Chy8G148370 (gene) Cucumber (hystrix) v1

Overview
NameChy8G148370
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionWAT1-related protein
LocationchrH08: 5223604 .. 5228006 (-)
RNA-Seq ExpressionChy8G148370
SyntenyChy8G148370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGAAAGGGAAGTGGAATTGCCAATTTTGTTGAGGGAGCACAACCTTACATTGCTATGATCTCTCTCCAATTTGGCTATGCTGGGATGAATATCATCACCAAGGTAGCTCTCAACCGCGGAATGAGCCATTACGTTCTTGTCACCTATCGGCAAGCCTTTGCCACCATTGTCCTTGCTCCCTTCGCCTTCTTCTTCGAAAGGTTGTTCTTTCTATGGCTTTTTCTCTAAATTTCTGACTGATTTTGAAATAAACGATTTTAGTTCTTTTTTAGGTACTTTCTTTATACTAATATCAATTTTCTTATCAGGAAAATGAGACCCAAGATCAGTTTCGCTATGTTAATGCAGATTTTTCTTCTGGGTTTGTTAGGGTTAGTGCAACTTTTAGATCTTTTGTAAAAAAAATAAAATTAAAAAGTGGATTTTTAACCGTTATTTTTTAGTTACAAAATAATTGTTCTTTTTTCTTCTTCAGACCAGTGATTGATCAGAATTTTTATTATGCTGGATTGAAGCTCACTTCAACTACCTTTTCATGTGCCACGAGCAATATGCTTCCTGCAATGACATTCATCTTGGCTCTTCTTTGTAGGTATACAATATTACCACTTTGCATTGAGATAAAAACTCGTAAAATATTGTTTTTAAACTTGTGTAGAATTTTCTTTTACATTTAATTTTGATTTTATGAAAATCATGTTTAAAAATGTCAAATTAAATATTAAATTGATTTAATGAAATCAATTAATTGCATGTTTATAAGTAGTTTTCAACCTAGGCAGAGCTATTTTATAACTCTTTCAAATTTACTTTTCAAACATGCTCTGCGTTGTAAATTTGTCTTTTAAGTTTTACCTTAAAGCCTTGTGTGCAAAATTGAACTTCTTGTATATGTATATTTTCACTTTTATTCAACTTTTATAAATCACTTCCAACATAGTCGAAAATTTGTTATCAATTTACTTTTAAACTTTACCTTAAAGTTTCGTATACAGAAATCAAAATTTTTGTAAACGTATATTTTCAATTTTAATCCATTTTTATAAATCACTTCCAAATATCTCCTAACAATCATTTTCAATTTGCTTTTGAACTTTACTTTAAATTTTTGTTAACGAAGTCAAATTTCTTGTATCCGTATATATATATTTGCACTTTTAAACTACTTTTAGCAATCTCTAAATTCCATTATTTACTTTTGAACTCTACTTTGAAGTTTCGTATACGAAATCAAACTTGCTCTTGCATACGTATATTTACACTATAAATCAACTGTTATGAATTCGACTCTTAATAAAATATGTACATGCATGTACCATATCAAACCCTTTTAAAAAGCAGAAGATAAAAAGCAAAACTTTCTTGTTGCTAATAATAAAAGACTTCAAAAAGAGAGAGAGCATAAAAGTATATGATTAATATTTTGAAATTAATTTATTTAAAATTGTTTGTTTAAATGAAAAAAAAATGAAAATTTGAAAGGTTGGAGAAATTGGAGATGAAGAAAGTGAGGTGCCAAGCCAAGGTGGTGGGAACATTGGTGACAGTGGGTGGAGCCATTTTGATGACTTTATACAAAGGGAATGTCATAAGCTTCTTTTGGTCTCACCATAACAATAATTATCTTCACAGCTCTTCTGCTTCTTCCAATTACTATTCCTTTGAAAGCACTTATCAAGATTGGCTCAAGGGTTCCATTCTTCTCCTTTTTGCCAATCTTGCTTGGGCTTTGTTCTTCATCGTTCAGGTACTAATATTTTTACACTAGGTTTAACTGCCTTCAACATGTTTGTTCTATTTTAAGTATTTTACTTTTTTTTTAACTCTTGAGATAAAAATAGTTTTCTTTTAATAATGTAGAATGAAGGAATTGTACCTCTTATCTCGACGTTGATAACACAAAAGTATAGGAATTTGATTAAGCATAAATATATGTGATATCACATCAAGATATATATTTTTTCAAATAATTATGACAGTTGTATACATCCCAAATTACAGTTATTTGGCGGGAAGATGTATATAATTATAGAGACTTAAAATCAAAAGGATCATTTTTAGGTTTAGTTATTAAATTTTTAAAGTCGTATATCTTTTTGGTTTTGAACTTAAAAAATGTCTAATAGGACTTCCAATTTTCAATTGATCCCTAAACTTCCAATTTTCAATTGATTCCTAGACTTCCAATTTTATGTTTAATAATTGGTCTCGATCGAATTTTCAATTTTATATCTAATTCAATTTCAATTTTATATCTAATTGAAGAGATAATTGAGAGTGAATAAAATATGTCATTTGTCTTTTATTCAATTTTGTTTTTCAATGTATAGACCTAATAGACAAAAGGAAAAGGTTCTCTTTAAACAAAAAATATTCAATTATATACATACATAGGTCATTTAATTTTTTTGAAAAAGAATATTTAATACGTGATCTATTAAATATAATATTGAAAGCTTACAACATATTCTATTAGATATCTCTAAAGATTATCAATCTATTAGACACAAAAAATCAAACATTTTTAAAGACTTATACAAACTTGAAAGTTTAGAAACTAAACTTATAATTTTACCTTTACAATATAACAGTAGTCAATCGGATCGAATTGATATAGTTCATATTACATGTGTATGGTTTTACTATTTCACAGGCAATGACATTAAGGAATTACACAGCCCATCTGTCACTTACAACCCTTATGTGTTTCTTTGGGACTTTGCAATCCATGGCTGTCACATTTGTGATGGAACACAAAGCTTCTGTTTGGAACATTGGATGGGATATGAACCTTCTTGCTTCTGTTTATGCTGTAAGATTCTCCATCTCTTTATTTTTATATATTCCTTGAGTAATAATAATTTAATTTAATTTGAATTATGAACTTTGTTCAGGGAATAGTTTCATCAAGCATAGCTTATTATGTACAAGGGATGATTATGCAAAAGAGAGGGCCAGTTTTTGTCACAGCCTTTACCCCTATGATCATGATCATTGTTGCCATTATGGGCTCCTTCATGCTTGCTGAGAAGATCTATATCGGACGGTTAGTACCATCACTTAACCTAAACGTTTAAATTGTTAATCAATGATTTTCATTTTGTTTATATTTTTACTTTGTCCCTTTTACTTGTAAGTTTAGAAATTAGCGCCATACGCTACAAAGTCAATCTTTAAAATGATATAAGTGTTATTGAAATCAGTAGTAATTGAAGTAACAAAATATTCAAGTTTATAAAGTTAGGAAAAATTCACAACAAAAAATCTTTTAATTTTATCCTGTTAACACTGTTATTTGAATTTTGTATTTTCTTTCTTTTTTTTACACAGTTGTAGGAAAATAATTAAAAATAAAAGTATGAATTGAAACTAACTTTTTCTAAATGTTTCAAGACTAAAATAGATATTTTTAATATTTTAGAGCAAAATAATTAAAAATATACTAAAGTGATTATTTTAAAAGTTTAAGGAAAAAAATAAAGTGAAGATAAAAGTACAAATAGGATCTAAATTAAACCAAAGATTTAAAAGATAAGTGAAGAGTAGGTTTTTTCCTTTTTAATCTTTATAATCATTTTAATATGTAATTCTCATTTTTCATGAAATATGATATTTTCGCTCCATCATCCATAGTTTTGAATTTAAGAAGTATCTCAGGTACGGTCTAATAATAATGGATTAAATTATTCAATTATGAGCAATGATTAGGTTCATGTTCAACTTACGGTACCCAGTAAGGTTAATAATAATATTAATAACTAAAACTATAAATAATTTATTGTCCTGAAACAAATAATAATAATAATAATTTAGTCTTTTTTTCAATTTAGAAAAATAAAGTGGTACATAGGAAGAATTTATTACCAAACTCTTAATTACCCGATTCATTCATATACTGAAAGTTTTACTTTCATGCCATGTTACGTTGAATGTAATTAAACATATCAAACCTAAAAATTTATACTCTAACATACCTTAAATTGAATCTTTATACTCTTTTAACTTTATGTTCTTTTTCTTTTTTCAGTGTTGTTGGAGGTATTGTAATGGTGGTGGGGCTGTACTCAGTGCTATGGGGAAAATACAAAGATTACAAAGAAAAGGAAGCAATAATAGAAGAGACCACCATAGTTGAACCAGTGAAGCTTTTAATAAGTGAAGACAAATTGGAGAAAAATAAGAAGAAGAGATTAGCAACAGTAGTTGAAAAAGAAGAAGAAGAAGAAGAAACGACAGCATCGACATCGTTAAACGACATCGAAATGCAAAGGAACGAAACAACATCAAATGTCGACGACAATAATAACAATAATGTTGCAACATTGCGTTGTCCTTCTCCACTACCCATTGTTGTTGTTATAGCTATGAATGAAGCACCACCAAAAGTGTTTTATTAA

mRNA sequence

ATGGAAGGAAAGGGAAGTGGAATTGCCAATTTTGTTGAGGGAGCACAACCTTACATTGCTATGATCTCTCTCCAATTTGGCTATGCTGGGATGAATATCATCACCAAGGTAGCTCTCAACCGCGGAATGAGCCATTACGTTCTTGTCACCTATCGGCAAGCCTTTGCCACCATTGTCCTTGCTCCCTTCGCCTTCTTCTTCGAAAGGAAAATGAGACCCAAGATCAGTTTCGCTATGTTAATGCAGATTTTTCTTCTGGGTTTGTTAGGACCAGTGATTGATCAGAATTTTTATTATGCTGGATTGAAGCTCACTTCAACTACCTTTTCATGTGCCACGAGCAATATGCTTCCTGCAATGACATTCATCTTGGCTCTTCTTTGTAGGTTGGAGAAATTGGAGATGAAGAAAGTGAGGTGCCAAGCCAAGGTGGTGGGAACATTGGTGACAGTGGGTGGAGCCATTTTGATGACTTTATACAAAGGGAATGTCATAAGCTTCTTTTGGTCTCACCATAACAATAATTATCTTCACAGCTCTTCTGCTTCTTCCAATTACTATTCCTTTGAAAGCACTTATCAAGATTGGCTCAAGGGTTCCATTCTTCTCCTTTTTGCCAATCTTGCTTGGGCTTTGTTCTTCATCGTTCAGGCAATGACATTAAGGAATTACACAGCCCATCTGTCACTTACAACCCTTATGTGTTTCTTTGGGACTTTGCAATCCATGGCTGTCACATTTGTGATGGAACACAAAGCTTCTGTTTGGAACATTGGATGGGATATGAACCTTCTTGCTTCTGTTTATGCTGGAATAGTTTCATCAAGCATAGCTTATTATGTACAAGGGATGATTATGCAAAAGAGAGGGCCAGTTTTTGTCACAGCCTTTACCCCTATGATCATGATCATTGTTGCCATTATGGGCTCCTTCATGCTTGCTGAGAAGATCTATATCGGACGTGTTGTTGGAGGTATTGTAATGGTGGTGGGGCTGTACTCAGTGCTATGGGGAAAATACAAAGATTACAAAGAAAAGGAAGCAATAATAGAAGAGACCACCATAGTTGAACCAGTGAAGCTTTTAATAAGTGAAGACAAATTGGAGAAAAATAAGAAGAAGAGATTAGCAACAGTAGTTGAAAAAGAAGAAGAAGAAGAAGAAACGACAGCATCGACATCGTTAAACGACATCGAAATGCAAAGGAACGAAACAACATCAAATGTCGACGACAATAATAACAATAATGTTGCAACATTGCGTTGTCCTTCTCCACTACCCATTGTTGTTGTTATAGCTATGAATGAAGCACCACCAAAAGTGTTTTATTAA

Coding sequence (CDS)

ATGGAAGGAAAGGGAAGTGGAATTGCCAATTTTGTTGAGGGAGCACAACCTTACATTGCTATGATCTCTCTCCAATTTGGCTATGCTGGGATGAATATCATCACCAAGGTAGCTCTCAACCGCGGAATGAGCCATTACGTTCTTGTCACCTATCGGCAAGCCTTTGCCACCATTGTCCTTGCTCCCTTCGCCTTCTTCTTCGAAAGGAAAATGAGACCCAAGATCAGTTTCGCTATGTTAATGCAGATTTTTCTTCTGGGTTTGTTAGGACCAGTGATTGATCAGAATTTTTATTATGCTGGATTGAAGCTCACTTCAACTACCTTTTCATGTGCCACGAGCAATATGCTTCCTGCAATGACATTCATCTTGGCTCTTCTTTGTAGGTTGGAGAAATTGGAGATGAAGAAAGTGAGGTGCCAAGCCAAGGTGGTGGGAACATTGGTGACAGTGGGTGGAGCCATTTTGATGACTTTATACAAAGGGAATGTCATAAGCTTCTTTTGGTCTCACCATAACAATAATTATCTTCACAGCTCTTCTGCTTCTTCCAATTACTATTCCTTTGAAAGCACTTATCAAGATTGGCTCAAGGGTTCCATTCTTCTCCTTTTTGCCAATCTTGCTTGGGCTTTGTTCTTCATCGTTCAGGCAATGACATTAAGGAATTACACAGCCCATCTGTCACTTACAACCCTTATGTGTTTCTTTGGGACTTTGCAATCCATGGCTGTCACATTTGTGATGGAACACAAAGCTTCTGTTTGGAACATTGGATGGGATATGAACCTTCTTGCTTCTGTTTATGCTGGAATAGTTTCATCAAGCATAGCTTATTATGTACAAGGGATGATTATGCAAAAGAGAGGGCCAGTTTTTGTCACAGCCTTTACCCCTATGATCATGATCATTGTTGCCATTATGGGCTCCTTCATGCTTGCTGAGAAGATCTATATCGGACGTGTTGTTGGAGGTATTGTAATGGTGGTGGGGCTGTACTCAGTGCTATGGGGAAAATACAAAGATTACAAAGAAAAGGAAGCAATAATAGAAGAGACCACCATAGTTGAACCAGTGAAGCTTTTAATAAGTGAAGACAAATTGGAGAAAAATAAGAAGAAGAGATTAGCAACAGTAGTTGAAAAAGAAGAAGAAGAAGAAGAAACGACAGCATCGACATCGTTAAACGACATCGAAATGCAAAGGAACGAAACAACATCAAATGTCGACGACAATAATAACAATAATGTTGCAACATTGCGTTGTCCTTCTCCACTACCCATTGTTGTTGTTATAGCTATGAATGAAGCACCACCAAAAGTGTTTTATTAA

Protein sequence

MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVLAPFAFFFERKMRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAMTFILALLCRLEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSSSASSNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGTLQSMAVTFVMEHKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPMIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPVKLLISEDKLEKNKKKRLATVVEKEEEEEETTASTSLNDIEMQRNETTSNVDDNNNNNVATLRCPSPLPIVVVIAMNEAPPKVFY*
Homology
BLAST of Chy8G148370 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 6.5e-121
Identity = 229/383 (59.79%), Postives = 299/383 (78.07%), Query Frame = 0

Query: 6   SGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVLAPFAF 65
           S   +F+  ++PY AMISLQFGYAGMNIITK++LN GMSHYVLV YR A AT V+APFAF
Sbjct: 7   SSCESFLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAF 66

Query: 66  FFERKMRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAMTFILA 125
           FFERK +PKI+F++ MQ+F+LGLLGPVIDQNFYY GLK TS TFSCA SNMLPAMTFILA
Sbjct: 67  FFERKAQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILA 126

Query: 126 LLCRLEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLH---SSSA 185
           +L R+E L++KK+ CQAK+ GT+VTV GA+LMT+YKG ++  FW+     Y+H   SS A
Sbjct: 127 VLFRMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWT----KYMHIQDSSHA 186

Query: 186 SSNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAH-LSLTTLMCFFGTLQ 245
           ++      S+ +++LKGSILL+FA LAWA  F++QA  L+ Y  H LSLTTL+CF GTLQ
Sbjct: 187 NTTSSKNSSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQ 246

Query: 246 SMAVTFVMEHKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPMI 305
           ++AVTFVMEH  S W IGWDMNLLA+ Y+GIV+SSI+YYVQG++M+KRGPVF TAF+P++
Sbjct: 247 AVAVTFVMEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLM 306

Query: 306 MIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYK----EKEAIIEETTIVE 365
           M+IVA+MGSF+LAEKI++G V+G +++V+GLY+VLWGK K+ +    E   I   + + E
Sbjct: 307 MVIVAVMGSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTE 366

Query: 366 PVKLLISEDKLEKNKKKRLATVV 381
            V+   S+ K+ +     L+T+V
Sbjct: 367 DVEANGSKMKISEGDNSMLSTIV 385

BLAST of Chy8G148370 vs. ExPASy Swiss-Prot
Match: F4IJ08 (WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 8.6e-105
Identity = 215/382 (56.28%), Postives = 280/382 (73.30%), Query Frame = 0

Query: 13  EGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVLAPFAFFFERKMR 72
           E A+PY AM+ LQFGYAGMN++TK  L+RGMSHYVLV YR AFAT  +APFA   ERK+R
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 73  PKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAMTFILALLCRLEK 132
            K++F + M+IFLL LLGPVIDQN YY GLKLTS TFS A SN++PA+T ILA L R+EK
Sbjct: 67  SKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEK 126

Query: 133 LEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSSSASSNYYSFEST 192
           +EM+KVRC  KV+GTLVTV G+ILM  YKG  I+FF S       H ++ASS        
Sbjct: 127 VEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFFRS-------HLTAASS------PP 186

Query: 193 YQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGTLQSMAVTFVMEHK 252
             D+LK ++ LL A+L+WA FF++QA TL+ Y+AHLS++T++CF GTLQS+A+ FVMEH 
Sbjct: 187 TADYLKAAVFLLLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHN 246

Query: 253 ASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPMIMIIVAIMGSFM 312
            S  NIG+DMNLLAS YAGI+SSSIAYYVQG++MQ++GPVFVTAF P+I++IV+IM  F+
Sbjct: 247 PSALNIGFDMNLLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFV 306

Query: 313 LAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPVKLLISEDKLEKNK 372
           L + IY+G V+G +V++VG+Y+VLWGK+ D   +E   E+  +   VK     + L    
Sbjct: 307 LGQGIYLGGVIGVVVLMVGVYAVLWGKHVDDDGEETRHEDNVVA--VKCCSGNNGLTIMP 366

Query: 373 KKRLATVVEKEEEEEETTASTS 395
           K     + E +EE+ ET  +TS
Sbjct: 367 K-----IDEADEEDVETGKATS 368

BLAST of Chy8G148370 vs. ExPASy Swiss-Prot
Match: Q9LXX8 (WAT1-related protein At3g56620 OS=Arabidopsis thaliana OX=3702 GN=At3g56620 PE=2 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 7.5e-101
Identity = 197/348 (56.61%), Postives = 264/348 (75.86%), Query Frame = 0

Query: 13  EGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVLAPFAFFFERKMR 72
           E A+PY AM+ LQFGYAGMN++TKV L+RGMSHYVLV YR AFAT  +APFA   ERK+R
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKVVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 73  PKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAMTFILALLCRLEK 132
           PK++F + MQIF+L LLGP+IDQN YYAGLKLTS TF+ A +N++PA+TFI++++CR+EK
Sbjct: 67  PKMTFPIFMQIFVLALLGPLIDQNLYYAGLKLTSPTFAGAVTNIVPALTFIISIICRMEK 126

Query: 133 LEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSSSASSNYYSFEST 192
           +EM+KVR QAKVVGTLV V GA+LM L+K  +I+F  SH   + L  +            
Sbjct: 127 VEMRKVRFQAKVVGTLVIVVGAMLMILFKIPLITFLRSHLTGHALSPAG----------- 186

Query: 193 YQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGTLQSMAVTFVMEHK 252
            +D+LK ++ LL A+ +WA FF++QA TL+ Y++HLSL+T++CF GTLQS A+TFVME  
Sbjct: 187 -EDYLKATVFLLIASFSWASFFVLQAATLKRYSSHLSLSTMVCFMGTLQSTALTFVMEPN 246

Query: 253 ASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPMIMIIVAIMGSFM 312
            S WNIG+DMNLLAS YAGI+SSSIAYYVQGM+ +++  +FVTAF P+++II +I+G  +
Sbjct: 247 LSAWNIGFDMNLLASAYAGIMSSSIAYYVQGMMTKQKSVIFVTAFNPLVVIIGSIIGFLI 306

Query: 313 LAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPVK 361
           L + + +G V+G  ++VVG+ +VLWGK  D  E+E I  E   VE VK
Sbjct: 307 LNQTLNLGGVLGMAILVVGVCTVLWGKEGDIDEEENI--EEKFVEIVK 340

BLAST of Chy8G148370 vs. ExPASy Swiss-Prot
Match: F4HZQ7 (WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 4.7e-87
Identity = 181/399 (45.36%), Postives = 261/399 (65.41%), Query Frame = 0

Query: 5   GSGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVLAPFA 64
           G G+ N     +PY+AMIS+QFGYAGM IIT V+L  GM+HYVL  YR A AT V+APFA
Sbjct: 2   GRGLMN---SLKPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFA 61

Query: 65  FFFERKMRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAMTFIL 124
            F ERK+RPK++F + +QI LLG + PV+DQN YY G+  TS TF+ AT+N+LPA+TF+L
Sbjct: 62  LFHERKIRPKMTFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVL 121

Query: 125 ALLCRLEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFF-WSHHNNNYLHSSSAS 184
           A++ RLE +  KKVR  AKVVGT++TV GA+LMTLYKG ++ F  +          +  S
Sbjct: 122 AIIFRLESVNFKKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGS 181

Query: 185 SNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGTLQSM 244
                  +  + W+ G+++LL     WA FFI+Q+ TL+ Y A LSLTTL+C  GTL+  
Sbjct: 182 HGGAGAAAMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGT 241

Query: 245 AVTFVMEHKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPMIMI 304
           AV+ V     S W IG+D NL A+ Y+G++ S +AYYVQG++M++RGPVFV  F P+ ++
Sbjct: 242 AVSLVTVRDLSAWKIGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVV 301

Query: 305 IVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYK--EKEAIIEETTIVEPVKL 364
           I A +G  +L+E I++G V+G + ++VGLY+V+WGK KD +  + +   +   I  PVK 
Sbjct: 302 ITAALGVVVLSESIHLGSVIGTLFIIVGLYTVVWGKGKDKRMTDDDEDCKGLPIKSPVKP 361

Query: 365 LISEDKLEKNKKKRLATVVEKEEEEEETTASTSLNDIEM 401
           +        +  K LA  +E + +E +   +T+   +E+
Sbjct: 362 V--------DTGKGLAAELEMKSKEGQEAKATTTTQVEI 389

BLAST of Chy8G148370 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.6e-85
Identity = 175/353 (49.58%), Postives = 244/353 (69.12%), Query Frame = 0

Query: 16  QPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVLAPFAFFFERKMRPKI 75
           +P+I ++SLQFGYAG++II K ALN+GMS +VL +YR   ATI +APFA+F +RK+RPK+
Sbjct: 7   KPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPKM 66

Query: 76  SFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAMTFILALLCRLEKLEM 135
           + ++  +I LLGLL P IDQN YY G+K TS TF+ A +N+LPA  FI+A + RLEK+ +
Sbjct: 67  TLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNV 126

Query: 136 KKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSSSASSNYYSFESTYQD 195
           KK+  QAK++GT+VTVGGA+LMT+ KG +I   W+  N + +H  S+++         QD
Sbjct: 127 KKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWA--NPHDIHQDSSNT------GVKQD 186

Query: 196 WLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGTLQSMAVT-FVMEHKAS 255
             KG+ L+    + WA F  +QA+TL++Y   LSLT  +CF G+++S  V  F+     S
Sbjct: 187 LTKGASLIAIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPS 246

Query: 256 VWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPMIMIIVAIMGSFMLA 315
            W I  D  LLA+VY G++ S I YYVQG+IM+ RGPVFVTAF P+ M+IVAI+GS +LA
Sbjct: 247 AWAIHLDSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILA 306

Query: 316 EKIYIGRVVGGIVMVVGLYSVLWGKYKDY-------KEKEAIIEETTIVEPVK 361
           E +++GR++G IV+V+GLYSVLWGK KD         +KE  +    IV P K
Sbjct: 307 EVMFLGRILGAIVIVLGLYSVLWGKSKDEPSSSFSDMDKELPLSTPQIVLPSK 351

BLAST of Chy8G148370 vs. ExPASy TrEMBL
Match: A0A0A0KYE0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G420210 PE=3 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 3.2e-219
Identity = 412/424 (97.17%), Postives = 418/424 (98.58%), Query Frame = 0

Query: 21  MISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVLAPFAFFFERKMRPKISFAML 80
           MISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVLAPFAFFFERK+RPKISFAML
Sbjct: 1   MISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVLAPFAFFFERKVRPKISFAML 60

Query: 81  MQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAMTFILALLCRLEKLEMKKVRC 140
           MQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAMTFILALLCR+EKLEMKKVRC
Sbjct: 61  MQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAMTFILALLCRMEKLEMKKVRC 120

Query: 141 QAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSSSASSNYYSFESTYQDWLKGS 200
           QAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSSSASSNYYSFESTYQDWLKGS
Sbjct: 121 QAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSSSASSNYYSFESTYQDWLKGS 180

Query: 201 ILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGTLQSMAVTFVMEHKASVWNIGW 260
           ILLLFANLAWALFFIVQAMTLRNYTAHLSLTTL+CFFGTLQSMAVTFVMEHKASVWNIGW
Sbjct: 181 ILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLVCFFGTLQSMAVTFVMEHKASVWNIGW 240

Query: 261 DMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPMIMIIVAIMGSFMLAEKIYIG 320
           DMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPMIMIIVAIMGSFMLAEKIYIG
Sbjct: 241 DMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPMIMIIVAIMGSFMLAEKIYIG 300

Query: 321 RVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEE-TTIVEPVKLLISEDKLEKNKKKRLATV 380
           RVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEE TTIVEPVKLLISEDKLEKNKKK+LATV
Sbjct: 301 RVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEEITTIVEPVKLLISEDKLEKNKKKKLATV 360

Query: 381 VEKEEEEEETTASTSLNDIEMQRNETTSNVDDNNNNNVATLRCPSPLPIVVVIAMNEAPP 440
           V   EEEEETT STSLNDIEMQRN+T SNVDDNN+NNVATLRCPSPLPIVVVIAMNEAPP
Sbjct: 361 V---EEEEETTTSTSLNDIEMQRNDTRSNVDDNNHNNVATLRCPSPLPIVVVIAMNEAPP 420

Query: 441 KVFY 444
           KVFY
Sbjct: 421 KVFY 421

BLAST of Chy8G148370 vs. ExPASy TrEMBL
Match: A0A5A7SM95 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold34G001000 PE=3 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 5.6e-216
Identity = 410/442 (92.76%), Postives = 424/442 (95.93%), Query Frame = 0

Query: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVL 60
           MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNI+TKVALNRGMSHYVLVTYRQAFATI L
Sbjct: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIVTKVALNRGMSHYVLVTYRQAFATIAL 60

Query: 61  APFAFFFERKMRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120
           APFAFF ERK+RPKISF MLMQIFLLG LGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM
Sbjct: 61  APFAFFLERKVRPKISFTMLMQIFLLGFLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120

Query: 121 TFILALLCRLEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNY-LHS 180
           TFILALLCR+EKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNY L S
Sbjct: 121 TFILALLCRMEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYDLQS 180

Query: 181 SSASSNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGT 240
           SSASSNYYSFEST QDWLKGSILLLFANLAWALFFI+QA+TLRNYTAHLSLTTL+CFFGT
Sbjct: 181 SSASSNYYSFESTNQDWLKGSILLLFANLAWALFFIIQAITLRNYTAHLSLTTLVCFFGT 240

Query: 241 LQSMAVTFVMEHKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTP 300
           LQSMAVTFVMEH+ SVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIM+KRGPVFVTAFTP
Sbjct: 241 LQSMAVTFVMEHQTSVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMRKRGPVFVTAFTP 300

Query: 301 MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV 360
           MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV
Sbjct: 301 MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV 360

Query: 361 KLLISEDKLEKNKKKRLATVV-EKEEEEEETTASTSLNDIEMQRNETTSNVDDNNNNNVA 420
           KLLIS DK EKNKKK+LATVV E+EEEEEETT ST+LNDIEMQRN+TTSNVDD NNNNVA
Sbjct: 361 KLLISVDKFEKNKKKKLATVVEEEEEEEEETTTSTALNDIEMQRNDTTSNVDD-NNNNVA 420

Query: 421 TLRCPSPLPIVVVIAMNEAPPK 441
            L   SPLPI+VVIAM+EA PK
Sbjct: 421 KLPRLSPLPILVVIAMDEATPK 441

BLAST of Chy8G148370 vs. ExPASy TrEMBL
Match: A0A1S3C166 (WAT1-related protein At5g07050-like OS=Cucumis melo OX=3656 GN=LOC103495845 PE=3 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 5.6e-216
Identity = 410/442 (92.76%), Postives = 424/442 (95.93%), Query Frame = 0

Query: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVL 60
           MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNI+TKVALNRGMSHYVLVTYRQAFATI L
Sbjct: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIVTKVALNRGMSHYVLVTYRQAFATIAL 60

Query: 61  APFAFFFERKMRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120
           APFAFF ERK+RPKISF MLMQIFLLG LGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM
Sbjct: 61  APFAFFLERKVRPKISFTMLMQIFLLGFLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120

Query: 121 TFILALLCRLEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNY-LHS 180
           TFILALLCR+EKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNY L S
Sbjct: 121 TFILALLCRMEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYDLQS 180

Query: 181 SSASSNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGT 240
           SSASSNYYSFEST QDWLKGSILLLFANLAWALFFI+QA+TLRNYTAHLSLTTL+CFFGT
Sbjct: 181 SSASSNYYSFESTNQDWLKGSILLLFANLAWALFFIIQAITLRNYTAHLSLTTLVCFFGT 240

Query: 241 LQSMAVTFVMEHKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTP 300
           LQSMAVTFVMEH+ SVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIM+KRGPVFVTAFTP
Sbjct: 241 LQSMAVTFVMEHQTSVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMRKRGPVFVTAFTP 300

Query: 301 MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV 360
           MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV
Sbjct: 301 MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV 360

Query: 361 KLLISEDKLEKNKKKRLATVV-EKEEEEEETTASTSLNDIEMQRNETTSNVDDNNNNNVA 420
           KLLIS DK EKNKKK+LATVV E+EEEEEETT ST+LNDIEMQRN+TTSNVDD NNNNVA
Sbjct: 361 KLLISVDKFEKNKKKKLATVVEEEEEEEEETTTSTALNDIEMQRNDTTSNVDD-NNNNVA 420

Query: 421 TLRCPSPLPIVVVIAMNEAPPK 441
            L   SPLPI+VVIAM+EA PK
Sbjct: 421 KLPRLSPLPILVVIAMDEATPK 441

BLAST of Chy8G148370 vs. ExPASy TrEMBL
Match: A0A5D3CH74 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00120 PE=3 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 2.7e-202
Identity = 392/442 (88.69%), Postives = 405/442 (91.63%), Query Frame = 0

Query: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVL 60
           MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNI+TKVALNRGMSHYVLVTYRQAFATI L
Sbjct: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIVTKVALNRGMSHYVLVTYRQAFATIAL 60

Query: 61  APFAFFFERKMRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120
           APFAFF ER                     PVIDQNFYYAGLKLTSTTFSCATSNMLPAM
Sbjct: 61  APFAFFLER---------------------PVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120

Query: 121 TFILALLCRLEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNY-LHS 180
           TFILALLCR+EKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNY L S
Sbjct: 121 TFILALLCRMEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYDLQS 180

Query: 181 SSASSNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGT 240
           SSASSNYYSFEST QDWLKGSILLLFANLAWALFFI+QA+TLRNYTAHLSLTTL+CFFGT
Sbjct: 181 SSASSNYYSFESTNQDWLKGSILLLFANLAWALFFIIQAITLRNYTAHLSLTTLVCFFGT 240

Query: 241 LQSMAVTFVMEHKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTP 300
           LQSMAVTFVMEH+ SVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIM+KRGPVFVTAFTP
Sbjct: 241 LQSMAVTFVMEHQTSVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMRKRGPVFVTAFTP 300

Query: 301 MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV 360
           MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV
Sbjct: 301 MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV 360

Query: 361 KLLISEDKLEKNKKKRLATVV-EKEEEEEETTASTSLNDIEMQRNETTSNVDDNNNNNVA 420
           KLLIS DK EKNKKK+LATVV E+EEEEEETT ST+LNDIEMQRN+TTSNVDD NNNNVA
Sbjct: 361 KLLISVDKFEKNKKKKLATVVEEEEEEEEETTTSTALNDIEMQRNDTTSNVDD-NNNNVA 420

Query: 421 TLRCPSPLPIVVVIAMNEAPPK 441
            L   SPLPI+VVIAM+EA PK
Sbjct: 421 KLPRLSPLPILVVIAMDEATPK 420

BLAST of Chy8G148370 vs. ExPASy TrEMBL
Match: A0A6J1DM67 (WAT1-related protein At5g07050-like OS=Momordica charantia OX=3673 GN=LOC111021737 PE=3 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 8.1e-183
Identity = 358/443 (80.81%), Postives = 390/443 (88.04%), Query Frame = 0

Query: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVL 60
           MEGKG+GIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQ FAT+ L
Sbjct: 1   MEGKGNGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQTFATVAL 60

Query: 61  APFAFFFERKMRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120
           APFAFF ERK+RPKI+F + MQI LLGLLGPVIDQNFYYAGLKLTS TFSCA SNMLPAM
Sbjct: 61  APFAFFLERKVRPKITFPLFMQILLLGLLGPVIDQNFYYAGLKLTSPTFSCAMSNMLPAM 120

Query: 121 TFILALLCRLEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSS 180
           TFILALLCR+EKLEMKKV+CQAKVVGT+VTVGGAILMTLYKGNVISFFWSHH   YLHSS
Sbjct: 121 TFILALLCRMEKLEMKKVKCQAKVVGTMVTVGGAILMTLYKGNVISFFWSHH---YLHSS 180

Query: 181 SASSNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGTL 240
           +      S ESTYQDW+KGSILLLFANLAWA FFI QAMTL+ YTAHLSLTTL+CF GTL
Sbjct: 181 A------SMESTYQDWVKGSILLLFANLAWASFFIFQAMTLKKYTAHLSLTTLVCFLGTL 240

Query: 241 QSMAVTFVME-HKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTP 300
           QSMAVTFVME +K+SVW IGWDMNLLA+VYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTP
Sbjct: 241 QSMAVTFVMENNKSSVWTIGWDMNLLAAVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTP 300

Query: 301 MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYK-EKEAIIEETTIVEP 360
           MIMIIVAIMGSFMLAEKIYIGRVVGG++MVVGLYSVLWGKY+DYK +KEA++EE  IVEP
Sbjct: 301 MIMIIVAIMGSFMLAEKIYIGRVVGGVLMVVGLYSVLWGKYRDYKDQKEAVLEEAAIVEP 360

Query: 361 VKLLISEDKLEKNKKKRLATVV-EKEEEEEETTASTSLNDIEMQRNETTSNVDDNNNNNV 420
           VKL+I E K+     K+LATVV E+EEEEEE   S+SLNDIEMQRN+TTS V       +
Sbjct: 361 VKLIICEAKV----GKKLATVVEEEEEEEEEKPTSSSLNDIEMQRNDTTSIV------GI 420

Query: 421 ATLRCPSPLPIVVVIAMNEAPPK 441
           +    PSP P ++VIAM +APPK
Sbjct: 421 SVSILPSPPPPIIVIAMQKAPPK 424

BLAST of Chy8G148370 vs. NCBI nr
Match: XP_004144320.1 (WAT1-related protein At5g07050 [Cucumis sativus] >KAE8649663.1 hypothetical protein Csa_012952 [Cucumis sativus])

HSP 1 Score: 809 bits (2090), Expect = 2.48e-294
Identity = 432/444 (97.30%), Postives = 438/444 (98.65%), Query Frame = 0

Query: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVL 60
           MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVL
Sbjct: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVL 60

Query: 61  APFAFFFERKMRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120
           APFAFFFERK+RPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM
Sbjct: 61  APFAFFFERKVRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120

Query: 121 TFILALLCRLEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSS 180
           TFILALLCR+EKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSS
Sbjct: 121 TFILALLCRMEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSS 180

Query: 181 SASSNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGTL 240
           SASSNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTL+CFFGTL
Sbjct: 181 SASSNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLVCFFGTL 240

Query: 241 QSMAVTFVMEHKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPM 300
           QSMAVTFVMEHKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPM
Sbjct: 241 QSMAVTFVMEHKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPM 300

Query: 301 IMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEE-TTIVEPV 360
           IMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEE TTIVEPV
Sbjct: 301 IMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEEITTIVEPV 360

Query: 361 KLLISEDKLEKNKKKRLATVVEKEEEEEETTASTSLNDIEMQRNETTSNVDDNNNNNVAT 420
           KLLISEDKLEKNKKK+LATVVE   EEEETT STSLNDIEMQRN+T SNVDDNN+NNVAT
Sbjct: 361 KLLISEDKLEKNKKKKLATVVE---EEEETTTSTSLNDIEMQRNDTRSNVDDNNHNNVAT 420

Query: 421 LRCPSPLPIVVVIAMNEAPPKVFY 443
           LRCPSPLPIVVVIAMNEAPPKVFY
Sbjct: 421 LRCPSPLPIVVVIAMNEAPPKVFY 441

BLAST of Chy8G148370 vs. NCBI nr
Match: XP_008455745.1 (PREDICTED: WAT1-related protein At5g07050-like [Cucumis melo] >KAA0025837.1 WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 758 bits (1958), Expect = 3.56e-274
Identity = 410/442 (92.76%), Postives = 424/442 (95.93%), Query Frame = 0

Query: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVL 60
           MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNI+TKVALNRGMSHYVLVTYRQAFATI L
Sbjct: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIVTKVALNRGMSHYVLVTYRQAFATIAL 60

Query: 61  APFAFFFERKMRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120
           APFAFF ERK+RPKISF MLMQIFLLG LGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM
Sbjct: 61  APFAFFLERKVRPKISFTMLMQIFLLGFLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120

Query: 121 TFILALLCRLEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNY-LHS 180
           TFILALLCR+EKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNY L S
Sbjct: 121 TFILALLCRMEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYDLQS 180

Query: 181 SSASSNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGT 240
           SSASSNYYSFEST QDWLKGSILLLFANLAWALFFI+QA+TLRNYTAHLSLTTL+CFFGT
Sbjct: 181 SSASSNYYSFESTNQDWLKGSILLLFANLAWALFFIIQAITLRNYTAHLSLTTLVCFFGT 240

Query: 241 LQSMAVTFVMEHKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTP 300
           LQSMAVTFVMEH+ SVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIM+KRGPVFVTAFTP
Sbjct: 241 LQSMAVTFVMEHQTSVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMRKRGPVFVTAFTP 300

Query: 301 MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV 360
           MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV
Sbjct: 301 MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV 360

Query: 361 KLLISEDKLEKNKKKRLATVVEKEEEEEE-TTASTSLNDIEMQRNETTSNVDDNNNNNVA 420
           KLLIS DK EKNKKK+LATVVE+EEEEEE TT ST+LNDIEMQRN+TTSNVDDNNNN VA
Sbjct: 361 KLLISVDKFEKNKKKKLATVVEEEEEEEEETTTSTALNDIEMQRNDTTSNVDDNNNN-VA 420

Query: 421 TLRCPSPLPIVVVIAMNEAPPK 440
            L   SPLPI+VVIAM+EA PK
Sbjct: 421 KLPRLSPLPILVVIAMDEATPK 441

BLAST of Chy8G148370 vs. NCBI nr
Match: XP_038881326.1 (WAT1-related protein At5g07050-like [Benincasa hispida])

HSP 1 Score: 735 bits (1897), Expect = 5.35e-265
Identity = 400/443 (90.29%), Postives = 419/443 (94.58%), Query Frame = 0

Query: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVL 60
           MEGKG+GIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATI L
Sbjct: 1   MEGKGNGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIAL 60

Query: 61  APFAFFFERKMRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120
           APFAFF ERK+RPKISF MLMQIFLLGLLGPVIDQNFYYAGLKLTS TFSCA SNMLPAM
Sbjct: 61  APFAFFLERKVRPKISFPMLMQIFLLGLLGPVIDQNFYYAGLKLTSPTFSCAMSNMLPAM 120

Query: 121 TFILALLCRLEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSS 180
           TFILALLCR+EKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHN  YLHSS
Sbjct: 121 TFILALLCRMEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHN--YLHSS 180

Query: 181 SASSNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGTL 240
           + SSNYYSFESTYQDW+KGSILLLFANLAWALFFI+QAMTLRNYTAHLSLTTL+CFFGTL
Sbjct: 181 T-SSNYYSFESTYQDWVKGSILLLFANLAWALFFIIQAMTLRNYTAHLSLTTLVCFFGTL 240

Query: 241 QSMAVTFVMEHKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPM 300
           QSMAVTFVME+KASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPM
Sbjct: 241 QSMAVTFVMENKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPM 300

Query: 301 IMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEE-TTIVEPV 360
           IMIIVAIMGSFMLAEKIYIGRVVGG+VMVVGLYSVLWGKYKDYKEKEAIIEE +TIVEPV
Sbjct: 301 IMIIVAIMGSFMLAEKIYIGRVVGGVVMVVGLYSVLWGKYKDYKEKEAIIEEISTIVEPV 360

Query: 361 KLLISEDKL-EKNKKKRLATVVEKEEEEEETTASTSLNDIEMQRNETTSNVDDNNNNNVA 420
           KLLISE KL E+ KKK+LAT++E+++EEE  T STS NDIE QRN+TTSNVD NNNNNV 
Sbjct: 361 KLLISEGKLVEQKKKKKLATIIEEQQEEE--TTSTSSNDIETQRNDTTSNVD-NNNNNVT 420

Query: 421 TLRCPSPLPIVVVIAMNEAPPKV 441
            L CP PLPI+VVI M EAPPKV
Sbjct: 421 NLLCPPPLPIIVVIPMVEAPPKV 437

BLAST of Chy8G148370 vs. NCBI nr
Match: TYK09619.1 (WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 713 bits (1840), Expect = 1.52e-256
Identity = 392/442 (88.69%), Postives = 405/442 (91.63%), Query Frame = 0

Query: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVL 60
           MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNI+TKVALNRGMSHYVLVTYRQAFATI L
Sbjct: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIVTKVALNRGMSHYVLVTYRQAFATIAL 60

Query: 61  APFAFFFERKMRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120
           APFAFF ER                     PVIDQNFYYAGLKLTSTTFSCATSNMLPAM
Sbjct: 61  APFAFFLER---------------------PVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120

Query: 121 TFILALLCRLEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNY-LHS 180
           TFILALLCR+EKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNY L S
Sbjct: 121 TFILALLCRMEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYDLQS 180

Query: 181 SSASSNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGT 240
           SSASSNYYSFEST QDWLKGSILLLFANLAWALFFI+QA+TLRNYTAHLSLTTL+CFFGT
Sbjct: 181 SSASSNYYSFESTNQDWLKGSILLLFANLAWALFFIIQAITLRNYTAHLSLTTLVCFFGT 240

Query: 241 LQSMAVTFVMEHKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTP 300
           LQSMAVTFVMEH+ SVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIM+KRGPVFVTAFTP
Sbjct: 241 LQSMAVTFVMEHQTSVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMRKRGPVFVTAFTP 300

Query: 301 MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV 360
           MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV
Sbjct: 301 MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPV 360

Query: 361 KLLISEDKLEKNKKKRLATVVEKEEEEEE-TTASTSLNDIEMQRNETTSNVDDNNNNNVA 420
           KLLIS DK EKNKKK+LATVVE+EEEEEE TT ST+LNDIEMQRN+TTSNVDDNNNN VA
Sbjct: 361 KLLISVDKFEKNKKKKLATVVEEEEEEEEETTTSTALNDIEMQRNDTTSNVDDNNNN-VA 420

Query: 421 TLRCPSPLPIVVVIAMNEAPPK 440
            L   SPLPI+VVIAM+EA PK
Sbjct: 421 KLPRLSPLPILVVIAMDEATPK 420

BLAST of Chy8G148370 vs. NCBI nr
Match: XP_022154464.1 (WAT1-related protein At5g07050-like [Momordica charantia])

HSP 1 Score: 648 bits (1672), Expect = 6.14e-231
Identity = 358/443 (80.81%), Postives = 390/443 (88.04%), Query Frame = 0

Query: 1   MEGKGSGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVL 60
           MEGKG+GIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQ FAT+ L
Sbjct: 1   MEGKGNGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQTFATVAL 60

Query: 61  APFAFFFERKMRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAM 120
           APFAFF ERK+RPKI+F + MQI LLGLLGPVIDQNFYYAGLKLTS TFSCA SNMLPAM
Sbjct: 61  APFAFFLERKVRPKITFPLFMQILLLGLLGPVIDQNFYYAGLKLTSPTFSCAMSNMLPAM 120

Query: 121 TFILALLCRLEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSS 180
           TFILALLCR+EKLEMKKV+CQAKVVGT+VTVGGAILMTLYKGNVISFFWSHH   YLHSS
Sbjct: 121 TFILALLCRMEKLEMKKVKCQAKVVGTMVTVGGAILMTLYKGNVISFFWSHH---YLHSS 180

Query: 181 SASSNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGTL 240
           +      S ESTYQDW+KGSILLLFANLAWA FFI QAMTL+ YTAHLSLTTL+CF GTL
Sbjct: 181 A------SMESTYQDWVKGSILLLFANLAWASFFIFQAMTLKKYTAHLSLTTLVCFLGTL 240

Query: 241 QSMAVTFVMEH-KASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTP 300
           QSMAVTFVME+ K+SVW IGWDMNLLA+VYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTP
Sbjct: 241 QSMAVTFVMENNKSSVWTIGWDMNLLAAVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTP 300

Query: 301 MIMIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKE-KEAIIEETTIVEP 360
           MIMIIVAIMGSFMLAEKIYIGRVVGG++MVVGLYSVLWGKY+DYK+ KEA++EE  IVEP
Sbjct: 301 MIMIIVAIMGSFMLAEKIYIGRVVGGVLMVVGLYSVLWGKYRDYKDQKEAVLEEAAIVEP 360

Query: 361 VKLLISEDKLEKNKKKRLATVVEKEEEEEETT-ASTSLNDIEMQRNETTSNVDDNNNNNV 420
           VKL+I E K+ K    +LATVVE+EEEEEE    S+SLNDIEMQRN+TTS V       +
Sbjct: 361 VKLIICEAKVGK----KLATVVEEEEEEEEEKPTSSSLNDIEMQRNDTTSIVG------I 420

Query: 421 ATLRCPSPLPIVVVIAMNEAPPK 440
           +    PSP P ++VIAM +APPK
Sbjct: 421 SVSILPSPPPPIIVIAMQKAPPK 424

BLAST of Chy8G148370 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 435.6 bits (1119), Expect = 4.6e-122
Identity = 229/383 (59.79%), Postives = 299/383 (78.07%), Query Frame = 0

Query: 6   SGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVLAPFAF 65
           S   +F+  ++PY AMISLQFGYAGMNIITK++LN GMSHYVLV YR A AT V+APFAF
Sbjct: 7   SSCESFLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAF 66

Query: 66  FFERKMRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAMTFILA 125
           FFERK +PKI+F++ MQ+F+LGLLGPVIDQNFYY GLK TS TFSCA SNMLPAMTFILA
Sbjct: 67  FFERKAQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILA 126

Query: 126 LLCRLEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLH---SSSA 185
           +L R+E L++KK+ CQAK+ GT+VTV GA+LMT+YKG ++  FW+     Y+H   SS A
Sbjct: 127 VLFRMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWT----KYMHIQDSSHA 186

Query: 186 SSNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAH-LSLTTLMCFFGTLQ 245
           ++      S+ +++LKGSILL+FA LAWA  F++QA  L+ Y  H LSLTTL+CF GTLQ
Sbjct: 187 NTTSSKNSSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQ 246

Query: 246 SMAVTFVMEHKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPMI 305
           ++AVTFVMEH  S W IGWDMNLLA+ Y+GIV+SSI+YYVQG++M+KRGPVF TAF+P++
Sbjct: 247 AVAVTFVMEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLM 306

Query: 306 MIIVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYK----EKEAIIEETTIVE 365
           M+IVA+MGSF+LAEKI++G V+G +++V+GLY+VLWGK K+ +    E   I   + + E
Sbjct: 307 MVIVAVMGSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTE 366

Query: 366 PVKLLISEDKLEKNKKKRLATVV 381
            V+   S+ K+ +     L+T+V
Sbjct: 367 DVEANGSKMKISEGDNSMLSTIV 385

BLAST of Chy8G148370 vs. TAIR 10
Match: AT2G40900.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 382.1 bits (980), Expect = 6.1e-106
Identity = 215/382 (56.28%), Postives = 280/382 (73.30%), Query Frame = 0

Query: 13  EGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVLAPFAFFFERKMR 72
           E A+PY AM+ LQFGYAGMN++TK  L+RGMSHYVLV YR AFAT  +APFA   ERK+R
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 73  PKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAMTFILALLCRLEK 132
            K++F + M+IFLL LLGPVIDQN YY GLKLTS TFS A SN++PA+T ILA L R+EK
Sbjct: 67  SKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEK 126

Query: 133 LEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSSSASSNYYSFEST 192
           +EM+KVRC  KV+GTLVTV G+ILM  YKG  I+FF S       H ++ASS        
Sbjct: 127 VEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFFRS-------HLTAASS------PP 186

Query: 193 YQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGTLQSMAVTFVMEHK 252
             D+LK ++ LL A+L+WA FF++QA TL+ Y+AHLS++T++CF GTLQS+A+ FVMEH 
Sbjct: 187 TADYLKAAVFLLLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHN 246

Query: 253 ASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPMIMIIVAIMGSFM 312
            S  NIG+DMNLLAS YAGI+SSSIAYYVQG++MQ++GPVFVTAF P+I++IV+IM  F+
Sbjct: 247 PSALNIGFDMNLLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFV 306

Query: 313 LAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPVKLLISEDKLEKNK 372
           L + IY+G V+G +V++VG+Y+VLWGK+ D   +E   E+  +   VK     + L    
Sbjct: 307 LGQGIYLGGVIGVVVLMVGVYAVLWGKHVDDDGEETRHEDNVVA--VKCCSGNNGLTIMP 366

Query: 373 KKRLATVVEKEEEEEETTASTS 395
           K     + E +EE+ ET  +TS
Sbjct: 367 K-----IDEADEEDVETGKATS 368

BLAST of Chy8G148370 vs. TAIR 10
Match: AT3G56620.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 369.0 bits (946), Expect = 5.3e-102
Identity = 197/348 (56.61%), Postives = 264/348 (75.86%), Query Frame = 0

Query: 13  EGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVLAPFAFFFERKMR 72
           E A+PY AM+ LQFGYAGMN++TKV L+RGMSHYVLV YR AFAT  +APFA   ERK+R
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKVVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 73  PKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAMTFILALLCRLEK 132
           PK++F + MQIF+L LLGP+IDQN YYAGLKLTS TF+ A +N++PA+TFI++++CR+EK
Sbjct: 67  PKMTFPIFMQIFVLALLGPLIDQNLYYAGLKLTSPTFAGAVTNIVPALTFIISIICRMEK 126

Query: 133 LEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSSSASSNYYSFEST 192
           +EM+KVR QAKVVGTLV V GA+LM L+K  +I+F  SH   + L  +            
Sbjct: 127 VEMRKVRFQAKVVGTLVIVVGAMLMILFKIPLITFLRSHLTGHALSPAG----------- 186

Query: 193 YQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGTLQSMAVTFVMEHK 252
            +D+LK ++ LL A+ +WA FF++QA TL+ Y++HLSL+T++CF GTLQS A+TFVME  
Sbjct: 187 -EDYLKATVFLLIASFSWASFFVLQAATLKRYSSHLSLSTMVCFMGTLQSTALTFVMEPN 246

Query: 253 ASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPMIMIIVAIMGSFM 312
            S WNIG+DMNLLAS YAGI+SSSIAYYVQGM+ +++  +FVTAF P+++II +I+G  +
Sbjct: 247 LSAWNIGFDMNLLASAYAGIMSSSIAYYVQGMMTKQKSVIFVTAFNPLVVIIGSIIGFLI 306

Query: 313 LAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYKEKEAIIEETTIVEPVK 361
           L + + +G V+G  ++VVG+ +VLWGK  D  E+E I  E   VE VK
Sbjct: 307 LNQTLNLGGVLGMAILVVGVCTVLWGKEGDIDEEENI--EEKFVEIVK 340

BLAST of Chy8G148370 vs. TAIR 10
Match: AT1G21890.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 323.2 bits (827), Expect = 3.3e-88
Identity = 181/399 (45.36%), Postives = 261/399 (65.41%), Query Frame = 0

Query: 5   GSGIANFVEGAQPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVLAPFA 64
           G G+ N     +PY+AMIS+QFGYAGM IIT V+L  GM+HYVL  YR A AT V+APFA
Sbjct: 2   GRGLMN---SLKPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFA 61

Query: 65  FFFERKMRPKISFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAMTFIL 124
            F ERK+RPK++F + +QI LLG + PV+DQN YY G+  TS TF+ AT+N+LPA+TF+L
Sbjct: 62  LFHERKIRPKMTFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVL 121

Query: 125 ALLCRLEKLEMKKVRCQAKVVGTLVTVGGAILMTLYKGNVISFF-WSHHNNNYLHSSSAS 184
           A++ RLE +  KKVR  AKVVGT++TV GA+LMTLYKG ++ F  +          +  S
Sbjct: 122 AIIFRLESVNFKKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGS 181

Query: 185 SNYYSFESTYQDWLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGTLQSM 244
                  +  + W+ G+++LL     WA FFI+Q+ TL+ Y A LSLTTL+C  GTL+  
Sbjct: 182 HGGAGAAAMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGT 241

Query: 245 AVTFVMEHKASVWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPMIMI 304
           AV+ V     S W IG+D NL A+ Y+G++ S +AYYVQG++M++RGPVFV  F P+ ++
Sbjct: 242 AVSLVTVRDLSAWKIGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVV 301

Query: 305 IVAIMGSFMLAEKIYIGRVVGGIVMVVGLYSVLWGKYKDYK--EKEAIIEETTIVEPVKL 364
           I A +G  +L+E I++G V+G + ++VGLY+V+WGK KD +  + +   +   I  PVK 
Sbjct: 302 ITAALGVVVLSESIHLGSVIGTLFIIVGLYTVVWGKGKDKRMTDDDEDCKGLPIKSPVKP 361

Query: 365 LISEDKLEKNKKKRLATVVEKEEEEEETTASTSLNDIEM 401
           +        +  K LA  +E + +E +   +T+   +E+
Sbjct: 362 V--------DTGKGLAAELEMKSKEGQEAKATTTTQVEI 389

BLAST of Chy8G148370 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 317.4 bits (812), Expect = 1.8e-86
Identity = 175/353 (49.58%), Postives = 244/353 (69.12%), Query Frame = 0

Query: 16  QPYIAMISLQFGYAGMNIITKVALNRGMSHYVLVTYRQAFATIVLAPFAFFFERKMRPKI 75
           +P+I ++SLQFGYAG++II K ALN+GMS +VL +YR   ATI +APFA+F +RK+RPK+
Sbjct: 7   KPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPKM 66

Query: 76  SFAMLMQIFLLGLLGPVIDQNFYYAGLKLTSTTFSCATSNMLPAMTFILALLCRLEKLEM 135
           + ++  +I LLGLL P IDQN YY G+K TS TF+ A +N+LPA  FI+A + RLEK+ +
Sbjct: 67  TLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNV 126

Query: 136 KKVRCQAKVVGTLVTVGGAILMTLYKGNVISFFWSHHNNNYLHSSSASSNYYSFESTYQD 195
           KK+  QAK++GT+VTVGGA+LMT+ KG +I   W+  N + +H  S+++         QD
Sbjct: 127 KKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWA--NPHDIHQDSSNT------GVKQD 186

Query: 196 WLKGSILLLFANLAWALFFIVQAMTLRNYTAHLSLTTLMCFFGTLQSMAVT-FVMEHKAS 255
             KG+ L+    + WA F  +QA+TL++Y   LSLT  +CF G+++S  V  F+     S
Sbjct: 187 LTKGASLIAIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPS 246

Query: 256 VWNIGWDMNLLASVYAGIVSSSIAYYVQGMIMQKRGPVFVTAFTPMIMIIVAIMGSFMLA 315
            W I  D  LLA+VY G++ S I YYVQG+IM+ RGPVFVTAF P+ M+IVAI+GS +LA
Sbjct: 247 AWAIHLDSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILA 306

Query: 316 EKIYIGRVVGGIVMVVGLYSVLWGKYKDY-------KEKEAIIEETTIVEPVK 361
           E +++GR++G IV+V+GLYSVLWGK KD         +KE  +    IV P K
Sbjct: 307 EVMFLGRILGAIVIVLGLYSVLWGKSKDEPSSSFSDMDKELPLSTPQIVLPSK 351

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FL416.5e-12159.79WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2... [more]
F4IJ088.6e-10556.28WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2... [more]
Q9LXX87.5e-10156.61WAT1-related protein At3g56620 OS=Arabidopsis thaliana OX=3702 GN=At3g56620 PE=2... [more]
F4HZQ74.7e-8745.36WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2... [more]
O806382.6e-8549.58WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0KYE03.2e-21997.17Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G420210 PE=3 SV=1[more]
A0A5A7SM955.6e-21692.76WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold34G... [more]
A0A1S3C1665.6e-21692.76WAT1-related protein At5g07050-like OS=Cucumis melo OX=3656 GN=LOC103495845 PE=3... [more]
A0A5D3CH742.7e-20288.69WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447... [more]
A0A6J1DM678.1e-18380.81WAT1-related protein At5g07050-like OS=Momordica charantia OX=3673 GN=LOC1110217... [more]
Match NameE-valueIdentityDescription
XP_004144320.12.48e-29497.30WAT1-related protein At5g07050 [Cucumis sativus] >KAE8649663.1 hypothetical prot... [more]
XP_008455745.13.56e-27492.76PREDICTED: WAT1-related protein At5g07050-like [Cucumis melo] >KAA0025837.1 WAT1... [more]
XP_038881326.15.35e-26590.29WAT1-related protein At5g07050-like [Benincasa hispida][more]
TYK09619.11.52e-25688.69WAT1-related protein [Cucumis melo var. makuwa][more]
XP_022154464.16.14e-23180.81WAT1-related protein At5g07050-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G07050.14.6e-12259.79nodulin MtN21 /EamA-like transporter family protein [more]
AT2G40900.16.1e-10656.28nodulin MtN21 /EamA-like transporter family protein [more]
AT3G56620.15.3e-10256.61nodulin MtN21 /EamA-like transporter family protein [more]
AT1G21890.13.3e-8845.36nodulin MtN21 /EamA-like transporter family protein [more]
AT2G39510.11.8e-8649.58nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 198..336
e-value: 7.6E-12
score: 45.6
coord: 18..157
e-value: 1.3E-12
score: 48.0
NoneNo IPR availablePANTHERPTHR31218:SF257WAT1-RELATED PROTEINcoord: 11..371
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 51..161
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 11..371

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy8G148370.1Chy8G148370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity