590

Query: 601 NDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPPKLLKN 641
           NDCLCF EVI HRDDTSKELLEFGSRIAAMGSHPPKLLKN
Sbjct: 601 NDCLCFTEVIVHRDDTSKELLEFGSRIAAMGSHPPKLLKN 590

BLAST of Chy8G152270 vs. ExPASy TrEMBL
Match: A0A5D3E0N5 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold669G00060 PE=3 SV=1)

HSP 1 Score: 1024.6 bits (2648), Expect = 1.8e-295
Identity = 501/640 (78.28%), Postives = 557/640 (87.03%), Query Frame = 0

Query: 20  KLQNGYH----------FGTQTRPMV------AYSPRIECVTNGSLASDKI-----ISSK 79
           KL+NG+H          F T+T P V      ++SP ++ + NG  A+DK+     I++ 
Sbjct: 4   KLENGFHTETNQKVENGFRTETNPTVTTTIAASHSPTVKPLANGIAAADKLRSQSRINNH 63

Query: 80  NNGSPLVIVPPPTTAPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVG 139
           NNG+  V +P PTT PSTLGHYLARRLV+IG+SDIFSVPGDSNLV+FDYFVAEKGLNLVG
Sbjct: 64  NNGA--VTIPTPTTTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVG 123

Query: 140 CCNELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDY 199
           CCNELNAGYAADGYAR RGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDY
Sbjct: 124 CCNELNAGYAADGYARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDY 183

Query: 200 GSKKILHHTIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISI 259
           GSKKILHHTIGLPDF QELRCFQNVTC+QAIID+LEDAQW+ID AI KCLEE KP+YISI
Sbjct: 184 GSKKILHHTIGLPDFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISI 243

Query: 260 CCNLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPA 319
           CCNLVAIPHPSFSAQPLIPLSLSPKQSNQM LE AVEKAA LLNTAIKPVMIGGKKLRP 
Sbjct: 244 CCNLVAIPHPSFSAQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPT 303

Query: 320 KAEAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFV 379
           K++  F++LA++CGY VAVTPSAKGMFPENHPHFIG YWGTVS+A+CGETVEIADASIFV
Sbjct: 304 KSQEPFIELANACGYPVAVTPSAKGMFPENHPHFIGIYWGTVSSAYCGETVEIADASIFV 363

Query: 380 GATLDELETVGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAY 439
           GATLDELETV ++LTYKKN+AI+V+ D VVFPNG S+G + MKDFLWAL KRLKPN  AY
Sbjct: 364 GATLDELETVAFTLTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAY 423

Query: 440 ENYRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKS 499
           ENYRRIYIAES P ES  GE LRVN+LFKHIQKMLSSNM VI+E GDSWFH Q+LKLPKS
Sbjct: 424 ENYRRIYIAESGPLESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKS 483

Query: 500 CGYEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIF 559
           CGYEVQLLYASIGWSLGATLGYAQAAP +R++LCIGDGSFQM PQDVSTM+ L+Q NIIF
Sbjct: 484 CGYEVQLLYASIGWSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIF 543

Query: 560 LINNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMK 619
           LINNGGYTIEVEIHDGPYN+I NWDYTA V A++NH   CWTTKVHTEEEL+NAI+I ++
Sbjct: 544 LINNGGYTIEVEIHDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLE 603

Query: 620 DRNDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPPKLL 639
           +R D LCFIEVI HRDDTS+ELLEFGS+ AA+ S PP+ L
Sbjct: 604 ERKDYLCFIEVIVHRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of Chy8G152270 vs. NCBI nr
Match: XP_004148631.3 (pyruvate decarboxylase 1 [Cucumis sativus] >KGN54392.1 hypothetical protein Csa_018074 [Cucumis sativus])

HSP 1 Score: 1261 bits (3262), Expect = 0.0
Identity = 623/640 (97.34%), Postives = 630/640 (98.44%), Query Frame = 0

Query: 1   MGITMWINSLGIRQLLPINKLQNGYHFGTQTRPMVAYSPRIECVTNGSLASDKIISSKNN 60
           MGITMWINSLG+ QLL +NKL+NGYHF TQTRPMVAYSPRIE VTN SLASDKIISSKNN
Sbjct: 1   MGITMWINSLGVGQLLRMNKLENGYHFQTQTRPMVAYSPRIERVTNASLASDKIISSKNN 60

Query: 61  GSPLVIVPPPTTAPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120
           GSPLVIVPPPTTAPSTLGHYLA RLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC
Sbjct: 61  GSPLVIVPPPTTAPSTLGHYLASRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120

Query: 121 NELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS 180
           NELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS
Sbjct: 121 NELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS 180

Query: 181 KKILHHTIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICC 240
           KKILHHTIGLPDFSQELRCFQNVTC+QAIIDSLEDAQWQIDKAICKCLEESKPVYISICC
Sbjct: 181 KKILHHTIGLPDFSQELRCFQNVTCYQAIIDSLEDAQWQIDKAICKCLEESKPVYISICC 240

Query: 241 NLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300
           NLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA
Sbjct: 241 NLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300

Query: 301 EAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360
           EAAFL+LADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA
Sbjct: 301 EAAFLELADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360

Query: 361 TLDELETVGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYEN 420
            LDELETVGYSL YKKN+AIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPN RAYEN
Sbjct: 361 NLDELETVGYSLAYKKNKAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNSRAYEN 420

Query: 421 YRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 480
           YRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG
Sbjct: 421 YRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 480

Query: 481 YEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLI 540
           YEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKL QKNIIFLI
Sbjct: 481 YEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLGQKNIIFLI 540

Query: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDR 600
           NNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAI+IAMKDR
Sbjct: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIEIAMKDR 600

Query: 601 NDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPPKLLKN 640
           NDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPPKLLKN
Sbjct: 601 NDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPPKLLKN 640

BLAST of Chy8G152270 vs. NCBI nr
Match: TYK29658.1 (pyruvate decarboxylase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1210 bits (3130), Expect = 0.0
Identity = 596/640 (93.12%), Postives = 617/640 (96.41%), Query Frame = 0

Query: 1   MGITMWINSLGIRQLLPINKLQNGYHFGTQTRPMVAYSPRIECVTNGSLASDKIISSKNN 60
           MGITMWINSLGI Q+LP+NKL    HF TQTRPM AY+PRIE VTNGSLASDK+ISS NN
Sbjct: 1   MGITMWINSLGISQILPMNKLN---HFQTQTRPMAAYNPRIEHVTNGSLASDKLISSNNN 60

Query: 61  GSPLVIVPPPTTAPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120
           GSPLVIVPPP T PSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC
Sbjct: 61  GSPLVIVPPPVTVPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120

Query: 121 NELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS 180
           NELNAGYAADGYAR+RGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS
Sbjct: 121 NELNAGYAADGYARNRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS 180

Query: 181 KKILHHTIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICC 240
           KKILHHTIGLPDFSQELRCFQNVTC+QA+ID+LEDAQWQIDKAICKCLEESKPVYISICC
Sbjct: 181 KKILHHTIGLPDFSQELRCFQNVTCYQAVIDNLEDAQWQIDKAICKCLEESKPVYISICC 240

Query: 241 NLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300
           NLVAIPHPSFSA+PLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA
Sbjct: 241 NLVAIPHPSFSAKPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300

Query: 301 EAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360
           EAAFL+LA++CGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA
Sbjct: 301 EAAFLELANTCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360

Query: 361 TLDELETVGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYEN 420
           TLDELETVGYSLTYKKN+AI+VKPD VVFPNG SYG VQ+KDFL ALGKRLKPNLRAYEN
Sbjct: 361 TLDELETVGYSLTYKKNKAIMVKPDGVVFPNGASYGGVQIKDFLRALGKRLKPNLRAYEN 420

Query: 421 YRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 480
           YRRIYIAES PPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG
Sbjct: 421 YRRIYIAESGPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 480

Query: 481 YEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLI 540
           YE+QLLYASIGWSLGATLGYAQ+AP KRL+LCIGDGSFQMAPQDVSTMLKL Q NIIFLI
Sbjct: 481 YEIQLLYASIGWSLGATLGYAQSAPQKRLVLCIGDGSFQMAPQDVSTMLKLGQNNIIFLI 540

Query: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDR 600
           NNGGYTIEVEIHDGPYNIINNWDYTAFV+AVNNHQ NCWTTKVHTEEELV AI+IAM+DR
Sbjct: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVEAVNNHQGNCWTTKVHTEEELVRAIEIAMEDR 600

Query: 601 NDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPPKLLKN 640
           ND LCFIEVI HRDDTSKELLEFGSRIAAMGSHPPKLLKN
Sbjct: 601 NDYLCFIEVIVHRDDTSKELLEFGSRIAAMGSHPPKLLKN 637

BLAST of Chy8G152270 vs. NCBI nr
Match: XP_008464214.1 (PREDICTED: LOW QUALITY PROTEIN: pyruvate decarboxylase 1-like [Cucumis melo])

HSP 1 Score: 1209 bits (3127), Expect = 0.0
Identity = 595/640 (92.97%), Postives = 616/640 (96.25%), Query Frame = 0

Query: 1   MGITMWINSLGIRQLLPINKLQNGYHFGTQTRPMVAYSPRIECVTNGSLASDKIISSKNN 60
           MGITMWINSLGI Q+LP+NKL    HF TQTRPM AY+PRIE VTNGSLASDK+ISS NN
Sbjct: 1   MGITMWINSLGISQILPMNKLN---HFQTQTRPMAAYNPRIEHVTNGSLASDKLISSNNN 60

Query: 61  GSPLVIVPPPTTAPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120
           GSPLVIVPPP T PSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC
Sbjct: 61  GSPLVIVPPPVTVPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120

Query: 121 NELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS 180
           NELNAGYAADGYAR+RGVGACAVTFTVGSLSLI+AIAGAYSEDLPVICIVGGPNSNDYGS
Sbjct: 121 NELNAGYAADGYARNRGVGACAVTFTVGSLSLISAIAGAYSEDLPVICIVGGPNSNDYGS 180

Query: 181 KKILHHTIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICC 240
           KKILHHTIGLPDFSQELRCFQNVTC+QA+ID+LEDAQWQIDKAICKCLEESKPVYISICC
Sbjct: 181 KKILHHTIGLPDFSQELRCFQNVTCYQAVIDNLEDAQWQIDKAICKCLEESKPVYISICC 240

Query: 241 NLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300
           NLVAIPHPSFSA+PLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA
Sbjct: 241 NLVAIPHPSFSAKPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300

Query: 301 EAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360
           EAAFL+LA++CGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA
Sbjct: 301 EAAFLELANTCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360

Query: 361 TLDELETVGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYEN 420
           TLDELETVG SLTYKKN+AI+VKPD VVFPNG SYG VQMKDFL ALGKRLKPNLRAYEN
Sbjct: 361 TLDELETVGXSLTYKKNKAIMVKPDGVVFPNGASYGGVQMKDFLRALGKRLKPNLRAYEN 420

Query: 421 YRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 480
           YRRIYIAES PPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG
Sbjct: 421 YRRIYIAESGPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 480

Query: 481 YEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLI 540
           YE+QLLYASIGWSLGATLGYAQ+AP KRL+LCIGDGSFQMAPQDVSTMLKL Q NIIFLI
Sbjct: 481 YEIQLLYASIGWSLGATLGYAQSAPQKRLVLCIGDGSFQMAPQDVSTMLKLGQNNIIFLI 540

Query: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDR 600
           NNGGYTIEVEIHDGPYNIINNWDYTAFV+AVNNHQ NCWTTKVHTEEELV AI+IAM+DR
Sbjct: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVEAVNNHQGNCWTTKVHTEEELVRAIEIAMEDR 600

Query: 601 NDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPPKLLKN 640
           NDCLCF EVI HRDDTSKELLEFGSRIAAMGSHPPKLLKN
Sbjct: 601 NDCLCFTEVIVHRDDTSKELLEFGSRIAAMGSHPPKLLKN 637

BLAST of Chy8G152270 vs. NCBI nr
Match: KAA0037740.1 (pyruvate decarboxylase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1098 bits (2841), Expect = 0.0
Identity = 554/640 (86.56%), Postives = 573/640 (89.53%), Query Frame = 0

Query: 1   MGITMWINSLGIRQLLPINKLQNGYHFGTQTRPMVAYSPRIECVTNGSLASDKIISSKNN 60
           MGITMWINSLGI Q+LP+NKL    HF TQTRPM AY+PRIE VTNGSLASDK+ISS NN
Sbjct: 1   MGITMWINSLGISQILPMNKLN---HFQTQTRPMAAYNPRIEHVTNGSLASDKLISSNNN 60

Query: 61  GSPLVIVPPPTTAPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120
           GSPLVIVPPP T PSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC
Sbjct: 61  GSPLVIVPPPVTVPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCC 120

Query: 121 NELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGS 180
           NELNAGYAADGYAR+RGVGACAVTFTVGSLSLI+AIAGAYSEDLPVICIVGGPNSNDYGS
Sbjct: 121 NELNAGYAADGYARNRGVGACAVTFTVGSLSLISAIAGAYSEDLPVICIVGGPNSNDYGS 180

Query: 181 KKILHHTIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICC 240
           KKILHHTIGLPDFSQELRCFQNVTC+QA+ID+LEDAQWQIDKAICKCLEESKPVYISICC
Sbjct: 181 KKILHHTIGLPDFSQELRCFQNVTCYQAVIDNLEDAQWQIDKAICKCLEESKPVYISICC 240

Query: 241 NLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300
           NLVAIPHPSFSA+PLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA
Sbjct: 241 NLVAIPHPSFSAKPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKA 300

Query: 301 EAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360
           EAAFL+LA++CGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA
Sbjct: 301 EAAFLELANTCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGA 360

Query: 361 TLDELETVGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYEN 420
           TLDELET                                  DFL ALGKRLKPNLRAYEN
Sbjct: 361 TLDELET----------------------------------DFLRALGKRLKPNLRAYEN 420

Query: 421 YRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCG 480
           YRRIYIAES PPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSW             
Sbjct: 421 YRRIYIAESGPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSW------------- 480

Query: 481 YEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLI 540
           YE+QLLYASIGWSLGATLGYAQ+AP KRL+LCIGDGSFQMAPQDVSTMLKL Q NIIFLI
Sbjct: 481 YEIQLLYASIGWSLGATLGYAQSAPQKRLVLCIGDGSFQMAPQDVSTMLKLGQNNIIFLI 540

Query: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDR 600
           NNGGYTIEVEIHDGPYNIINNWDYTAFV+AVNNHQ NCWTTKVHTEEELV AI+IAM+DR
Sbjct: 541 NNGGYTIEVEIHDGPYNIINNWDYTAFVEAVNNHQGNCWTTKVHTEEELVRAIEIAMEDR 590

Query: 601 NDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPPKLLKN 640
           NDCLCF EVI HRDDTSKELLEFGSRIAAMGSHPPKLLKN
Sbjct: 601 NDCLCFTEVIVHRDDTSKELLEFGSRIAAMGSHPPKLLKN 590

BLAST of Chy8G152270 vs. NCBI nr
Match: XP_038881465.1 (pyruvate decarboxylase 1-like [Benincasa hispida])

HSP 1 Score: 1074 bits (2778), Expect = 0.0
Identity = 523/623 (83.95%), Postives = 570/623 (91.49%), Query Frame = 0

Query: 18  INKLQNGYHFGTQTRPMVAYSPRIECVTNGSLASDKIISSK--NNGSPLVIVPPPTTAPS 77
           +N L + +HF T+ R M AY P I+ VT+ S+ASDK+I SK  +N  P VI+PPPTT PS
Sbjct: 1   MNNLPDAFHFKTEGRIMAAYCPGIQPVTSASIASDKLICSKPRSNNGPRVIIPPPTTVPS 60

Query: 78  TLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCCNELNAGYAADGYARS 137
           TLGHYLARRLV+IGVSDIFSVPGDSNL+LFDYFVAEKGLNLVGCCNELNAGYAADGYAR 
Sbjct: 61  TLGHYLARRLVQIGVSDIFSVPGDSNLMLFDYFVAEKGLNLVGCCNELNAGYAADGYARR 120

Query: 138 RGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLPDFSQ 197
           RGVGACAVTFTVGSLSLIN+IAGAYSEDLPVICIVGGPNSNDY SKKILHHTIGLPDF+Q
Sbjct: 121 RGVGACAVTFTVGSLSLINSIAGAYSEDLPVICIVGGPNSNDYRSKKILHHTIGLPDFTQ 180

Query: 198 ELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICCNLVAIPHPSFSAQPL 257
           E RCFQNVTCHQ IID+LEDAQWQID+AI KCLEESKPVYISICCNLVAIPHPSFSAQ L
Sbjct: 181 EFRCFQNVTCHQVIIDNLEDAQWQIDRAISKCLEESKPVYISICCNLVAIPHPSFSAQQL 240

Query: 258 IPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKAEAAFLKLADSCGYAV 317
           IPLSLSPKQSNQ+ L+MAVEKA+DLLNTA+KPVMIGGKKLR AKA+A FL+LAD+CGYAV
Sbjct: 241 IPLSLSPKQSNQLCLKMAVEKASDLLNTAVKPVMIGGKKLRLAKAQAVFLELADACGYAV 300

Query: 318 AVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGATLDELETVGYSLTYK 377
           AVTPSAKGMFPENHPHFIGTYWGT+STAFCGETVEIADASIF+GATLDELET+GYSLTYK
Sbjct: 301 AVTPSAKGMFPENHPHFIGTYWGTISTAFCGETVEIADASIFIGATLDELETIGYSLTYK 360

Query: 378 KNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYENYRRIYIAESSPPESE 437
           KN+AIIV+P+ V+FPNG  +G + MKDFL AL KRLKPNL AYENYRRIYI ES PP+SE
Sbjct: 361 KNKAIIVEPNRVMFPNGSCFGLILMKDFLQALTKRLKPNLTAYENYRRIYIPESDPPQSE 420

Query: 438 AGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCGYEVQLLYASIGWSLG 497
            GE LRVNVLFKHIQKML++NMTVI+ETGDSWFHSQKLKLPKSCGYE+QLLYASIGWSLG
Sbjct: 421 LGEALRVNVLFKHIQKMLTNNMTVISETGDSWFHSQKLKLPKSCGYEIQLLYASIGWSLG 480

Query: 498 ATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLINNGGYTIEVEIHDGP 557
           ATLGYAQAAP +R++LCIGDGSFQM PQDVSTML+L Q NIIFLINNGGYTIEVEIHDGP
Sbjct: 481 ATLGYAQAAPKERVVLCIGDGSFQMTPQDVSTMLRLGQNNIIFLINNGGYTIEVEIHDGP 540

Query: 558 YNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDRNDCLCFIEVIAHRDD 617
           YN+I NWDYTA VDA++N + NCWTTKVHTEEELVNAI+IA  DRNDCLCFIEVI HRDD
Sbjct: 541 YNVIKNWDYTAIVDAMDNREGNCWTTKVHTEEELVNAIEIATGDRNDCLCFIEVIVHRDD 600

Query: 618 TSKELLEFGSRIAAMGSHPPKLL 638
           TSKELLEFGS+IAAMGS P  LL
Sbjct: 601 TSKELLEFGSKIAAMGSRPSNLL 623

BLAST of Chy8G152270 vs. TAIR 10
Match: AT5G01320.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 793.1 bits (2047), Expect = 1.6e-229
Identity = 382/568 (67.25%), Postives = 447/568 (78.70%), Query Frame = 0

Query: 68  PPPTTAPSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCCNELNAGY 127
           P  TT+ STLG +L+RRLV+ GV+D+FSVPGD NL L D+ +AE  LN +GCCNELNAGY
Sbjct: 34  PITTTSESTLGRHLSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEPELNNIGCCNELNAGY 93

Query: 128 AADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHT 187
           AADGYARSRGVGAC VTFTVG LS++NAIAGAYSE+LPVICIVGGPNSND+G+ +ILHHT
Sbjct: 94  AADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHT 153

Query: 188 IGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICCNLVAIPH 247
           IGLPDFSQELRCFQ VTC+QA++++LEDA  QIDKAI   L+ESKPVYISI CNL A PH
Sbjct: 154 IGLPDFSQELRCFQTVTCYQAVVNNLEDAHEQIDKAIATALKESKPVYISISCNLAATPH 213

Query: 248 PSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKAEAAFLKL 307
           P+F+  P +P  L+P+ SN MGLE AVE   + LN A+KPVM+GG KLR AKA  AFL+L
Sbjct: 214 PTFARDP-VPFDLTPRMSNTMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKASEAFLEL 273

Query: 308 ADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGATLDELET 367
           AD+ GY +AV PS KG+ PENHPHFIGTYWG VST FC E VE ADA IF G   ++  +
Sbjct: 274 ADASGYPLAVMPSTKGLVPENHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSS 333

Query: 368 VGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYENYRRIYIA 427
           VGYSL  KK +AIIV PD VV  NG ++G V M DF   L KR+K N  AYENY RI++ 
Sbjct: 334 VGYSLLLKKEKAIIVHPDRVVVANGPTFGCVLMSDFFRELAKRVKRNETAYENYERIFVP 393

Query: 428 ESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCGYEVQLLY 487
           E  P + + GE LRVN +F+HIQKMLSS   VIAETGDSWF+ QKLKLPK CGYE Q+ Y
Sbjct: 394 EGKPLKCKPGEPLRVNAMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQY 453

Query: 488 ASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLINNGGYTI 547
            SIGWS+GATLGYAQA P KR+L  IGDGSFQ+  QD+STM++  QK IIFLINNGGYTI
Sbjct: 454 GSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDISTMIRNGQKAIIFLINNGGYTI 513

Query: 548 EVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDRNDCLCFI 607
           EVEIHDGPYN+I NW+YT  VDA++N +  CWTTKV  EEELV AIK A  ++ D LCFI
Sbjct: 514 EVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAIKTATTEKKDSLCFI 573

Query: 608 EVIAHRDDTSKELLEFGSRIAAMGSHPP 636
           EVI H+DDTSKELLE+GSR++A    PP
Sbjct: 574 EVIVHKDDTSKELLEWGSRVSAANGRPP 600

BLAST of Chy8G152270 vs. TAIR 10
Match: AT4G33070.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 789.6 bits (2038), Expect = 1.8e-228
Identity = 377/561 (67.20%), Postives = 449/561 (80.04%), Query Frame = 0

Query: 75  STLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCCNELNAGYAADGYAR 134
           +TLG +LARRLV+ GV+D+FSVPGD NL L D+ +AE  LNL+GCCNELNAGYAADGYAR
Sbjct: 45  ATLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYAR 104

Query: 135 SRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLPDFS 194
           SRGVGAC VTFTVG LS++NAIAGAYSE+LP+ICIVGGPNSNDYG+ +ILHHTIGLPDFS
Sbjct: 105 SRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFS 164

Query: 195 QELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESKPVYISICCNLVAIPHPSFSAQP 254
           QELRCFQ VTC+QA++++L+DA  QIDKAI   L+ESKPVYIS+ CNL AIPH +FS  P
Sbjct: 165 QELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALKESKPVYISVSCNLAAIPHHTFSRDP 224

Query: 255 LIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKAEAAFLKLADSCGYA 314
            +P SL+P+ SN+MGLE AVE   + LN A+KPVM+GG KLR AKA  AF++LAD+ GYA
Sbjct: 225 -VPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKACDAFVELADASGYA 284

Query: 315 VAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGATLDELETVGYSLTY 374
           +A+ PSAKG  PE+HPHFIGTYWG VST FC E VE ADA IF G   ++  +VGYSL  
Sbjct: 285 LAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLL 344

Query: 375 KKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNLRAYENYRRIYIAESSPPES 434
           KK +AI+V+PD +   NG ++G + M DF   L KR+K N  AYENY RI++ E  P + 
Sbjct: 345 KKEKAIVVQPDRITVANGPTFGCILMSDFFRELSKRVKRNETAYENYHRIFVPEGKPLKC 404

Query: 435 EAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCGYEVQLLYASIGWSL 494
           E+ E LRVN +F+HIQKMLSS   VIAETGDSWF+ QKLKLPK CGYE Q+ Y SIGWS+
Sbjct: 405 ESREPLRVNTMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSV 464

Query: 495 GATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLEQKNIIFLINNGGYTIEVEIHDG 554
           GATLGYAQA+P KR+L  IGDGSFQ+  QD+STML+  QK IIFLINNGGYTIEVEIHDG
Sbjct: 465 GATLGYAQASPEKRVLAFIGDGSFQVTVQDISTMLRNGQKTIIFLINNGGYTIEVEIHDG 524

Query: 555 PYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIKIAMKDRNDCLCFIEVIAHRD 614
           PYN+I NW+YT  VDA++N + NCWT KV  EEELV AI  A  ++ DCLCFIEVI H+D
Sbjct: 525 PYNVIKNWNYTGLVDAIHNGEGNCWTAKVRYEEELVEAITTATTEKKDCLCFIEVILHKD 584

Query: 615 DTSKELLEFGSRIAAMGSHPP 636
           DTSKELLE+GSR++A  S PP
Sbjct: 585 DTSKELLEWGSRVSAANSRPP 604

BLAST of Chy8G152270 vs. TAIR 10
Match: AT5G54960.1 (pyruvate decarboxylase-2 )

HSP 1 Score: 778.1 bits (2008), Expect = 5.5e-225
Identity = 381/583 (65.35%), Postives = 452/583 (77.53%), Query Frame = 0

Query: 55  ISSKNNGSPLVIVPPPTTAP--STLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFVAEK 114
           +S+  N SPL      T +P  +TLG YLARRLVEIGV+D+FSVPGD NL L D+ +AE 
Sbjct: 26  VSTVQNTSPL---HSTTVSPCDATLGRYLARRLVEIGVTDVFSVPGDFNLTLLDHLIAEP 85

Query: 115 GLNLVGCCNELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGG 174
            L L+GCCNELNAGYAADGYARSRGVGAC VTFTVG LS++NAIAGAYSE+LP+ICIVGG
Sbjct: 86  NLKLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGG 145

Query: 175 PNSNDYGSKKILHHTIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEESK 234
           PNSNDYG+ +ILHHTIGLPDF+QELRCFQ VTC QA+I++LE+A   ID AI   L+ESK
Sbjct: 146 PNSNDYGTNRILHHTIGLPDFTQELRCFQAVTCFQAVINNLEEAHELIDTAISTALKESK 205

Query: 235 PVYISICCNLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGG 294
           PVYISI CNL AIP P+FS  P +P  L  K SNQ+GL+ AVE AA+ LN A+KPV++GG
Sbjct: 206 PVYISISCNLPAIPLPTFSRHP-VPFMLPMKVSNQIGLDAAVEAAAEFLNKAVKPVLVGG 265

Query: 295 KKLRPAKAEAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIA 354
            K+R AKA  AF++LAD+ GY +AV PSAKG  PE+H HFIGTYWG VSTAFC E VE A
Sbjct: 266 PKMRVAKAADAFVELADASGYGLAVMPSAKGQVPEHHKHFIGTYWGAVSTAFCAEIVESA 325

Query: 355 DASIFVGATLDELETVGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLK 414
           DA +F G   ++  +VGYSL  KK +AIIV+PD V   NG ++G V MKDFL  L KR+K
Sbjct: 326 DAYLFAGPIFNDYSSVGYSLLLKKEKAIIVQPDRVTIGNGPAFGCVLMKDFLSELAKRIK 385

Query: 415 PNLRAYENYRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQK 474
            N  +YENY RIY+ E  P      E LRVNVLF+HIQ MLSS   V+AETGDSWF+ QK
Sbjct: 386 HNNTSYENYHRIYVPEGKPLRDNPNESLRVNVLFQHIQNMLSSESAVLAETGDSWFNCQK 445

Query: 475 LKLPKSCGYEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLE 534
           LKLP+ CGYE Q+ Y SIGWS+GATLGYAQA P++R++ CIGDGSFQ+  QDVSTM++  
Sbjct: 446 LKLPEGCGYEFQMQYGSIGWSVGATLGYAQAMPNRRVIACIGDGSFQVTAQDVSTMIRCG 505

Query: 535 QKNIIFLINNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNA 594
           QK IIFLINNGGYTIEVEIHDGPYN+I NW+YTAFV+A++N +  CWT KV  EEELV A
Sbjct: 506 QKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTAFVEAIHNGEGKCWTAKVRCEEELVKA 565

Query: 595 IKIAMKDRNDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPP 636
           I  A  +  +  CFIEVI H+DDTSKELLE+GSR++A  S PP
Sbjct: 566 INTATNEEKESFCFIEVIVHKDDTSKELLEWGSRVSAANSRPP 604

BLAST of Chy8G152270 vs. TAIR 10
Match: AT5G01330.1 (pyruvate decarboxylase-3 )

HSP 1 Score: 777.3 bits (2006), Expect = 9.4e-225
Identity = 381/586 (65.02%), Postives = 449/586 (76.62%), Query Frame = 0

Query: 56  SSKNNGSPLVIVPPPTTA------PSTLGHYLARRLVEIGVSDIFSVPGDSNLVLFDYFV 115
           S  +NG   +    PT A       +TLG +L+RRLV+ GV+DIF+VPGD NL L D  +
Sbjct: 5   SLPSNGVATIQDSAPTAATILGSSAATLGRHLSRRLVQAGVTDIFTVPGDFNLSLLDQLI 64

Query: 116 AEKGLNLVGCCNELNAGYAADGYARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICI 175
           A   LN +GCCNELNAGYAADGYARSRGVGAC VTFTVG LS++NAIAGAYSE+LPVICI
Sbjct: 65  ANPELNNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICI 124

Query: 176 VGGPNSNDYGSKKILHHTIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLE 235
           VGGPNSND+G+ +ILHHTIGLPDFSQELRCFQ VTC+QA+++ LEDA  QIDKAI   L 
Sbjct: 125 VGGPNSNDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNHLEDAHEQIDKAIATALR 184

Query: 236 ESKPVYISICCNLVAIPHPSFSAQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVM 295
           ESKPVYISI CNL AIPHP+F++ P +P  L+P+ SN+  LE AVE   + LN A+KPVM
Sbjct: 185 ESKPVYISISCNLAAIPHPTFASYP-VPFDLTPRLSNKDCLEAAVEATLEFLNKAVKPVM 244

Query: 296 IGGKKLRPAKAEAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETV 355
           +GG KLR AKA  AF++LAD+ GY VAV PSAKG  PENHPHFIGTYWG VST FC E V
Sbjct: 245 VGGPKLRVAKARDAFVELADASGYPVAVMPSAKGFVPENHPHFIGTYWGAVSTLFCSEIV 304

Query: 356 EIADASIFVGATLDELETVGYSLTYKKNQAIIVKPDSVVFPNGESYGAVQMKDFLWALGK 415
           E ADA IF G   ++  +VGYSL  KK +AIIV PDSVV  NG ++G V+M +F   L K
Sbjct: 305 ESADAYIFAGPIFNDYSSVGYSLLLKKEKAIIVHPDSVVVANGPTFGCVRMSEFFRELAK 364

Query: 416 RLKPNLRAYENYRRIYIAESSPPESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFH 475
           R+KPN  AYENY RI++ E  P + +  E LR+N +F+HIQKMLS+   VIAETGDSWF+
Sbjct: 365 RVKPNKTAYENYHRIFVPEGKPLKCKPREPLRINAMFQHIQKMLSNETAVIAETGDSWFN 424

Query: 476 SQKLKLPKSCGYEVQLLYASIGWSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTML 535
            QKLKLPK CGYE Q+ Y SIGWS+GATLGYAQA P KR+L  IGDGSFQ+  QDVSTM+
Sbjct: 425 CQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDVSTMI 484

Query: 536 KLEQKNIIFLINNGGYTIEVEIHDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEEL 595
           +  QK IIFLINNGGYTIEVEIHDGPYN+I NW+YT  VDA++N +  CWTTKV  EEEL
Sbjct: 485 RNGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEEL 544

Query: 596 VNAIKIAMKDRNDCLCFIEVIAHRDDTSKELLEFGSRIAAMGSHPP 636
           V AI  A  ++ D LCFIEVI H+DDTSKELLE+GSR++A    PP
Sbjct: 545 VEAINTATLEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANGRPP 589

BLAST of Chy8G152270 vs. TAIR 10
Match: AT5G17380.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 88.6 bits (218), Expect = 2.0e-17
Identity = 120/515 (23.30%), Postives = 208/515 (40.39%), Query Frame = 0

Query: 71  TTAPSTLGHYL-ARRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCCNELNAGYAA 130
           TT PS  G+ L A+ L  +GV+ +F V G     L    +A  G+  +   NE +AGYAA
Sbjct: 7   TTPPSIDGNVLVAKSLSHLGVTHMFGVVGIPVTSLASRAMA-LGIRFIAFHNEQSAGYAA 66

Query: 131 DGYARSRGVGACAVTFTVGSLSLINAIAG---AYSEDLPVICIVGGPNSNDYGSKKILHH 190
             Y      G   +  TV     ++ +AG   A+    P++ I G  +  D         
Sbjct: 67  SAYGYL--TGKPGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCDQRD--------- 126

Query: 191 TIGLPDFSQELRCFQNVTCHQAIIDSLEDAQWQIDKAICKCLEES---KP--VYISICCN 250
            +G  DF QEL   + V     + +  +D + +I   + + L+ +   +P   Y+ I  +
Sbjct: 127 -VGRGDF-QELDQIEAVKAFSKLSEKAKDVR-EIPDCVSRVLDRAVSGRPGGCYLDIPTD 186

Query: 251 LVAIPHPSFSAQPLI-PLSLSPKQSNQMG-LEMAVEKAADLLNTAIKPVMIGGKKLRPAK 310
           ++        A  L+  +  S K+    G L   +E A  LL  A +P+++ GK    ++
Sbjct: 187 VLRQKISESEADKLVDEVERSRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAAYSR 246

Query: 311 AEAAFLKLADSCGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVG 370
           AE    KL +  G     TP  KG+ P+ H  F  T   +++   C       D ++ VG
Sbjct: 247 AEDELKKLVEITGIPFLPTPMGKGLLPDTH-EFSATAARSLAIGKC-------DVALVVG 306

Query: 371 ATLDELETVGYSLTYKKNQAIIV-----------KPDSVVFPNGESYGAV---QMKDFLW 430
           A L+ L   G S  + K+   I+           KP   +  + ++   +   ++KD  +
Sbjct: 307 ARLNWLLHFGESPKWDKDVKFILVDVSEEEIELRKPHLGIVGDAKTVIGLLNREIKDDPF 366

Query: 431 ALGKRLKPNLRAYENYRRIYIAESSPPESEAGEELRVN-----VLFKHIQKMLSSNMTVI 490
            LGK               ++   S    E GE++ +      V F  +  M      ++
Sbjct: 367 CLGK------------SNSWVESISKKAKENGEKMEIQLAKDVVPFNFLTPMRIIRDAIL 426

Query: 491 AETGDSWFHSQKLKLPKSCGYEVQL-----------LYASIGWSLGATLGYAQAAPHKRL 545
           A  G S     +       G  V +            + ++G  LG  +  A A+P + +
Sbjct: 427 AVEGPSPVVVSEGANTMDVGRSVLVQKEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLV 486

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A2Y5L98.0e-22965.91Pyruvate decarboxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=PDC1 PE=2 SV=... [more]
A2XFI31.8e-22866.14Pyruvate decarboxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=PDC2 PE=2 SV=... [more]
Q0DHF62.3e-22865.91Pyruvate decarboxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC1 PE=2 S... [more]
Q9M0402.3e-22867.25Pyruvate decarboxylase 4 OS=Arabidopsis thaliana OX=3702 GN=PDC4 PE=2 SV=1[more]
O826472.6e-22767.20Pyruvate decarboxylase 1 OS=Arabidopsis thaliana OX=3702 GN=PDC1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L1C60.0e+0097.34Pyruvate decarboxylase OS=Cucumis sativus OX=3659 GN=Csa_4G312250 PE=3 SV=1[more]
A0A5D3E1K90.0e+0093.13Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold6... [more]
A0A1S3CKY90.0e+0092.97Pyruvate decarboxylase OS=Cucumis melo OX=3656 GN=LOC103502149 PE=3 SV=1[more]
A0A5A7T2Y20.0e+0086.56Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1... [more]
A0A5D3E0N51.8e-29578.28Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold6... [more]
Match NameE-valueIdentityDescription
XP_004148631.30.097.34pyruvate decarboxylase 1 [Cucumis sativus] >KGN54392.1 hypothetical protein Csa_... [more]
TYK29658.10.093.13pyruvate decarboxylase 1-like [Cucumis melo var. makuwa][more]
XP_008464214.10.092.97PREDICTED: LOW QUALITY PROTEIN: pyruvate decarboxylase 1-like [Cucumis melo][more]
KAA0037740.10.086.56pyruvate decarboxylase 1-like [Cucumis melo var. makuwa][more]
XP_038881465.10.083.95pyruvate decarboxylase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G01320.11.6e-22967.25Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT4G33070.11.8e-22867.20Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT5G54960.15.5e-22565.35pyruvate decarboxylase-2 [more]
AT5G01330.19.4e-22565.02pyruvate decarboxylase-3 [more]
AT5G17380.12.0e-1723.30Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePIRSFPIRSF036565Pyruvt_ip_decrbcoord: 71..632
e-value: 3.6E-192
score: 637.4
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePANTHERPTHR43452PYRUVATE DECARBOXYLASEcoord: 70..636
IPR012001Thiamine pyrophosphate enzyme, N-terminal TPP-binding domainPFAMPF02776TPP_enzyme_Ncoord: 76..243
e-value: 3.3E-33
score: 114.7
NoneNo IPR availableGENE3D3.40.50.970coord: 73..249
e-value: 1.2E-58
score: 199.7
NoneNo IPR availableGENE3D3.40.50.1220coord: 263..426
e-value: 3.4E-38
score: 132.8
NoneNo IPR availableGENE3D3.40.50.970coord: 434..620
e-value: 5.0E-50
score: 171.6
NoneNo IPR availablePIRSRPIRSR036565-2PIRSR036565-2coord: 76..620
e-value: 1.0E-165
score: 549.8
NoneNo IPR availablePANTHERPTHR43452:SF25PYRUVATE DECARBOXYLASE 1coord: 70..636
NoneNo IPR availableCDDcd02005TPP_PDC_IPDCcoord: 440..621
e-value: 2.57829E-78
score: 244.751
NoneNo IPR availableCDDcd07038TPP_PYR_PDC_IPDC_likecoord: 80..240
e-value: 6.57477E-88
score: 268.979
IPR012000Thiamine pyrophosphate enzyme, central domainPFAMPF00205TPP_enzyme_Mcoord: 274..385
e-value: 2.4E-21
score: 75.9
IPR011766Thiamine pyrophosphate enzyme, C-terminal TPP-bindingPFAMPF02775TPP_enzyme_Ccoord: 484..608
e-value: 2.8E-12
score: 46.7
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 76..251
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 440..632
IPR029035DHS-like NAD/FAD-binding domain superfamilySUPERFAMILY52467DHS-like NAD/FAD-binding domaincoord: 269..414

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy8G152270.1Chy8G152270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004737 pyruvate decarboxylase activity
molecular_function GO:0030976 thiamine pyrophosphate binding
molecular_function GO:0016831 carboxy-lyase activity
molecular_function GO:0003824 catalytic activity