Chy8G148350 (gene) Cucumber (hystrix) v1

Overview


Name	Chy8G148350
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Cysteine proteinase inhibitor
Location	chrH08: 5187247 .. 5187838 (+)
RNA-Seq Expression	Chy8G148350
Synteny	Chy8G148350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACTGAATCACATAGCTGTGCCATTGCTATTTCTTCTTCTTGCTGCCTTCGATTTGGGGTTTTCCGTCGAAGACCCATTTATCAGGATGAAACTGGGAGGCGTTCGCGATTACATCGGCGCCCAGAACAGCGTTGAAATCGACTCTCTCGCTCGTTTTGCAGTTCAAGAACACAACAACAAAGAGGTATTTTCCTTTGTTATCTTAAGTTTTACTTCTCCTCACTCCCCTTTTCCTTATATTGGTATTGTTTAATTTCTGGGTACGTCTCAATCTCTCCTTTTCTTTATTGTTCCGCTTAGAATCGAGTTAATTTCTCACTTAATTCGTTACCCATGTTTCCGATTTCTCATGGGTGCTTTCGATTTGTTTTAATCACCAATTCTGAGTTCTTGTTTCTTTGGTACAGAACGGTCTTTTGGAATTTGAGCGAGTGTTGAAAGCTAAAGAACAGATAGTTGCTGGTAAATTGTATCATCTTACATTGGAAGCCATTGATGGTGGCAAGAAGAAGGTTTATGAAGCCAAAGTGTGGGTGAAGCCTTGGATGAACTTCCAACAGTTGCAGGAATTCAAGCTATGCACATGA

mRNA sequence

ATGAAACTGAATCACATAGCTGTGCCATTGCTATTTCTTCTTCTTGCTGCCTTCGATTTGGGGTTTTCCGTCGAAGACCCATTTATCAGGATGAAACTGGGAGGCGTTCGCGATTACATCGGCGCCCAGAACAGCGTTGAAATCGACTCTCTCGCTCGTTTTGCAGTTCAAGAACACAACAACAAAGAGAACGGTCTTTTGGAATTTGAGCGAGTGTTGAAAGCTAAAGAACAGATAGTTGCTGGTAAATTGTATCATCTTACATTGGAAGCCATTGATGGTGGCAAGAAGAAGGTTTATGAAGCCAAAGTGTGGGTGAAGCCTTGGATGAACTTCCAACAGTTGCAGGAATTCAAGCTATGCACATGA

Coding sequence (CDS)

ATGAAACTGAATCACATAGCTGTGCCATTGCTATTTCTTCTTCTTGCTGCCTTCGATTTGGGGTTTTCCGTCGAAGACCCATTTATCAGGATGAAACTGGGAGGCGTTCGCGATTACATCGGCGCCCAGAACAGCGTTGAAATCGACTCTCTCGCTCGTTTTGCAGTTCAAGAACACAACAACAAAGAGAACGGTCTTTTGGAATTTGAGCGAGTGTTGAAAGCTAAAGAACAGATAGTTGCTGGTAAATTGTATCATCTTACATTGGAAGCCATTGATGGTGGCAAGAAGAAGGTTTATGAAGCCAAAGTGTGGGTGAAGCCTTGGATGAACTTCCAACAGTTGCAGGAATTCAAGCTATGCACATGA

Protein sequence

MKLNHIAVPLLFLLLAAFDLGFSVEDPFIRMKLGGVRDYIGAQNSVEIDSLARFAVQEHNNKENGLLEFERVLKAKEQIVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFKLCT*

Homology

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BLAST of Chy8G148350 vs. ExPASy Swiss-Prot
Match: Q8H0X6 (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=2)

HSP 1 Score: 136.0 bits (341), Expect = 3.0e-31
Identity = 69/113 (61.06%), Postives = 86/113 (76.11%), Query Frame = 0


Query: 7   AVPLLFLLLAAFDLGFSVEDPFIRMKLGGVRDYIGAQNSVEIDSLARFAVQEHNNKENGL 66
           ++ L    L A DLGF  E+  +   +GGV D    QNS E++SLARFAV EHN KEN L
Sbjct: 14  SLSLFISSLIASDLGFCNEEMAL---VGGVGDVPANQNSGEVESLARFAVDEHNKKENAL 73

Query: 67  LEFERVLKAKEQIVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFK 120
           LEF RV+KAKEQ+VAG L+HLTLE ++ G+KK+YEAKVWVKPW+NF++LQEFK
Sbjct: 74  LEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEFK 123

[Close]

BLAST of Chy8G148350 vs. ExPASy Swiss-Prot
Match: Q06445 (Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 3.3e-30
Identity = 62/87 (71.26%), Postives = 75/87 (86.21%), Query Frame = 0


Query: 33  LGGVRDYIGAQNSVEIDSLARFAVQEHNNKENGLLEFERVLKAKEQIVAGKLYHLTLEAI 92
           LGG RD  G QNS+EIDSLARFAV+EHN K+N LLEF RV+ A++Q+V+G LY +TLEA 
Sbjct: 4   LGGNRDVAGNQNSLEIDSLARFAVEEHNKKQNALLEFGRVVSAQQQVVSGTLYTITLEAK 63

Query: 93  DGGKKKVYEAKVWVKPWMNFQQLQEFK 120
           DGG+KKVYEAKVW KPW+NF++LQEFK
Sbjct: 64  DGGQKKVYEAKVWEKPWLNFKELQEFK 90

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BLAST of Chy8G148350 vs. ExPASy Swiss-Prot
Match: Q0JNR2 (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 9.5e-30
Identity = 73/120 (60.83%), Postives = 88/120 (73.33%), Query Frame = 0


Query: 7   AVPL-LFLLL---AAFDLGFSVEDPFIRMK---LGGVRDYIGAQNSVEIDSLARFAVQEH 66
           A PL LFLLL   AA    F V    + M    LGG  D   A NSVE D+LARFAV EH
Sbjct: 15  AAPLPLFLLLAVAAAAAALFLVGSASLAMAGHVLGGAHDAPSAANSVETDALARFAVDEH 74

Query: 67  NNKENGLLEFERVLKAKEQIVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFK 120
           N +EN LLEF RV++AKEQ+VAG L+HLTLEA++ G+KKVYEAKVWVKPW++F++LQEF+
Sbjct: 75  NKRENALLEFVRVVEAKEQVVAGTLHHLTLEALEAGRKKVYEAKVWVKPWLDFKELQEFR 134

[Close]

BLAST of Chy8G148350 vs. ExPASy Swiss-Prot
Match: Q41906 (Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=2)

HSP 1 Score: 130.6 bits (327), Expect = 1.2e-29
Identity = 60/89 (67.42%), Postives = 76/89 (85.39%), Query Frame = 0


Query: 31  MKLGGVRDYIGAQNSVEIDSLARFAVQEHNNKENGLLEFERVLKAKEQIVAGKLYHLTLE 90
           M LGGV D  G QNS EI+SLARFA+QEHN ++N +LEF++++KA+EQ+VAG +YHLTLE
Sbjct: 33  MMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQNKILEFKKIVKAREQVVAGTMYHLTLE 92

Query: 91  AIDGGKKKVYEAKVWVKPWMNFQQLQEFK 120
           A +G + K +EAKVWVKPWMNF+QLQEFK
Sbjct: 93  AKEGDQTKNFEAKVWVKPWMNFKQLQEFK 121

[Close]

BLAST of Chy8G148350 vs. ExPASy Swiss-Prot
Match: Q8LC76 (Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=2)

HSP 1 Score: 127.9 bits (320), Expect = 8.1e-29
Identity = 63/95 (66.32%), Postives = 73/95 (76.84%), Query Frame = 0


Query: 26  DPFIRMKLGGVRDYIGAQN-SVEIDSLARFAVQEHNNKENGLLEFERVLKAKEQIVAGKL 85
           D  ++MKLGG  D     N   EID +A FAVQEHN +EN +LE  RVLKA EQ+VAGKL
Sbjct: 38  DNVVKMKLGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKL 97

Query: 86  YHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFK 120
           Y LTLE I+ G+KK+YEAKVWVKPWMNF+QLQEFK
Sbjct: 98  YRLTLEVIEAGEKKIYEAKVWVKPWMNFKQLQEFK 132

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BLAST of Chy8G148350 vs. ExPASy TrEMBL
Match: A0A1S4E0R5 (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103495847 PE=3 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.0e-59
Identity = 115/122 (94.26%), Postives = 121/122 (99.18%), Query Frame = 0


Query: 1   MKLNHIAVPLLFLLLAAFDLGFSVEDPFIRMKLGGVRDYIGAQNSVEIDSLARFAVQEHN 60
           MKLNHIAVPLLFLLLAAFDLGFS+EDPFIRMKLGGVRDYIG+QN VEI+SLARFAVQEHN
Sbjct: 1   MKLNHIAVPLLFLLLAAFDLGFSIEDPFIRMKLGGVRDYIGSQNRVEIESLARFAVQEHN 60

Query: 61  NKENGLLEFERVLKAKEQIVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFKL 120
           NKENGLLEF+RVLKAKEQ+VAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNF+QLQEFKL
Sbjct: 61  NKENGLLEFDRVLKAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKL 120

Query: 121 CT 123
           CT
Sbjct: 121 CT 122

[Close]

BLAST of Chy8G148350 vs. ExPASy TrEMBL
Match: A0A5D3CEB5 (Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00160 PE=3 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.0e-59
Identity = 115/122 (94.26%), Postives = 121/122 (99.18%), Query Frame = 0


Query: 1   MKLNHIAVPLLFLLLAAFDLGFSVEDPFIRMKLGGVRDYIGAQNSVEIDSLARFAVQEHN 60
           MKLNHIAVPLLFLLLAAFDLGFS+EDPFIRMKLGGVRDYIG+QN VEI+SLARFAVQEHN
Sbjct: 1   MKLNHIAVPLLFLLLAAFDLGFSIEDPFIRMKLGGVRDYIGSQNRVEIESLARFAVQEHN 60

Query: 61  NKENGLLEFERVLKAKEQIVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFKL 120
           NKENGLLEF+RVLKAKEQ+VAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNF+QLQEFKL
Sbjct: 61  NKENGLLEFDRVLKAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKL 120

Query: 121 CT 123
           CT
Sbjct: 121 CT 122

[Close]

BLAST of Chy8G148350 vs. ExPASy TrEMBL
Match: A0A6J1HVR4 (Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111467290 PE=3 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 7.0e-52
Identity = 103/122 (84.43%), Postives = 112/122 (91.80%), Query Frame = 0


Query: 1   MKLNHIAVPLLFLLLAAFDLGFSVEDPFIRMKLGGVRDYIGAQNSVEIDSLARFAVQEHN 60
           MK+NHIA  LL LLL  FD G+ VEDPFI+MKLGGVRDYIGAQNS+EID+LARFAVQEHN
Sbjct: 1   MKVNHIAAVLLVLLLPVFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  NKENGLLEFERVLKAKEQIVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFKL 120
            KENGLLEFERVLKAKEQ+V+GKLYHLTLEAIDGG KKVYEAKVWVKPWMNF+QLQEFK 
Sbjct: 61  KKENGLLEFERVLKAKEQVVSGKLYHLTLEAIDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120

Query: 121 CT 123
           C+
Sbjct: 121 CS 122

[Close]

BLAST of Chy8G148350 vs. ExPASy TrEMBL
Match: A0A6J1F2Q3 (Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111439234 PE=3 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 7.0e-52
Identity = 104/121 (85.95%), Postives = 111/121 (91.74%), Query Frame = 0


Query: 1   MKLNHIAVPLLFLLLAAFDLGFSVEDPFIRMKLGGVRDYIGAQNSVEIDSLARFAVQEHN 60
           MK+NHIA  LL LLL  FD G+ VEDPFI+MKLGGVRDYIGAQNS+EID+LARFAVQEHN
Sbjct: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  NKENGLLEFERVLKAKEQIVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFKL 120
            KENGLLEFERVLKAKEQ+VAGKLYHLTLEAIDGG KKVYEAKVWVKPWMNF+QLQEFK 
Sbjct: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120

Query: 121 C 122
           C
Sbjct: 121 C 121

[Close]

BLAST of Chy8G148350 vs. ExPASy TrEMBL
Match: A0A5N6RV79 (Cysteine proteinase inhibitor OS=Carpinus fangiana OX=176857 GN=FH972_019958 PE=3 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.9e-37
Identity = 82/111 (73.87%), Postives = 94/111 (84.68%), Query Frame = 0


Query: 10  LLFLLLAAFDLGF-SVEDPFIRMKLGGVRDYIGAQNSVEIDSLARFAVQEHNNKENGLLE 69
           L+ LL A  +LG+ S E+  IRMKLGGV D  G+QNS EI++LARFAVQEHNNKEN +LE
Sbjct: 18  LVLLLCAVSELGYCSGEESAIRMKLGGVHDCKGSQNSAEIENLARFAVQEHNNKENTILE 77

Query: 70  FERVLKAKEQIVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFK 120
           F RVLKAKEQ+VAGK+YHLTLE ID GKK +YEAKVWVKPWMNF+QLQEFK
Sbjct: 78  FARVLKAKEQVVAGKIYHLTLEVIDAGKKNIYEAKVWVKPWMNFKQLQEFK 128

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BLAST of Chy8G148350 vs. NCBI nr
Match: XP_008455746.1 (PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901818.1 PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901819.1 PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >KAA0025834.1 cysteine proteinase inhibitor A [Cucumis melo var. makuwa] >TYK09622.1 cysteine proteinase inhibitor A [Cucumis melo var. makuwa])

HSP 1 Score: 238 bits (606), Expect = 8.48e-79
Identity = 115/122 (94.26%), Postives = 121/122 (99.18%), Query Frame = 0


Query: 1   MKLNHIAVPLLFLLLAAFDLGFSVEDPFIRMKLGGVRDYIGAQNSVEIDSLARFAVQEHN 60
           MKLNHIAVPLLFLLLAAFDLGFS+EDPFIRMKLGGVRDYIG+QN VEI+SLARFAVQEHN
Sbjct: 1   MKLNHIAVPLLFLLLAAFDLGFSIEDPFIRMKLGGVRDYIGSQNRVEIESLARFAVQEHN 60

Query: 61  NKENGLLEFERVLKAKEQIVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFKL 120
           NKENGLLEF+RVLKAKEQ+VAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNF+QLQEFKL
Sbjct: 61  NKENGLLEFDRVLKAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKL 120

Query: 121 CT 122
           CT
Sbjct: 121 CT 122

[Close]

BLAST of Chy8G148350 vs. NCBI nr
Match: XP_038880981.1 (cysteine proteinase inhibitor A-like [Benincasa hispida])

HSP 1 Score: 225 bits (574), Expect = 1.43e-73
Identity = 111/121 (91.74%), Postives = 115/121 (95.04%), Query Frame = 0


Query: 1   MKLNHIAVPLLFLLLAAFDLGFSVEDPFIRMKLGGVRDYIGAQNSVEIDSLARFAVQEHN 60
           MK+N IAV LL LLLA FDLGFSVEDPFIRMKLGGVRDYIGAQNSVEID LARFAVQEHN
Sbjct: 25  MKVNRIAVALLLLLLAVFDLGFSVEDPFIRMKLGGVRDYIGAQNSVEIDYLARFAVQEHN 84

Query: 61  NKENGLLEFERVLKAKEQIVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFKL 120
            KENGLLEFERVLKAKEQ++AGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNF+QLQEFKL
Sbjct: 85  KKENGLLEFERVLKAKEQVIAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKL 144

[Close]

BLAST of Chy8G148350 vs. NCBI nr
Match: XP_022967919.1 (cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967920.1 cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967921.1 cysteine proteinase inhibitor A-like [Cucurbita maxima])

HSP 1 Score: 213 bits (541), Expect = 6.95e-69
Identity = 103/122 (84.43%), Postives = 112/122 (91.80%), Query Frame = 0


Query: 1   MKLNHIAVPLLFLLLAAFDLGFSVEDPFIRMKLGGVRDYIGAQNSVEIDSLARFAVQEHN 60
           MK+NHIA  LL LLL  FD G+ VEDPFI+MKLGGVRDYIGAQNS+EID+LARFAVQEHN
Sbjct: 1   MKVNHIAAVLLVLLLPVFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  NKENGLLEFERVLKAKEQIVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFKL 120
            KENGLLEFERVLKAKEQ+V+GKLYHLTLEAIDGG KKVYEAKVWVKPWMNF+QLQEFK 
Sbjct: 61  KKENGLLEFERVLKAKEQVVSGKLYHLTLEAIDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120

Query: 121 CT 122
           C+
Sbjct: 121 CS 122

[Close]

BLAST of Chy8G148350 vs. NCBI nr
Match: XP_022932782.1 (cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932783.1 cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932784.1 cysteine proteinase inhibitor A-like [Cucurbita moschata] >KAG7034455.1 hypothetical protein SDJN02_04184 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 212 bits (540), Expect = 9.88e-69
Identity = 104/121 (85.95%), Postives = 111/121 (91.74%), Query Frame = 0


Query: 1   MKLNHIAVPLLFLLLAAFDLGFSVEDPFIRMKLGGVRDYIGAQNSVEIDSLARFAVQEHN 60
           MK+NHIA  LL LLL  FD G+ VEDPFI+MKLGGVRDYIGAQNS+EID+LARFAVQEHN
Sbjct: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  NKENGLLEFERVLKAKEQIVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFKL 120
            KENGLLEFERVLKAKEQ+VAGKLYHLTLEAIDGG KKVYEAKVWVKPWMNF+QLQEFK 
Sbjct: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120

[Close]

BLAST of Chy8G148350 vs. NCBI nr
Match: XP_023543498.1 (cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543500.1 cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543501.1 cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 206 bits (525), Expect = 1.91e-66
Identity = 100/121 (82.64%), Postives = 109/121 (90.08%), Query Frame = 0


Query: 1   MKLNHIAVPLLFLLLAAFDLGFSVEDPFIRMKLGGVRDYIGAQNSVEIDSLARFAVQEHN 60
           MK+NHI   LL LLL  FD G+ V+DPFI+MKLGGVRDYIGAQNS+EID+LARFAVQEHN
Sbjct: 1   MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  NKENGLLEFERVLKAKEQIVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFKL 120
            K+NGLLEFERVLKAKEQ+VAGKLYHLTLEA DGG KKVYEAKVWVKPWMNF+QLQEFK 
Sbjct: 61  KKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120

[Close]

BLAST of Chy8G148350 vs. TAIR 10
Match: AT3G12490.2 (cystatin B )

HSP 1 Score: 136.0 bits (341), Expect = 2.1e-32
Identity = 69/113 (61.06%), Postives = 86/113 (76.11%), Query Frame = 0


Query: 7   AVPLLFLLLAAFDLGFSVEDPFIRMKLGGVRDYIGAQNSVEIDSLARFAVQEHNNKENGL 66
           ++ L    L A DLGF  E+  +   +GGV D    QNS E++SLARFAV EHN KEN L
Sbjct: 14  SLSLFISSLIASDLGFCNEEMAL---VGGVGDVPANQNSGEVESLARFAVDEHNKKENAL 73

Query: 67  LEFERVLKAKEQIVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFK 120
           LEF RV+KAKEQ+VAG L+HLTLE ++ G+KK+YEAKVWVKPW+NF++LQEFK
Sbjct: 74  LEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEFK 123

[Close]

BLAST of Chy8G148350 vs. TAIR 10
Match: AT3G12490.1 (cystatin B )

HSP 1 Score: 131.3 bits (329), Expect = 5.2e-31
Identity = 61/87 (70.11%), Postives = 74/87 (85.06%), Query Frame = 0


Query: 33  LGGVRDYIGAQNSVEIDSLARFAVQEHNNKENGLLEFERVLKAKEQIVAGKLYHLTLEAI 92
           +GGV D    QNS E++SLARFAV EHN KEN LLEF RV+KAKEQ+VAG L+HLTLE +
Sbjct: 4   VGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEIL 63

Query: 93  DGGKKKVYEAKVWVKPWMNFQQLQEFK 120
           + G+KK+YEAKVWVKPW+NF++LQEFK
Sbjct: 64  EAGQKKLYEAKVWVKPWLNFKELQEFK 90

[Close]

BLAST of Chy8G148350 vs. TAIR 10
Match: AT2G40880.1 (cystatin A )

HSP 1 Score: 130.6 bits (327), Expect = 8.8e-31
Identity = 60/89 (67.42%), Postives = 76/89 (85.39%), Query Frame = 0


Query: 31  MKLGGVRDYIGAQNSVEIDSLARFAVQEHNNKENGLLEFERVLKAKEQIVAGKLYHLTLE 90
           M LGGV D  G QNS EI+SLARFA+QEHN ++N +LEF++++KA+EQ+VAG +YHLTLE
Sbjct: 33  MMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQNKILEFKKIVKAREQVVAGTMYHLTLE 92

Query: 91  AIDGGKKKVYEAKVWVKPWMNFQQLQEFK 120
           A +G + K +EAKVWVKPWMNF+QLQEFK
Sbjct: 93  AKEGDQTKNFEAKVWVKPWMNFKQLQEFK 121

[Close]

BLAST of Chy8G148350 vs. TAIR 10
Match: AT5G05110.1 (Cystatin/monellin family protein )

HSP 1 Score: 127.9 bits (320), Expect = 5.7e-30
Identity = 63/95 (66.32%), Postives = 73/95 (76.84%), Query Frame = 0


Query: 26  DPFIRMKLGGVRDYIGAQN-SVEIDSLARFAVQEHNNKENGLLEFERVLKAKEQIVAGKL 85
           D  ++MKLGG  D     N   EID +A FAVQEHN +EN +LE  RVLKA EQ+VAGKL
Sbjct: 38  DNVVKMKLGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKL 97

Query: 86  YHLTLEAIDGGKKKVYEAKVWVKPWMNFQQLQEFK 120
           Y LTLE I+ G+KK+YEAKVWVKPWMNF+QLQEFK
Sbjct: 98  YRLTLEVIEAGEKKIYEAKVWVKPWMNFKQLQEFK 132

[Close]

BLAST of Chy8G148350 vs. TAIR 10
Match: AT5G12140.1 (cystatin-1 )

HSP 1 Score: 98.2 bits (243), Expect = 4.9e-21
Identity = 47/86 (54.65%), Postives = 61/86 (70.93%), Query Frame = 0


Query: 33  LGGVRDYIGAQNSVEIDSLARFAVQEHNNKENGLLEFERVLKAKEQIVAGKLYHLTLEAI 92
           +GGVRD     N ++++SLARFAV EHN  EN  LE++R+L AK Q+VAG ++HLT+E  
Sbjct: 10  VGGVRDIDANANDLQVESLARFAVDEHNKNENLTLEYKRLLGAKTQVVAGTMHHLTVEVA 69

Query: 93  DGGKKKVYEAKVWVKPWMNFQQLQEF 119
           DG   KVYEAKV  K W N +QL+ F
Sbjct: 70  DGETNKVYEAKVLEKAWENLKQLESF 95

The following BLAST results are available for this feature:

BLAST of Chy8G148350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8H0X6	3.0e-31	61.06	Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=...	[more]
Q06445	3.3e-30	71.26	Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1	[more]
Q0JNR2	9.5e-30	60.83	Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
Q41906	1.2e-29	67.42	Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=...	[more]
Q8LC76	8.1e-29	66.32	Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=...	[more]

BLAST of Chy8G148350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4E0R5	2.0e-59	94.26	Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103495847 PE=3 SV=1	[more]
A0A5D3CEB5	2.0e-59	94.26	Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A6J1HVR4	7.0e-52	84.43	Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111467290 PE=3 S...	[more]
A0A6J1F2Q3	7.0e-52	85.95	Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111439234 PE=3...	[more]
A0A5N6RV79	2.9e-37	73.87	Cysteine proteinase inhibitor OS=Carpinus fangiana OX=176857 GN=FH972_019958 PE=...	[more]

BLAST of Chy8G148350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008455746.1	8.48e-79	94.26	PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901818.1 PREDIC...	[more]
XP_038880981.1	1.43e-73	91.74	cysteine proteinase inhibitor A-like [Benincasa hispida]	[more]
XP_022967919.1	6.95e-69	84.43	cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967920.1 cysteine...	[more]
XP_022932782.1	9.88e-69	85.95	cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932783.1 cystei...	[more]
XP_023543498.1	1.91e-66	82.64	cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543500....	[more]

BLAST of Chy8G148350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12490.2	2.1e-32	61.06	cystatin B	[more]
AT3G12490.1	5.2e-31	70.11	cystatin B	[more]
AT2G40880.1	8.8e-31	67.42	cystatin A	[more]
AT5G05110.1	5.7e-30	66.32	Cystatin/monellin family protein	[more]
AT5G12140.1	4.9e-21	54.65	cystatin-1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 31..121 e-value: 1.9E-24 score: 97.2
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 46..120 e-value: 7.2E-22 score: 77.5
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 44..119 e-value: 1.05978E-20 score: 78.2848
None	No IPR available	GENE3D	3.10.450.10		coord: 30..122 e-value: 2.8E-39 score: 135.6
None	No IPR available	PANTHER	PTHR11413:SF110	CYSTEINE PROTEINASE INHIBITOR 7	coord: 31..120
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 30..120
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 31..120
IPR018073	Proteinase inhibitor I25, cystatin, conserved site	PROSITE	PS00287	CYSTATIN	coord: 77..90

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy8G148350.1	Chy8G148350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006972	hyperosmotic response
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009409	response to cold
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0009414	response to water deprivation
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity