Chy8G148920 (gene) Cucumber (hystrix) v1

Overview


Name	Chy8G148920
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
Location	chrH08: 6698955 .. 6700189 (-)
RNA-Seq Expression	Chy8G148920
Synteny	Chy8G148920

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGAAAAGTACGAACCATCCCAACAGGAACAAGATCATGCTTTTTTGTTGCAGCTTCTTTTCTGGTTCAAACAGTCATTCAAGTACTTGCCTTGTATAACTTTATTGGATAGATTATTAAACTCCAATCATTTTCGGACTCCATGGTATTCTTTTAAGTTGTAATCCTTGTTCAATATGTTTCGATAGTTGATAGTTCTTACTGATATGATTTGAATCTTGTGATGTATTTAGTCCCCTTATGTGTTATGCTAACATAATTGGTCGATTAAACTCTTGCTTTTATTTATTATTGTGAAGTTGGGTTAATGCACCTTCTTGTGAGCACTGTGGAGATACAACAACATTTCAAGATATGGACGATCCACTTCCTTCAAAACTTCAATTTGGTGGTTCCCGAGTTGAATTATATTGGCATGTGCAATGGACTCCTTTGGTTAGACTTATCTTCTAGATTTGACATTCTATATTGCTGGAACTCTTCTCTGTTATGTTCATGTATAGTATATTTTTTTCCAAATTACATAAAGAATAAGATCTAAACGAATTGTTTATAATTTTTTCCTGCTACCTTTGTGATTACTTTCTCTACCATCTAACGTTCCGCCTATATTATTTGATTGAATGATGTGTTATGTGACATTTTTTATTACCATCATGTTATTCTTGTACAACTACTAATAGTTTTCTTCATTCAAATAAATAGCCATGAAGATGAATTTGGTTTATTTTATTTCTTACATTTCTTCTTAAAATATACATTTTGTGTTTTTAAATCAAATAAATAATTATTATATATATAAAAAAAAGAATCCCAACTTGGTACTAACCAAGCGTGGAAAACGTAACACTTACTATTATTATTGTAGTTTAACTTATTTAATTACAAATGCAAAAGAACTTTTTTTGTACACCTATACGTGTTTGGATTTTTACTCTTTTATTTCATTATCAATGAAATTTGTTTTTCTTTTCTAGAAAGATGGTCTGAAATGGGTTCCATTTCCAAATGTGAATAGTTTTTAGACAGCGATAAATATTAGGCGTAGGCATAGAGTTTTAGTTTGAGGTTTTGGTGTCTTAGTGAATGGTAGAAATAAAATAAATGTATTTCATATACTTCCCTCATTCCTCTCTAATCTTCTACTTCTCTCTCTTCTCTCTCTTTTTATACTTCTAGCTGTAAGTCATGCCAAAAGGTTACTCATTTCCCACGTTTCAACGATCCAATAAAG

mRNA sequence

GAGGAAAAGTACGAACCATCCCAACAGGAACAAGATCATGCTTTTTTGTTGCAGCTTCTTTTCTGGTTCAAACAGTCATTCAATTGGGTTAATGCACCTTCTTGTGAGCACTGTGGAGATACAACAACATTTCAAGATATGGACGATCCACTTCCTTCAAAACTTCAATTTGGTGGTTCCCGAGTTGAATTATATTGGCATGTGCAATGGACTCCTTTGCTGTAAGTCATGCCAAAAGGTTACTCATTTCCCACGTTTCAACGATCCAATAAAG

Coding sequence (CDS)

GAGGAAAAGTACGAACCATCCCAACAGGAACAAGATCATGCTTTTTTGTTGCAGCTTCTTTTCTGGTTCAAACAGTCATTCAATTGGGTTAATGCACCTTCTTGTGAGCACTGTGGAGATACAACAACATTTCAAGATATGGACGATCCACTTCCTTCAAAACTTCAATTTGGTGGTTCCCGAGTTGAATTATATTGGCATGTGCAATGGACTCCTTTGCTGTAAGTCATGCCAAAAGGTTACTCATTTCCCACGTTTCAACGATCCAATAAAG

Protein sequence

EEKYEPSQQEQDHAFLLQLLFWFKQSFNWVNAPSCEHCGDTTTFQDMDDPLPSKLQFGGSRVELYWHVQWTPLL*VMPKGYSFPTFQRSNKX

Homology

[Close]

BLAST of Chy8G148920 vs. ExPASy Swiss-Prot
Match: Q9FGY9 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Arabidopsis thaliana OX=3702 GN=PNG1 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 8.2e-18
Identity = 38/65 (58.46%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 1   EEKYEPSQQEQDHAFLLQLLFWFKQSFNWVNAPSCEHCGDTTTFQDMDDPLPSKLQFGGS 60
           E  +EPS++E+D+AFLLQLLFWFK+SF WVN P C+ CG+ T  Q M +PL S+L +G +
Sbjct: 159 EGNFEPSKEERDYAFLLQLLFWFKKSFRWVNEPPCDFCGNKTIGQGMGNPLTSELAYGAN 218

Query: 61  RVELY 66
           RVE+Y
Sbjct: 219 RVEIY 223

[Close]

BLAST of Chy8G148920 vs. ExPASy Swiss-Prot
Match: Q7F0R1 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Oryza sativa subsp. japonica OX=39947 GN=PNG1 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.1e-17
Identity = 39/65 (60.00%), Postives = 48/65 (73.85%), Query Frame = 0


Query: 1   EEKYEPSQQEQDHAFLLQLLFWFKQSFNWVNAPSCEHCGDTTTFQDMDDPLPSKLQFGGS 60
           E  + PS+ E+DHAFLLQLLFWFKQSF WVNAP C+ CG  T    M   LPS+++FG +
Sbjct: 175 EGNFSPSKDEEDHAFLLQLLFWFKQSFRWVNAPPCDSCGRETFNVGMGTALPSEIKFGAN 234

Query: 61  RVELY 66
           RVE+Y
Sbjct: 235 RVEIY 239

[Close]

BLAST of Chy8G148920 vs. ExPASy TrEMBL
Match: A0A1S3C348 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucumis melo OX=3656 GN=LOC103495944 PE=4 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.6e-27
Identity = 58/65 (89.23%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 1   EEKYEPSQQEQDHAFLLQLLFWFKQSFNWVNAPSCEHCGDTTTFQDMDDPLPSKLQFGGS 60
           E KYEPS+QEQDHAFLLQLLFWFKQSF WVNAPSCEHCGDT+TFQDM DPLPS+LQFGG 
Sbjct: 50  EGKYEPSKQEQDHAFLLQLLFWFKQSFRWVNAPSCEHCGDTSTFQDMGDPLPSELQFGGY 109

Query: 61  RVELY 66
           RVELY
Sbjct: 110 RVELY 114

[Close]

BLAST of Chy8G148920 vs. ExPASy TrEMBL
Match: A0A5D3D4F2 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001350 PE=4 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.6e-27
Identity = 58/65 (89.23%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 1   EEKYEPSQQEQDHAFLLQLLFWFKQSFNWVNAPSCEHCGDTTTFQDMDDPLPSKLQFGGS 60
           E KYEPS+QEQDHAFLLQLLFWFKQSF WVNAPSCEHCGDT+TFQDM DPLPS+LQFGG 
Sbjct: 44  EGKYEPSKQEQDHAFLLQLLFWFKQSFRWVNAPSCEHCGDTSTFQDMGDPLPSELQFGGY 103

Query: 61  RVELY 66
           RVELY
Sbjct: 104 RVELY 108

[Close]

BLAST of Chy8G148920 vs. ExPASy TrEMBL
Match: A0A5A7VB18 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold329G001280 PE=4 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.6e-27
Identity = 58/65 (89.23%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 1   EEKYEPSQQEQDHAFLLQLLFWFKQSFNWVNAPSCEHCGDTTTFQDMDDPLPSKLQFGGS 60
           E KYEPS+QEQDHAFLLQLLFWFKQSF WVNAPSCEHCGDT+TFQDM DPLPS+LQFGG 
Sbjct: 50  EGKYEPSKQEQDHAFLLQLLFWFKQSFRWVNAPSCEHCGDTSTFQDMGDPLPSELQFGGY 109

Query: 61  RVELY 66
           RVELY
Sbjct: 110 RVELY 114

[Close]

BLAST of Chy8G148920 vs. ExPASy TrEMBL
Match: A0A0A0LG82 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucumis sativus OX=3659 GN=Csa_2G011510 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 5.9e-27
Identity = 57/65 (87.69%), Postives = 62/65 (95.38%), Query Frame = 0


Query: 1   EEKYEPSQQEQDHAFLLQLLFWFKQSFNWVNAPSCEHCGDTTTFQDMDDPLPSKLQFGGS 60
           E KYEPS+QEQDHAFLLQLLFWFKQSF+WVNAPSCEHCGDTTT+QDM +PLPS+LQFGG 
Sbjct: 50  EGKYEPSKQEQDHAFLLQLLFWFKQSFSWVNAPSCEHCGDTTTYQDMGNPLPSELQFGGY 109

Query: 61  RVELY 66
           RVELY
Sbjct: 110 RVELY 114

[Close]

BLAST of Chy8G148920 vs. ExPASy TrEMBL
Match: A0A6J1HP96 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucurbita maxima OX=3661 GN=LOC111465478 PE=4 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 4.2e-25
Identity = 54/65 (83.08%), Postives = 59/65 (90.77%), Query Frame = 0


Query: 1   EEKYEPSQQEQDHAFLLQLLFWFKQSFNWVNAPSCEHCGDTTTFQDMDDPLPSKLQFGGS 60
           E KYEPS+ EQDHAFLLQLLFWFKQSF WVNAPSCEHCGD TTFQDM +PLPS+LQFG +
Sbjct: 51  EGKYEPSKLEQDHAFLLQLLFWFKQSFRWVNAPSCEHCGDATTFQDMGNPLPSELQFGAN 110

Query: 61  RVELY 66
           RVE+Y
Sbjct: 111 RVEVY 115

[Close]

BLAST of Chy8G148920 vs. NCBI nr
Match: XP_023522904.1 (peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 122 bits (305), Expect = 7.65e-33
Identity = 54/65 (83.08%), Postives = 58/65 (89.23%), Query Frame = 0


Query: 1   EEKYEPSQQEQDHAFLLQLLFWFKQSFNWVNAPSCEHCGDTTTFQDMDDPLPSKLQFGGS 60
           E KYEPS+ EQDHAFLLQLLFWFKQ F WVNAPSCEHCGD TTFQDM DPLPS+LQFG +
Sbjct: 60  EGKYEPSKLEQDHAFLLQLLFWFKQLFRWVNAPSCEHCGDATTFQDMGDPLPSELQFGAN 119

Query: 61  RVELY 65
           RVE+Y
Sbjct: 120 RVEVY 124

[Close]

BLAST of Chy8G148920 vs. NCBI nr
Match: TYK18410.1 (peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucumis melo var. makuwa])

HSP 1 Score: 129 bits (325), Expect = 1.03e-32
Identity = 58/65 (89.23%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 1   EEKYEPSQQEQDHAFLLQLLFWFKQSFNWVNAPSCEHCGDTTTFQDMDDPLPSKLQFGGS 60
           E KYEPS+QEQDHAFLLQLLFWFKQSF WVNAPSCEHCGDT+TFQDM DPLPS+LQFGG 
Sbjct: 44  EGKYEPSKQEQDHAFLLQLLFWFKQSFRWVNAPSCEHCGDTSTFQDMGDPLPSELQFGGY 103

Query: 61  RVELY 65
           RVELY
Sbjct: 104 RVELY 108

[Close]

BLAST of Chy8G148920 vs. NCBI nr
Match: KAA0063616.1 (peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucumis melo var. makuwa])

HSP 1 Score: 129 bits (325), Expect = 1.13e-32
Identity = 58/65 (89.23%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 1   EEKYEPSQQEQDHAFLLQLLFWFKQSFNWVNAPSCEHCGDTTTFQDMDDPLPSKLQFGGS 60
           E KYEPS+QEQDHAFLLQLLFWFKQSF WVNAPSCEHCGDT+TFQDM DPLPS+LQFGG 
Sbjct: 50  EGKYEPSKQEQDHAFLLQLLFWFKQSFRWVNAPSCEHCGDTSTFQDMGDPLPSELQFGGY 109

Query: 61  RVELY 65
           RVELY
Sbjct: 110 RVELY 114

[Close]

BLAST of Chy8G148920 vs. NCBI nr
Match: XP_008455847.1 (PREDICTED: peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucumis melo] >XP_008455857.1 PREDICTED: peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucumis melo])

HSP 1 Score: 129 bits (325), Expect = 1.18e-32
Identity = 58/65 (89.23%), Postives = 61/65 (93.85%), Query Frame = 0


Query: 1   EEKYEPSQQEQDHAFLLQLLFWFKQSFNWVNAPSCEHCGDTTTFQDMDDPLPSKLQFGGS 60
           E KYEPS+QEQDHAFLLQLLFWFKQSF WVNAPSCEHCGDT+TFQDM DPLPS+LQFGG 
Sbjct: 50  EGKYEPSKQEQDHAFLLQLLFWFKQSFRWVNAPSCEHCGDTSTFQDMGDPLPSELQFGGY 109

Query: 61  RVELY 65
           RVELY
Sbjct: 110 RVELY 114

[Close]

BLAST of Chy8G148920 vs. NCBI nr
Match: XP_011648762.1 (peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucumis sativus] >XP_011648763.1 peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucumis sativus] >XP_031737149.1 peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucumis sativus])

HSP 1 Score: 128 bits (322), Expect = 2.89e-32
Identity = 57/65 (87.69%), Postives = 62/65 (95.38%), Query Frame = 0


Query: 1   EEKYEPSQQEQDHAFLLQLLFWFKQSFNWVNAPSCEHCGDTTTFQDMDDPLPSKLQFGGS 60
           E KYEPS+QEQDHAFLLQLLFWFKQSF+WVNAPSCEHCGDTTT+QDM +PLPS+LQFGG 
Sbjct: 50  EGKYEPSKQEQDHAFLLQLLFWFKQSFSWVNAPSCEHCGDTTTYQDMGNPLPSELQFGGY 109

Query: 61  RVELY 65
           RVELY
Sbjct: 110 RVELY 114

[Close]

BLAST of Chy8G148920 vs. TAIR 10
Match: AT5G49570.1 (peptide-N-glycanase 1 )

HSP 1 Score: 90.9 bits (224), Expect = 5.8e-19
Identity = 38/65 (58.46%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 1   EEKYEPSQQEQDHAFLLQLLFWFKQSFNWVNAPSCEHCGDTTTFQDMDDPLPSKLQFGGS 60
           E  +EPS++E+D+AFLLQLLFWFK+SF WVN P C+ CG+ T  Q M +PL S+L +G +
Sbjct: 159 EGNFEPSKEERDYAFLLQLLFWFKKSFRWVNEPPCDFCGNKTIGQGMGNPLTSELAYGAN 218

Query: 61  RVELY 66
           RVE+Y
Sbjct: 219 RVEIY 223

The following BLAST results are available for this feature:

BLAST of Chy8G148920 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FGY9	8.2e-18	58.46	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Arabidopsis thali...	[more]
Q7F0R1	1.1e-17	60.00	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Oryza sativa subs...	[more]

BLAST of Chy8G148920 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C348	2.6e-27	89.23	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucumis melo OX=3...	[more]
A0A5D3D4F2	2.6e-27	89.23	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucumis melo var....	[more]
A0A5A7VB18	2.6e-27	89.23	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucumis melo var....	[more]
A0A0A0LG82	5.9e-27	87.69	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucumis sativus O...	[more]
A0A6J1HP96	4.2e-25	83.08	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucurbita maxima ...	[more]

BLAST of Chy8G148920 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023522904.1	7.65e-33	83.08	peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase-like, partial [Cucur...	[more]
TYK18410.1	1.03e-32	89.23	peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucumis melo var. m...	[more]
KAA0063616.1	1.13e-32	89.23	peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucumis melo var. m...	[more]
XP_008455847.1	1.18e-32	89.23	PREDICTED: peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucumis ...	[more]
XP_011648762.1	2.89e-32	87.69	peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucumis sativus] >X...	[more]

BLAST of Chy8G148920 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G49570.1	5.8e-19	58.46	peptide-N-glycanase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.20.25.10		coord: 31..66 e-value: 2.3E-6 score: 29.8
None	No IPR available	PANTHER	PTHR12143:SF19	PEPTIDE-N(4)-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE	coord: 2..65
None	No IPR available	PANTHER	PTHR12143	PEPTIDE N-GLYCANASE PNGASE -RELATED	coord: 2..65

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy8G148920.1	Chy8G148920.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006516	glycoprotein catabolic process
biological_process	GO:0006517	protein deglycosylation
biological_process	GO:0006515	protein quality control for misfolded or incompletely synthesized proteins
biological_process	GO:0010188	response to microbial phytotoxin
biological_process	GO:0010193	response to ozone
biological_process	GO:0009751	response to salicylic acid
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000224	peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity