Chy8G149490 (gene) Cucumber (hystrix) v1

Overview


Name	Chy8G149490
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	cysteine proteinase inhibitor A-like
Location	chrH08: 8145117 .. 8145353 (+)
RNA-Seq Expression	Chy8G149490
Synteny	Chy8G149490

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGATCATGTCCCCTCTTGAACCGATTAAGGATATCAATGATCCGGAAGTGCAACAAATAGGAAACTTAACAGTGGATGACCATAACAAGAAAACAGGTGAACATCTGAAGTTCGTGCGTGTGGTGAATGGTTTATCTGCACAATTTGTTGACCCATTTTCTCCTCTTGGAGGGACACTATATGAGCTTGTGTTGGAGGCTATAAACGCCGGAAAAATAAATTGGACATATAAG

mRNA sequence

ATGTCGATCATGTCCCCTCTTGAACCGATTAAGGATATCAATGATCCGGAAGTGCAACAAATAGGAAACTTAACAGTGGATGACCATAACAAGAAAACAGGTGAACATCTGAAGTTCGTGCGTGTGGTGAATGGTTTATCTGCACAATTTGTTGACCCATTTTCTCCTCTTGGAGGGACACTATATGAGCTTGTGTTGGAGGCTATAAACGCCGGAAAAATAAATTGGACATATAAG

Coding sequence (CDS)

ATGTCGATCATGTCCCCTCTTGAACCGATTAAGGATATCAATGATCCGGAAGTGCAACAAATAGGAAACTTAACAGTGGATGACCATAACAAGAAAACAGGTGAACATCTGAAGTTCGTGCGTGTGGTGAATGGTTTATCTGCACAATTTGTTGACCCATTTTCTCCTCTTGGAGGGACACTATATGAGCTTGTGTTGGAGGCTATAAACGCCGGAAAAATAAATTGGACATATAAG

Protein sequence

MSIMSPLEPIKDINDPEVQQIGNLTVDDHNKKTGEHLKFVRVVNGLSAQFVDPFSPLGGTLYELVLEAINAGKINWTYK

Homology

[Close]

BLAST of Chy8G149490 vs. ExPASy Swiss-Prot
Match: Q84WT8 (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 1.2e-04
Identity = 22/65 (33.85%), Postives = 39/65 (60.00%), Query Frame = 0


Query: 8  EPIKDINDPEVQQIGNLTVDDHNKKTGEHLKFVRVVNGLSAQFVDPFSPLGGTLYELVLE 67
          +PIK+++DP+V  +    +++HNK++ E L FV+VV G +         + GT Y+L + 
Sbjct: 33 KPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTT-------QVVSGTKYDLKIA 90

Query: 68 AINAG 73
          A + G
Sbjct: 93 AKDGG 90

[Close]

BLAST of Chy8G149490 vs. ExPASy Swiss-Prot
Match: P37842 (Multicystatin OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 44.3 bits (103), Expect = 7.5e-04
Identity = 25/60 (41.67%), Postives = 33/60 (55.00%), Query Frame = 0


Query: 14  NDPEVQQIGNLTVDDHNKKTGEHLKFVRVVNGLSAQFVDPFSPLGGTLYELVLEAINAGK 73
           N+PE Q +    V D+NKK   HL+FV  +N +  Q V       G LY + L AI+AGK
Sbjct: 486 NNPEFQDLARFAVQDYNKKENAHLEFVENLN-VKEQLV------AGMLYYITLVAIDAGK 538

[Close]

BLAST of Chy8G149490 vs. ExPASy TrEMBL
Match: A0A5A7TJK0 (Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001140 PE=4 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.1e-26
Identity = 63/80 (78.75%), Postives = 68/80 (85.00%), Query Frame = 0


Query: 1  MSIMSPLEPIKDINDP-EVQQIGNLTVDDHNKKTGEHLKFVRVVNGLSAQFVDPFSPLGG 60
          MSIMS  +PI+DIND  EVQ +GNLTVDDHNKKTGE+LKFVRVVNGL+A F DP SPLGG
Sbjct: 1  MSIMSAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG 60

Query: 61 TLYELVLEAINAGKINWTYK 80
           LYELVLEAI   KINWTYK
Sbjct: 61 VLYELVLEAITTSKINWTYK 80

[Close]

BLAST of Chy8G149490 vs. ExPASy TrEMBL
Match: A0A1S3B5B9 (cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=4 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.1e-26
Identity = 63/80 (78.75%), Postives = 68/80 (85.00%), Query Frame = 0


Query: 1  MSIMSPLEPIKDINDP-EVQQIGNLTVDDHNKKTGEHLKFVRVVNGLSAQFVDPFSPLGG 60
          MSIMS  +PI+DIND  EVQ +GNLTVDDHNKKTGE+LKFVRVVNGL+A F DP SPLGG
Sbjct: 1  MSIMSAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG 60

Query: 61 TLYELVLEAINAGKINWTYK 80
           LYELVLEAI   KINWTYK
Sbjct: 61 VLYELVLEAITTSKINWTYK 80

[Close]

BLAST of Chy8G149490 vs. ExPASy TrEMBL
Match: A0A5D3C2L1 (Proteinase inhibitor I25, cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001230 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.6e-10
Identity = 41/74 (55.41%), Postives = 52/74 (70.27%), Query Frame = 0


Query: 8  EPIKDINDP-EVQQ-IGNLTVDDHNKKTGEHLKFVRVVNGLSAQ-FVDPFSPLGGTLYEL 67
          +PI++IND  +VQ  IG L V+DHNKKTGE+L+FV VVNGL ++ FV P     G LY L
Sbjct: 11 KPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGLRSEVFVQPGCTHEGILYHL 70

Query: 68 VLEAINAGKINWTY 79
          ++EA     INWTY
Sbjct: 71 LVEAKTIEGINWTY 84

[Close]

BLAST of Chy8G149490 vs. ExPASy TrEMBL
Match: A0A0A0L3W1 (Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.6e-10
Identity = 42/74 (56.76%), Postives = 51/74 (68.92%), Query Frame = 0


Query: 8  EPIKDINDP-EVQQ-IGNLTVDDHNKKTGEHLKFVRVVNGL-SAQFVDPFSPLGGTLYEL 67
          +PI+DIND  +VQ  IG L V+DHNKKTG++L+ V VVNGL S  FV P S   G LY L
Sbjct: 20 KPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPGSTHEGILYHL 79

Query: 68 VLEAINAGKINWTY 79
          ++EA     INWTY
Sbjct: 80 LVEAKTIEGINWTY 93

[Close]

BLAST of Chy8G149490 vs. ExPASy TrEMBL
Match: A0A0A0L191 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE=4 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.2e-09
Identity = 43/75 (57.33%), Postives = 52/75 (69.33%), Query Frame = 0


Query: 7  LEPIKDINDPEVQQIGNLTVDDHNK-KTGEHLKFVRVVNGL-SAQFVDP-FSPLGGTLYE 66
          L+PI DINDP VQ IG L V+DH+    GE LKFVRVVNGL S +++ P F+ +   LY 
Sbjct: 10 LKPIIDINDPHVQIIGKLAVEDHDSHNPGEKLKFVRVVNGLKSDKYIGPGFTEV--ILYH 69

Query: 67 LVLEAINAGKINWTY 79
          LVLEA    +INWTY
Sbjct: 70 LVLEAKTNEEINWTY 82

[Close]

BLAST of Chy8G149490 vs. NCBI nr
Match: KAE8649716.1 (hypothetical protein Csa_012476 [Cucumis sativus])

HSP 1 Score: 147 bits (371), Expect = 6.47e-44
Identity = 70/79 (88.61%), Postives = 74/79 (93.67%), Query Frame = 0


Query: 1  MSIMSPLEPIKDINDPEVQQIGNLTVDDHNKKTGEHLKFVRVVNGLSAQFVDPFSPLGGT 60
          MS  SP EPIKDINDP+VQ+IGNLTVDDHNKKTGE+LKFVRVVNGL A FVDPFSPLGGT
Sbjct: 1  MSFTSPFEPIKDINDPQVQEIGNLTVDDHNKKTGENLKFVRVVNGLFAGFVDPFSPLGGT 60

Query: 61 LYELVLEAINAGKINWTYK 79
          +YELVLEAINAGKINWTYK
Sbjct: 61 VYELVLEAINAGKINWTYK 79

[Close]

BLAST of Chy8G149490 vs. NCBI nr
Match: XP_008442277.1 (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa] >TYK05422.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa])

HSP 1 Score: 127 bits (318), Expect = 8.23e-36
Identity = 63/80 (78.75%), Postives = 68/80 (85.00%), Query Frame = 0


Query: 1  MSIMSPLEPIKDINDP-EVQQIGNLTVDDHNKKTGEHLKFVRVVNGLSAQFVDPFSPLGG 60
          MSIMS  +PI+DIND  EVQ +GNLTVDDHNKKTGE+LKFVRVVNGL+A F DP SPLGG
Sbjct: 1  MSIMSAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG 60

Query: 61 TLYELVLEAINAGKINWTYK 79
           LYELVLEAI   KINWTYK
Sbjct: 61 VLYELVLEAITTSKINWTYK 80

[Close]

BLAST of Chy8G149490 vs. NCBI nr
Match: KAE8649717.1 (hypothetical protein Csa_012134, partial [Cucumis sativus])

HSP 1 Score: 105 bits (263), Expect = 4.21e-28
Identity = 49/56 (87.50%), Postives = 55/56 (98.21%), Query Frame = 0


Query: 12 DINDPEVQQIGNLTVDDHNKKTGEHLKFVRVVNGLSAQFVDPFSPLGGTLYELVLE 67
          DINDP+VQ+IGNLTVDDHNKKTGE+LKFVRVVNGL A+FVDPFSPLGGT+YELVL+
Sbjct: 1  DINDPQVQEIGNLTVDDHNKKTGENLKFVRVVNGLFARFVDPFSPLGGTVYELVLD 56

[Close]

BLAST of Chy8G149490 vs. NCBI nr
Match: TYK05428.1 (Proteinase inhibitor I25, cystatin [Cucumis melo var. makuwa])

HSP 1 Score: 71.6 bits (174), Expect = 6.04e-14
Identity = 41/74 (55.41%), Postives = 52/74 (70.27%), Query Frame = 0


Query: 8  EPIKDINDP-EVQQI-GNLTVDDHNKKTGEHLKFVRVVNGLSAQ-FVDPFSPLGGTLYEL 67
          +PI++IND  +VQ I G L V+DHNKKTGE+L+FV VVNGL ++ FV P     G LY L
Sbjct: 11 KPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGLRSEVFVQPGCTHEGILYHL 70

Query: 68 VLEAINAGKINWTY 78
          ++EA     INWTY
Sbjct: 71 LVEAKTIEGINWTY 84

[Close]

BLAST of Chy8G149490 vs. NCBI nr
Match: XP_031739698.1 (uncharacterized protein LOC116403239 [Cucumis sativus] >KAE8649718.1 hypothetical protein Csa_012480 [Cucumis sativus])

HSP 1 Score: 71.6 bits (174), Expect = 1.01e-13
Identity = 42/74 (56.76%), Postives = 51/74 (68.92%), Query Frame = 0


Query: 8  EPIKDINDP-EVQQI-GNLTVDDHNKKTGEHLKFVRVVNGL-SAQFVDPFSPLGGTLYEL 67
          +PI+DIND  +VQ I G L V+DHNKKTG++L+ V VVNGL S  FV P S   G LY L
Sbjct: 20 KPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPGSTHEGILYHL 79

Query: 68 VLEAINAGKINWTY 78
          ++EA     INWTY
Sbjct: 80 LVEAKTIEGINWTY 93

[Close]

BLAST of Chy8G149490 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 8.2e-06
Identity = 22/65 (33.85%), Postives = 39/65 (60.00%), Query Frame = 0


Query: 8  EPIKDINDPEVQQIGNLTVDDHNKKTGEHLKFVRVVNGLSAQFVDPFSPLGGTLYELVLE 67
          +PIK+++DP+V  +    +++HNK++ E L FV+VV G +         + GT Y+L + 
Sbjct: 33 KPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTT-------QVVSGTKYDLKIA 90

Query: 68 AINAG 73
          A + G
Sbjct: 93 AKDGG 90

[Close]

BLAST of Chy8G149490 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 41.6 bits (96), Expect = 3.5e-04
Identity = 22/70 (31.43%), Postives = 37/70 (52.86%), Query Frame = 0


Query: 9  PIKDINDPEVQQIGNLTVDDHNKKTGEHLKFVRVVNGLSAQFVDPFSPLGGTLYELVLEA 68
          PI ++ DP+V +IG   V ++NK++   LKF  VV+G +         + GT Y L + A
Sbjct: 33 PISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGET-------QVVSGTNYRLKVAA 92

Query: 69 INAGKINWTY 79
           +   ++  Y
Sbjct: 93 NDGDGVSKNY 95

The following BLAST results are available for this feature:

BLAST of Chy8G149490 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84WT8	1.2e-04	33.85	Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=...	[more]
P37842	7.5e-04	41.67	Multicystatin OS=Solanum tuberosum OX=4113 PE=1 SV=1	[more]

BLAST of Chy8G149490 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TJK0	1.1e-26	78.75	Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3B5B9	1.1e-26	78.75	cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=...	[more]
A0A5D3C2L1	5.6e-10	55.41	Proteinase inhibitor I25, cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A0A0L3W1	5.6e-10	56.76	Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE...	[more]
A0A0A0L191	1.2e-09	57.33	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE...	[more]

BLAST of Chy8G149490 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8649716.1	6.47e-44	88.61	hypothetical protein Csa_012476 [Cucumis sativus]	[more]
XP_008442277.1	8.23e-36	78.75	PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cys...	[more]
KAE8649717.1	4.21e-28	87.50	hypothetical protein Csa_012134, partial [Cucumis sativus]	[more]
TYK05428.1	6.04e-14	55.41	Proteinase inhibitor I25, cystatin [Cucumis melo var. makuwa]	[more]
XP_031739698.1	1.01e-13	56.76	uncharacterized protein LOC116403239 [Cucumis sativus] >KAE8649718.1 hypothetica...	[more]

BLAST of Chy8G149490 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16500.1	8.2e-06	33.85	Cystatin/monellin superfamily protein	[more]
AT5G47550.1	3.5e-04	31.43	Cystatin/monellin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 10..78 e-value: 2.5E-11 score: 43.7
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 11..68 e-value: 1.39436E-7 score: 42.8464
None	No IPR available	GENE3D	3.10.450.10		coord: 2..79 e-value: 9.1E-15 score: 56.5
None	No IPR available	PANTHER	PTHR47116:SF5	PHLOEM FILAMENT PROTEIN	coord: 7..78
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 9..71
IPR027214	Cystatin	PANTHER	PTHR47116	PHLOEM FILAMENT PROTEIN	coord: 7..78

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy8G149490.1	Chy8G149490.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity